MD15G1152800.v1.1

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0028402
Physical Location & Seq
Forward (+)
11320861 .. 11321679
819 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1152800.v1.1.491

Sequence Viewer

Length: 684 bp
ATGGATGCCGCAGCTTCTTCGTGTTGCAGCTCTGCCCTCCAATCTCTCTTCCAAGCAATGACTCTAAAATCTAGCCAATCCAGATCCATATACCTCGAGATTTCAGAAGCTCAGAATTGTACGAACATATTCGAAAATCTTCACAACAGGATCAATCTTAACAAATGTGAGCTCCAAGACTTTATCTCCTCTTCAACACGGGACCTGTGTTCGAACAATGTGGATCAAATTATTGATCTTATCGGGGTTGAAACGTATAACGAGTTACGCTTCAATTGTAAGGACTTATCTAGCAGGAATCACCGCGATGAAGTGTGTTTCGATTGCGTTGTTTCTTACAGGAAAACCTTGCAAGTGCTAAAGGAAAGAAATAGAGCAAGTGACAAAAGGTGTGCTGAAGCTCTACTTGTTAGTCTAGCTAGCTCAGATGTTCAATCCACAAACTTTGTTCAGGGAACTTTTTCGTGTTTGTGGAACGAGATTCAGTCTCCATGGCCTACACAAAAAAGTCCAAACAAAGCTTATATCTTGGGATCCAAGAAACTCCTTGCCATAGTGATCGTGGCAGTGGCGCTGGTGATTTTAACTCCGATTCTTTACAAGATTACCAGGAAGCAACTAAAATATGCAAGCGAAAAAGATATCGAAATCCTATCCGGTGAGCATTCTGTTAAAATTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase_Tyr

Protein Analysis

228

Amino Acids

25.53

Weight (kDa)

8.0

Isoelectric Point (pI)

55.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028402)

Species Orthologous Gene IDs
malus_domestica MD15G1152800.v1.1
prunus_persica Prupe.7G261800_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 306
AciI CCGC 2 cut(s) 9, 304
AclWI GGATC 5 cut(s) 78, 158, 231, 528, 541
AcuI CTGAAG 1 cut(s) 417
AfaI GTAC 1 cut(s) 121
AgsI TTSAA 4 cut(s) 195, 251, 274, 434
AjnI CCWGG 1 cut(s) 608
AluBI AGCT 8 cut(s) 14, 30, 110, 172, 401, 419, 423, 521
AluI AGCT 8 cut(s) 14, 30, 110, 172, 401, 419, 423, 521
Alw21I GWGCWC 1 cut(s) 174
Alw26I GTCTC 1 cut(s) 492
AlwI GGATC 5 cut(s) 78, 158, 231, 528, 541
Ama87I CYCGRG 1 cut(s) 95
AoxI GGCC 1 cut(s) 494
ApeKI GCWGC 2 cut(s) 11, 27
AseI ATTAAT 1 cut(s) 678
Asp700I GAANNNNTTC 3 cut(s) 128, 138, 460
AspLEI GCGC 1 cut(s) 574
AspS9I GGNCC 1 cut(s) 202
AsuHPI GGTGA 3 cut(s) 293, 589, 671
AsuII TTCGAA 2 cut(s) 132, 212
AsuNHI GCTAGC 1 cut(s) 419
AvaI CYCGRG 1 cut(s) 95
AvaII GGWCC 1 cut(s) 202
BamHI GGATCC 1 cut(s) 533
BanII GRGCYC 1 cut(s) 174
Bbv12I GWGCWC 1 cut(s) 174
BbvI GCAGC 2 cut(s) 23, 39
BcgI CGANNNNNNTGC 1 cut(s) 34
BciT130I CCWGG 1 cut(s) 610
BcoDI GTCTC 1 cut(s) 492
BfaI CTAG 4 cut(s) 72, 291, 416, 420
BfoI RGCGCY 1 cut(s) 575
BisI GCNGC 3 cut(s) 9, 12, 28
BlsI GCNGC 3 cut(s) 10, 13, 29
Bme1390I CCNGG 1 cut(s) 610
Bme18I GGWCC 1 cut(s) 202
BmeT110I CYCGRG 1 cut(s) 95
BmgT120I GGNCC 1 cut(s) 202
BmiI GGNNCC 2 cut(s) 203, 535
BmrFI CCNGG 1 cut(s) 610
BmtI GCTAGC 1 cut(s) 423
Bpu14I TTCGAA 2 cut(s) 132, 212
BsaJI CCNNGG 1 cut(s) 491
BsaWI WCCGGW 1 cut(s) 656
BsaXI ACNNNNNCTCC 2 cut(s) 170, 200
Bse3DI GCAATG 1 cut(s) 63
BseBI CCWGG 1 cut(s) 610
BseDI CCNNGG 1 cut(s) 491
BseGI GGATG 1 cut(s) 10
BseMI GCAATG 1 cut(s) 63
BseMII CTCAG 2 cut(s) 125, 438
BseRI GAGGAG 1 cut(s) 178
BseXI GCAGC 2 cut(s) 23, 39
Bsh1236I CGCG 1 cut(s) 306
BshFI GGCC 1 cut(s) 496
BsiHKAI GWGCWC 1 cut(s) 174
BsiHKCI CYCGRG 1 cut(s) 95
BsiSI CCGG 1 cut(s) 657
BslFI GGGAC 1 cut(s) 215
BsmAI GTCTC 1 cut(s) 492
BsmFI GGGAC 1 cut(s) 215
BsmI GAATGC 1 cut(s) 664
BsnI GGCC 1 cut(s) 496
BsoBI CYCGRG 1 cut(s) 95
Bsp119I TTCGAA 2 cut(s) 132, 212
Bsp1286I GDGCHC 1 cut(s) 174
Bsp143I GATC 6 cut(s) 83, 150, 223, 235, 533, 558
Bsp19I CCATGG 1 cut(s) 491
BspACI CCGC 2 cut(s) 9, 304
BspANI GGCC 1 cut(s) 496
BspCNI CTCAG 2 cut(s) 124, 437
BspFNI CGCG 1 cut(s) 306
BspLI GGNNCC 2 cut(s) 203, 535
BspOI GCTAGC 1 cut(s) 423
BspPI GGATC 5 cut(s) 78, 158, 231, 528, 541
BspT104I TTCGAA 2 cut(s) 132, 212
BsrDI GCAATG 1 cut(s) 63
BssECI CCNNGG 1 cut(s) 491
BssMI GATC 6 cut(s) 83, 150, 223, 235, 533, 558
BssT1I CCWWGG 1 cut(s) 491
Bst2UI CCWGG 1 cut(s) 610
Bst6I CTCTTC 2 cut(s) 53, 196
BstBI TTCGAA 2 cut(s) 132, 212
BstC8I GCNNGC 2 cut(s) 421, 631
BstDEI CTNAG 2 cut(s) 111, 424
BstDSI CCRYGG 1 cut(s) 491
BstF5I GGATG 1 cut(s) 10
BstFNI CGCG 1 cut(s) 306
BstH2I RGCGCY 1 cut(s) 575
BstHHI GCGC 1 cut(s) 574
BstKTI GATC 6 cut(s) 86, 153, 226, 238, 536, 561
BstMAI GTCTC 1 cut(s) 492
BstMBI GATC 6 cut(s) 83, 150, 223, 235, 533, 558
BstNI CCWGG 1 cut(s) 610
BstSCI CCNGG 1 cut(s) 608
BstUI CGCG 1 cut(s) 306
BstV1I GCAGC 2 cut(s) 23, 39
BstX2I RGATCY 2 cut(s) 83, 533
BstYI RGATCY 2 cut(s) 83, 533
BsuRI GGCC 1 cut(s) 496
BtgI CCRYGG 1 cut(s) 491
BtgZI GCGATG 1 cut(s) 321
BtsCI GGATG 1 cut(s) 10
BtsI GCAGTG 1 cut(s) 573
BtsIMutI CAGTG 1 cut(s) 573
Cac8I GCNNGC 2 cut(s) 421, 631
CfoI GCGC 1 cut(s) 574
Cfr13I GGNCC 1 cut(s) 202
Csp6I GTAC 1 cut(s) 120
CspCI CAANNNNNGTGG 2 cut(s) 427, 462
CviAII CATG 1 cut(s) 492
CviQI GTAC 1 cut(s) 120
DdeI CTNAG 2 cut(s) 111, 424
DpnI GATC 6 cut(s) 85, 152, 225, 237, 535, 560
DpnII GATC 6 cut(s) 83, 150, 223, 235, 533, 558
Eam1104I CTCTTC 2 cut(s) 53, 196
EarI CTCTTC 2 cut(s) 53, 196
Ecl136II GAGCTC 1 cut(s) 172
Eco130I CCWWGG 1 cut(s) 491
Eco24I GRGCYC 1 cut(s) 174
Eco32I GATATC 1 cut(s) 643
Eco47I GGWCC 1 cut(s) 202
Eco53kI GAGCTC 1 cut(s) 172
Eco57I CTGAAG 1 cut(s) 417
Eco88I CYCGRG 1 cut(s) 95
EcoICRI GAGCTC 1 cut(s) 172
EcoO109I RGGNCCY 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 608
EcoRV GATATC 1 cut(s) 643
EcoT14I CCWWGG 1 cut(s) 491
EcoT38I GRGCYC 1 cut(s) 174
ErhI CCWWGG 1 cut(s) 491
FaeI CATG 1 cut(s) 495
FaiI YATR 8 cut(s) 89, 91, 128, 258, 493, 525, 554, 627
FalI AAGNNNNNCTT 2 cut(s) 390, 422
FaqI GGGAC 1 cut(s) 215
FatI CATG 1 cut(s) 491
Fnu4HI GCNGC 3 cut(s) 9, 12, 28
FokI GGATG 1 cut(s) 17
FriOI GRGCYC 1 cut(s) 174
Fsp4HI GCNGC 3 cut(s) 9, 12, 28
FspBI CTAG 4 cut(s) 72, 291, 416, 420
GlaI GCGC 1 cut(s) 573
GluI GCNGC 3 cut(s) 9, 12, 28
HaeII RGCGCY 1 cut(s) 575
HaeIII GGCC 1 cut(s) 496
HapII CCGG 1 cut(s) 657
HhaI GCGC 1 cut(s) 574
Hin1II CATG 1 cut(s) 495
Hin6I GCGC 1 cut(s) 572
HinP1I GCGC 1 cut(s) 572
HindIII AAGCTT 1 cut(s) 519
HinfI GANTC 4 cut(s) 61, 298, 481, 592
HpaII CCGG 1 cut(s) 657
HphI GGTGA 3 cut(s) 293, 589, 671
Hpy188I TCNGA 4 cut(s) 106, 114, 427, 591
Hpy188III TCNNGA 2 cut(s) 81, 97
HpyCH4IV ACGT 1 cut(s) 254
HpyCH4V TGCA 3 cut(s) 27, 352, 629
HpyF3I CTNAG 2 cut(s) 111, 424
HpySE526I ACGT 1 cut(s) 254
Hsp92II CATG 1 cut(s) 495
HspAI GCGC 1 cut(s) 572
Kzo9I GATC 6 cut(s) 83, 150, 223, 235, 533, 558
LmnI GCTCC 1 cut(s) 177
Lsp1109I GCAGC 2 cut(s) 23, 39
MaeI CTAG 4 cut(s) 72, 291, 416, 420
MaeII ACGT 1 cut(s) 254
MaeIII GTNAC 2 cut(s) 264, 380
MalI GATC 6 cut(s) 85, 152, 225, 237, 535, 560
MboI GATC 6 cut(s) 83, 150, 223, 235, 533, 558
MboII GAAGA 4 cut(s) 9, 40, 131, 183
MfeI CAATTG 1 cut(s) 274
MflI RGATCY 2 cut(s) 83, 533
MhlI GDGCHC 1 cut(s) 174
MluCI AATT 5 cut(s) 115, 228, 274, 675, 679
MlyI GAGTC 1 cut(s) 55
MnlI CCTC 3 cut(s) 47, 104, 199
MroXI GAANNNNTTC 3 cut(s) 128, 138, 460
MseI TTAA 4 cut(s) 159, 584, 672, 678
MslI CAYNNNNRTG 1 cut(s) 306
MspI CCGG 1 cut(s) 657
MspR9I CCNGG 1 cut(s) 610
MunI CAATTG 1 cut(s) 274
Mva1269I GAATGC 1 cut(s) 664
MvaI CCWGG 1 cut(s) 610
MvnI CGCG 1 cut(s) 306
NcoI CCATGG 1 cut(s) 491
NdeII GATC 6 cut(s) 83, 150, 223, 235, 533, 558
NheI GCTAGC 1 cut(s) 419
NlaIII CATG 1 cut(s) 495
NlaIV GGNNCC 2 cut(s) 203, 535
NmuCI GTSAC 1 cut(s) 380
NspV TTCGAA 2 cut(s) 132, 212
PaeR7I CTCGAG 1 cut(s) 95
PctI GAATGC 1 cut(s) 664
PdmI GAANNNNTTC 3 cut(s) 128, 138, 460
PfeI GAWTC 3 cut(s) 298, 481, 592
PkrI GCNGC 3 cut(s) 10, 13, 29
PleI GAGTC 1 cut(s) 55
PpsI GAGTC 1 cut(s) 55
PpuMI RGGWCCY 1 cut(s) 202
PshBI ATTAAT 1 cut(s) 678
Psp124BI GAGCTC 1 cut(s) 174
Psp5II RGGWCCY 1 cut(s) 202
Psp6I CCWGG 1 cut(s) 608
PspGI CCWGG 1 cut(s) 608
PspN4I GGNNCC 2 cut(s) 203, 535
PspPI GGNCC 1 cut(s) 202
PspPPI RGGWCCY 1 cut(s) 202
PsuI RGATCY 2 cut(s) 83, 533
RsaI GTAC 1 cut(s) 121
RsaNI GTAC 1 cut(s) 120
RseI CAYNNNNRTG 1 cut(s) 306
SacI GAGCTC 1 cut(s) 174
SaqAI TTAA 4 cut(s) 159, 584, 672, 678
SatI GCNGC 3 cut(s) 9, 12, 28
Sau3AI GATC 6 cut(s) 83, 150, 223, 235, 533, 558
Sau96I GGNCC 1 cut(s) 202
SchI GAGTC 1 cut(s) 55
ScrFI CCNGG 1 cut(s) 610
SduI GDGCHC 1 cut(s) 174
Sfr274I CTCGAG 1 cut(s) 95
SfuI TTCGAA 2 cut(s) 132, 212
SinI GGWCC 1 cut(s) 202
SlaI CTCGAG 1 cut(s) 95
SmiMI CAYNNNNRTG 1 cut(s) 306
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
Sse9I AATT 5 cut(s) 115, 228, 274, 675, 679
SsiI CCGC 2 cut(s) 9, 304
SspMI CTAG 4 cut(s) 72, 291, 416, 420
SstI GAGCTC 1 cut(s) 174
StyD4I CCNGG 1 cut(s) 608
StyI CCWWGG 1 cut(s) 491
TaiI ACGT 1 cut(s) 257
TaqI TCGA 5 cut(s) 96, 132, 212, 321, 645
TasI AATT 5 cut(s) 115, 228, 274, 675, 679
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 3 cut(s) 298, 481, 592
Tru1I TTAA 4 cut(s) 159, 584, 672, 678
Tru9I TTAA 4 cut(s) 159, 584, 672, 678
TscAI CASTG 1 cut(s) 573
TseFI GTSAC 1 cut(s) 380
TseI GCWGC 2 cut(s) 11, 27
Tsp45I GTSAC 1 cut(s) 380
TspDTI ATGAA 1 cut(s) 324
TspRI CASTG 1 cut(s) 573
VpaK11BI GGWCC 1 cut(s) 202
VspI ATTAAT 1 cut(s) 678
XcmI CCANNNNNNNNNTGG 1 cut(s) 559
XhoI CTCGAG 1 cut(s) 95
XmnI GAANNNNTTC 3 cut(s) 128, 138, 460
XspI CTAG 4 cut(s) 72, 291, 416, 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.