MD16G1148900.v1.1

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0021867
Physical Location & Seq
Forward (+)
11706761 .. 11709070
2310 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1148900.v1.1.491

Sequence Viewer

Length: 918 bp
ATGTCGCAGACCCATTTCAGCAAGGGCGACGAGGTCGAGGTCGTCACGAAGCCCTCCCGCAGTTCCACCGGGCCGTACTACCCCGCGACGGTGCGCCGCCCCGTGGTCAAGGACAAGGCCCACATTTTAGTCCAGTACCAAACCCTGACGACCCCCAACGCCGTCAGCCCCAGACTCCTCCAGGAGATAGTCGAATTAGGCAATGTGAGGCCCGCACCGCCGAGGGAGCTCTACCAGTTCTTCAAGGTGGGCGACGACGTCGACGCCTACAGAGAAAATGGGTGGTCTCGCGGGACTGTGACGGACATTCTGGAGAACTCCAAGTACTTAGTTGCGCTGCGGGGTGAGGAATTTGAGTGCGAGCAGTGTGATATGAGGATTCACAGAGACTGGGATGATGGCTCTTGGGTCCCTCCTATTCAAGAAGAGCTTTGTAATAGTTCATCTGGGATGGTGGTGAAATCTAGAAAATTAATATTGAAATTTAAGTGCGGCAAGAAACCACTGGGAGCAAGCTTTGAAAATGGGACTGTGGTGGAGGTCAGCAGCGACGAGGAAGGCTACAAGGATGCTTGGTACACTGCTAAAATAATTGATCATGTAGGAAATAACAAGTATCTGGTGGAATACCTGTACCTGGTAACGGATGACGGAACAAAGGATCCCTTGAGAGAAGAAACATGTGCAAGTTATATTAGACCTTGTCCTCCTGCACTTCCATCGGTTGCTCATTTCAAAGTGCTCCAGAAAGTCGATGCTTGGTACAATGAGGGATGGTGGGAGGGCACCGTTTCCAAAGTTCTTGGCGGCTTAATGTATGTTGTCTATTTCAGCTCCACCAATGAAGAATTAATGTTTGAACATTCCAATTTGAGGCCTCGACAGGACTGGATTAACGGACAATGGATCCCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

306

Amino Acids

34.78

Weight (kDa)

5.4

Isoelectric Point (pI)

37.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 9 - 74 3.4e-07 Agenet domain
Agenet PF05641 176 - 237 4.9e-18 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021867)

Species Orthologous Gene IDs
malus_domestica MD16G1148900.v1.1
prunus_persica Prupe.1G112600_v2.0.a1 Prupe.1G112600_v2.0.a1
pyrus_communis pycom16g12710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 261
AccB1I GGYRCC 1 cut(s) 785
AccI GTMKAC 1 cut(s) 261
AccII CGCG 2 cut(s) 86, 291
AciI CCGC 9 cut(s) 58, 84, 97, 213, 218, 291, 340, 492, 807
AclWI GGATC 4 cut(s) 656, 669, 901, 914
AcsI RAATTY 2 cut(s) 350, 482
AcyI GRCGYC 2 cut(s) 258, 264
AfaI GTAC 6 cut(s) 77, 137, 326, 578, 635, 764
AfiI CCNNNNNNNGG 5 cut(s) 88, 103, 637, 643, 873
AflIII ACRYGT 1 cut(s) 680
AgsI TTSAA 6 cut(s) 244, 422, 481, 521, 736, 860
AjnI CCWGG 2 cut(s) 180, 636
AloI GAACNNNNNNTCC 2 cut(s) 646, 678
AluBI AGCT 4 cut(s) 229, 430, 516, 834
AluI AGCT 4 cut(s) 229, 430, 516, 834
Alw21I GWGCWC 2 cut(s) 231, 744
Alw26I GTCTC 2 cut(s) 291, 381
AlwI GGATC 4 cut(s) 656, 669, 901, 914
AlwNI CAGNNNCTG 1 cut(s) 390
AoxI GGCC 4 cut(s) 71, 117, 209, 875
ApeKI GCWGC 2 cut(s) 337, 546
ApoI RAATTY 2 cut(s) 350, 482
AseI ATTAAT 2 cut(s) 473, 851
AspLEI GCGC 2 cut(s) 96, 337
AspS9I GGNCC 4 cut(s) 71, 118, 210, 409
AsuC2I CCSGG 1 cut(s) 70
AsuHPI GGTGA 2 cut(s) 356, 469
AvaII GGWCC 1 cut(s) 409
BaeGI GKGCMC 1 cut(s) 788
BamHI GGATCC 2 cut(s) 661, 906
BanI GGYRCC 1 cut(s) 785
BanII GRGCYC 1 cut(s) 231
Bbv12I GWGCWC 2 cut(s) 231, 744
BbvI GCAGC 2 cut(s) 324, 558
BccI CCATC 4 cut(s) 392, 445, 727, 768
BceAI ACGGC 2 cut(s) 58, 146
BcgI CGANNNNNNTGC 2 cut(s) 702, 736
BciT130I CCWGG 2 cut(s) 182, 638
BclI TGATCA 1 cut(s) 595
BcnI CCSGG 1 cut(s) 70
BcoDI GTCTC 2 cut(s) 291, 381
BfaI CTAG 1 cut(s) 465
BfmI CTRYAG 1 cut(s) 268
BisI GCNGC 5 cut(s) 97, 338, 493, 547, 808
BlsI GCNGC 5 cut(s) 98, 339, 494, 548, 809
BmcAI AGTACT 1 cut(s) 326
Bme1390I CCNGG 3 cut(s) 70, 182, 638
Bme18I GGWCC 1 cut(s) 409
BmgT120I GGNCC 4 cut(s) 71, 118, 210, 409
BmiI GGNNCC 5 cut(s) 410, 411, 663, 787, 908
BmrFI CCNGG 3 cut(s) 70, 182, 638
BmrI ACTGGG 2 cut(s) 400, 515
BmsI GCATC 2 cut(s) 559, 745
BmuI ACTGGG 2 cut(s) 400, 515
BpmI CTGGAG 3 cut(s) 164, 332, 728
BpuEI CTTGAG 1 cut(s) 688
BpuMI CCSGG 1 cut(s) 70
BsaHI GRCGYC 2 cut(s) 258, 264
BsaI GGTCTC 1 cut(s) 291
BsaJI CCNNGG 2 cut(s) 102, 221
Bsc4I CCNNNNNNNGG 5 cut(s) 88, 103, 637, 643, 873
Bse1I ACTGG 5 cut(s) 133, 235, 395, 510, 893
Bse3DI GCAATG 1 cut(s) 208
BseBI CCWGG 2 cut(s) 182, 638
BseDI CCNNGG 2 cut(s) 102, 221
BseGI GGATG 5 cut(s) 400, 456, 574, 652, 779
BseLI CCNNNNNNNGG 5 cut(s) 88, 103, 637, 643, 873
BseMI GCAATG 1 cut(s) 208
BseNI ACTGG 5 cut(s) 133, 235, 395, 510, 893
BseRI GAGGAG 1 cut(s) 167
BseSI GKGCMC 1 cut(s) 788
BseXI GCAGC 2 cut(s) 324, 558
BsgI GTGCAG 1 cut(s) 696
Bsh1236I CGCG 2 cut(s) 86, 291
BshFI GGCC 4 cut(s) 73, 119, 211, 877
BshNI GGYRCC 1 cut(s) 785
BsiHKAI GWGCWC 2 cut(s) 231, 744
BsiSI CCGG 1 cut(s) 69
BslFI GGGAC 3 cut(s) 307, 395, 541
BslI CCNNNNNNNGG 5 cut(s) 88, 103, 637, 643, 873
BsmAI GTCTC 2 cut(s) 291, 381
BsmFI GGGAC 3 cut(s) 307, 395, 541
BsnI GGCC 4 cut(s) 73, 119, 211, 877
Bso31I GGTCTC 1 cut(s) 291
Bsp1286I GDGCHC 3 cut(s) 231, 744, 788
Bsp143I GATC 3 cut(s) 595, 661, 906
BspACI CCGC 9 cut(s) 58, 84, 97, 213, 218, 291, 340, 492, 807
BspANI GGCC 4 cut(s) 73, 119, 211, 877
BspFNI CGCG 2 cut(s) 86, 291
BspLI GGNNCC 5 cut(s) 410, 411, 663, 787, 908
BspPI GGATC 4 cut(s) 656, 669, 901, 914
BspQI GCTCTTC 1 cut(s) 420
BspT107I GGYRCC 1 cut(s) 785
BspTNI GGTCTC 1 cut(s) 291
BsrDI GCAATG 1 cut(s) 208
BsrI ACTGG 5 cut(s) 133, 235, 395, 510, 893
BssECI CCNNGG 2 cut(s) 102, 221
BssMI GATC 3 cut(s) 595, 661, 906
BssNI GRCGYC 2 cut(s) 258, 264
Bst2UI CCWGG 2 cut(s) 182, 638
Bst4CI ACNGT 4 cut(s) 91, 298, 532, 790
Bst6I CTCTTC 1 cut(s) 420
BstACI GRCGYC 2 cut(s) 258, 264
BstC8I GCNNGC 3 cut(s) 213, 362, 514
BstDEI CTNAG 1 cut(s) 328
BstDSI CCRYGG 1 cut(s) 102
BstF5I GGATG 5 cut(s) 400, 456, 574, 652, 779
BstFNI CGCG 2 cut(s) 86, 291
BstHHI GCGC 2 cut(s) 96, 337
BstKTI GATC 3 cut(s) 598, 664, 909
BstMAI GTCTC 2 cut(s) 291, 381
BstMBI GATC 3 cut(s) 595, 661, 906
BstMWI GCNNNNNNNGC 2 cut(s) 217, 226
BstNI CCWGG 2 cut(s) 182, 638
BstNSI RCATGY 1 cut(s) 684
BstSCI CCNGG 3 cut(s) 68, 180, 636
BstSFI CTRYAG 1 cut(s) 268
BstSLI GKGCMC 1 cut(s) 788
BstUI CGCG 2 cut(s) 86, 291
BstV1I GCAGC 2 cut(s) 324, 558
BstX2I RGATCY 2 cut(s) 661, 906
BstYI RGATCY 2 cut(s) 661, 906
BsuRI GGCC 4 cut(s) 73, 119, 211, 877
BtgI CCRYGG 1 cut(s) 102
BtsCI GGATG 5 cut(s) 400, 456, 574, 652, 779
BtsI GCAGTG 2 cut(s) 371, 579
BtsIMutI CAGTG 3 cut(s) 371, 503, 579
Cac8I GCNNGC 3 cut(s) 213, 362, 514
CaiI CAGNNNCTG 1 cut(s) 390
CfoI GCGC 2 cut(s) 96, 337
Cfr13I GGNCC 4 cut(s) 71, 118, 210, 409
CseI GACGC 1 cut(s) 272
CsiI ACCWGGT 1 cut(s) 636
Csp6I GTAC 6 cut(s) 76, 136, 325, 577, 634, 763
CviAII CATG 2 cut(s) 599, 681
CviQI GTAC 6 cut(s) 76, 136, 325, 577, 634, 763
DdeI CTNAG 1 cut(s) 328
DpnI GATC 3 cut(s) 597, 663, 908
DpnII GATC 3 cut(s) 595, 661, 906
Eam1104I CTCTTC 1 cut(s) 420
EarI CTCTTC 1 cut(s) 420
Ecl136II GAGCTC 1 cut(s) 229
Eco147I AGGCCT 1 cut(s) 877
Eco24I GRGCYC 1 cut(s) 231
Eco31I GGTCTC 1 cut(s) 291
Eco47I GGWCC 1 cut(s) 409
Eco53kI GAGCTC 1 cut(s) 229
EcoICRI GAGCTC 1 cut(s) 229
EcoO109I RGGNCCY 1 cut(s) 409
EcoRII CCWGG 2 cut(s) 180, 636
EcoT38I GRGCYC 1 cut(s) 231
FaeI CATG 2 cut(s) 602, 684
FaiI YATR 5 cut(s) 374, 600, 682, 693, 819
FalI AAGNNNNNCTT 4 cut(s) 414, 446, 650, 682
FaqI GGGAC 3 cut(s) 307, 395, 541
FatI CATG 2 cut(s) 598, 680
FauI CCCGC 5 cut(s) 65, 91, 220, 284, 333
FbaI TGATCA 1 cut(s) 595
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 5 cut(s) 97, 338, 493, 547, 808
FokI GGATG 5 cut(s) 407, 463, 581, 659, 786
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 5 cut(s) 97, 338, 493, 547, 808
FspBI CTAG 1 cut(s) 465
GlaI GCGC 2 cut(s) 95, 336
GluI GCNGC 5 cut(s) 97, 338, 493, 547, 808
GsuI CTGGAG 3 cut(s) 164, 332, 728
HaeIII GGCC 4 cut(s) 73, 119, 211, 877
HapII CCGG 1 cut(s) 69
HgaI GACGC 1 cut(s) 272
HhaI GCGC 2 cut(s) 96, 337
Hin1I GRCGYC 2 cut(s) 258, 264
Hin1II CATG 2 cut(s) 602, 684
Hin6I GCGC 2 cut(s) 94, 335
HinP1I GCGC 2 cut(s) 94, 335
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HindIII AAGCTT 1 cut(s) 514
HinfI GANTC 2 cut(s) 174, 379
HpaII CCGG 1 cut(s) 69
HphI GGTGA 2 cut(s) 356, 469
Hpy166II GTNNAC 2 cut(s) 262, 579
Hpy188III TCNNGA 5 cut(s) 46, 311, 422, 465, 745
Hpy8I GTNNAC 2 cut(s) 262, 579
Hpy99I CGWCG 7 cut(s) 32, 91, 257, 260, 263, 266, 554
HpyAV CCTTC 1 cut(s) 551
HpyCH4III ACNGT 4 cut(s) 91, 298, 532, 790
HpyCH4IV ACGT 1 cut(s) 258
HpyCH4V TGCA 2 cut(s) 686, 713
HpyF10VI GCNNNNNNNGC 2 cut(s) 217, 226
HpyF3I CTNAG 1 cut(s) 328
HpySE526I ACGT 1 cut(s) 258
Hsp92I GRCGYC 2 cut(s) 258, 264
Hsp92II CATG 2 cut(s) 602, 684
HspAI GCGC 2 cut(s) 94, 335
KflI GGGWCCC 1 cut(s) 409
Ksp22I TGATCA 1 cut(s) 595
Kzo9I GATC 3 cut(s) 595, 661, 906
LguI GCTCTTC 1 cut(s) 420
LmnI GCTCC 4 cut(s) 226, 509, 747, 839
Lsp1109I GCAGC 2 cut(s) 324, 558
LweI GCATC 2 cut(s) 559, 745
MabI ACCWGGT 1 cut(s) 636
MaeI CTAG 1 cut(s) 465
MaeII ACGT 1 cut(s) 258
MaeIII GTNAC 3 cut(s) 43, 298, 640
MalI GATC 3 cut(s) 597, 663, 908
MboI GATC 3 cut(s) 595, 661, 906
MboII GAAGA 4 cut(s) 232, 437, 686, 857
MflI RGATCY 2 cut(s) 661, 906
MhlI GDGCHC 3 cut(s) 231, 744, 788
MluCI AATT 7 cut(s) 194, 350, 470, 482, 591, 848, 868
MlyI GAGTC 1 cut(s) 168
MseI TTAA 6 cut(s) 473, 486, 812, 851, 894, 916
MspI CCGG 1 cut(s) 69
MspR9I CCNGG 3 cut(s) 70, 182, 638
MvaI CCWGG 2 cut(s) 182, 638
MvnI CGCG 2 cut(s) 86, 291
MwoI GCNNNNNNNGC 2 cut(s) 217, 226
NciI CCSGG 1 cut(s) 70
NdeII GATC 3 cut(s) 595, 661, 906
NlaIII CATG 2 cut(s) 602, 684
NlaIV GGNNCC 5 cut(s) 410, 411, 663, 787, 908
NmeAIII GCCGAG 1 cut(s) 246
NmuCI GTSAC 2 cut(s) 43, 298
NspI RCATGY 1 cut(s) 684
PceI AGGCCT 1 cut(s) 877
PciI ACATGT 1 cut(s) 680
PciSI GCTCTTC 1 cut(s) 420
PfeI GAWTC 1 cut(s) 379
PflFI GACNNNGTC 3 cut(s) 32, 257, 702
PfoI TCCNGGA 1 cut(s) 180
PkrI GCNGC 5 cut(s) 98, 339, 494, 548, 809
PleI GAGTC 1 cut(s) 168
PpsI GAGTC 1 cut(s) 168
PpuMI RGGWCCY 1 cut(s) 409
PscI ACATGT 1 cut(s) 680
PshBI ATTAAT 2 cut(s) 473, 851
Psp124BI GAGCTC 1 cut(s) 231
Psp5II RGGWCCY 1 cut(s) 409
Psp6I CCWGG 2 cut(s) 180, 636
PspGI CCWGG 2 cut(s) 180, 636
PspN4I GGNNCC 5 cut(s) 410, 411, 663, 787, 908
PspPI GGNCC 4 cut(s) 71, 118, 210, 409
PspPPI RGGWCCY 1 cut(s) 409
PsrI GAACNNNNNNTAC 2 cut(s) 308, 340
PstNI CAGNNNCTG 1 cut(s) 390
PsuI RGATCY 2 cut(s) 661, 906
PsyI GACNNNGTC 3 cut(s) 32, 257, 702
RsaI GTAC 6 cut(s) 77, 137, 326, 578, 635, 764
RsaNI GTAC 6 cut(s) 76, 136, 325, 577, 634, 763
SacI GAGCTC 1 cut(s) 231
SalI GTCGAC 1 cut(s) 260
SapI GCTCTTC 1 cut(s) 420
SaqAI TTAA 6 cut(s) 473, 486, 812, 851, 894, 916
SatI GCNGC 5 cut(s) 97, 338, 493, 547, 808
Sau3AI GATC 3 cut(s) 595, 661, 906
Sau96I GGNCC 4 cut(s) 71, 118, 210, 409
ScaI AGTACT 1 cut(s) 326
SchI GAGTC 1 cut(s) 168
ScrFI CCNGG 3 cut(s) 70, 182, 638
SduI GDGCHC 3 cut(s) 231, 744, 788
SexAI ACCWGGT 1 cut(s) 636
SfaNI GCATC 2 cut(s) 559, 745
SfcI CTRYAG 1 cut(s) 268
SgrDI CGTCGACG 1 cut(s) 260
SinI GGWCC 1 cut(s) 409
SmlI CTYRAG 1 cut(s) 667
SmoI CTYRAG 1 cut(s) 667
Sse9I AATT 7 cut(s) 194, 350, 470, 482, 591, 848, 868
SseBI AGGCCT 1 cut(s) 877
SsiI CCGC 9 cut(s) 58, 84, 97, 213, 218, 291, 340, 492, 807
SspI AATATT 1 cut(s) 477
SspMI CTAG 1 cut(s) 465
SstI GAGCTC 1 cut(s) 231
StuI AGGCCT 1 cut(s) 877
StyD4I CCNGG 3 cut(s) 68, 180, 636
TaaI ACNGT 4 cut(s) 91, 298, 532, 790
TaiI ACGT 1 cut(s) 261
TaqI TCGA 5 cut(s) 36, 192, 261, 753, 880
TasI AATT 7 cut(s) 194, 350, 470, 482, 591, 848, 868
TatI WGTACW 1 cut(s) 324
TauI GCSGC 3 cut(s) 99, 495, 810
TfiI GAWTC 1 cut(s) 379
Tru1I TTAA 6 cut(s) 473, 486, 812, 851, 894, 916
Tru9I TTAA 6 cut(s) 473, 486, 812, 851, 894, 916
TscAI CASTG 3 cut(s) 371, 510, 586
TseFI GTSAC 2 cut(s) 43, 298
TseI GCWGC 2 cut(s) 337, 546
Tsp45I GTSAC 2 cut(s) 43, 298
TspDTI ATGAA 2 cut(s) 432, 858
TspGWI ACGGA 4 cut(s) 317, 659, 666, 912
TspRI CASTG 3 cut(s) 371, 510, 586
Tth111I GACNNNGTC 3 cut(s) 32, 257, 702
VpaK11BI GGWCC 1 cut(s) 409
VspI ATTAAT 2 cut(s) 473, 851
XapI RAATTY 2 cut(s) 350, 482
XbaI TCTAGA 1 cut(s) 464
XceI RCATGY 1 cut(s) 684
XmiI GTMKAC 1 cut(s) 261
XspI CTAG 1 cut(s) 465
ZraI GACGTC 1 cut(s) 259
ZrmI AGTACT 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.