MD15G1137100.v1.1

Aminotransferase class-V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010521
Physical Location & Seq
Reverse (-)
10044558 .. 10046946
2389 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1137100.v1.1.491

Sequence Viewer

Length: 1113 bp
ATGATTTTTTATCAACTAGTTTGTGGGCCGTATGTGGAAATTGAGATGAGGAGTGGACAGATAGACGGGTACGATGGTATTTTCCTGAGCCCACACAAGTTTGTAGGTGGGCCCGGAACCCCAGGCATACTTGTGATGAGCAAGGCACTGTATCGGCTCAAGTCTTTTCCCCCATCAACTTGTGGAGGTGGGACTGTTGCTTACGTCAATGGCTTCAATCCTGAGGACACAGTGTACTGCGAGGACATAGAAGAGAGGGAAAACGGCGGTACTCCTCAAATCATCCAAACAATTAGAGCAGCCTTGGCTTTCTGGGTGAAGGAATACATTGGTTATCAAATCATTGAAAAGCAAGAGCAACTCCATGTTAAAAGGGCACTAGAGAGGCTTCTCCCTAACCAAAACATATGGATTTTAGGAAATACGACCGCCAAAAGACTAGCCATTTTGTCCTTCCTCGTTTATTCTACGACCAATTGCTCATCAACGGCTGCCACGAAAACAGATATTGGCAGAGAGGGAGCTGACCAAGGGCTCTACATGTGGGGAGAGACGGGAAACAGCAGAGGCAAGCCACTTCATGGGGGTTTCGTCGCAGCTCTGCTCAATGATCTATTCGGTATTCAGGCTCGAGGCGGCTGCGCTTGTGCTGGACCCTATGGTCACAAGCTCCTCAAAATTGATCACACTGTTTCACATGCCTATAGATCTTCCATTCAGAAGGACTATCTTGGAGTGAAACCAGGGTGGACTAGAATCAGCTTTCCCTACTACATGTCAAACGATGAACTGGAGTTCATTTTAGCTGCGCTGGAGTTCCTAGCTACATATGGACAACGTTTTCTTCCCCTATATAACTTCAGTTTGATAAGTGGAAGTTGGACTTGCGATCAGAAGGCACTTCAAGACCTAATTGGGAAGACCAACTTCAACTTCGATGTCGTTAGTTTGCCCACCCATGGGGACAATGCTACACAGGGAATGTCATCTAAAGACAGAAACAATAAGCTGCTCGCTACCGCTACAAGCATTGCGAATTGCTTGCCCAAGTTTCCTCCTCAGCGTAGGCTTCTGGAAGACATAGACATCAACATGTTGTACTTCAGAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_5

Protein Analysis

371

Amino Acids

41.1

Weight (kDa)

7.56

Isoelectric Point (pI)

38.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_5 PF00266 23 - 154 1.3e-10 Aminotransferase class-V
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 660
AccB7I CCANNNNNTGG 1 cut(s) 581
AciI CCGC 4 cut(s) 267, 429, 636, 1020
AclI AACGTT 1 cut(s) 838
AcuI CTGAAG 2 cut(s) 844, 1087
AfaI GTAC 4 cut(s) 71, 236, 271, 1100
AfiI CCNNNNNNNGG 3 cut(s) 581, 959, 960
AflIII ACRYGT 3 cut(s) 540, 774, 1092
AgsI TTSAA 4 cut(s) 217, 347, 905, 931
AhlI ACTAGT 1 cut(s) 16
AjnI CCWGG 2 cut(s) 121, 742
AjuI GAANNNNNNNTTGG 2 cut(s) 917, 949
AluBI AGCT 7 cut(s) 524, 599, 670, 762, 806, 824, 1009
AluI AGCT 7 cut(s) 524, 599, 670, 762, 806, 824, 1009
Alw26I GTCTC 1 cut(s) 545
Ama87I CYCGRG 1 cut(s) 630
AoxI GGCC 2 cut(s) 26, 110
ApaI GGGCCC 1 cut(s) 114
ApeKI GCWGC 6 cut(s) 299, 491, 596, 639, 806, 1009
AspLEI GCGC 2 cut(s) 644, 811
AspS9I GGNCC 4 cut(s) 26, 110, 111, 653
AsuC2I CCSGG 1 cut(s) 114
AsuHPI GGTGA 1 cut(s) 328
AvaI CYCGRG 1 cut(s) 630
AvaII GGWCC 1 cut(s) 653
AxyI CCTNAGG 1 cut(s) 222
BaeGI GKGCMC 2 cut(s) 114, 379
BaeI ACNNNNGTAYC 2 cut(s) 61, 94
BanII GRGCYC 3 cut(s) 92, 114, 537
BbsI GAAGAC 2 cut(s) 926, 1083
BbvCI CCTCAGC 1 cut(s) 1059
BbvI GCAGC 6 cut(s) 311, 478, 608, 626, 793, 996
BccI CCATC 2 cut(s) 68, 181
BceAI ACGGC 3 cut(s) 13, 280, 504
BcgI CGANNNNNNTGC 4 cut(s) 621, 655, 1024, 1058
BciT130I CCWGG 2 cut(s) 123, 744
BclI TGATCA 1 cut(s) 682
BcnI CCSGG 1 cut(s) 114
BcoDI GTCTC 1 cut(s) 545
BcuI ACTAGT 1 cut(s) 16
BfaI CTAG 5 cut(s) 17, 380, 440, 753, 821
BfmI CTRYAG 1 cut(s) 703
BglII AGATCT 1 cut(s) 707
BisI GCNGC 7 cut(s) 300, 492, 597, 637, 640, 807, 1010
BlsI GCNGC 7 cut(s) 301, 493, 598, 638, 641, 808, 1011
Bme1390I CCNGG 3 cut(s) 114, 123, 744
Bme18I GGWCC 1 cut(s) 653
BmeT110I CYCGRG 1 cut(s) 630
BmgT120I GGNCC 4 cut(s) 26, 110, 111, 653
BmiI GGNNCC 3 cut(s) 112, 118, 655
BmrFI CCNGG 3 cut(s) 114, 123, 744
BpiI GAAGAC 2 cut(s) 926, 1083
BpmI CTGGAG 2 cut(s) 812, 833
Bpu10I CCTNAGC 2 cut(s) 86, 1059
BpuEI CTTGAG 1 cut(s) 143
BpuMI CCSGG 1 cut(s) 114
BsaJI CCNNGG 5 cut(s) 121, 303, 529, 743, 958
Bsc4I CCNNNNNNNGG 3 cut(s) 581, 959, 960
Bse1I ACTGG 1 cut(s) 795
Bse21I CCTNAGG 1 cut(s) 222
Bse3DI GCAATG 1 cut(s) 1029
BseBI CCWGG 2 cut(s) 123, 744
BseDI CCNNGG 5 cut(s) 121, 303, 529, 743, 958
BseGI GGATG 1 cut(s) 282
BseLI CCNNNNNNNGG 3 cut(s) 581, 959, 960
BseMI GCAATG 1 cut(s) 1029
BseMII CTCAG 3 cut(s) 77, 213, 1073
BseNI ACTGG 1 cut(s) 795
BseRI GAGGAG 4 cut(s) 64, 264, 662, 1047
BseSI GKGCMC 2 cut(s) 114, 379
BseXI GCAGC 6 cut(s) 311, 478, 608, 626, 793, 996
Bsh1285I CGRYCG 1 cut(s) 429
BshFI GGCC 2 cut(s) 28, 112
BsiEI CGRYCG 1 cut(s) 429
BsiHKCI CYCGRG 1 cut(s) 630
BsiSI CCGG 1 cut(s) 114
BslFI GGGAC 2 cut(s) 205, 977
BslI CCNNNNNNNGG 3 cut(s) 581, 959, 960
BsmAI GTCTC 1 cut(s) 545
BsmBI CGTCTC 1 cut(s) 545
BsmFI GGGAC 2 cut(s) 205, 977
BsnI GGCC 2 cut(s) 28, 112
BsoBI CYCGRG 1 cut(s) 630
Bsp120I GGGCCC 1 cut(s) 110
Bsp1286I GDGCHC 4 cut(s) 92, 114, 379, 537
Bsp143I GATC 4 cut(s) 610, 682, 707, 889
Bsp19I CCATGG 1 cut(s) 958
BspACI CCGC 4 cut(s) 267, 429, 636, 1020
BspANI GGCC 2 cut(s) 28, 112
BspCNI CTCAG 3 cut(s) 78, 214, 1072
BspLI GGNNCC 3 cut(s) 112, 118, 655
BsrDI GCAATG 1 cut(s) 1029
BsrI ACTGG 1 cut(s) 795
BssECI CCNNGG 5 cut(s) 121, 303, 529, 743, 958
BssMI GATC 4 cut(s) 610, 682, 707, 889
BssT1I CCWWGG 3 cut(s) 303, 529, 958
Bst2UI CCWGG 2 cut(s) 123, 744
Bst4CI ACNGT 4 cut(s) 150, 196, 232, 691
Bst6I CTCTTC 1 cut(s) 246
BstC8I GCNNGC 3 cut(s) 572, 1014, 1043
BstDEI CTNAG 3 cut(s) 86, 222, 1059
BstDSI CCRYGG 1 cut(s) 958
BstF5I GGATG 1 cut(s) 282
BstHHI GCGC 2 cut(s) 644, 811
BstKTI GATC 4 cut(s) 613, 685, 710, 892
BstMAI GTCTC 1 cut(s) 545
BstMBI GATC 4 cut(s) 610, 682, 707, 889
BstMCI CGRYCG 1 cut(s) 429
BstMWI GCNNNNNNNGC 1 cut(s) 305
BstNI CCWGG 2 cut(s) 123, 744
BstNSI RCATGY 4 cut(s) 544, 701, 778, 1096
BstSCI CCNGG 3 cut(s) 112, 121, 742
BstSFI CTRYAG 1 cut(s) 703
BstSLI GKGCMC 2 cut(s) 114, 379
BstV1I GCAGC 6 cut(s) 311, 478, 608, 626, 793, 996
BstV2I GAAGAC 2 cut(s) 926, 1083
BstX2I RGATCY 1 cut(s) 707
BstYI RGATCY 1 cut(s) 707
Bsu36I CCTNAGG 1 cut(s) 222
BsuRI GGCC 2 cut(s) 28, 112
BtgI CCRYGG 1 cut(s) 958
BtsCI GGATG 1 cut(s) 282
BtsIMutI CAGTG 3 cut(s) 146, 237, 687
Cac8I GCNNGC 3 cut(s) 572, 1014, 1043
CfoI GCGC 2 cut(s) 644, 811
Cfr13I GGNCC 4 cut(s) 26, 110, 111, 653
Csp6I GTAC 4 cut(s) 70, 235, 270, 1099
CviAII CATG 7 cut(s) 365, 541, 581, 698, 775, 959, 1093
CviQI GTAC 4 cut(s) 70, 235, 270, 1099
DdeI CTNAG 3 cut(s) 86, 222, 1059
DpnI GATC 4 cut(s) 612, 684, 709, 891
DpnII GATC 4 cut(s) 610, 682, 707, 889
DrdI GACNNNNNNGTC 1 cut(s) 660
DseDI GACNNNNNNGTC 1 cut(s) 660
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
Eco130I CCWWGG 3 cut(s) 303, 529, 958
Eco24I GRGCYC 3 cut(s) 92, 114, 537
Eco47I GGWCC 1 cut(s) 653
Eco57I CTGAAG 2 cut(s) 844, 1087
Eco81I CCTNAGG 1 cut(s) 222
Eco88I CYCGRG 1 cut(s) 630
EcoRII CCWGG 2 cut(s) 121, 742
EcoT14I CCWWGG 3 cut(s) 303, 529, 958
EcoT38I GRGCYC 3 cut(s) 92, 114, 537
ErhI CCWWGG 3 cut(s) 303, 529, 958
Esp3I CGTCTC 1 cut(s) 545
FaeI CATG 7 cut(s) 368, 544, 584, 701, 778, 962, 1096
FalI AAGNNNNNCTT 4 cut(s) 868, 900, 911, 943
FaqI GGGAC 2 cut(s) 205, 977
FatI CATG 7 cut(s) 364, 540, 580, 697, 774, 958, 1092
FauNDI CATATG 2 cut(s) 407, 829
FbaI TGATCA 1 cut(s) 682
Fnu4HI GCNGC 7 cut(s) 300, 492, 597, 637, 640, 807, 1010
FokI GGATG 1 cut(s) 269
FriOI GRGCYC 3 cut(s) 92, 114, 537
Fsp4HI GCNGC 7 cut(s) 300, 492, 597, 637, 640, 807, 1010
FspBI CTAG 5 cut(s) 17, 380, 440, 753, 821
GlaI GCGC 2 cut(s) 643, 810
GluI GCNGC 7 cut(s) 300, 492, 597, 637, 640, 807, 1010
GsuI CTGGAG 2 cut(s) 812, 833
HaeIII GGCC 2 cut(s) 28, 112
HapII CCGG 1 cut(s) 114
HhaI GCGC 2 cut(s) 644, 811
Hin1II CATG 7 cut(s) 368, 544, 584, 701, 778, 962, 1096
Hin6I GCGC 2 cut(s) 642, 809
HinP1I GCGC 2 cut(s) 642, 809
HinfI GANTC 2 cut(s) 756, 1107
HpaII CCGG 1 cut(s) 114
HphI GGTGA 1 cut(s) 328
Hpy166II GTNNAC 3 cut(s) 56, 235, 750
Hpy188I TCNGA 3 cut(s) 720, 894, 1106
Hpy188III TCNNGA 4 cut(s) 85, 221, 905, 1073
Hpy8I GTNNAC 3 cut(s) 56, 235, 750
Hpy99I CGWCG 1 cut(s) 596
HpyAV CCTTC 4 cut(s) 313, 463, 715, 889
HpyCH4III ACNGT 4 cut(s) 150, 196, 232, 691
HpyCH4IV ACGT 2 cut(s) 204, 838
HpyF10VI GCNNNNNNNGC 1 cut(s) 305
HpyF3I CTNAG 3 cut(s) 86, 222, 1059
HpySE526I ACGT 2 cut(s) 204, 838
Hsp92II CATG 7 cut(s) 368, 544, 584, 701, 778, 962, 1096
HspAI GCGC 2 cut(s) 642, 809
Ksp22I TGATCA 1 cut(s) 682
Kzo9I GATC 4 cut(s) 610, 682, 707, 889
LmnI GCTCC 2 cut(s) 521, 675
Lsp1109I GCAGC 6 cut(s) 311, 478, 608, 626, 793, 996
MaeI CTAG 5 cut(s) 17, 380, 440, 753, 821
MaeII ACGT 2 cut(s) 204, 838
MaeIII GTNAC 1 cut(s) 662
MalI GATC 4 cut(s) 612, 684, 709, 891
MboI GATC 4 cut(s) 610, 682, 707, 889
MboII GAAGA 5 cut(s) 263, 702, 836, 931, 1088
MfeI CAATTG 1 cut(s) 475
MflI RGATCY 1 cut(s) 707
MhlI GDGCHC 4 cut(s) 92, 114, 379, 537
MluCI AATT 6 cut(s) 39, 291, 475, 678, 912, 1036
MmeI TCCRAC 1 cut(s) 860
MseI TTAA 1 cut(s) 369
MslI CAYNNNNRTG 2 cut(s) 131, 1091
MspI CCGG 1 cut(s) 114
MspR9I CCNGG 3 cut(s) 114, 123, 744
MunI CAATTG 1 cut(s) 475
MvaI CCWGG 2 cut(s) 123, 744
MwoI GCNNNNNNNGC 1 cut(s) 305
NciI CCSGG 1 cut(s) 114
NcoI CCATGG 1 cut(s) 958
NdeI CATATG 2 cut(s) 407, 829
NdeII GATC 4 cut(s) 610, 682, 707, 889
NlaIII CATG 7 cut(s) 368, 544, 584, 701, 778, 962, 1096
NlaIV GGNNCC 3 cut(s) 112, 118, 655
NmuCI GTSAC 1 cut(s) 662
NspI RCATGY 4 cut(s) 544, 701, 778, 1096
PaeR7I CTCGAG 1 cut(s) 630
PciI ACATGT 3 cut(s) 540, 774, 1092
PcsI WCGNNNNNNNCGW 1 cut(s) 494
PfeI GAWTC 1 cut(s) 756
PflMI CCANNNNNTGG 1 cut(s) 581
PkrI GCNGC 7 cut(s) 301, 493, 598, 638, 641, 808, 1011
PscI ACATGT 3 cut(s) 540, 774, 1092
Psp1406I AACGTT 1 cut(s) 838
Psp6I CCWGG 2 cut(s) 121, 742
PspGI CCWGG 2 cut(s) 121, 742
PspN4I GGNNCC 3 cut(s) 112, 118, 655
PspOMI GGGCCC 1 cut(s) 110
PspPI GGNCC 4 cut(s) 26, 110, 111, 653
PspXI VCTCGAGB 1 cut(s) 630
PsuI RGATCY 1 cut(s) 707
RsaI GTAC 4 cut(s) 71, 236, 271, 1100
RsaNI GTAC 4 cut(s) 70, 235, 270, 1099
RseI CAYNNNNRTG 2 cut(s) 131, 1091
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 7 cut(s) 300, 492, 597, 637, 640, 807, 1010
Sau3AI GATC 4 cut(s) 610, 682, 707, 889
Sau96I GGNCC 4 cut(s) 26, 110, 111, 653
ScrFI CCNGG 3 cut(s) 114, 123, 744
SduI GDGCHC 4 cut(s) 92, 114, 379, 537
SfcI CTRYAG 1 cut(s) 703
Sfr274I CTCGAG 1 cut(s) 630
SinI GGWCC 1 cut(s) 653
SlaI CTCGAG 1 cut(s) 630
SmiMI CAYNNNNRTG 2 cut(s) 131, 1091
SmlI CTYRAG 2 cut(s) 158, 630
SmoI CTYRAG 2 cut(s) 158, 630
SpeI ACTAGT 1 cut(s) 16
Sse9I AATT 6 cut(s) 39, 291, 475, 678, 912, 1036
SsiI CCGC 4 cut(s) 267, 429, 636, 1020
SspMI CTAG 5 cut(s) 17, 380, 440, 753, 821
StyD4I CCNGG 3 cut(s) 112, 121, 742
StyI CCWWGG 3 cut(s) 303, 529, 958
TaaI ACNGT 4 cut(s) 150, 196, 232, 691
TaiI ACGT 2 cut(s) 207, 841
TaqI TCGA 2 cut(s) 631, 936
TasI AATT 6 cut(s) 39, 291, 475, 678, 912, 1036
TatI WGTACW 2 cut(s) 234, 1098
TauI GCSGC 1 cut(s) 639
TfiI GAWTC 1 cut(s) 756
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 3 cut(s) 153, 237, 694
TseFI GTSAC 1 cut(s) 662
TseI GCWGC 6 cut(s) 299, 491, 596, 639, 806, 1009
Tsp45I GTSAC 1 cut(s) 662
TspDTI ATGAA 3 cut(s) 569, 787, 801
TspRI CASTG 3 cut(s) 153, 237, 694
Van91I CCANNNNNTGG 1 cut(s) 581
VpaK11BI GGWCC 1 cut(s) 653
XceI RCATGY 4 cut(s) 544, 701, 778, 1096
XhoI CTCGAG 1 cut(s) 630
XspI CTAG 5 cut(s) 17, 380, 440, 753, 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.