MD15G1374000.v1.1

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0021880
Physical Location & Seq
Reverse (-)
45667521 .. 45672845
5325 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1374000.v1.1.491

Sequence Viewer

Length: 1533 bp
ATGCAGAGGAGGAGCTGGAGAACATTGGCGACAGTGAGGAAGGTGCTAACGTGCGCCGTATGCTCAATTGCGCTAGTGGCTCTCTTCTCTTTCTCCAAAGTCCCCAACTTCTCCGACCCCGTCAAGCTCCCCACGTATTTGATTACTGTTGGGAAAACTAAAGAAGAAGAATTTGAATGCATCAACTTCATGGTTGGCGTTATTATGATTCAACGGAATAAGATGCTACAAAACACAATATCTTTGGACGAAAATGAGAAAAGAAACACAACAGAGTTGGATGATACCAGTTGGATATTGGATGACAAGCTATGGAAGATTCCACCGAGTCCCGATTTTATGCAATGTATAGAGCCAAGTCCCAACTATACACCTCCTGCGGAGTCACGGGGCTACTTTCAAGTGCGCACAAATGGTGGTCTCAATCAGATGCGTGCAGGGATATGTGACATGGTAGCTGTAGCCCGTATCATAAATGCCACTCTTTTAATTCCAGAACTTGATCAACGGTCATTTTGGAAAGACTCTAGCAACTTCTCAAATGTATTTGATGAAGATCATTTTATCAATGCTCTAGCTAATGACGTTAAAATTGTAAGAAAGCTTCCTAAGGAACTTGCTTCTGGTCCCAGAGCACTTAAGCTTTTCAGAAGCTGGGGCGGTATGTATTATTACCAAGATGAGATTGCTACTTCCAAAGCTGATGCTCGCTTAGTAAACAACAACCTACCTCTAGATATACAGAAACTTCACTGTTGTGCTTTTTATGAAGCTCCGCATTTTACACCTGAAATTGAAGCCATGGGAAAATTGTTAGTTGATCGGATGAGGTCATTTGGCCCCTATATTGCCCTTCATTTACGATTTGAGAAGGACATGCTTGCCTTTAGTGGATGCACGCAGGATTTAACTCCATCTGAAGCTGATGAACTAAGGATCATTCGAACAGAGATCCAGAGGGTATTTCCCTTAACTCCCAGGGAGACTGGAATTTTTCTCAGTACTCTTGGGTACCCACCAAACACACCTATCTACATTGCTGCTGGAAAGATGTATGACGATTCTCATATGGCTGATCTACTATCCCGTTTTCCAATACTGAAGAGCAAGGAAGCATTGGCATCAATTGAGGAGCTTGAACCTTTTACCAATCATGCGTCTCAAATGGCTGCGCTTGACTACATTGTTTCAGTTGAAAGTGATGTATTTATACCTACATACCTCGGACACATGGCGGCTGCAGTTGAAGGTCACCGTCGCTTTCTTGGACACAGGAAAACAATATCTCCTGATAGAAAAGCCCTTGTTTCGCTGTTTGACCAATTTGAGCAGGGAAGTCTTGAGGAAGGCAAAACCTTATCAAATAGAGTTGTTCAAATGCAAAGGAAACGGCAAGGCTCCCCACGGAAGAGAAGAGGTCCCCTTGCAGGACTTAGGGGGAAGGATAAGTTTCGTTCAGAAGAACCATTTTACGAGAACCCCTTACTAGATTGTTTGTGTAGAAAAGAATTCCCAGTGCAAAGAAGTCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

511

Amino Acids

58.19

Weight (kDa)

8.4

Isoelectric Point (pI)

46.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 132 - 421 5.3e-84 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021880)

Species Orthologous Gene IDs
malus_domestica MD08G1188000.v1.1 MD15G1374000.v1.1
pyrus_communis pycom08g16140
rosa_chinensis RchiOBHm_Chr7g0242491

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 407
Acc65I GGTACC 1 cut(s) 1011
AccB1I GGYRCC 1 cut(s) 1011
AciI CCGC 4 cut(s) 380, 660, 776, 1235
AclWI GGATC 2 cut(s) 944, 946
AcsI RAATTY 3 cut(s) 170, 990, 1508
AcuI CTGAAG 2 cut(s) 939, 1121
AfaI GTAC 2 cut(s) 1003, 1013
AfiI CCNNNNNNNGG 1 cut(s) 1427
AflII CTTAAG 1 cut(s) 638
AgsI TTSAA 8 cut(s) 176, 212, 401, 797, 1139, 1196, 1247, 1376
AjnI CCWGG 1 cut(s) 977
AleI CACNNNNGTG 1 cut(s) 756
Alw21I GWGCWC 1 cut(s) 637
Alw26I GTCTC 3 cut(s) 425, 977, 1164
AlwI GGATC 2 cut(s) 944, 946
AlwNI CAGNNNCTG 1 cut(s) 654
AoxI GGCC 1 cut(s) 838
ApeKI GCWGC 3 cut(s) 1040, 1169, 1238
ApoI RAATTY 3 cut(s) 170, 990, 1508
Asp718I GGTACC 1 cut(s) 1011
AspLEI GCGC 4 cut(s) 56, 73, 408, 1174
AspS9I GGNCC 3 cut(s) 626, 839, 1418
AsuHPI GGTGA 1 cut(s) 1244
AsuII TTCGAA 1 cut(s) 943
AvaII GGWCC 2 cut(s) 626, 1418
AxyI CCTNAGG 1 cut(s) 609
BanI GGYRCC 1 cut(s) 1011
BarI GAAGNNNNNNTAC 2 cut(s) 588, 620
Bbv12I GWGCWC 1 cut(s) 637
BbvI GCAGC 3 cut(s) 1027, 1156, 1225
BccI CCATC 1 cut(s) 922
BceAI ACGGC 2 cut(s) 41, 1406
BcgI CGANNNNNNTGC 1 cut(s) 1508
BciT130I CCWGG 1 cut(s) 979
BclI TGATCA 1 cut(s) 502
BcoDI GTCTC 3 cut(s) 425, 977, 1164
BfaI CTAG 5 cut(s) 74, 528, 575, 734, 1487
BfmI CTRYAG 2 cut(s) 459, 1239
BfrI CTTAAG 1 cut(s) 638
BisI GCNGC 4 cut(s) 1041, 1170, 1236, 1239
BlsI GCNGC 4 cut(s) 1042, 1171, 1237, 1240
BmcAI AGTACT 1 cut(s) 1003
Bme1390I CCNGG 1 cut(s) 979
Bme18I GGWCC 2 cut(s) 626, 1418
BmgT120I GGNCC 3 cut(s) 626, 839, 1418
BmiI GGNNCC 5 cut(s) 628, 841, 1013, 1399, 1420
BmrFI CCNGG 1 cut(s) 979
BmrI ACTGGG 1 cut(s) 1508
BmsI GCATC 6 cut(s) 189, 213, 420, 694, 884, 1130
BmuI ACTGGG 1 cut(s) 1508
BpmI CTGGAG 1 cut(s) 37
Bpu14I TTCGAA 1 cut(s) 943
BpuEI CTTGAG 1 cut(s) 1361
BsaAI YACGTR 1 cut(s) 135
BsaBI GATNNNNATC 1 cut(s) 555
BsaI GGTCTC 1 cut(s) 425
BsaJI CCNNGG 5 cut(s) 801, 977, 978, 1222, 1403
BsaXI ACNNNNNCTCC 2 cut(s) 1270, 1300
Bsc4I CCNNNNNNNGG 1 cut(s) 1427
Bse1I ACTGG 3 cut(s) 288, 991, 1514
Bse21I CCTNAGG 1 cut(s) 609
Bse3DI GCAATG 2 cut(s) 350, 1035
Bse8I GATNNNNATC 1 cut(s) 555
BseBI CCWGG 1 cut(s) 979
BseDI CCNNGG 5 cut(s) 801, 977, 978, 1222, 1403
BseGI GGATG 4 cut(s) 286, 307, 831, 899
BseJI GATNNNNATC 1 cut(s) 555
BseLI CCNNNNNNNGG 1 cut(s) 1427
BseMI GCAATG 2 cut(s) 350, 1035
BseMII CTCAG 1 cut(s) 1012
BseNI ACTGG 3 cut(s) 288, 991, 1514
BseRI GAGGAG 3 cut(s) 22, 25, 1145
BseXI GCAGC 3 cut(s) 1027, 1156, 1225
BseYI CCCAGC 1 cut(s) 654
BsgI GTGCAG 1 cut(s) 456
BshFI GGCC 1 cut(s) 840
BshNI GGYRCC 1 cut(s) 1011
BsiHKAI GWGCWC 1 cut(s) 637
BslFI GGGAC 5 cut(s) 86, 315, 345, 612, 1404
BslI CCNNNNNNNGG 1 cut(s) 1427
BsmAI GTCTC 3 cut(s) 425, 977, 1164
BsmBI CGTCTC 1 cut(s) 1164
BsmFI GGGAC 5 cut(s) 86, 315, 345, 612, 1404
BsmI GAATGC 1 cut(s) 182
BsnI GGCC 1 cut(s) 840
Bso31I GGTCTC 1 cut(s) 425
Bsp119I TTCGAA 1 cut(s) 943
Bsp1286I GDGCHC 1 cut(s) 637
Bsp143I GATC 6 cut(s) 502, 556, 820, 936, 951, 1075
Bsp19I CCATGG 1 cut(s) 801
BspACI CCGC 4 cut(s) 380, 660, 776, 1235
BspANI GGCC 1 cut(s) 840
BspCNI CTCAG 1 cut(s) 1011
BspLI GGNNCC 5 cut(s) 628, 841, 1013, 1399, 1420
BspMAI CTGCAG 1 cut(s) 1243
BspPI GGATC 2 cut(s) 944, 946
BspQI GCTCTTC 1 cut(s) 1097
BspT104I TTCGAA 1 cut(s) 943
BspT107I GGYRCC 1 cut(s) 1011
BspTI CTTAAG 1 cut(s) 638
BspTNI GGTCTC 1 cut(s) 425
BsrDI GCAATG 2 cut(s) 350, 1035
BsrI ACTGG 3 cut(s) 288, 991, 1514
BssECI CCNNGG 5 cut(s) 801, 977, 978, 1222, 1403
BssMI GATC 6 cut(s) 502, 556, 820, 936, 951, 1075
BssT1I CCWWGG 1 cut(s) 801
Bst2UI CCWGG 1 cut(s) 979
Bst4CI ACNGT 5 cut(s) 34, 148, 510, 755, 1256
Bst6I CTCTTC 4 cut(s) 89, 1097, 1403, 1408
BstAFI CTTAAG 1 cut(s) 638
BstBAI YACGTR 1 cut(s) 135
BstBI TTCGAA 1 cut(s) 943
BstC8I GCNNGC 4 cut(s) 435, 709, 882, 899
BstDEI CTNAG 5 cut(s) 609, 712, 932, 998, 1433
BstDSI CCRYGG 2 cut(s) 801, 1403
BstEII GGTNACC 1 cut(s) 1250
BstF5I GGATG 4 cut(s) 286, 307, 831, 899
BstHHI GCGC 4 cut(s) 56, 73, 408, 1174
BstKTI GATC 6 cut(s) 505, 559, 823, 939, 954, 1078
BstMAI GTCTC 3 cut(s) 425, 977, 1164
BstMBI GATC 6 cut(s) 502, 556, 820, 936, 951, 1075
BstMWI GCNNNNNNNGC 2 cut(s) 60, 77
BstNI CCWGG 1 cut(s) 979
BstNSI RCATGY 1 cut(s) 880
BstPI GGTNACC 1 cut(s) 1250
BstSCI CCNGG 1 cut(s) 977
BstSFI CTRYAG 2 cut(s) 459, 1239
BstV1I GCAGC 3 cut(s) 1027, 1156, 1225
BstX2I RGATCY 1 cut(s) 951
BstYI RGATCY 1 cut(s) 951
Bsu36I CCTNAGG 1 cut(s) 609
BsuRI GGCC 1 cut(s) 840
BtgI CCRYGG 2 cut(s) 801, 1403
BtsCI GGATG 4 cut(s) 286, 307, 831, 899
BtsIMutI CAGTG 3 cut(s) 39, 751, 1521
Cac8I GCNNGC 4 cut(s) 435, 709, 882, 899
CaiI CAGNNNCTG 1 cut(s) 654
CfoI GCGC 4 cut(s) 56, 73, 408, 1174
Cfr13I GGNCC 3 cut(s) 626, 839, 1418
CseI GACGC 1 cut(s) 1146
Csp6I GTAC 2 cut(s) 1002, 1012
CviAII CATG 6 cut(s) 190, 451, 802, 877, 1154, 1231
CviQI GTAC 2 cut(s) 1002, 1012
DdeI CTNAG 5 cut(s) 609, 712, 932, 998, 1433
DpnI GATC 6 cut(s) 504, 558, 822, 938, 953, 1077
DpnII GATC 6 cut(s) 502, 556, 820, 936, 951, 1075
Eam1104I CTCTTC 4 cut(s) 89, 1097, 1403, 1408
EarI CTCTTC 4 cut(s) 89, 1097, 1403, 1408
Eco130I CCWWGG 1 cut(s) 801
Eco31I GGTCTC 1 cut(s) 425
Eco47I GGWCC 2 cut(s) 626, 1418
Eco57I CTGAAG 2 cut(s) 939, 1121
Eco81I CCTNAGG 1 cut(s) 609
Eco91I GGTNACC 1 cut(s) 1250
EcoO109I RGGNCCY 1 cut(s) 1418
EcoO65I GGTNACC 1 cut(s) 1250
EcoRI GAATTC 1 cut(s) 1508
EcoRII CCWGG 1 cut(s) 977
EcoT14I CCWWGG 1 cut(s) 801
EcoT22I ATGCAT 1 cut(s) 182
ErhI CCWWGG 1 cut(s) 801
Esp3I CGTCTC 1 cut(s) 1164
FaeI CATG 6 cut(s) 193, 454, 805, 880, 1157, 1234
FaqI GGGAC 5 cut(s) 86, 315, 345, 612, 1404
FatI CATG 6 cut(s) 189, 450, 801, 876, 1153, 1230
FauNDI CATATG 1 cut(s) 1068
FbaI TGATCA 1 cut(s) 502
Fnu4HI GCNGC 4 cut(s) 1041, 1170, 1236, 1239
FokI GGATG 4 cut(s) 293, 314, 838, 906
Fsp4HI GCNGC 4 cut(s) 1041, 1170, 1236, 1239
FspAI RTGCGCAY 1 cut(s) 407
FspBI CTAG 5 cut(s) 74, 528, 575, 734, 1487
FspI TGCGCA 1 cut(s) 407
GlaI GCGC 4 cut(s) 55, 72, 407, 1173
GluI GCNGC 4 cut(s) 1041, 1170, 1236, 1239
GsaI CCCAGC 1 cut(s) 658
GsuI CTGGAG 1 cut(s) 37
HaeIII GGCC 1 cut(s) 840
HgaI GACGC 1 cut(s) 1146
HhaI GCGC 4 cut(s) 56, 73, 408, 1174
Hin1II CATG 6 cut(s) 193, 454, 805, 880, 1157, 1234
Hin6I GCGC 4 cut(s) 54, 71, 406, 1172
HinP1I GCGC 4 cut(s) 54, 71, 406, 1172
HindIII AAGCTT 2 cut(s) 602, 641
HinfI GANTC 6 cut(s) 208, 319, 328, 383, 524, 1061
HphI GGTGA 1 cut(s) 1244
Hpy166II GTNNAC 1 cut(s) 718
Hpy188I TCNGA 7 cut(s) 115, 429, 650, 825, 919, 1226, 1459
Hpy188III TCNNGA 6 cut(s) 332, 494, 734, 955, 1289, 1340
Hpy8I GTNNAC 1 cut(s) 718
Hpy99I CGWCG 1 cut(s) 1260
HpyAV CCTTC 6 cut(s) 34, 863, 865, 1241, 1340, 1435
HpyCH4III ACNGT 5 cut(s) 34, 148, 510, 755, 1256
HpyCH4IV ACGT 3 cut(s) 50, 134, 585
HpyCH4V TGCA 9 cut(s) 4, 180, 343, 437, 897, 1241, 1381, 1427, 1519
HpyF10VI GCNNNNNNNGC 2 cut(s) 60, 77
HpyF3I CTNAG 5 cut(s) 609, 712, 932, 998, 1433
HpySE526I ACGT 3 cut(s) 50, 134, 585
Hsp92II CATG 6 cut(s) 193, 454, 805, 880, 1157, 1234
HspAI GCGC 4 cut(s) 54, 71, 406, 1172
KpnI GGTACC 1 cut(s) 1015
Ksp22I TGATCA 1 cut(s) 502
Kzo9I GATC 6 cut(s) 502, 556, 820, 936, 951, 1075
LguI GCTCTTC 1 cut(s) 1097
LmnI GCTCC 5 cut(s) 12, 132, 778, 1132, 1403
Lsp1109I GCAGC 3 cut(s) 1027, 1156, 1225
LweI GCATC 6 cut(s) 189, 213, 420, 694, 884, 1130
MaeI CTAG 5 cut(s) 74, 528, 575, 734, 1487
MaeII ACGT 3 cut(s) 50, 134, 585
MaeIII GTNAC 3 cut(s) 384, 446, 1250
MalI GATC 6 cut(s) 504, 558, 822, 938, 953, 1077
MboI GATC 6 cut(s) 502, 556, 820, 936, 951, 1075
MboII GAAGA 9 cut(s) 76, 176, 179, 328, 566, 1114, 1420, 1425, 1472
MfeI CAATTG 2 cut(s) 66, 1125
MflI RGATCY 1 cut(s) 951
MhlI GDGCHC 1 cut(s) 637
MlyI GAGTC 3 cut(s) 337, 392, 518
MmeI TCCRAC 3 cut(s) 138, 258, 272
Mph1103I ATGCAT 1 cut(s) 182
MseI TTAA 5 cut(s) 488, 588, 639, 908, 971
MslI CAYNNNNRTG 1 cut(s) 756
MspCI CTTAAG 1 cut(s) 638
MspR9I CCNGG 1 cut(s) 979
MunI CAATTG 2 cut(s) 66, 1125
Mva1269I GAATGC 1 cut(s) 182
MvaI CCWGG 1 cut(s) 979
MwoI GCNNNNNNNGC 2 cut(s) 60, 77
NcoI CCATGG 1 cut(s) 801
NdeI CATATG 1 cut(s) 1068
NdeII GATC 6 cut(s) 502, 556, 820, 936, 951, 1075
NlaIII CATG 6 cut(s) 193, 454, 805, 880, 1157, 1234
NlaIV GGNNCC 5 cut(s) 628, 841, 1013, 1399, 1420
NmuCI GTSAC 3 cut(s) 384, 446, 1250
NsbI TGCGCA 1 cut(s) 407
NsiI ATGCAT 1 cut(s) 182
NspI RCATGY 1 cut(s) 880
NspV TTCGAA 1 cut(s) 943
OliI CACNNNNGTG 1 cut(s) 756
PasI CCCWGGG 1 cut(s) 978
PciSI GCTCTTC 1 cut(s) 1097
PctI GAATGC 1 cut(s) 182
PfeI GAWTC 3 cut(s) 208, 319, 1061
PflFI GACNNNGTC 1 cut(s) 119
PkrI GCNGC 4 cut(s) 1042, 1171, 1237, 1240
PleI GAGTC 3 cut(s) 336, 391, 518
PpsI GAGTC 3 cut(s) 336, 391, 518
Ppu21I YACGTR 1 cut(s) 135
PpuMI RGGWCCY 1 cut(s) 1418
Psp5II RGGWCCY 1 cut(s) 1418
Psp6I CCWGG 1 cut(s) 977
PspEI GGTNACC 1 cut(s) 1250
PspFI CCCAGC 1 cut(s) 654
PspGI CCWGG 1 cut(s) 977
PspN4I GGNNCC 5 cut(s) 628, 841, 1013, 1399, 1420
PspPI GGNCC 3 cut(s) 626, 839, 1418
PspPPI RGGWCCY 1 cut(s) 1418
PstI CTGCAG 1 cut(s) 1243
PstNI CAGNNNCTG 1 cut(s) 654
PsuI RGATCY 1 cut(s) 951
PsyI GACNNNGTC 1 cut(s) 119
RsaI GTAC 2 cut(s) 1003, 1013
RsaNI GTAC 2 cut(s) 1002, 1012
RseI CAYNNNNRTG 1 cut(s) 756
SapI GCTCTTC 1 cut(s) 1097
SaqAI TTAA 5 cut(s) 488, 588, 639, 908, 971
SatI GCNGC 4 cut(s) 1041, 1170, 1236, 1239
Sau3AI GATC 6 cut(s) 502, 556, 820, 936, 951, 1075
Sau96I GGNCC 3 cut(s) 626, 839, 1418
ScaI AGTACT 1 cut(s) 1003
SchI GAGTC 3 cut(s) 337, 392, 518
ScrFI CCNGG 1 cut(s) 979
SduI GDGCHC 1 cut(s) 637
SfaNI GCATC 6 cut(s) 189, 213, 420, 694, 884, 1130
SfcI CTRYAG 2 cut(s) 459, 1239
SfuI TTCGAA 1 cut(s) 943
SinI GGWCC 2 cut(s) 626, 1418
SmiMI CAYNNNNRTG 1 cut(s) 756
SmlI CTYRAG 2 cut(s) 638, 1340
SmoI CTYRAG 2 cut(s) 638, 1340
SsiI CCGC 4 cut(s) 380, 660, 776, 1235
SspMI CTAG 5 cut(s) 74, 528, 575, 734, 1487
StyD4I CCNGG 1 cut(s) 977
StyI CCWWGG 1 cut(s) 801
TaaI ACNGT 5 cut(s) 34, 148, 510, 755, 1256
TaiI ACGT 3 cut(s) 53, 137, 588
TaqI TCGA 1 cut(s) 943
TatI WGTACW 1 cut(s) 1001
TauI GCSGC 1 cut(s) 1238
TfiI GAWTC 3 cut(s) 208, 319, 1061
Tru1I TTAA 5 cut(s) 488, 588, 639, 908, 971
Tru9I TTAA 5 cut(s) 488, 588, 639, 908, 971
TscAI CASTG 3 cut(s) 39, 758, 1521
TseFI GTSAC 3 cut(s) 384, 446, 1250
TseI GCWGC 3 cut(s) 1040, 1169, 1238
Tsp45I GTSAC 3 cut(s) 384, 446, 1250
TspDTI ATGAA 5 cut(s) 178, 567, 783, 845, 942
TspGWI ACGGA 2 cut(s) 229, 1420
TspRI CASTG 3 cut(s) 39, 758, 1521
Tth111I GACNNNGTC 1 cut(s) 119
Vha464I CTTAAG 1 cut(s) 638
VpaK11BI GGWCC 2 cut(s) 626, 1418
XapI RAATTY 3 cut(s) 170, 990, 1508
XbaI TCTAGA 1 cut(s) 733
XceI RCATGY 1 cut(s) 880
XcmI CCANNNNNNNNNTGG 1 cut(s) 295
XspI CTAG 5 cut(s) 74, 528, 575, 734, 1487
ZrmI AGTACT 1 cut(s) 1003
Zsp2I ATGCAT 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.