MD15G1191000.v1.1

Stachyose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0028378
Physical Location & Seq
Reverse (-)
15023044 .. 15025212
2169 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1191000.v1.1.491

Sequence Viewer

Length: 1689 bp
ATGCTTGCTAGGCTTCAAAGGTTTGACGAGTGCAAGAAATTCAAGAACTACAAAGGCGGTTCCATGTTAAGTCCAAACGCGCATTCGTTCGATCCCAAAAGGCCAAAGATGTTGATAGCCAAAGCAATAGAAATTGAGCAAGCTGAGAAGACCCGCAATAAGGCCGTTCTGTCTGGGCAAGAGTTGACTGAGTTACTTGGTGGACAAGAAACTGGTGCTAGTAATGGAAGCTGCCGGGGATGTACTTGCAGGGTTGGAAGCTATGGACTGAAGGCTTTCACAAGTGACTTAAGAAGCAAGTTTAAGGGTTTGGATGATATATATGTGTGGCATGCTCTTTGTGGGGCCTGGTGTGGTGTTAAGCCTGGTGCAACCCATCTCAATGCTAAGATCACTCTCTGCGTTCTCCCTCCTGGACTTGACGGAACTATGAATGATCTCGCGGTGGATAAAGTGCTTGAAGGAGGAATGGGGCTTGTTCATCCCGATTATGCTTCTCTTTTATATGACTCAATGCATTCGTACCTCTCTGAAGTTGGCGTCACAGGAGTCAAAGTGGATGTTATTCATATTCTTGAATATGTGTCTGAGGAACATGGAGGTCGCGTGGAGCTTGCGAAGGCTTATTACAAGGGGTTGAGTGATTCTCTTGCGAAGAACTTCAATGGGAGTGGACTTATTTGTAGCATTCAGCAATGCAACGACTTCTTCTTCCTCGGGACAAAGCAAATTTCCATGGGAAGAGCTGGTGATGATTTTTGGTTCCAGGACCCAAATGGAGATCCGATGGGAGTTTACTGGCTGCAAGGTGTTCATATGATCCACTGCTCCTACAACAGCATGTGGATGGGGCAGATGATCGTGCCGGATTGGGATATGTTCCAATCAGACCATCTTTGTGCCAAATATCATGCCGGGTCTAGGGCTATCTGTGGAGGTCCGGTGTATTTAAGCGACTTCGTTGGTAGTCATGATTTTGATCTCATCAAGAAGCTTGTTCATCCAGATGGACCCGTTCCCAATTGCTTGCATTATGCCCTTCCAACCAAAGACTGCCTCTTCAAAAACCCTTTGTTTGACGGCAAAACTGCTCTCAAAATTTGGAACTTCAACAAGTTTGGTGGTGTGATTGGAGGATTCAACTGCCAAGGAGCAGGTTGGGACCCCAAGGAGCAAAGGATCAAAGGCTTTCCAGATTGCTACAAGCCAATCCTTTGTTCAGTGCATGTTTTTGATATTGAATGGGACCAAAACTTAGAACTAGCTCATATGGGAAAGGCTGAAGAGTACATTGTCCATCTCAACCAGGATGATGAACTCTGCATTTTGACTCCAAAATCTGATGTTATTCAAATCACTATCCAACCTTCTTCTTTTGAGATTTTCACCATTGTGCCGGTCCAAACCCTCAGCCCGGGCAACAAATTTGCTTCGGTGGGACTTACAAACATGTTCAACAGTGGAGGAAGCATCCAGGAATTGGAGTACACAATTGAGGGGGGAGTTAGTGCAAAGGTGAAGGTCAAAGGAGGAGGCTGTTTCTTGGCCTACTCGAGTGGGTGTCCAAAGAAATGTTATCTGAATGGTGGAGAGGTGGCTTTTGAGTGGTCTGATGAGGGCAAACTGAAGCTCAATCTTCTTTGGGTTGAAGAAGCTGCTGGCATTTCTGAGTTAGTTTTTATGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0006950 GO:0006979 GO:0008150 GO:0008378 GO:0009628 GO:0016740 GO:0016757 GO:0016758 GO:0047268 GO:0050896 GO:0080167
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

563

Amino Acids

61.9

Weight (kDa)

5.87

Isoelectric Point (pI)

33.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 1 - 545 1.5e-203 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028378)

Species Orthologous Gene IDs
malus_domestica MD15G1191000.v1.1
rosa_multiflora Rmu_sc0005947.1_g000018

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1145
AccB7I CCANNNNNTGG 1 cut(s) 1480
AccII CGCG 3 cut(s) 80, 443, 606
AciI CCGC 3 cut(s) 57, 154, 443
AclWI GGATC 4 cut(s) 86, 776, 814, 1187
AcsI RAATTY 4 cut(s) 38, 729, 1098, 1424
AcuI CTGAAG 4 cut(s) 290, 552, 1302, 1646
AcyI GRCGYC 1 cut(s) 540
AfaI GTAC 4 cut(s) 244, 524, 1289, 1487
AfiI CCNNNNNNNGG 4 cut(s) 160, 921, 1414, 1480
AflII CTTAAG 1 cut(s) 289
AflIII ACRYGT 1 cut(s) 1449
AjnI CCWGG 6 cut(s) 347, 364, 412, 765, 1305, 1473
AjuI GAANNNNNNNTTGG 4 cut(s) 67, 99, 1201, 1233
AleI CACNNNNGTG 1 cut(s) 1391
AluBI AGCT 9 cut(s) 143, 231, 261, 613, 746, 994, 1265, 1630, 1655
AluI AGCT 9 cut(s) 143, 231, 261, 613, 746, 994, 1265, 1630, 1655
AlwI GGATC 4 cut(s) 86, 776, 814, 1187
Ama87I CYCGRG 3 cut(s) 716, 1414, 1552
AoxI GGCC 4 cut(s) 101, 162, 345, 1545
ApeKI GCWGC 3 cut(s) 231, 802, 1655
ApoI RAATTY 4 cut(s) 38, 729, 1098, 1424
Asp700I GAANNNNTTC 2 cut(s) 275, 659
AspLEI GCGC 1 cut(s) 82
AspS9I GGNCC 7 cut(s) 345, 769, 938, 1010, 1162, 1246, 1399
AsuC2I CCSGG 4 cut(s) 236, 916, 1415, 1416
AsuHPI GGTGA 3 cut(s) 761, 1378, 1528
AvaI CYCGRG 3 cut(s) 716, 1414, 1552
AvaII GGWCC 6 cut(s) 769, 938, 1010, 1162, 1246, 1399
BarI GAAGNNNNNNTAC 2 cut(s) 611, 643
BbsI GAAGAC 1 cut(s) 155
BbvCI CCTCAGC 1 cut(s) 1409
BbvI GCAGC 3 cut(s) 218, 789, 1642
BccI CCATC 6 cut(s) 384, 781, 841, 900, 1001, 1305
BceAI ACGGC 2 cut(s) 149, 1096
BciT130I CCWGG 6 cut(s) 349, 366, 414, 767, 1307, 1475
BcnI CCSGG 4 cut(s) 236, 916, 1415, 1416
BfaI CTAG 4 cut(s) 9, 219, 921, 1262
BfrI CTTAAG 1 cut(s) 289
BfuAI ACCTGC 1 cut(s) 1145
BisI GCNGC 3 cut(s) 232, 803, 1656
BlsI GCNGC 3 cut(s) 233, 804, 1657
Bme18I GGWCC 6 cut(s) 769, 938, 1010, 1162, 1246, 1399
BmeT110I CYCGRG 3 cut(s) 716, 1414, 1552
BmgT120I GGNCC 7 cut(s) 345, 769, 938, 1010, 1162, 1246, 1399
BmiI GGNNCC 8 cut(s) 61, 346, 764, 771, 1012, 1163, 1164, 1247
BmsI GCATC 1 cut(s) 1479
BpiI GAAGAC 1 cut(s) 155
BplI GAGNNNNNCTC 2 cut(s) 631, 663
Bpu10I CCTNAGC 1 cut(s) 1409
BpuMI CCSGG 4 cut(s) 236, 916, 1415, 1416
BsaBI GATNNNNATC 1 cut(s) 978
BsaHI GRCGYC 1 cut(s) 540
BsaJI CCNNGG 6 cut(s) 235, 715, 735, 1147, 1167, 1414
BsaWI WCCGGW 1 cut(s) 940
BsaXI ACNNNNNCTCC 6 cut(s) 540, 570, 591, 621, 927, 957
Bsc4I CCNNNNNNNGG 4 cut(s) 160, 921, 1414, 1480
Bse118I RCCGGY 1 cut(s) 1396
Bse1I ACTGG 2 cut(s) 217, 803
Bse3DI GCAATG 1 cut(s) 701
Bse8I GATNNNNATC 1 cut(s) 978
BseBI CCWGG 6 cut(s) 349, 366, 414, 767, 1307, 1475
BseDI CCNNGG 6 cut(s) 235, 715, 735, 1147, 1167, 1414
BseGI GGATG 8 cut(s) 245, 319, 481, 565, 852, 1000, 1315, 1470
BseJI GATNNNNATC 1 cut(s) 978
BseLI CCNNNNNNNGG 4 cut(s) 160, 921, 1414, 1480
BseMI GCAATG 1 cut(s) 701
BseMII CTCAG 5 cut(s) 135, 180, 579, 1423, 1659
BseNI ACTGG 2 cut(s) 217, 803
BseRI GAGGAG 1 cut(s) 1545
BseXI GCAGC 3 cut(s) 218, 789, 1642
Bsh1236I CGCG 3 cut(s) 80, 443, 606
BshFI GGCC 4 cut(s) 103, 164, 347, 1547
BsiHKCI CYCGRG 3 cut(s) 716, 1414, 1552
BsiSI CCGG 6 cut(s) 235, 866, 915, 941, 1397, 1415
BslFI GGGAC 4 cut(s) 733, 1175, 1259, 1452
BslI CCNNNNNNNGG 4 cut(s) 160, 921, 1414, 1480
BsmFI GGGAC 4 cut(s) 733, 1175, 1259, 1452
BsmI GAATGC 3 cut(s) 82, 517, 687
BsnI GGCC 4 cut(s) 103, 164, 347, 1547
BsoBI CYCGRG 3 cut(s) 716, 1414, 1552
Bsp143I GATC 8 cut(s) 91, 390, 436, 781, 819, 858, 979, 1179
Bsp19I CCATGG 1 cut(s) 735
BspACI CCGC 3 cut(s) 57, 154, 443
BspANI GGCC 4 cut(s) 103, 164, 347, 1547
BspCNI CTCAG 5 cut(s) 136, 181, 580, 1422, 1660
BspFNI CGCG 3 cut(s) 80, 443, 606
BspHI TCATGA 1 cut(s) 970
BspLI GGNNCC 8 cut(s) 61, 346, 764, 771, 1012, 1163, 1164, 1247
BspMI ACCTGC 1 cut(s) 1145
BspPI GGATC 4 cut(s) 86, 776, 814, 1187
BspQI GCTCTTC 1 cut(s) 736
BspTI CTTAAG 1 cut(s) 289
BsrDI GCAATG 1 cut(s) 701
BsrFI RCCGGY 1 cut(s) 1396
BsrI ACTGG 2 cut(s) 217, 803
BssAI RCCGGY 1 cut(s) 1396
BssECI CCNNGG 6 cut(s) 235, 715, 735, 1147, 1167, 1414
BssMI GATC 8 cut(s) 91, 390, 436, 781, 819, 858, 979, 1179
BssNI GRCGYC 1 cut(s) 540
BssT1I CCWWGG 3 cut(s) 735, 1147, 1167
Bst2UI CCWGG 6 cut(s) 349, 366, 414, 767, 1307, 1475
Bst4CI ACNGT 1 cut(s) 1460
Bst6I CTCTTC 3 cut(s) 736, 1064, 1278
BstACI GRCGYC 1 cut(s) 540
BstAFI CTTAAG 1 cut(s) 289
BstC8I GCNNGC 6 cut(s) 6, 141, 333, 615, 1028, 1660
BstDEI CTNAG 7 cut(s) 144, 189, 387, 588, 1255, 1409, 1668
BstDSI CCRYGG 1 cut(s) 735
BstF5I GGATG 8 cut(s) 245, 319, 481, 565, 852, 1000, 1315, 1470
BstFNI CGCG 3 cut(s) 80, 443, 606
BstHHI GCGC 1 cut(s) 82
BstKTI GATC 8 cut(s) 94, 393, 439, 784, 822, 861, 982, 1182
BstMBI GATC 8 cut(s) 91, 390, 436, 781, 819, 858, 979, 1179
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstNI CCWGG 6 cut(s) 349, 366, 414, 767, 1307, 1475
BstNSI RCATGY 4 cut(s) 335, 844, 1229, 1453
BstUI CGCG 3 cut(s) 80, 443, 606
BstV1I GCAGC 3 cut(s) 218, 789, 1642
BstV2I GAAGAC 1 cut(s) 155
BstX2I RGATCY 1 cut(s) 781
BstYI RGATCY 1 cut(s) 781
BsuRI GGCC 4 cut(s) 103, 164, 347, 1547
BtgI CCRYGG 1 cut(s) 735
BtsCI GGATG 8 cut(s) 245, 319, 481, 565, 852, 1000, 1315, 1470
BtsI GCAGTG 1 cut(s) 823
BtsIMutI CAGTG 3 cut(s) 823, 1227, 1465
BveI ACCTGC 1 cut(s) 1145
Cac8I GCNNGC 6 cut(s) 6, 141, 333, 615, 1028, 1660
CciI TCATGA 1 cut(s) 970
CfoI GCGC 1 cut(s) 82
Cfr10I RCCGGY 1 cut(s) 1396
Cfr13I GGNCC 7 cut(s) 345, 769, 938, 1010, 1162, 1246, 1399
Cfr9I CCCGGG 1 cut(s) 1414
CseI GACGC 1 cut(s) 529
Csp6I GTAC 4 cut(s) 243, 523, 1288, 1486
CspCI CAANNNNNGTGG 4 cut(s) 652, 687, 1102, 1137
CviAII CATG 9 cut(s) 64, 332, 596, 736, 841, 911, 971, 1226, 1450
CviQI GTAC 4 cut(s) 243, 523, 1288, 1486
DdeI CTNAG 7 cut(s) 144, 189, 387, 588, 1255, 1409, 1668
DpnI GATC 8 cut(s) 93, 392, 438, 783, 821, 860, 981, 1181
DpnII GATC 8 cut(s) 91, 390, 436, 781, 819, 858, 979, 1179
Eam1104I CTCTTC 3 cut(s) 736, 1064, 1278
EarI CTCTTC 3 cut(s) 736, 1064, 1278
Eco130I CCWWGG 3 cut(s) 735, 1147, 1167
Eco47I GGWCC 6 cut(s) 769, 938, 1010, 1162, 1246, 1399
Eco57I CTGAAG 4 cut(s) 290, 552, 1302, 1646
Eco88I CYCGRG 3 cut(s) 716, 1414, 1552
EcoO109I RGGNCCY 3 cut(s) 345, 769, 1162
EcoRII CCWGG 6 cut(s) 347, 364, 412, 765, 1305, 1473
EcoT14I CCWWGG 3 cut(s) 735, 1147, 1167
EcoT22I ATGCAT 1 cut(s) 519
ErhI CCWWGG 3 cut(s) 735, 1147, 1167
FaeI CATG 9 cut(s) 67, 335, 599, 739, 844, 914, 974, 1229, 1453
FaqI GGGAC 4 cut(s) 733, 1175, 1259, 1452
FatI CATG 9 cut(s) 63, 331, 595, 735, 840, 910, 970, 1225, 1449
FauI CCCGC 1 cut(s) 161
FauNDI CATATG 2 cut(s) 816, 1269
Fnu4HI GCNGC 3 cut(s) 232, 803, 1656
FokI GGATG 8 cut(s) 252, 326, 468, 572, 859, 987, 1322, 1457
Fsp4HI GCNGC 3 cut(s) 232, 803, 1656
FspBI CTAG 4 cut(s) 9, 219, 921, 1262
GlaI GCGC 1 cut(s) 81
GluI GCNGC 3 cut(s) 232, 803, 1656
HaeIII GGCC 4 cut(s) 103, 164, 347, 1547
HapII CCGG 6 cut(s) 235, 866, 915, 941, 1397, 1415
HgaI GACGC 1 cut(s) 529
HhaI GCGC 1 cut(s) 82
Hin1I GRCGYC 1 cut(s) 540
Hin1II CATG 9 cut(s) 67, 335, 599, 739, 844, 914, 974, 1229, 1453
Hin6I GCGC 1 cut(s) 80
HinP1I GCGC 1 cut(s) 80
HincII GTYRAC 1 cut(s) 186
HindII GTYRAC 1 cut(s) 186
HindIII AAGCTT 1 cut(s) 992
HinfI GANTC 5 cut(s) 509, 549, 644, 1137, 1330
HpaII CCGG 6 cut(s) 235, 866, 915, 941, 1397, 1415
HphI GGTGA 3 cut(s) 761, 1378, 1528
Hpy166II GTNNAC 5 cut(s) 186, 203, 674, 796, 1488
Hpy188I TCNGA 8 cut(s) 532, 589, 786, 889, 1342, 1581, 1612, 1669
Hpy188III TCNNGA 8 cut(s) 43, 485, 575, 718, 971, 988, 1004, 1193
Hpy8I GTNNAC 5 cut(s) 186, 203, 674, 796, 1488
HpyAV CCTTC 6 cut(s) 265, 455, 613, 1049, 1377, 1513
HpyCH4III ACNGT 1 cut(s) 1460
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
HpyF3I CTNAG 7 cut(s) 144, 189, 387, 588, 1255, 1409, 1668
Hsp92I GRCGYC 1 cut(s) 540
Hsp92II CATG 9 cut(s) 67, 335, 599, 739, 844, 914, 974, 1229, 1453
HspAI GCGC 1 cut(s) 80
KflI GGGWCCC 1 cut(s) 1162
Kzo9I GATC 8 cut(s) 91, 390, 436, 781, 819, 858, 979, 1179
LguI GCTCTTC 1 cut(s) 736
LmnI GCTCC 4 cut(s) 610, 833, 1151, 1171
Lsp1109I GCAGC 3 cut(s) 218, 789, 1642
LweI GCATC 1 cut(s) 1479
MaeI CTAG 4 cut(s) 9, 219, 921, 1262
MaeIII GTNAC 3 cut(s) 192, 284, 541
MalI GATC 8 cut(s) 93, 392, 438, 783, 821, 860, 981, 1181
MboI GATC 8 cut(s) 91, 390, 436, 781, 819, 858, 979, 1179
MfeI CAATTG 2 cut(s) 1021, 1491
MflI RGATCY 1 cut(s) 781
MluCI AATT 8 cut(s) 38, 132, 729, 1021, 1098, 1424, 1478, 1491
MlyI GAGTC 3 cut(s) 503, 558, 1324
MmeI TCCRAC 3 cut(s) 235, 1067, 1387
Mph1103I ATGCAT 1 cut(s) 519
MroXI GAANNNNTTC 2 cut(s) 275, 659
MseI TTAA 5 cut(s) 68, 290, 303, 360, 950
MslI CAYNNNNRTG 5 cut(s) 381, 845, 897, 1005, 1391
MspCI CTTAAG 1 cut(s) 289
MspI CCGG 6 cut(s) 235, 866, 915, 941, 1397, 1415
MunI CAATTG 2 cut(s) 1021, 1491
Mva1269I GAATGC 3 cut(s) 82, 517, 687
MvaI CCWGG 6 cut(s) 349, 366, 414, 767, 1307, 1475
MvnI CGCG 3 cut(s) 80, 443, 606
MwoI GCNNNNNNNGC 1 cut(s) 10
NciI CCSGG 4 cut(s) 236, 916, 1415, 1416
NcoI CCATGG 1 cut(s) 735
NdeI CATATG 2 cut(s) 816, 1269
NdeII GATC 8 cut(s) 91, 390, 436, 781, 819, 858, 979, 1179
NlaIII CATG 9 cut(s) 67, 335, 599, 739, 844, 914, 974, 1229, 1453
NlaIV GGNNCC 8 cut(s) 61, 346, 764, 771, 1012, 1163, 1164, 1247
NmuCI GTSAC 2 cut(s) 284, 541
NsiI ATGCAT 1 cut(s) 519
NspI RCATGY 4 cut(s) 335, 844, 1229, 1453
OliI CACNNNNGTG 1 cut(s) 1391
PaeI GCATGC 1 cut(s) 335
PaeR7I CTCGAG 1 cut(s) 1552
PagI TCATGA 1 cut(s) 970
PciI ACATGT 1 cut(s) 1449
PciSI GCTCTTC 1 cut(s) 736
PctI GAATGC 3 cut(s) 82, 517, 687
PdmI GAANNNNTTC 2 cut(s) 275, 659
PfeI GAWTC 2 cut(s) 644, 1137
PflMI CCANNNNNTGG 1 cut(s) 1480
PfoI TCCNGGA 3 cut(s) 412, 765, 1473
PkrI GCNGC 3 cut(s) 233, 804, 1657
PleI GAGTC 3 cut(s) 503, 557, 1324
PpsI GAGTC 3 cut(s) 503, 557, 1324
PpuMI RGGWCCY 2 cut(s) 769, 1162
PscI ACATGT 1 cut(s) 1449
Psp5II RGGWCCY 2 cut(s) 769, 1162
Psp6I CCWGG 6 cut(s) 347, 364, 412, 765, 1305, 1473
PspGI CCWGG 6 cut(s) 347, 364, 412, 765, 1305, 1473
PspN4I GGNNCC 8 cut(s) 61, 346, 764, 771, 1012, 1163, 1164, 1247
PspPI GGNCC 7 cut(s) 345, 769, 938, 1010, 1162, 1246, 1399
PspPPI RGGWCCY 2 cut(s) 769, 1162
PspXI VCTCGAGB 1 cut(s) 1552
PsuI RGATCY 1 cut(s) 781
RsaI GTAC 4 cut(s) 244, 524, 1289, 1487
RsaNI GTAC 4 cut(s) 243, 523, 1288, 1486
RseI CAYNNNNRTG 5 cut(s) 381, 845, 897, 1005, 1391
SapI GCTCTTC 1 cut(s) 736
SaqAI TTAA 5 cut(s) 68, 290, 303, 360, 950
SatI GCNGC 3 cut(s) 232, 803, 1656
Sau3AI GATC 8 cut(s) 91, 390, 436, 781, 819, 858, 979, 1179
Sau96I GGNCC 7 cut(s) 345, 769, 938, 1010, 1162, 1246, 1399
SchI GAGTC 3 cut(s) 503, 558, 1324
SfaNI GCATC 1 cut(s) 1479
Sfr274I CTCGAG 1 cut(s) 1552
SinI GGWCC 6 cut(s) 769, 938, 1010, 1162, 1246, 1399
SlaI CTCGAG 1 cut(s) 1552
SmaI CCCGGG 1 cut(s) 1416
SmiMI CAYNNNNRTG 5 cut(s) 381, 845, 897, 1005, 1391
SmlI CTYRAG 2 cut(s) 289, 1552
SmoI CTYRAG 2 cut(s) 289, 1552
SphI GCATGC 1 cut(s) 335
SrfI GCCCGGGC 1 cut(s) 1416
Sse9I AATT 8 cut(s) 38, 132, 729, 1021, 1098, 1424, 1478, 1491
SsiI CCGC 3 cut(s) 57, 154, 443
SspMI CTAG 4 cut(s) 9, 219, 921, 1262
StyI CCWWGG 3 cut(s) 735, 1147, 1167
TaaI ACNGT 1 cut(s) 1460
TaqI TCGA 2 cut(s) 90, 1553
TasI AATT 8 cut(s) 38, 132, 729, 1021, 1098, 1424, 1478, 1491
TatI WGTACW 3 cut(s) 242, 1287, 1485
TfiI GAWTC 2 cut(s) 644, 1137
Tru1I TTAA 5 cut(s) 68, 290, 303, 360, 950
Tru9I TTAA 5 cut(s) 68, 290, 303, 360, 950
TscAI CASTG 3 cut(s) 830, 1227, 1465
TseFI GTSAC 2 cut(s) 284, 541
TseI GCWGC 3 cut(s) 231, 802, 1655
Tsp45I GTSAC 2 cut(s) 284, 541
TspDTI ATGAA 6 cut(s) 446, 470, 557, 803, 989, 1329
TspGWI ACGGA 1 cut(s) 438
TspMI CCCGGG 1 cut(s) 1414
TspRI CASTG 3 cut(s) 830, 1227, 1465
Van91I CCANNNNNTGG 1 cut(s) 1480
Vha464I CTTAAG 1 cut(s) 289
VpaK11BI GGWCC 6 cut(s) 769, 938, 1010, 1162, 1246, 1399
XapI RAATTY 4 cut(s) 38, 729, 1098, 1424
XceI RCATGY 4 cut(s) 335, 844, 1229, 1453
XcmI CCANNNNNNNNNTGG 1 cut(s) 773
XhoI CTCGAG 1 cut(s) 1552
XmaI CCCGGG 1 cut(s) 1414
XmnI GAANNNNTTC 2 cut(s) 275, 659
XspI CTAG 4 cut(s) 9, 219, 921, 1262
Zsp2I ATGCAT 1 cut(s) 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.