MD15G1120700.v1.1

Peptidyl-prolyl cis-trans isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0028426
Physical Location & Seq
Forward (+)
8681404 .. 8683311
1908 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1120700.v1.1.491

Sequence Viewer

Length: 1245 bp
ATGAGTATCAGTCCCAGGAGGAGTCCGACCAAGAGCCCAAGTTTAAGTCACAAACGGAGTGAGAGCAGGAGTTCGAGTGTTAGTAGAAGCCCTCCTCCCCCTCAGAGGAGCAGGAGCATCAGCAGGAGTCCTGTTAGAAGCGATAGCAGTAGAAGCCCAGTCAGGGGTTTCAACAGAAGTCCAATGAGGGGCTGGAAGGGTAGGAGTCCAGTGAAAGGCCGGTCTCAAAGAAGCATAAGCAGGAGTCCTGTAAGCAGGAGCCCACCATATAGCAGAAGCCCGGTAAAAGTTCCAAGAAGCGTAAGCCGAAGTCCACCAAGAAAATCATTGCGCAAGTCAATTAGTAGAAGCCCTGTTGGAGCTTCAAGAAGCAGAAGCCCAGTCATAGCTTCTCGAAGGAGAGCAAGCAGAAGCCCTGTAAGAGTCTCAAGAAGCAGAAGCCCAGTTAGATCTTATCGAAGGAGTGCAAGCAGAAGCCCTGTAAGAGTTTCAAGAAGCAGAAGCCCAGTTAGATCTTCTCGAAGGAGTGCAAGCAGAAGTTCAGGTAGGGTTCCTTCTAGGAAAACTGCTAGCCGCAGTCCATTGAGGGCCCCAAGCAGGAATAACCGTCGCAGCTATTCAAGGAGTCCTAGCCCAGTAGCGCGGAGGGTAAGGTCGCCTATATCACCAGATCGGGGGAGGAGCTTATCAAGAAGTGCTTCACCTGATGGCTCACCAAAGCGCATTAGAAGAGGGCGGGGTTTCAGTCAGCGGTACTCTTATGCACGGAGGTACAGAACCCCTTCTTTGTCTCCTGTGAGATCTTATCGTTATGGTGGTGGTCGAAGTGATCGTGACAGATATTCAAGTTATAGAAGGTCGAGGTACTCTCCAAGGCGCTATAGAAGCCCACCAAGAAGAACACCTCCTAGATACAGAAGCAGAAGAAGCAGAAGTCCTGTATCACGTAGCCCCCGTTATCGCGGCCGAAACTATAGTAGGAGCCGTAGCCCTGTCCGAAGTCGTAGCCCCATCCAGAGCCGCAGTCGTTCTCCAGTGGAGGTGTCTAGATCACGTGTCTCTCCTCGGGTTGAGAGGGCAAGATCTCCATCCAGAAGCAGGAGCCCATCAAGATCAAGATCAAGATCAAGGTCCTCTCTGGATTCCAAATCTCCAATTCGGACCGGAAAAAGCAGGTCTAGGTCATCATCAGGAAGCCCAATAGGGAAGAAGGGGTTGGTTTCGTACGATGATGTTTCCCCCTAG

Protein Analysis

415

Amino Acids

46.91

Weight (kDa)

12.0

Isoelectric Point (pI)

176.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028426)

Species Orthologous Gene IDs
malus_domestica MD15G1120700.v1.1
pyrus_communis pycom15g10890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 332
Acc36I ACCTGC 1 cut(s) 1164
AccII CGCG 2 cut(s) 643, 963
AciI CCGC 6 cut(s) 574, 643, 736, 751, 963, 1021
AcoI YGGCCR 1 cut(s) 964
AcvI CACGTG 1 cut(s) 1055
AfaI GTAC 4 cut(s) 755, 773, 866, 1226
AfiI CCNNNNNNNGG 8 cut(s) 105, 163, 164, 188, 215, 597, 674, 1098
AflIII ACRYGT 1 cut(s) 1054
AgsI TTSAA 5 cut(s) 172, 366, 492, 621, 846
AjnI CCWGG 1 cut(s) 14
AjuI GAANNNNNNNTTGG 2 cut(s) 1199, 1231
AluBI AGCT 4 cut(s) 362, 389, 615, 684
AluI AGCT 4 cut(s) 362, 389, 615, 684
Alw26I GTCTC 4 cut(s) 228, 430, 795, 1063
Ama87I CYCGRG 1 cut(s) 1065
AoxI GGCC 3 cut(s) 217, 588, 964
ApaI GGGCCC 1 cut(s) 592
ApeKI GCWGC 1 cut(s) 612
Asp700I GAANNNNTTC 2 cut(s) 697, 781
AspLEI GCGC 4 cut(s) 333, 643, 723, 879
AspS9I GGNCC 4 cut(s) 588, 589, 1131, 1161
AsuC2I CCSGG 1 cut(s) 281
AsuHPI GGTGA 3 cut(s) 657, 693, 705
AsuNHI GCTAGC 1 cut(s) 569
AvaI CYCGRG 1 cut(s) 1065
AvaII GGWCC 2 cut(s) 1131, 1161
BaeGI GKGCMC 1 cut(s) 592
BanII GRGCYC 4 cut(s) 38, 263, 592, 1106
BarI GAAGNNNNNNTAC 2 cut(s) 538, 570
BbrPI CACGTG 1 cut(s) 1055
BbvI GCAGC 1 cut(s) 624
BccI CCATC 4 cut(s) 701, 1019, 1096, 1114
BceAI ACGGC 1 cut(s) 969
BciT130I CCWGG 1 cut(s) 16
BcnI CCSGG 1 cut(s) 281
BcoDI GTCTC 4 cut(s) 228, 430, 795, 1063
BfaI CTAG 7 cut(s) 558, 570, 630, 909, 1047, 1179, 1243
BfmI CTRYAG 2 cut(s) 880, 973
BfoI RGCGCY 1 cut(s) 880
BfuAI ACCTGC 1 cut(s) 1164
BglII AGATCT 4 cut(s) 449, 512, 800, 1082
BisI GCNGC 4 cut(s) 574, 613, 964, 1021
BlsI GCNGC 4 cut(s) 575, 614, 965, 1022
Bme1390I CCNGG 2 cut(s) 16, 281
Bme18I GGWCC 2 cut(s) 1131, 1161
BmeT110I CYCGRG 1 cut(s) 1065
BmgT120I GGNCC 4 cut(s) 588, 589, 1131, 1161
BmiI GGNNCC 6 cut(s) 260, 552, 590, 591, 983, 1103
BmrFI CCNGG 2 cut(s) 16, 281
BmrI ACTGGG 5 cut(s) 152, 374, 437, 500, 629
BmsI GCATC 1 cut(s) 126
BmtI GCTAGC 1 cut(s) 573
BmuI ACTGGG 5 cut(s) 152, 374, 437, 500, 629
BplI GAGNNNNNCTC 2 cut(s) 853, 885
BpmI CTGGAG 1 cut(s) 1017
BpuEI CTTGAG 1 cut(s) 412
BpuMI CCSGG 1 cut(s) 281
BsaAI YACGTR 2 cut(s) 947, 1055
BsaBI GATNNNNATC 3 cut(s) 1087, 1117, 1123
BsaI GGTCTC 1 cut(s) 228
BsaJI CCNNGG 3 cut(s) 14, 872, 1064
BsaWI WCCGGW 1 cut(s) 1163
BsaXI ACNNNNNCTCC 2 cut(s) 61, 91
Bsc4I CCNNNNNNNGG 8 cut(s) 105, 163, 164, 188, 215, 597, 674, 1098
Bse118I RCCGGY 1 cut(s) 219
Bse1I ACTGG 7 cut(s) 158, 209, 380, 443, 506, 635, 1034
Bse3DI GCAATG 1 cut(s) 326
Bse8I GATNNNNATC 3 cut(s) 1087, 1117, 1123
BseBI CCWGG 1 cut(s) 16
BseDI CCNNGG 3 cut(s) 14, 872, 1064
BseGI GGATG 2 cut(s) 1011, 1088
BseJI GATNNNNATC 3 cut(s) 1087, 1117, 1123
BseLI CCNNNNNNNGG 8 cut(s) 105, 163, 164, 188, 215, 597, 674, 1098
BseMI GCAATG 1 cut(s) 326
BseMII CTCAG 1 cut(s) 116
BseNI ACTGG 7 cut(s) 158, 209, 380, 443, 506, 635, 1034
BseRI GAGGAG 5 cut(s) 34, 84, 121, 694, 1053
BseSI GKGCMC 1 cut(s) 592
BseX3I CGGCCG 1 cut(s) 964
BseXI GCAGC 1 cut(s) 624
Bsh1236I CGCG 2 cut(s) 643, 963
Bsh1285I CGRYCG 1 cut(s) 967
BshFI GGCC 3 cut(s) 219, 590, 966
BsiEI CGRYCG 1 cut(s) 967
BsiHKCI CYCGRG 1 cut(s) 1065
BsiSI CCGG 3 cut(s) 220, 281, 1164
BsiWI CGTACG 1 cut(s) 1224
BslI CCNNNNNNNGG 8 cut(s) 105, 163, 164, 188, 215, 597, 674, 1098
BsmAI GTCTC 4 cut(s) 228, 430, 795, 1063
BsnI GGCC 3 cut(s) 219, 590, 966
Bso31I GGTCTC 1 cut(s) 228
BsoBI CYCGRG 1 cut(s) 1065
Bsp120I GGGCCC 1 cut(s) 588
Bsp1286I GDGCHC 4 cut(s) 38, 263, 592, 1106
BspACI CCGC 6 cut(s) 574, 643, 736, 751, 963, 1021
BspANI GGCC 3 cut(s) 219, 590, 966
BspCNI CTCAG 1 cut(s) 115
BspFNI CGCG 2 cut(s) 643, 963
BspLI GGNNCC 6 cut(s) 260, 552, 590, 591, 983, 1103
BspMI ACCTGC 1 cut(s) 1164
BspOI GCTAGC 1 cut(s) 573
BspTNI GGTCTC 1 cut(s) 228
BsrDI GCAATG 1 cut(s) 326
BsrFI RCCGGY 1 cut(s) 219
BsrI ACTGG 7 cut(s) 158, 209, 380, 443, 506, 635, 1034
BssAI RCCGGY 1 cut(s) 219
BssECI CCNNGG 3 cut(s) 14, 872, 1064
BssT1I CCWWGG 1 cut(s) 872
Bst2UI CCWGG 1 cut(s) 16
Bst4CI ACNGT 1 cut(s) 608
Bst6I CTCTTC 1 cut(s) 724
BstBAI YACGTR 2 cut(s) 947, 1055
BstC8I GCNNGC 4 cut(s) 406, 469, 532, 571
BstDEI CTNAG 1 cut(s) 102
BstF5I GGATG 2 cut(s) 1011, 1088
BstFNI CGCG 2 cut(s) 643, 963
BstH2I RGCGCY 1 cut(s) 880
BstHHI GCGC 4 cut(s) 333, 643, 723, 879
BstMAI GTCTC 4 cut(s) 228, 430, 795, 1063
BstMCI CGRYCG 1 cut(s) 967
BstMWI GCNNNNNNNGC 3 cut(s) 153, 885, 927
BstNI CCWGG 1 cut(s) 16
BstSCI CCNGG 2 cut(s) 14, 279
BstSFI CTRYAG 2 cut(s) 880, 973
BstSLI GKGCMC 1 cut(s) 592
BstUI CGCG 2 cut(s) 643, 963
BstV1I GCAGC 1 cut(s) 624
BstX2I RGATCY 4 cut(s) 449, 512, 800, 1082
BstYI RGATCY 4 cut(s) 449, 512, 800, 1082
BstZI CGGCCG 1 cut(s) 964
BsuRI GGCC 3 cut(s) 219, 590, 966
BtsCI GGATG 2 cut(s) 1011, 1088
BtsIMutI CAGTG 2 cut(s) 216, 1041
BveI ACCTGC 1 cut(s) 1164
Cac8I GCNNGC 4 cut(s) 406, 469, 532, 571
CfoI GCGC 4 cut(s) 333, 643, 723, 879
Cfr10I RCCGGY 1 cut(s) 219
Cfr13I GGNCC 4 cut(s) 588, 589, 1131, 1161
CpoI CGGWCCG 1 cut(s) 1161
Csp6I GTAC 4 cut(s) 754, 772, 865, 1225
CspI CGGWCCG 1 cut(s) 1161
CviQI GTAC 4 cut(s) 754, 772, 865, 1225
DdeI CTNAG 1 cut(s) 102
EaeI YGGCCR 1 cut(s) 964
EagI CGGCCG 1 cut(s) 964
Eam1104I CTCTTC 1 cut(s) 724
EarI CTCTTC 1 cut(s) 724
EclXI CGGCCG 1 cut(s) 964
Eco130I CCWWGG 1 cut(s) 872
Eco24I GRGCYC 4 cut(s) 38, 263, 592, 1106
Eco31I GGTCTC 1 cut(s) 228
Eco47I GGWCC 2 cut(s) 1131, 1161
Eco52I CGGCCG 1 cut(s) 964
Eco72I CACGTG 1 cut(s) 1055
Eco88I CYCGRG 1 cut(s) 1065
EcoO109I RGGNCCY 3 cut(s) 588, 589, 1131
EcoRII CCWGG 1 cut(s) 14
EcoT14I CCWWGG 1 cut(s) 872
EcoT38I GRGCYC 4 cut(s) 38, 263, 592, 1106
ErhI CCWWGG 1 cut(s) 872
FalI AAGNNNNNCTT 2 cut(s) 682, 714
FauI CCCGC 1 cut(s) 729
Fnu4HI GCNGC 4 cut(s) 574, 613, 964, 1021
FokI GGATG 2 cut(s) 998, 1075
FriOI GRGCYC 4 cut(s) 38, 263, 592, 1106
Fsp4HI GCNGC 4 cut(s) 574, 613, 964, 1021
FspBI CTAG 7 cut(s) 558, 570, 630, 909, 1047, 1179, 1243
FspI TGCGCA 1 cut(s) 332
GlaI GCGC 4 cut(s) 332, 642, 722, 878
GluI GCNGC 4 cut(s) 574, 613, 964, 1021
GsuI CTGGAG 1 cut(s) 1017
HaeII RGCGCY 1 cut(s) 880
HaeIII GGCC 3 cut(s) 219, 590, 966
HapII CCGG 3 cut(s) 220, 281, 1164
HhaI GCGC 4 cut(s) 333, 643, 723, 879
Hin6I GCGC 4 cut(s) 331, 641, 721, 877
HinP1I GCGC 4 cut(s) 331, 641, 721, 877
HinfI GANTC 7 cut(s) 22, 127, 205, 244, 423, 625, 1142
HpaII CCGG 3 cut(s) 220, 281, 1164
HphI GGTGA 3 cut(s) 657, 693, 705
Hpy166II GTNNAC 1 cut(s) 314
Hpy188I TCNGA 4 cut(s) 27, 105, 998, 1161
Hpy8I GTNNAC 1 cut(s) 314
Hpy99I CGWCG 1 cut(s) 612
HpyAV CCTTC 8 cut(s) 190, 390, 453, 516, 564, 792, 849, 1204
HpyCH4III ACNGT 1 cut(s) 608
HpyCH4IV ACGT 2 cut(s) 946, 1054
HpyCH4V TGCA 3 cut(s) 467, 530, 764
HpyF10VI GCNNNNNNNGC 3 cut(s) 153, 885, 927
HpyF3I CTNAG 1 cut(s) 102
HpySE526I ACGT 2 cut(s) 946, 1054
HspAI GCGC 4 cut(s) 331, 641, 721, 877
LmnI GCTCC 7 cut(s) 108, 114, 258, 359, 681, 981, 1101
Lsp1109I GCAGC 1 cut(s) 624
LweI GCATC 1 cut(s) 126
MaeI CTAG 7 cut(s) 558, 570, 630, 909, 1047, 1179, 1243
MaeII ACGT 2 cut(s) 946, 1054
MaeIII GTNAC 2 cut(s) 47, 833
MboII GAAGA 5 cut(s) 507, 741, 909, 936, 1219
MflI RGATCY 4 cut(s) 449, 512, 800, 1082
MhlI GDGCHC 4 cut(s) 38, 263, 592, 1106
MluCI AATT 2 cut(s) 339, 1155
MlyI GAGTC 6 cut(s) 31, 136, 214, 253, 432, 634
MmeI TCCRAC 2 cut(s) 50, 337
MroXI GAANNNNTTC 2 cut(s) 697, 781
MseI TTAA 1 cut(s) 44
MspA1I CMGCKG 1 cut(s) 751
MspI CCGG 3 cut(s) 220, 281, 1164
MspR9I CCNGG 2 cut(s) 16, 281
MvaI CCWGG 1 cut(s) 16
MvnI CGCG 2 cut(s) 643, 963
MwoI GCNNNNNNNGC 3 cut(s) 153, 885, 927
NciI CCSGG 1 cut(s) 281
NheI GCTAGC 1 cut(s) 569
NlaIV GGNNCC 6 cut(s) 260, 552, 590, 591, 983, 1103
NmuCI GTSAC 2 cut(s) 47, 833
NsbI TGCGCA 1 cut(s) 332
PcsI WCGNNNNNNNCGW 2 cut(s) 829, 952
PdmI GAANNNNTTC 2 cut(s) 697, 781
PfeI GAWTC 1 cut(s) 1142
Pfl23II CGTACG 1 cut(s) 1224
PkrI GCNGC 4 cut(s) 575, 614, 965, 1022
PleI GAGTC 6 cut(s) 30, 135, 213, 252, 431, 633
PmaCI CACGTG 1 cut(s) 1055
PmlI CACGTG 1 cut(s) 1055
PpsI GAGTC 6 cut(s) 30, 135, 213, 252, 431, 633
Ppu21I YACGTR 2 cut(s) 947, 1055
PpuMI RGGWCCY 1 cut(s) 1131
Psp5II RGGWCCY 1 cut(s) 1131
Psp6I CCWGG 1 cut(s) 14
PspCI CACGTG 1 cut(s) 1055
PspGI CCWGG 1 cut(s) 14
PspLI CGTACG 1 cut(s) 1224
PspN4I GGNNCC 6 cut(s) 260, 552, 590, 591, 983, 1103
PspOMI GGGCCC 1 cut(s) 588
PspPI GGNCC 4 cut(s) 588, 589, 1131, 1161
PspPPI RGGWCCY 1 cut(s) 1131
PsuI RGATCY 4 cut(s) 449, 512, 800, 1082
RsaI GTAC 4 cut(s) 755, 773, 866, 1226
RsaNI GTAC 4 cut(s) 754, 772, 865, 1225
Rsr2I CGGWCCG 1 cut(s) 1161
RsrII CGGWCCG 1 cut(s) 1161
SaqAI TTAA 1 cut(s) 44
SatI GCNGC 4 cut(s) 574, 613, 964, 1021
Sau96I GGNCC 4 cut(s) 588, 589, 1131, 1161
SchI GAGTC 6 cut(s) 31, 136, 214, 253, 432, 634
ScrFI CCNGG 2 cut(s) 16, 281
SduI GDGCHC 4 cut(s) 38, 263, 592, 1106
SfaNI GCATC 1 cut(s) 126
SfcI CTRYAG 2 cut(s) 880, 973
SinI GGWCC 2 cut(s) 1131, 1161
SmlI CTYRAG 1 cut(s) 427
SmoI CTYRAG 1 cut(s) 427
Sse9I AATT 2 cut(s) 339, 1155
SsiI CCGC 6 cut(s) 574, 643, 736, 751, 963, 1021
SspMI CTAG 7 cut(s) 558, 570, 630, 909, 1047, 1179, 1243
StyD4I CCNGG 2 cut(s) 14, 279
StyI CCWWGG 1 cut(s) 872
TaaI ACNGT 1 cut(s) 608
TaiI ACGT 2 cut(s) 949, 1057
TaqI TCGA 6 cut(s) 74, 394, 457, 520, 823, 860
TasI AATT 2 cut(s) 339, 1155
TauI GCSGC 3 cut(s) 576, 966, 1023
TfiI GAWTC 1 cut(s) 1142
Tru1I TTAA 1 cut(s) 44
Tru9I TTAA 1 cut(s) 44
TscAI CASTG 2 cut(s) 216, 1041
TseFI GTSAC 2 cut(s) 47, 833
TseI GCWGC 1 cut(s) 612
Tsp45I GTSAC 2 cut(s) 47, 833
TspGWI ACGGA 2 cut(s) 70, 781
TspRI CASTG 2 cut(s) 216, 1041
VpaK11BI GGWCC 2 cut(s) 1131, 1161
XbaI TCTAGA 1 cut(s) 1046
XcmI CCANNNNNNNNNTGG 1 cut(s) 189
XmnI GAANNNNTTC 2 cut(s) 697, 781
XspI CTAG 7 cut(s) 558, 570, 630, 909, 1047, 1179, 1243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.