AT1G19480

DNA-3-methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007076
Physical Location & Seq
Forward (+)
6744353 .. 6746635
2283 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G19480.2

Sequence Viewer

Length: 1149 bp
ATGGGAGAACAATCACCTTCTCAACCATCGACTCAATGCCAAAGTCATCCACAATCACCCAAACCCGATACTCATAACCTAATCCCACCGGAATCCACCGATGAATGTCTCGATTCAGCTGGAGTTTCAGGCTCAATCGTCTCATCTACCACAATCGACGCTCGACGGATCACTGAACTAGGCAATGTATCGTCTCCGCCGTCCAAAATCCCTCTCCGCCCTCGGAAAATCCGGAAACTCACACTCGACGGAGACGTCTCCGGTGAAGATTATAAGGCGGAGGATATCTCCTCATCTCAAGTAAACTCTCCGCTCGCGACGGATGGAAAATCTCCCGGTAAAGGTAAATTGTCGCACTTACGAGCTATAACTGTGCCGAGGATCCAAGCTCGTCCTCTGACATGTGAAGGTGAGCTCGAAACAGCGATTCACTATCTCCGTAACGCGGATCCTTTGCTCGCGGCGTTAATCGACGTTCATCCACCGCCGACGTTTGAGTCTTTTAAGACTCCGTTCTTAGCTCTGATACGAAACATCCTCTACCAACAGCTTGCAATGAAAGCTGGGAATTCAATCTACACACGATTTGTTTCTCTCTGCGGCGGCGAAAATTTGGTTGTACCTGAGACGGTCTTATCATTAAATCCTCAGCAGCTTCGTCAAATTGGTGTCTCGGGACGTAAGGCGAGTTATCTTCATGATTTAGCTAGAAAGTATCAAAACGGAATCTTGTCGGATTCGGCGATTTTGAACATGGATGAGAAGTCTTTGTTCACGATGCTAACAATGGTTAATGGAATTGGGTCATGGTCTGTTCATATGTTCATGATAAATTCTCTTCATCGACCAGATGTTTTGCCGGTTAATGATCTCGGTGTTAGGAAAGGAGTGCAGTTGCTGTATGGCTTAGATGACCTGCCTCGGCCATCGCAGATGGAGCAGCATTGTGCTAAGTGGAGACCGTACAGATCAGTGGGTTCGTGGTACATGTGGCGGCTCATTGAAGCTAAGAGCACATCAACGAGTGCTGCTGTTGCAGCCGGAGTGTCATTACCTCCATTGGAAGATATTCAACAAGAACATCAGCAGCAACAGCTTATGGACCCTCTTAATGGTGTGTTCAGTATCGGGGCTTGGGGACAATCGTAG

Protein Analysis

382

Amino Acids

41.9

Weight (kDa)

6.8

Isoelectric Point (pI)

50.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HhH-GPD PF00730 177 - 319 7.3e-22 HhH-GPD superfamily base excision DNA repair protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 273
AasI GACNNNNNNGTC 2 cut(s) 254, 496
AatII GACGTC 1 cut(s) 258
Acc36I ACCTGC 1 cut(s) 924
AccBSI CCGCTC 1 cut(s) 313
AccII CGCG 3 cut(s) 317, 446, 461
AccIII TCCGGA 1 cut(s) 231
AclWI GGATC 5 cut(s) 176, 376, 389, 443, 456
AcoI YGGCCR 1 cut(s) 923
AcsI RAATTY 3 cut(s) 568, 610, 832
AcyI GRCGYC 1 cut(s) 255
AfaI GTAC 3 cut(s) 621, 965, 986
AfiI CCNNNNNNNGG 3 cut(s) 341, 445, 1112
AflIII ACRYGT 2 cut(s) 401, 987
AgsI TTSAA 4 cut(s) 573, 751, 1004, 1073
Alw21I GWGCWC 2 cut(s) 417, 1016
Alw26I GTCTC 8 cut(s) 113, 145, 198, 246, 262, 620, 676, 952
AlwI GGATC 5 cut(s) 176, 376, 389, 443, 456
AlwNI CAGNNNCTG 1 cut(s) 898
Ama87I CYCGRG 1 cut(s) 673
Aor13HI TCCGGA 1 cut(s) 231
AoxI GGCC 1 cut(s) 923
ApeKI GCWGC 5 cut(s) 652, 940, 1028, 1037, 1087
ApoI RAATTY 3 cut(s) 568, 610, 832
Asp700I GAANNNNTTC 1 cut(s) 1068
AspS9I GGNCC 1 cut(s) 1102
AsuC2I CCSGG 1 cut(s) 336
AsuHPI GGTGA 4 cut(s) 6, 48, 275, 422
AvaI CYCGRG 1 cut(s) 673
AvaII GGWCC 1 cut(s) 1102
BamHI GGATCC 2 cut(s) 381, 448
BanII GRGCYC 1 cut(s) 417
Bbv12I GWGCWC 2 cut(s) 417, 1016
BbvCI CCTCAGC 1 cut(s) 648
BbvI GCAGC 5 cut(s) 664, 952, 1015, 1049, 1099
BccI CCATC 4 cut(s) 34, 317, 928, 934
BceAI ACGGC 1 cut(s) 184
BcnI CCSGG 1 cut(s) 336
BcoDI GTCTC 8 cut(s) 113, 145, 198, 246, 262, 620, 676, 952
BfaI CTAG 2 cut(s) 179, 708
BfuAI ACCTGC 1 cut(s) 924
BisI GCNGC 9 cut(s) 462, 601, 604, 653, 941, 995, 1029, 1038, 1088
BlsI GCNGC 9 cut(s) 463, 602, 605, 654, 942, 996, 1030, 1039, 1089
Bme1390I CCNGG 1 cut(s) 336
Bme18I GGWCC 1 cut(s) 1102
BmeT110I CYCGRG 1 cut(s) 673
BmgT120I GGNCC 1 cut(s) 1102
BmiI GGNNCC 3 cut(s) 383, 450, 1104
BmrFI CCNGG 1 cut(s) 336
BmsI GCATC 1 cut(s) 768
BplI GAGNNNNNCTC 2 cut(s) 272, 304
BpmI CTGGAG 1 cut(s) 141
Bpu10I CCTNAGC 1 cut(s) 648
BpuEI CTTGAG 1 cut(s) 282
BpuMI CCSGG 1 cut(s) 336
BsaHI GRCGYC 1 cut(s) 255
BsaI GGTCTC 1 cut(s) 952
BsaJI CCNNGG 3 cut(s) 221, 377, 920
BsaWI WCCGGW 3 cut(s) 88, 231, 260
Bsc4I CCNNNNNNNGG 3 cut(s) 341, 445, 1112
Bse118I RCCGGY 1 cut(s) 859
Bse3DI GCAATG 2 cut(s) 190, 561
BseAI TCCGGA 1 cut(s) 231
BseDI CCNNGG 3 cut(s) 221, 377, 920
BseGI GGATG 5 cut(s) 46, 328, 478, 534, 763
BseLI CCNNNNNNNGG 3 cut(s) 341, 445, 1112
BseMI GCAATG 2 cut(s) 190, 561
BseMII CTCAG 2 cut(s) 615, 662
BseRI GAGGAG 1 cut(s) 280
BseXI GCAGC 5 cut(s) 664, 952, 1015, 1049, 1099
BseYI CCCAGC 1 cut(s) 563
BsgI GTGCAG 1 cut(s) 911
Bsh1236I CGCG 3 cut(s) 317, 446, 461
BshFI GGCC 1 cut(s) 925
BsiHKAI GWGCWC 2 cut(s) 417, 1016
BsiHKCI CYCGRG 1 cut(s) 673
BsiSI CCGG 6 cut(s) 89, 232, 261, 336, 860, 1041
BslFI GGGAC 1 cut(s) 690
BslI CCNNNNNNNGG 3 cut(s) 341, 445, 1112
BsmAI GTCTC 8 cut(s) 113, 145, 198, 246, 262, 620, 676, 952
BsmBI CGTCTC 5 cut(s) 145, 198, 246, 262, 620
BsmFI GGGAC 1 cut(s) 690
BsnI GGCC 1 cut(s) 925
Bso31I GGTCTC 1 cut(s) 952
BsoBI CYCGRG 1 cut(s) 673
Bsp1286I GDGCHC 2 cut(s) 417, 1016
Bsp13I TCCGGA 1 cut(s) 231
Bsp143I GATC 5 cut(s) 168, 381, 448, 868, 968
Bsp68I TCGCGA 1 cut(s) 317
BspANI GGCC 1 cut(s) 925
BspCNI CTCAG 2 cut(s) 616, 661
BspEI TCCGGA 1 cut(s) 231
BspFNI CGCG 3 cut(s) 317, 446, 461
BspHI TCATGA 2 cut(s) 697, 825
BspLI GGNNCC 3 cut(s) 383, 450, 1104
BspMI ACCTGC 1 cut(s) 924
BspPI GGATC 5 cut(s) 176, 376, 389, 443, 456
BspTNI GGTCTC 1 cut(s) 952
BsrBI CCGCTC 1 cut(s) 313
BsrDI GCAATG 2 cut(s) 190, 561
BsrFI RCCGGY 1 cut(s) 859
BssAI RCCGGY 1 cut(s) 859
BssECI CCNNGG 3 cut(s) 221, 377, 920
BssMI GATC 5 cut(s) 168, 381, 448, 868, 968
BssNI GRCGYC 1 cut(s) 255
Bst4CI ACNGT 3 cut(s) 373, 631, 963
Bst6I CTCTTC 1 cut(s) 843
BstACI GRCGYC 1 cut(s) 255
BstC8I GCNNGC 3 cut(s) 315, 459, 552
BstDEI CTNAG 6 cut(s) 517, 624, 648, 907, 951, 1008
BstF5I GGATG 5 cut(s) 46, 328, 478, 534, 763
BstFNI CGCG 3 cut(s) 317, 446, 461
BstKTI GATC 5 cut(s) 171, 384, 451, 871, 971
BstMAI GTCTC 8 cut(s) 113, 145, 198, 246, 262, 620, 676, 952
BstMBI GATC 5 cut(s) 168, 381, 448, 868, 968
BstMWI GCNNNNNNNGC 5 cut(s) 560, 937, 1034, 1037, 1093
BstNSI RCATGY 2 cut(s) 405, 991
BstSCI CCNGG 1 cut(s) 334
BstUI CGCG 3 cut(s) 317, 446, 461
BstV1I GCAGC 5 cut(s) 664, 952, 1015, 1049, 1099
BstX2I RGATCY 2 cut(s) 381, 448
BstYI RGATCY 2 cut(s) 381, 448
BsuRI GGCC 1 cut(s) 925
BtgZI GCGATG 1 cut(s) 912
BtsCI GGATG 5 cut(s) 46, 328, 478, 534, 763
BtsIMutI CAGTG 2 cut(s) 171, 978
BtuMI TCGCGA 1 cut(s) 317
BveI ACCTGC 1 cut(s) 924
Cac8I GCNNGC 3 cut(s) 315, 459, 552
CaiI CAGNNNCTG 1 cut(s) 898
CciI TCATGA 2 cut(s) 697, 825
Cfr10I RCCGGY 1 cut(s) 859
Cfr13I GGNCC 1 cut(s) 1102
CseI GACGC 1 cut(s) 167
Csp6I GTAC 3 cut(s) 620, 964, 985
CviAII CATG 6 cut(s) 402, 698, 754, 807, 826, 988
CviQI GTAC 3 cut(s) 620, 964, 985
DdeI CTNAG 6 cut(s) 517, 624, 648, 907, 951, 1008
DpnI GATC 5 cut(s) 170, 383, 450, 870, 970
DpnII GATC 5 cut(s) 168, 381, 448, 868, 968
DrdI GACNNNNNNGTC 2 cut(s) 254, 496
DseDI GACNNNNNNGTC 2 cut(s) 254, 496
EaeI YGGCCR 1 cut(s) 923
Eam1104I CTCTTC 1 cut(s) 843
EarI CTCTTC 1 cut(s) 843
EciI GGCGGA 3 cut(s) 186, 206, 293
Ecl136II GAGCTC 1 cut(s) 415
Eco24I GRGCYC 1 cut(s) 417
Eco31I GGTCTC 1 cut(s) 952
Eco32I GATATC 1 cut(s) 286
Eco47I GGWCC 1 cut(s) 1102
Eco53kI GAGCTC 1 cut(s) 415
Eco88I CYCGRG 1 cut(s) 673
EcoICRI GAGCTC 1 cut(s) 415
EcoRI GAATTC 1 cut(s) 568
EcoRV GATATC 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 417
Esp3I CGTCTC 5 cut(s) 145, 198, 246, 262, 620
FaeI CATG 6 cut(s) 405, 701, 757, 810, 829, 991
FaqI GGGAC 1 cut(s) 690
FatI CATG 6 cut(s) 401, 697, 753, 806, 825, 987
FauNDI CATATG 1 cut(s) 819
Fnu4HI GCNGC 9 cut(s) 462, 601, 604, 653, 941, 995, 1029, 1038, 1088
FokI GGATG 5 cut(s) 33, 335, 465, 521, 770
FriOI GRGCYC 1 cut(s) 417
Fsp4HI GCNGC 9 cut(s) 462, 601, 604, 653, 941, 995, 1029, 1038, 1088
FspBI CTAG 2 cut(s) 179, 708
GluI GCNGC 9 cut(s) 462, 601, 604, 653, 941, 995, 1029, 1038, 1088
GsaI CCCAGC 1 cut(s) 567
GsuI CTGGAG 1 cut(s) 141
HaeIII GGCC 1 cut(s) 925
HapII CCGG 6 cut(s) 89, 232, 261, 336, 860, 1041
HgaI GACGC 1 cut(s) 167
Hin1I GRCGYC 1 cut(s) 255
Hin1II CATG 6 cut(s) 405, 701, 757, 810, 829, 991
HinfI GANTC 8 cut(s) 31, 92, 113, 427, 497, 508, 726, 737
HpaII CCGG 6 cut(s) 89, 232, 261, 336, 860, 1041
HphI GGTGA 4 cut(s) 6, 48, 275, 422
Hpy166II GTNNAC 2 cut(s) 304, 774
Hpy188I TCNGA 4 cut(s) 225, 399, 525, 736
Hpy188III TCNNGA 7 cut(s) 110, 232, 316, 675, 698, 775, 826
Hpy8I GTNNAC 2 cut(s) 304, 774
Hpy99I CGWCG 6 cut(s) 161, 168, 251, 322, 476, 493
HpyAV CCTTC 2 cut(s) 27, 401
HpyCH4III ACNGT 3 cut(s) 373, 631, 963
HpyCH4IV ACGT 4 cut(s) 255, 474, 491, 679
HpyCH4V TGCA 3 cut(s) 554, 892, 1037
HpyF10VI GCNNNNNNNGC 5 cut(s) 560, 937, 1034, 1037, 1093
HpyF3I CTNAG 6 cut(s) 517, 624, 648, 907, 951, 1008
HpySE526I ACGT 4 cut(s) 255, 474, 491, 679
Hsp92I GRCGYC 1 cut(s) 255
Hsp92II CATG 6 cut(s) 405, 701, 757, 810, 829, 991
Kpn2I TCCGGA 1 cut(s) 231
Kzo9I GATC 5 cut(s) 168, 381, 448, 868, 968
LmnI GCTCC 1 cut(s) 937
Lsp1109I GCAGC 5 cut(s) 664, 952, 1015, 1049, 1099
LweI GCATC 1 cut(s) 768
MaeI CTAG 2 cut(s) 179, 708
MaeII ACGT 4 cut(s) 255, 474, 491, 679
MaeIII GTNAC 1 cut(s) 440
MalI GATC 5 cut(s) 170, 383, 450, 870, 970
MbiI CCGCTC 1 cut(s) 313
MboI GATC 5 cut(s) 168, 381, 448, 868, 968
MboII GAAGA 4 cut(s) 278, 686, 830, 1076
MflI RGATCY 2 cut(s) 381, 448
MhlI GDGCHC 2 cut(s) 417, 1016
MluCI AATT 6 cut(s) 347, 568, 610, 663, 798, 832
MlyI GAGTC 3 cut(s) 25, 502, 506
MmeI TCCRAC 1 cut(s) 714
MroI TCCGGA 1 cut(s) 231
MroXI GAANNNNTTC 1 cut(s) 1068
MseI TTAA 6 cut(s) 467, 504, 641, 792, 864, 1110
MspA1I CMGCKG 1 cut(s) 119
MspI CCGG 6 cut(s) 89, 232, 261, 336, 860, 1041
MspR9I CCNGG 1 cut(s) 336
MvnI CGCG 3 cut(s) 317, 446, 461
MwoI GCNNNNNNNGC 5 cut(s) 560, 937, 1034, 1037, 1093
NciI CCSGG 1 cut(s) 336
NdeI CATATG 1 cut(s) 819
NdeII GATC 5 cut(s) 168, 381, 448, 868, 968
NlaIII CATG 6 cut(s) 405, 701, 757, 810, 829, 991
NlaIV GGNNCC 3 cut(s) 383, 450, 1104
NmeAIII GCCGAG 2 cut(s) 402, 901
NruI TCGCGA 1 cut(s) 317
NspI RCATGY 2 cut(s) 405, 991
PagI TCATGA 2 cut(s) 697, 825
PciI ACATGT 2 cut(s) 401, 987
PcsI WCGNNNNNNNCGW 3 cut(s) 197, 252, 740
PdmI GAANNNNTTC 1 cut(s) 1068
PfeI GAWTC 5 cut(s) 92, 113, 427, 726, 737
PkrI GCNGC 9 cut(s) 463, 602, 605, 654, 942, 996, 1030, 1039, 1089
PleI GAGTC 3 cut(s) 25, 502, 505
PpsI GAGTC 3 cut(s) 25, 502, 505
PscI ACATGT 2 cut(s) 401, 987
PsiI TTATAA 1 cut(s) 273
Psp124BI GAGCTC 1 cut(s) 417
PspFI CCCAGC 1 cut(s) 563
PspN4I GGNNCC 3 cut(s) 383, 450, 1104
PspPI GGNCC 1 cut(s) 1102
PstNI CAGNNNCTG 1 cut(s) 898
PsuI RGATCY 2 cut(s) 381, 448
PvuII CAGCTG 1 cut(s) 119
RruI TCGCGA 1 cut(s) 317
RsaI GTAC 3 cut(s) 621, 965, 986
RsaNI GTAC 3 cut(s) 620, 964, 985
SacI GAGCTC 1 cut(s) 417
SaqAI TTAA 6 cut(s) 467, 504, 641, 792, 864, 1110
SatI GCNGC 9 cut(s) 462, 601, 604, 653, 941, 995, 1029, 1038, 1088
Sau3AI GATC 5 cut(s) 168, 381, 448, 868, 968
Sau96I GGNCC 1 cut(s) 1102
SchI GAGTC 3 cut(s) 25, 502, 506
ScrFI CCNGG 1 cut(s) 336
SduI GDGCHC 2 cut(s) 417, 1016
SfaNI GCATC 1 cut(s) 768
SinI GGWCC 1 cut(s) 1102
SmlI CTYRAG 1 cut(s) 297
SmoI CTYRAG 1 cut(s) 297
Sse9I AATT 6 cut(s) 347, 568, 610, 663, 798, 832
SspMI CTAG 2 cut(s) 179, 708
SstI GAGCTC 1 cut(s) 417
StyD4I CCNGG 1 cut(s) 334
TaaI ACNGT 3 cut(s) 373, 631, 963
TaiI ACGT 4 cut(s) 258, 477, 494, 682
TaqI TCGA 8 cut(s) 29, 111, 156, 163, 246, 417, 471, 844
TasI AATT 6 cut(s) 347, 568, 610, 663, 798, 832
TauI GCSGC 4 cut(s) 464, 603, 606, 997
TfiI GAWTC 5 cut(s) 92, 113, 427, 726, 737
Tru1I TTAA 6 cut(s) 467, 504, 641, 792, 864, 1110
Tru9I TTAA 6 cut(s) 467, 504, 641, 792, 864, 1110
TscAI CASTG 2 cut(s) 178, 978
TseI GCWGC 5 cut(s) 652, 940, 1028, 1037, 1087
TspDTI ATGAA 7 cut(s) 117, 467, 572, 686, 806, 814, 830
TspGWI ACGGA 6 cut(s) 181, 264, 335, 428, 501, 738
TspRI CASTG 2 cut(s) 178, 978
VpaK11BI GGWCC 1 cut(s) 1102
XapI RAATTY 3 cut(s) 568, 610, 832
XceI RCATGY 2 cut(s) 405, 991
XmnI GAANNNNTTC 1 cut(s) 1068
XspI CTAG 2 cut(s) 179, 708
ZraI GACGTC 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.