Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0016653

Physical Location & Seq

Genomic Coordinates Reverse (-)

10976308 .. 10979216

Gene Structure 2909 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1142800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1836 bp

ATGTTGCCAAGGATGATTCCCAGATATAATCCAAATGACAGTGAATATGGTTTGAAGCTTCTTGAGGACCTGACAACAAATGCATATGAAGTACAGCAGCGGGTGTTGGAAGAGATAATAGTGAAAAATGCCCAGACCGAATATCTGAAGGGCTTTCTCAATGGCCACCATGACAAGAAGGATTTCAAGAACAAAGTTCCTGTTGTGAATTATGAAGATGTCAAGCCTTACATCGAGCGAATTGCCAATGGAGAGACGTCTGAGATCATCTCGGCTCAAAAAATCACTGAGCTCCTCACCAGCTCGGGAACTTCGGGAGGACAGCCGAAGATGATGCCTTCAACCGTTGAAGACTTGGACAGGAAGACATTCTTTTATAATCTCCTTGTGCCTGTGATGAACAAGTATGTGGATGGCTTGGACCAAGGGAAAGGAATGTACCTCTTGTTTATCAAACCGGAAATTAGTACTCCTTCCGGATTAGTGGCAAGGCCTGTCCTGACCAGCTACTACAAGAGCAGTAACTTCCGCAACCGGCCTTTCAACCGGTTTAACATCTACACAAGCCCGGATGAGACCATCTTGTGCTCAGACAGCAAGCAGAGTATGTACTGCCAGTTACTCTGTGGTTTAGTACAGAGGGATGAGGTCCTGAGGGTCGGTGCAGTTTTTGCGTCCGCCTTCCTCCGGGCCATCAAATTCTTAGAGGACCACTGGGAGGAGTTGTGCTCAAACATAAGCTCAGGTATTGTCAGTGCCTGGATCACCGACTCCAGTTGTCGAAACGCGGTGTCTTTAGTCCTTAGCAAACCGAATCCAGATTTGGGCGATTTGATCAGGCACGAATTTAGTAACAAGTCAATGGAAGGGATAGTTAAGAGGCTTTGGCCAAGAACAAAGTACATTGATGTTATAGTCACAGGGTCCATGGCTCAGTATATCCCAACTCTTGAATTCTATTCTGGTGGACTGCCTTTGATTTCAACAATGTATGCTTCTTCTGAATGCTATTTCGGGATCAATTTCAACCCGCTAAGCAAGCCGCATGATGTTGCTTACACCCTCCTTCCGAACATGGCCTACTTCGAGTTCCTACCCGTGGAGAAACACCACGAAGAGGTAGCCCCGTGCAACGGTGTTTCTGGTCAAGATTGCGCAAACGCAGATGAGGAGGAAAATTTAAGAACGGTTGATCTAGTGGATGTGAAGCTTGGTCACTATTATGAACTTGTTGTCACAACTTTTACAGGGCTATACAGGTACAGAGTTGGTGACATTCTAATGGTGACTGGCTTCCACAACAAAGCTCCTCAGTTCAGATTCATGCACAGGAGAAATGTGGTTCTGAGCATCGACACGGACAAAACCAATGAAGAAGACCTTCTAAACGCCGTCACGCAAGCAAAGCTCCTGCTCGAGCCCCTCGGCTTCCTCCTCATCGAGTACACCAGCTACGCGGACACCTCCTCAATCCCAGGCCACTATGTGCTGTTCTGGGAGCTCAAGACCAAAGAAAACAACGACACGATAGAACTGGAGACCATCATTATGGAGCAATGCTGCTCCACAGTGGAACAATCCCTGGACTCGGTGTACCGGAGGTGCAGGAGGAAGGACAATTCCATAGGACCACTGGAGATCCGGATTGTGCAGCATGGGACTTTTGATGCACTCATGGATTTCTCTGTGTCCCAGGGATCTTCTGTGAACCAGTACAAAACTCCCAGGTGCATCCAGTCAGAGGAGGCCATTAGGATTCTGGATTCCAGGGTGGTGGGAAGATTCTTCAGTAAATCAACTCCTCTATGGGAGCCATTTAGAATAGAGACTCAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0010252 GO:0010279 GO:0016874 GO:0016879 GO:0016881 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048878 GO:0050896 GO:0065007 GO:0065008

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

GH3

Protein Analysis

612

Amino Acids

69.66

Weight (kDa)

5.49

Isoelectric Point (pI)

43.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GH3	PF03321	18 - 326	7.7e-119	GH3 family N-terminal domain
GH3_M	PF23571	353 - 444	1.5e-29	GH3 family central domain
GH3_C	PF23572	459 - 582	2.2e-39	GH3 family C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016653)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G28130
fragaria_vesca	FvH4_4g22430
malus_domestica	MD13G1132300.v1.1 MD16G1142800.v1.1
prunus_persica	Prupe.1G213200_v2.0.a1 Prupe.1G213200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0428671
rosa_laevigata	RLG00000007136
rosa_multiflora	Rmu_sc0004421.1_g000008
rosa_roxburghii	Rroxscaffold_5G00370340
rosa_rugosa	Rorug04G0227000
rosa_samantha	Rh4AG284400 Rh4BG290300
rosa_wichuraiana	Rw4G024700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	378
AatII	GACGTC	1 cut(s)	260
Acc16I	TGCGCA	1 cut(s)	1156
AccII	CGCG	2 cut(s)	788, 1457
AccIII	TCCGGA	2 cut(s)	476, 1641
AciI	CCGC	7 cut(s)	100, 529, 678, 788, 1031, 1043, 1457
AclWI	GGATC	4 cut(s)	770, 1025, 1633, 1705
AcoI	YGGCCR	2 cut(s)	163, 887
AcsI	RAATTY	4 cut(s)	698, 845, 953, 1177
AcuI	CTGAAG	2 cut(s)	167, 1771
AcyI	GRCGYC	1 cut(s)	257
AdeI	CACNNNGTG	1 cut(s)	1486
AfiI	CCNNNNNNNGG	8 cut(s)	687, 718, 824, 1099, 1117, 1133, 1588, 1741
AgeI	ACCGGT	1 cut(s)	546
AgsI	TTSAA	8 cut(s)	55, 187, 342, 350, 544, 953, 984, 1027
AjnI	CCWGG	6 cut(s)	758, 1474, 1581, 1692, 1724, 1766
AjuI	GAANNNNNNNTTGG	2 cut(s)	806, 838
Alw21I	GWGCWC	4 cut(s)	294, 590, 731, 1503
Alw26I	GTCTC	4 cut(s)	248, 569, 1532, 1820
AlwI	GGATC	4 cut(s)	770, 1025, 1633, 1705
AlwNI	CAGNNNCTG	1 cut(s)	759
Ama87I	CYCGRG	2 cut(s)	304, 1415
Aor13HI	TCCGGA	2 cut(s)	476, 1641
AoxI	GGCC	8 cut(s)	163, 491, 536, 690, 887, 1077, 1477, 1746
ApeKI	GCWGC	3 cut(s)	97, 1560, 1651
ApoI	RAATTY	4 cut(s)	698, 845, 953, 1177
AsiGI	ACCGGT	1 cut(s)	546
Asp700I	GAANNNNTTC	3 cut(s)	182, 368, 1380
AspLEI	GCGC	1 cut(s)	1157
AspS9I	GGNCC	7 cut(s)	67, 421, 649, 690, 709, 924, 1628
AsuC2I	CCSGG	2 cut(s)	569, 689
AsuHPI	GGTGA	4 cut(s)	289, 757, 1283, 1297
AvaI	CYCGRG	2 cut(s)	304, 1415
AvaII	GGWCC	6 cut(s)	67, 421, 649, 709, 924, 1628
AxyI	CCTNAGG	1 cut(s)	653
BalI	TGGCCA	2 cut(s)	165, 889
BanII	GRGCYC	3 cut(s)	294, 1422, 1503
BbsI	GAAGAC	3 cut(s)	357, 371, 1383
Bbv12I	GWGCWC	4 cut(s)	294, 590, 731, 1503
BbvI	GCAGC	3 cut(s)	109, 1547, 1663
BccI	CCATC	4 cut(s)	407, 587, 701, 1550
BceAI	ACGGC	1 cut(s)	1376
BcgI	CGANNNNNNTGC	4 cut(s)	224, 258, 316, 350
BciT130I	CCWGG	6 cut(s)	760, 1476, 1583, 1694, 1726, 1768
BclI	TGATCA	1 cut(s)	834
BcnI	CCSGG	2 cut(s)	569, 689
BcoDI	GTCTC	4 cut(s)	248, 569, 1532, 1820
BfaI	CTAG	1 cut(s)	1196
BisI	GCNGC	4 cut(s)	98, 1043, 1561, 1652
BlpI	GCTNAGC	1 cut(s)	1034
BlsI	GCNGC	4 cut(s)	99, 1044, 1562, 1653
BmcAI	AGTACT	1 cut(s)	469
Bme1390I	CCNGG	8 cut(s)	569, 689, 760, 1476, 1583, 1694, 1726, 1768
Bme18I	GGWCC	6 cut(s)	67, 421, 649, 709, 924, 1628
BmeT110I	CYCGRG	2 cut(s)	304, 1415
BmgT120I	GGNCC	7 cut(s)	67, 421, 649, 690, 709, 924, 1628
BmiI	GGNNCC	2 cut(s)	925, 1812
BmrFI	CCNGG	8 cut(s)	569, 689, 760, 1476, 1583, 1694, 1726, 1768
BmrI	ACTGGG	1 cut(s)	724
BmsI	GCATC	4 cut(s)	324, 1359, 1657, 1740
BmuI	ACTGGG	1 cut(s)	724
BpiI	GAAGAC	3 cut(s)	357, 371, 1383
BplI	GAGNNNNNCTC	4 cut(s)	254, 286, 713, 745
BpmI	CTGGAG	3 cut(s)	757, 1556, 1655
Bpu10I	CCTNAGC	2 cut(s)	742, 803
Bpu1102I	GCTNAGC	1 cut(s)	1034
BpuEI	CTTGAG	2 cut(s)	83, 1487
BpuMI	CCSGG	2 cut(s)	569, 689
BsaHI	GRCGYC	1 cut(s)	257
BsaI	GGTCTC	2 cut(s)	569, 1532
BsaWI	WCCGGW	5 cut(s)	457, 476, 546, 1596, 1641
BsaXI	ACNNNNNCTCC	2 cut(s)	710, 740
Bsc4I	CCNNNNNNNGG	8 cut(s)	687, 718, 824, 1099, 1117, 1133, 1588, 1741
Bse118I	RCCGGY	2 cut(s)	534, 546
Bse1I	ACTGG	8 cut(s)	616, 719, 774, 1294, 1539, 1638, 1711, 1735
Bse21I	CCTNAGG	1 cut(s)	653
Bse3DI	GCAATG	1 cut(s)	1562
BseAI	TCCGGA	2 cut(s)	476, 1641
BseBI	CCWGG	6 cut(s)	760, 1476, 1583, 1694, 1726, 1768
BseGI	GGATG	6 cut(s)	18, 418, 577, 649, 1207, 1731
BseLI	CCNNNNNNNGG	8 cut(s)	687, 718, 824, 1099, 1117, 1133, 1588, 1741
BseMI	GCAATG	1 cut(s)	1562
BseMII	CTCAG	8 cut(s)	252, 279, 603, 644, 756, 947, 1325, 1337
BseNI	ACTGG	8 cut(s)	616, 719, 774, 1294, 1539, 1638, 1711, 1735
BseRI	GAGGAG	8 cut(s)	284, 734, 1184, 1299, 1424, 1456, 1757, 1791
BseXI	GCAGC	3 cut(s)	109, 1547, 1663
BsgI	GTGCAG	3 cut(s)	684, 1624, 1670
Bsh1236I	CGCG	2 cut(s)	788, 1457
BshFI	GGCC	8 cut(s)	165, 493, 538, 692, 889, 1079, 1479, 1748
BshTI	ACCGGT	1 cut(s)	546
BsiHKAI	GWGCWC	4 cut(s)	294, 590, 731, 1503
BsiHKCI	CYCGRG	2 cut(s)	304, 1415
BsiSI	CCGG	8 cut(s)	458, 477, 535, 547, 569, 688, 1597, 1642
BslFI	GGGAC	2 cut(s)	1672, 1675
BslI	CCNNNNNNNGG	8 cut(s)	687, 718, 824, 1099, 1117, 1133, 1588, 1741
BsmAI	GTCTC	4 cut(s)	248, 569, 1532, 1820
BsmBI	CGTCTC	1 cut(s)	248
BsmFI	GGGAC	2 cut(s)	1672, 1675
BsmI	GAATGC	1 cut(s)	1010
BsnI	GGCC	8 cut(s)	165, 493, 538, 692, 889, 1079, 1479, 1748
Bso31I	GGTCTC	2 cut(s)	569, 1532
BsoBI	CYCGRG	2 cut(s)	304, 1415
Bsp1286I	GDGCHC	5 cut(s)	294, 590, 731, 1422, 1503
Bsp13I	TCCGGA	2 cut(s)	476, 1641
Bsp143I	GATC	7 cut(s)	264, 762, 834, 1017, 1192, 1638, 1697
Bsp1720I	GCTNAGC	1 cut(s)	1034
Bsp19I	CCATGG	1 cut(s)	927
BspACI	CCGC	7 cut(s)	100, 529, 678, 788, 1031, 1043, 1457
BspANI	GGCC	8 cut(s)	165, 493, 538, 692, 889, 1079, 1479, 1748
BspCNI	CTCAG	8 cut(s)	253, 280, 602, 645, 755, 946, 1324, 1338
BspEI	TCCGGA	2 cut(s)	476, 1641
BspFNI	CGCG	2 cut(s)	788, 1457
BspLI	GGNNCC	2 cut(s)	925, 1812
BspPI	GGATC	4 cut(s)	770, 1025, 1633, 1705
BspTNI	GGTCTC	2 cut(s)	569, 1532
BsrDI	GCAATG	1 cut(s)	1562
BsrFI	RCCGGY	2 cut(s)	534, 546
BsrI	ACTGG	8 cut(s)	616, 719, 774, 1294, 1539, 1638, 1711, 1735
BssAI	RCCGGY	2 cut(s)	534, 546
BssMI	GATC	7 cut(s)	264, 762, 834, 1017, 1192, 1638, 1697
BssNI	GRCGYC	1 cut(s)	257
BssT1I	CCWWGG	3 cut(s)	8, 424, 927
Bst2UI	CCWGG	6 cut(s)	760, 1476, 1583, 1694, 1726, 1768
Bst4CI	ACNGT	5 cut(s)	41, 346, 1136, 1189, 1570
Bst6I	CTCTTC	2 cut(s)	105, 1110
BstACI	GRCGYC	1 cut(s)	257
BstAPI	GCANNNNNTGC	1 cut(s)	671
BstC8I	GCNNGC	3 cut(s)	599, 1040, 1401
BstDSI	CCRYGG	2 cut(s)	927, 1098
BstF5I	GGATG	6 cut(s)	18, 418, 577, 649, 1207, 1731
BstFNI	CGCG	2 cut(s)	788, 1457
BstHHI	GCGC	1 cut(s)	1157
BstKTI	GATC	7 cut(s)	267, 765, 837, 1020, 1195, 1641, 1700
BstMAI	GTCTC	4 cut(s)	248, 569, 1532, 1820
BstMBI	GATC	7 cut(s)	264, 762, 834, 1017, 1192, 1638, 1697
BstMWI	GCNNNNNNNGC	4 cut(s)	594, 671, 1039, 1405
BstNI	CCWGG	6 cut(s)	760, 1476, 1583, 1694, 1726, 1768
BstSCI	CCNGG	8 cut(s)	567, 687, 758, 1474, 1581, 1692, 1724, 1766
BstUI	CGCG	2 cut(s)	788, 1457
BstV1I	GCAGC	3 cut(s)	109, 1547, 1663
BstV2I	GAAGAC	3 cut(s)	357, 371, 1383
BstX2I	RGATCY	2 cut(s)	1638, 1697
BstXI	CCANNNNNNTGG	2 cut(s)	1549, 1774
BstYI	RGATCY	2 cut(s)	1638, 1697
Bsu36I	CCTNAGG	1 cut(s)	653
BsuRI	GGCC	8 cut(s)	165, 493, 538, 692, 889, 1079, 1479, 1748
BtgI	CCRYGG	2 cut(s)	927, 1098
BtsCI	GGATG	6 cut(s)	18, 418, 577, 649, 1207, 1731
BtsIMutI	CAGTG	6 cut(s)	46, 285, 712, 760, 1575, 1631
Cac8I	GCNNGC	3 cut(s)	599, 1040, 1401
CaiI	CAGNNNCTG	1 cut(s)	759
CfoI	GCGC	1 cut(s)	1157
Cfr10I	RCCGGY	2 cut(s)	534, 546
Cfr13I	GGNCC	7 cut(s)	67, 421, 649, 690, 709, 924, 1628
CseI	GACGC	1 cut(s)	663
CspAI	ACCGGT	1 cut(s)	546
CviAII	CATG	7 cut(s)	170, 928, 1046, 1075, 1324, 1655, 1675
DpnI	GATC	7 cut(s)	266, 764, 836, 1019, 1194, 1640, 1699
DpnII	GATC	7 cut(s)	264, 762, 834, 1017, 1192, 1638, 1697
DraIII	CACNNNGTG	1 cut(s)	1486
EaeI	YGGCCR	2 cut(s)	163, 887
Eam1104I	CTCTTC	2 cut(s)	105, 1110
EarI	CTCTTC	2 cut(s)	105, 1110
EciI	GGCGGA	1 cut(s)	667
Ecl136II	GAGCTC	2 cut(s)	292, 1501
Eco130I	CCWWGG	3 cut(s)	8, 424, 927
Eco147I	AGGCCT	1 cut(s)	493
Eco24I	GRGCYC	3 cut(s)	294, 1422, 1503
Eco31I	GGTCTC	2 cut(s)	569, 1532
Eco47I	GGWCC	6 cut(s)	67, 421, 649, 709, 924, 1628
Eco53kI	GAGCTC	2 cut(s)	292, 1501
Eco57I	CTGAAG	2 cut(s)	167, 1771
Eco81I	CCTNAGG	1 cut(s)	653
Eco88I	CYCGRG	2 cut(s)	304, 1415
EcoICRI	GAGCTC	2 cut(s)	292, 1501
EcoO109I	RGGNCCY	2 cut(s)	67, 649
EcoRI	GAATTC	1 cut(s)	953
EcoRII	CCWGG	6 cut(s)	758, 1474, 1581, 1692, 1724, 1766
EcoT14I	CCWWGG	3 cut(s)	8, 424, 927
EcoT22I	ATGCAT	1 cut(s)	85
EcoT38I	GRGCYC	3 cut(s)	294, 1422, 1503
ErhI	CCWWGG	3 cut(s)	8, 424, 927
Esp3I	CGTCTC	1 cut(s)	248
FaeI	CATG	7 cut(s)	173, 931, 1049, 1078, 1327, 1658, 1678
FalI	AAGNNNNNCTT	4 cut(s)	356, 388, 1365, 1397
FaqI	GGGAC	2 cut(s)	1672, 1675
FatI	CATG	7 cut(s)	169, 927, 1045, 1074, 1323, 1654, 1674
FauI	CCCGC	2 cut(s)	93, 1038
FauNDI	CATATG	1 cut(s)	85
FbaI	TGATCA	1 cut(s)	834
Fnu4HI	GCNGC	4 cut(s)	98, 1043, 1561, 1652
FokI	GGATG	6 cut(s)	25, 425, 584, 656, 1214, 1718
FriOI	GRGCYC	3 cut(s)	294, 1422, 1503
Fsp4HI	GCNGC	4 cut(s)	98, 1043, 1561, 1652
FspBI	CTAG	1 cut(s)	1196
FspI	TGCGCA	1 cut(s)	1156
GlaI	GCGC	1 cut(s)	1156
GluI	GCNGC	4 cut(s)	98, 1043, 1561, 1652
GsuI	CTGGAG	3 cut(s)	757, 1556, 1655
HaeIII	GGCC	8 cut(s)	165, 493, 538, 692, 889, 1079, 1479, 1748
HapII	CCGG	8 cut(s)	458, 477, 535, 547, 569, 688, 1597, 1642
HgaI	GACGC	1 cut(s)	663
HhaI	GCGC	1 cut(s)	1157
Hin1I	GRCGYC	1 cut(s)	257
Hin1II	CATG	7 cut(s)	173, 931, 1049, 1078, 1327, 1658, 1678
Hin6I	GCGC	1 cut(s)	1155
HinP1I	GCGC	1 cut(s)	1155
HindIII	AAGCTT	2 cut(s)	56, 1208
HinfI	GANTC	9 cut(s)	16, 770, 814, 1320, 1586, 1756, 1763, 1782, 1828
HpaII	CCGG	8 cut(s)	458, 477, 535, 547, 569, 688, 1597, 1642
HphI	GGTGA	4 cut(s)	289, 757, 1283, 1297
Hpy166II	GTNNAC	4 cut(s)	968, 1446, 1594, 1708
Hpy188I	TCNGA	8 cut(s)	147, 262, 592, 1003, 1071, 1319, 1347, 1741
Hpy8I	GTNNAC	4 cut(s)	968, 1446, 1594, 1708
HpyAV	CCTTC	9 cut(s)	142, 172, 348, 483, 691, 860, 1076, 1391, 1606
HpyCH4III	ACNGT	5 cut(s)	41, 346, 1136, 1189, 1570
HpyCH4IV	ACGT	1 cut(s)	257
HpyCH4V	TGCA	8 cut(s)	83, 665, 1131, 1327, 1605, 1651, 1670, 1731
HpyF10VI	GCNNNNNNNGC	4 cut(s)	594, 671, 1039, 1405
HpySE526I	ACGT	1 cut(s)	257
Hsp92I	GRCGYC	1 cut(s)	257
Hsp92II	CATG	7 cut(s)	173, 931, 1049, 1078, 1327, 1658, 1678
HspAI	GCGC	1 cut(s)	1155
Kpn2I	TCCGGA	2 cut(s)	476, 1641
Ksp22I	TGATCA	1 cut(s)	834
Kzo9I	GATC	7 cut(s)	264, 762, 834, 1017, 1192, 1638, 1697
LmnI	GCTCC	7 cut(s)	297, 1312, 1413, 1498, 1552, 1568, 1810
Lsp1109I	GCAGC	3 cut(s)	109, 1547, 1663
LweI	GCATC	4 cut(s)	324, 1359, 1657, 1740
MaeI	CTAG	1 cut(s)	1196
MaeII	ACGT	1 cut(s)	257
MaeIII	GTNAC	9 cut(s)	521, 618, 851, 916, 1214, 1234, 1271, 1285, 1393
MalI	GATC	7 cut(s)	266, 764, 836, 1019, 1194, 1640, 1699
MboI	GATC	7 cut(s)	264, 762, 834, 1017, 1192, 1638, 1697
MflI	RGATCY	2 cut(s)	1638, 1697
MhlI	GDGCHC	5 cut(s)	294, 590, 731, 1422, 1503
MlsI	TGGCCA	2 cut(s)	165, 889
MluCI	AATT	9 cut(s)	208, 240, 462, 698, 845, 953, 1021, 1177, 1618
MluNI	TGGCCA	2 cut(s)	165, 889
MlyI	GAGTC	3 cut(s)	764, 1580, 1822
MmeI	TCCRAC	1 cut(s)	87
Mox20I	TGGCCA	2 cut(s)	165, 889
Mph1103I	ATGCAT	1 cut(s)	85
MroI	TCCGGA	2 cut(s)	476, 1641
MroXI	GAANNNNTTC	3 cut(s)	182, 368, 1380
MscI	TGGCCA	2 cut(s)	165, 889
MseI	TTAA	3 cut(s)	552, 876, 1181
MslI	CAYNNNNRTG	3 cut(s)	1221, 1280, 1547
Msp20I	TGGCCA	2 cut(s)	165, 889
MspA1I	CMGCKG	1 cut(s)	100
MspI	CCGG	8 cut(s)	458, 477, 535, 547, 569, 688, 1597, 1642
MspR9I	CCNGG	8 cut(s)	569, 689, 760, 1476, 1583, 1694, 1726, 1768
Mva1269I	GAATGC	1 cut(s)	1010
MvaI	CCWGG	6 cut(s)	760, 1476, 1583, 1694, 1726, 1768
MvnI	CGCG	2 cut(s)	788, 1457
MwoI	GCNNNNNNNGC	4 cut(s)	594, 671, 1039, 1405
NciI	CCSGG	2 cut(s)	569, 689
NcoI	CCATGG	1 cut(s)	927
NdeI	CATATG	1 cut(s)	85
NdeII	GATC	7 cut(s)	264, 762, 834, 1017, 1192, 1638, 1697
NlaIII	CATG	7 cut(s)	173, 931, 1049, 1078, 1327, 1658, 1678
NlaIV	GGNNCC	2 cut(s)	925, 1812
NmeAIII	GCCGAG	2 cut(s)	251, 1404
NmuCI	GTSAC	6 cut(s)	916, 1214, 1234, 1271, 1285, 1393
NsbI	TGCGCA	1 cut(s)	1156
NsiI	ATGCAT	1 cut(s)	85
PaeR7I	CTCGAG	1 cut(s)	1415
PasI	CCCWGGG	1 cut(s)	1693
PceI	AGGCCT	1 cut(s)	493
PctI	GAATGC	1 cut(s)	1010
PdmI	GAANNNNTTC	3 cut(s)	182, 368, 1380
PfeI	GAWTC	6 cut(s)	16, 814, 1320, 1756, 1763, 1782
PinAI	ACCGGT	1 cut(s)	546
PkrI	GCNGC	4 cut(s)	99, 1044, 1562, 1653
PleI	GAGTC	3 cut(s)	764, 1580, 1822
PpsI	GAGTC	3 cut(s)	764, 1580, 1822
PpuMI	RGGWCCY	2 cut(s)	67, 649
PsiI	TTATAA	1 cut(s)	378
Psp124BI	GAGCTC	2 cut(s)	294, 1503
Psp5II	RGGWCCY	2 cut(s)	67, 649
Psp6I	CCWGG	6 cut(s)	758, 1474, 1581, 1692, 1724, 1766
PspGI	CCWGG	6 cut(s)	758, 1474, 1581, 1692, 1724, 1766
PspN4I	GGNNCC	2 cut(s)	925, 1812
PspPI	GGNCC	7 cut(s)	67, 421, 649, 690, 709, 924, 1628
PspPPI	RGGWCCY	2 cut(s)	67, 649
PspXI	VCTCGAGB	1 cut(s)	1415
PsrI	GAACNNNNNNTAC	2 cut(s)	1064, 1096
PstNI	CAGNNNCTG	1 cut(s)	759
PsuI	RGATCY	2 cut(s)	1638, 1697
RseI	CAYNNNNRTG	3 cut(s)	1221, 1280, 1547
SacI	GAGCTC	2 cut(s)	294, 1503
SaqAI	TTAA	3 cut(s)	552, 876, 1181
SatI	GCNGC	4 cut(s)	98, 1043, 1561, 1652
Sau3AI	GATC	7 cut(s)	264, 762, 834, 1017, 1192, 1638, 1697
Sau96I	GGNCC	7 cut(s)	67, 421, 649, 690, 709, 924, 1628
ScaI	AGTACT	1 cut(s)	469
SchI	GAGTC	3 cut(s)	764, 1580, 1822
ScrFI	CCNGG	8 cut(s)	569, 689, 760, 1476, 1583, 1694, 1726, 1768
SduI	GDGCHC	5 cut(s)	294, 590, 731, 1422, 1503
SfaNI	GCATC	4 cut(s)	324, 1359, 1657, 1740
Sfr274I	CTCGAG	1 cut(s)	1415
SinI	GGWCC	6 cut(s)	67, 421, 649, 709, 924, 1628
SlaI	CTCGAG	1 cut(s)	1415
SmiMI	CAYNNNNRTG	3 cut(s)	1221, 1280, 1547
SmlI	CTYRAG	3 cut(s)	62, 1415, 1502
SmoI	CTYRAG	3 cut(s)	62, 1415, 1502
Sse9I	AATT	9 cut(s)	208, 240, 462, 698, 845, 953, 1021, 1177, 1618
SseBI	AGGCCT	1 cut(s)	493
SsiI	CCGC	7 cut(s)	100, 529, 678, 788, 1031, 1043, 1457
SspMI	CTAG	1 cut(s)	1196
SstI	GAGCTC	2 cut(s)	294, 1503
StuI	AGGCCT	1 cut(s)	493
StyD4I	CCNGG	8 cut(s)	567, 687, 758, 1474, 1581, 1692, 1724, 1766
StyI	CCWWGG	3 cut(s)	8, 424, 927
TaaI	ACNGT	5 cut(s)	41, 346, 1136, 1189, 1570
TaiI	ACGT	1 cut(s)	260
TaqI	TCGA	6 cut(s)	234, 781, 1086, 1353, 1416, 1440
TaqII	GACCGA	1 cut(s)	152
TasI	AATT	9 cut(s)	208, 240, 462, 698, 845, 953, 1021, 1177, 1618
TatI	WGTACW	7 cut(s)	91, 467, 609, 634, 900, 1443, 1713
TauI	GCSGC	1 cut(s)	1045
TfiI	GAWTC	6 cut(s)	16, 814, 1320, 1756, 1763, 1782
Tru1I	TTAA	3 cut(s)	552, 876, 1181
Tru9I	TTAA	3 cut(s)	552, 876, 1181
TscAI	CASTG	6 cut(s)	46, 292, 719, 760, 1575, 1638
TseFI	GTSAC	6 cut(s)	916, 1214, 1234, 1271, 1285, 1393
TseI	GCWGC	3 cut(s)	97, 1560, 1651
Tsp45I	GTSAC	6 cut(s)	916, 1214, 1234, 1271, 1285, 1393
TspDTI	ATGAA	6 cut(s)	102, 228, 413, 1239, 1312, 1386
TspGWI	ACGGA	1 cut(s)	1373
TspRI	CASTG	6 cut(s)	46, 292, 719, 760, 1575, 1638
VpaK11BI	GGWCC	6 cut(s)	67, 421, 649, 709, 924, 1628
XapI	RAATTY	4 cut(s)	698, 845, 953, 1177
XcmI	CCANNNNNNNNNTGG	3 cut(s)	623, 1630, 1756
XhoI	CTCGAG	1 cut(s)	1415
XmnI	GAANNNNTTC	3 cut(s)	182, 368, 1380
XspI	CTAG	1 cut(s)	1196
ZraI	GACGTC	1 cut(s)	258
ZrmI	AGTACT	1 cut(s)	469
Zsp2I	ATGCAT	1 cut(s)	85

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.