pycom16g12710

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0021867
Physical Location & Seq
Forward (+)
8987657 .. 8989103
1447 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g12710.1

Sequence Viewer

Length: 690 bp
ATGATCGCCACTCCACCACTCTACCGCTCCGTCACTCCACCGCTCTACTCCCTTCTCCCGGCTGCTAGGGTTTCCGATTCCCAAATGTCGCAGACCCATTTCAGCAAGGGCGACGAAGTCGAGGTCGTCACGAAGCCCTCCCGCGGTTCCACCGGGCCGTACTACCCCGCGACGGTGCGCCGCTCCGTGGTCAAGGACAAGGCCCACATTTTAGTCCAGTACCAAACCCTGACGACCCCCAACGCCGTCAGCCCCAGACTCCTCCAGGAGATAGTCGAATTAGGCAATGTGAGGCCCGCACCGCCGAGGGAGCTCTACCAGTTCTTCAAGGTGGGCGACGACGTCGACGCCTACAGAGAAAATGGGTGGTCTCGCGGGACTGTGACGGACATTCTGGAGAACTCCAAGTACTTAGTTGCGCTGCGGGGTGAGGAATTTGAGTGCGAGCAGTGTGATTTGAGGATTCACAGAGACTGGGATGATGGCTCTTGGGTCCCTCCTTTTCAAGAAGAGAATAGTTCGTCTGGGATGGTGGTGAAATCTAGAAAGTTAGTATTGAAATTTAAGTGCGGCAAGAAACCATTGGGAGCAAGCTTTGAAAACGGGACTGTGGTGGAGGTCAGCAGCGACGAGGAAGGCTACAAGGATGCTTGGTACACTGCGAAAATAATTGATCGATGTAGGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

230

Amino Acids

25.84

Weight (kDa)

6.84

Isoelectric Point (pI)

40.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 37 - 102 1.4e-08 Agenet domain
Agenet PF05641 202 - 226 1.2e-06 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021867)

Species Orthologous Gene IDs
malus_domestica MD16G1148900.v1.1
prunus_persica Prupe.1G112600_v2.0.a1 Prupe.1G112600_v2.0.a1
pyrus_communis pycom16g12710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 345
AccBSI CCGCTC 3 cut(s) 27, 43, 183
AccI GTMKAC 1 cut(s) 345
AccII CGCG 3 cut(s) 144, 170, 375
AcsI RAATTY 2 cut(s) 434, 560
AcyI GRCGYC 2 cut(s) 342, 348
AfaI GTAC 4 cut(s) 161, 221, 410, 656
AfiI CCNNNNNNNGG 4 cut(s) 58, 143, 172, 187
AgsI TTSAA 4 cut(s) 328, 506, 559, 599
AjnI CCWGG 1 cut(s) 264
AluBI AGCT 2 cut(s) 313, 594
AluI AGCT 2 cut(s) 313, 594
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 2 cut(s) 375, 465
AlwNI CAGNNNCTG 1 cut(s) 474
AoxI GGCC 3 cut(s) 155, 201, 293
ApeKI GCWGC 3 cut(s) 62, 421, 624
ApoI RAATTY 2 cut(s) 434, 560
ArsI GACNNNNNNTTYG 2 cut(s) 108, 140
AspLEI GCGC 2 cut(s) 180, 421
AspS9I GGNCC 4 cut(s) 155, 202, 294, 493
AsuC2I CCSGG 2 cut(s) 59, 154
AsuHPI GGTGA 2 cut(s) 440, 547
AvaII GGWCC 1 cut(s) 493
BanII GRGCYC 1 cut(s) 315
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 3 cut(s) 49, 408, 636
BccI CCATC 2 cut(s) 476, 523
BceAI ACGGC 2 cut(s) 142, 230
BciT130I CCWGG 1 cut(s) 266
BcnI CCSGG 2 cut(s) 59, 154
BcoDI GTCTC 2 cut(s) 375, 465
BfaI CTAG 2 cut(s) 66, 543
BfmI CTRYAG 1 cut(s) 352
BisI GCNGC 5 cut(s) 63, 181, 422, 571, 625
BlsI GCNGC 5 cut(s) 64, 182, 423, 572, 626
BmcAI AGTACT 1 cut(s) 410
Bme1390I CCNGG 3 cut(s) 59, 154, 266
Bme18I GGWCC 1 cut(s) 493
BmgT120I GGNCC 4 cut(s) 155, 202, 294, 493
BmiI GGNNCC 3 cut(s) 148, 494, 495
BmrFI CCNGG 3 cut(s) 59, 154, 266
BmrI ACTGGG 1 cut(s) 484
BmsI GCATC 1 cut(s) 637
BmuI ACTGGG 1 cut(s) 484
BpmI CTGGAG 2 cut(s) 248, 416
BpuMI CCSGG 2 cut(s) 59, 154
Bsa29I ATCGAT 1 cut(s) 676
BsaHI GRCGYC 2 cut(s) 342, 348
BsaI GGTCTC 1 cut(s) 375
BsaJI CCNNGG 3 cut(s) 142, 186, 305
Bsc4I CCNNNNNNNGG 4 cut(s) 58, 143, 172, 187
Bse1I ACTGG 3 cut(s) 217, 319, 479
Bse3DI GCAATG 1 cut(s) 292
BseBI CCWGG 1 cut(s) 266
BseCI ATCGAT 1 cut(s) 676
BseDI CCNNGG 3 cut(s) 142, 186, 305
BseGI GGATG 3 cut(s) 484, 534, 652
BseLI CCNNNNNNNGG 4 cut(s) 58, 143, 172, 187
BseMI GCAATG 1 cut(s) 292
BseNI ACTGG 3 cut(s) 217, 319, 479
BseRI GAGGAG 1 cut(s) 251
BseXI GCAGC 3 cut(s) 49, 408, 636
Bsh1236I CGCG 3 cut(s) 144, 170, 375
BshFI GGCC 3 cut(s) 157, 203, 295
BshVI ATCGAT 1 cut(s) 676
BsiHKAI GWGCWC 1 cut(s) 315
BsiSI CCGG 2 cut(s) 59, 153
BslFI GGGAC 3 cut(s) 391, 479, 619
BslI CCNNNNNNNGG 4 cut(s) 58, 143, 172, 187
BsmAI GTCTC 2 cut(s) 375, 465
BsmFI GGGAC 3 cut(s) 391, 479, 619
BsnI GGCC 3 cut(s) 157, 203, 295
Bso31I GGTCTC 1 cut(s) 375
Bsp1286I GDGCHC 1 cut(s) 315
Bsp143I GATC 2 cut(s) 3, 673
BspANI GGCC 3 cut(s) 157, 203, 295
BspDI ATCGAT 1 cut(s) 676
BspFNI CGCG 3 cut(s) 144, 170, 375
BspLI GGNNCC 3 cut(s) 148, 494, 495
BspTNI GGTCTC 1 cut(s) 375
BsrBI CCGCTC 3 cut(s) 27, 43, 183
BsrDI GCAATG 1 cut(s) 292
BsrI ACTGG 3 cut(s) 217, 319, 479
BssECI CCNNGG 3 cut(s) 142, 186, 305
BssMI GATC 2 cut(s) 3, 673
BssNI GRCGYC 2 cut(s) 342, 348
Bst2UI CCWGG 1 cut(s) 266
Bst4CI ACNGT 3 cut(s) 175, 382, 610
Bst6I CTCTTC 1 cut(s) 504
BstACI GRCGYC 2 cut(s) 342, 348
BstC8I GCNNGC 3 cut(s) 297, 446, 592
BstDEI CTNAG 1 cut(s) 412
BstDSI CCRYGG 2 cut(s) 142, 186
BstF5I GGATG 3 cut(s) 484, 534, 652
BstFNI CGCG 3 cut(s) 144, 170, 375
BstHHI GCGC 2 cut(s) 180, 421
BstKTI GATC 2 cut(s) 6, 676
BstMAI GTCTC 2 cut(s) 375, 465
BstMBI GATC 2 cut(s) 3, 673
BstMWI GCNNNNNNNGC 2 cut(s) 301, 310
BstNI CCWGG 1 cut(s) 266
BstSCI CCNGG 3 cut(s) 57, 152, 264
BstSFI CTRYAG 1 cut(s) 352
BstUI CGCG 3 cut(s) 144, 170, 375
BstV1I GCAGC 3 cut(s) 49, 408, 636
Bsu15I ATCGAT 1 cut(s) 676
BsuRI GGCC 3 cut(s) 157, 203, 295
BsuTUI ATCGAT 1 cut(s) 676
BtgI CCRYGG 2 cut(s) 142, 186
BtsCI GGATG 3 cut(s) 484, 534, 652
BtsI GCAGTG 2 cut(s) 455, 657
BtsIMutI CAGTG 2 cut(s) 455, 657
Cac8I GCNNGC 3 cut(s) 297, 446, 592
CaiI CAGNNNCTG 1 cut(s) 474
CfoI GCGC 2 cut(s) 180, 421
Cfr13I GGNCC 4 cut(s) 155, 202, 294, 493
Cfr42I CCGCGG 1 cut(s) 145
ClaI ATCGAT 1 cut(s) 676
CseI GACGC 1 cut(s) 356
Csp6I GTAC 4 cut(s) 160, 220, 409, 655
CviQI GTAC 4 cut(s) 160, 220, 409, 655
DdeI CTNAG 1 cut(s) 412
DpnI GATC 2 cut(s) 5, 675
DpnII GATC 2 cut(s) 3, 673
Eam1104I CTCTTC 1 cut(s) 504
EarI CTCTTC 1 cut(s) 504
Ecl136II GAGCTC 1 cut(s) 313
Eco24I GRGCYC 1 cut(s) 315
Eco31I GGTCTC 1 cut(s) 375
Eco47I GGWCC 1 cut(s) 493
Eco53kI GAGCTC 1 cut(s) 313
EcoICRI GAGCTC 1 cut(s) 313
EcoO109I RGGNCCY 1 cut(s) 493
EcoRII CCWGG 1 cut(s) 264
EcoT38I GRGCYC 1 cut(s) 315
FaqI GGGAC 3 cut(s) 391, 479, 619
FauI CCCGC 5 cut(s) 149, 175, 304, 368, 417
FblI GTMKAC 1 cut(s) 345
Fnu4HI GCNGC 5 cut(s) 63, 181, 422, 571, 625
FokI GGATG 3 cut(s) 491, 541, 659
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 5 cut(s) 63, 181, 422, 571, 625
FspBI CTAG 2 cut(s) 66, 543
GlaI GCGC 2 cut(s) 179, 420
GluI GCNGC 5 cut(s) 63, 181, 422, 571, 625
GsuI CTGGAG 2 cut(s) 248, 416
HaeIII GGCC 3 cut(s) 157, 203, 295
HapII CCGG 2 cut(s) 59, 153
HgaI GACGC 1 cut(s) 356
HhaI GCGC 2 cut(s) 180, 421
Hin1I GRCGYC 2 cut(s) 342, 348
Hin6I GCGC 2 cut(s) 178, 419
HinP1I GCGC 2 cut(s) 178, 419
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HindIII AAGCTT 1 cut(s) 592
HinfI GANTC 3 cut(s) 77, 258, 463
HpaII CCGG 2 cut(s) 59, 153
HphI GGTGA 2 cut(s) 440, 547
Hpy166II GTNNAC 2 cut(s) 346, 657
Hpy188I TCNGA 1 cut(s) 76
Hpy188III TCNNGA 4 cut(s) 130, 395, 506, 543
Hpy8I GTNNAC 2 cut(s) 346, 657
Hpy99I CGWCG 7 cut(s) 116, 175, 341, 344, 347, 350, 632
HpyAV CCTTC 2 cut(s) 62, 629
HpyCH4III ACNGT 3 cut(s) 175, 382, 610
HpyCH4IV ACGT 1 cut(s) 342
HpyF10VI GCNNNNNNNGC 2 cut(s) 301, 310
HpyF3I CTNAG 1 cut(s) 412
HpySE526I ACGT 1 cut(s) 342
Hsp92I GRCGYC 2 cut(s) 342, 348
HspAI GCGC 2 cut(s) 178, 419
KflI GGGWCCC 1 cut(s) 493
KspI CCGCGG 1 cut(s) 145
Kzo9I GATC 2 cut(s) 3, 673
LmnI GCTCC 4 cut(s) 32, 188, 310, 587
Lsp1109I GCAGC 3 cut(s) 49, 408, 636
LweI GCATC 1 cut(s) 637
MaeI CTAG 2 cut(s) 66, 543
MaeII ACGT 1 cut(s) 342
MaeIII GTNAC 3 cut(s) 31, 127, 382
MalI GATC 2 cut(s) 5, 675
MbiI CCGCTC 3 cut(s) 27, 43, 183
MboI GATC 2 cut(s) 3, 673
MboII GAAGA 2 cut(s) 316, 521
MhlI GDGCHC 1 cut(s) 315
MluCI AATT 4 cut(s) 278, 434, 560, 669
MlyI GAGTC 1 cut(s) 252
MseI TTAA 1 cut(s) 564
MspA1I CMGCKG 1 cut(s) 144
MspI CCGG 2 cut(s) 59, 153
MspR9I CCNGG 3 cut(s) 59, 154, 266
MvaI CCWGG 1 cut(s) 266
MvnI CGCG 3 cut(s) 144, 170, 375
MwoI GCNNNNNNNGC 2 cut(s) 301, 310
NciI CCSGG 2 cut(s) 59, 154
NdeII GATC 2 cut(s) 3, 673
NlaIV GGNNCC 3 cut(s) 148, 494, 495
NmeAIII GCCGAG 1 cut(s) 330
NmuCI GTSAC 3 cut(s) 31, 127, 382
PfeI GAWTC 2 cut(s) 77, 463
PflFI GACNNNGTC 2 cut(s) 116, 341
PfoI TCCNGGA 1 cut(s) 264
PkrI GCNGC 5 cut(s) 64, 182, 423, 572, 626
PleI GAGTC 1 cut(s) 252
PpsI GAGTC 1 cut(s) 252
PpuMI RGGWCCY 1 cut(s) 493
Psp124BI GAGCTC 1 cut(s) 315
Psp5II RGGWCCY 1 cut(s) 493
Psp6I CCWGG 1 cut(s) 264
PspGI CCWGG 1 cut(s) 264
PspN4I GGNNCC 3 cut(s) 148, 494, 495
PspPI GGNCC 4 cut(s) 155, 202, 294, 493
PspPPI RGGWCCY 1 cut(s) 493
PsrI GAACNNNNNNTAC 2 cut(s) 392, 424
PstNI CAGNNNCTG 1 cut(s) 474
PsyI GACNNNGTC 2 cut(s) 116, 341
RsaI GTAC 4 cut(s) 161, 221, 410, 656
RsaNI GTAC 4 cut(s) 160, 220, 409, 655
SacI GAGCTC 1 cut(s) 315
SacII CCGCGG 1 cut(s) 145
SalI GTCGAC 1 cut(s) 344
SaqAI TTAA 1 cut(s) 564
SatI GCNGC 5 cut(s) 63, 181, 422, 571, 625
Sau3AI GATC 2 cut(s) 3, 673
Sau96I GGNCC 4 cut(s) 155, 202, 294, 493
ScaI AGTACT 1 cut(s) 410
SchI GAGTC 1 cut(s) 252
ScrFI CCNGG 3 cut(s) 59, 154, 266
SduI GDGCHC 1 cut(s) 315
SetI ASST 6 cut(s) 126, 315, 333, 345, 596, 621
SfaNI GCATC 1 cut(s) 637
SfcI CTRYAG 1 cut(s) 352
Sfr303I CCGCGG 1 cut(s) 145
SgrBI CCGCGG 1 cut(s) 145
SgrDI CGTCGACG 1 cut(s) 344
SinI GGWCC 1 cut(s) 493
Sse9I AATT 4 cut(s) 278, 434, 560, 669
SspMI CTAG 2 cut(s) 66, 543
SstI GAGCTC 1 cut(s) 315
StyD4I CCNGG 3 cut(s) 57, 152, 264
TaaI ACNGT 3 cut(s) 175, 382, 610
TaiI ACGT 1 cut(s) 345
TaqI TCGA 4 cut(s) 120, 276, 345, 676
TasI AATT 4 cut(s) 278, 434, 560, 669
TatI WGTACW 1 cut(s) 408
TauI GCSGC 2 cut(s) 183, 573
TfiI GAWTC 2 cut(s) 77, 463
Tru1I TTAA 1 cut(s) 564
Tru9I TTAA 1 cut(s) 564
TscAI CASTG 2 cut(s) 455, 664
TseFI GTSAC 3 cut(s) 31, 127, 382
TseI GCWGC 3 cut(s) 62, 421, 624
Tsp45I GTSAC 3 cut(s) 31, 127, 382
TspGWI ACGGA 3 cut(s) 19, 175, 401
TspRI CASTG 2 cut(s) 455, 664
Tth111I GACNNNGTC 2 cut(s) 116, 341
VpaK11BI GGWCC 1 cut(s) 493
XapI RAATTY 2 cut(s) 434, 560
XbaI TCTAGA 1 cut(s) 542
XmiI GTMKAC 1 cut(s) 345
XspI CTAG 2 cut(s) 66, 543
ZraI GACGTC 1 cut(s) 343
ZrmI AGTACT 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.