Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0024295

Physical Location & Seq

Genomic Coordinates Reverse (-)

17961429 .. 17963993

Gene Structure 2565 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1221600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 777 bp

ATGTGTACAAGGCCGACTATTGTAGCCAAATGTTGTCGTTGTACAGTTATTGTGCTGTTCTTTTCAACATGTGATGCAGTGGATTTTCACTACTCCTTGTTAAATGAGTTCCAATTCAAACCTTATTCAGGACCAGGTGCAGAAATTAAGCAGAGGCTTGTTAATTGTAAGACCTTCCAGTTACATGGGAATATGGAGCAGGAGGATCGAAGATCTGCTTTTGAGACATTCAGAAGAGAAAAATCGGCTCTTCTTTTGTCCACAGATGTTTGTGCTAGAGGTTTGGATTTCCCGAAAGTAAAATGCATTATACAATATGATTCTCCAGGCGAGGCTACTGAATATGCTCATAGGGTTGGAAGAACTGCTCGGCTTGGTGAAAGAGGAGATTCATTGTTGTTTTTACAGCCAATTGAAATGGATTATTTGAAAGAGTTGGAGAAACATGTTGTTTCTCTAGCAGAGTTTCCCTTCCTCAAAATATTAGATGGTTATCCACTATACGGTCATAAGCATGTCAAGAAGTTTCCGGATATGAAGACATTAGCTAAGAACGCATTTTGCTCGTGGGTTCGCGCATACATTGCCCACCGCGGTGAACTGAAAAGAATTTTTATGGTAAAGAAACTTCACTTGGGACACGTTGTGAAAAGCTTTGCATTGAAAGACCAGCCTTCCTTGGTTGGTAAGTTATTCCACAATCAAGAAAAGAAAAGGATGAGCGGTGAAAAGCAAAAGGGATTATCAAGAAAGAGGAAGGTTTTGAGGAAAACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD DUF4217 Helicase_C

Protein Analysis

259

Amino Acids

29.81

Weight (kDa)

9.67

Isoelectric Point (pI)

38.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Helicase_C	PF00271	51 - 126	6.8e-22	Helicase conserved C-terminal domain
CTE_SPB4	PF13959	177 - 226	6.7e-17	ATP-dependent rRNA helicase SPB4-like, C-terminal extension

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0024295)

Species	Orthologous Gene IDs
malus_domestica	MD15G1221600.v1.1
rosa_multiflora	Rmu_sc0000298.1_g000001 Rmu_sc0036117.1_g000001

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	723
AccII	CGCG	2 cut(s)	576, 594
AccIII	TCCGGA	1 cut(s)	529
AciI	CCGC	3 cut(s)	592, 594, 723
AclWI	GGATC	1 cut(s)	213
AcsI	RAATTY	1 cut(s)	609
AdeI	CACNNNGTG	1 cut(s)	646
AfaI	GTAC	2 cut(s)	7, 43
AfiI	CCNNNNNNNGG	2 cut(s)	128, 503
AflIII	ACRYGT	3 cut(s)	68, 445, 640
AgsI	TTSAA	5 cut(s)	66, 118, 416, 430, 664
AjnI	CCWGG	2 cut(s)	133, 325
AjuI	GAANNNNNNNTTGG	2 cut(s)	617, 649
AleI	CACNNNNGTG	1 cut(s)	594
AluBI	AGCT	2 cut(s)	548, 654
AluI	AGCT	2 cut(s)	548, 654
Alw26I	GTCTC	1 cut(s)	218
AlwI	GGATC	1 cut(s)	213
Aor13HI	TCCGGA	1 cut(s)	529
AoxI	GGCC	1 cut(s)	11
ApoI	RAATTY	1 cut(s)	609
AspLEI	GCGC	1 cut(s)	578
AspS9I	GGNCC	1 cut(s)	131
AsuHPI	GGTGA	3 cut(s)	389, 608, 737
AvaII	GGWCC	1 cut(s)	131
BarI	GAAGNNNNNNTAC	2 cut(s)	612, 644
BauI	CACGAG	1 cut(s)	565
BbsI	GAAGAC	1 cut(s)	545
BccI	CCATC	1 cut(s)	482
BcgI	CGANNNNNNTGC	4 cut(s)	188, 222, 546, 580
BciT130I	CCWGG	2 cut(s)	135, 327
BcoDI	GTCTC	1 cut(s)	218
BfaI	CTAG	2 cut(s)	276, 458
BglII	AGATCT	1 cut(s)	212
Bme1390I	CCNGG	2 cut(s)	135, 327
Bme18I	GGWCC	1 cut(s)	131
BmgT120I	GGNCC	1 cut(s)	131
BmrFI	CCNGG	2 cut(s)	135, 327
BmsI	GCATC	1 cut(s)	64
BpiI	GAAGAC	1 cut(s)	545
BpmI	CTGGAG	1 cut(s)	309
BsaBI	GATNNNNATC	1 cut(s)	492
BsaJI	CCNNGG	2 cut(s)	592, 678
BsaWI	WCCGGW	1 cut(s)	529
Bsc4I	CCNNNNNNNGG	2 cut(s)	128, 503
Bse1I	ACTGG	1 cut(s)	178
Bse3DI	GCAATG	1 cut(s)	582
Bse8I	GATNNNNATC	1 cut(s)	492
BseAI	TCCGGA	1 cut(s)	529
BseBI	CCWGG	2 cut(s)	135, 327
BseDI	CCNNGG	2 cut(s)	592, 678
BseGI	GGATG	1 cut(s)	723
BseJI	GATNNNNATC	1 cut(s)	492
BseLI	CCNNNNNNNGG	2 cut(s)	128, 503
BseMI	GCAATG	1 cut(s)	582
BseNI	ACTGG	1 cut(s)	178
BseRI	GAGGAG	1 cut(s)	399
BsgI	GTGCAG	1 cut(s)	159
Bsh1236I	CGCG	2 cut(s)	576, 594
BshFI	GGCC	1 cut(s)	13
BsiSI	CCGG	1 cut(s)	530
BslFI	GGGAC	1 cut(s)	651
BslI	CCNNNNNNNGG	2 cut(s)	128, 503
BsmAI	GTCTC	1 cut(s)	218
BsmFI	GGGAC	1 cut(s)	651
BsnI	GGCC	1 cut(s)	13
Bsp13I	TCCGGA	1 cut(s)	529
Bsp1407I	TGTACA	2 cut(s)	5, 41
Bsp143I	GATC	2 cut(s)	205, 212
BspACI	CCGC	3 cut(s)	592, 594, 723
BspANI	GGCC	1 cut(s)	13
BspEI	TCCGGA	1 cut(s)	529
BspFNI	CGCG	2 cut(s)	576, 594
BspPI	GGATC	1 cut(s)	213
BspQI	GCTCTTC	1 cut(s)	255
BsrBI	CCGCTC	1 cut(s)	723
BsrDI	GCAATG	1 cut(s)	582
BsrGI	TGTACA	2 cut(s)	5, 41
BsrI	ACTGG	1 cut(s)	178
BssECI	CCNNGG	2 cut(s)	592, 678
BssMI	GATC	2 cut(s)	205, 212
BssSI	CACGAG	1 cut(s)	565
BssT1I	CCWWGG	1 cut(s)	678
Bst2BI	CACGAG	1 cut(s)	565
Bst2UI	CCWGG	2 cut(s)	135, 327
Bst4CI	ACNGT	2 cut(s)	46, 506
Bst6I	CTCTTC	2 cut(s)	229, 255
BstAPI	GCANNNNNTGC	1 cut(s)	584
BstAUI	TGTACA	2 cut(s)	5, 41
BstDEI	CTNAG	1 cut(s)	549
BstDSI	CCRYGG	1 cut(s)	592
BstENI	CCTNNNNNAGG	1 cut(s)	126
BstF5I	GGATG	1 cut(s)	723
BstFNI	CGCG	2 cut(s)	576, 594
BstHHI	GCGC	1 cut(s)	578
BstKTI	GATC	2 cut(s)	208, 215
BstMAI	GTCTC	1 cut(s)	218
BstMBI	GATC	2 cut(s)	205, 212
BstMWI	GCNNNNNNNGC	2 cut(s)	554, 584
BstNI	CCWGG	2 cut(s)	135, 327
BstNSI	RCATGY	3 cut(s)	72, 449, 518
BstSCI	CCNGG	2 cut(s)	133, 325
BstUI	CGCG	2 cut(s)	576, 594
BstV2I	GAAGAC	1 cut(s)	545
BstX2I	RGATCY	1 cut(s)	212
BstXI	CCANNNNNNTGG	1 cut(s)	185
BstYI	RGATCY	1 cut(s)	212
BsuRI	GGCC	1 cut(s)	13
BtgI	CCRYGG	1 cut(s)	592
BtsCI	GGATG	1 cut(s)	723
BtsI	GCAGTG	1 cut(s)	84
BtsIMutI	CAGTG	1 cut(s)	84
CfoI	GCGC	1 cut(s)	578
Cfr13I	GGNCC	1 cut(s)	131
Cfr42I	CCGCGG	1 cut(s)	595
CsiI	ACCWGGT	1 cut(s)	133
Csp6I	GTAC	2 cut(s)	6, 42
CviAII	CATG	5 cut(s)	69, 185, 446, 515, 774
CviQI	GTAC	2 cut(s)	6, 42
DdeI	CTNAG	1 cut(s)	549
DpnI	GATC	2 cut(s)	207, 214
DpnII	GATC	2 cut(s)	205, 212
DraIII	CACNNNGTG	1 cut(s)	646
Eam1104I	CTCTTC	2 cut(s)	229, 255
EarI	CTCTTC	2 cut(s)	229, 255
Eco130I	CCWWGG	1 cut(s)	678
Eco47I	GGWCC	1 cut(s)	131
EcoNI	CCTNNNNNAGG	1 cut(s)	126
EcoRII	CCWGG	2 cut(s)	133, 325
EcoT14I	CCWWGG	1 cut(s)	678
EcoT22I	ATGCAT	1 cut(s)	308
ErhI	CCWWGG	1 cut(s)	678
FaeI	CATG	5 cut(s)	72, 188, 449, 518, 777
FalI	AAGNNNNNCTT	2 cut(s)	202, 234
FaqI	GGGAC	1 cut(s)	651
FatI	CATG	5 cut(s)	68, 184, 445, 514, 773
FokI	GGATG	1 cut(s)	730
FspBI	CTAG	2 cut(s)	276, 458
GlaI	GCGC	1 cut(s)	577
GsuI	CTGGAG	1 cut(s)	309
HaeIII	GGCC	1 cut(s)	13
HapII	CCGG	1 cut(s)	530
HhaI	GCGC	1 cut(s)	578
Hin1II	CATG	5 cut(s)	72, 188, 449, 518, 777
Hin6I	GCGC	1 cut(s)	576
HinP1I	GCGC	1 cut(s)	576
HindIII	AAGCTT	1 cut(s)	652
HinfI	GANTC	2 cut(s)	320, 389
HpaII	CCGG	1 cut(s)	530
HphI	GGTGA	3 cut(s)	389, 608, 737
Hpy166II	GTNNAC	3 cut(s)	6, 261, 599
Hpy188I	TCNGA	1 cut(s)	233
Hpy188III	TCNNGA	6 cut(s)	129, 292, 520, 530, 704, 747
Hpy8I	GTNNAC	3 cut(s)	6, 261, 599
HpyAV	CCTTC	4 cut(s)	184, 481, 684, 751
HpyCH4III	ACNGT	2 cut(s)	46, 506
HpyCH4IV	ACGT	1 cut(s)	642
HpyCH4V	TGCA	4 cut(s)	77, 140, 306, 659
HpyF10VI	GCNNNNNNNGC	2 cut(s)	554, 584
HpyF3I	CTNAG	1 cut(s)	549
HpySE526I	ACGT	1 cut(s)	642
Hsp92II	CATG	5 cut(s)	72, 188, 449, 518, 777
HspAI	GCGC	1 cut(s)	576
Kpn2I	TCCGGA	1 cut(s)	529
KspI	CCGCGG	1 cut(s)	595
Kzo9I	GATC	2 cut(s)	205, 212
LguI	GCTCTTC	1 cut(s)	255
LmnI	GCTCC	1 cut(s)	196
LpnPI	CCDG	9 cut(s)	114, 120, 147, 185, 191, 312, 339, 543, 683
LweI	GCATC	1 cut(s)	64
MabI	ACCWGGT	1 cut(s)	133
MaeI	CTAG	2 cut(s)	276, 458
MaeII	ACGT	1 cut(s)	642
MaeIII	GTNAC	1 cut(s)	180
MalI	GATC	2 cut(s)	207, 214
MbiI	CCGCTC	1 cut(s)	723
MboI	GATC	2 cut(s)	205, 212
MboII	GAAGA	5 cut(s)	222, 242, 246, 372, 550
MfeI	CAATTG	1 cut(s)	411
MflI	RGATCY	1 cut(s)	212
MluCI	AATT	5 cut(s)	113, 144, 163, 411, 609
MmeI	TCCRAC	2 cut(s)	337, 417
MnlI	CCTC	8 cut(s)	147, 196, 272, 325, 377, 485, 747, 759
Mph1103I	ATGCAT	1 cut(s)	308
MroI	TCCGGA	1 cut(s)	529
MseI	TTAA	3 cut(s)	101, 147, 162
MslI	CAYNNNNRTG	2 cut(s)	513, 594
MspA1I	CMGCKG	1 cut(s)	594
MspI	CCGG	1 cut(s)	530
MspR9I	CCNGG	2 cut(s)	135, 327
MunI	CAATTG	1 cut(s)	411
MvaI	CCWGG	2 cut(s)	135, 327
MvnI	CGCG	2 cut(s)	576, 594
MwoI	GCNNNNNNNGC	2 cut(s)	554, 584
NdeII	GATC	2 cut(s)	205, 212
NlaIII	CATG	5 cut(s)	72, 188, 449, 518, 777
NmeAIII	GCCGAG	1 cut(s)	349
NsiI	ATGCAT	1 cut(s)	308
NspI	RCATGY	3 cut(s)	72, 449, 518
OliI	CACNNNNGTG	1 cut(s)	594
PciI	ACATGT	2 cut(s)	68, 445
PciSI	GCTCTTC	1 cut(s)	255
PfeI	GAWTC	2 cut(s)	320, 389
PscI	ACATGT	2 cut(s)	68, 445
Psp6I	CCWGG	2 cut(s)	133, 325
PspGI	CCWGG	2 cut(s)	133, 325
PspPI	GGNCC	1 cut(s)	131
PsuI	RGATCY	1 cut(s)	212
RsaI	GTAC	2 cut(s)	7, 43
RsaNI	GTAC	2 cut(s)	6, 42
RseI	CAYNNNNRTG	2 cut(s)	513, 594
SacII	CCGCGG	1 cut(s)	595
SapI	GCTCTTC	1 cut(s)	255
SaqAI	TTAA	3 cut(s)	101, 147, 162
Sau3AI	GATC	2 cut(s)	205, 212
Sau96I	GGNCC	1 cut(s)	131
ScrFI	CCNGG	2 cut(s)	135, 327
SetI	ASST	8 cut(s)	124, 139, 176, 283, 550, 645, 656, 762
SexAI	ACCWGGT	1 cut(s)	133
SfaNI	GCATC	1 cut(s)	64
Sfr303I	CCGCGG	1 cut(s)	595
SgrBI	CCGCGG	1 cut(s)	595
SinI	GGWCC	1 cut(s)	131
SmiMI	CAYNNNNRTG	2 cut(s)	513, 594
Sse9I	AATT	5 cut(s)	113, 144, 163, 411, 609
SsiI	CCGC	3 cut(s)	592, 594, 723
SspI	AATATT	1 cut(s)	483
SspMI	CTAG	2 cut(s)	276, 458
StyD4I	CCNGG	2 cut(s)	133, 325
StyI	CCWWGG	1 cut(s)	678
TaaI	ACNGT	2 cut(s)	46, 506
TaiI	ACGT	1 cut(s)	645
TaqI	TCGA	1 cut(s)	208
TasI	AATT	5 cut(s)	113, 144, 163, 411, 609
TatI	WGTACW	2 cut(s)	5, 41
TfiI	GAWTC	2 cut(s)	320, 389
Tru1I	TTAA	3 cut(s)	101, 147, 162
Tru9I	TTAA	3 cut(s)	101, 147, 162
TscAI	CASTG	1 cut(s)	84
TspDTI	ATGAA	2 cut(s)	381, 551
TspRI	CASTG	1 cut(s)	84
VpaK11BI	GGWCC	1 cut(s)	131
XagI	CCTNNNNNAGG	1 cut(s)	126
XapI	RAATTY	1 cut(s)	609
XceI	RCATGY	3 cut(s)	72, 449, 518
XspI	CTAG	2 cut(s)	276, 458
Zsp2I	ATGCAT	1 cut(s)	308

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.