MD15G1032400.v1.1

helicase MAGATAMA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0028370
Physical Location & Seq
Reverse (-)
2002348 .. 2003093
746 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1032400.v1.1.491

Sequence Viewer

Length: 705 bp
ATGCATCCGGAGATCATCAGGAGCTTTCCTTCTGCTGAGTTTTATGCAGAGTCATTAGAGGATGGTCCTCATATCGGAAGACCGGACGGACTTTCATGGCATGACTACTGTTGTTTCGGACCCTTCTGTTTTTTTTTTTTTTTACTTGGGAAGCAAGAATCCGGAAATATATCTTTGGTGAATGTTGTGAGATATCAAGAGCTGAAATCAAGTCATAAGTTGGCGGTTATATACCAGTATCGTGCACAAGCAGATCTCTTAAAACAGCGTTTCAGAAGTGATTTCAGAGGTGGGTTTGAGAAACAAGTGGATGTTACTACTATGGATGGTTACCAGGGACTGAAAAAGATGTTGCCATATTTTCTTGTGTTCGTGCAAATGATAAAAAAGGACCGCAACATTGGATTTGTGGGTCATTTTCGGCGGATTATTGTTGAGATCACTAGGGCAAAATCTTCTATCCTGGTAGTGCAATCTGTATTAACACTGAGGAAGGACAGGCATTGGAGTAACCTAGTGGAAAGTGCTAAAGAAAATAGATTGTTTGAGGTTACGGACTATGATAAATTTTTTAGTGATGAGAATCTGAAATCGCTTGCTGTCAAGAAAGAATCCTCGTTGTCAATGGAGGAAGTTTGCGATGACAACGTGGATAACCCCGTGCCTTTCACCAACTTCCGGACGGTGACGAAGCTCAGGATATGA

Protein Analysis

235

Amino Acids

27.26

Weight (kDa)

8.58

Isoelectric Point (pI)

46.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_12 PF13087 1 - 157 2e-17 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028370)

Species Orthologous Gene IDs
malus_domestica MD15G1032400.v1.1
pyrus_communis pycom15g02810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 3 cut(s) 7, 161, 678
AciI CCGC 3 cut(s) 224, 394, 424
AcsI RAATTY 1 cut(s) 566
AfiI CCNNNNNNNGG 2 cut(s) 74, 678
AjnI CCWGG 2 cut(s) 333, 462
AluBI AGCT 3 cut(s) 24, 202, 694
AluI AGCT 3 cut(s) 24, 202, 694
Alw21I GWGCWC 1 cut(s) 247
Alw44I GTGCAC 1 cut(s) 243
AlwNI CAGNNNCTG 1 cut(s) 340
Aor13HI TCCGGA 3 cut(s) 7, 161, 678
ApaLI GTGCAC 1 cut(s) 243
ApoI RAATTY 1 cut(s) 566
AspS9I GGNCC 3 cut(s) 65, 119, 391
AsuHPI GGTGA 3 cut(s) 190, 661, 697
AvaII GGWCC 3 cut(s) 65, 119, 391
BaeGI GKGCMC 1 cut(s) 247
BbsI GAAGAC 1 cut(s) 85
Bbv12I GWGCWC 1 cut(s) 247
BccI CCATC 2 cut(s) 56, 320
BciT130I CCWGG 2 cut(s) 335, 464
BfaI CTAG 2 cut(s) 444, 515
BglII AGATCT 1 cut(s) 253
Bme1390I CCNGG 2 cut(s) 335, 464
Bme18I GGWCC 3 cut(s) 65, 119, 391
BmgT120I GGNCC 3 cut(s) 65, 119, 391
BmiI GGNNCC 1 cut(s) 121
BmrFI CCNGG 2 cut(s) 335, 464
BmsI GCATC 1 cut(s) 13
BpiI GAAGAC 1 cut(s) 85
Bpu10I CCTNAGC 1 cut(s) 695
BsaBI GATNNNNATC 1 cut(s) 582
BsaJI CCNNGG 1 cut(s) 334
BsaWI WCCGGW 4 cut(s) 7, 82, 161, 678
Bsc4I CCNNNNNNNGG 2 cut(s) 74, 678
Bse1I ACTGG 1 cut(s) 235
Bse8I GATNNNNATC 1 cut(s) 582
BseAI TCCGGA 3 cut(s) 7, 161, 678
BseBI CCWGG 2 cut(s) 335, 464
BseDI CCNNGG 1 cut(s) 334
BseGI GGATG 4 cut(s) 4, 67, 316, 331
BseJI GATNNNNATC 1 cut(s) 582
BseLI CCNNNNNNNGG 2 cut(s) 74, 678
BseMII CTCAG 2 cut(s) 27, 479
BseNI ACTGG 1 cut(s) 235
BseSI GKGCMC 1 cut(s) 247
BsiHKAI GWGCWC 1 cut(s) 247
BsiSI CCGG 4 cut(s) 8, 83, 162, 679
BslFI GGGAC 1 cut(s) 351
BslI CCNNNNNNNGG 2 cut(s) 74, 678
BsmFI GGGAC 1 cut(s) 351
Bsp1286I GDGCHC 1 cut(s) 247
Bsp13I TCCGGA 3 cut(s) 7, 161, 678
Bsp143I GATC 3 cut(s) 12, 253, 438
BspACI CCGC 3 cut(s) 224, 394, 424
BspCNI CTCAG 2 cut(s) 28, 480
BspEI TCCGGA 3 cut(s) 7, 161, 678
BspLI GGNNCC 1 cut(s) 121
BsrI ACTGG 1 cut(s) 235
BssECI CCNNGG 1 cut(s) 334
BssMI GATC 3 cut(s) 12, 253, 438
Bst2UI CCWGG 2 cut(s) 335, 464
Bst4CI ACNGT 2 cut(s) 110, 685
BstC8I GCNNGC 1 cut(s) 597
BstDEI CTNAG 3 cut(s) 36, 488, 695
BstEII GGTNACC 1 cut(s) 329
BstF5I GGATG 4 cut(s) 4, 67, 316, 331
BstKTI GATC 3 cut(s) 15, 256, 441
BstMBI GATC 3 cut(s) 12, 253, 438
BstNI CCWGG 2 cut(s) 335, 464
BstPI GGTNACC 1 cut(s) 329
BstSCI CCNGG 2 cut(s) 333, 462
BstSLI GKGCMC 1 cut(s) 247
BstV2I GAAGAC 1 cut(s) 85
BstX2I RGATCY 1 cut(s) 253
BstYI RGATCY 1 cut(s) 253
BtgZI GCGATG 1 cut(s) 654
BtsCI GGATG 4 cut(s) 4, 67, 316, 331
BtsIMutI CAGTG 1 cut(s) 485
Cac8I GCNNGC 1 cut(s) 597
CaiI CAGNNNCTG 1 cut(s) 340
Cfr13I GGNCC 3 cut(s) 65, 119, 391
CviAII CATG 2 cut(s) 96, 101
CviJI RGCY 3 cut(s) 24, 202, 694
CviKI_1 RGCY 3 cut(s) 24, 202, 694
DdeI CTNAG 3 cut(s) 36, 488, 695
DpnI GATC 3 cut(s) 14, 255, 440
DpnII GATC 3 cut(s) 12, 253, 438
EciI GGCGGA 1 cut(s) 439
Eco32I GATATC 1 cut(s) 194
Eco47I GGWCC 3 cut(s) 65, 119, 391
Eco91I GGTNACC 1 cut(s) 329
EcoO65I GGTNACC 1 cut(s) 329
EcoRII CCWGG 2 cut(s) 333, 462
EcoRV GATATC 1 cut(s) 194
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 2 cut(s) 99, 104
FaqI GGGAC 1 cut(s) 351
FatI CATG 2 cut(s) 95, 100
FokI GGATG 3 cut(s) 74, 323, 338
FspBI CTAG 2 cut(s) 444, 515
HapII CCGG 4 cut(s) 8, 83, 162, 679
Hin1II CATG 2 cut(s) 99, 104
HinfI GANTC 4 cut(s) 50, 158, 583, 611
HpaII CCGG 4 cut(s) 8, 83, 162, 679
HphI GGTGA 3 cut(s) 190, 661, 697
Hpy166II GTNNAC 1 cut(s) 245
Hpy188I TCNGA 5 cut(s) 77, 119, 275, 287, 588
Hpy188III TCNNGA 7 cut(s) 8, 19, 162, 197, 604, 679, 697
Hpy8I GTNNAC 1 cut(s) 245
HpyAV CCTTC 3 cut(s) 39, 133, 487
HpyCH4III ACNGT 2 cut(s) 110, 685
HpyCH4IV ACGT 1 cut(s) 648
HpyCH4V TGCA 5 cut(s) 4, 47, 245, 376, 472
HpyF3I CTNAG 3 cut(s) 36, 488, 695
HpySE526I ACGT 1 cut(s) 648
Hsp92II CATG 2 cut(s) 99, 104
Kpn2I TCCGGA 3 cut(s) 7, 161, 678
Kzo9I GATC 3 cut(s) 12, 253, 438
LmnI GCTCC 1 cut(s) 21
LweI GCATC 1 cut(s) 13
MaeI CTAG 2 cut(s) 444, 515
MaeII ACGT 1 cut(s) 648
MaeIII GTNAC 5 cut(s) 313, 329, 509, 550, 685
MalI GATC 3 cut(s) 14, 255, 440
MboI GATC 3 cut(s) 12, 253, 438
MboII GAAGA 2 cut(s) 90, 447
MflI RGATCY 1 cut(s) 253
MhlI GDGCHC 1 cut(s) 247
MluCI AATT 1 cut(s) 566
MlyI GAGTC 1 cut(s) 59
MnlI CCTC 7 cut(s) 52, 78, 281, 483, 541, 622, 625
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 3 cut(s) 7, 161, 678
MseI TTAA 2 cut(s) 260, 482
MspI CCGG 4 cut(s) 8, 83, 162, 679
MspR9I CCNGG 2 cut(s) 335, 464
MvaI CCWGG 2 cut(s) 335, 464
NdeII GATC 3 cut(s) 12, 253, 438
NlaIII CATG 2 cut(s) 99, 104
NlaIV GGNNCC 1 cut(s) 121
NmuCI GTSAC 1 cut(s) 685
NsiI ATGCAT 1 cut(s) 6
PfeI GAWTC 3 cut(s) 158, 583, 611
PleI GAGTC 1 cut(s) 58
PpsI GAGTC 1 cut(s) 58
Psp6I CCWGG 2 cut(s) 333, 462
PspEI GGTNACC 1 cut(s) 329
PspGI CCWGG 2 cut(s) 333, 462
PspN4I GGNNCC 1 cut(s) 121
PspPI GGNCC 3 cut(s) 65, 119, 391
PstNI CAGNNNCTG 1 cut(s) 340
PsuI RGATCY 1 cut(s) 253
SaqAI TTAA 2 cut(s) 260, 482
Sau3AI GATC 3 cut(s) 12, 253, 438
Sau96I GGNCC 3 cut(s) 65, 119, 391
SchI GAGTC 1 cut(s) 59
ScrFI CCNGG 2 cut(s) 335, 464
SduI GDGCHC 1 cut(s) 247
SetI ASST 7 cut(s) 26, 204, 292, 516, 552, 651, 696
SfaNI GCATC 1 cut(s) 13
SinI GGWCC 3 cut(s) 65, 119, 391
Sse9I AATT 1 cut(s) 566
SsiI CCGC 3 cut(s) 224, 394, 424
SspMI CTAG 2 cut(s) 444, 515
StyD4I CCNGG 2 cut(s) 333, 462
TaaI ACNGT 2 cut(s) 110, 685
TaiI ACGT 1 cut(s) 651
TasI AATT 1 cut(s) 566
TfiI GAWTC 3 cut(s) 158, 583, 611
Tru1I TTAA 2 cut(s) 260, 482
Tru9I TTAA 2 cut(s) 260, 482
TscAI CASTG 1 cut(s) 492
TseFI GTSAC 1 cut(s) 685
Tsp45I GTSAC 1 cut(s) 685
TspDTI ATGAA 1 cut(s) 84
TspGWI ACGGA 2 cut(s) 102, 569
TspRI CASTG 1 cut(s) 492
VneI GTGCAC 1 cut(s) 243
VpaK11BI GGWCC 3 cut(s) 65, 119, 391
XapI RAATTY 1 cut(s) 566
XspI CTAG 2 cut(s) 444, 515
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.