MD15G1241800.v1.1

GDSL esterase lipase EXL3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0028417
Physical Location & Seq
Reverse (-)
19919311 .. 19925437
6127 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1241800.v1.1.491

Sequence Viewer

Length: 1014 bp
ATGAAGTTTCTCTCCCAAAACCTTATTCACTCGTCATTGAAATTTTCCCTCAAAATTTTTCTCATCGTTATAGTTTTATTCTTCTACAACAATGTTGCGGCCGTAAACCTACCAAACAACGAAAAGGTTCCGGCAGTGATTGTTTTCGGAGATTCTGTAGTGGATCCTGGAAACAACAACTATATTAGCACTATAGTCAAATGTAATTTCGCACCATATGAGAGGGACTTTGTAGGAAAAAGACCTACTGGAAGGTTTAGCAATGGCAGAGTCCCCTCAGACATGATAGCTGAATCAGTTGGAGTAAAGAAGATATTGCCAGCTTATCTGGATCCAAATCTGAAGATTCATGACTTACTTACAAGTGTAAGTTTTGCCTCAGGTTGCTCAGGATATGATCCTCTCACTCCCAAAATAGTGTCTGTCATGTCATTATCAGATCAAATAGACATGTTCAAAAATTATATAAGCAAGATTACGGTAGCAGTCAGAAAGGAGGGCGCTGCAACTATAGTATCGAAAAGCATATACATTGTGTACATAGGAAGTGACGACATTGCGCATACCTATTATTTTACACCACTAAGGAGACCTCGGTATGACATTTCAGCATATACCGATCTCATGGTTGAATCAGCTTCAAGTTTCTTTCAGGATTTGGCCTTCGTGTTCTTGTTGACTTTCGAAGTTGAGATTGTGGAGCTTGTCTACCTTGATATCTATAACACATTGCTTTCCCTCATCCAAAACCCTACTCAATATGGATTTGAAGTGATGGATCAAGGATGTTGTGGAACAGAAAATATTGAGGTTAGCATTTTGTGTACTCGTTACTCTCCGGGAACCTGCAACGATTCATCAAAATACATATTCTGGGACAGCTACCATCCTTCAGAGAAGGGATATGAAGTAATAATCCCTCTGGTTTTCAATTCGCAAGCAATCAATAAATTTCCTTTTAGTTTTGGTGTATTTGCTTTCAGAAGTTTGAGCCTCAATTTGTTAATAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

338

Amino Acids

37.84

Weight (kDa)

5.63

Isoelectric Point (pI)

29.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 46 - 194 1.9e-08 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028417)

Species Orthologous Gene IDs
malus_domestica MD15G1241800.v1.1
pyrus_communis pycom15g21330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 561
Acc36I ACCTGC 1 cut(s) 854
AccI GTMKAC 1 cut(s) 708
AciI CCGC 1 cut(s) 98
AclWI GGATC 6 cut(s) 158, 171, 326, 339, 392, 786
AcoI YGGCCR 1 cut(s) 99
AcsI RAATTY 3 cut(s) 41, 54, 950
AcuI CTGAAG 2 cut(s) 362, 876
AfaI GTAC 2 cut(s) 539, 826
AflIII ACRYGT 1 cut(s) 450
AgsI TTSAA 6 cut(s) 40, 457, 632, 642, 770, 931
AjnI CCWGG 1 cut(s) 166
AjuI GAANNNNNNNTTGG 2 cut(s) 9, 41
AluBI AGCT 5 cut(s) 290, 323, 638, 703, 882
AluI AGCT 5 cut(s) 290, 323, 638, 703, 882
Alw26I GTCTC 1 cut(s) 583
AlwI GGATC 6 cut(s) 158, 171, 326, 339, 392, 786
AoxI GGCC 2 cut(s) 99, 660
ApeKI GCWGC 1 cut(s) 503
ApoI RAATTY 3 cut(s) 41, 54, 950
Asp700I GAANNNNTTC 1 cut(s) 126
AspLEI GCGC 2 cut(s) 503, 562
AsuC2I CCSGG 1 cut(s) 840
AsuII TTCGAA 1 cut(s) 684
AxyI CCTNAGG 1 cut(s) 379
BaeI ACNNNNGTAYC 2 cut(s) 498, 531
BamHI GGATCC 2 cut(s) 163, 331
BbvI GCAGC 1 cut(s) 490
BccI CCATC 2 cut(s) 769, 894
BceAI ACGGC 1 cut(s) 86
BciT130I CCWGG 1 cut(s) 168
BcnI CCSGG 1 cut(s) 840
BcoDI GTCTC 1 cut(s) 583
BfaI CTAG 1 cut(s) 1012
BfmI CTRYAG 3 cut(s) 156, 192, 510
BfoI RGCGCY 1 cut(s) 504
BfuAI ACCTGC 1 cut(s) 854
BisI GCNGC 2 cut(s) 99, 504
BlsI GCNGC 2 cut(s) 100, 505
Bme1390I CCNGG 2 cut(s) 168, 840
BmiI GGNNCC 4 cut(s) 129, 165, 333, 844
BmrFI CCNGG 2 cut(s) 168, 840
Bpu10I CCTNAGC 1 cut(s) 388
Bpu14I TTCGAA 1 cut(s) 684
BpuMI CCSGG 1 cut(s) 840
BsaBI GATNNNNATC 1 cut(s) 336
BsaI GGTCTC 1 cut(s) 583
BsaJI CCNNGG 1 cut(s) 593
BsaXI ACNNNNNCTCC 2 cut(s) 141, 171
Bse1I ACTGG 1 cut(s) 253
Bse21I CCTNAGG 1 cut(s) 379
Bse3DI GCAATG 3 cut(s) 268, 555, 728
Bse8I GATNNNNATC 1 cut(s) 336
BseBI CCWGG 1 cut(s) 168
BseDI CCNNGG 1 cut(s) 593
BseGI GGATG 3 cut(s) 741, 791, 886
BseJI GATNNNNATC 1 cut(s) 336
BseMI GCAATG 3 cut(s) 268, 555, 728
BseMII CTCAG 3 cut(s) 291, 393, 402
BseNI ACTGG 1 cut(s) 253
BseX3I CGGCCG 1 cut(s) 99
BseXI GCAGC 1 cut(s) 490
Bsh1285I CGRYCG 1 cut(s) 102
BshFI GGCC 2 cut(s) 101, 662
BsiEI CGRYCG 1 cut(s) 102
BsiSI CCGG 2 cut(s) 131, 839
BslFI GGGAC 3 cut(s) 239, 257, 890
BsmAI GTCTC 1 cut(s) 583
BsmFI GGGAC 3 cut(s) 239, 257, 890
BsnI GGCC 2 cut(s) 101, 662
Bso31I GGTCTC 1 cut(s) 583
Bsp119I TTCGAA 1 cut(s) 684
Bsp1407I TGTACA 1 cut(s) 537
Bsp143I GATC 6 cut(s) 163, 331, 397, 439, 619, 778
BspACI CCGC 1 cut(s) 98
BspANI GGCC 2 cut(s) 101, 662
BspCNI CTCAG 3 cut(s) 290, 392, 401
BspHI TCATGA 1 cut(s) 349
BspLI GGNNCC 4 cut(s) 129, 165, 333, 844
BspMI ACCTGC 1 cut(s) 854
BspPI GGATC 6 cut(s) 158, 171, 326, 339, 392, 786
BspT104I TTCGAA 1 cut(s) 684
BspTNI GGTCTC 1 cut(s) 583
BsrDI GCAATG 3 cut(s) 268, 555, 728
BsrGI TGTACA 1 cut(s) 537
BsrI ACTGG 1 cut(s) 253
BssECI CCNNGG 1 cut(s) 593
BssMI GATC 6 cut(s) 163, 331, 397, 439, 619, 778
Bst2UI CCWGG 1 cut(s) 168
Bst4CI ACNGT 1 cut(s) 481
BstAUI TGTACA 1 cut(s) 537
BstBI TTCGAA 1 cut(s) 684
BstC8I GCNNGC 2 cut(s) 321, 939
BstDEI CTNAG 4 cut(s) 277, 379, 388, 584
BstF5I GGATG 3 cut(s) 741, 791, 886
BstH2I RGCGCY 1 cut(s) 504
BstHHI GCGC 2 cut(s) 503, 562
BstKTI GATC 6 cut(s) 166, 334, 400, 442, 622, 781
BstMAI GTCTC 1 cut(s) 583
BstMBI GATC 6 cut(s) 163, 331, 397, 439, 619, 778
BstMCI CGRYCG 1 cut(s) 102
BstNI CCWGG 1 cut(s) 168
BstNSI RCATGY 1 cut(s) 454
BstSCI CCNGG 2 cut(s) 166, 838
BstSFI CTRYAG 3 cut(s) 156, 192, 510
BstV1I GCAGC 1 cut(s) 490
BstX2I RGATCY 2 cut(s) 163, 331
BstYI RGATCY 2 cut(s) 163, 331
BstZI CGGCCG 1 cut(s) 99
Bsu36I CCTNAGG 1 cut(s) 379
BsuRI GGCC 2 cut(s) 101, 662
BtsCI GGATG 3 cut(s) 741, 791, 886
BtsI GCAGTG 1 cut(s) 141
BtsIMutI CAGTG 1 cut(s) 141
BveI ACCTGC 1 cut(s) 854
Cac8I GCNNGC 2 cut(s) 321, 939
CciI TCATGA 1 cut(s) 349
CfoI GCGC 2 cut(s) 503, 562
Csp6I GTAC 2 cut(s) 538, 825
CviAII CATG 5 cut(s) 283, 350, 427, 451, 625
CviJI RGCY 8 cut(s) 101, 290, 323, 638, 662, 703, 882, 993
CviKI_1 RGCY 8 cut(s) 101, 290, 323, 638, 662, 703, 882, 993
CviQI GTAC 2 cut(s) 538, 825
DdeI CTNAG 4 cut(s) 277, 379, 388, 584
DpnI GATC 6 cut(s) 165, 333, 399, 441, 621, 780
DpnII GATC 6 cut(s) 163, 331, 397, 439, 619, 778
EaeI YGGCCR 1 cut(s) 99
EagI CGGCCG 1 cut(s) 99
EclXI CGGCCG 1 cut(s) 99
Eco31I GGTCTC 1 cut(s) 583
Eco32I GATATC 1 cut(s) 718
Eco52I CGGCCG 1 cut(s) 99
Eco57I CTGAAG 2 cut(s) 362, 876
Eco81I CCTNAGG 1 cut(s) 379
EcoRII CCWGG 1 cut(s) 166
EcoRV GATATC 1 cut(s) 718
FaeI CATG 5 cut(s) 286, 353, 430, 454, 628
FaqI GGGAC 3 cut(s) 239, 257, 890
FatI CATG 5 cut(s) 282, 349, 426, 450, 624
FauNDI CATATG 1 cut(s) 217
FblI GTMKAC 1 cut(s) 708
Fnu4HI GCNGC 2 cut(s) 99, 504
FokI GGATG 3 cut(s) 728, 798, 873
Fsp4HI GCNGC 2 cut(s) 99, 504
FspBI CTAG 1 cut(s) 1012
FspI TGCGCA 1 cut(s) 561
GlaI GCGC 2 cut(s) 502, 561
GluI GCNGC 2 cut(s) 99, 504
HaeII RGCGCY 1 cut(s) 504
HaeIII GGCC 2 cut(s) 101, 662
HapII CCGG 2 cut(s) 131, 839
HhaI GCGC 2 cut(s) 503, 562
Hin1II CATG 5 cut(s) 286, 353, 430, 454, 628
Hin6I GCGC 2 cut(s) 501, 560
HinP1I GCGC 2 cut(s) 501, 560
HincII GTYRAC 1 cut(s) 678
HindII GTYRAC 1 cut(s) 678
HinfI GANTC 6 cut(s) 152, 270, 293, 346, 632, 854
HpaII CCGG 2 cut(s) 131, 839
Hpy166II GTNNAC 5 cut(s) 106, 538, 678, 709, 825
Hpy188I TCNGA 7 cut(s) 149, 280, 342, 439, 491, 895, 983
Hpy188III TCNNGA 4 cut(s) 329, 350, 390, 653
Hpy8I GTNNAC 5 cut(s) 106, 538, 678, 709, 825
HpyAV CCTTC 4 cut(s) 246, 673, 892, 900
HpyCH4III ACNGT 1 cut(s) 481
HpyCH4V TGCA 2 cut(s) 506, 849
HpyF3I CTNAG 4 cut(s) 277, 379, 388, 584
Hsp92II CATG 5 cut(s) 286, 353, 430, 454, 628
HspAI GCGC 2 cut(s) 501, 560
Kzo9I GATC 6 cut(s) 163, 331, 397, 439, 619, 778
LmnI GCTCC 1 cut(s) 700
Lsp1109I GCAGC 1 cut(s) 490
MaeI CTAG 1 cut(s) 1012
MaeIII GTNAC 2 cut(s) 548, 830
MalI GATC 6 cut(s) 165, 333, 399, 441, 621, 780
MboI GATC 6 cut(s) 163, 331, 397, 439, 619, 778
MboII GAAGA 3 cut(s) 73, 322, 355
MflI RGATCY 2 cut(s) 163, 331
MluCI AATT 7 cut(s) 41, 54, 205, 460, 931, 950, 997
MlyI GAGTC 1 cut(s) 279
MmeI TCCRAC 1 cut(s) 280
MroXI GAANNNNTTC 1 cut(s) 126
MseI TTAA 1 cut(s) 1004
MspI CCGG 2 cut(s) 131, 839
MspR9I CCNGG 2 cut(s) 168, 840
MvaI CCWGG 1 cut(s) 168
NciI CCSGG 1 cut(s) 840
NdeI CATATG 1 cut(s) 217
NdeII GATC 6 cut(s) 163, 331, 397, 439, 619, 778
NlaIII CATG 5 cut(s) 286, 353, 430, 454, 628
NlaIV GGNNCC 4 cut(s) 129, 165, 333, 844
NmuCI GTSAC 1 cut(s) 548
NsbI TGCGCA 1 cut(s) 561
NspI RCATGY 1 cut(s) 454
NspV TTCGAA 1 cut(s) 684
PagI TCATGA 1 cut(s) 349
PciI ACATGT 1 cut(s) 450
PdmI GAANNNNTTC 1 cut(s) 126
PfeI GAWTC 5 cut(s) 152, 293, 346, 632, 854
PfoI TCCNGGA 2 cut(s) 166, 838
PkrI GCNGC 2 cut(s) 100, 505
PleI GAGTC 1 cut(s) 278
PpsI GAGTC 1 cut(s) 278
PscI ACATGT 1 cut(s) 450
Psp6I CCWGG 1 cut(s) 166
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 4 cut(s) 129, 165, 333, 844
PsuI RGATCY 2 cut(s) 163, 331
RsaI GTAC 2 cut(s) 539, 826
RsaNI GTAC 2 cut(s) 538, 825
SaqAI TTAA 1 cut(s) 1004
SatI GCNGC 2 cut(s) 99, 504
Sau3AI GATC 6 cut(s) 163, 331, 397, 439, 619, 778
SchI GAGTC 1 cut(s) 279
ScrFI CCNGG 2 cut(s) 168, 840
SfcI CTRYAG 3 cut(s) 156, 192, 510
SfuI TTCGAA 1 cut(s) 684
Sse9I AATT 7 cut(s) 41, 54, 205, 460, 931, 950, 997
SsiI CCGC 1 cut(s) 98
SspI AATATT 1 cut(s) 805
SspMI CTAG 1 cut(s) 1012
StyD4I CCNGG 2 cut(s) 166, 838
TaaI ACNGT 1 cut(s) 481
TaqI TCGA 2 cut(s) 518, 684
TasI AATT 7 cut(s) 41, 54, 205, 460, 931, 950, 997
TatI WGTACW 2 cut(s) 537, 824
TauI GCSGC 1 cut(s) 101
TfiI GAWTC 5 cut(s) 152, 293, 346, 632, 854
Tru1I TTAA 1 cut(s) 1004
Tru9I TTAA 1 cut(s) 1004
TscAI CASTG 1 cut(s) 141
TseFI GTSAC 1 cut(s) 548
TseI GCWGC 1 cut(s) 503
Tsp45I GTSAC 1 cut(s) 548
TspDTI ATGAA 4 cut(s) 17, 338, 846, 921
TspRI CASTG 1 cut(s) 141
XapI RAATTY 3 cut(s) 41, 54, 950
XceI RCATGY 1 cut(s) 454
XmiI GTMKAC 1 cut(s) 708
XmnI GAANNNNTTC 1 cut(s) 126
XspI CTAG 1 cut(s) 1012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.