MD16G1035200.v1.1

fructose-bisphosphate aldolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015859
Physical Location & Seq
Reverse (-)
2516607 .. 2519829
3223 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1035200.v1.1.491

Sequence Viewer

Length: 1182 bp
ATGGCCTGTTCCAGCTTAGCGAAGCTTAATGCGGCGTCGTCTCAGTGGATCGGACAGCAGTCCTTCACTCAGCGTCCTGGATCGTCGACTAGCCTCGCTACTCGCCGAGTCGCCGTCCCGATCCGTGCCAAGGCCTACACCGACGAACTCGTCCTAACCGCCAAAACTATTGCATCCCCTGGTCGTGGTATACTCGCTATTGATGAATCAAATGCAACTTGTGGAAAGAGGTTAGACTCGATTGGGTTGGACAATACCGAGGTTAACCGACAGGCTTACAGGCAACTTTTGTTGACCACCCCTGGCCTTGGCGAATACATTTCAGGTGCCATTCTTTTTGAGGAAACACTTTACCAGTCTACAACCGATGGGAAGAAATTTGTGGATGTTTTGTGCGACCAGAAAATTGTACCTGGAATCAAAGTTGATAAGGGTTTGGTTCCTCTCCCCGGGTCTAACAATGAATCTTGGTGCCAAGGCTTAGATGGATTGGCTTCACGTTCTGCGGAGTACTATAAGCAAGGTGCTCGTTTTGCTAAATGGCGTACAGTTGTTAGCATTCCTTGTGGTCCTTCTGCTCTAGCTGTTAAGGAAGCTGCATGGGGACTTGCTCGTTATGCTGCCATTTCTCAGGACAATGGTCTGGTGCCCATTGTAGAGCCTGAGATTCTTCTCGACGGGGATCACCCAATCGAAAGGACACTTGAAGTTGCAGAGAAGGTCTGGGCAGAAGTATTCTATTACTTGGCCGAAAACAATGTGGTGTTCGAAGGAATCCTTCTTAAGCCTAGCATGGTTACCCCAGGGGCTGAACACAAGGAAAAGGCTACTCCAGAAGCCATTGCAAAGGCTACCCTCACAGTGCTTAAAAGGAGAGTTCCTCCTGCGGTGCCAGGAATCATGTTTTTGTCAGGAGGACAATCTGAAGCAGAAGCAACCCTCAACCTTCATGCAATGAACCAAAGCCCCAACCCCTGGCATGTATCCTTCTCGTACGCACGTGCCCTGCAGAATTCTGTGCTTAAGGCATGGCAAGGACGTCCTGAGAATGTGGAGGCCGCACAAAAGGCACTTTTGGTGCGAGCAAAGGCAAACTCCTTGGCTCAGCTAGGAAAGTACTCTGCCGCGGGTGAGGACGAGGAAGCCAAGAAGGGTATGTTCGTCAAGGGCTATACATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004332 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005975 GO:0005996 GO:0006006 GO:0006082 GO:0006090 GO:0006091 GO:0006094 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009534 GO:0009536 GO:0009570 GO:0009579 GO:0009987 GO:0010035 GO:0010038 GO:0010287 GO:0016051 GO:0016052 GO:0016053 GO:0016310 GO:0016829 GO:0016830 GO:0016832 GO:0017144 GO:0018130 GO:0019318 GO:0019319 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0031976 GO:0031984 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046364 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046939 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00680 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00030 map00051 map00680 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
Glycolytic

Protein Analysis

394

Amino Acids

42.48

Weight (kDa)

8.43

Isoelectric Point (pI)

40.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glycolytic PF00274 49 - 393 7.3e-160 Fructose-bisphosphate aldolase class-I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015859)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01140
fragaria_vesca FvH4_4g26450 FvH4_4g26450
malus_domestica MD13G1033400.v1.1 MD16G1035200.v1.1
prunus_persica Prupe.1G319500_v2.0.a1
pyrus_communis pycom13g02880 pycom16g03000
rosa_chinensis RchiOBHm_Chr4g0434081
rosa_laevigata RLG00000006702
rosa_multiflora Rmu_sc0000352.1_g000030
rosa_roxburghii Rroxscaffold_5G00375490
rosa_rugosa Rorug04G0273500
rosa_samantha Rh4AG329100
rosa_wichuraiana Rw4G028530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 149
AatII GACGTC 1 cut(s) 1042
AccB1I GGYRCC 4 cut(s) 326, 471, 646, 889
AccB7I CCANNNNNTGG 1 cut(s) 975
AccI GTMKAC 3 cut(s) 86, 190, 359
AccII CGCG 1 cut(s) 1127
AciI CCGC 7 cut(s) 32, 159, 506, 887, 1059, 1125, 1127
AclWI GGATC 4 cut(s) 56, 88, 115, 690
AcoI YGGCCR 1 cut(s) 747
AcsI RAATTY 2 cut(s) 377, 1012
AcuI CTGAAG 1 cut(s) 945
AcvI CACGTG 1 cut(s) 1001
AcyI GRCGYC 2 cut(s) 35, 1039
AfaI GTAC 5 cut(s) 411, 512, 547, 995, 1118
AfiI CCNNNNNNNGG 5 cut(s) 130, 185, 308, 449, 975
AflII CTTAAG 2 cut(s) 782, 1022
AgsI TTSAA 1 cut(s) 707
AjnI CCWGG 7 cut(s) 76, 178, 301, 412, 802, 892, 974
AluBI AGCT 5 cut(s) 15, 25, 584, 596, 1108
AluI AGCT 5 cut(s) 15, 25, 584, 596, 1108
Alw21I GWGCWC 1 cut(s) 529
Alw26I GTCTC 1 cut(s) 45
AlwI GGATC 4 cut(s) 56, 88, 115, 690
AlwNI CAGNNNCTG 1 cut(s) 809
Ama87I CYCGRG 1 cut(s) 449
AoxI GGCC 5 cut(s) 3, 132, 304, 747, 1056
ApeKI GCWGC 2 cut(s) 596, 620
ApoI RAATTY 2 cut(s) 377, 1012
AspS9I GGNCC 1 cut(s) 569
AsuC2I CCSGG 2 cut(s) 450, 451
AsuHPI GGTGA 2 cut(s) 677, 1142
AsuII TTCGAA 1 cut(s) 768
AvaI CYCGRG 1 cut(s) 449
AvaII GGWCC 1 cut(s) 569
BaeGI GKGCMC 2 cut(s) 651, 1006
BanI GGYRCC 4 cut(s) 326, 471, 646, 889
BbrPI CACGTG 1 cut(s) 1001
Bbv12I GWGCWC 1 cut(s) 529
BbvI GCAGC 2 cut(s) 583, 607
BccI CCATC 2 cut(s) 362, 479
BceAI ACGGC 1 cut(s) 98
BcgI CGANNNNNNTGC 2 cut(s) 509, 543
BciT130I CCWGG 7 cut(s) 78, 180, 303, 414, 804, 894, 976
BciVI GTATCC 1 cut(s) 994
BcnI CCSGG 2 cut(s) 450, 451
BcoDI GTCTC 1 cut(s) 45
BfaI CTAG 4 cut(s) 90, 581, 789, 1109
BfmI CTRYAG 1 cut(s) 1007
BfrI CTTAAG 2 cut(s) 782, 1022
BfuI GTATCC 1 cut(s) 994
BisI GCNGC 5 cut(s) 33, 597, 621, 1059, 1125
BlpI GCTNAGC 2 cut(s) 16, 1104
BlsI GCNGC 5 cut(s) 34, 598, 622, 1060, 1126
BmcAI AGTACT 2 cut(s) 512, 1118
Bme1390I CCNGG 9 cut(s) 78, 180, 303, 414, 450, 451, 804, 894, 976
Bme18I GGWCC 1 cut(s) 569
BmeT110I CYCGRG 1 cut(s) 449
BmgT120I GGNCC 1 cut(s) 569
BmiI GGNNCC 5 cut(s) 328, 441, 473, 648, 891
BmrFI CCNGG 9 cut(s) 78, 180, 303, 414, 450, 451, 804, 894, 976
BmsI GCATC 1 cut(s) 182
BoxI GACNNNNGTC 2 cut(s) 58, 639
BplI GAGNNNNNCTC 2 cut(s) 865, 897
BpmI CTGGAG 1 cut(s) 816
Bpu1102I GCTNAGC 2 cut(s) 16, 1104
Bpu14I TTCGAA 1 cut(s) 768
BpuMI CCSGG 2 cut(s) 450, 451
BsaAI YACGTR 1 cut(s) 1001
BsaHI GRCGYC 2 cut(s) 35, 1039
Bsc4I CCNNNNNNNGG 5 cut(s) 130, 185, 308, 449, 975
Bse1I ACTGG 1 cut(s) 355
Bse3DI GCAATG 2 cut(s) 840, 960
BseBI CCWGG 7 cut(s) 78, 180, 303, 414, 804, 894, 976
BseGI GGATG 2 cut(s) 173, 391
BseLI CCNNNNNNNGG 5 cut(s) 130, 185, 308, 449, 975
BseMI GCAATG 2 cut(s) 840, 960
BseMII CTCAG 6 cut(s) 56, 83, 644, 654, 1035, 1118
BseNI ACTGG 1 cut(s) 355
BseSI GKGCMC 2 cut(s) 651, 1006
BseXI GCAGC 2 cut(s) 583, 607
Bsh1236I CGCG 1 cut(s) 1127
BshFI GGCC 5 cut(s) 5, 134, 306, 749, 1058
BshNI GGYRCC 4 cut(s) 326, 471, 646, 889
BsiHKAI GWGCWC 1 cut(s) 529
BsiHKCI CYCGRG 1 cut(s) 449
BsiSI CCGG 1 cut(s) 450
BsiWI CGTACG 1 cut(s) 993
BslFI GGGAC 2 cut(s) 101, 618
BslI CCNNNNNNNGG 5 cut(s) 130, 185, 308, 449, 975
BsmAI GTCTC 1 cut(s) 45
BsmBI CGTCTC 1 cut(s) 45
BsmFI GGGAC 2 cut(s) 101, 618
BsmI GAATGC 1 cut(s) 558
BsnI GGCC 5 cut(s) 5, 134, 306, 749, 1058
BsoBI CYCGRG 1 cut(s) 449
Bsp119I TTCGAA 1 cut(s) 768
Bsp1286I GDGCHC 3 cut(s) 529, 651, 1006
Bsp143I GATC 4 cut(s) 48, 80, 120, 682
Bsp1720I GCTNAGC 2 cut(s) 16, 1104
BspACI CCGC 7 cut(s) 32, 159, 506, 887, 1059, 1125, 1127
BspANI GGCC 5 cut(s) 5, 134, 306, 749, 1058
BspCNI CTCAG 6 cut(s) 55, 82, 643, 655, 1036, 1117
BspFNI CGCG 1 cut(s) 1127
BspLI GGNNCC 5 cut(s) 328, 441, 473, 648, 891
BspMAI CTGCAG 1 cut(s) 1011
BspPI GGATC 4 cut(s) 56, 88, 115, 690
BspT104I TTCGAA 1 cut(s) 768
BspT107I GGYRCC 4 cut(s) 326, 471, 646, 889
BspTI CTTAAG 2 cut(s) 782, 1022
BsrDI GCAATG 2 cut(s) 840, 960
BsrI ACTGG 1 cut(s) 355
BssMI GATC 4 cut(s) 48, 80, 120, 682
BssNAI GTATAC 1 cut(s) 191
BssNI GRCGYC 2 cut(s) 35, 1039
BssT1I CCWWGG 4 cut(s) 129, 307, 475, 1098
Bst1107I GTATAC 1 cut(s) 191
Bst2UI CCWGG 7 cut(s) 78, 180, 303, 414, 804, 894, 976
Bst4CI ACNGT 2 cut(s) 550, 862
BstACI GRCGYC 2 cut(s) 35, 1039
BstAFI CTTAAG 2 cut(s) 782, 1022
BstBAI YACGTR 1 cut(s) 1001
BstBI TTCGAA 1 cut(s) 768
BstC8I GCNNGC 1 cut(s) 1083
BstDEI CTNAG 8 cut(s) 16, 42, 69, 481, 630, 663, 1044, 1104
BstDSI CCRYGG 1 cut(s) 1125
BstEII GGTNACC 1 cut(s) 796
BstF5I GGATG 2 cut(s) 173, 391
BstFNI CGCG 1 cut(s) 1127
BstKTI GATC 4 cut(s) 51, 83, 123, 685
BstMAI GTCTC 1 cut(s) 45
BstMBI GATC 4 cut(s) 48, 80, 120, 682
BstMWI GCNNNNNNNGC 3 cut(s) 533, 617, 1067
BstNI CCWGG 7 cut(s) 78, 180, 303, 414, 804, 894, 976
BstNSI RCATGY 1 cut(s) 983
BstPAI GACNNNNGTC 2 cut(s) 58, 639
BstPI GGTNACC 1 cut(s) 796
BstSCI CCNGG 9 cut(s) 76, 178, 301, 412, 448, 449, 802, 892, 974
BstSFI CTRYAG 1 cut(s) 1007
BstSLI GKGCMC 2 cut(s) 651, 1006
BstUI CGCG 1 cut(s) 1127
BstV1I GCAGC 2 cut(s) 583, 607
BstZ17I GTATAC 1 cut(s) 191
BsuI GTATCC 1 cut(s) 994
BsuRI GGCC 5 cut(s) 5, 134, 306, 749, 1058
BtgI CCRYGG 1 cut(s) 1125
BtsCI GGATG 2 cut(s) 173, 391
BtsIMutI CAGTG 2 cut(s) 50, 867
Cac8I GCNNGC 1 cut(s) 1083
CaiI CAGNNNCTG 1 cut(s) 809
Cfr13I GGNCC 1 cut(s) 569
Cfr42I CCGCGG 1 cut(s) 1128
Cfr9I CCCGGG 1 cut(s) 449
CseI GACGC 2 cut(s) 24, 62
Csp6I GTAC 5 cut(s) 410, 511, 546, 994, 1117
CviAII CATG 6 cut(s) 600, 793, 901, 950, 980, 1029
CviQI GTAC 5 cut(s) 410, 511, 546, 994, 1117
DdeI CTNAG 8 cut(s) 16, 42, 69, 481, 630, 663, 1044, 1104
DpnI GATC 4 cut(s) 50, 82, 122, 684
DpnII GATC 4 cut(s) 48, 80, 120, 682
DrdI GACNNNNNNGTC 1 cut(s) 149
DseDI GACNNNNNNGTC 1 cut(s) 149
EaeI YGGCCR 1 cut(s) 747
Eco130I CCWWGG 4 cut(s) 129, 307, 475, 1098
Eco147I AGGCCT 1 cut(s) 134
Eco47I GGWCC 1 cut(s) 569
Eco57I CTGAAG 1 cut(s) 945
Eco72I CACGTG 1 cut(s) 1001
Eco88I CYCGRG 1 cut(s) 449
Eco91I GGTNACC 1 cut(s) 796
EcoO65I GGTNACC 1 cut(s) 796
EcoRI GAATTC 1 cut(s) 1012
EcoRII CCWGG 7 cut(s) 76, 178, 301, 412, 802, 892, 974
EcoT14I CCWWGG 4 cut(s) 129, 307, 475, 1098
ErhI CCWWGG 4 cut(s) 129, 307, 475, 1098
Esp3I CGTCTC 1 cut(s) 45
FaeI CATG 6 cut(s) 603, 796, 904, 953, 983, 1032
FalI AAGNNNNNCTT 2 cut(s) 762, 794
FaqI GGGAC 2 cut(s) 101, 618
FatI CATG 6 cut(s) 599, 792, 900, 949, 979, 1028
FauI CCCGC 1 cut(s) 1120
FblI GTMKAC 3 cut(s) 86, 190, 359
Fnu4HI GCNGC 5 cut(s) 33, 597, 621, 1059, 1125
FokI GGATG 2 cut(s) 160, 398
Fsp4HI GCNGC 5 cut(s) 33, 597, 621, 1059, 1125
FspBI CTAG 4 cut(s) 90, 581, 789, 1109
GluI GCNGC 5 cut(s) 33, 597, 621, 1059, 1125
GsuI CTGGAG 1 cut(s) 816
HaeIII GGCC 5 cut(s) 5, 134, 306, 749, 1058
HapII CCGG 1 cut(s) 450
HgaI GACGC 2 cut(s) 24, 62
Hin1I GRCGYC 2 cut(s) 35, 1039
Hin1II CATG 6 cut(s) 603, 796, 904, 953, 983, 1032
HincII GTYRAC 3 cut(s) 87, 265, 294
HindII GTYRAC 3 cut(s) 87, 265, 294
HindIII AAGCTT 1 cut(s) 23
HinfI GANTC 8 cut(s) 108, 206, 236, 417, 464, 667, 774, 897
HpaI GTTAAC 1 cut(s) 265
HpaII CCGG 1 cut(s) 450
HphI GGTGA 2 cut(s) 677, 1142
Hpy166II GTNNAC 5 cut(s) 87, 191, 265, 294, 360
Hpy188I TCNGA 2 cut(s) 53, 925
Hpy188III TCNNGA 6 cut(s) 118, 632, 674, 833, 912, 1043
Hpy8I GTNNAC 5 cut(s) 87, 191, 265, 294, 360
Hpy99I CGWCG 4 cut(s) 40, 88, 146, 680
HpyAV CCTTC 8 cut(s) 73, 582, 712, 764, 788, 956, 997, 1144
HpyCH4III ACNGT 2 cut(s) 550, 862
HpyCH4IV ACGT 3 cut(s) 499, 1000, 1039
HpyCH4V TGCA 7 cut(s) 173, 215, 599, 713, 845, 953, 1009
HpyF10VI GCNNNNNNNGC 3 cut(s) 533, 617, 1067
HpyF3I CTNAG 8 cut(s) 16, 42, 69, 481, 630, 663, 1044, 1104
HpySE526I ACGT 3 cut(s) 499, 1000, 1039
Hsp92I GRCGYC 2 cut(s) 35, 1039
Hsp92II CATG 6 cut(s) 603, 796, 904, 953, 983, 1032
KspAI GTTAAC 1 cut(s) 265
KspI CCGCGG 1 cut(s) 1128
Kzo9I GATC 4 cut(s) 48, 80, 120, 682
Lsp1109I GCAGC 2 cut(s) 583, 607
LweI GCATC 1 cut(s) 182
MaeI CTAG 4 cut(s) 90, 581, 789, 1109
MaeII ACGT 3 cut(s) 499, 1000, 1039
MaeIII GTNAC 1 cut(s) 796
MalI GATC 4 cut(s) 50, 82, 122, 684
MboI GATC 4 cut(s) 48, 80, 120, 682
MboII GAAGA 2 cut(s) 385, 662
MhlI GDGCHC 3 cut(s) 529, 651, 1006
MluCI AATT 3 cut(s) 377, 405, 1012
MlyI GAGTC 2 cut(s) 117, 230
MmeI TCCRAC 1 cut(s) 228
MseI TTAA 6 cut(s) 27, 264, 588, 783, 867, 1023
MspA1I CMGCKG 1 cut(s) 1127
MspCI CTTAAG 2 cut(s) 782, 1022
MspI CCGG 1 cut(s) 450
MspR9I CCNGG 9 cut(s) 78, 180, 303, 414, 450, 451, 804, 894, 976
Mva1269I GAATGC 1 cut(s) 558
MvaI CCWGG 7 cut(s) 78, 180, 303, 414, 804, 894, 976
MvnI CGCG 1 cut(s) 1127
MwoI GCNNNNNNNGC 3 cut(s) 533, 617, 1067
NciI CCSGG 2 cut(s) 450, 451
NdeII GATC 4 cut(s) 48, 80, 120, 682
NlaIII CATG 6 cut(s) 603, 796, 904, 953, 983, 1032
NlaIV GGNNCC 5 cut(s) 328, 441, 473, 648, 891
NmeAIII GCCGAG 1 cut(s) 131
NspI RCATGY 1 cut(s) 983
NspV TTCGAA 1 cut(s) 768
PasI CCCWGGG 1 cut(s) 803
PceI AGGCCT 1 cut(s) 134
PctI GAATGC 1 cut(s) 558
PfeI GAWTC 6 cut(s) 206, 417, 464, 667, 774, 897
Pfl23II CGTACG 1 cut(s) 993
PflMI CCANNNNNTGG 1 cut(s) 975
PfoI TCCNGGA 1 cut(s) 76
PkrI GCNGC 5 cut(s) 34, 598, 622, 1060, 1126
PleI GAGTC 2 cut(s) 116, 230
PmaCI CACGTG 1 cut(s) 1001
PmlI CACGTG 1 cut(s) 1001
PpsI GAGTC 2 cut(s) 116, 230
Ppu21I YACGTR 1 cut(s) 1001
PshAI GACNNNNGTC 2 cut(s) 58, 639
Psp6I CCWGG 7 cut(s) 76, 178, 301, 412, 802, 892, 974
PspCI CACGTG 1 cut(s) 1001
PspEI GGTNACC 1 cut(s) 796
PspGI CCWGG 7 cut(s) 76, 178, 301, 412, 802, 892, 974
PspLI CGTACG 1 cut(s) 993
PspN4I GGNNCC 5 cut(s) 328, 441, 473, 648, 891
PspPI GGNCC 1 cut(s) 569
PstI CTGCAG 1 cut(s) 1011
PstNI CAGNNNCTG 1 cut(s) 809
RsaI GTAC 5 cut(s) 411, 512, 547, 995, 1118
RsaNI GTAC 5 cut(s) 410, 511, 546, 994, 1117
SacII CCGCGG 1 cut(s) 1128
SalI GTCGAC 1 cut(s) 85
SaqAI TTAA 6 cut(s) 27, 264, 588, 783, 867, 1023
SatI GCNGC 5 cut(s) 33, 597, 621, 1059, 1125
Sau3AI GATC 4 cut(s) 48, 80, 120, 682
Sau96I GGNCC 1 cut(s) 569
ScaI AGTACT 2 cut(s) 512, 1118
SchI GAGTC 2 cut(s) 117, 230
ScrFI CCNGG 9 cut(s) 78, 180, 303, 414, 450, 451, 804, 894, 976
SduI GDGCHC 3 cut(s) 529, 651, 1006
SfaNI GCATC 1 cut(s) 182
SfcI CTRYAG 1 cut(s) 1007
Sfr303I CCGCGG 1 cut(s) 1128
SfuI TTCGAA 1 cut(s) 768
SgrBI CCGCGG 1 cut(s) 1128
SinI GGWCC 1 cut(s) 569
SmaI CCCGGG 1 cut(s) 451
SmlI CTYRAG 2 cut(s) 782, 1022
SmoI CTYRAG 2 cut(s) 782, 1022
Sse9I AATT 3 cut(s) 377, 405, 1012
SseBI AGGCCT 1 cut(s) 134
SsiI CCGC 7 cut(s) 32, 159, 506, 887, 1059, 1125, 1127
SspMI CTAG 4 cut(s) 90, 581, 789, 1109
StuI AGGCCT 1 cut(s) 134
StyD4I CCNGG 9 cut(s) 76, 178, 301, 412, 448, 449, 802, 892, 974
StyI CCWWGG 4 cut(s) 129, 307, 475, 1098
TaaI ACNGT 2 cut(s) 550, 862
TaiI ACGT 3 cut(s) 502, 1003, 1042
TaqI TCGA 5 cut(s) 86, 239, 675, 693, 768
TasI AATT 3 cut(s) 377, 405, 1012
TatI WGTACW 2 cut(s) 510, 1116
TauI GCSGC 3 cut(s) 35, 1061, 1127
TfiI GAWTC 6 cut(s) 206, 417, 464, 667, 774, 897
Tru1I TTAA 6 cut(s) 27, 264, 588, 783, 867, 1023
Tru9I TTAA 6 cut(s) 27, 264, 588, 783, 867, 1023
TscAI CASTG 2 cut(s) 50, 867
TseI GCWGC 2 cut(s) 596, 620
TspDTI ATGAA 4 cut(s) 219, 477, 938, 971
TspGWI ACGGA 1 cut(s) 113
TspMI CCCGGG 1 cut(s) 449
TspRI CASTG 2 cut(s) 50, 867
Van91I CCANNNNNTGG 1 cut(s) 975
Vha464I CTTAAG 2 cut(s) 782, 1022
VpaK11BI GGWCC 1 cut(s) 569
XapI RAATTY 2 cut(s) 377, 1012
XceI RCATGY 1 cut(s) 983
XcmI CCANNNNNNNNNTGG 1 cut(s) 482
XmaI CCCGGG 1 cut(s) 449
XmiI GTMKAC 3 cut(s) 86, 190, 359
XspI CTAG 4 cut(s) 90, 581, 789, 1109
ZraI GACGTC 1 cut(s) 1040
ZrmI AGTACT 2 cut(s) 512, 1118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.