MD15G1224500.v1.1

Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Unwinds duplex DNA. Component of the meiotic recombination pathway. Seems to play a role in mediating chromosome homology search, chromosome pairing and synapsis at early stages and probably chromosome crossing-over at later stages in meiosis. Probably is involved in the repair of meiotic double strand breaks (DBSs) and in homologous recombination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014309
Physical Location & Seq
Reverse (-)
18256026 .. 18258794
2769 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1224500.v1.1.491

Sequence Viewer

Length: 1023 bp
ATGGAGCAGCAGAGCAATCAGAAGACTGCCCAGCAACAAGACGAGCTCCAAGAAATGCACCATGGCCCTGTCCCCGTCGATCAACTTCAGGCGTCCGGCATAGCTTCCATTGATGTAAAGAAACTTAAAGATGCGGGTCTTTGCACTGTTGAAGCTGTCGCTTACTCTCCCAGGAAGGATCTTCTGCAAATCAAAGGAATCAGCGACGCTAAAGTTGACAAGATCATCGAAGCAGCCTCCAAACTTGTGCCTTTGGGTTTTACTAGCGCTGGCCAGCTCCACGCACAGAGACTTGAAATCATTCAAATTACTTCTGGATCAAGAGAACTTGATAAGATATTGGAGGGAGGAATTGAGACAGGATCTATTACCGAGATATATGGCGAATTCCGTTCTGGAAAGACCCAGTTGTGTCACACGCTTTGTGTAACTTGCCAACTTCCCTTGGATCAAGGAGGCGGTGAAGGAAAAGCAATGTACATAGATGCTGAGGGTACATTCAGGCCACAAAGACTCTTACAGATTGCAGAGAGGTTTGGACTCAATGGTGCTGATGTCTTGGAGAATGTGGCGTATGCTAGAGCTTATAACACGGATCACCAATCAAGGCTCTTGCTCGAAGCAGCCTCCATGATGGTAGAAACAAGGTTTGCTCTCATGATAGTAGATAGTGCTACAGCCCTGTACAGAACAGATTTCTCAGGAAGAGGTGAACTTTCAGCCCGTCAAATGCATCTTGCAAAGTTTCTCCGGAGCCTTCAGAAGTTAGCAGATGAGTTTGGCGTAGCTGTTGTGATCACAAATCAAGTGGTTGCCCAAGTGGATGGTTCTGCAATCTTTGCCGGTCCTCAAATTAAGCCTATTGGTGGTAACATCATGGCTCATGCTTCCACCACAAGGCTTGCTGTTAGGAAAGGAAGAGGCGAAGAGCGCATCTGTAAAGTGATAAGTTCTCCTTGTTTGGCCGAAGCAGAAGCACGGTTTCAGATCTCCCCGGAAGGTGTCACCGACGTCAAGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000150 GO:0000217 GO:0000228 GO:0000280 GO:0000400 GO:0000724 GO:0000725 GO:0000730 GO:0000793 GO:0000794 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006312 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0008094 GO:0008150 GO:0008152 GO:0009314 GO:0009628 GO:0009889 GO:0009987 GO:0010212 GO:0010332 GO:0010468 GO:0010556 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0022402 GO:0022414 GO:0022607 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0033554 GO:0034622 GO:0034641 GO:0035825 GO:0042148 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045003 GO:0046483 GO:0048285 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051276 GO:0051321 GO:0051716 GO:0060255 GO:0061982 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070192 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090735 GO:0097159 GO:0140013 GO:0140097 GO:1901360 GO:1901363 GO:1903046 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03440 ko03460 ko05200 ko05212 map03440 map03460 map05200 map05212
Pfam Domains
Protein Families
HHH_5 Rad51

Protein Analysis

341

Amino Acids

36.87

Weight (kDa)

5.88

Isoelectric Point (pI)

36.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HHH_5 PF14520 33 - 80 1.6e-06 Helix-hairpin-helix domain
RecA_N PF00154 93 - 306 6.2e-12 RecA N-terminal domain
AAA_25 PF13481 94 - 268 4.3e-09 AAA domain
Rad51 PF08423 96 - 338 3.1e-130 Rad51
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014309)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20850 AT5G20850
fragaria_vesca FvH4_1g09550 FvH4_1g09550
malus_domestica MD15G1224500.v1.1
prunus_persica Prupe.7G190800_v2.0.a1 Prupe.7G190800_v2.0.a1
pyrus_communis pycom15g19970
rosa_chinensis RchiOBHm_Chr2g0096301
rosa_laevigata RLG00000016633
rosa_multiflora Rmu_sc0005996.1_g000022
rosa_roxburghii Rroxscaffold_2G00145680
rosa_rugosa Rorug02G0057600
rosa_samantha Rh2AG104400 Rh2BG106500 Rh2CG108400
rosa_wichuraiana Rw2G008040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 588
AatII GACGTC 1 cut(s) 1014
AccIII TCCGGA 1 cut(s) 750
AciI CCGC 2 cut(s) 134, 459
AclWI GGATC 5 cut(s) 186, 325, 370, 456, 603
AcoI YGGCCR 2 cut(s) 271, 963
AcsI RAATTY 1 cut(s) 386
AcuI CTGAAG 2 cut(s) 71, 743
AcyI GRCGYC 2 cut(s) 92, 1011
AfaI GTAC 3 cut(s) 479, 496, 686
AfeI AGCGCT 1 cut(s) 268
AfiI CCNNNNNNNGG 2 cut(s) 866, 897
AgsI TTSAA 3 cut(s) 152, 296, 305
AjnI CCWGG 1 cut(s) 170
AluBI AGCT 6 cut(s) 46, 104, 155, 277, 584, 788
AluI AGCT 6 cut(s) 46, 104, 155, 277, 584, 788
Alw21I GWGCWC 1 cut(s) 48
Alw26I GTCTC 2 cut(s) 283, 350
AlwI GGATC 5 cut(s) 186, 325, 370, 456, 603
Aor13HI TCCGGA 1 cut(s) 750
Aor51HI AGCGCT 1 cut(s) 268
AoxI GGCC 4 cut(s) 64, 271, 503, 963
ApeKI GCWGC 3 cut(s) 7, 233, 623
ApoI RAATTY 1 cut(s) 386
Asp700I GAANNNNTTC 1 cut(s) 300
AspLEI GCGC 2 cut(s) 269, 933
AspS9I GGNCC 2 cut(s) 65, 845
AsuC2I CCSGG 1 cut(s) 995
AsuHPI GGTGA 4 cut(s) 473, 590, 722, 997
AvaII GGWCC 1 cut(s) 845
BalI TGGCCA 1 cut(s) 273
BanII GRGCYC 1 cut(s) 48
BbsI GAAGAC 1 cut(s) 29
Bbv12I GWGCWC 1 cut(s) 48
BbvCI CCTCAGC 1 cut(s) 489
BbvI GCAGC 3 cut(s) 19, 245, 635
BccI CCATC 2 cut(s) 628, 818
BciT130I CCWGG 1 cut(s) 172
BclI TGATCA 1 cut(s) 795
BcnI CCSGG 1 cut(s) 995
BcoDI GTCTC 2 cut(s) 283, 350
BfaI CTAG 2 cut(s) 264, 579
BfmI CTRYAG 1 cut(s) 675
BfoI RGCGCY 1 cut(s) 270
BglII AGATCT 1 cut(s) 987
BisI GCNGC 3 cut(s) 8, 234, 624
BlsI GCNGC 3 cut(s) 9, 235, 625
Bme1390I CCNGG 2 cut(s) 172, 995
Bme18I GGWCC 1 cut(s) 845
BmgT120I GGNCC 2 cut(s) 65, 845
BmiI GGNNCC 1 cut(s) 755
BmrFI CCNGG 2 cut(s) 172, 995
BmrI ACTGGG 1 cut(s) 400
BmsI GCATC 4 cut(s) 121, 475, 742, 942
BmuI ACTGGG 1 cut(s) 400
BpiI GAAGAC 1 cut(s) 29
Bpu10I CCTNAGC 1 cut(s) 489
BpuMI CCSGG 1 cut(s) 995
BsaHI GRCGYC 2 cut(s) 92, 1011
BsaJI CCNNGG 4 cut(s) 61, 170, 444, 993
BsaWI WCCGGW 1 cut(s) 750
Bsc4I CCNNNNNNNGG 2 cut(s) 866, 897
Bse118I RCCGGY 1 cut(s) 842
Bse1I ACTGG 1 cut(s) 406
Bse3DI GCAATG 1 cut(s) 480
BseAI TCCGGA 1 cut(s) 750
BseBI CCWGG 1 cut(s) 172
BseDI CCNNGG 4 cut(s) 61, 170, 444, 993
BseGI GGATG 1 cut(s) 829
BseLI CCNNNNNNNGG 2 cut(s) 866, 897
BseMI GCAATG 1 cut(s) 480
BseMII CTCAG 2 cut(s) 480, 714
BseNI ACTGG 1 cut(s) 406
BseXI GCAGC 3 cut(s) 19, 245, 635
BseYI CCCAGC 1 cut(s) 30
BshFI GGCC 4 cut(s) 66, 273, 505, 965
BsiHKAI GWGCWC 1 cut(s) 48
BsiSI CCGG 4 cut(s) 96, 751, 843, 995
BslFI GGGAC 1 cut(s) 56
BslI CCNNNNNNNGG 2 cut(s) 866, 897
BsmAI GTCTC 2 cut(s) 283, 350
BsmFI GGGAC 1 cut(s) 56
BsnI GGCC 4 cut(s) 66, 273, 505, 965
Bsp1286I GDGCHC 1 cut(s) 48
Bsp13I TCCGGA 1 cut(s) 750
Bsp1407I TGTACA 2 cut(s) 477, 684
Bsp143I GATC 9 cut(s) 79, 178, 222, 317, 362, 448, 595, 795, 987
Bsp19I CCATGG 1 cut(s) 61
BspACI CCGC 2 cut(s) 134, 459
BspANI GGCC 4 cut(s) 66, 273, 505, 965
BspCNI CTCAG 2 cut(s) 481, 713
BspEI TCCGGA 1 cut(s) 750
BspHI TCATGA 1 cut(s) 657
BspLI GGNNCC 1 cut(s) 755
BspPI GGATC 5 cut(s) 186, 325, 370, 456, 603
BspQI GCTCTTC 1 cut(s) 921
BsrDI GCAATG 1 cut(s) 480
BsrFI RCCGGY 1 cut(s) 842
BsrGI TGTACA 2 cut(s) 477, 684
BsrI ACTGG 1 cut(s) 406
BssAI RCCGGY 1 cut(s) 842
BssECI CCNNGG 4 cut(s) 61, 170, 444, 993
BssMI GATC 9 cut(s) 79, 178, 222, 317, 362, 448, 595, 795, 987
BssNI GRCGYC 2 cut(s) 92, 1011
BssT1I CCWWGG 2 cut(s) 61, 444
Bst2UI CCWGG 1 cut(s) 172
Bst4CI ACNGT 2 cut(s) 148, 981
Bst6I CTCTTC 3 cut(s) 700, 913, 921
BstACI GRCGYC 2 cut(s) 92, 1011
BstAPI GCANNNNNTGC 1 cut(s) 839
BstAUI TGTACA 2 cut(s) 477, 684
BstC8I GCNNGC 3 cut(s) 271, 275, 903
BstDEI CTNAG 2 cut(s) 489, 700
BstDSI CCRYGG 1 cut(s) 61
BstF5I GGATG 1 cut(s) 829
BstH2I RGCGCY 1 cut(s) 270
BstHHI GCGC 2 cut(s) 269, 933
BstKTI GATC 9 cut(s) 82, 181, 225, 320, 365, 451, 598, 798, 990
BstMAI GTCTC 2 cut(s) 283, 350
BstMBI GATC 9 cut(s) 79, 178, 222, 317, 362, 448, 595, 795, 987
BstMWI GCNNNNNNNGC 2 cut(s) 839, 930
BstNI CCWGG 1 cut(s) 172
BstSCI CCNGG 2 cut(s) 170, 993
BstSFI CTRYAG 1 cut(s) 675
BstV1I GCAGC 3 cut(s) 19, 245, 635
BstV2I GAAGAC 1 cut(s) 29
BstX2I RGATCY 3 cut(s) 178, 362, 987
BstXI CCANNNNNNTGG 1 cut(s) 824
BstYI RGATCY 3 cut(s) 178, 362, 987
BsuRI GGCC 4 cut(s) 66, 273, 505, 965
BtgI CCRYGG 1 cut(s) 61
BtsCI GGATG 1 cut(s) 829
BtsIMutI CAGTG 1 cut(s) 144
Cac8I GCNNGC 3 cut(s) 271, 275, 903
CciI TCATGA 1 cut(s) 657
CfoI GCGC 2 cut(s) 269, 933
Cfr10I RCCGGY 1 cut(s) 842
Cfr13I GGNCC 2 cut(s) 65, 845
CseI GACGC 2 cut(s) 81, 215
Csp6I GTAC 3 cut(s) 478, 495, 685
CspCI CAANNNNNGTGG 4 cut(s) 789, 824, 883, 918
CviAII CATG 5 cut(s) 62, 631, 658, 877, 884
CviQI GTAC 3 cut(s) 478, 495, 685
DdeI CTNAG 2 cut(s) 489, 700
DpnI GATC 9 cut(s) 81, 180, 224, 319, 364, 450, 597, 797, 989
DpnII GATC 9 cut(s) 79, 178, 222, 317, 362, 448, 595, 795, 987
EaeI YGGCCR 2 cut(s) 271, 963
Eam1104I CTCTTC 3 cut(s) 700, 913, 921
EarI CTCTTC 3 cut(s) 700, 913, 921
Ecl136II GAGCTC 1 cut(s) 46
Eco130I CCWWGG 2 cut(s) 61, 444
Eco24I GRGCYC 1 cut(s) 48
Eco47I GGWCC 1 cut(s) 845
Eco47III AGCGCT 1 cut(s) 268
Eco53kI GAGCTC 1 cut(s) 46
Eco57I CTGAAG 2 cut(s) 71, 743
EcoICRI GAGCTC 1 cut(s) 46
EcoRI GAATTC 1 cut(s) 386
EcoRII CCWGG 1 cut(s) 170
EcoT14I CCWWGG 2 cut(s) 61, 444
EcoT22I ATGCAT 1 cut(s) 735
EcoT38I GRGCYC 1 cut(s) 48
ErhI CCWWGG 2 cut(s) 61, 444
FaeI CATG 5 cut(s) 65, 634, 661, 880, 887
FalI AAGNNNNNCTT 2 cut(s) 940, 972
FaqI GGGAC 1 cut(s) 56
FatI CATG 5 cut(s) 61, 630, 657, 876, 883
FauI CCCGC 1 cut(s) 127
FbaI TGATCA 1 cut(s) 795
Fnu4HI GCNGC 3 cut(s) 8, 234, 624
FokI GGATG 1 cut(s) 836
FriOI GRGCYC 1 cut(s) 48
Fsp4HI GCNGC 3 cut(s) 8, 234, 624
FspBI CTAG 2 cut(s) 264, 579
GlaI GCGC 2 cut(s) 268, 932
GluI GCNGC 3 cut(s) 8, 234, 624
GsaI CCCAGC 1 cut(s) 34
HaeII RGCGCY 1 cut(s) 270
HaeIII GGCC 4 cut(s) 66, 273, 505, 965
HapII CCGG 4 cut(s) 96, 751, 843, 995
HgaI GACGC 2 cut(s) 81, 215
HhaI GCGC 2 cut(s) 269, 933
Hin1I GRCGYC 2 cut(s) 92, 1011
Hin1II CATG 5 cut(s) 65, 634, 661, 880, 887
Hin6I GCGC 2 cut(s) 267, 931
HinP1I GCGC 2 cut(s) 267, 931
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HinfI GANTC 3 cut(s) 198, 513, 540
HpaII CCGG 4 cut(s) 96, 751, 843, 995
HphI GGTGA 4 cut(s) 473, 590, 722, 997
Hpy166II GTNNAC 2 cut(s) 217, 713
Hpy188I TCNGA 3 cut(s) 21, 762, 987
Hpy188III TCNNGA 6 cut(s) 315, 321, 396, 658, 702, 751
Hpy8I GTNNAC 2 cut(s) 217, 713
Hpy99I CGWCG 3 cut(s) 80, 209, 1013
HpyAV CCTTC 4 cut(s) 169, 458, 767, 992
HpyCH4III ACNGT 2 cut(s) 148, 981
HpyCH4IV ACGT 1 cut(s) 1011
HpyCH4V TGCA 7 cut(s) 58, 144, 187, 527, 733, 740, 833
HpyF10VI GCNNNNNNNGC 2 cut(s) 839, 930
HpyF3I CTNAG 2 cut(s) 489, 700
HpySE526I ACGT 1 cut(s) 1011
Hsp92I GRCGYC 2 cut(s) 92, 1011
Hsp92II CATG 5 cut(s) 65, 634, 661, 880, 887
HspAI GCGC 2 cut(s) 267, 931
Kpn2I TCCGGA 1 cut(s) 750
Ksp22I TGATCA 1 cut(s) 795
Kzo9I GATC 9 cut(s) 79, 178, 222, 317, 362, 448, 595, 795, 987
LguI GCTCTTC 1 cut(s) 921
LmnI GCTCC 4 cut(s) 4, 51, 282, 753
Lsp1109I GCAGC 3 cut(s) 19, 245, 635
LweI GCATC 4 cut(s) 121, 475, 742, 942
MaeI CTAG 2 cut(s) 264, 579
MaeII ACGT 1 cut(s) 1011
MaeIII GTNAC 4 cut(s) 413, 427, 869, 1003
MalI GATC 9 cut(s) 81, 180, 224, 319, 364, 450, 597, 797, 989
MboI GATC 9 cut(s) 79, 178, 222, 317, 362, 448, 595, 795, 987
MboII GAAGA 5 cut(s) 34, 173, 717, 930, 938
MflI RGATCY 3 cut(s) 178, 362, 987
MhlI GDGCHC 1 cut(s) 48
MlsI TGGCCA 1 cut(s) 273
MluCI AATT 4 cut(s) 306, 351, 386, 852
MluNI TGGCCA 1 cut(s) 273
MlyI GAGTC 2 cut(s) 507, 534
Mox20I TGGCCA 1 cut(s) 273
Mph1103I ATGCAT 1 cut(s) 735
MroI TCCGGA 1 cut(s) 750
MroXI GAANNNNTTC 1 cut(s) 300
MscI TGGCCA 1 cut(s) 273
MseI TTAA 2 cut(s) 126, 855
Msp20I TGGCCA 1 cut(s) 273
MspI CCGG 4 cut(s) 96, 751, 843, 995
MspR9I CCNGG 2 cut(s) 172, 995
MvaI CCWGG 1 cut(s) 172
MwoI GCNNNNNNNGC 2 cut(s) 839, 930
NciI CCSGG 1 cut(s) 995
NcoI CCATGG 1 cut(s) 61
NdeII GATC 9 cut(s) 79, 178, 222, 317, 362, 448, 595, 795, 987
NlaIII CATG 5 cut(s) 65, 634, 661, 880, 887
NlaIV GGNNCC 1 cut(s) 755
NmuCI GTSAC 2 cut(s) 413, 1003
NsiI ATGCAT 1 cut(s) 735
PagI TCATGA 1 cut(s) 657
PciSI GCTCTTC 1 cut(s) 921
PdmI GAANNNNTTC 1 cut(s) 300
PfeI GAWTC 1 cut(s) 198
PkrI GCNGC 3 cut(s) 9, 235, 625
PleI GAGTC 2 cut(s) 507, 534
PpsI GAGTC 2 cut(s) 507, 534
PsiI TTATAA 1 cut(s) 588
Psp124BI GAGCTC 1 cut(s) 48
Psp6I CCWGG 1 cut(s) 170
PspFI CCCAGC 1 cut(s) 30
PspGI CCWGG 1 cut(s) 170
PspN4I GGNNCC 1 cut(s) 755
PspPI GGNCC 2 cut(s) 65, 845
PsuI RGATCY 3 cut(s) 178, 362, 987
RsaI GTAC 3 cut(s) 479, 496, 686
RsaNI GTAC 3 cut(s) 478, 495, 685
SacI GAGCTC 1 cut(s) 48
SapI GCTCTTC 1 cut(s) 921
SaqAI TTAA 2 cut(s) 126, 855
SatI GCNGC 3 cut(s) 8, 234, 624
Sau3AI GATC 9 cut(s) 79, 178, 222, 317, 362, 448, 595, 795, 987
Sau96I GGNCC 2 cut(s) 65, 845
SchI GAGTC 2 cut(s) 507, 534
ScrFI CCNGG 2 cut(s) 172, 995
SduI GDGCHC 1 cut(s) 48
SfaNI GCATC 4 cut(s) 121, 475, 742, 942
SfcI CTRYAG 1 cut(s) 675
SinI GGWCC 1 cut(s) 845
Sse9I AATT 4 cut(s) 306, 351, 386, 852
SsiI CCGC 2 cut(s) 134, 459
SspMI CTAG 2 cut(s) 264, 579
SstI GAGCTC 1 cut(s) 48
StyD4I CCNGG 2 cut(s) 170, 993
StyI CCWWGG 2 cut(s) 61, 444
TaaI ACNGT 2 cut(s) 148, 981
TaiI ACGT 1 cut(s) 1014
TaqI TCGA 3 cut(s) 78, 228, 618
TasI AATT 4 cut(s) 306, 351, 386, 852
TatI WGTACW 2 cut(s) 477, 684
TfiI GAWTC 1 cut(s) 198
Tru1I TTAA 2 cut(s) 126, 855
Tru9I TTAA 2 cut(s) 126, 855
TscAI CASTG 1 cut(s) 151
TseFI GTSAC 2 cut(s) 413, 1003
TseI GCWGC 3 cut(s) 7, 233, 623
Tsp45I GTSAC 2 cut(s) 413, 1003
TspGWI ACGGA 2 cut(s) 380, 608
TspRI CASTG 1 cut(s) 151
VpaK11BI GGWCC 1 cut(s) 845
XapI RAATTY 1 cut(s) 386
XmnI GAANNNNTTC 1 cut(s) 300
XspI CTAG 2 cut(s) 264, 579
ZraI GACGTC 1 cut(s) 1012
Zsp2I ATGCAT 1 cut(s) 735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.