Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0013485

Physical Location & Seq

Genomic Coordinates Reverse (-)

11745420 .. 11748695

Gene Structure 3276 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1158200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1752 bp

ATGAAGACTCCGCAGGGTAAGAATTCCCATACAGAGATCCAAGTGGGACATTATTTTTTTCATCTCAGTCAAGTCAACTCTTTGAAAATATCCAAGTGTGCCGGTGATATTAATTTCGTTCTTTCCTCCCAGAAAGTTGAGAGTTTCTTTTTTTCTTCTTCCGTGACATACGAGCTAGCTCATCTCTGTTTGGAAATGGCGGGCGCTTCTTTCGCACCGGCCGGCGTGGCCAAGGAGAGAGCAGAGCAGTACCAAGGGAGGCTAACCCTTTATGTCATCGTTGCCTGCATAGTTGCCGCCGTTGGCGGCTCGCTTTTCGGTTATGATATCGGAATTTCAGGCGGGGTTACGTCAATGGATGGATTTCTTAAGAAATTCTTCAAGACAGTGTACAAGAACAAAACGCATGCCCGGGAAAACAACTACTGCAAGTATGATAACCAGGGTCTTGCAGCGTTTACCTCTTCGCTGTACCTTGCGGGTTTAGTTGCATCTTTAGTGGCTTCTCCGGTCACAAGAAATTATGGACGCCGAGGAAGTATAATCTGTGGCGGAATCAGTTTCCTCGTTGGAGCAACTCTAAACGCTTCAGCAGCGAATTTGGCCATGCTTATCTTAGGTCGGATCATGCTTGGTGTTGGTATCGGATTTGGAAATCAGGCTATTCCGCTATATCTATCAGAAATGGCGCCAACGCATCTTCGAGGAGCCTTAAACATGATGTTTCAGTTAGCAACTACGCTTGGGATCTTCACGGCAAACATGGTAAACTACGGAACTCAAAAGCTCGAACCATGGGGGTGGAGGCTCTCGCTAGGGCTGGCTCTAGTGCCGGCTGCAGTAATGACAGTTGGAGGAATATGTCTGCCCGAGACACCAAATAGCTTGATTGAACGAGGATGTAAAGACGAAGGAAGAAAAATTTTGGAGAGAATCAGAGGAACCAAAAACGTCAGTGCAGAGTTCCAGGACATGTTGGATGCAAGTGAGTTTGCCAGTGCAATAAAGCACCCGTTCAGAAACGTCCTCGAGCGAAGGAACAGGCCTCAGCTCGTTATGGCAATCTTCATGCCGACATTCCAGATCCTCACGGGGATAAATTCGATTCTGTTCTACGCTCCCGTGTTGTTTCAAAGTATGGGGTTCGGGGGAAATGCTGCTTTGTACTCATCTGCTTTAACCGGAGCGGTTCTTGTTACGTCTACACTGCTATCTATCGCAGCAGTTGACAAGCTGGGACGAAGAGTTCTGCTCATTACTGGTGGAATACAAATGATTGTATGCCAGGTCATAGTCGCAGTAATCTTGGGGACCAAGTTAGGGGACAATCAAGAACTATCTAAAGGATTCTCAGTACTGGTGGTGGCGGTGATTTGCCTCTTCGTGATAGCATTCGGATATTCATGGGGTCCCCTCGGCTGGACAGTGCCAAGCGAGATATTCCCGCTAGAAACTCGATCAGCTGGACAAAGCATTACGGTGTCAGTGAACCTTCTGTTCACTTTCATCATCGCGCAGTCGTTTCTCTCCCTCCTGTGCGCGCTCAAATTCGGTATCTTTCTCTTCTTTGCGGGGTGGATTACTATCATGACCGTGTTTGTTTACCTGTTCCTGCCCGAAACCAAGGGAATTCCGATCGAAGAGATGATACTGATGTGGAGAAAGCACTGGTTCTGGAAGAGGATAATGCCTGAAGAATACCTTGAAGCTGATGACTCTGTTGGTGTTAGGCAAAACAATTCAACAGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005351 GO:0005355 GO:0005402 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0008643 GO:0008645 GO:0012505 GO:0015075 GO:0015077 GO:0015078 GO:0015144 GO:0015145 GO:0015146 GO:0015149 GO:0015291 GO:0015293 GO:0015294 GO:0015295 GO:0015318 GO:0015672 GO:0015749 GO:0015750 GO:0015751 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0034219 GO:0034220 GO:0042882 GO:0042900 GO:0042995 GO:0044464 GO:0046323 GO:0051119 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071944 GO:0090406 GO:0098655 GO:0098660 GO:0098662 GO:0120025 GO:1902600 GO:1904659

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sugar_tr

Protein Analysis

584

Amino Acids

63.61

Weight (kDa)

9.09

Isoelectric Point (pI)

33.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sugar_tr	PF00083	96 - 553	1e-118	Sugar (and other) transporter
MFS_1	PF07690	153 - 502	1.3e-22	Major Facilitator Superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013485)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02050 AT4G02050
fragaria_vesca	FvH4_1g01560
malus_domestica	MD02G1012400.v1.1 MD15G1158200.v1.1
prunus_persica	Prupe.7G253400_v2.0.a1 Prupe.7G253400_v2.0.a1
pyrus_communis	pycom02g01160 pycom15g14200
rosa_chinensis	RchiOBHm_Chr2g0086521
rosa_laevigata	RLG00000015763
rosa_multiflora	Rmu_sc0012559.1_g000001
rosa_roxburghii	Rroxscaffold_2G00154480
rosa_rugosa	Rorug01G0467700
rosa_samantha	Rh2BG018000 Rh2CG018900 Rh2DG019800
rosa_wichuraiana	Rw2G001550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	688
AccBSI	CCGCTC	1 cut(s)	1187
AccI	GTMKAC	1 cut(s)	1202
AccII	CGCG	2 cut(s)	1514, 1541
AclWI	GGATC	4 cut(s)	31, 632, 755, 1078
AcoI	YGGCCR	3 cut(s)	219, 228, 603
AcsI	RAATTY	8 cut(s)	22, 333, 374, 598, 921, 1099, 1547, 1629
AcuI	CTGAAG	2 cut(s)	573, 1713
AcyI	GRCGYC	2 cut(s)	529, 689
AfaI	GTAC	5 cut(s)	251, 392, 473, 1166, 1356
AfiI	CCNNNNNNNGG	2 cut(s)	1320, 1419
AflII	CTTAAG	1 cut(s)	368
AflIII	ACRYGT	1 cut(s)	972
AgsI	TTSAA	6 cut(s)	85, 382, 893, 1133, 1706, 1743
AjnI	CCWGG	3 cut(s)	441, 966, 1284
AloI	GAACNNNNNNTCC	2 cut(s)	1230, 1262
AluBI	AGCT	8 cut(s)	175, 179, 787, 885, 1051, 1234, 1463, 1709
AluI	AGCT	8 cut(s)	175, 179, 787, 885, 1051, 1234, 1463, 1709
Alw26I	GTCTC	1 cut(s)	866
AlwI	GGATC	4 cut(s)	31, 632, 755, 1078
Ama87I	CYCGRG	3 cut(s)	411, 869, 1028
AoxI	GGCC	4 cut(s)	219, 228, 603, 1043
ApeKI	GCWGC	5 cut(s)	452, 593, 836, 1157, 1220
ApoI	RAATTY	8 cut(s)	22, 333, 374, 598, 921, 1099, 1547, 1629
AseI	ATTAAT	1 cut(s)	111
Asp700I	GAANNNNTTC	1 cut(s)	377
AspLEI	GCGC	5 cut(s)	206, 691, 1516, 1541, 1543
AspS9I	GGNCC	2 cut(s)	1311, 1409
AsuC2I	CCSGG	2 cut(s)	412, 413
AsuHPI	GGTGA	2 cut(s)	116, 1381
AsuNHI	GCTAGC	1 cut(s)	175
AvaI	CYCGRG	3 cut(s)	411, 869, 1028
AvaII	GGWCC	2 cut(s)	1311, 1409
BaeI	ACNNNNGTAYC	2 cut(s)	1640, 1673
BalI	TGGCCA	2 cut(s)	230, 605
BanI	GGYRCC	1 cut(s)	688
BarI	GAAGNNNNNNTAC	2 cut(s)	1632, 1664
BbsI	GAAGAC	1 cut(s)	11
BbvCI	CCTCAGC	1 cut(s)	1047
BbvI	GCAGC	5 cut(s)	464, 605, 823, 1144, 1232
BccI	CCATC	1 cut(s)	353
BceAI	ACGGC	2 cut(s)	284, 771
BciT130I	CCWGG	3 cut(s)	443, 968, 1286
BcnI	CCSGG	2 cut(s)	412, 413
BcoDI	GTCTC	1 cut(s)	866
BfaI	CTAG	4 cut(s)	176, 815, 827, 1448
BfmI	CTRYAG	1 cut(s)	837
BfoI	RGCGCY	2 cut(s)	207, 692
BfrI	CTTAAG	1 cut(s)	368
BglI	GCCNNNNNGGC	1 cut(s)	227
BisI	GCNGC	7 cut(s)	297, 307, 453, 594, 837, 1158, 1221
BlsI	GCNGC	7 cut(s)	298, 308, 454, 595, 838, 1159, 1222
BmcAI	AGTACT	1 cut(s)	1356
Bme1390I	CCNGG	5 cut(s)	412, 413, 443, 968, 1286
Bme18I	GGWCC	2 cut(s)	1311, 1409
BmeT110I	CYCGRG	3 cut(s)	411, 869, 1028
BmgT120I	GGNCC	2 cut(s)	1311, 1409
BmiI	GGNNCC	6 cut(s)	690, 709, 943, 1312, 1410, 1411
BmrFI	CCNGG	5 cut(s)	412, 413, 443, 968, 1286
BmsI	GCATC	3 cut(s)	500, 706, 970
BmtI	GCTAGC	1 cut(s)	179
BpiI	GAAGAC	1 cut(s)	11
BplI	GAGNNNNNCTC	2 cut(s)	1236, 1268
Bpu10I	CCTNAGC	1 cut(s)	1047
BpuMI	CCSGG	2 cut(s)	412, 413
BsaBI	GATNNNNATC	1 cut(s)	1583
BsaHI	GRCGYC	2 cut(s)	529, 689
BsaJI	CCNNGG	8 cut(s)	231, 253, 411, 442, 532, 794, 1414, 1623
BsaWI	WCCGGW	2 cut(s)	508, 1181
Bsc4I	CCNNNNNNNGG	2 cut(s)	1320, 1419
Bse118I	RCCGGY	4 cut(s)	101, 217, 221, 832
Bse1I	ACTGG	4 cut(s)	996, 1264, 1362, 1673
Bse8I	GATNNNNATC	1 cut(s)	1583
BseBI	CCWGG	3 cut(s)	443, 968, 1286
BseDI	CCNNGG	8 cut(s)	231, 253, 411, 442, 532, 794, 1414, 1623
BseGI	GGATG	3 cut(s)	364, 905, 985
BseJI	GATNNNNATC	1 cut(s)	1583
BseLI	CCNNNNNNNGG	2 cut(s)	1320, 1419
BseMII	CTCAG	3 cut(s)	79, 1061, 1365
BseNI	ACTGG	4 cut(s)	996, 1264, 1362, 1673
BsePI	GCGCGC	1 cut(s)	1539
BseRI	GAGGAG	1 cut(s)	720
BseX3I	CGGCCG	1 cut(s)	219
BseXI	GCAGC	5 cut(s)	464, 605, 823, 1144, 1232
BseYI	CCCAGC	1 cut(s)	1234
BsgI	GTGCAG	1 cut(s)	978
Bsh1236I	CGCG	2 cut(s)	1514, 1541
Bsh1285I	CGRYCG	2 cut(s)	222, 1638
BshFI	GGCC	4 cut(s)	221, 230, 605, 1045
BshNI	GGYRCC	1 cut(s)	688
BsiEI	CGRYCG	2 cut(s)	222, 1638
BsiHKCI	CYCGRG	3 cut(s)	411, 869, 1028
BsiSI	CCGG	7 cut(s)	102, 218, 222, 412, 509, 833, 1182
BslFI	GGGAC	5 cut(s)	60, 1251, 1324, 1337, 1395
BslI	CCNNNNNNNGG	2 cut(s)	1320, 1419
BsmAI	GTCTC	1 cut(s)	866
BsmFI	GGGAC	5 cut(s)	60, 1251, 1324, 1337, 1395
BsmI	GAATGC	1 cut(s)	1391
BsnI	GGCC	4 cut(s)	221, 230, 605, 1045
BsoBI	CYCGRG	3 cut(s)	411, 869, 1028
Bsp1407I	TGTACA	1 cut(s)	390
Bsp143I	GATC	6 cut(s)	36, 624, 747, 1083, 1457, 1635
Bsp19I	CCATGG	1 cut(s)	794
BspANI	GGCC	4 cut(s)	221, 230, 605, 1045
BspCNI	CTCAG	3 cut(s)	78, 1060, 1364
BspFNI	CGCG	2 cut(s)	1514, 1541
BspHI	TCATGA	1 cut(s)	1587
BspLI	GGNNCC	6 cut(s)	690, 709, 943, 1312, 1410, 1411
BspMAI	CTGCAG	1 cut(s)	841
BspOI	GCTAGC	1 cut(s)	179
BspPI	GGATC	4 cut(s)	31, 632, 755, 1078
BspT107I	GGYRCC	1 cut(s)	688
BspTI	CTTAAG	1 cut(s)	368
BsrBI	CCGCTC	1 cut(s)	1187
BsrFI	RCCGGY	4 cut(s)	101, 217, 221, 832
BsrGI	TGTACA	1 cut(s)	390
BsrI	ACTGG	4 cut(s)	996, 1264, 1362, 1673
BssAI	RCCGGY	4 cut(s)	101, 217, 221, 832
BssECI	CCNNGG	8 cut(s)	231, 253, 411, 442, 532, 794, 1414, 1623
BssHII	GCGCGC	1 cut(s)	1539
BssMI	GATC	6 cut(s)	36, 624, 747, 1083, 1457, 1635
BssNI	GRCGYC	2 cut(s)	529, 689
BssT1I	CCWWGG	4 cut(s)	231, 253, 794, 1623
Bst2UI	CCWGG	3 cut(s)	443, 968, 1286
Bst4CI	ACNGT	6 cut(s)	388, 850, 1426, 1480, 1594, 1747
Bst6I	CTCTTC	6 cut(s)	469, 1237, 1385, 1568, 1635, 1673
BstACI	GRCGYC	2 cut(s)	529, 689
BstAFI	CTTAAG	1 cut(s)	368
BstAUI	TGTACA	1 cut(s)	390
BstC8I	GCNNGC	9 cut(s)	177, 202, 223, 286, 311, 408, 822, 834, 1541
BstDEI	CTNAG	4 cut(s)	65, 616, 1047, 1351
BstDSI	CCRYGG	1 cut(s)	794
BstF5I	GGATG	3 cut(s)	364, 905, 985
BstFNI	CGCG	2 cut(s)	1514, 1541
BstH2I	RGCGCY	2 cut(s)	207, 692
BstHHI	GCGC	5 cut(s)	206, 691, 1516, 1541, 1543
BstKTI	GATC	6 cut(s)	39, 627, 750, 1086, 1460, 1638
BstMAI	GTCTC	1 cut(s)	866
BstMBI	GATC	6 cut(s)	36, 624, 747, 1083, 1457, 1635
BstMCI	CGRYCG	2 cut(s)	222, 1638
BstMWI	GCNNNNNNNGC	4 cut(s)	212, 227, 593, 602
BstNI	CCWGG	3 cut(s)	443, 968, 1286
BstNSI	RCATGY	2 cut(s)	410, 976
BstSCI	CCNGG	5 cut(s)	410, 411, 441, 966, 1284
BstSFI	CTRYAG	1 cut(s)	837
BstUI	CGCG	2 cut(s)	1514, 1541
BstV1I	GCAGC	5 cut(s)	464, 605, 823, 1144, 1232
BstV2I	GAAGAC	1 cut(s)	11
BstX2I	RGATCY	3 cut(s)	36, 747, 1083
BstXI	CCANNNNNNTGG	1 cut(s)	801
BstYI	RGATCY	3 cut(s)	36, 747, 1083
BstZI	CGGCCG	1 cut(s)	219
BsuRI	GGCC	4 cut(s)	221, 230, 605, 1045
BtgI	CCRYGG	1 cut(s)	794
BtgZI	GCGATG	1 cut(s)	1495
BtsCI	GGATG	3 cut(s)	364, 905, 985
BtsI	GCAGTG	1 cut(s)	1205
BtsIMutI	CAGTG	7 cut(s)	393, 961, 1003, 1205, 1431, 1491, 1666
Cac8I	GCNNGC	9 cut(s)	177, 202, 223, 286, 311, 408, 822, 834, 1541
CciI	TCATGA	1 cut(s)	1587
CfoI	GCGC	5 cut(s)	206, 691, 1516, 1541, 1543
Cfr10I	RCCGGY	4 cut(s)	101, 217, 221, 832
Cfr13I	GGNCC	2 cut(s)	1311, 1409
Cfr9I	CCCGGG	1 cut(s)	411
CseI	GACGC	1 cut(s)	537
Csp6I	GTAC	5 cut(s)	250, 391, 472, 1165, 1355
CviQI	GTAC	5 cut(s)	250, 391, 472, 1165, 1355
DdeI	CTNAG	4 cut(s)	65, 616, 1047, 1351
DinI	GGCGCC	1 cut(s)	690
DpnI	GATC	6 cut(s)	38, 626, 749, 1085, 1459, 1637
DpnII	GATC	6 cut(s)	36, 624, 747, 1083, 1457, 1635
EaeI	YGGCCR	3 cut(s)	219, 228, 603
EagI	CGGCCG	1 cut(s)	219
Eam1104I	CTCTTC	6 cut(s)	469, 1237, 1385, 1568, 1635, 1673
EarI	CTCTTC	6 cut(s)	469, 1237, 1385, 1568, 1635, 1673
EciI	GGCGGA	1 cut(s)	567
EclXI	CGGCCG	1 cut(s)	219
Eco130I	CCWWGG	4 cut(s)	231, 253, 794, 1623
Eco147I	AGGCCT	1 cut(s)	1045
Eco32I	GATATC	1 cut(s)	328
Eco47I	GGWCC	2 cut(s)	1311, 1409
Eco52I	CGGCCG	1 cut(s)	219
Eco57I	CTGAAG	2 cut(s)	573, 1713
Eco88I	CYCGRG	3 cut(s)	411, 869, 1028
EcoO109I	RGGNCCY	1 cut(s)	1409
EcoRI	GAATTC	2 cut(s)	22, 1629
EcoRII	CCWGG	3 cut(s)	441, 966, 1284
EcoRV	GATATC	1 cut(s)	328
EcoT14I	CCWWGG	4 cut(s)	231, 253, 794, 1623
EgeI	GGCGCC	1 cut(s)	690
EheI	GGCGCC	1 cut(s)	690
ErhI	CCWWGG	4 cut(s)	231, 253, 794, 1623
FalI	AAGNNNNNCTT	4 cut(s)	362, 394, 1686, 1718
FaqI	GGGAC	5 cut(s)	60, 1251, 1324, 1337, 1395
FauI	CCCGC	5 cut(s)	193, 335, 472, 1452, 1564
FblI	GTMKAC	1 cut(s)	1202
Fnu4HI	GCNGC	7 cut(s)	297, 307, 453, 594, 837, 1158, 1221
FokI	GGATG	3 cut(s)	371, 912, 992
Fsp4HI	GCNGC	7 cut(s)	297, 307, 453, 594, 837, 1158, 1221
FspBI	CTAG	4 cut(s)	176, 815, 827, 1448
GlaI	GCGC	5 cut(s)	205, 690, 1515, 1540, 1542
GluI	GCNGC	7 cut(s)	297, 307, 453, 594, 837, 1158, 1221
GsaI	CCCAGC	1 cut(s)	1238
HaeII	RGCGCY	2 cut(s)	207, 692
HaeIII	GGCC	4 cut(s)	221, 230, 605, 1045
HapII	CCGG	7 cut(s)	102, 218, 222, 412, 509, 833, 1182
HgaI	GACGC	1 cut(s)	537
HhaI	GCGC	5 cut(s)	206, 691, 1516, 1541, 1543
Hin1I	GRCGYC	2 cut(s)	529, 689
Hin6I	GCGC	5 cut(s)	204, 689, 1514, 1539, 1541
HinP1I	GCGC	5 cut(s)	204, 689, 1514, 1539, 1541
HincII	GTYRAC	2 cut(s)	76, 1228
HindII	GTYRAC	2 cut(s)	76, 1228
HinfI	GANTC	6 cut(s)	7, 555, 933, 1105, 1347, 1715
HpaII	CCGG	7 cut(s)	102, 218, 222, 412, 509, 833, 1182
HphI	GGTGA	2 cut(s)	116, 1381
Hpy166II	GTNNAC	9 cut(s)	76, 391, 459, 769, 1203, 1228, 1489, 1500, 1603
Hpy188I	TCNGA	8 cut(s)	332, 624, 647, 682, 938, 1019, 1397, 1635
Hpy188III	TCNNGA	6 cut(s)	382, 1081, 1331, 1384, 1588, 1675
Hpy8I	GTNNAC	9 cut(s)	76, 391, 459, 769, 1203, 1228, 1489, 1500, 1603
HpyAV	CCTTC	3 cut(s)	905, 1029, 1502
HpyCH4III	ACNGT	6 cut(s)	388, 850, 1426, 1480, 1594, 1747
HpyCH4IV	ACGT	4 cut(s)	350, 951, 1023, 1199
HpyCH4V	TGCA	8 cut(s)	288, 429, 452, 491, 839, 959, 983, 1001
HpyF10VI	GCNNNNNNNGC	4 cut(s)	212, 227, 593, 602
HpyF3I	CTNAG	4 cut(s)	65, 616, 1047, 1351
HpySE526I	ACGT	4 cut(s)	350, 951, 1023, 1199
Hsp92I	GRCGYC	2 cut(s)	529, 689
HspAI	GCGC	5 cut(s)	204, 689, 1514, 1539, 1541
KasI	GGCGCC	1 cut(s)	688
KflI	GGGWCCC	1 cut(s)	1409
KroI	GCCGGC	2 cut(s)	221, 832
KroNI	GCCGGC	2 cut(s)	223, 834
Kzo9I	GATC	6 cut(s)	36, 624, 747, 1083, 1457, 1635
LmnI	GCTCC	4 cut(s)	572, 707, 1123, 1184
Lsp1109I	GCAGC	5 cut(s)	464, 605, 823, 1144, 1232
LweI	GCATC	3 cut(s)	500, 706, 970
MaeI	CTAG	4 cut(s)	176, 815, 827, 1448
MaeII	ACGT	4 cut(s)	350, 951, 1023, 1199
MaeIII	GTNAC	4 cut(s)	163, 346, 511, 1195
MalI	GATC	6 cut(s)	38, 626, 749, 1085, 1459, 1637
MbiI	CCGCTC	1 cut(s)	1187
MboI	GATC	6 cut(s)	36, 624, 747, 1083, 1457, 1635
MflI	RGATCY	3 cut(s)	36, 747, 1083
MlsI	TGGCCA	2 cut(s)	230, 605
MluNI	TGGCCA	2 cut(s)	230, 605
Mly113I	GGCGCC	1 cut(s)	689
MlyI	GAGTC	1 cut(s)	1709
MmeI	TCCRAC	4 cut(s)	550, 602, 832, 957
Mox20I	TGGCCA	2 cut(s)	230, 605
MroNI	GCCGGC	2 cut(s)	221, 832
MroXI	GAANNNNTTC	1 cut(s)	377
MscI	TGGCCA	2 cut(s)	230, 605
MseI	TTAA	5 cut(s)	111, 369, 713, 1178, 1750
MslI	CAYNNNNRTG	3 cut(s)	799, 1478, 1592
Msp20I	TGGCCA	2 cut(s)	230, 605
MspA1I	CMGCKG	1 cut(s)	1463
MspCI	CTTAAG	1 cut(s)	368
MspI	CCGG	7 cut(s)	102, 218, 222, 412, 509, 833, 1182
MspR9I	CCNGG	5 cut(s)	412, 413, 443, 968, 1286
Mva1269I	GAATGC	1 cut(s)	1391
MvaI	CCWGG	3 cut(s)	443, 968, 1286
MvnI	CGCG	2 cut(s)	1514, 1541
MwoI	GCNNNNNNNGC	4 cut(s)	212, 227, 593, 602
NaeI	GCCGGC	2 cut(s)	223, 834
NarI	GGCGCC	1 cut(s)	689
NciI	CCSGG	2 cut(s)	412, 413
NcoI	CCATGG	1 cut(s)	794
NdeII	GATC	6 cut(s)	36, 624, 747, 1083, 1457, 1635
NgoMIV	GCCGGC	2 cut(s)	221, 832
NheI	GCTAGC	1 cut(s)	175
NlaIV	GGNNCC	6 cut(s)	690, 709, 943, 1312, 1410, 1411
NmeAIII	GCCGAG	2 cut(s)	557, 1395
NmuCI	GTSAC	2 cut(s)	163, 511
NspI	RCATGY	2 cut(s)	410, 976
PaeI	GCATGC	1 cut(s)	410
PaeR7I	CTCGAG	1 cut(s)	1028
PagI	TCATGA	1 cut(s)	1587
PauI	GCGCGC	1 cut(s)	1539
PceI	AGGCCT	1 cut(s)	1045
PciI	ACATGT	1 cut(s)	972
PctI	GAATGC	1 cut(s)	1391
PdiI	GCCGGC	2 cut(s)	223, 834
PdmI	GAANNNNTTC	1 cut(s)	377
PfeI	GAWTC	4 cut(s)	555, 933, 1105, 1347
PfoI	TCCNGGA	1 cut(s)	966
PkrI	GCNGC	7 cut(s)	298, 308, 454, 595, 838, 1159, 1222
Ple19I	CGATCG	1 cut(s)	1638
PleI	GAGTC	1 cut(s)	1709
PluTI	GGCGCC	1 cut(s)	692
PpsI	GAGTC	1 cut(s)	1709
PpuMI	RGGWCCY	1 cut(s)	1409
PscI	ACATGT	1 cut(s)	972
PshBI	ATTAAT	1 cut(s)	111
Psp5II	RGGWCCY	1 cut(s)	1409
Psp6I	CCWGG	3 cut(s)	441, 966, 1284
PspFI	CCCAGC	1 cut(s)	1234
PspGI	CCWGG	3 cut(s)	441, 966, 1284
PspN4I	GGNNCC	6 cut(s)	690, 709, 943, 1312, 1410, 1411
PspPI	GGNCC	2 cut(s)	1311, 1409
PspPPI	RGGWCCY	1 cut(s)	1409
PspXI	VCTCGAGB	1 cut(s)	1028
PstI	CTGCAG	1 cut(s)	841
PsuI	RGATCY	3 cut(s)	36, 747, 1083
PteI	GCGCGC	1 cut(s)	1539
PvuI	CGATCG	1 cut(s)	1638
PvuII	CAGCTG	1 cut(s)	1463
RsaI	GTAC	5 cut(s)	251, 392, 473, 1166, 1356
RsaNI	GTAC	5 cut(s)	250, 391, 472, 1165, 1355
RseI	CAYNNNNRTG	3 cut(s)	799, 1478, 1592
SaqAI	TTAA	5 cut(s)	111, 369, 713, 1178, 1750
SatI	GCNGC	7 cut(s)	297, 307, 453, 594, 837, 1158, 1221
Sau3AI	GATC	6 cut(s)	36, 624, 747, 1083, 1457, 1635
Sau96I	GGNCC	2 cut(s)	1311, 1409
ScaI	AGTACT	1 cut(s)	1356
SchI	GAGTC	1 cut(s)	1709
ScrFI	CCNGG	5 cut(s)	412, 413, 443, 968, 1286
SfaNI	GCATC	3 cut(s)	500, 706, 970
SfcI	CTRYAG	1 cut(s)	837
SfiI	GGCCNNNNNGGCC	1 cut(s)	227
SfoI	GGCGCC	1 cut(s)	690
Sfr274I	CTCGAG	1 cut(s)	1028
SinI	GGWCC	2 cut(s)	1311, 1409
SlaI	CTCGAG	1 cut(s)	1028
SmaI	CCCGGG	1 cut(s)	413
SmiMI	CAYNNNNRTG	3 cut(s)	799, 1478, 1592
SmlI	CTYRAG	2 cut(s)	368, 1028
SmoI	CTYRAG	2 cut(s)	368, 1028
SphI	GCATGC	1 cut(s)	410
SseBI	AGGCCT	1 cut(s)	1045
SspDI	GGCGCC	1 cut(s)	688
SspMI	CTAG	4 cut(s)	176, 815, 827, 1448
StuI	AGGCCT	1 cut(s)	1045
StyD4I	CCNGG	5 cut(s)	410, 411, 441, 966, 1284
StyI	CCWWGG	4 cut(s)	231, 253, 794, 1623
TaaI	ACNGT	6 cut(s)	388, 850, 1426, 1480, 1594, 1747
TaiI	ACGT	4 cut(s)	353, 954, 1026, 1202
TaqI	TCGA	6 cut(s)	703, 789, 1029, 1103, 1456, 1638
TatI	WGTACW	3 cut(s)	390, 1164, 1354
TauI	GCSGC	2 cut(s)	299, 309
TfiI	GAWTC	4 cut(s)	555, 933, 1105, 1347
Tru1I	TTAA	5 cut(s)	111, 369, 713, 1178, 1750
Tru9I	TTAA	5 cut(s)	111, 369, 713, 1178, 1750
TscAI	CASTG	7 cut(s)	393, 961, 1003, 1212, 1431, 1491, 1673
TseFI	GTSAC	2 cut(s)	163, 511
TseI	GCWGC	5 cut(s)	452, 593, 836, 1157, 1220
Tsp45I	GTSAC	2 cut(s)	163, 511
TspDTI	ATGAA	5 cut(s)	17, 50, 1057, 1392, 1495
TspGWI	ACGGA	2 cut(s)	151, 789
TspMI	CCCGGG	1 cut(s)	411
TspRI	CASTG	7 cut(s)	393, 961, 1003, 1212, 1431, 1491, 1673
Vha464I	CTTAAG	1 cut(s)	368
VpaK11BI	GGWCC	2 cut(s)	1311, 1409
VspI	ATTAAT	1 cut(s)	111
XapI	RAATTY	8 cut(s)	22, 333, 374, 598, 921, 1099, 1547, 1629
XceI	RCATGY	2 cut(s)	410, 976
XhoI	CTCGAG	1 cut(s)	1028
XmaI	CCCGGG	1 cut(s)	411
XmiI	GTMKAC	1 cut(s)	1202
XmnI	GAANNNNTTC	1 cut(s)	377
XspI	CTAG	4 cut(s)	176, 815, 827, 1448
ZrmI	AGTACT	1 cut(s)	1356

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.