FvH4_2g39710

DNA-3-methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0007076
Physical Location & Seq
Reverse (-)
28439836 .. 28444330
4495 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g39710.t1

Sequence Viewer

Length: 1158 bp
ATGGGAGAGAAAACTCAGGTCCAAGCCCCATCCCAGCCTCAGACTCCGACTCCGACTCCGACTCAGACCGAACCTCAGTCTGAGACTCCTCAACCGCCTCCGCCGCCGCCGCCGCACCATGACACCATCGCCGCCACCACAATCTCCGAACCGACTCCCACCGAACTCACCAATGCTCCGCCCCTAACCTCCTCTCCCCCTTCCAAAATCCCCTTCCGCCCCCGCAAGATCCGGAAGCTCTCCCCCGACGGCTCCGACCCGAAACCATCCAAAACCGCCATCGTCGCCGCCGCCACCGCGCCTGAAAAGCCCAAGCTCAGGCCCGCCAAGTGCAAGATCGTCCAGACACGCGCCGTCTCGGTTCCCCGAGCCGTCGGCCGGCCTCTCCGCTGCGACGGCGAGGTCGAATCGGCGATTCGGCATCTCCGGAACGCCGATCCGCTTCTGATTCCGTTAATCGAGGCCCACCAGCCACCGCAGTTCGACAACTTCCACACTCCCTTCCTTGCCCTAACCCGAAGCATTCTCTACCAGCAACTCGCTTACAAGGCCGGCACGTCGATCTACACGCGCTTCATCCAGCTCTGCGGCGGCGAGTCCGCCGTGAATCCCGAGACCGTGCTCGCACAGAGCGCTACTCAGCTCCGGCAAATCGGAATCTCCGGCCGGAAAGCCAGCTACCTCCATGACTTAGCTAGAAAATACCAGAATGGAATTCTATCCGATACTGCCATTGTAAATATGGACGACAAGTCGTTGTTCACTATGCTCACCATGGTCAGTGGCATTGGCTCTTGGTCTGTGCACATGTTCATGATTTTCTCGTTGCACCGCCCCGACGTGCTTCCGGTGAATGATCTTGGGATTCGGAAAGGTGTGCAGCTCCTCTACGGGCTTGAGGAGCTGCCTCGGCCCTCGCATATGGATCAGCTGTGTGACAAGTGGCGGCCGTATAGGTCCGTGGCTGCTTGGTATCTGTGGAGGTATGTTGAGTCCAAGGGAGCTTCTTCTACGGCCGCAGCGGTTGCGGCAGGTGCTATAGCACCAATGCAGCAGCAGCAGGAGGACCAACAACCGCAGCAGCATCCTCAGCAACAGCAGCTTATGGATTCGCTCAGCAACCTAATTAATATTGGGGCATGTACCTGGGGACAGTGA

Protein Analysis

386

Amino Acids

42.32

Weight (kDa)

9.13

Isoelectric Point (pI)

48.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HhH-GPD PF00730 173 - 316 1.1e-18 HhH-GPD superfamily base excision DNA repair protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1022
AasI GACNNNNNNGTC 1 cut(s) 401
Acc36I ACCTGC 1 cut(s) 1022
AccII CGCG 3 cut(s) 299, 351, 571
AccIII TCCGGA 2 cut(s) 231, 426
AclWI GGATC 3 cut(s) 223, 431, 933
AcoI YGGCCR 4 cut(s) 376, 664, 947, 1014
AcsI RAATTY 1 cut(s) 714
AfaI GTAC 1 cut(s) 1144
AfeI AGCGCT 1 cut(s) 634
AfiI CCNNNNNNNGG 2 cut(s) 318, 378
AflIII ACRYGT 1 cut(s) 807
AhdI GACNNNNNGTC 1 cut(s) 751
AjiI CACGTC 2 cut(s) 558, 841
AjnI CCWGG 1 cut(s) 1145
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
Alw21I GWGCWC 2 cut(s) 624, 807
Alw26I GTCTC 3 cut(s) 77, 361, 608
Alw44I GTGCAC 1 cut(s) 803
AlwI GGATC 3 cut(s) 223, 431, 933
Ama87I CYCGRG 2 cut(s) 366, 611
Aor13HI TCCGGA 2 cut(s) 231, 426
Aor51HI AGCGCT 1 cut(s) 634
AoxI GGCC 9 cut(s) 320, 376, 380, 462, 549, 664, 911, 947, 1014
ApaLI GTGCAC 1 cut(s) 803
ApoI RAATTY 1 cut(s) 714
AseI ATTAAT 1 cut(s) 1128
AspLEI GCGC 4 cut(s) 301, 353, 573, 635
AspS9I GGNCC 6 cut(s) 19, 321, 463, 912, 957, 1066
AsuHPI GGTGA 3 cut(s) 160, 763, 862
AvaI CYCGRG 2 cut(s) 366, 611
AvaII GGWCC 3 cut(s) 19, 957, 1066
BaeGI GKGCMC 1 cut(s) 807
Bbv12I GWGCWC 2 cut(s) 624, 807
BbvCI CCTCAGC 1 cut(s) 1089
BccI CCATC 4 cut(s) 37, 134, 274, 287
BceAI ACGGC 7 cut(s) 265, 338, 356, 412, 587, 934, 1029
BciT130I CCWGG 1 cut(s) 1147
BcoDI GTCTC 3 cut(s) 77, 361, 608
BfaI CTAG 1 cut(s) 696
BfmI CTRYAG 1 cut(s) 1038
BfoI RGCGCY 1 cut(s) 636
BfuAI ACCTGC 1 cut(s) 1022
BlpI GCTNAGC 1 cut(s) 1115
Bme1390I CCNGG 1 cut(s) 1147
Bme18I GGWCC 3 cut(s) 19, 957, 1066
BmeRI GACNNNNNGTC 1 cut(s) 751
BmeT110I CYCGRG 2 cut(s) 366, 611
BmgBI CACGTC 2 cut(s) 558, 841
BmgT120I GGNCC 6 cut(s) 19, 321, 463, 912, 957, 1066
BmiI GGNNCC 2 cut(s) 253, 363
BmrFI CCNGG 1 cut(s) 1147
BmsI GCATC 2 cut(s) 430, 1093
BplI GAGNNNNNCTC 2 cut(s) 622, 654
Bpu10I CCTNAGC 2 cut(s) 317, 1089
Bpu1102I GCTNAGC 1 cut(s) 1115
BpuEI CTTGAG 1 cut(s) 917
BsaI GGTCTC 1 cut(s) 608
BsaJI CCNNGG 5 cut(s) 774, 908, 960, 996, 1146
BsaWI WCCGGW 3 cut(s) 231, 426, 847
BsaXI ACNNNNNCTCC 8 cut(s) 34, 40, 64, 70, 160, 178, 190, 208
Bsc4I CCNNNNNNNGG 2 cut(s) 318, 378
Bse118I RCCGGY 2 cut(s) 378, 551
BseAI TCCGGA 2 cut(s) 231, 426
BseBI CCWGG 1 cut(s) 1147
BseDI CCNNGG 5 cut(s) 774, 908, 960, 996, 1146
BseGI GGATG 4 cut(s) 29, 266, 576, 1084
BseLI CCNNNNNNNGG 2 cut(s) 318, 378
BseMII CTCAG 9 cut(s) 29, 53, 72, 77, 89, 331, 653, 1103, 1129
BseRI GAGGAG 4 cut(s) 78, 181, 875, 914
BseSI GKGCMC 1 cut(s) 807
BseX3I CGGCCG 4 cut(s) 376, 664, 947, 1014
BseYI CCCAGC 1 cut(s) 33
BsgI GTGCAG 1 cut(s) 899
Bsh1236I CGCG 3 cut(s) 299, 351, 571
Bsh1285I CGRYCG 4 cut(s) 379, 667, 950, 1017
BshFI GGCC 9 cut(s) 322, 378, 382, 464, 551, 666, 913, 949, 1016
BsiEI CGRYCG 4 cut(s) 379, 667, 950, 1017
BsiHKAI GWGCWC 2 cut(s) 624, 807
BsiHKCI CYCGRG 2 cut(s) 366, 611
BsiSI CCGG 8 cut(s) 232, 379, 427, 552, 646, 663, 667, 848
BslI CCNNNNNNNGG 2 cut(s) 318, 378
BsmAI GTCTC 3 cut(s) 77, 361, 608
BsmBI CGTCTC 1 cut(s) 361
BsmI GAATGC 1 cut(s) 522
BsnI GGCC 9 cut(s) 322, 378, 382, 464, 551, 666, 913, 949, 1016
Bso31I GGTCTC 1 cut(s) 608
BsoBI CYCGRG 2 cut(s) 366, 611
Bsp1286I GDGCHC 2 cut(s) 624, 807
Bsp13I TCCGGA 2 cut(s) 231, 426
Bsp143I GATC 6 cut(s) 228, 336, 436, 561, 856, 925
Bsp1720I GCTNAGC 1 cut(s) 1115
Bsp19I CCATGG 1 cut(s) 774
BspANI GGCC 9 cut(s) 322, 378, 382, 464, 551, 666, 913, 949, 1016
BspCNI CTCAG 9 cut(s) 28, 52, 73, 76, 88, 330, 652, 1102, 1128
BspEI TCCGGA 2 cut(s) 231, 426
BspFNI CGCG 3 cut(s) 299, 351, 571
BspHI TCATGA 1 cut(s) 813
BspLI GGNNCC 2 cut(s) 253, 363
BspMI ACCTGC 1 cut(s) 1022
BspPI GGATC 3 cut(s) 223, 431, 933
BspTNI GGTCTC 1 cut(s) 608
BsrFI RCCGGY 2 cut(s) 378, 551
BssAI RCCGGY 2 cut(s) 378, 551
BssECI CCNNGG 5 cut(s) 774, 908, 960, 996, 1146
BssMI GATC 6 cut(s) 228, 336, 436, 561, 856, 925
BssT1I CCWWGG 2 cut(s) 774, 996
Bst2UI CCWGG 1 cut(s) 1147
Bst4CI ACNGT 2 cut(s) 619, 1155
BstAPI GCANNNNNTGC 1 cut(s) 1025
BstC8I GCNNGC 5 cut(s) 324, 380, 553, 624, 676
BstDSI CCRYGG 2 cut(s) 774, 960
BstF5I GGATG 4 cut(s) 29, 266, 576, 1084
BstFNI CGCG 3 cut(s) 299, 351, 571
BstH2I RGCGCY 1 cut(s) 636
BstHHI GCGC 4 cut(s) 301, 353, 573, 635
BstKTI GATC 6 cut(s) 231, 339, 439, 564, 859, 928
BstMAI GTCTC 3 cut(s) 77, 361, 608
BstMBI GATC 6 cut(s) 228, 336, 436, 561, 856, 925
BstMCI CGRYCG 4 cut(s) 379, 667, 950, 1017
BstNI CCWGG 1 cut(s) 1147
BstNSI RCATGY 2 cut(s) 811, 1143
BstSCI CCNGG 1 cut(s) 1145
BstSFI CTRYAG 1 cut(s) 1038
BstSLI GKGCMC 1 cut(s) 807
BstUI CGCG 3 cut(s) 299, 351, 571
BstX2I RGATCY 1 cut(s) 228
BstYI RGATCY 1 cut(s) 228
BstZI CGGCCG 4 cut(s) 376, 664, 947, 1014
BsuRI GGCC 9 cut(s) 322, 378, 382, 464, 551, 666, 913, 949, 1016
BtgI CCRYGG 2 cut(s) 774, 960
BtgZI GCGATG 1 cut(s) 112
BtrI CACGTC 2 cut(s) 558, 841
BtsCI GGATG 4 cut(s) 29, 266, 576, 1084
BtsIMutI CAGTG 1 cut(s) 787
BveI ACCTGC 1 cut(s) 1022
Cac8I GCNNGC 5 cut(s) 324, 380, 553, 624, 676
CciI TCATGA 1 cut(s) 813
CfoI GCGC 4 cut(s) 301, 353, 573, 635
Cfr10I RCCGGY 2 cut(s) 378, 551
Cfr13I GGNCC 6 cut(s) 19, 321, 463, 912, 957, 1066
Csp6I GTAC 1 cut(s) 1143
CviAII CATG 6 cut(s) 119, 686, 775, 808, 814, 1140
CviQI GTAC 1 cut(s) 1143
DpnI GATC 6 cut(s) 230, 338, 438, 563, 858, 927
DpnII GATC 6 cut(s) 228, 336, 436, 561, 856, 925
DrdI GACNNNNNNGTC 1 cut(s) 401
DriI GACNNNNNGTC 1 cut(s) 751
DseDI GACNNNNNNGTC 1 cut(s) 401
EaeI YGGCCR 4 cut(s) 376, 664, 947, 1014
EagI CGGCCG 4 cut(s) 376, 664, 947, 1014
Eam1105I GACNNNNNGTC 1 cut(s) 751
EciI GGCGGA 4 cut(s) 90, 168, 206, 589
EclXI CGGCCG 4 cut(s) 376, 664, 947, 1014
Eco130I CCWWGG 2 cut(s) 774, 996
Eco31I GGTCTC 1 cut(s) 608
Eco47I GGWCC 3 cut(s) 19, 957, 1066
Eco47III AGCGCT 1 cut(s) 634
Eco52I CGGCCG 4 cut(s) 376, 664, 947, 1014
Eco88I CYCGRG 2 cut(s) 366, 611
EcoRI GAATTC 1 cut(s) 714
EcoRII CCWGG 1 cut(s) 1145
EcoT14I CCWWGG 2 cut(s) 774, 996
ErhI CCWWGG 2 cut(s) 774, 996
Esp3I CGTCTC 1 cut(s) 361
FaeI CATG 6 cut(s) 122, 689, 778, 811, 817, 1143
FatI CATG 6 cut(s) 118, 685, 774, 807, 813, 1139
FauI CCCGC 2 cut(s) 230, 331
FauNDI CATATG 1 cut(s) 921
FokI GGATG 4 cut(s) 16, 253, 563, 1071
FseI GGCCGGCC 1 cut(s) 382
FspBI CTAG 1 cut(s) 696
GlaI GCGC 4 cut(s) 300, 352, 572, 634
GsaI CCCAGC 1 cut(s) 37
HaeII RGCGCY 1 cut(s) 636
HaeIII GGCC 9 cut(s) 322, 378, 382, 464, 551, 666, 913, 949, 1016
HapII CCGG 8 cut(s) 232, 379, 427, 552, 646, 663, 667, 848
HhaI GCGC 4 cut(s) 301, 353, 573, 635
Hin1II CATG 6 cut(s) 122, 689, 778, 811, 817, 1143
Hin6I GCGC 4 cut(s) 299, 351, 571, 633
HinP1I GCGC 4 cut(s) 299, 351, 571, 633
HpaII CCGG 8 cut(s) 232, 379, 427, 552, 646, 663, 667, 848
HphI GGTGA 3 cut(s) 160, 763, 862
Hpy166II GTNNAC 2 cut(s) 762, 805
Hpy188III TCNNGA 5 cut(s) 232, 343, 427, 611, 814
Hpy8I GTNNAC 2 cut(s) 762, 805
Hpy99I CGWCG 6 cut(s) 251, 287, 377, 398, 562, 842
HpyAV CCTTC 3 cut(s) 210, 223, 511
HpyCH4III ACNGT 2 cut(s) 619, 1155
HpyCH4IV ACGT 2 cut(s) 557, 840
HpyCH4V TGCA 5 cut(s) 333, 805, 829, 880, 1051
HpySE526I ACGT 2 cut(s) 557, 840
Hsp92II CATG 6 cut(s) 122, 689, 778, 811, 817, 1143
HspAI GCGC 4 cut(s) 299, 351, 571, 633
Kpn2I TCCGGA 2 cut(s) 231, 426
KroI GCCGGC 2 cut(s) 378, 551
KroNI GCCGGC 2 cut(s) 380, 553
Kzo9I GATC 6 cut(s) 228, 336, 436, 561, 856, 925
LmnI GCTCC 6 cut(s) 181, 257, 648, 888, 901, 1001
LweI GCATC 2 cut(s) 430, 1093
MaeI CTAG 1 cut(s) 696
MaeII ACGT 2 cut(s) 557, 840
MaeIII GTNAC 1 cut(s) 935
MalI GATC 6 cut(s) 230, 338, 438, 563, 858, 927
MboI GATC 6 cut(s) 228, 336, 436, 561, 856, 925
MboII GAAGA 1 cut(s) 999
MflI RGATCY 1 cut(s) 228
MhlI GDGCHC 2 cut(s) 624, 807
MluCI AATT 2 cut(s) 714, 1125
MlyI GAGTC 8 cut(s) 37, 43, 49, 55, 79, 148, 605, 1001
MmeI TCCRAC 4 cut(s) 71, 77, 83, 279
MroI TCCGGA 2 cut(s) 231, 426
MroNI GCCGGC 2 cut(s) 378, 551
MseI TTAA 2 cut(s) 455, 1128
MslI CAYNNNNRTG 1 cut(s) 812
MspA1I CMGCKG 3 cut(s) 390, 931, 1022
MspI CCGG 8 cut(s) 232, 379, 427, 552, 646, 663, 667, 848
MspR9I CCNGG 1 cut(s) 1147
Mva1269I GAATGC 1 cut(s) 522
MvaI CCWGG 1 cut(s) 1147
MvnI CGCG 3 cut(s) 299, 351, 571
NaeI GCCGGC 2 cut(s) 380, 553
NcoI CCATGG 1 cut(s) 774
NdeI CATATG 1 cut(s) 921
NdeII GATC 6 cut(s) 228, 336, 436, 561, 856, 925
NgoMIV GCCGGC 2 cut(s) 378, 551
NlaIII CATG 6 cut(s) 122, 689, 778, 811, 817, 1143
NlaIV GGNNCC 2 cut(s) 253, 363
NmeAIII GCCGAG 1 cut(s) 889
NmuCI GTSAC 1 cut(s) 935
NspI RCATGY 2 cut(s) 811, 1143
PagI TCATGA 1 cut(s) 813
PaqCI CACCTGC 1 cut(s) 1022
PciI ACATGT 1 cut(s) 807
PcsI WCGNNNNNNNCGW 1 cut(s) 402
PctI GAATGC 1 cut(s) 522
PdiI GCCGGC 2 cut(s) 380, 553
PfeI GAWTC 7 cut(s) 407, 415, 448, 607, 657, 865, 1109
PleI GAGTC 8 cut(s) 37, 43, 49, 55, 79, 148, 604, 1000
PpsI GAGTC 8 cut(s) 37, 43, 49, 55, 79, 148, 604, 1000
PscI ACATGT 1 cut(s) 807
PshBI ATTAAT 1 cut(s) 1128
Psp6I CCWGG 1 cut(s) 1145
PspFI CCCAGC 1 cut(s) 33
PspGI CCWGG 1 cut(s) 1145
PspN4I GGNNCC 2 cut(s) 253, 363
PspPI GGNCC 6 cut(s) 19, 321, 463, 912, 957, 1066
PsuI RGATCY 1 cut(s) 228
PvuII CAGCTG 1 cut(s) 931
RigI GGCCGGCC 1 cut(s) 382
RsaI GTAC 1 cut(s) 1144
RsaNI GTAC 1 cut(s) 1143
RseI CAYNNNNRTG 1 cut(s) 812
SaqAI TTAA 2 cut(s) 455, 1128
Sau3AI GATC 6 cut(s) 228, 336, 436, 561, 856, 925
Sau96I GGNCC 6 cut(s) 19, 321, 463, 912, 957, 1066
SchI GAGTC 8 cut(s) 37, 43, 49, 55, 79, 148, 605, 1001
ScrFI CCNGG 1 cut(s) 1147
SduI GDGCHC 2 cut(s) 624, 807
SfaNI GCATC 2 cut(s) 430, 1093
SfcI CTRYAG 1 cut(s) 1038
SinI GGWCC 3 cut(s) 19, 957, 1066
SmiMI CAYNNNNRTG 1 cut(s) 812
SmlI CTYRAG 1 cut(s) 896
SmoI CTYRAG 1 cut(s) 896
Sse9I AATT 2 cut(s) 714, 1125
SspI AATATT 1 cut(s) 1132
SspMI CTAG 1 cut(s) 696
StyD4I CCNGG 1 cut(s) 1145
StyI CCWWGG 2 cut(s) 774, 996
TaaI ACNGT 2 cut(s) 619, 1155
TaiI ACGT 2 cut(s) 560, 843
TaqI TCGA 4 cut(s) 405, 459, 483, 560
TaqII GACCGA 1 cut(s) 83
TasI AATT 2 cut(s) 714, 1125
TfiI GAWTC 7 cut(s) 407, 415, 448, 607, 657, 865, 1109
Tru1I TTAA 2 cut(s) 455, 1128
Tru9I TTAA 2 cut(s) 455, 1128
TscAI CASTG 1 cut(s) 787
TseFI GTSAC 1 cut(s) 935
Tsp45I GTSAC 1 cut(s) 935
TspDTI ATGAA 2 cut(s) 565, 802
TspGWI ACGGA 2 cut(s) 441, 949
TspRI CASTG 1 cut(s) 787
VneI GTGCAC 1 cut(s) 803
VpaK11BI GGWCC 3 cut(s) 19, 957, 1066
VspI ATTAAT 1 cut(s) 1128
XapI RAATTY 1 cut(s) 714
XceI RCATGY 2 cut(s) 811, 1143
XcmI CCANNNNNNNNNTGG 1 cut(s) 739
XspI CTAG 1 cut(s) 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.