Rmu_sc0005996.1_g000022

Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Unwinds duplex DNA. Component of the meiotic recombination pathway. Seems to play a role in mediating chromosome homology search, chromosome pairing and synapsis at early stages and probably chromosome crossing-over at later stages in meiosis. Probably is involved in the repair of meiotic double strand breaks (DBSs) and in homologous recombination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005996.1
OG0014309
Physical Location & Seq
Reverse (-)
102014 .. 104908
2895 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005996.1_g000022.1.cds

Sequence Viewer

Length: 1107 bp
atggagcaacagagaacggttcagcagcaagatgaggtcgaagaaatgcagcacggccctgttcccgtcgagcagcttcaggtaaatccagttgaaattacagtcgatttcgagcaattgaggggcttcccgatctgggttattgttcaatttggttatgatggggttattgttcggattcgcattgcttcccttgatgtgaagaagctcaaagatgcaggactttgcacggttgaatctgttgcttactcaccaaggaaagatcttctgcaaatcaaaggaatcagtgaagctaaagttgacaagatcattgaagcagcctccaaactggtgcctttgggttttactagtgctagtcagcttcatgctcagagactcgaaatcattcaaatcacttctggatcaagagaacttgataagatattggagggaggaatcgagactggatctattactgagatatatggtgagttccgctccggaaagactcagctgtgccacacactttgtgtcacttgccaactccctttagatcaaggaggtggtgagggaaaagcaatgtacattgatgctgagggtacattcaggcctcagagactcttacagatagcagagaggtttggactcaacggtgctgatgttttggaaaacgttgcctatgctagagcatataacactgatcatcaatcaaggctcctgcttgaagcagcttcaatgatggtagaaacaaggtttgctctcatgatagtagacagtgccacagccctctacagaacagatttctccggaagaggagaactctcagcacggcagatgcatcttgcaaaatttctccggagtcttcagaagttagcagatgagtttggtgtggctgttgttatcacaaatcaagtagttgcacaagtggatggttctgcactctttgctgggcctcaaattaagcctattggtggtaacatcatggcccacgcctctacgacaaggcttgctgtccggaagggaagaggggaggagcgcatttgtaaagtaatcagctctccttgtctggctgaagcagaagcacggtttcagatatctccagaaggcgtcacggatgtgaaggactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000150 GO:0000217 GO:0000228 GO:0000280 GO:0000400 GO:0000724 GO:0000725 GO:0000730 GO:0000793 GO:0000794 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006312 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0008094 GO:0008150 GO:0008152 GO:0009314 GO:0009628 GO:0009889 GO:0009987 GO:0010212 GO:0010332 GO:0010468 GO:0010556 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0022402 GO:0022414 GO:0022607 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0033554 GO:0034622 GO:0034641 GO:0035825 GO:0042148 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045003 GO:0046483 GO:0048285 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051276 GO:0051321 GO:0051716 GO:0060255 GO:0061982 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070192 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090735 GO:0097159 GO:0140013 GO:0140097 GO:1901360 GO:1901363 GO:1903046 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03440 ko03460 ko05200 ko05212 map03440 map03460 map05200 map05212
Pfam Domains
Protein Families
HHH_5 Rad51

Protein Analysis

368

Amino Acids

40.39

Weight (kDa)

5.56

Isoelectric Point (pI)

35.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014309)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20850 AT5G20850
fragaria_vesca FvH4_1g09550 FvH4_1g09550
malus_domestica MD15G1224500.v1.1
prunus_persica Prupe.7G190800_v2.0.a1 Prupe.7G190800_v2.0.a1
pyrus_communis pycom15g19970
rosa_chinensis RchiOBHm_Chr2g0096301
rosa_laevigata RLG00000016633
rosa_multiflora Rmu_sc0005996.1_g000022
rosa_roxburghii Rroxscaffold_2G00145680
rosa_rugosa Rorug02G0057600
rosa_samantha Rh2AG104400 Rh2BG106500 Rh2CG108400
rosa_wichuraiana Rw2G008040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 331
AccBSI CCGCTC 1 cut(s) 477
AccI GTMKAC 1 cut(s) 750
AccIII TCCGGA 4 cut(s) 479, 785, 834, 993
AciI CCGC 1 cut(s) 475
AclI AACGTT 1 cut(s) 651
AclWI GGATC 2 cut(s) 409, 454
AcsI RAATTY 1 cut(s) 827
AcuI CTGAAG 3 cut(s) 62, 827, 1071
AcyI GRCGYC 1 cut(s) 1086
AdeI CACNNNGTG 1 cut(s) 509
AfaI GTAC 2 cut(s) 563, 580
AfiI CCNNNNNNNGG 2 cut(s) 136, 950
AgsI TTSAA 7 cut(s) 95, 149, 236, 314, 389, 704, 714
AhlI ACTAGT 1 cut(s) 347
AleI CACNNNNGTG 1 cut(s) 1094
AluBI AGCT 7 cut(s) 76, 208, 293, 361, 493, 710, 1035
AluI AGCT 7 cut(s) 76, 208, 293, 361, 493, 710, 1035
Alw26I GTCTC 3 cut(s) 367, 434, 589
AlwI GGATC 2 cut(s) 409, 454
Aor13HI TCCGGA 4 cut(s) 479, 785, 834, 993
AoxI GGCC 4 cut(s) 55, 587, 929, 963
ApeKI GCWGC 5 cut(s) 25, 49, 73, 317, 707
ApoI RAATTY 1 cut(s) 827
Asp700I GAANNNNTTC 1 cut(s) 384
AspLEI GCGC 1 cut(s) 1017
AspS9I GGNCC 3 cut(s) 56, 929, 964
AsuHPI GGTGA 3 cut(s) 243, 479, 557
BanI GGYRCC 1 cut(s) 331
BbsI GAAGAC 1 cut(s) 833
BbvCI CCTCAGC 1 cut(s) 573
BbvI GCAGC 5 cut(s) 37, 61, 85, 329, 719
BccI CCATC 3 cut(s) 155, 712, 902
BceAI ACGGC 2 cut(s) 70, 824
BclI TGATCA 1 cut(s) 679
BcoDI GTCTC 3 cut(s) 367, 434, 589
BcuI ACTAGT 1 cut(s) 347
BfaI CTAG 3 cut(s) 348, 354, 663
BfmI CTRYAG 1 cut(s) 769
BglII AGATCT 1 cut(s) 262
BisI GCNGC 5 cut(s) 26, 50, 74, 318, 708
BlsI GCNGC 5 cut(s) 27, 51, 75, 319, 709
BmgT120I GGNCC 3 cut(s) 56, 929, 964
BmiI GGNNCC 2 cut(s) 333, 695
BmsI GCATC 4 cut(s) 205, 559, 804, 826
BpiI GAAGAC 1 cut(s) 833
BplI GAGNNNNNCTC 4 cut(s) 461, 493, 783, 815
BpmI CTGGAG 1 cut(s) 1062
Bpu10I CCTNAGC 1 cut(s) 573
BsaHI GRCGYC 1 cut(s) 1086
BsaJI CCNNGG 1 cut(s) 254
BsaWI WCCGGW 4 cut(s) 479, 785, 834, 993
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 950
Bse1I ACTGG 3 cut(s) 89, 333, 448
Bse3DI GCAATG 2 cut(s) 183, 564
BseAI TCCGGA 4 cut(s) 479, 785, 834, 993
BseDI CCNNGG 1 cut(s) 254
BseGI GGATG 2 cut(s) 913, 1099
BseLI CCNNNNNNNGG 2 cut(s) 136, 950
BseMI GCAATG 2 cut(s) 183, 564
BseMII CTCAG 6 cut(s) 383, 447, 503, 564, 605, 816
BseNI ACTGG 3 cut(s) 89, 333, 448
BseRI GAGGAG 2 cut(s) 807, 1025
BseXI GCAGC 5 cut(s) 37, 61, 85, 329, 719
BseYI CCCAGC 1 cut(s) 926
BsgI GTGCAG 1 cut(s) 900
BshFI GGCC 4 cut(s) 57, 589, 931, 965
BshNI GGYRCC 1 cut(s) 331
BsiSI CCGG 4 cut(s) 480, 786, 835, 994
BslI CCNNNNNNNGG 2 cut(s) 136, 950
BsmAI GTCTC 3 cut(s) 367, 434, 589
BsnI GGCC 4 cut(s) 57, 589, 931, 965
Bsp13I TCCGGA 4 cut(s) 479, 785, 834, 993
Bsp1407I TGTACA 1 cut(s) 561
Bsp143I GATC 7 cut(s) 132, 262, 306, 401, 446, 532, 679
BspACI CCGC 1 cut(s) 475
BspANI GGCC 4 cut(s) 57, 589, 931, 965
BspCNI CTCAG 6 cut(s) 382, 448, 502, 565, 604, 815
BspEI TCCGGA 4 cut(s) 479, 785, 834, 993
BspHI TCATGA 1 cut(s) 741
BspLI GGNNCC 2 cut(s) 333, 695
BspPI GGATC 2 cut(s) 409, 454
BspT107I GGYRCC 1 cut(s) 331
BsrBI CCGCTC 1 cut(s) 477
BsrDI GCAATG 2 cut(s) 183, 564
BsrGI TGTACA 1 cut(s) 561
BsrI ACTGG 3 cut(s) 89, 333, 448
BssECI CCNNGG 1 cut(s) 254
BssMI GATC 7 cut(s) 132, 262, 306, 401, 446, 532, 679
BssNI GRCGYC 1 cut(s) 1086
BssT1I CCWWGG 1 cut(s) 254
Bst4CI ACNGT 6 cut(s) 19, 103, 232, 632, 755, 1065
Bst6I CTCTTC 2 cut(s) 784, 997
BstACI GRCGYC 1 cut(s) 1086
BstAPI GCANNNNNTGC 1 cut(s) 923
BstAUI TGTACA 1 cut(s) 561
BstC8I GCNNGC 1 cut(s) 987
BstDEI CTNAG 6 cut(s) 369, 456, 489, 573, 591, 802
BstF5I GGATG 2 cut(s) 913, 1099
BstHHI GCGC 1 cut(s) 1017
BstKTI GATC 7 cut(s) 135, 265, 309, 404, 449, 535, 682
BstMAI GTCTC 3 cut(s) 367, 434, 589
BstMBI GATC 7 cut(s) 132, 262, 306, 401, 446, 532, 679
BstMWI GCNNNNNNNGC 1 cut(s) 923
BstSFI CTRYAG 1 cut(s) 769
BstV1I GCAGC 5 cut(s) 37, 61, 85, 329, 719
BstV2I GAAGAC 1 cut(s) 833
BstX2I RGATCY 2 cut(s) 262, 446
BstYI RGATCY 2 cut(s) 262, 446
BsuRI GGCC 4 cut(s) 57, 589, 931, 965
BtsCI GGATG 2 cut(s) 913, 1099
BtsIMutI CAGTG 3 cut(s) 292, 675, 760
Cac8I GCNNGC 1 cut(s) 987
CciI TCATGA 1 cut(s) 741
CfoI GCGC 1 cut(s) 1017
Cfr13I GGNCC 3 cut(s) 56, 929, 964
CseI GACGC 1 cut(s) 1075
Csp6I GTAC 2 cut(s) 562, 579
CspCI CAANNNNNGTGG 2 cut(s) 488, 523
CviAII CATG 3 cut(s) 365, 742, 961
CviQI GTAC 2 cut(s) 562, 579
DdeI CTNAG 6 cut(s) 369, 456, 489, 573, 591, 802
DpnI GATC 7 cut(s) 134, 264, 308, 403, 448, 534, 681
DpnII GATC 7 cut(s) 132, 262, 306, 401, 446, 532, 679
DraIII CACNNNGTG 1 cut(s) 509
Eam1104I CTCTTC 2 cut(s) 784, 997
EarI CTCTTC 2 cut(s) 784, 997
Eco130I CCWWGG 1 cut(s) 254
Eco147I AGGCCT 1 cut(s) 589
Eco32I GATATC 1 cut(s) 1074
Eco57I CTGAAG 3 cut(s) 62, 827, 1071
EcoRV GATATC 1 cut(s) 1074
EcoT14I CCWWGG 1 cut(s) 254
EcoT22I ATGCAT 1 cut(s) 819
ErhI CCWWGG 1 cut(s) 254
FaeI CATG 3 cut(s) 368, 745, 964
FaiI YATR 9 cut(s) 159, 366, 463, 465, 660, 670, 672, 743, 962
FatI CATG 3 cut(s) 364, 741, 960
FbaI TGATCA 1 cut(s) 679
FblI GTMKAC 1 cut(s) 750
Fnu4HI GCNGC 5 cut(s) 26, 50, 74, 318, 708
FokI GGATG 1 cut(s) 920
Fsp4HI GCNGC 5 cut(s) 26, 50, 74, 318, 708
FspBI CTAG 3 cut(s) 348, 354, 663
GlaI GCGC 1 cut(s) 1016
GluI GCNGC 5 cut(s) 26, 50, 74, 318, 708
GsaI CCCAGC 1 cut(s) 930
GsuI CTGGAG 1 cut(s) 1062
HaeIII GGCC 4 cut(s) 57, 589, 931, 965
HapII CCGG 4 cut(s) 480, 786, 835, 994
HgaI GACGC 1 cut(s) 1075
HhaI GCGC 1 cut(s) 1017
Hin1I GRCGYC 1 cut(s) 1086
Hin1II CATG 3 cut(s) 368, 745, 964
Hin6I GCGC 1 cut(s) 1015
HinP1I GCGC 1 cut(s) 1015
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HinfI GANTC 9 cut(s) 178, 236, 282, 375, 435, 487, 597, 624, 838
HpaII CCGG 4 cut(s) 480, 786, 835, 994
HphI GGTGA 3 cut(s) 243, 479, 557
Hpy166II GTNNAC 2 cut(s) 301, 751
Hpy188I TCNGA 5 cut(s) 177, 372, 594, 846, 1071
Hpy8I GTNNAC 2 cut(s) 301, 751
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 3 cut(s) 991, 1076, 1093
HpyCH4III ACNGT 6 cut(s) 19, 103, 232, 632, 755, 1065
HpyCH4IV ACGT 1 cut(s) 651
HpyCH4V TGCA 8 cut(s) 49, 218, 228, 271, 817, 824, 899, 917
HpyF10VI GCNNNNNNNGC 1 cut(s) 923
HpyF3I CTNAG 6 cut(s) 369, 456, 489, 573, 591, 802
HpySE526I ACGT 1 cut(s) 651
Hsp92I GRCGYC 1 cut(s) 1086
Hsp92II CATG 3 cut(s) 368, 745, 964
HspAI GCGC 1 cut(s) 1015
Kpn2I TCCGGA 4 cut(s) 479, 785, 834, 993
Ksp22I TGATCA 1 cut(s) 679
Kzo9I GATC 7 cut(s) 132, 262, 306, 401, 446, 532, 679
LmnI GCTCC 4 cut(s) 4, 482, 699, 1012
Lsp1109I GCAGC 5 cut(s) 37, 61, 85, 329, 719
LweI GCATC 4 cut(s) 205, 559, 804, 826
MaeI CTAG 3 cut(s) 348, 354, 663
MaeII ACGT 1 cut(s) 651
MaeIII GTNAC 3 cut(s) 511, 953, 1087
MalI GATC 7 cut(s) 134, 264, 308, 403, 448, 534, 681
MbiI CCGCTC 1 cut(s) 477
MboI GATC 7 cut(s) 132, 262, 306, 401, 446, 532, 679
MboII GAAGA 6 cut(s) 53, 214, 257, 801, 833, 1014
MfeI CAATTG 1 cut(s) 116
MflI RGATCY 2 cut(s) 262, 446
MluCI AATT 5 cut(s) 96, 116, 149, 827, 936
MlyI GAGTC 5 cut(s) 369, 481, 591, 618, 847
Mph1103I ATGCAT 1 cut(s) 819
MroI TCCGGA 4 cut(s) 479, 785, 834, 993
MroXI GAANNNNTTC 1 cut(s) 384
MseI TTAA 1 cut(s) 939
MslI CAYNNNNRTG 1 cut(s) 1094
MspA1I CMGCKG 1 cut(s) 493
MspI CCGG 4 cut(s) 480, 786, 835, 994
MunI CAATTG 1 cut(s) 116
MwoI GCNNNNNNNGC 1 cut(s) 923
NdeII GATC 7 cut(s) 132, 262, 306, 401, 446, 532, 679
NlaIII CATG 3 cut(s) 368, 745, 964
NlaIV GGNNCC 2 cut(s) 333, 695
NmuCI GTSAC 2 cut(s) 511, 1087
NsiI ATGCAT 1 cut(s) 819
OliI CACNNNNGTG 1 cut(s) 1094
PagI TCATGA 1 cut(s) 741
PceI AGGCCT 1 cut(s) 589
PdmI GAANNNNTTC 1 cut(s) 384
PfeI GAWTC 4 cut(s) 178, 236, 282, 435
PkrI GCNGC 5 cut(s) 27, 51, 75, 319, 709
PleI GAGTC 5 cut(s) 369, 481, 591, 618, 846
PpsI GAGTC 5 cut(s) 369, 481, 591, 618, 846
Psp1406I AACGTT 1 cut(s) 651
PspFI CCCAGC 1 cut(s) 926
PspN4I GGNNCC 2 cut(s) 333, 695
PspPI GGNCC 3 cut(s) 56, 929, 964
PsuI RGATCY 2 cut(s) 262, 446
PvuII CAGCTG 1 cut(s) 493
RsaI GTAC 2 cut(s) 563, 580
RsaNI GTAC 2 cut(s) 562, 579
RseI CAYNNNNRTG 1 cut(s) 1094
SaqAI TTAA 1 cut(s) 939
SatI GCNGC 5 cut(s) 26, 50, 74, 318, 708
Sau3AI GATC 7 cut(s) 132, 262, 306, 401, 446, 532, 679
Sau96I GGNCC 3 cut(s) 56, 929, 964
SchI GAGTC 5 cut(s) 369, 481, 591, 618, 847
SfaNI GCATC 4 cut(s) 205, 559, 804, 826
SfcI CTRYAG 1 cut(s) 769
SmiMI CAYNNNNRTG 1 cut(s) 1094
SpeI ACTAGT 1 cut(s) 347
Sse9I AATT 5 cut(s) 96, 116, 149, 827, 936
SseBI AGGCCT 1 cut(s) 589
SsiI CCGC 1 cut(s) 475
SspMI CTAG 3 cut(s) 348, 354, 663
StuI AGGCCT 1 cut(s) 589
StyI CCWWGG 1 cut(s) 254
TaaI ACNGT 6 cut(s) 19, 103, 232, 632, 755, 1065
TaiI ACGT 1 cut(s) 654
TaqI TCGA 6 cut(s) 39, 69, 105, 111, 378, 438
TasI AATT 5 cut(s) 96, 116, 149, 827, 936
TatI WGTACW 1 cut(s) 561
TfiI GAWTC 4 cut(s) 178, 236, 282, 435
Tru1I TTAA 1 cut(s) 939
Tru9I TTAA 1 cut(s) 939
TscAI CASTG 3 cut(s) 292, 682, 760
TseFI GTSAC 2 cut(s) 511, 1087
TseI GCWGC 5 cut(s) 25, 49, 73, 317, 707
Tsp45I GTSAC 2 cut(s) 511, 1087
TspDTI ATGAA 1 cut(s) 353
TspGWI ACGGA 1 cut(s) 1106
TspRI CASTG 3 cut(s) 292, 682, 760
XapI RAATTY 1 cut(s) 827
XmiI GTMKAC 1 cut(s) 750
XmnI GAANNNNTTC 1 cut(s) 384
XspI CTAG 3 cut(s) 348, 354, 663
Zsp2I ATGCAT 1 cut(s) 819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.