pycom10g19370

APO protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0026694
Physical Location & Seq
Forward (+)
22471984 .. 22472376
393 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g19370.1

Sequence Viewer

Length: 393 bp
ATGGATGCATATGAAACTGTTAGGTTGGGGGTGACAAGGCTGATGAAGAAATACACTGTGAAGGCATGCGGGTATTGCTCCGAGGTTCACGTGGGACCTTGGGGTCACAATGCAAAGCTTTGTGGGGAATTCAAGCACCAATGGAGGGATGGGAAGCATGGCTGGCAGGATGCTACCGTGGACGAGGTTTTCCCTCTAAATTATGTATGGCACGTCGAAGACCCCAAAGGGCCTCCACTGAAGGGCGGTGCACTCAGGAAGTTCTACGGGAAGGCTCCGGCAGTGGTAGAGGTTTGCTTGCAGGCAGGTGCACAGATCCCTGAGAAATATAAACCCATGATGAGGCTTGACATTGTAGTCCCAGAAAGTGAGGAGGCACAATTAGTTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APO_RNA-bind

Protein Analysis

131

Amino Acids

14.63

Weight (kDa)

7.69

Isoelectric Point (pI)

29.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APO_RNA-bind PF05634 2 - 108 4.3e-21 APO RNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0026694)

Species Orthologous Gene IDs
malus_domestica MD15G1151800.v1.1
pyrus_communis pycom10g19370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 296
AasI GACNNNNNNGTC 2 cut(s) 102, 356
Acc36I ACCTGC 1 cut(s) 296
AciI CCGC 2 cut(s) 69, 246
AclWI GGATC 1 cut(s) 310
AcsI RAATTY 1 cut(s) 128
AcuI CTGAAG 1 cut(s) 260
AcvI CACGTG 1 cut(s) 91
AfiI CCNNNNNNNGG 3 cut(s) 145, 242, 342
AgsI TTSAA 1 cut(s) 133
AjiI CACGTC 1 cut(s) 214
AluBI AGCT 1 cut(s) 118
AluI AGCT 1 cut(s) 118
Alw21I GWGCWC 2 cut(s) 253, 313
Alw44I GTGCAC 2 cut(s) 249, 309
AlwI GGATC 1 cut(s) 310
AoxI GGCC 1 cut(s) 230
ApaLI GTGCAC 2 cut(s) 249, 309
ApoI RAATTY 1 cut(s) 128
AspS9I GGNCC 2 cut(s) 95, 230
AsuHPI GGTGA 1 cut(s) 43
AvaII GGWCC 1 cut(s) 95
BaeGI GKGCMC 2 cut(s) 253, 313
BbrPI CACGTG 1 cut(s) 91
BbsI GAAGAC 1 cut(s) 225
Bbv12I GWGCWC 2 cut(s) 253, 313
BccI CCATC 1 cut(s) 143
BfuAI ACCTGC 1 cut(s) 296
Bme18I GGWCC 1 cut(s) 95
BmgBI CACGTC 1 cut(s) 214
BmgT120I GGNCC 2 cut(s) 95, 230
BmiI GGNNCC 2 cut(s) 96, 276
BmsI GCATC 1 cut(s) 160
BpiI GAAGAC 1 cut(s) 225
BsaAI YACGTR 1 cut(s) 91
BsaJI CCNNGG 3 cut(s) 81, 98, 177
Bsc4I CCNNNNNNNGG 3 cut(s) 145, 242, 342
BseDI CCNNGG 3 cut(s) 81, 98, 177
BseGI GGATG 3 cut(s) 10, 154, 175
BseLI CCNNNNNNNGG 3 cut(s) 145, 242, 342
BseMII CTCAG 2 cut(s) 268, 312
BseRI GAGGAG 1 cut(s) 386
BseSI GKGCMC 2 cut(s) 253, 313
BshFI GGCC 1 cut(s) 232
BsiHKAI GWGCWC 2 cut(s) 253, 313
BsiSI CCGG 1 cut(s) 278
BslFI GGGAC 2 cut(s) 108, 344
BslI CCNNNNNNNGG 3 cut(s) 145, 242, 342
BsmFI GGGAC 2 cut(s) 108, 344
BsnI GGCC 1 cut(s) 232
Bsp1286I GDGCHC 2 cut(s) 253, 313
Bsp143I GATC 1 cut(s) 315
BspACI CCGC 2 cut(s) 69, 246
BspANI GGCC 1 cut(s) 232
BspCNI CTCAG 2 cut(s) 267, 313
BspLI GGNNCC 2 cut(s) 96, 276
BspMI ACCTGC 1 cut(s) 296
BspPI GGATC 1 cut(s) 310
BssECI CCNNGG 3 cut(s) 81, 98, 177
BssMI GATC 1 cut(s) 315
BssT1I CCWWGG 1 cut(s) 98
Bst4CI ACNGT 3 cut(s) 19, 58, 178
BstBAI YACGTR 1 cut(s) 91
BstC8I GCNNGC 4 cut(s) 67, 164, 299, 303
BstDEI CTNAG 2 cut(s) 254, 321
BstDSI CCRYGG 1 cut(s) 177
BstF5I GGATG 3 cut(s) 10, 154, 175
BstKTI GATC 1 cut(s) 318
BstMBI GATC 1 cut(s) 315
BstMWI GCNNNNNNNGC 2 cut(s) 75, 163
BstNSI RCATGY 1 cut(s) 69
BstSLI GKGCMC 2 cut(s) 253, 313
BstV2I GAAGAC 1 cut(s) 225
BstX2I RGATCY 1 cut(s) 315
BstYI RGATCY 1 cut(s) 315
BsuRI GGCC 1 cut(s) 232
BtgI CCRYGG 1 cut(s) 177
BtrI CACGTC 1 cut(s) 214
BtsCI GGATG 3 cut(s) 10, 154, 175
BtsI GCAGTG 1 cut(s) 288
BtsIMutI CAGTG 3 cut(s) 54, 236, 288
BveI ACCTGC 1 cut(s) 296
Cac8I GCNNGC 4 cut(s) 67, 164, 299, 303
Cfr13I GGNCC 2 cut(s) 95, 230
CviAII CATG 3 cut(s) 66, 158, 337
CviJI RGCY 6 cut(s) 40, 118, 162, 232, 275, 346
CviKI_1 RGCY 6 cut(s) 40, 118, 162, 232, 275, 346
DdeI CTNAG 2 cut(s) 254, 321
DpnI GATC 1 cut(s) 317
DpnII GATC 1 cut(s) 315
DrdI GACNNNNNNGTC 2 cut(s) 102, 356
DseDI GACNNNNNNGTC 2 cut(s) 102, 356
Eco130I CCWWGG 1 cut(s) 98
Eco47I GGWCC 1 cut(s) 95
Eco57I CTGAAG 1 cut(s) 260
Eco72I CACGTG 1 cut(s) 91
EcoO109I RGGNCCY 2 cut(s) 95, 230
EcoRI GAATTC 1 cut(s) 128
EcoT14I CCWWGG 1 cut(s) 98
EcoT22I ATGCAT 1 cut(s) 10
ErhI CCWWGG 1 cut(s) 98
FaeI CATG 3 cut(s) 69, 161, 340
FaiI YATR 8 cut(s) 10, 12, 67, 159, 204, 208, 330, 338
FaqI GGGAC 2 cut(s) 108, 344
FatI CATG 3 cut(s) 65, 157, 336
FauI CCCGC 1 cut(s) 62
FauNDI CATATG 1 cut(s) 10
FokI GGATG 3 cut(s) 17, 161, 182
HaeIII GGCC 1 cut(s) 232
HapII CCGG 1 cut(s) 278
Hin1II CATG 3 cut(s) 69, 161, 340
HindIII AAGCTT 1 cut(s) 116
HpaII CCGG 1 cut(s) 278
HphI GGTGA 1 cut(s) 43
Hpy166II GTNNAC 4 cut(s) 88, 181, 251, 311
Hpy188I TCNGA 1 cut(s) 82
Hpy188III TCNNGA 1 cut(s) 256
Hpy8I GTNNAC 4 cut(s) 88, 181, 251, 311
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 3 cut(s) 55, 235, 265
HpyCH4III ACNGT 3 cut(s) 19, 58, 178
HpyCH4IV ACGT 2 cut(s) 90, 213
HpyCH4V TGCA 5 cut(s) 8, 113, 251, 301, 311
HpyF10VI GCNNNNNNNGC 2 cut(s) 75, 163
HpyF3I CTNAG 2 cut(s) 254, 321
HpySE526I ACGT 2 cut(s) 90, 213
Hsp92II CATG 3 cut(s) 69, 161, 340
Kzo9I GATC 1 cut(s) 315
LmnI GCTCC 2 cut(s) 83, 280
LpnPI CCDG 8 cut(s) 148, 152, 241, 287, 291, 291, 333, 375
LweI GCATC 1 cut(s) 160
MaeII ACGT 2 cut(s) 90, 213
MaeIII GTNAC 2 cut(s) 31, 104
MalI GATC 1 cut(s) 317
MboI GATC 1 cut(s) 315
MboII GAAGA 2 cut(s) 58, 230
MflI RGATCY 1 cut(s) 315
MhlI GDGCHC 2 cut(s) 253, 313
MluCI AATT 3 cut(s) 128, 199, 380
MnlI CCTC 9 cut(s) 76, 138, 178, 204, 243, 283, 336, 364, 367
Mph1103I ATGCAT 1 cut(s) 10
MspI CCGG 1 cut(s) 278
MwoI GCNNNNNNNGC 2 cut(s) 75, 163
NdeI CATATG 1 cut(s) 10
NdeII GATC 1 cut(s) 315
NlaIII CATG 3 cut(s) 69, 161, 340
NlaIV GGNNCC 2 cut(s) 96, 276
NmuCI GTSAC 2 cut(s) 31, 104
NsiI ATGCAT 1 cut(s) 10
NspI RCATGY 1 cut(s) 69
PaeI GCATGC 1 cut(s) 69
PaqCI CACCTGC 1 cut(s) 296
PmaCI CACGTG 1 cut(s) 91
PmlI CACGTG 1 cut(s) 91
Ppu21I YACGTR 1 cut(s) 91
PpuMI RGGWCCY 1 cut(s) 95
Psp5II RGGWCCY 1 cut(s) 95
PspCI CACGTG 1 cut(s) 91
PspN4I GGNNCC 2 cut(s) 96, 276
PspPI GGNCC 2 cut(s) 95, 230
PspPPI RGGWCCY 1 cut(s) 95
PsuI RGATCY 1 cut(s) 315
Sau3AI GATC 1 cut(s) 315
Sau96I GGNCC 2 cut(s) 95, 230
SduI GDGCHC 2 cut(s) 253, 313
SetI ASST 9 cut(s) 26, 87, 93, 100, 120, 189, 216, 294, 310
SfaNI GCATC 1 cut(s) 160
SinI GGWCC 1 cut(s) 95
SphI GCATGC 1 cut(s) 69
Sse9I AATT 3 cut(s) 128, 199, 380
SsiI CCGC 2 cut(s) 69, 246
StyI CCWWGG 1 cut(s) 98
TaaI ACNGT 3 cut(s) 19, 58, 178
TaiI ACGT 2 cut(s) 93, 216
TaqI TCGA 1 cut(s) 216
TasI AATT 3 cut(s) 128, 199, 380
TscAI CASTG 3 cut(s) 61, 243, 288
TseFI GTSAC 2 cut(s) 31, 104
Tsp45I GTSAC 2 cut(s) 31, 104
TspDTI ATGAA 2 cut(s) 27, 59
TspRI CASTG 3 cut(s) 61, 243, 288
VneI GTGCAC 2 cut(s) 249, 309
VpaK11BI GGWCC 1 cut(s) 95
XapI RAATTY 1 cut(s) 128
XceI RCATGY 1 cut(s) 69
XcmI CCANNNNNNNNNTGG 1 cut(s) 146
Zsp2I ATGCAT 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.