MD15G1423500.v1.1
ERF Family

2OG-Fe(II) oxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0022335
Physical Location & Seq
Reverse (-)
52474152 .. 52478029
3878 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1423500.v1.1.491

Sequence Viewer

Length: 1398 bp
ATGACGATGATGATGGAGGATGGCCAGGGCCAAGATTGGAGGAGCTTGGCGAGGACTTTAGAACCCGACCAAATTCTGGAAGTTTTGTCAGGCGGGTTTTGCAGCAACTGCCAGCTCCTTTTGGAGAGTCGAATTCACACCCTCCTCAACAAAAAAGTAGGTGAGAAGGTATCTTTTTCCAGTGATGATGATAATAATAGTTCTGCACAATTGAAGTCCATGGATGCACAAACCCCTTCACCATGCCAGTGTGTGGACGAACCAATTGTGCCGTCCTCAGAGAATACTACGGAGTCCTTCGATAAAATGCTTGACCAGCAAGAGTGCACTCCAATCCAAACACCTTATCATACAACTCCTGTCCACTCATCAATCCAAATAGAGTGCACGTCTAATTCATCTCCTCCGATGTTATTCGCTGGTGCAAAAAGTGGAGATTCCTCGGAGGATCCAAACGAGCATAACCGAGTATTACAAGTTCGGAGACAGAACTTCGTCCACATTGAGTACAGACATGGCAGACGAATAAATGTCTTACAGGGCCTAGAGCTACATACTCGGGTTTTTGATGCCGAGGAGCAGAAGCAAATTATCGAGTGTGTCTACAATTTACAACGTATGGGGAAAAGTGGACAACTTAGAGCGCGTACGTATAGCGAACCTAGGAAGTGGATGCGTGGTAAAGGACGTGTGACCATACAATTTGGCTGTTGTTACAATTATGCAGTGGATAAGAATGGGAACCGGCCAGGTATCGTTAGAGATGAAGATGTTGATCCTCTACCTCCTTTGTTCAAGCAAATGATAAAGAGGATGGTTGGTTGGCATATATTACCTTCATCATGCATCCCGGACAGCTGCATTGTGAATATTTATGACGAAGGGGATTGCATTCCTCCTCATATTGACCACGATGATTTTGTTAGACCGTTTTGCACGGTTTCACTTTTGGCCGAATGCAAGATACTCTTCGGTTCAAATCTAAAGGTTGTAGGACCTGGAGACTTTTCAGGACCTTATTCTATACGTTTGCCCGTCGGATCGGTGCTTCTTCTAGATGGTAATGCAGCTAACGTTGCTAAGCATTGCATACCTGGTGTTCCGGCAAGAAGGATTTCGATTACATTCAGAAAAATGGATAGTAGCAAGCTACCGCACAATTTTTTCCATGATCCCGAGCTTCAGGGAATTAAACCTTATGCTGTTTCTCCATTTGCCAAATCAAAAACCCAGCAAGATCAACGAGAGAAGCATTATTCCTATCTTCCTAGACGCAACCGTGGTGAATTCAAGAACGCTAAGAAGCGGAACCGCAACTCTTTTCTTCTTGAGAAATATGAGTTCTCTCCACCTGGGAGATTCAGTTCTGGGAACAGACGGATGTTCAACAGACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy_2

Protein Analysis

466

Amino Acids

52.83

Weight (kDa)

8.63

Isoelectric Point (pI)

61.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_2 PF13532 184 - 377 3e-12 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022335)

Species Orthologous Gene IDs
malus_domestica MD15G1423500.v1.1
prunus_persica Prupe.1G569500_v2.0.a1 Prupe.1G569500_v2.0.a1
pyrus_communis pycom15g37550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 76, 253
AccI GTMKAC 1 cut(s) 603
AccII CGCG 1 cut(s) 646
AciI CCGC 4 cut(s) 93, 1154, 1306, 1312
AclI AACGTT 1 cut(s) 1074
AclWI GGATC 5 cut(s) 443, 456, 770, 1048, 1166
AcoI YGGCCR 3 cut(s) 22, 746, 951
AcsI RAATTY 3 cut(s) 72, 132, 1286
AcuI CTGAAG 1 cut(s) 1166
AfaI GTAC 2 cut(s) 509, 649
AfiI CCNNNNNNNGG 2 cut(s) 76, 253
AflIII ACRYGT 1 cut(s) 688
AgsI TTSAA 5 cut(s) 214, 796, 978, 1291, 1387
AjiI CACGTC 2 cut(s) 390, 689
AjnI CCWGG 5 cut(s) 24, 748, 997, 1093, 1351
AloI GAACNNNNNNTCC 2 cut(s) 1348, 1380
AluBI AGCT 7 cut(s) 45, 115, 550, 858, 1070, 1150, 1180
AluI AGCT 7 cut(s) 45, 115, 550, 858, 1070, 1150, 1180
Alw21I GWGCWC 2 cut(s) 329, 389
Alw26I GTCTC 2 cut(s) 478, 996
Alw44I GTGCAC 2 cut(s) 325, 385
AlwI GGATC 5 cut(s) 443, 456, 770, 1048, 1166
AlwNI CAGNNNCTG 1 cut(s) 108
Ama87I CYCGRG 2 cut(s) 558, 1175
AoxI GGCC 5 cut(s) 22, 28, 541, 746, 951
ApaLI GTGCAC 2 cut(s) 325, 385
ApeKI GCWGC 3 cut(s) 102, 858, 1067
ApoI RAATTY 3 cut(s) 72, 132, 1286
Asp700I GAANNNNTTC 1 cut(s) 1114
AspA2I CCTAGG 1 cut(s) 662
AspLEI GCGC 1 cut(s) 646
AspS9I GGNCC 4 cut(s) 28, 541, 995, 1013
AsuC2I CCSGG 1 cut(s) 851
AsuHPI GGTGA 3 cut(s) 173, 231, 1295
AvaI CYCGRG 2 cut(s) 558, 1175
AvaII GGWCC 2 cut(s) 995, 1013
AvrII CCTAGG 1 cut(s) 662
BaeGI GKGCMC 2 cut(s) 329, 389
BalI TGGCCA 1 cut(s) 24
BamHI GGATCC 1 cut(s) 448
Bbv12I GWGCWC 2 cut(s) 329, 389
BbvI GCAGC 3 cut(s) 114, 845, 1079
BccI CCATC 4 cut(s) 7, 14, 808, 1052
BceAI ACGGC 1 cut(s) 256
BciT130I CCWGG 5 cut(s) 26, 750, 999, 1095, 1353
BcnI CCSGG 1 cut(s) 851
BcoDI GTCTC 2 cut(s) 478, 996
BfaI CTAG 4 cut(s) 545, 663, 1055, 1269
BisI GCNGC 3 cut(s) 103, 859, 1068
BlnI CCTAGG 1 cut(s) 662
BlpI GCTNAGC 1 cut(s) 1080
BlsI GCNGC 3 cut(s) 104, 860, 1069
Bme1390I CCNGG 6 cut(s) 26, 750, 851, 999, 1095, 1353
Bme18I GGWCC 2 cut(s) 995, 1013
BmeT110I CYCGRG 2 cut(s) 558, 1175
BmgBI CACGTC 2 cut(s) 390, 689
BmgT120I GGNCC 4 cut(s) 28, 541, 995, 1013
BmiI GGNNCC 3 cut(s) 450, 743, 1310
BmrFI CCNGG 6 cut(s) 26, 750, 851, 999, 1095, 1353
BmsI GCATC 4 cut(s) 214, 559, 663, 855
BpmI CTGGAG 1 cut(s) 1020
Bpu1102I GCTNAGC 1 cut(s) 1080
BpuEI CTTGAG 1 cut(s) 1349
BpuMI CCSGG 1 cut(s) 851
BsaAI YACGTR 1 cut(s) 651
BsaBI GATNNNNATC 1 cut(s) 774
BsaJI CCNNGG 7 cut(s) 25, 219, 441, 573, 662, 1279, 1352
BsaXI ACNNNNNCTCC 2 cut(s) 1348, 1378
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 253
Bse118I RCCGGY 1 cut(s) 744
Bse1I ACTGG 2 cut(s) 180, 247
Bse3DI GCAATG 1 cut(s) 1084
Bse8I GATNNNNATC 1 cut(s) 774
BseBI CCWGG 5 cut(s) 26, 750, 999, 1095, 1353
BseDI CCNNGG 7 cut(s) 25, 219, 441, 573, 662, 1279, 1352
BseGI GGATG 6 cut(s) 25, 229, 678, 819, 846, 1386
BseJI GATNNNNATC 1 cut(s) 774
BseLI CCNNNNNNNGG 2 cut(s) 76, 253
BseMI GCAATG 1 cut(s) 1084
BseMII CTCAG 1 cut(s) 291
BseNI ACTGG 2 cut(s) 180, 247
BseRI GAGGAG 5 cut(s) 55, 134, 393, 590, 888
BseSI GKGCMC 2 cut(s) 329, 389
BseXI GCAGC 3 cut(s) 114, 845, 1079
BseYI CCCAGC 1 cut(s) 1230
BsgI GTGCAG 1 cut(s) 189
Bsh1236I CGCG 1 cut(s) 646
BshFI GGCC 5 cut(s) 24, 30, 543, 748, 953
BsiHKAI GWGCWC 2 cut(s) 329, 389
BsiHKCI CYCGRG 2 cut(s) 558, 1175
BsiSI CCGG 3 cut(s) 745, 851, 1103
BsiWI CGTACG 1 cut(s) 647
BslI CCNNNNNNNGG 2 cut(s) 76, 253
BsmAI GTCTC 2 cut(s) 478, 996
BsmI GAATGC 2 cut(s) 891, 962
BsnI GGCC 5 cut(s) 24, 30, 543, 748, 953
BsoBI CYCGRG 2 cut(s) 558, 1175
Bsp1286I GDGCHC 2 cut(s) 329, 389
Bsp143I GATC 5 cut(s) 448, 775, 1040, 1171, 1237
Bsp1720I GCTNAGC 1 cut(s) 1080
Bsp19I CCATGG 1 cut(s) 219
BspACI CCGC 4 cut(s) 93, 1154, 1306, 1312
BspANI GGCC 5 cut(s) 24, 30, 543, 748, 953
BspCNI CTCAG 1 cut(s) 290
BspFNI CGCG 1 cut(s) 646
BspLI GGNNCC 3 cut(s) 450, 743, 1310
BspPI GGATC 5 cut(s) 443, 456, 770, 1048, 1166
BsrDI GCAATG 1 cut(s) 1084
BsrFI RCCGGY 1 cut(s) 744
BsrI ACTGG 2 cut(s) 180, 247
BssAI RCCGGY 1 cut(s) 744
BssECI CCNNGG 7 cut(s) 25, 219, 441, 573, 662, 1279, 1352
BssMI GATC 5 cut(s) 448, 775, 1040, 1171, 1237
BssT1I CCWWGG 2 cut(s) 219, 662
Bst2UI CCWGG 5 cut(s) 26, 750, 999, 1095, 1353
Bst4CI ACNGT 4 cut(s) 930, 940, 1280, 1395
Bst6I CTCTTC 1 cut(s) 974
BstAPI GCANNNNNTGC 1 cut(s) 108
BstBAI YACGTR 1 cut(s) 651
BstC8I GCNNGC 2 cut(s) 113, 1148
BstDEI CTNAG 4 cut(s) 277, 638, 1080, 1299
BstDSI CCRYGG 2 cut(s) 219, 1279
BstF5I GGATG 6 cut(s) 25, 229, 678, 819, 846, 1386
BstFNI CGCG 1 cut(s) 646
BstHHI GCGC 1 cut(s) 646
BstKTI GATC 5 cut(s) 451, 778, 1043, 1174, 1240
BstMAI GTCTC 2 cut(s) 478, 996
BstMBI GATC 5 cut(s) 448, 775, 1040, 1171, 1237
BstMWI GCNNNNNNNGC 4 cut(s) 99, 108, 316, 1076
BstNI CCWGG 5 cut(s) 26, 750, 999, 1095, 1353
BstSCI CCNGG 6 cut(s) 24, 748, 849, 997, 1093, 1351
BstSLI GKGCMC 2 cut(s) 329, 389
BstSNI TACGTA 1 cut(s) 651
BstUI CGCG 1 cut(s) 646
BstV1I GCAGC 3 cut(s) 114, 845, 1079
BstX2I RGATCY 1 cut(s) 448
BstYI RGATCY 1 cut(s) 448
BsuRI GGCC 5 cut(s) 24, 30, 543, 748, 953
BtgI CCRYGG 2 cut(s) 219, 1279
BtrI CACGTC 2 cut(s) 390, 689
BtsCI GGATG 6 cut(s) 25, 229, 678, 819, 846, 1386
BtsI GCAGTG 1 cut(s) 732
BtsIMutI CAGTG 3 cut(s) 187, 254, 732
Cac8I GCNNGC 2 cut(s) 113, 1148
CaiI CAGNNNCTG 1 cut(s) 108
CfoI GCGC 1 cut(s) 646
Cfr10I RCCGGY 1 cut(s) 744
Cfr13I GGNCC 4 cut(s) 28, 541, 995, 1013
CseI GACGC 1 cut(s) 1281
CsiI ACCWGGT 1 cut(s) 1093
Csp6I GTAC 2 cut(s) 508, 648
CviAII CATG 5 cut(s) 220, 243, 515, 843, 1169
CviQI GTAC 2 cut(s) 508, 648
DdeI CTNAG 4 cut(s) 277, 638, 1080, 1299
DpnI GATC 5 cut(s) 450, 777, 1042, 1173, 1239
DpnII GATC 5 cut(s) 448, 775, 1040, 1171, 1237
EaeI YGGCCR 3 cut(s) 22, 746, 951
Eam1104I CTCTTC 1 cut(s) 974
EarI CTCTTC 1 cut(s) 974
Eco105I TACGTA 1 cut(s) 651
Eco130I CCWWGG 2 cut(s) 219, 662
Eco47I GGWCC 2 cut(s) 995, 1013
Eco57I CTGAAG 1 cut(s) 1166
Eco88I CYCGRG 2 cut(s) 558, 1175
EcoO109I RGGNCCY 3 cut(s) 541, 995, 1013
EcoRI GAATTC 2 cut(s) 132, 1286
EcoRII CCWGG 5 cut(s) 24, 748, 997, 1093, 1351
EcoT14I CCWWGG 2 cut(s) 219, 662
EcoT22I ATGCAT 1 cut(s) 848
ErhI CCWWGG 2 cut(s) 219, 662
FaeI CATG 5 cut(s) 223, 246, 518, 846, 1172
FalI AAGNNNNNCTT 2 cut(s) 953, 985
FatI CATG 5 cut(s) 219, 242, 514, 842, 1168
FauI CCCGC 1 cut(s) 86
FblI GTMKAC 1 cut(s) 603
Fnu4HI GCNGC 3 cut(s) 103, 859, 1068
FokI GGATG 6 cut(s) 32, 236, 685, 826, 833, 1393
Fsp4HI GCNGC 3 cut(s) 103, 859, 1068
FspBI CTAG 4 cut(s) 545, 663, 1055, 1269
GlaI GCGC 1 cut(s) 645
GluI GCNGC 3 cut(s) 103, 859, 1068
GsaI CCCAGC 1 cut(s) 1234
GsuI CTGGAG 1 cut(s) 1020
HaeIII GGCC 5 cut(s) 24, 30, 543, 748, 953
HapII CCGG 3 cut(s) 745, 851, 1103
HgaI GACGC 1 cut(s) 1281
HhaI GCGC 1 cut(s) 646
Hin1II CATG 5 cut(s) 223, 246, 518, 846, 1172
Hin6I GCGC 1 cut(s) 644
HinP1I GCGC 1 cut(s) 644
HinfI GANTC 4 cut(s) 127, 293, 437, 1359
HpaII CCGG 3 cut(s) 745, 851, 1103
HphI GGTGA 3 cut(s) 173, 231, 1295
Hpy166II GTNNAC 7 cut(s) 256, 327, 364, 387, 499, 604, 632
Hpy188I TCNGA 6 cut(s) 280, 408, 445, 483, 1040, 1130
Hpy188III TCNNGA 6 cut(s) 77, 1011, 1055, 1175, 1291, 1328
Hpy8I GTNNAC 7 cut(s) 256, 327, 364, 387, 499, 604, 632
Hpy99I CGWCG 1 cut(s) 1040
HpyAV CCTTC 6 cut(s) 160, 246, 307, 846, 875, 1104
HpyCH4III ACNGT 4 cut(s) 930, 940, 1280, 1395
HpyCH4IV ACGT 6 cut(s) 389, 616, 650, 688, 1027, 1074
HpyF10VI GCNNNNNNNGC 4 cut(s) 99, 108, 316, 1076
HpyF3I CTNAG 4 cut(s) 277, 638, 1080, 1299
HpySE526I ACGT 6 cut(s) 389, 616, 650, 688, 1027, 1074
Hsp92II CATG 5 cut(s) 223, 246, 518, 846, 1172
HspAI GCGC 1 cut(s) 644
Kzo9I GATC 5 cut(s) 448, 775, 1040, 1171, 1237
LmnI GCTCC 3 cut(s) 42, 120, 577
Lsp1109I GCAGC 3 cut(s) 114, 845, 1079
LweI GCATC 4 cut(s) 214, 559, 663, 855
MabI ACCWGGT 1 cut(s) 1093
MaeI CTAG 4 cut(s) 545, 663, 1055, 1269
MaeII ACGT 6 cut(s) 389, 616, 650, 688, 1027, 1074
MaeIII GTNAC 2 cut(s) 691, 713
MalI GATC 5 cut(s) 450, 777, 1042, 1173, 1239
MboI GATC 5 cut(s) 448, 775, 1040, 1171, 1237
MboII GAAGA 5 cut(s) 779, 961, 1043, 1256, 1316
MfeI CAATTG 2 cut(s) 209, 264
MflI RGATCY 1 cut(s) 448
MhlI GDGCHC 2 cut(s) 329, 389
MlsI TGGCCA 1 cut(s) 24
MluNI TGGCCA 1 cut(s) 24
MlyI GAGTC 2 cut(s) 136, 302
MmeI TCCRAC 1 cut(s) 1018
Mox20I TGGCCA 1 cut(s) 24
Mph1103I ATGCAT 1 cut(s) 848
MroXI GAANNNNTTC 1 cut(s) 1114
MscI TGGCCA 1 cut(s) 24
MseI TTAA 1 cut(s) 1191
MslI CAYNNNNRTG 1 cut(s) 247
Msp20I TGGCCA 1 cut(s) 24
MspA1I CMGCKG 1 cut(s) 858
MspI CCGG 3 cut(s) 745, 851, 1103
MspR9I CCNGG 6 cut(s) 26, 750, 851, 999, 1095, 1353
MunI CAATTG 2 cut(s) 209, 264
Mva1269I GAATGC 2 cut(s) 891, 962
MvaI CCWGG 5 cut(s) 26, 750, 999, 1095, 1353
MvnI CGCG 1 cut(s) 646
MwoI GCNNNNNNNGC 4 cut(s) 99, 108, 316, 1076
NciI CCSGG 1 cut(s) 851
NcoI CCATGG 1 cut(s) 219
NdeII GATC 5 cut(s) 448, 775, 1040, 1171, 1237
NlaIII CATG 5 cut(s) 223, 246, 518, 846, 1172
NlaIV GGNNCC 3 cut(s) 450, 743, 1310
NmeAIII GCCGAG 1 cut(s) 598
NmuCI GTSAC 1 cut(s) 691
NsiI ATGCAT 1 cut(s) 848
PctI GAATGC 2 cut(s) 891, 962
PdmI GAANNNNTTC 1 cut(s) 1114
PfeI GAWTC 2 cut(s) 437, 1359
Pfl23II CGTACG 1 cut(s) 647
PflMI CCANNNNNTGG 2 cut(s) 76, 253
PfoI TCCNGGA 1 cut(s) 849
PkrI GCNGC 3 cut(s) 104, 860, 1069
PleI GAGTC 2 cut(s) 135, 301
PpsI GAGTC 2 cut(s) 135, 301
Ppu21I YACGTR 1 cut(s) 651
PpuMI RGGWCCY 2 cut(s) 995, 1013
Psp1406I AACGTT 1 cut(s) 1074
Psp5II RGGWCCY 2 cut(s) 995, 1013
Psp6I CCWGG 5 cut(s) 24, 748, 997, 1093, 1351
PspFI CCCAGC 1 cut(s) 1230
PspGI CCWGG 5 cut(s) 24, 748, 997, 1093, 1351
PspLI CGTACG 1 cut(s) 647
PspN4I GGNNCC 3 cut(s) 450, 743, 1310
PspPI GGNCC 4 cut(s) 28, 541, 995, 1013
PspPPI RGGWCCY 2 cut(s) 995, 1013
PsrI GAACNNNNNNTAC 2 cut(s) 462, 494
PstNI CAGNNNCTG 1 cut(s) 108
PsuI RGATCY 1 cut(s) 448
PvuII CAGCTG 1 cut(s) 858
RsaI GTAC 2 cut(s) 509, 649
RsaNI GTAC 2 cut(s) 508, 648
RseI CAYNNNNRTG 1 cut(s) 247
SaqAI TTAA 1 cut(s) 1191
SatI GCNGC 3 cut(s) 103, 859, 1068
Sau3AI GATC 5 cut(s) 448, 775, 1040, 1171, 1237
Sau96I GGNCC 4 cut(s) 28, 541, 995, 1013
SchI GAGTC 2 cut(s) 136, 302
ScrFI CCNGG 6 cut(s) 26, 750, 851, 999, 1095, 1353
SduI GDGCHC 2 cut(s) 329, 389
SexAI ACCWGGT 1 cut(s) 1093
SfaNI GCATC 4 cut(s) 214, 559, 663, 855
SinI GGWCC 2 cut(s) 995, 1013
SmiMI CAYNNNNRTG 1 cut(s) 247
SmlI CTYRAG 1 cut(s) 1328
SmoI CTYRAG 1 cut(s) 1328
SnaBI TACGTA 1 cut(s) 651
SsiI CCGC 4 cut(s) 93, 1154, 1306, 1312
SspI AATATT 1 cut(s) 871
SspMI CTAG 4 cut(s) 545, 663, 1055, 1269
StyD4I CCNGG 6 cut(s) 24, 748, 849, 997, 1093, 1351
StyI CCWWGG 2 cut(s) 219, 662
TaaI ACNGT 4 cut(s) 930, 940, 1280, 1395
TaiI ACGT 6 cut(s) 392, 619, 653, 691, 1030, 1077
TaqI TCGA 4 cut(s) 130, 300, 594, 1118
TatI WGTACW 1 cut(s) 507
TfiI GAWTC 2 cut(s) 437, 1359
Tru1I TTAA 1 cut(s) 1191
Tru9I TTAA 1 cut(s) 1191
TscAI CASTG 3 cut(s) 187, 254, 732
TseFI GTSAC 1 cut(s) 691
TseI GCWGC 3 cut(s) 102, 858, 1067
Tsp45I GTSAC 1 cut(s) 691
TspDTI ATGAA 3 cut(s) 387, 780, 828
TspGWI ACGGA 2 cut(s) 305, 1393
TspRI CASTG 3 cut(s) 187, 254, 732
Van91I CCANNNNNTGG 2 cut(s) 76, 253
VneI GTGCAC 2 cut(s) 325, 385
VpaK11BI GGWCC 2 cut(s) 995, 1013
XapI RAATTY 3 cut(s) 72, 132, 1286
XbaI TCTAGA 1 cut(s) 1054
XmaJI CCTAGG 1 cut(s) 662
XmiI GTMKAC 1 cut(s) 603
XmnI GAANNNNTTC 1 cut(s) 1114
XspI CTAG 4 cut(s) 545, 663, 1055, 1269
Zsp2I ATGCAT 1 cut(s) 848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.