RchiOBHm_Chr6g0301151

DNA-3-methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0007076
Physical Location & Seq
Forward (+)
61681101 .. 61686446
5346 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27046

Sequence Viewer

Length: 1116 bp
ATGGGAGAGCAAACCCAGCCCCAGACTCAGGCTCAGACCGAACCACCTCAGTCTGAGACTCCTCAACCGCCGCCGCAGCATGACTCTATCGCCACCACCACAATCGCCGAACCAACTCCGACCGAACTCACCAATGCTCCGCCCCTAACCTCCTCTCCCCCTTCCAAAATCCCCTTCCGCCCGCGGAAGATCCGGAAGCTCTCGCCCGACAGTTCAGACCCGAATCCATCTCAACAAACCGCCGCCGCGCCCGAAAACCCCAAGCCCAGGCCCACAAAGTCCAAGATCGTCCAGCCACGCGCCGTCTCGGTTCCGAGAGCCGTCGGCCGACCTCTCCGGTGCGAAGGCGAGGTCGAATCGGCGATTCGGCATCTCCGGAACGCCGATCCGCTTCTGATTCCGTTGATCGACGCCCACCAACCACCGCAATTCGACAACTTCCACACTCCGTTCCTTGCCCTAACCCGTAGCATTCTCTACCAGCAACTCGCCTACAAGGCCGGCACGTCGATCTACACGCGCTTCATCCAGCTCTGCGGCGGCGAGGCCTGCGTCAATCCTGAGAGCGTGCTCGCCCAGAGCGCTATACAGCTCCGGCAAATCGGAATCTCCGGGCGGAAAGCCAGCTACCTCCATGACTTGGCCAGGAAGTACCAGAATGGAATTCTCTCTGATGCTGCCATTGTAAATATGGACGACAAGTCGTTGTTCACAATGCTGACCATGGTCAGTGGCATTGGCTCTTGGTCTGTCCACATGTTCATGATTTTCTCGCTGCACCGGCCCGATGTGCTTCCGGTGAATGACCTAGGGATTCGCAAAGGTGTTCAGCTCCTCTACGGGCTTGAGGAGTTGCCTCGGCCCTCACAGATGGAGACGCTGTGTGACAAGTGGCGGCCTTATAGGTCAGTCGCAGCTTGGTATCTGTGGAGGTATGTTGAATCCAAGGGAGCTTCTTCTACGGCGGCAGCGGTTGCCGCAGGTGCTATAGCACCAATGCAGCAGCAACAGGAGGAGGAACAACAATCGCAGCACCCGCAGCATCCTCAGCAACAGCAGCTTATGGATTCGCTCAGCAGCCTAATTAATATTGGGGCCTGTACCTGGGGACCTTGA

Protein Analysis

371

Amino Acids

40.96

Weight (kDa)

8.24

Isoelectric Point (pI)

53.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HhH-GPD PF00730 156 - 299 4.2e-18 HhH-GPD superfamily base excision DNA repair protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 971
Acc36I ACCTGC 1 cut(s) 971
AccB7I CCANNNNNTGG 1 cut(s) 640
AccII CGCG 4 cut(s) 184, 248, 300, 520
AccIII TCCGGA 2 cut(s) 192, 375
AclWI GGATC 2 cut(s) 184, 380
AcoI YGGCCR 2 cut(s) 325, 642
AcsI RAATTY 1 cut(s) 663
AcyI GRCGYC 1 cut(s) 411
AfaI GTAC 2 cut(s) 653, 1102
AfeI AGCGCT 1 cut(s) 583
AfiI CCNNNNNNNGG 4 cut(s) 28, 267, 640, 1104
AflIII ACRYGT 1 cut(s) 756
AgsI TTSAA 1 cut(s) 941
AhdI GACNNNNNGTC 1 cut(s) 700
AjiI CACGTC 1 cut(s) 507
AjnI CCWGG 3 cut(s) 266, 644, 1103
AjuI GAANNNNNNNTTGG 2 cut(s) 158, 190
AluBI AGCT 8 cut(s) 199, 532, 592, 627, 832, 917, 953, 1060
AluI AGCT 8 cut(s) 199, 532, 592, 627, 832, 917, 953, 1060
Alw21I GWGCWC 1 cut(s) 573
Alw26I GTCTC 3 cut(s) 50, 310, 869
AlwI GGATC 2 cut(s) 184, 380
Aor13HI TCCGGA 2 cut(s) 192, 375
Aor51HI AGCGCT 1 cut(s) 583
AoxI GGCC 9 cut(s) 269, 325, 498, 546, 642, 782, 860, 896, 1095
ApoI RAATTY 1 cut(s) 663
ArsI GACNNNNNNTTYG 2 cut(s) 336, 368
AseI ATTAAT 1 cut(s) 1086
AspA2I CCTAGG 1 cut(s) 808
AspLEI GCGC 4 cut(s) 250, 302, 522, 584
AspS9I GGNCC 5 cut(s) 270, 783, 861, 1095, 1109
AsuC2I CCSGG 1 cut(s) 613
AsuHPI GGTGA 2 cut(s) 121, 811
AvaII GGWCC 1 cut(s) 1109
AvrII CCTAGG 1 cut(s) 808
BalI TGGCCA 1 cut(s) 644
Bbv12I GWGCWC 1 cut(s) 573
BbvCI CCTCAGC 1 cut(s) 1047
BccI CCATC 2 cut(s) 235, 865
BceAI ACGGC 3 cut(s) 287, 305, 978
BciT130I CCWGG 3 cut(s) 268, 646, 1105
BcnI CCSGG 1 cut(s) 613
BcoDI GTCTC 3 cut(s) 50, 310, 869
BfaI CTAG 1 cut(s) 809
BfmI CTRYAG 1 cut(s) 987
BfoI RGCGCY 1 cut(s) 585
BfuAI ACCTGC 1 cut(s) 971
BglI GCCNNNNNGGC 1 cut(s) 497
BlnI CCTAGG 1 cut(s) 808
BlpI GCTNAGC 1 cut(s) 1073
Bme1390I CCNGG 4 cut(s) 268, 613, 646, 1105
Bme18I GGWCC 1 cut(s) 1109
BmeRI GACNNNNNGTC 1 cut(s) 700
BmgBI CACGTC 1 cut(s) 507
BmgT120I GGNCC 5 cut(s) 270, 783, 861, 1095, 1109
BmiI GGNNCC 3 cut(s) 312, 1096, 1110
BmrFI CCNGG 4 cut(s) 268, 613, 646, 1105
BmsI GCATC 3 cut(s) 379, 664, 1051
BoxI GACNNNNGTC 1 cut(s) 725
Bpu10I CCTNAGC 1 cut(s) 1047
Bpu1102I GCTNAGC 1 cut(s) 1073
BpuEI CTTGAG 1 cut(s) 866
BpuMI CCSGG 1 cut(s) 613
BsaHI GRCGYC 1 cut(s) 411
BsaJI CCNNGG 7 cut(s) 182, 266, 723, 808, 857, 945, 1104
BsaWI WCCGGW 4 cut(s) 192, 336, 375, 796
BsaXI ACNNNNNCTCC 6 cut(s) 121, 139, 151, 169, 866, 896
Bsc4I CCNNNNNNNGG 4 cut(s) 28, 267, 640, 1104
Bse118I RCCGGY 2 cut(s) 500, 780
BseAI TCCGGA 2 cut(s) 192, 375
BseBI CCWGG 3 cut(s) 268, 646, 1105
BseDI CCNNGG 7 cut(s) 182, 266, 723, 808, 857, 945, 1104
BseGI GGATG 2 cut(s) 525, 1042
BseLI CCNNNNNNNGG 4 cut(s) 28, 267, 640, 1104
BseMII CTCAG 7 cut(s) 41, 45, 47, 62, 552, 1061, 1087
BseRI GAGGAG 5 cut(s) 51, 142, 824, 863, 1028
BseX3I CGGCCG 1 cut(s) 325
BseYI CCCAGC 1 cut(s) 15
BsgI GTGCAG 1 cut(s) 761
Bsh1236I CGCG 4 cut(s) 184, 248, 300, 520
Bsh1285I CGRYCG 2 cut(s) 123, 328
BshFI GGCC 9 cut(s) 271, 327, 500, 548, 644, 784, 862, 898, 1097
BsiEI CGRYCG 2 cut(s) 123, 328
BsiHKAI GWGCWC 1 cut(s) 573
BsiSI CCGG 8 cut(s) 193, 337, 376, 501, 595, 612, 781, 797
BslI CCNNNNNNNGG 4 cut(s) 28, 267, 640, 1104
BsmAI GTCTC 3 cut(s) 50, 310, 869
BsmBI CGTCTC 2 cut(s) 310, 869
BsmI GAATGC 1 cut(s) 471
BsnI GGCC 9 cut(s) 271, 327, 500, 548, 644, 784, 862, 898, 1097
Bsp1286I GDGCHC 1 cut(s) 573
Bsp13I TCCGGA 2 cut(s) 192, 375
Bsp143I GATC 5 cut(s) 189, 285, 385, 405, 510
Bsp1720I GCTNAGC 1 cut(s) 1073
Bsp19I CCATGG 1 cut(s) 723
BspANI GGCC 9 cut(s) 271, 327, 500, 548, 644, 784, 862, 898, 1097
BspCNI CTCAG 7 cut(s) 40, 46, 46, 61, 553, 1060, 1086
BspEI TCCGGA 2 cut(s) 192, 375
BspFNI CGCG 4 cut(s) 184, 248, 300, 520
BspHI TCATGA 1 cut(s) 762
BspLI GGNNCC 3 cut(s) 312, 1096, 1110
BspMI ACCTGC 1 cut(s) 971
BspPI GGATC 2 cut(s) 184, 380
BsrFI RCCGGY 2 cut(s) 500, 780
BssAI RCCGGY 2 cut(s) 500, 780
BssECI CCNNGG 7 cut(s) 182, 266, 723, 808, 857, 945, 1104
BssMI GATC 5 cut(s) 189, 285, 385, 405, 510
BssNI GRCGYC 1 cut(s) 411
BssT1I CCWWGG 3 cut(s) 723, 808, 945
Bst2UI CCWGG 3 cut(s) 268, 646, 1105
Bst4CI ACNGT 1 cut(s) 212
BstACI GRCGYC 1 cut(s) 411
BstAPI GCANNNNNTGC 1 cut(s) 974
BstC8I GCNNGC 6 cut(s) 182, 502, 550, 569, 573, 625
BstDEI CTNAG 7 cut(s) 27, 33, 48, 54, 561, 1047, 1073
BstDSI CCRYGG 2 cut(s) 182, 723
BstF5I GGATG 2 cut(s) 525, 1042
BstFNI CGCG 4 cut(s) 184, 248, 300, 520
BstH2I RGCGCY 1 cut(s) 585
BstHHI GCGC 4 cut(s) 250, 302, 522, 584
BstKTI GATC 5 cut(s) 192, 288, 388, 408, 513
BstMAI GTCTC 3 cut(s) 50, 310, 869
BstMBI GATC 5 cut(s) 189, 285, 385, 405, 510
BstMCI CGRYCG 2 cut(s) 123, 328
BstNI CCWGG 3 cut(s) 268, 646, 1105
BstNSI RCATGY 1 cut(s) 760
BstPAI GACNNNNGTC 1 cut(s) 725
BstSCI CCNGG 4 cut(s) 266, 611, 644, 1103
BstSFI CTRYAG 1 cut(s) 987
BstUI CGCG 4 cut(s) 184, 248, 300, 520
BstX2I RGATCY 1 cut(s) 189
BstYI RGATCY 1 cut(s) 189
BstZI CGGCCG 1 cut(s) 325
BsuRI GGCC 9 cut(s) 271, 327, 500, 548, 644, 784, 862, 898, 1097
BtgI CCRYGG 2 cut(s) 182, 723
BtrI CACGTC 1 cut(s) 507
BtsCI GGATG 2 cut(s) 525, 1042
BtsIMutI CAGTG 1 cut(s) 736
BveI ACCTGC 1 cut(s) 971
Cac8I GCNNGC 6 cut(s) 182, 502, 550, 569, 573, 625
CciI TCATGA 1 cut(s) 762
CfoI GCGC 4 cut(s) 250, 302, 522, 584
Cfr10I RCCGGY 2 cut(s) 500, 780
Cfr13I GGNCC 5 cut(s) 270, 783, 861, 1095, 1109
Cfr42I CCGCGG 1 cut(s) 185
CseI GACGC 3 cut(s) 419, 541, 886
Csp6I GTAC 2 cut(s) 652, 1101
CviAII CATG 5 cut(s) 80, 635, 724, 757, 763
CviQI GTAC 2 cut(s) 652, 1101
DdeI CTNAG 7 cut(s) 27, 33, 48, 54, 561, 1047, 1073
DpnI GATC 5 cut(s) 191, 287, 387, 407, 512
DpnII GATC 5 cut(s) 189, 285, 385, 405, 510
DriI GACNNNNNGTC 1 cut(s) 700
EaeI YGGCCR 2 cut(s) 325, 642
EagI CGGCCG 1 cut(s) 325
Eam1105I GACNNNNNGTC 1 cut(s) 700
EciI GGCGGA 3 cut(s) 129, 167, 631
EclXI CGGCCG 1 cut(s) 325
Eco130I CCWWGG 3 cut(s) 723, 808, 945
Eco147I AGGCCT 1 cut(s) 548
Eco47I GGWCC 1 cut(s) 1109
Eco47III AGCGCT 1 cut(s) 583
Eco52I CGGCCG 1 cut(s) 325
EcoO109I RGGNCCY 2 cut(s) 1095, 1109
EcoRI GAATTC 1 cut(s) 663
EcoRII CCWGG 3 cut(s) 266, 644, 1103
EcoT14I CCWWGG 3 cut(s) 723, 808, 945
ErhI CCWWGG 3 cut(s) 723, 808, 945
Esp3I CGTCTC 2 cut(s) 310, 869
FaeI CATG 5 cut(s) 83, 638, 727, 760, 766
FatI CATG 5 cut(s) 79, 634, 723, 756, 762
FauI CCCGC 2 cut(s) 189, 1044
FokI GGATG 2 cut(s) 512, 1029
FspBI CTAG 1 cut(s) 809
GlaI GCGC 4 cut(s) 249, 301, 521, 583
GsaI CCCAGC 1 cut(s) 19
HaeII RGCGCY 1 cut(s) 585
HaeIII GGCC 9 cut(s) 271, 327, 500, 548, 644, 784, 862, 898, 1097
HapII CCGG 8 cut(s) 193, 337, 376, 501, 595, 612, 781, 797
HgaI GACGC 3 cut(s) 419, 541, 886
HhaI GCGC 4 cut(s) 250, 302, 522, 584
Hin1I GRCGYC 1 cut(s) 411
Hin1II CATG 5 cut(s) 83, 638, 727, 760, 766
Hin6I GCGC 4 cut(s) 248, 300, 520, 582
HinP1I GCGC 4 cut(s) 248, 300, 520, 582
HpaII CCGG 8 cut(s) 193, 337, 376, 501, 595, 612, 781, 797
HphI GGTGA 2 cut(s) 121, 811
Hpy166II GTNNAC 2 cut(s) 711, 754
Hpy188I TCNGA 8 cut(s) 36, 55, 120, 217, 315, 396, 605, 673
Hpy188III TCNNGA 4 cut(s) 193, 376, 560, 763
Hpy8I GTNNAC 2 cut(s) 711, 754
Hpy99I CGWCG 3 cut(s) 326, 413, 511
HpyAV CCTTC 3 cut(s) 171, 184, 338
HpyCH4III ACNGT 1 cut(s) 212
HpyCH4IV ACGT 1 cut(s) 506
HpyCH4V TGCA 2 cut(s) 778, 1000
HpyF3I CTNAG 7 cut(s) 27, 33, 48, 54, 561, 1047, 1073
HpySE526I ACGT 1 cut(s) 506
Hsp92I GRCGYC 1 cut(s) 411
Hsp92II CATG 5 cut(s) 83, 638, 727, 760, 766
HspAI GCGC 4 cut(s) 248, 300, 520, 582
Kpn2I TCCGGA 2 cut(s) 192, 375
KroI GCCGGC 1 cut(s) 500
KroNI GCCGGC 1 cut(s) 502
KspI CCGCGG 1 cut(s) 185
Kzo9I GATC 5 cut(s) 189, 285, 385, 405, 510
LmnI GCTCC 4 cut(s) 142, 597, 837, 950
LweI GCATC 3 cut(s) 379, 664, 1051
MaeI CTAG 1 cut(s) 809
MaeII ACGT 1 cut(s) 506
MaeIII GTNAC 1 cut(s) 884
MalI GATC 5 cut(s) 191, 287, 387, 407, 512
MboI GATC 5 cut(s) 189, 285, 385, 405, 510
MboII GAAGA 2 cut(s) 199, 948
MflI RGATCY 1 cut(s) 189
MhlI GDGCHC 1 cut(s) 573
MlsI TGGCCA 1 cut(s) 644
MluCI AATT 3 cut(s) 428, 663, 1083
MluNI TGGCCA 1 cut(s) 644
MlyI GAGTC 3 cut(s) 19, 52, 77
MmeI TCCRAC 1 cut(s) 143
Mox20I TGGCCA 1 cut(s) 644
MroI TCCGGA 2 cut(s) 192, 375
MroNI GCCGGC 1 cut(s) 500
MscI TGGCCA 1 cut(s) 644
MseI TTAA 1 cut(s) 1086
MslI CAYNNNNRTG 1 cut(s) 761
Msp20I TGGCCA 1 cut(s) 644
MspA1I CMGCKG 2 cut(s) 184, 971
MspI CCGG 8 cut(s) 193, 337, 376, 501, 595, 612, 781, 797
MspR9I CCNGG 4 cut(s) 268, 613, 646, 1105
Mva1269I GAATGC 1 cut(s) 471
MvaI CCWGG 3 cut(s) 268, 646, 1105
MvnI CGCG 4 cut(s) 184, 248, 300, 520
NaeI GCCGGC 1 cut(s) 502
NciI CCSGG 1 cut(s) 613
NcoI CCATGG 1 cut(s) 723
NdeII GATC 5 cut(s) 189, 285, 385, 405, 510
NgoMIV GCCGGC 1 cut(s) 500
NlaIII CATG 5 cut(s) 83, 638, 727, 760, 766
NlaIV GGNNCC 3 cut(s) 312, 1096, 1110
NmeAIII GCCGAG 1 cut(s) 838
NmuCI GTSAC 1 cut(s) 884
NspI RCATGY 1 cut(s) 760
PagI TCATGA 1 cut(s) 762
PaqCI CACCTGC 1 cut(s) 971
PceI AGGCCT 1 cut(s) 548
PciI ACATGT 1 cut(s) 756
PctI GAATGC 1 cut(s) 471
PdiI GCCGGC 1 cut(s) 502
PfeI GAWTC 8 cut(s) 223, 356, 364, 397, 606, 814, 941, 1067
PflMI CCANNNNNTGG 1 cut(s) 640
PleI GAGTC 3 cut(s) 19, 52, 77
PpsI GAGTC 3 cut(s) 19, 52, 77
PpuMI RGGWCCY 1 cut(s) 1109
PscI ACATGT 1 cut(s) 756
PshAI GACNNNNGTC 1 cut(s) 725
PshBI ATTAAT 1 cut(s) 1086
Psp5II RGGWCCY 1 cut(s) 1109
Psp6I CCWGG 3 cut(s) 266, 644, 1103
PspFI CCCAGC 1 cut(s) 15
PspGI CCWGG 3 cut(s) 266, 644, 1103
PspN4I GGNNCC 3 cut(s) 312, 1096, 1110
PspPI GGNCC 5 cut(s) 270, 783, 861, 1095, 1109
PspPPI RGGWCCY 1 cut(s) 1109
PsuI RGATCY 1 cut(s) 189
RsaI GTAC 2 cut(s) 653, 1102
RsaNI GTAC 2 cut(s) 652, 1101
RseI CAYNNNNRTG 1 cut(s) 761
SacII CCGCGG 1 cut(s) 185
SaqAI TTAA 1 cut(s) 1086
Sau3AI GATC 5 cut(s) 189, 285, 385, 405, 510
Sau96I GGNCC 5 cut(s) 270, 783, 861, 1095, 1109
SchI GAGTC 3 cut(s) 19, 52, 77
ScrFI CCNGG 4 cut(s) 268, 613, 646, 1105
SduI GDGCHC 1 cut(s) 573
SfaNI GCATC 3 cut(s) 379, 664, 1051
SfcI CTRYAG 1 cut(s) 987
Sfr303I CCGCGG 1 cut(s) 185
SgrBI CCGCGG 1 cut(s) 185
SinI GGWCC 1 cut(s) 1109
SmiMI CAYNNNNRTG 1 cut(s) 761
SmlI CTYRAG 1 cut(s) 845
SmoI CTYRAG 1 cut(s) 845
Sse9I AATT 3 cut(s) 428, 663, 1083
SseBI AGGCCT 1 cut(s) 548
SspI AATATT 1 cut(s) 1090
SspMI CTAG 1 cut(s) 809
StuI AGGCCT 1 cut(s) 548
StyD4I CCNGG 4 cut(s) 266, 611, 644, 1103
StyI CCWWGG 3 cut(s) 723, 808, 945
TaaI ACNGT 1 cut(s) 212
TaiI ACGT 1 cut(s) 509
TaqI TCGA 4 cut(s) 354, 408, 432, 509
TaqII GACCGA 2 cut(s) 53, 137
TasI AATT 3 cut(s) 428, 663, 1083
TauI GCSGC 9 cut(s) 73, 76, 245, 248, 540, 543, 898, 968, 980
TfiI GAWTC 8 cut(s) 223, 356, 364, 397, 606, 814, 941, 1067
Tru1I TTAA 1 cut(s) 1086
Tru9I TTAA 1 cut(s) 1086
TscAI CASTG 1 cut(s) 736
TseFI GTSAC 1 cut(s) 884
Tsp45I GTSAC 1 cut(s) 884
TspDTI ATGAA 2 cut(s) 514, 751
TspGWI ACGGA 2 cut(s) 390, 438
TspRI CASTG 1 cut(s) 736
Van91I CCANNNNNTGG 1 cut(s) 640
VpaK11BI GGWCC 1 cut(s) 1109
VspI ATTAAT 1 cut(s) 1086
XapI RAATTY 1 cut(s) 663
XceI RCATGY 1 cut(s) 760
XcmI CCANNNNNNNNNTGG 1 cut(s) 688
XmaJI CCTAGG 1 cut(s) 808
XspI CTAG 1 cut(s) 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.