MD15G1151800.v1.1

APO protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0026694
Physical Location & Seq
Forward (+)
11176036 .. 11178403
2368 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1151800.v1.1.491

Sequence Viewer

Length: 1023 bp
ATGTTCTCAGTAAAATGGAAAAAGGAGCAAGGGAGGGACTCCGTGGTGCAAGTGACAAAGGAAGCCTATGAGACCTGCGTCATTTCAAAGCCAATCAAAGCAGACGTGGAGGAGATCACGTTGAATAAGTCAGGACCATTCTGCTTCATCAGTGGTGTTAAGGAGCACTGTGACATGGGCTTAAAGCTCACGGTGGTGGTTCTGTCTGAAAAACATAAGTATGGTTCGGATTCTCCGTCGCCTGCTCCAGCTGCAGCTCCGTCGAGTAACGCTGCTGCTCGTGGTTTCAGTTTGAGTGCTGCGTTTATTGCGGTTGGGGTGGCAGTGGGGGGATGGCTTTGTTTTGAGCGTGTGAGACAACGGACTGATTATGAAAAACACGAACACAGAGCGAAAGCACATGGTCGTCGAAACGCTCTCTGCTGTCATCTTTGCTGCGAAAGCGAAAGCACTTTGCTTTCAGAAATAGATTGCAGCAGCTCTCATAGCTTCTCCCATGGCGCCGTAGCTTCATTCCACGGAAAAGGAACAACCTTTCTTCCTCCAAAAGTCGGCACTTTTCGGGCTTCCTTCAGTTCAAAATCAGAATTCCTGAATTCCCACCTGTACCCTTCTCTCACTTCCCTCGATTCTTTCCGATTTCAGCAGGGTTGCAATGTGCTGAAGATTTCAGTTTCAGTGAAGTCGAGAACTTCGAAATCATATGCATTGGTGGTGAGGAGTGACCACCGTCATCCCCAGAACTCCGACTTTCCGCAGCACTATTCGAAGAAGGAGAAGAAGTCCTTTCCGGTGCCAATTGTGGAGCTGAGACGGGCGGCGAGGGAGAGGCTTAAGAGCAACAAGGGAAAGCCTAGGACCCCAGTTCCACCTCTCAAAAATGGCTTGCTCGTGAAGACCCTTGTGCCGGTTGCGTATGATGTGTTTGATGAGAGAATTACATTGATCAACAATTTGAGGAGGCTTTTGAAGGTGGTTCCTGTGCAAGCTTGCAGGTTAGCTTGTGGAGCTTTTCTGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APO_RNA-bind

Protein Analysis

341

Amino Acids

37.61

Weight (kDa)

9.68

Isoelectric Point (pI)

46.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 8 - 60 2.1e-07 Plastocyanin-like domain
APO_RNA-bind PF05634 240 - 333 1.5e-31 APO RNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0026694)

Species Orthologous Gene IDs
malus_domestica MD15G1151800.v1.1
pyrus_communis pycom10g19370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 83, 984
AccB1I GGYRCC 2 cut(s) 500, 793
AciI CCGC 3 cut(s) 311, 755, 818
AcsI RAATTY 2 cut(s) 587, 595
AcuI CTGAAG 2 cut(s) 556, 683
AcyI GRCGYC 1 cut(s) 501
AfaI GTAC 1 cut(s) 608
AfiI CCNNNNNNNGG 2 cut(s) 551, 907
AflII CTTAAG 1 cut(s) 833
AgsI TTSAA 4 cut(s) 87, 124, 579, 970
AjiI CACGTC 1 cut(s) 106
AleI CACNNNNGTG 1 cut(s) 194
Alw21I GWGCWC 1 cut(s) 168
Alw26I GTCTC 3 cut(s) 65, 349, 805
ApeKI GCWGC 9 cut(s) 251, 254, 272, 275, 299, 435, 474, 477, 757
ApoI RAATTY 2 cut(s) 587, 595
AspA2I CCTAGG 1 cut(s) 854
AspLEI GCGC 1 cut(s) 503
AspS9I GGNCC 2 cut(s) 134, 858
AsuHPI GGTGA 1 cut(s) 727
AsuII TTCGAA 2 cut(s) 695, 767
AvaII GGWCC 2 cut(s) 134, 858
AvrII CCTAGG 1 cut(s) 854
BanI GGYRCC 2 cut(s) 500, 793
BauI CACGAG 2 cut(s) 279, 890
BbsI GAAGAC 1 cut(s) 902
Bbv12I GWGCWC 1 cut(s) 168
BbvI GCAGC 9 cut(s) 238, 259, 262, 266, 286, 422, 486, 489, 769
BccI CCATC 1 cut(s) 327
BceAI ACGGC 1 cut(s) 488
BcgI CGANNNNNNTGC 2 cut(s) 243, 277
BclI TGATCA 1 cut(s) 945
BcoDI GTCTC 3 cut(s) 65, 349, 805
BfaI CTAG 1 cut(s) 855
BfmI CTRYAG 1 cut(s) 252
BfoI RGCGCY 1 cut(s) 504
BfrI CTTAAG 1 cut(s) 833
BfuAI ACCTGC 2 cut(s) 83, 984
BlnI CCTAGG 1 cut(s) 854
Bme18I GGWCC 2 cut(s) 134, 858
BmgBI CACGTC 1 cut(s) 106
BmgT120I GGNCC 2 cut(s) 134, 858
BmiI GGNNCC 4 cut(s) 502, 795, 860, 978
BmrI ACTGGG 1 cut(s) 857
BmuI ACTGGG 1 cut(s) 857
BoxI GACNNNNGTC 2 cut(s) 77, 729
BpiI GAAGAC 1 cut(s) 902
BpmI CTGGAG 1 cut(s) 231
Bpu14I TTCGAA 2 cut(s) 695, 767
BsaHI GRCGYC 1 cut(s) 501
BsaI GGTCTC 1 cut(s) 65
BsaJI CCNNGG 4 cut(s) 42, 496, 517, 854
BsaWI WCCGGW 1 cut(s) 790
BsaXI ACNNNNNCTCC 4 cut(s) 104, 134, 767, 797
Bsc4I CCNNNNNNNGG 2 cut(s) 551, 907
Bse118I RCCGGY 1 cut(s) 907
Bse1I ACTGG 1 cut(s) 863
Bse3DI GCAATG 1 cut(s) 661
BseDI CCNNGG 4 cut(s) 42, 496, 517, 854
BseGI GGATG 2 cut(s) 338, 733
BseLI CCNNNNNNNGG 2 cut(s) 551, 907
BseMI GCAATG 1 cut(s) 661
BseMII CTCAG 2 cut(s) 21, 800
BseNI ACTGG 1 cut(s) 863
BseRI GAGGAG 3 cut(s) 125, 733, 973
BseXI GCAGC 9 cut(s) 238, 259, 262, 266, 286, 422, 486, 489, 769
BshNI GGYRCC 2 cut(s) 500, 793
BsiHKAI GWGCWC 1 cut(s) 168
BsiSI CCGG 2 cut(s) 791, 908
BslFI GGGAC 1 cut(s) 50
BslI CCNNNNNNNGG 2 cut(s) 551, 907
BsmAI GTCTC 3 cut(s) 65, 349, 805
BsmBI CGTCTC 1 cut(s) 805
BsmFI GGGAC 1 cut(s) 50
Bso31I GGTCTC 1 cut(s) 65
Bsp119I TTCGAA 2 cut(s) 695, 767
Bsp1286I GDGCHC 1 cut(s) 168
Bsp143I GATC 2 cut(s) 114, 945
Bsp19I CCATGG 1 cut(s) 496
BspACI CCGC 3 cut(s) 311, 755, 818
BspCNI CTCAG 2 cut(s) 20, 801
BspLI GGNNCC 4 cut(s) 502, 795, 860, 978
BspMAI CTGCAG 1 cut(s) 256
BspMI ACCTGC 2 cut(s) 83, 984
BspT104I TTCGAA 2 cut(s) 695, 767
BspT107I GGYRCC 2 cut(s) 500, 793
BspTI CTTAAG 1 cut(s) 833
BspTNI GGTCTC 1 cut(s) 65
BsrDI GCAATG 1 cut(s) 661
BsrFI RCCGGY 1 cut(s) 907
BsrI ACTGG 1 cut(s) 863
BssAI RCCGGY 1 cut(s) 907
BssECI CCNNGG 4 cut(s) 42, 496, 517, 854
BssMI GATC 2 cut(s) 114, 945
BssNI GRCGYC 1 cut(s) 501
BssSI CACGAG 2 cut(s) 279, 890
BssT1I CCWWGG 2 cut(s) 496, 854
Bst2BI CACGAG 2 cut(s) 279, 890
Bst4CI ACNGT 3 cut(s) 170, 193, 731
BstACI GRCGYC 1 cut(s) 501
BstAFI CTTAAG 1 cut(s) 833
BstBI TTCGAA 2 cut(s) 695, 767
BstC8I GCNNGC 4 cut(s) 243, 887, 987, 991
BstDEI CTNAG 2 cut(s) 7, 809
BstDSI CCRYGG 3 cut(s) 42, 496, 517
BstF5I GGATG 2 cut(s) 338, 733
BstH2I RGCGCY 1 cut(s) 504
BstHHI GCGC 1 cut(s) 503
BstKTI GATC 2 cut(s) 117, 948
BstMAI GTCTC 3 cut(s) 65, 349, 805
BstMBI GATC 2 cut(s) 114, 945
BstMWI GCNNNNNNNGC 5 cut(s) 251, 308, 441, 486, 1007
BstPAI GACNNNNGTC 2 cut(s) 77, 729
BstSFI CTRYAG 1 cut(s) 252
BstV1I GCAGC 9 cut(s) 238, 259, 262, 266, 286, 422, 486, 489, 769
BstV2I GAAGAC 1 cut(s) 902
BtgI CCRYGG 3 cut(s) 42, 496, 517
BtrI CACGTC 1 cut(s) 106
BtsCI GGATG 2 cut(s) 338, 733
BtsI GCAGTG 1 cut(s) 330
BtsIMutI CAGTG 4 cut(s) 157, 166, 330, 684
BveI ACCTGC 2 cut(s) 83, 984
Cac8I GCNNGC 4 cut(s) 243, 887, 987, 991
CfoI GCGC 1 cut(s) 503
Cfr10I RCCGGY 1 cut(s) 907
Cfr13I GGNCC 2 cut(s) 134, 858
CseI GACGC 1 cut(s) 67
Csp6I GTAC 1 cut(s) 607
CviAII CATG 3 cut(s) 175, 401, 497
CviQI GTAC 1 cut(s) 607
DdeI CTNAG 2 cut(s) 7, 809
DinI GGCGCC 1 cut(s) 502
DpnI GATC 2 cut(s) 116, 947
DpnII GATC 2 cut(s) 114, 945
Eco130I CCWWGG 2 cut(s) 496, 854
Eco31I GGTCTC 1 cut(s) 65
Eco47I GGWCC 2 cut(s) 134, 858
Eco57I CTGAAG 2 cut(s) 556, 683
EcoO109I RGGNCCY 1 cut(s) 858
EcoRI GAATTC 2 cut(s) 587, 595
EcoT14I CCWWGG 2 cut(s) 496, 854
EcoT22I ATGCAT 1 cut(s) 709
EgeI GGCGCC 1 cut(s) 502
EheI GGCGCC 1 cut(s) 502
ErhI CCWWGG 2 cut(s) 496, 854
Esp3I CGTCTC 1 cut(s) 805
FaeI CATG 3 cut(s) 178, 404, 500
FalI AAGNNNNNCTT 2 cut(s) 770, 802
FaqI GGGAC 1 cut(s) 50
FatI CATG 3 cut(s) 174, 400, 496
FauNDI CATATG 1 cut(s) 703
FbaI TGATCA 1 cut(s) 945
FokI GGATG 2 cut(s) 345, 720
FspBI CTAG 1 cut(s) 855
GlaI GCGC 1 cut(s) 502
GsuI CTGGAG 1 cut(s) 231
HaeII RGCGCY 1 cut(s) 504
HapII CCGG 2 cut(s) 791, 908
HgaI GACGC 1 cut(s) 67
HhaI GCGC 1 cut(s) 503
Hin1I GRCGYC 1 cut(s) 501
Hin1II CATG 3 cut(s) 178, 404, 500
Hin6I GCGC 1 cut(s) 501
HinP1I GCGC 1 cut(s) 501
HindIII AAGCTT 1 cut(s) 987
HinfI GANTC 3 cut(s) 38, 230, 629
HpaII CCGG 2 cut(s) 791, 908
HphI GGTGA 1 cut(s) 727
Hpy188I TCNGA 7 cut(s) 208, 229, 463, 586, 638, 748, 1017
Hpy188III TCNNGA 4 cut(s) 132, 592, 687, 892
Hpy99I CGWCG 3 cut(s) 241, 265, 411
HpyAV CCTTC 4 cut(s) 580, 621, 766, 964
HpyCH4III ACNGT 3 cut(s) 170, 193, 731
HpyCH4IV ACGT 2 cut(s) 105, 119
HpyCH4V TGCA 7 cut(s) 49, 254, 474, 654, 707, 985, 993
HpyF10VI GCNNNNNNNGC 5 cut(s) 251, 308, 441, 486, 1007
HpyF3I CTNAG 2 cut(s) 7, 809
HpySE526I ACGT 2 cut(s) 105, 119
Hsp92I GRCGYC 1 cut(s) 501
Hsp92II CATG 3 cut(s) 178, 404, 500
HspAI GCGC 1 cut(s) 501
KasI GGCGCC 1 cut(s) 500
Ksp22I TGATCA 1 cut(s) 945
Kzo9I GATC 2 cut(s) 114, 945
LmnI GCTCC 6 cut(s) 25, 163, 250, 262, 805, 1007
Lsp1109I GCAGC 9 cut(s) 238, 259, 262, 266, 286, 422, 486, 489, 769
MaeI CTAG 1 cut(s) 855
MaeII ACGT 2 cut(s) 105, 119
MaeIII GTNAC 4 cut(s) 52, 170, 266, 722
MalI GATC 2 cut(s) 116, 947
MboI GATC 2 cut(s) 114, 945
MboII GAAGA 5 cut(s) 530, 676, 781, 790, 907
MfeI CAATTG 1 cut(s) 798
MhlI GDGCHC 1 cut(s) 168
MluCI AATT 5 cut(s) 587, 595, 798, 936, 952
Mly113I GGCGCC 1 cut(s) 501
MlyI GAGTC 1 cut(s) 32
MmeI TCCRAC 1 cut(s) 771
Mph1103I ATGCAT 1 cut(s) 709
MseI TTAA 3 cut(s) 159, 182, 834
MslI CAYNNNNRTG 2 cut(s) 194, 219
MspA1I CMGCKG 1 cut(s) 251
MspCI CTTAAG 1 cut(s) 833
MspI CCGG 2 cut(s) 791, 908
MunI CAATTG 1 cut(s) 798
MwoI GCNNNNNNNGC 5 cut(s) 251, 308, 441, 486, 1007
NarI GGCGCC 1 cut(s) 501
NcoI CCATGG 1 cut(s) 496
NdeI CATATG 1 cut(s) 703
NdeII GATC 2 cut(s) 114, 945
NlaIII CATG 3 cut(s) 178, 404, 500
NlaIV GGNNCC 4 cut(s) 502, 795, 860, 978
NmuCI GTSAC 3 cut(s) 52, 170, 722
NsiI ATGCAT 1 cut(s) 709
NspV TTCGAA 2 cut(s) 695, 767
OliI CACNNNNGTG 1 cut(s) 194
PcsI WCGNNNNNNNCGW 2 cut(s) 233, 692
PfeI GAWTC 2 cut(s) 230, 629
PleI GAGTC 1 cut(s) 32
PluTI GGCGCC 1 cut(s) 504
PpsI GAGTC 1 cut(s) 32
PpuMI RGGWCCY 1 cut(s) 858
PshAI GACNNNNGTC 2 cut(s) 77, 729
Psp5II RGGWCCY 1 cut(s) 858
PspN4I GGNNCC 4 cut(s) 502, 795, 860, 978
PspPI GGNCC 2 cut(s) 134, 858
PspPPI RGGWCCY 1 cut(s) 858
PstI CTGCAG 1 cut(s) 256
PvuII CAGCTG 1 cut(s) 251
RsaI GTAC 1 cut(s) 608
RsaNI GTAC 1 cut(s) 607
RseI CAYNNNNRTG 2 cut(s) 194, 219
SaqAI TTAA 3 cut(s) 159, 182, 834
Sau3AI GATC 2 cut(s) 114, 945
Sau96I GGNCC 2 cut(s) 134, 858
SchI GAGTC 1 cut(s) 32
SduI GDGCHC 1 cut(s) 168
SfcI CTRYAG 1 cut(s) 252
SfoI GGCGCC 1 cut(s) 502
SfuI TTCGAA 2 cut(s) 695, 767
SinI GGWCC 2 cut(s) 134, 858
SmiMI CAYNNNNRTG 2 cut(s) 194, 219
SmlI CTYRAG 1 cut(s) 833
SmoI CTYRAG 1 cut(s) 833
Sse9I AATT 5 cut(s) 587, 595, 798, 936, 952
SsiI CCGC 3 cut(s) 311, 755, 818
SspDI GGCGCC 1 cut(s) 500
SspMI CTAG 1 cut(s) 855
StyI CCWWGG 2 cut(s) 496, 854
TaaI ACNGT 3 cut(s) 170, 193, 731
TaiI ACGT 2 cut(s) 108, 122
TaqI TCGA 6 cut(s) 263, 409, 627, 686, 695, 767
TasI AATT 5 cut(s) 587, 595, 798, 936, 952
TauI GCSGC 1 cut(s) 821
TfiI GAWTC 2 cut(s) 230, 629
Tru1I TTAA 3 cut(s) 159, 182, 834
Tru9I TTAA 3 cut(s) 159, 182, 834
TscAI CASTG 4 cut(s) 157, 173, 330, 684
TseFI GTSAC 3 cut(s) 52, 170, 722
TseI GCWGC 9 cut(s) 251, 254, 272, 275, 299, 435, 474, 477, 757
Tsp45I GTSAC 3 cut(s) 52, 170, 722
TspDTI ATGAA 3 cut(s) 136, 387, 501
TspGWI ACGGA 5 cut(s) 31, 225, 249, 376, 534
TspRI CASTG 4 cut(s) 157, 173, 330, 684
Vha464I CTTAAG 1 cut(s) 833
VpaK11BI GGWCC 2 cut(s) 134, 858
XapI RAATTY 2 cut(s) 587, 595
XmaJI CCTAGG 1 cut(s) 854
XspI CTAG 1 cut(s) 855
Zsp2I ATGCAT 1 cut(s) 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.