MD15G1149700.v1.1

Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0028408
Physical Location & Seq
Forward (+)
11053940 .. 11054290
351 bp
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UTR
Exon/CDS
Intron
MD15G1149700.v1.1.491

Sequence Viewer

Length: 351 bp
ATGGCTACCCCCTCCGGGCACCTCCTCCTTAAGCTGCTTGTTTGCACGGTGCTGGTGCTTATGGTGGCGGCTCTTGCGCCCAAGGCTGAAGAGGGAACCCTCGTTTGTGATATTATTCAGGTGCAGGAAACCATGTGGGCATGCAGACCATACATCTTTAAACGTGGGGAGTTGACTAAAGCTTGCTGCAATGGGGTCAAGACCGTCTACAGAAATGTTAAGACCGCCGTTGACCGCCAGGCGGCCTGCGAGTGCATCATAGATTTAGCCCCGTTTAATCGTACTAGTGAAACCCGGATTGCTGGCCTTCCTACCAAGTGTGGGATTCCCAACAATAACTGCACAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0006810 GO:0006869 GO:0008150 GO:0010876 GO:0016020 GO:0033036 GO:0051179 GO:0051234 GO:0071702
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2 Tryp_alpha_amyl

Protein Analysis

117

Amino Acids

12.63

Weight (kDa)

8.62

Isoelectric Point (pI)

41.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 42 - 114 7.2e-07 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028408)

Species Orthologous Gene IDs
malus_domestica MD15G1149700.v1.1
pyrus_communis pycom15g13380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 18
AccI GTMKAC 1 cut(s) 207
AciI CCGC 4 cut(s) 68, 225, 235, 242
AcuI CTGAAG 1 cut(s) 108
AfaI GTAC 1 cut(s) 283
AfiI CCNNNNNNNGG 3 cut(s) 15, 241, 321
AflII CTTAAG 1 cut(s) 29
AhlI ACTAGT 1 cut(s) 284
AjnI CCWGG 1 cut(s) 237
AluBI AGCT 2 cut(s) 34, 182
AluI AGCT 2 cut(s) 34, 182
AoxI GGCC 2 cut(s) 243, 304
ApeKI GCWGC 2 cut(s) 34, 186
AspLEI GCGC 1 cut(s) 79
AsuC2I CCSGG 2 cut(s) 16, 295
BaeGI GKGCMC 1 cut(s) 21
BanI GGYRCC 1 cut(s) 18
BbvI GCAGC 2 cut(s) 21, 173
BceAI ACGGC 1 cut(s) 212
BciT130I CCWGG 1 cut(s) 239
BcnI CCSGG 2 cut(s) 16, 295
BcuI ACTAGT 1 cut(s) 284
BfaI CTAG 1 cut(s) 285
BfmI CTRYAG 1 cut(s) 208
BfrI CTTAAG 1 cut(s) 29
BisI GCNGC 4 cut(s) 35, 69, 187, 243
BlsI GCNGC 4 cut(s) 36, 70, 188, 244
Bme1390I CCNGG 3 cut(s) 16, 239, 295
BmiI GGNNCC 2 cut(s) 20, 97
BmrFI CCNGG 3 cut(s) 16, 239, 295
BmsI GCATC 1 cut(s) 264
BpuMI CCSGG 2 cut(s) 16, 295
BsaJI CCNNGG 1 cut(s) 81
Bsc4I CCNNNNNNNGG 3 cut(s) 15, 241, 321
Bse3DI GCAATG 1 cut(s) 196
BseBI CCWGG 1 cut(s) 239
BseDI CCNNGG 1 cut(s) 81
BseLI CCNNNNNNNGG 3 cut(s) 15, 241, 321
BseMI GCAATG 1 cut(s) 196
BseRI GAGGAG 1 cut(s) 14
BseSI GKGCMC 1 cut(s) 21
BseXI GCAGC 2 cut(s) 21, 173
BsgI GTGCAG 2 cut(s) 143, 325
BshFI GGCC 2 cut(s) 245, 306
BshNI GGYRCC 1 cut(s) 18
BsiSI CCGG 2 cut(s) 15, 295
BslI CCNNNNNNNGG 3 cut(s) 15, 241, 321
BsnI GGCC 2 cut(s) 245, 306
Bsp1286I GDGCHC 1 cut(s) 21
BspACI CCGC 4 cut(s) 68, 225, 235, 242
BspANI GGCC 2 cut(s) 245, 306
BspLI GGNNCC 2 cut(s) 20, 97
BspT107I GGYRCC 1 cut(s) 18
BspTI CTTAAG 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 196
BssECI CCNNGG 1 cut(s) 81
BssT1I CCWWGG 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 239
Bst4CI ACNGT 2 cut(s) 49, 205
Bst6I CTCTTC 1 cut(s) 84
BstAFI CTTAAG 1 cut(s) 29
BstC8I GCNNGC 4 cut(s) 142, 184, 247, 304
BstHHI GCGC 1 cut(s) 79
BstMWI GCNNNNNNNGC 2 cut(s) 74, 83
BstNI CCWGG 1 cut(s) 239
BstNSI RCATGY 1 cut(s) 144
BstSCI CCNGG 3 cut(s) 14, 237, 293
BstSFI CTRYAG 1 cut(s) 208
BstSLI GKGCMC 1 cut(s) 21
BstV1I GCAGC 2 cut(s) 21, 173
BsuRI GGCC 2 cut(s) 245, 306
Cac8I GCNNGC 4 cut(s) 142, 184, 247, 304
CfoI GCGC 1 cut(s) 79
Csp6I GTAC 1 cut(s) 282
CviAII CATG 2 cut(s) 133, 141
CviJI RGCY 8 cut(s) 5, 34, 71, 86, 182, 245, 269, 306
CviKI_1 RGCY 8 cut(s) 5, 34, 71, 86, 182, 245, 269, 306
CviQI GTAC 1 cut(s) 282
DraI TTTAAA 1 cut(s) 160
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
Eco130I CCWWGG 1 cut(s) 81
Eco57I CTGAAG 1 cut(s) 108
EcoRII CCWGG 1 cut(s) 237
EcoT14I CCWWGG 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 81
FaeI CATG 2 cut(s) 136, 144
FaiI YATR 6 cut(s) 62, 134, 142, 151, 260, 349
FatI CATG 2 cut(s) 132, 140
FblI GTMKAC 1 cut(s) 207
Fnu4HI GCNGC 4 cut(s) 35, 69, 187, 243
Fsp4HI GCNGC 4 cut(s) 35, 69, 187, 243
FspBI CTAG 1 cut(s) 285
GlaI GCGC 1 cut(s) 78
GluI GCNGC 4 cut(s) 35, 69, 187, 243
HaeIII GGCC 2 cut(s) 245, 306
HapII CCGG 2 cut(s) 15, 295
HhaI GCGC 1 cut(s) 79
Hin1II CATG 2 cut(s) 136, 144
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HincII GTYRAC 2 cut(s) 174, 232
HindII GTYRAC 2 cut(s) 174, 232
HindIII AAGCTT 1 cut(s) 180
HinfI GANTC 1 cut(s) 325
HpaII CCGG 2 cut(s) 15, 295
Hpy166II GTNNAC 3 cut(s) 174, 208, 232
Hpy188III TCNNGA 1 cut(s) 199
Hpy8I GTNNAC 3 cut(s) 174, 208, 232
HpyAV CCTTC 1 cut(s) 317
HpyCH4III ACNGT 2 cut(s) 49, 205
HpyCH4IV ACGT 1 cut(s) 163
HpyCH4V TGCA 6 cut(s) 45, 124, 144, 189, 255, 342
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 83
HpySE526I ACGT 1 cut(s) 163
Hsp92II CATG 2 cut(s) 136, 144
HspAI GCGC 1 cut(s) 77
LpnPI CCDG 9 cut(s) 28, 38, 104, 110, 224, 251, 259, 288, 308
Lsp1109I GCAGC 2 cut(s) 21, 173
LweI GCATC 1 cut(s) 264
MaeI CTAG 1 cut(s) 285
MaeII ACGT 1 cut(s) 163
MboII GAAGA 1 cut(s) 101
MhlI GDGCHC 1 cut(s) 21
MnlI CCTC 5 cut(s) 22, 32, 35, 85, 110
MseI TTAA 4 cut(s) 30, 159, 219, 276
MspCI CTTAAG 1 cut(s) 29
MspI CCGG 2 cut(s) 15, 295
MspR9I CCNGG 3 cut(s) 16, 239, 295
MvaI CCWGG 1 cut(s) 239
MwoI GCNNNNNNNGC 2 cut(s) 74, 83
NciI CCSGG 2 cut(s) 16, 295
NlaIII CATG 2 cut(s) 136, 144
NlaIV GGNNCC 2 cut(s) 20, 97
NspI RCATGY 1 cut(s) 144
PaeI GCATGC 1 cut(s) 144
PfeI GAWTC 1 cut(s) 325
PkrI GCNGC 4 cut(s) 36, 70, 188, 244
Psp6I CCWGG 1 cut(s) 237
PspGI CCWGG 1 cut(s) 237
PspN4I GGNNCC 2 cut(s) 20, 97
RsaI GTAC 1 cut(s) 283
RsaNI GTAC 1 cut(s) 282
SaqAI TTAA 4 cut(s) 30, 159, 219, 276
SatI GCNGC 4 cut(s) 35, 69, 187, 243
ScrFI CCNGG 3 cut(s) 16, 239, 295
SduI GDGCHC 1 cut(s) 21
SetI ASST 5 cut(s) 24, 36, 123, 166, 184
SfaNI GCATC 1 cut(s) 264
SfcI CTRYAG 1 cut(s) 208
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
SpeI ACTAGT 1 cut(s) 284
SphI GCATGC 1 cut(s) 144
SsiI CCGC 4 cut(s) 68, 225, 235, 242
SspMI CTAG 1 cut(s) 285
StyD4I CCNGG 3 cut(s) 14, 237, 293
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 2 cut(s) 49, 205
TaiI ACGT 1 cut(s) 166
TauI GCSGC 2 cut(s) 71, 245
TfiI GAWTC 1 cut(s) 325
Tru1I TTAA 4 cut(s) 30, 159, 219, 276
Tru9I TTAA 4 cut(s) 30, 159, 219, 276
TseI GCWGC 2 cut(s) 34, 186
Vha464I CTTAAG 1 cut(s) 29
XceI RCATGY 1 cut(s) 144
XmiI GTMKAC 1 cut(s) 207
XspI CTAG 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.