MD15G1325200.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005305
Physical Location & Seq
Reverse (-)
34688373 .. 34694863
6491 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1325200.v1.1.491

Sequence Viewer

Length: 1923 bp
ATGGATAAAGTGTCTTCTGACTGTCCATACCCAGGATGTTTCTTTTGTGTCATGAAGGAAGGGAACCCAAGTAAGCGAAGAGCCAGCATCCTGAAGTTCTTTAGAGAGCTTCCTGCACAGGATGACGATGGCCAGGTTCTTCCTATCAGTGGCCTTTGGAACACTGCCATGGCACATCCGAATGACCCTGAGTTCATCGAGCTTGGCATCTTTGAATGTATGGCTGCATTGATATGGAAGGGTCTAAAGAACCGTCGCTGGCTTTCTCATGATCAGAATATATACATTCCTTATTATGCTGCTCATATTATTGGATCATACACCATGAACATGGAAGAATTTGCTGCAAGTGCTGTTCATGCTGGGGTAATCCCTCCCTTAGTTGAGCTTTTGAGAGGAAGGTTAACTTGGGTTGAACAGAGAGTGGCAGTTCGAGCTTTAGGGCACTTGGCTACCTATGCCAGCACTTTTCCTGCCGTAGCAAGTCATGGCGAAATTCTTGAGCTTTCGATTCAACTAGCAATGAGCTCACTGGAAATAGTCTATTCTCATTTTTACCAGTATGTTGACAGAAGGTTAAGCTATCACTGCGATCTGCTCACCCGCGGTATGGGAGGTGTTGAAATGGAGTCCAGAAAGGCAGAAGAGTGGGCGAGTCAGTTACAGTGCTGGTCCCTCCAGCTTATTAACTGCTTTGCCTTTAAACCGGAGTTTCTTCCTACCATATGCAATCCTGAATTCTTAACGAAACTACCTGGCATGTGGGGTGGGCTTGTTAATGAAAACTCACCAGCTGGTATCGGTTTATTAAGGACAATTTGCCATCATAAGCTCGGTAGAGGACCAGTTGCTGGCTGTCCCAGTATCATCGAATCTTTGTGTAATATATCTCGGTCATCAGATGATTGGCAGTATATGACTATTGATTGCCTTCTGTGGTTGCTTCAAGACCCTAGTACCTGCCATAAGGTTGTTGATAAGGCCGTACCTGCACTTGTTGACCTTGCTGAGATTACAACTCTGGGTGATCACAAGAAACTAGGAGATTCCATTGTTAATGTTCTTGAGGAATGTGTCCAGTCCCAAGGGACAGGACGTAACTCTTTTAGTAACCGAACGAAAGAATTGATCGAGGAACTTTTGAATTCCAGACAGAGATTGAAATGGGAAAAGAATATGCCTAAAGAGGATCTCCATATTAAACAGGCTGCAGCACTAGTGGTCAAGCTTGAAGGAAATTCTCTGTTCTCATCAGGAAATATATCTGGAGCTGCATCAAAATACTCAGAAGCTCTGGCATTGTGTCCGATGAGATCCAAAAAGGAGAGGGTTGTTCTGTACAGTAACCGAGCTCAGTGTCATCTTCTGTTGCAACAGCCCTTGACAGCTATTAGTGATGCTACTCGTGCACTTTGTCTTCACAACCCACTGAACCGTCATGCCAAAAGTCTTTGGAGAAGAGCGCAGGCTTATGACATGCTTGGGTTTGCAAAAGAGAGTTTGCTAGATGCCATTCTGTTCATAAACGAGTGCTCTCAGTCAAATGATCCTGATCTCTCATTGAGGCAGAATAAAGTTCCAGACTATGCTGAACGATTGGTTAAAAAGCAGATGCGTGCAGCGTGGTTATTTAGAGAAGCAGCTATCAAACATGGGGGTGTCCATTGTGAGGGTGATGCTGGTGACGTCTTTGGCCGAGAGACTGATGATTCCGAATGGGAGACTGCAAGCGAGAGTGATATAGGAAATGATGGAAGAGATGAAATGGGAAATGAAGAGGAGGAGGAGGAGGATGATGATGATGATGATGATGATGATAGCGAATGGAAGAATAATGATGAGAGGAAAGAGAAATATGATAAGTCTTCAATTAAAGACCTAAAGCATGGATACAATGTGCAGCTTGCGGAAGATGAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

641

Amino Acids

72.05

Weight (kDa)

5.2

Isoelectric Point (pI)

55.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_7 PF26524 7 - 244 1.6e-116 Plant ARM repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1689
Acc36I ACCTGC 2 cut(s) 968, 997
AccB7I CCANNNNNTGG 1 cut(s) 851
AccII CGCG 1 cut(s) 606
AciI CCGC 3 cut(s) 604, 606, 1907
AclWI GGATC 4 cut(s) 322, 1197, 1308, 1541
AcoI YGGCCR 2 cut(s) 130, 1693
AcsI RAATTY 5 cut(s) 338, 495, 737, 1144, 1237
AcuI CTGAAG 1 cut(s) 113
AcyI GRCGYC 1 cut(s) 1686
AfaI GTAC 3 cut(s) 958, 987, 1340
AfiI CCNNNNNNNGG 5 cut(s) 32, 149, 610, 851, 1669
AgsI TTSAA 9 cut(s) 215, 416, 515, 623, 947, 1144, 1162, 1232, 1869
AhlI ACTAGT 1 cut(s) 1216
AjnI CCWGG 3 cut(s) 31, 132, 754
AjuI GAANNNNNNNTTGG 2 cut(s) 391, 423
Alw21I GWGCWC 4 cut(s) 530, 1354, 1411, 1535
Alw26I GTCTC 2 cut(s) 1694, 1715
Alw44I GTGCAC 1 cut(s) 1407
AlwI GGATC 4 cut(s) 322, 1197, 1308, 1541
AlwNI CAGNNNCTG 1 cut(s) 851
AoxI GGCC 4 cut(s) 130, 151, 981, 1693
ApaLI GTGCAC 1 cut(s) 1407
ApeKI GCWGC 9 cut(s) 224, 299, 344, 1208, 1211, 1271, 1619, 1640, 1900
ApoI RAATTY 5 cut(s) 338, 495, 737, 1144, 1237
AspLEI GCGC 1 cut(s) 1465
AspS9I GGNCC 2 cut(s) 672, 842
AsuHPI GGTGA 5 cut(s) 592, 780, 1037, 1685, 1694
AvaII GGWCC 2 cut(s) 672, 842
BaeGI GKGCMC 2 cut(s) 447, 1411
BaeI ACNNNNGTAYC 2 cut(s) 940, 973
BalI TGGCCA 1 cut(s) 132
BanII GRGCYC 2 cut(s) 530, 1354
BauI CACGAG 1 cut(s) 1404
BbsI GAAGAC 3 cut(s) 6, 1409, 1857
Bbv12I GWGCWC 4 cut(s) 530, 1354, 1411, 1535
BbvI GCAGC 9 cut(s) 211, 286, 331, 1195, 1223, 1258, 1631, 1652, 1912
BccI CCATC 3 cut(s) 122, 831, 1745
BceAI ACGGC 2 cut(s) 461, 968
BciT130I CCWGG 3 cut(s) 33, 134, 756
BciVI GTATCC 1 cut(s) 1883
BclI TGATCA 2 cut(s) 271, 1027
BcoDI GTCTC 2 cut(s) 1694, 1715
BcuI ACTAGT 1 cut(s) 1216
BfaI CTAG 5 cut(s) 518, 954, 1040, 1217, 1505
BfmI CTRYAG 1 cut(s) 1209
BfuAI ACCTGC 2 cut(s) 968, 997
BfuI GTATCC 1 cut(s) 1883
BisI GCNGC 9 cut(s) 225, 300, 345, 1209, 1212, 1272, 1620, 1641, 1901
BlsI GCNGC 9 cut(s) 226, 301, 346, 1210, 1213, 1273, 1621, 1642, 1902
Bme1390I CCNGG 3 cut(s) 33, 134, 756
Bme18I GGWCC 2 cut(s) 672, 842
BmgT120I GGNCC 2 cut(s) 672, 842
BmiI GGNNCC 2 cut(s) 65, 674
BmrFI CCNGG 3 cut(s) 33, 134, 756
BmrI ACTGGG 1 cut(s) 855
BmsI GCATC 7 cut(s) 96, 216, 1283, 1387, 1498, 1602, 1666
BmuI ACTGGG 1 cut(s) 855
BpiI GAAGAC 3 cut(s) 6, 1409, 1857
BpmI CTGGAG 2 cut(s) 662, 1287
BpuEI CTTGAG 2 cut(s) 521, 1085
BsaBI GATNNNNATC 1 cut(s) 1551
BsaHI GRCGYC 1 cut(s) 1686
BsaJI CCNNGG 4 cut(s) 31, 168, 604, 1084
BsaWI WCCGGW 1 cut(s) 706
Bsc4I CCNNNNNNNGG 5 cut(s) 32, 149, 610, 851, 1669
Bse1I ACTGG 5 cut(s) 537, 559, 845, 861, 1078
Bse3DI GCAATG 1 cut(s) 528
Bse8I GATNNNNATC 1 cut(s) 1551
BseBI CCWGG 3 cut(s) 33, 134, 756
BseDI CCNNGG 4 cut(s) 31, 168, 604, 1084
BseGI GGATG 5 cut(s) 41, 87, 127, 175, 1798
BseJI GATNNNNATC 1 cut(s) 1551
BseLI CCNNNNNNNGG 5 cut(s) 32, 149, 610, 851, 1669
BseMI GCAATG 1 cut(s) 528
BseMII CTCAG 5 cut(s) 180, 999, 1299, 1367, 1550
BseNI ACTGG 5 cut(s) 537, 559, 845, 861, 1078
BseRI GAGGAG 4 cut(s) 1793, 1796, 1799, 1802
BseSI GKGCMC 2 cut(s) 447, 1411
BseXI GCAGC 9 cut(s) 211, 286, 331, 1195, 1223, 1258, 1631, 1652, 1912
BseYI CCCAGC 1 cut(s) 362
BsgI GTGCAG 4 cut(s) 99, 975, 1638, 1919
Bsh1236I CGCG 1 cut(s) 606
BshFI GGCC 4 cut(s) 132, 153, 983, 1695
BsiHKAI GWGCWC 4 cut(s) 530, 1354, 1411, 1535
BsiSI CCGG 1 cut(s) 707
BslFI GGGAC 4 cut(s) 658, 843, 1066, 1102
BslI CCNNNNNNNGG 5 cut(s) 32, 149, 610, 851, 1669
BsmAI GTCTC 2 cut(s) 1694, 1715
BsmFI GGGAC 4 cut(s) 658, 843, 1066, 1102
BsnI GGCC 4 cut(s) 132, 153, 983, 1695
Bsp1286I GDGCHC 5 cut(s) 447, 530, 1354, 1411, 1535
Bsp1407I TGTACA 1 cut(s) 1338
Bsp143I GATC 9 cut(s) 271, 314, 592, 1027, 1128, 1189, 1313, 1546, 1552
Bsp19I CCATGG 1 cut(s) 168
BspACI CCGC 3 cut(s) 604, 606, 1907
BspANI GGCC 4 cut(s) 132, 153, 983, 1695
BspCNI CTCAG 5 cut(s) 181, 1000, 1298, 1366, 1549
BspFNI CGCG 1 cut(s) 606
BspHI TCATGA 2 cut(s) 51, 268
BspLI GGNNCC 2 cut(s) 65, 674
BspMAI CTGCAG 1 cut(s) 1213
BspMI ACCTGC 2 cut(s) 968, 997
BspPI GGATC 4 cut(s) 322, 1197, 1308, 1541
BspQI GCTCTTC 2 cut(s) 73, 1453
BsrDI GCAATG 1 cut(s) 528
BsrGI TGTACA 1 cut(s) 1338
BsrI ACTGG 5 cut(s) 537, 559, 845, 861, 1078
BssECI CCNNGG 4 cut(s) 31, 168, 604, 1084
BssMI GATC 9 cut(s) 271, 314, 592, 1027, 1128, 1189, 1313, 1546, 1552
BssNI GRCGYC 1 cut(s) 1686
BssSI CACGAG 1 cut(s) 1404
BssT1I CCWWGG 2 cut(s) 168, 1084
Bst2BI CACGAG 1 cut(s) 1404
Bst2UI CCWGG 3 cut(s) 33, 134, 756
Bst4CI ACNGT 5 cut(s) 23, 254, 666, 1343, 1436
Bst6I CTCTTC 5 cut(s) 73, 639, 1453, 1750, 1770
BstACI GRCGYC 1 cut(s) 1686
BstAUI TGTACA 1 cut(s) 1338
BstC8I GCNNGC 8 cut(s) 85, 260, 463, 853, 1467, 1617, 1729, 1905
BstDEI CTNAG 6 cut(s) 189, 379, 1008, 1285, 1353, 1536
BstDSI CCRYGG 2 cut(s) 168, 604
BstF5I GGATG 5 cut(s) 41, 87, 127, 175, 1798
BstFNI CGCG 1 cut(s) 606
BstHHI GCGC 1 cut(s) 1465
BstKTI GATC 9 cut(s) 274, 317, 595, 1030, 1131, 1192, 1316, 1549, 1555
BstMAI GTCTC 2 cut(s) 1694, 1715
BstMBI GATC 9 cut(s) 271, 314, 592, 1027, 1128, 1189, 1313, 1546, 1552
BstMWI GCNNNNNNNGC 7 cut(s) 350, 359, 434, 458, 588, 989, 1406
BstNI CCWGG 3 cut(s) 33, 134, 756
BstNSI RCATGY 2 cut(s) 763, 1480
BstSCI CCNGG 3 cut(s) 31, 132, 754
BstSFI CTRYAG 1 cut(s) 1209
BstSLI GKGCMC 2 cut(s) 447, 1411
BstUI CGCG 1 cut(s) 606
BstV1I GCAGC 9 cut(s) 211, 286, 331, 1195, 1223, 1258, 1631, 1652, 1912
BstV2I GAAGAC 3 cut(s) 6, 1409, 1857
BstX2I RGATCY 2 cut(s) 1189, 1313
BstXI CCANNNNNNTGG 1 cut(s) 331
BstYI RGATCY 2 cut(s) 1189, 1313
BsuI GTATCC 1 cut(s) 1883
BsuRI GGCC 4 cut(s) 132, 153, 983, 1695
BtgI CCRYGG 2 cut(s) 168, 604
BtsCI GGATG 5 cut(s) 41, 87, 127, 175, 1798
BtsI GCAGTG 2 cut(s) 162, 586
BtsIMutI CAGTG 7 cut(s) 154, 162, 530, 586, 671, 1361, 1427
BveI ACCTGC 2 cut(s) 968, 997
Cac8I GCNNGC 8 cut(s) 85, 260, 463, 853, 1467, 1617, 1729, 1905
CaiI CAGNNNCTG 1 cut(s) 851
CciI TCATGA 2 cut(s) 51, 268
CfoI GCGC 1 cut(s) 1465
Cfr13I GGNCC 2 cut(s) 672, 842
Cfr42I CCGCGG 1 cut(s) 607
Csp6I GTAC 3 cut(s) 957, 986, 1339
CviQI GTAC 3 cut(s) 957, 986, 1339
DdeI CTNAG 6 cut(s) 189, 379, 1008, 1285, 1353, 1536
DpnI GATC 9 cut(s) 273, 316, 594, 1029, 1130, 1191, 1315, 1548, 1554
DpnII GATC 9 cut(s) 271, 314, 592, 1027, 1128, 1189, 1313, 1546, 1552
DraI TTTAAA 1 cut(s) 703
EaeI YGGCCR 2 cut(s) 130, 1693
Eam1104I CTCTTC 5 cut(s) 73, 639, 1453, 1750, 1770
EarI CTCTTC 5 cut(s) 73, 639, 1453, 1750, 1770
Ecl136II GAGCTC 2 cut(s) 528, 1352
Eco130I CCWWGG 2 cut(s) 168, 1084
Eco24I GRGCYC 2 cut(s) 530, 1354
Eco47I GGWCC 2 cut(s) 672, 842
Eco53kI GAGCTC 2 cut(s) 528, 1352
Eco57I CTGAAG 1 cut(s) 113
EcoICRI GAGCTC 2 cut(s) 528, 1352
EcoRI GAATTC 2 cut(s) 737, 1144
EcoRII CCWGG 3 cut(s) 31, 132, 754
EcoT14I CCWWGG 2 cut(s) 168, 1084
EcoT38I GRGCYC 2 cut(s) 530, 1354
ErhI CCWWGG 2 cut(s) 168, 1084
FalI AAGNNNNNCTT 2 cut(s) 391, 423
FaqI GGGAC 4 cut(s) 658, 843, 1066, 1102
FauI CCCGC 1 cut(s) 611
FauNDI CATATG 1 cut(s) 725
FbaI TGATCA 2 cut(s) 271, 1027
Fnu4HI GCNGC 9 cut(s) 225, 300, 345, 1209, 1212, 1272, 1620, 1641, 1901
FokI GGATG 5 cut(s) 48, 74, 134, 162, 1805
FriOI GRGCYC 2 cut(s) 530, 1354
Fsp4HI GCNGC 9 cut(s) 225, 300, 345, 1209, 1212, 1272, 1620, 1641, 1901
FspBI CTAG 5 cut(s) 518, 954, 1040, 1217, 1505
GlaI GCGC 1 cut(s) 1464
GluI GCNGC 9 cut(s) 225, 300, 345, 1209, 1212, 1272, 1620, 1641, 1901
GsaI CCCAGC 1 cut(s) 366
GsuI CTGGAG 2 cut(s) 662, 1287
HaeIII GGCC 4 cut(s) 132, 153, 983, 1695
HapII CCGG 1 cut(s) 707
HhaI GCGC 1 cut(s) 1465
Hin1I GRCGYC 1 cut(s) 1686
Hin6I GCGC 1 cut(s) 1463
HinP1I GCGC 1 cut(s) 1463
HincII GTYRAC 3 cut(s) 405, 568, 1000
HindII GTYRAC 3 cut(s) 405, 568, 1000
HindIII AAGCTT 1 cut(s) 1226
HinfI GANTC 6 cut(s) 511, 629, 655, 872, 1046, 1709
HpaI GTTAAC 1 cut(s) 405
HpaII CCGG 1 cut(s) 707
HphI GGTGA 5 cut(s) 592, 780, 1037, 1685, 1694
Hpy166II GTNNAC 4 cut(s) 405, 568, 1000, 1409
Hpy188I TCNGA 7 cut(s) 19, 180, 276, 901, 1288, 1308, 1714
Hpy8I GTNNAC 4 cut(s) 405, 568, 1000, 1409
Hpy99I CGWCG 1 cut(s) 258
HpyAV CCTTC 7 cut(s) 49, 53, 232, 393, 567, 941, 1226
HpyCH4III ACNGT 5 cut(s) 23, 254, 666, 1343, 1436
HpyCH4IV ACGT 2 cut(s) 1096, 1686
HpyF10VI GCNNNNNNNGC 7 cut(s) 350, 359, 434, 458, 588, 989, 1406
HpyF3I CTNAG 6 cut(s) 189, 379, 1008, 1285, 1353, 1536
HpySE526I ACGT 2 cut(s) 1096, 1686
Hsp92I GRCGYC 1 cut(s) 1686
HspAI GCGC 1 cut(s) 1463
Ksp22I TGATCA 2 cut(s) 271, 1027
KspAI GTTAAC 1 cut(s) 405
KspI CCGCGG 1 cut(s) 607
Kzo9I GATC 9 cut(s) 271, 314, 592, 1027, 1128, 1189, 1313, 1546, 1552
LguI GCTCTTC 2 cut(s) 73, 1453
LmnI GCTCC 1 cut(s) 1268
Lsp1109I GCAGC 9 cut(s) 211, 286, 331, 1195, 1223, 1258, 1631, 1652, 1912
LweI GCATC 7 cut(s) 96, 216, 1283, 1387, 1498, 1602, 1666
MaeI CTAG 5 cut(s) 518, 954, 1040, 1217, 1505
MaeII ACGT 2 cut(s) 1096, 1686
MaeIII GTNAC 5 cut(s) 660, 1097, 1109, 1343, 1682
MalI GATC 9 cut(s) 273, 316, 594, 1029, 1130, 1191, 1315, 1548, 1554
MboI GATC 9 cut(s) 271, 314, 592, 1027, 1128, 1189, 1313, 1546, 1552
MflI RGATCY 2 cut(s) 1189, 1313
MhlI GDGCHC 5 cut(s) 447, 530, 1354, 1411, 1535
MlsI TGGCCA 1 cut(s) 132
MluCI AATT 8 cut(s) 338, 495, 737, 816, 1124, 1144, 1237, 1869
MluNI TGGCCA 1 cut(s) 132
MlyI GAGTC 2 cut(s) 638, 664
Mox20I TGGCCA 1 cut(s) 132
MscI TGGCCA 1 cut(s) 132
MslI CAYNNNNRTG 5 cut(s) 167, 180, 232, 329, 1656
Msp20I TGGCCA 1 cut(s) 132
MspA1I CMGCKG 2 cut(s) 606, 794
MspI CCGG 1 cut(s) 707
MspR9I CCNGG 3 cut(s) 33, 134, 756
MvaI CCWGG 3 cut(s) 33, 134, 756
MvnI CGCG 1 cut(s) 606
MwoI GCNNNNNNNGC 7 cut(s) 350, 359, 434, 458, 588, 989, 1406
NcoI CCATGG 1 cut(s) 168
NdeI CATATG 1 cut(s) 725
NdeII GATC 9 cut(s) 271, 314, 592, 1027, 1128, 1189, 1313, 1546, 1552
NlaIV GGNNCC 2 cut(s) 65, 674
NmeAIII GCCGAG 1 cut(s) 1721
NmuCI GTSAC 1 cut(s) 1682
NspI RCATGY 2 cut(s) 763, 1480
PagI TCATGA 2 cut(s) 51, 268
PciSI GCTCTTC 2 cut(s) 73, 1453
PfeI GAWTC 4 cut(s) 511, 872, 1046, 1709
PflMI CCANNNNNTGG 1 cut(s) 851
PkrI GCNGC 9 cut(s) 226, 301, 346, 1210, 1213, 1273, 1621, 1642, 1902
PleI GAGTC 2 cut(s) 637, 663
PpsI GAGTC 2 cut(s) 637, 663
Psp124BI GAGCTC 2 cut(s) 530, 1354
Psp6I CCWGG 3 cut(s) 31, 132, 754
PspFI CCCAGC 1 cut(s) 362
PspGI CCWGG 3 cut(s) 31, 132, 754
PspN4I GGNNCC 2 cut(s) 65, 674
PspPI GGNCC 2 cut(s) 672, 842
PstI CTGCAG 1 cut(s) 1213
PstNI CAGNNNCTG 1 cut(s) 851
PsuI RGATCY 2 cut(s) 1189, 1313
PvuII CAGCTG 1 cut(s) 794
RsaI GTAC 3 cut(s) 958, 987, 1340
RsaNI GTAC 3 cut(s) 957, 986, 1339
RseI CAYNNNNRTG 5 cut(s) 167, 180, 232, 329, 1656
SacI GAGCTC 2 cut(s) 530, 1354
SacII CCGCGG 1 cut(s) 607
SapI GCTCTTC 2 cut(s) 73, 1453
SatI GCNGC 9 cut(s) 225, 300, 345, 1209, 1212, 1272, 1620, 1641, 1901
Sau3AI GATC 9 cut(s) 271, 314, 592, 1027, 1128, 1189, 1313, 1546, 1552
Sau96I GGNCC 2 cut(s) 672, 842
SchI GAGTC 2 cut(s) 638, 664
ScrFI CCNGG 3 cut(s) 33, 134, 756
SduI GDGCHC 5 cut(s) 447, 530, 1354, 1411, 1535
SfaNI GCATC 7 cut(s) 96, 216, 1283, 1387, 1498, 1602, 1666
SfcI CTRYAG 1 cut(s) 1209
Sfr303I CCGCGG 1 cut(s) 607
SgrBI CCGCGG 1 cut(s) 607
SinI GGWCC 2 cut(s) 672, 842
SmiMI CAYNNNNRTG 5 cut(s) 167, 180, 232, 329, 1656
SmlI CTYRAG 2 cut(s) 500, 1064
SmoI CTYRAG 2 cut(s) 500, 1064
SpeI ACTAGT 1 cut(s) 1216
Sse9I AATT 8 cut(s) 338, 495, 737, 816, 1124, 1144, 1237, 1869
SsiI CCGC 3 cut(s) 604, 606, 1907
SspMI CTAG 5 cut(s) 518, 954, 1040, 1217, 1505
SstI GAGCTC 2 cut(s) 530, 1354
StyD4I CCNGG 3 cut(s) 31, 132, 754
StyI CCWWGG 2 cut(s) 168, 1084
TaaI ACNGT 5 cut(s) 23, 254, 666, 1343, 1436
TaiI ACGT 2 cut(s) 1099, 1689
TaqI TCGA 5 cut(s) 198, 433, 509, 870, 1131
TaqII GACCGA 1 cut(s) 882
TasI AATT 8 cut(s) 338, 495, 737, 816, 1124, 1144, 1237, 1869
TatI WGTACW 1 cut(s) 1338
TfiI GAWTC 4 cut(s) 511, 872, 1046, 1709
TscAI CASTG 7 cut(s) 154, 169, 537, 593, 671, 1361, 1434
TseFI GTSAC 1 cut(s) 1682
TseI GCWGC 9 cut(s) 224, 299, 344, 1208, 1211, 1271, 1619, 1640, 1900
Tsp45I GTSAC 1 cut(s) 1682
TspDTI ATGAA 8 cut(s) 68, 184, 341, 347, 795, 1510, 1776, 1788
TspRI CASTG 7 cut(s) 154, 169, 537, 593, 671, 1361, 1434
Van91I CCANNNNNTGG 1 cut(s) 851
VneI GTGCAC 1 cut(s) 1407
VpaK11BI GGWCC 2 cut(s) 672, 842
XapI RAATTY 5 cut(s) 338, 495, 737, 1144, 1237
XceI RCATGY 2 cut(s) 763, 1480
XspI CTAG 5 cut(s) 518, 954, 1040, 1217, 1505
ZraI GACGTC 1 cut(s) 1687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.