MD15G1091400.v1.1

Lachrymatory-factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0024287
Physical Location & Seq
Forward (+)
6351844 .. 6352982
1139 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1091400.v1.1.491

Sequence Viewer

Length: 417 bp
ATGGGAATAAGGAAGAATTTTTCTATTGAGATTGTTGAAAACCAATCACCATTAAAATGGGAAGGCAAAACCGCTTGTGAGCTCATAGTCCATGCAGCAGAACAAGTTTGGCCTCTTTGGGCCGATTTTTGTAGCTTGCACAAATGGTTGCCAATCCTTGACACACGTCAATTTCTACATCCATACGGATCTCTAACTTCGTCCCTGATTGATGGAGTCCTCGGCCAGCCATGTGTGATCCGGTACTGCGCCGCTTCTCTTCCTTCGCCGGACGATGGGACAACCATCAAGTGGGCCAAAGAAAAGCTCCTAATGATTGATCCAATCAACCATTATTTAAGCTATGAGATCAATGAAAACAATATCGGGTTCATGCCATACGTTGCAAGTTGCAACCATGCAATGAGTCCTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

139

Amino Acids

15.62

Weight (kDa)

5.9

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc2 PF10604 32 - 130 1.9e-06 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024287)

Species Orthologous Gene IDs
malus_domestica MD15G1091400.v1.1 MD15G1091500.v1.1
pyrus_communis pycom15g08540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 291
AciI CCGC 2 cut(s) 72, 252
AclWI GGATC 3 cut(s) 196, 232, 314
AcoI YGGCCR 1 cut(s) 223
AcsI RAATTY 1 cut(s) 16
AfaI GTAC 1 cut(s) 245
AfiI CCNNNNNNNGG 2 cut(s) 275, 291
AflIII ACRYGT 1 cut(s) 164
AgsI TTSAA 1 cut(s) 38
AjiI CACGTC 1 cut(s) 167
AluBI AGCT 4 cut(s) 82, 135, 307, 342
AluI AGCT 4 cut(s) 82, 135, 307, 342
Alw21I GWGCWC 1 cut(s) 84
AlwI GGATC 3 cut(s) 196, 232, 314
AoxI GGCC 4 cut(s) 110, 120, 223, 294
ApeKI GCWGC 1 cut(s) 95
ApoI RAATTY 1 cut(s) 16
AspLEI GCGC 1 cut(s) 251
AspS9I GGNCC 2 cut(s) 120, 294
AsuHPI GGTGA 1 cut(s) 39
BanII GRGCYC 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 84
BbvI GCAGC 1 cut(s) 107
BccI CCATC 3 cut(s) 206, 269, 293
BisI GCNGC 2 cut(s) 96, 252
BlsI GCNGC 2 cut(s) 97, 253
BmgBI CACGTC 1 cut(s) 167
BmgT120I GGNCC 2 cut(s) 120, 294
BoxI GACNNNNGTC 1 cut(s) 165
BsaJI CCNNGG 1 cut(s) 220
BsaWI WCCGGW 1 cut(s) 240
Bsc4I CCNNNNNNNGG 2 cut(s) 275, 291
Bse3DI GCAATG 1 cut(s) 408
BseDI CCNNGG 1 cut(s) 220
BseGI GGATG 1 cut(s) 178
BseLI CCNNNNNNNGG 2 cut(s) 275, 291
BseMI GCAATG 1 cut(s) 408
BseXI GCAGC 1 cut(s) 107
BshFI GGCC 4 cut(s) 112, 122, 225, 296
BsiHKAI GWGCWC 1 cut(s) 84
BsiSI CCGG 2 cut(s) 241, 269
BslFI GGGAC 2 cut(s) 187, 292
BslI CCNNNNNNNGG 2 cut(s) 275, 291
BsmFI GGGAC 2 cut(s) 187, 292
BsnI GGCC 4 cut(s) 112, 122, 225, 296
Bsp1286I GDGCHC 1 cut(s) 84
Bsp143I GATC 4 cut(s) 188, 237, 319, 348
BspACI CCGC 2 cut(s) 72, 252
BspANI GGCC 4 cut(s) 112, 122, 225, 296
BspPI GGATC 3 cut(s) 196, 232, 314
BsrDI GCAATG 1 cut(s) 408
BssECI CCNNGG 1 cut(s) 220
BssMI GATC 4 cut(s) 188, 237, 319, 348
Bst6I CTCTTC 1 cut(s) 264
BstC8I GCNNGC 2 cut(s) 137, 227
BstF5I GGATG 1 cut(s) 178
BstHHI GCGC 1 cut(s) 251
BstKTI GATC 4 cut(s) 191, 240, 322, 351
BstMBI GATC 4 cut(s) 188, 237, 319, 348
BstPAI GACNNNNGTC 1 cut(s) 165
BstV1I GCAGC 1 cut(s) 107
BstX2I RGATCY 1 cut(s) 188
BstXI CCANNNNNNTGG 1 cut(s) 57
BstYI RGATCY 1 cut(s) 188
BsuRI GGCC 4 cut(s) 112, 122, 225, 296
BtrI CACGTC 1 cut(s) 167
BtsCI GGATG 1 cut(s) 178
Cac8I GCNNGC 2 cut(s) 137, 227
CfoI GCGC 1 cut(s) 251
Cfr13I GGNCC 2 cut(s) 120, 294
Csp6I GTAC 1 cut(s) 244
CviAII CATG 4 cut(s) 92, 231, 373, 398
CviJI RGCY 9 cut(s) 82, 112, 122, 135, 225, 229, 296, 307, 342
CviKI_1 RGCY 9 cut(s) 82, 112, 122, 135, 225, 229, 296, 307, 342
CviQI GTAC 1 cut(s) 244
DpnI GATC 4 cut(s) 190, 239, 321, 350
DpnII GATC 4 cut(s) 188, 237, 319, 348
EaeI YGGCCR 1 cut(s) 223
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
Ecl136II GAGCTC 1 cut(s) 82
Eco24I GRGCYC 1 cut(s) 84
Eco53kI GAGCTC 1 cut(s) 82
EcoICRI GAGCTC 1 cut(s) 82
EcoT38I GRGCYC 1 cut(s) 84
FaeI CATG 4 cut(s) 95, 234, 376, 401
FaiI YATR 8 cut(s) 86, 93, 184, 232, 345, 374, 379, 399
FaqI GGGAC 2 cut(s) 187, 292
FatI CATG 4 cut(s) 91, 230, 372, 397
Fnu4HI GCNGC 2 cut(s) 96, 252
FokI GGATG 1 cut(s) 165
FriOI GRGCYC 1 cut(s) 84
Fsp4HI GCNGC 2 cut(s) 96, 252
GlaI GCGC 1 cut(s) 250
GluI GCNGC 2 cut(s) 96, 252
HaeIII GGCC 4 cut(s) 112, 122, 225, 296
HapII CCGG 2 cut(s) 241, 269
HhaI GCGC 1 cut(s) 251
Hin1II CATG 4 cut(s) 95, 234, 376, 401
Hin6I GCGC 1 cut(s) 249
HinP1I GCGC 1 cut(s) 249
HinfI GANTC 2 cut(s) 216, 406
HpaII CCGG 2 cut(s) 241, 269
HphI GGTGA 1 cut(s) 39
HpyAV CCTTC 2 cut(s) 56, 273
HpyCH4IV ACGT 2 cut(s) 166, 381
HpyCH4V TGCA 5 cut(s) 95, 139, 386, 393, 401
HpySE526I ACGT 2 cut(s) 166, 381
Hsp92II CATG 4 cut(s) 95, 234, 376, 401
HspAI GCGC 1 cut(s) 249
Kzo9I GATC 4 cut(s) 188, 237, 319, 348
LmnI GCTCC 1 cut(s) 312
LpnPI CCDG 4 cut(s) 218, 239, 254, 282
Lsp1109I GCAGC 1 cut(s) 107
MaeII ACGT 2 cut(s) 166, 381
MalI GATC 4 cut(s) 190, 239, 321, 350
MboI GATC 4 cut(s) 188, 237, 319, 348
MboII GAAGA 2 cut(s) 25, 251
MflI RGATCY 1 cut(s) 188
MhlI GDGCHC 1 cut(s) 84
MluCI AATT 2 cut(s) 16, 170
MlyI GAGTC 2 cut(s) 225, 415
MnlI CCTC 2 cut(s) 123, 230
MseI TTAA 2 cut(s) 53, 338
MslI CAYNNNNRTG 1 cut(s) 55
MspI CCGG 2 cut(s) 241, 269
NdeII GATC 4 cut(s) 188, 237, 319, 348
NlaIII CATG 4 cut(s) 95, 234, 376, 401
NmeAIII GCCGAG 1 cut(s) 201
PflMI CCANNNNNTGG 1 cut(s) 291
PkrI GCNGC 2 cut(s) 97, 253
PleI GAGTC 2 cut(s) 224, 414
PpsI GAGTC 2 cut(s) 224, 414
PshAI GACNNNNGTC 1 cut(s) 165
Psp124BI GAGCTC 1 cut(s) 84
PspPI GGNCC 2 cut(s) 120, 294
PsuI RGATCY 1 cut(s) 188
RsaI GTAC 1 cut(s) 245
RsaNI GTAC 1 cut(s) 244
RseI CAYNNNNRTG 1 cut(s) 55
SacI GAGCTC 1 cut(s) 84
SaqAI TTAA 2 cut(s) 53, 338
SatI GCNGC 2 cut(s) 96, 252
Sau3AI GATC 4 cut(s) 188, 237, 319, 348
Sau96I GGNCC 2 cut(s) 120, 294
SchI GAGTC 2 cut(s) 225, 415
SduI GDGCHC 1 cut(s) 84
SetI ASST 6 cut(s) 84, 137, 169, 309, 344, 384
SmiMI CAYNNNNRTG 1 cut(s) 55
Sse9I AATT 2 cut(s) 16, 170
SsiI CCGC 2 cut(s) 72, 252
SstI GAGCTC 1 cut(s) 84
TaiI ACGT 2 cut(s) 169, 384
TasI AATT 2 cut(s) 16, 170
TauI GCSGC 1 cut(s) 254
Tru1I TTAA 2 cut(s) 53, 338
Tru9I TTAA 2 cut(s) 53, 338
TseI GCWGC 1 cut(s) 95
TspDTI ATGAA 2 cut(s) 361, 369
TspGWI ACGGA 1 cut(s) 201
Van91I CCANNNNNTGG 1 cut(s) 291
XapI RAATTY 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.