MD16G1043600.v1.1

CPuORF53

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0019702
Physical Location & Seq
Reverse (-)
3060010 .. 3060640
631 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1043600.v1.1.491

Sequence Viewer

Length: 246 bp
ATGTCGGCTAGCTTTGATTTGGTGGTGCTTGGGGCTCCGTCGAAGCTCTGGATTTTAACTGCTGCTTGCTCTGCCGTCTGCTTTATGCTTCTCTGCTTTTTCCGGCAGAGATTCAACCCGGTGATTCGACCTGTTTCGATGATTTTGAGACCAAAAAGGATTTGTTCTGGATTGCAATATTTTGGGGCTTTTCATATAAAAAGATTTTCTTTCCTCTTCTTATTCCTGAGAGTGGATCTAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

82

Amino Acids

9.32

Weight (kDa)

10.45

Isoelectric Point (pI)

61.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019702)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g27490
malus_domestica MD16G1043600.v1.1
prunus_persica Prupe.1G310000_v2.0.a1
rosa_roxburghii Rroxscaffold_5G00376560
rosa_rugosa Rorug04G0284200
rosa_samantha Rh4BG347900
rosa_wichuraiana Rw4G029620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 243
AfiI CCNNNNNNNGG 1 cut(s) 232
AgsI TTSAA 1 cut(s) 115
AluBI AGCT 2 cut(s) 12, 46
AluI AGCT 2 cut(s) 12, 46
Alw26I GTCTC 1 cut(s) 142
AlwI GGATC 1 cut(s) 243
ApeKI GCWGC 1 cut(s) 62
AsuC2I CCSGG 1 cut(s) 119
AsuHPI GGTGA 1 cut(s) 133
AsuNHI GCTAGC 1 cut(s) 8
BanII GRGCYC 1 cut(s) 37
BbvI GCAGC 1 cut(s) 49
BceAI ACGGC 1 cut(s) 59
BcnI CCSGG 1 cut(s) 119
BcoDI GTCTC 1 cut(s) 142
BfaI CTAG 1 cut(s) 9
BisI GCNGC 1 cut(s) 63
BlsI GCNGC 1 cut(s) 64
Bme1390I CCNGG 1 cut(s) 119
BmiI GGNNCC 1 cut(s) 36
BmrFI CCNGG 1 cut(s) 119
BmtI GCTAGC 1 cut(s) 12
BpuMI CCSGG 1 cut(s) 119
BsaI GGTCTC 1 cut(s) 142
Bsc4I CCNNNNNNNGG 1 cut(s) 232
BseLI CCNNNNNNNGG 1 cut(s) 232
BseMII CTCAG 1 cut(s) 218
BseXI GCAGC 1 cut(s) 49
BsiSI CCGG 2 cut(s) 103, 119
BslI CCNNNNNNNGG 1 cut(s) 232
BsmAI GTCTC 1 cut(s) 142
Bso31I GGTCTC 1 cut(s) 142
Bsp1286I GDGCHC 1 cut(s) 37
Bsp143I GATC 1 cut(s) 235
BspCNI CTCAG 1 cut(s) 219
BspLI GGNNCC 1 cut(s) 36
BspOI GCTAGC 1 cut(s) 12
BspPI GGATC 1 cut(s) 243
BspTNI GGTCTC 1 cut(s) 142
BssMI GATC 1 cut(s) 235
Bst6I CTCTTC 1 cut(s) 221
BstC8I GCNNGC 2 cut(s) 10, 67
BstDEI CTNAG 1 cut(s) 227
BstKTI GATC 1 cut(s) 238
BstMAI GTCTC 1 cut(s) 142
BstMBI GATC 1 cut(s) 235
BstMWI GCNNNNNNNGC 1 cut(s) 71
BstSCI CCNGG 1 cut(s) 117
BstV1I GCAGC 1 cut(s) 49
BstX2I RGATCY 1 cut(s) 235
BstYI RGATCY 1 cut(s) 235
Cac8I GCNNGC 2 cut(s) 10, 67
CviJI RGCY 5 cut(s) 8, 12, 35, 46, 188
CviKI_1 RGCY 5 cut(s) 8, 12, 35, 46, 188
DdeI CTNAG 1 cut(s) 227
DpnI GATC 1 cut(s) 237
DpnII GATC 1 cut(s) 235
Eam1104I CTCTTC 1 cut(s) 221
EarI CTCTTC 1 cut(s) 221
Eco24I GRGCYC 1 cut(s) 37
Eco31I GGTCTC 1 cut(s) 142
EcoT38I GRGCYC 1 cut(s) 37
FaiI YATR 3 cut(s) 86, 195, 197
FalI AAGNNNNNCTT 2 cut(s) 193, 225
Fnu4HI GCNGC 1 cut(s) 63
FriOI GRGCYC 1 cut(s) 37
Fsp4HI GCNGC 1 cut(s) 63
FspBI CTAG 1 cut(s) 9
GluI GCNGC 1 cut(s) 63
HapII CCGG 2 cut(s) 103, 119
HinfI GANTC 2 cut(s) 111, 124
HpaII CCGG 2 cut(s) 103, 119
HphI GGTGA 1 cut(s) 133
Hpy188III TCNNGA 3 cut(s) 49, 168, 226
Hpy99I CGWCG 1 cut(s) 43
HpyCH4V TGCA 1 cut(s) 175
HpyF10VI GCNNNNNNNGC 1 cut(s) 71
HpyF3I CTNAG 1 cut(s) 227
Kzo9I GATC 1 cut(s) 235
LmnI GCTCC 1 cut(s) 40
LpnPI CCDG 6 cut(s) 34, 116, 132, 144, 153, 239
Lsp1109I GCAGC 1 cut(s) 49
MaeI CTAG 1 cut(s) 9
MalI GATC 1 cut(s) 237
MboI GATC 1 cut(s) 235
MboII GAAGA 1 cut(s) 208
MflI RGATCY 1 cut(s) 235
MhlI GDGCHC 1 cut(s) 37
MnlI CCTC 1 cut(s) 224
MseI TTAA 1 cut(s) 56
MspI CCGG 2 cut(s) 103, 119
MspR9I CCNGG 1 cut(s) 119
MwoI GCNNNNNNNGC 1 cut(s) 71
NciI CCSGG 1 cut(s) 119
NdeII GATC 1 cut(s) 235
NheI GCTAGC 1 cut(s) 8
NlaIV GGNNCC 1 cut(s) 36
PfeI GAWTC 2 cut(s) 111, 124
PkrI GCNGC 1 cut(s) 64
PspN4I GGNNCC 1 cut(s) 36
PsuI RGATCY 1 cut(s) 235
SaqAI TTAA 1 cut(s) 56
SatI GCNGC 1 cut(s) 63
Sau3AI GATC 1 cut(s) 235
ScrFI CCNGG 1 cut(s) 119
SduI GDGCHC 1 cut(s) 37
SetI ASST 4 cut(s) 14, 48, 133, 245
SspI AATATT 1 cut(s) 179
SspMI CTAG 1 cut(s) 9
StyD4I CCNGG 1 cut(s) 117
TaqI TCGA 3 cut(s) 41, 127, 137
TfiI GAWTC 2 cut(s) 111, 124
Tru1I TTAA 1 cut(s) 56
Tru9I TTAA 1 cut(s) 56
TseI GCWGC 1 cut(s) 62
TspDTI ATGAA 1 cut(s) 182
TspGWI ACGGA 1 cut(s) 27
XspI CTAG 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.