pycom15g21330

GDSL esterase lipase EXL3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0028417
Physical Location & Seq
Reverse (-)
15331139 .. 15333252
2114 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g21330.2

Sequence Viewer

Length: 972 bp
ATGAAGTTTCTCTCCCAAAGCCTTCTTCCCTCGTCATCGAAATTTTCTCTCAAAATTTTTCCGATCGTTATAGTTTTATTCTTCTACAACAATGTTGCGGCTGTAAAACTACCAAACAATGAAAAGGTTCCGGCAGTGATTGTTTTCGGAGATTCAGTAGTGGATCCTGGAAACAACAACAATATTAGCACTATAGTCAAATGTAATTTCCCACCATATGGGAGAGACTTTGTAGGACAAAGACCTACCGGAAGGTTTAGCAATGGCAGAAAGATATTGCCAGCTTATCTGGATCCAAATCTGAAGATTCACGACTTACTTACTGGTGTAAGTTTTGCCTCAGGTGGCTCAGGATATGATCCTCTCACTCCTAAAATAGTGGTAAATACAAACATATACATAGACATATACATCGATGTATGTATGTCTATATATATATATATATATATATATATATATATAATATTTAGGTCTGTCCTATCATTATCAGATCAAATACACATGTTCAAAAATTATATAAGCAAGATTACGGTAGCAGTCGGAAAGGAGGGCGCTGCAACTATAGTATCGAAAAGCATATACATTGTGTGCACAGGAAGCGACGACATTGCGCATACCTATTATTTTACACCACTGAGGAGACCTCGGTATGACATTTCAGCATATACCGATCTCATGGTTGAATCAGCTTCAAGTTTCTTTCAGTCTCGAAAACCAAATGCCACAGTGCATATTAATCATAATGTTGATGTATACAACGATTTATATGTGGCTTCAGGATTTGAAGTAATGGATCAAGGATGTTGTGGAACAGGAAATATTGAGGTTAGCATTCTGTGTACTCGTTACTCTCCGGGAACCTGCAACGATTCATCAAAATACATATTCTGGGACAGCTACCATCCTTCAGAGAAGGGATATGAAGTAATAATCCCTCTGGTTTTCGATTCGCAAGTTCATAAATTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

324

Amino Acids

36.24

Weight (kDa)

7.57

Isoelectric Point (pI)

37.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028417)

Species Orthologous Gene IDs
malus_domestica MD15G1241800.v1.1
pyrus_communis pycom15g21330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 612
Acc36I ACCTGC 1 cut(s) 869
AccB7I CCANNNNNTGG 1 cut(s) 218
AccI GTMKAC 1 cut(s) 753
AciI CCGC 1 cut(s) 98
AclWI GGATC 6 cut(s) 158, 171, 287, 300, 353, 801
AcsI RAATTY 3 cut(s) 41, 54, 962
AcuI CTGAAG 3 cut(s) 323, 759, 891
AfaI GTAC 1 cut(s) 841
AfiI CCNNNNNNNGG 1 cut(s) 218
AflIII ACRYGT 1 cut(s) 501
AgsI TTSAA 4 cut(s) 508, 683, 693, 785
AjnI CCWGG 1 cut(s) 166
AjuI GAANNNNNNNTTGG 2 cut(s) 9, 41
AluBI AGCT 3 cut(s) 284, 689, 897
AluI AGCT 3 cut(s) 284, 689, 897
Alw21I GWGCWC 1 cut(s) 593
Alw26I GTCTC 3 cut(s) 219, 634, 711
Alw44I GTGCAC 1 cut(s) 589
AlwI GGATC 6 cut(s) 158, 171, 287, 300, 353, 801
ApaLI GTGCAC 1 cut(s) 589
ApeKI GCWGC 1 cut(s) 554
ApoI RAATTY 3 cut(s) 41, 54, 962
AseI ATTAAT 1 cut(s) 735
Asp700I GAANNNNTTC 1 cut(s) 126
AspLEI GCGC 2 cut(s) 554, 613
AsuC2I CCSGG 1 cut(s) 855
AxyI CCTNAGG 1 cut(s) 340
BaeGI GKGCMC 1 cut(s) 593
BaeI ACNNNNGTAYC 2 cut(s) 549, 582
BamHI GGATCC 2 cut(s) 163, 292
Bbv12I GWGCWC 1 cut(s) 593
BbvI GCAGC 1 cut(s) 541
BccI CCATC 1 cut(s) 909
BcgI CGANNNNNNTGC 2 cut(s) 590, 624
BciT130I CCWGG 1 cut(s) 168
BcnI CCSGG 1 cut(s) 855
BcoDI GTCTC 3 cut(s) 219, 634, 711
BfmI CTRYAG 2 cut(s) 192, 561
BfoI RGCGCY 1 cut(s) 555
BfuAI ACCTGC 1 cut(s) 869
BisI GCNGC 2 cut(s) 99, 555
BlsI GCNGC 2 cut(s) 100, 556
Bme1390I CCNGG 2 cut(s) 168, 855
BmiI GGNNCC 4 cut(s) 129, 165, 294, 859
BmrFI CCNGG 2 cut(s) 168, 855
BplI GAGNNNNNCTC 2 cut(s) 628, 660
Bpu10I CCTNAGC 1 cut(s) 349
BpuMI CCSGG 1 cut(s) 855
Bsa29I ATCGAT 1 cut(s) 414
BsaBI GATNNNNATC 1 cut(s) 297
BsaI GGTCTC 1 cut(s) 634
BsaJI CCNNGG 1 cut(s) 644
BsaWI WCCGGW 1 cut(s) 248
BsaXI ACNNNNNCTCC 2 cut(s) 141, 171
Bsc4I CCNNNNNNNGG 1 cut(s) 218
Bse1I ACTGG 1 cut(s) 328
Bse21I CCTNAGG 1 cut(s) 340
Bse3DI GCAATG 2 cut(s) 268, 606
Bse8I GATNNNNATC 1 cut(s) 297
BseBI CCWGG 1 cut(s) 168
BseCI ATCGAT 1 cut(s) 414
BseDI CCNNGG 1 cut(s) 644
BseGI GGATG 2 cut(s) 806, 901
BseJI GATNNNNATC 1 cut(s) 297
BseLI CCNNNNNNNGG 1 cut(s) 218
BseMI GCAATG 2 cut(s) 268, 606
BseMII CTCAG 3 cut(s) 354, 363, 626
BseNI ACTGG 1 cut(s) 328
BseRI GAGGAG 1 cut(s) 652
BseSI GKGCMC 1 cut(s) 593
BseXI GCAGC 1 cut(s) 541
Bsh1285I CGRYCG 1 cut(s) 66
BshVI ATCGAT 1 cut(s) 414
BsiEI CGRYCG 1 cut(s) 66
BsiHKAI GWGCWC 1 cut(s) 593
BsiSI CCGG 3 cut(s) 131, 249, 854
BslFI GGGAC 1 cut(s) 905
BslI CCNNNNNNNGG 1 cut(s) 218
BsmAI GTCTC 3 cut(s) 219, 634, 711
BsmFI GGGAC 1 cut(s) 905
BsmI GAATGC 1 cut(s) 831
Bso31I GGTCTC 1 cut(s) 634
Bsp1286I GDGCHC 1 cut(s) 593
Bsp143I GATC 7 cut(s) 63, 163, 292, 358, 490, 670, 793
BspACI CCGC 1 cut(s) 98
BspCNI CTCAG 3 cut(s) 353, 362, 627
BspDI ATCGAT 1 cut(s) 414
BspLI GGNNCC 4 cut(s) 129, 165, 294, 859
BspMI ACCTGC 1 cut(s) 869
BspPI GGATC 6 cut(s) 158, 171, 287, 300, 353, 801
BspTNI GGTCTC 1 cut(s) 634
BsrDI GCAATG 2 cut(s) 268, 606
BsrI ACTGG 1 cut(s) 328
BssECI CCNNGG 1 cut(s) 644
BssMI GATC 7 cut(s) 63, 163, 292, 358, 490, 670, 793
BssNAI GTATAC 1 cut(s) 754
Bst1107I GTATAC 1 cut(s) 754
Bst2UI CCWGG 1 cut(s) 168
Bst4CI ACNGT 2 cut(s) 532, 727
BstC8I GCNNGC 1 cut(s) 282
BstDEI CTNAG 3 cut(s) 340, 349, 635
BstF5I GGATG 2 cut(s) 806, 901
BstH2I RGCGCY 1 cut(s) 555
BstHHI GCGC 2 cut(s) 554, 613
BstKTI GATC 7 cut(s) 66, 166, 295, 361, 493, 673, 796
BstMAI GTCTC 3 cut(s) 219, 634, 711
BstMBI GATC 7 cut(s) 63, 163, 292, 358, 490, 670, 793
BstMCI CGRYCG 1 cut(s) 66
BstMWI GCNNNNNNNGC 1 cut(s) 597
BstNI CCWGG 1 cut(s) 168
BstNSI RCATGY 1 cut(s) 505
BstSCI CCNGG 2 cut(s) 166, 853
BstSFI CTRYAG 2 cut(s) 192, 561
BstSLI GKGCMC 1 cut(s) 593
BstV1I GCAGC 1 cut(s) 541
BstX2I RGATCY 2 cut(s) 163, 292
BstYI RGATCY 2 cut(s) 163, 292
BstZ17I GTATAC 1 cut(s) 754
Bsu15I ATCGAT 1 cut(s) 414
Bsu36I CCTNAGG 1 cut(s) 340
BsuTUI ATCGAT 1 cut(s) 414
BtsCI GGATG 2 cut(s) 806, 901
BtsI GCAGTG 1 cut(s) 141
BtsIMutI CAGTG 3 cut(s) 141, 632, 732
BveI ACCTGC 1 cut(s) 869
Cac8I GCNNGC 1 cut(s) 282
CfoI GCGC 2 cut(s) 554, 613
ClaI ATCGAT 1 cut(s) 414
Csp6I GTAC 1 cut(s) 840
CviAII CATG 2 cut(s) 502, 676
CviJI RGCY 7 cut(s) 21, 101, 284, 348, 689, 773, 897
CviKI_1 RGCY 7 cut(s) 21, 101, 284, 348, 689, 773, 897
CviQI GTAC 1 cut(s) 840
DdeI CTNAG 3 cut(s) 340, 349, 635
DpnI GATC 7 cut(s) 65, 165, 294, 360, 492, 672, 795
DpnII GATC 7 cut(s) 63, 163, 292, 358, 490, 670, 793
Eco31I GGTCTC 1 cut(s) 634
Eco57I CTGAAG 3 cut(s) 323, 759, 891
Eco81I CCTNAGG 1 cut(s) 340
EcoRII CCWGG 1 cut(s) 166
FaeI CATG 2 cut(s) 505, 679
FaqI GGGAC 1 cut(s) 905
FatI CATG 2 cut(s) 501, 675
FauNDI CATATG 1 cut(s) 217
FblI GTMKAC 1 cut(s) 753
Fnu4HI GCNGC 2 cut(s) 99, 555
FokI GGATG 2 cut(s) 813, 888
Fsp4HI GCNGC 2 cut(s) 99, 555
FspI TGCGCA 1 cut(s) 612
GlaI GCGC 2 cut(s) 553, 612
GluI GCNGC 2 cut(s) 99, 555
HaeII RGCGCY 1 cut(s) 555
HapII CCGG 3 cut(s) 131, 249, 854
HhaI GCGC 2 cut(s) 554, 613
Hin1II CATG 2 cut(s) 505, 679
Hin6I GCGC 2 cut(s) 552, 611
HinP1I GCGC 2 cut(s) 552, 611
HinfI GANTC 5 cut(s) 152, 307, 683, 869, 947
HpaII CCGG 3 cut(s) 131, 249, 854
Hpy166II GTNNAC 3 cut(s) 591, 754, 840
Hpy188I TCNGA 7 cut(s) 63, 149, 303, 490, 542, 910, 971
Hpy188III TCNNGA 5 cut(s) 290, 311, 351, 708, 777
Hpy8I GTNNAC 3 cut(s) 591, 754, 840
Hpy99I CGWCG 1 cut(s) 605
HpyAV CCTTC 4 cut(s) 32, 246, 907, 915
HpyCH4III ACNGT 2 cut(s) 532, 727
HpyCH4V TGCA 4 cut(s) 557, 591, 730, 864
HpyF10VI GCNNNNNNNGC 1 cut(s) 597
HpyF3I CTNAG 3 cut(s) 340, 349, 635
Hsp92II CATG 2 cut(s) 505, 679
HspAI GCGC 2 cut(s) 552, 611
Kzo9I GATC 7 cut(s) 63, 163, 292, 358, 490, 670, 793
Lsp1109I GCAGC 1 cut(s) 541
MaeIII GTNAC 1 cut(s) 845
MalI GATC 7 cut(s) 65, 165, 294, 360, 492, 672, 795
MboI GATC 7 cut(s) 63, 163, 292, 358, 490, 670, 793
MboII GAAGA 4 cut(s) 17, 73, 316, 958
MflI RGATCY 2 cut(s) 163, 292
MhlI GDGCHC 1 cut(s) 593
MluCI AATT 5 cut(s) 41, 54, 205, 511, 962
MmeI TCCRAC 1 cut(s) 520
MnlI CCTC 8 cut(s) 40, 349, 372, 541, 630, 654, 817, 945
MroXI GAANNNNTTC 1 cut(s) 126
MseI TTAA 1 cut(s) 735
MspI CCGG 3 cut(s) 131, 249, 854
MspR9I CCNGG 2 cut(s) 168, 855
Mva1269I GAATGC 1 cut(s) 831
MvaI CCWGG 1 cut(s) 168
MwoI GCNNNNNNNGC 1 cut(s) 597
NciI CCSGG 1 cut(s) 855
NdeI CATATG 1 cut(s) 217
NdeII GATC 7 cut(s) 63, 163, 292, 358, 490, 670, 793
NlaIII CATG 2 cut(s) 505, 679
NlaIV GGNNCC 4 cut(s) 129, 165, 294, 859
NsbI TGCGCA 1 cut(s) 612
NspI RCATGY 1 cut(s) 505
PciI ACATGT 1 cut(s) 501
PctI GAATGC 1 cut(s) 831
PdmI GAANNNNTTC 1 cut(s) 126
PfeI GAWTC 5 cut(s) 152, 307, 683, 869, 947
PflMI CCANNNNNTGG 1 cut(s) 218
PfoI TCCNGGA 2 cut(s) 166, 853
PkrI GCNGC 2 cut(s) 100, 556
Ple19I CGATCG 1 cut(s) 66
PscI ACATGT 1 cut(s) 501
PshBI ATTAAT 1 cut(s) 735
Psp6I CCWGG 1 cut(s) 166
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 4 cut(s) 129, 165, 294, 859
PsuI RGATCY 2 cut(s) 163, 292
PvuI CGATCG 1 cut(s) 66
RsaI GTAC 1 cut(s) 841
RsaNI GTAC 1 cut(s) 840
SaqAI TTAA 1 cut(s) 735
SatI GCNGC 2 cut(s) 99, 555
Sau3AI GATC 7 cut(s) 63, 163, 292, 358, 490, 670, 793
ScrFI CCNGG 2 cut(s) 168, 855
SduI GDGCHC 1 cut(s) 593
SfcI CTRYAG 2 cut(s) 192, 561
Sse9I AATT 5 cut(s) 41, 54, 205, 511, 962
SsiI CCGC 1 cut(s) 98
SspI AATATT 3 cut(s) 184, 465, 820
StyD4I CCNGG 2 cut(s) 166, 853
TaaI ACNGT 2 cut(s) 532, 727
TaqI TCGA 5 cut(s) 38, 414, 569, 709, 945
TasI AATT 5 cut(s) 41, 54, 205, 511, 962
TatI WGTACW 1 cut(s) 839
TauI GCSGC 1 cut(s) 101
TfiI GAWTC 5 cut(s) 152, 307, 683, 869, 947
Tru1I TTAA 1 cut(s) 735
Tru9I TTAA 1 cut(s) 735
TscAI CASTG 3 cut(s) 141, 639, 732
TseI GCWGC 1 cut(s) 554
TspDTI ATGAA 5 cut(s) 17, 135, 861, 936, 947
TspRI CASTG 3 cut(s) 141, 639, 732
Van91I CCANNNNNTGG 1 cut(s) 218
VneI GTGCAC 1 cut(s) 589
VspI ATTAAT 1 cut(s) 735
XapI RAATTY 3 cut(s) 41, 54, 962
XceI RCATGY 1 cut(s) 505
XmiI GTMKAC 1 cut(s) 753
XmnI GAANNNNTTC 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.