MD16G1155900.v1.1

Ribose-phosphate pyrophosphokinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0024352
Physical Location & Seq
Reverse (-)
12415282 .. 12416292
1011 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1155900.v1.1.491

Sequence Viewer

Length: 1011 bp
ATGATTGTGAGACGTCTTTATGATTTGGTGCCAATTTCTTCAGCCACTGAAGCATATTTGATGAATGAAATTGGCAAAAATGATGTCAGGCACAAGTATTTTTCCTGTCCTACAAACCCAGTGCTATCTCAGGAAATTGCTTACTACATGGGTCTGGAACTTGGATGGATTATGATCAAAGGCTTTGCTGATGAAGAGATATGTGTTCAGTTGCATGAGAGTGTAAGAGGGTGTGATGTATATCTTGTGCAACCCACATGTCCTCCTACAAATGAAAAATTTATGGAGTTTCTTGTCATGAGTGGTGCTTGCCGGCTGAATGTGGGTAGGAGGATCCATGAGTATATGTTGAAGATGAGATTAACGGAGAATTCACAGGTTACGAATGCACAGCTGGATCTTTATGCAAAGAGTGAAGACAATATCATTACTGGGATATCTTATAATGCCATAAAGATAGCAATAAAGCATTGGATCAATTTTGGTACACCACCACGTACTACTGTCAAGAGCTATTTTCCTTCAACCAATCTTGCGGTGTGTTTATCAGTGCCATTTTCCCATGCCTGGGTACACTTGAAGACCTTGCTTTATGCTTTGTTGAGCCTAAACTTGTCAAGGTCCTGCAACATTAATAATGTCATGACTATTCTAGGCTTTGCTTATACTTACACCTTATTAAATTCCCTCATTTTGGGAACATCTTTCTATTCTACAATTGAGACATCCTCAGTGCTTTCAAGTTGTGTGAAACAACCAAAATTTATTTTGTGCGATGGTAGGTATCCTTCAGCCTTCACTCCTACATACTCAACATTAATTCTTAGGACTGAACCTCACTCCAAGAGAGTTCAGGTTACATGGGAAGTTCAGTTGGATGTCTTTCATGGAGCTATCATAGGTGCGAATCATGTGCACGGGTTCAGACCATCCATGTCCAGTGGGTTGTATTCGTGCAACTTCACCAGCATGCTTTTCCCCACCCCACAATTTTCTATCTGGAACAGCTGA

Protein Analysis

337

Amino Acids

38.01

Weight (kDa)

8.46

Isoelectric Point (pI)

51.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pribosyltran_N PF13793 32 - 112 3.7e-20 N-terminal domain of ribose phosphate pyrophosphokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024352)

Species Orthologous Gene IDs
malus_domestica MD16G1155900.v1.1
pyrus_communis pycom03g13570 pycom15g23730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 444
AatII GACGTC 1 cut(s) 16
AccB1I GGYRCC 1 cut(s) 28
AciI CCGC 1 cut(s) 536
AclWI GGATC 4 cut(s) 328, 341, 405, 482
AcsI RAATTY 4 cut(s) 278, 370, 682, 761
AcuI CTGAAG 3 cut(s) 24, 69, 774
AcyI GRCGYC 1 cut(s) 13
AfaI GTAC 3 cut(s) 487, 499, 573
AfiI CCNNNNNNNGG 3 cut(s) 567, 568, 694
AflIII ACRYGT 1 cut(s) 257
AgsI TTSAA 4 cut(s) 352, 525, 580, 741
AjnI CCWGG 1 cut(s) 566
AluBI AGCT 4 cut(s) 394, 513, 893, 1008
AluI AGCT 4 cut(s) 394, 513, 893, 1008
Alw21I GWGCWC 1 cut(s) 918
Alw26I GTCTC 2 cut(s) 4, 716
Alw44I GTGCAC 1 cut(s) 914
AlwI GGATC 4 cut(s) 328, 341, 405, 482
AlwNI CAGNNNCTG 1 cut(s) 47
ApaLI GTGCAC 1 cut(s) 914
ApoI RAATTY 4 cut(s) 278, 370, 682, 761
ArsI GACNNNNNNTTYG 2 cut(s) 69, 101
AseI ATTAAT 2 cut(s) 633, 818
AspS9I GGNCC 1 cut(s) 621
AsuHPI GGTGA 1 cut(s) 955
AvaII GGWCC 1 cut(s) 621
BaeGI GKGCMC 1 cut(s) 918
BamHI GGATCC 1 cut(s) 333
BanI GGYRCC 1 cut(s) 28
BarI GAAGNNNNNNTAC 2 cut(s) 772, 804
BbsI GAAGAC 2 cut(s) 423, 587
Bbv12I GWGCWC 1 cut(s) 918
BccI CCATC 3 cut(s) 159, 770, 937
BcgI CGANNNNNNTGC 2 cut(s) 895, 929
BciT130I CCWGG 1 cut(s) 568
BciVI GTATCC 1 cut(s) 795
BclI TGATCA 1 cut(s) 174
BcoDI GTCTC 2 cut(s) 4, 716
BfaI CTAG 1 cut(s) 653
BfuI GTATCC 1 cut(s) 795
Bme1390I CCNGG 1 cut(s) 568
Bme18I GGWCC 1 cut(s) 621
BmgT120I GGNCC 1 cut(s) 621
BmiI GGNNCC 2 cut(s) 30, 335
BmrFI CCNGG 1 cut(s) 568
BmrI ACTGGG 2 cut(s) 113, 441
BmuI ACTGGG 2 cut(s) 113, 441
BpiI GAAGAC 2 cut(s) 423, 587
BplI GAGNNNNNCTC 2 cut(s) 713, 745
BsaAI YACGTR 1 cut(s) 497
BsaBI GATNNNNATC 2 cut(s) 173, 240
BsaHI GRCGYC 1 cut(s) 13
BsaJI CCNNGG 1 cut(s) 567
BsaXI ACNNNNNCTCC 2 cut(s) 247, 277
Bsc4I CCNNNNNNNGG 3 cut(s) 567, 568, 694
Bse118I RCCGGY 1 cut(s) 312
Bse1I ACTGG 3 cut(s) 119, 436, 939
Bse8I GATNNNNATC 2 cut(s) 173, 240
BseBI CCWGG 1 cut(s) 568
BseDI CCNNGG 1 cut(s) 567
BseGI GGATG 4 cut(s) 170, 725, 883, 929
BseJI GATNNNNATC 2 cut(s) 173, 240
BseLI CCNNNNNNNGG 3 cut(s) 567, 568, 694
BseMII CTCAG 2 cut(s) 143, 744
BseNI ACTGG 3 cut(s) 119, 436, 939
BseSI GKGCMC 1 cut(s) 918
BshNI GGYRCC 1 cut(s) 28
BsiHKAI GWGCWC 1 cut(s) 918
BsiSI CCGG 1 cut(s) 313
BslI CCNNNNNNNGG 3 cut(s) 567, 568, 694
BsmAI GTCTC 2 cut(s) 4, 716
BsmBI CGTCTC 1 cut(s) 4
BsmI GAATGC 1 cut(s) 391
Bsp1286I GDGCHC 1 cut(s) 918
Bsp143I GATC 4 cut(s) 174, 333, 397, 474
BspACI CCGC 1 cut(s) 536
BspCNI CTCAG 2 cut(s) 142, 743
BspHI TCATGA 2 cut(s) 297, 642
BspLI GGNNCC 2 cut(s) 30, 335
BspPI GGATC 4 cut(s) 328, 341, 405, 482
BspT107I GGYRCC 1 cut(s) 28
BsrFI RCCGGY 1 cut(s) 312
BsrI ACTGG 3 cut(s) 119, 436, 939
BssAI RCCGGY 1 cut(s) 312
BssECI CCNNGG 1 cut(s) 567
BssMI GATC 4 cut(s) 174, 333, 397, 474
BssNI GRCGYC 1 cut(s) 13
Bst2UI CCWGG 1 cut(s) 568
Bst4CI ACNGT 1 cut(s) 505
Bst6I CTCTTC 1 cut(s) 189
BstACI GRCGYC 1 cut(s) 13
BstBAI YACGTR 1 cut(s) 497
BstC8I GCNNGC 3 cut(s) 310, 314, 971
BstDEI CTNAG 3 cut(s) 129, 730, 824
BstF5I GGATG 4 cut(s) 170, 725, 883, 929
BstKTI GATC 4 cut(s) 177, 336, 400, 477
BstMAI GTCTC 2 cut(s) 4, 716
BstMBI GATC 4 cut(s) 174, 333, 397, 474
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstNI CCWGG 1 cut(s) 568
BstNSI RCATGY 2 cut(s) 261, 973
BstSCI CCNGG 1 cut(s) 566
BstSLI GKGCMC 1 cut(s) 918
BstV2I GAAGAC 2 cut(s) 423, 587
BstX2I RGATCY 2 cut(s) 333, 397
BstYI RGATCY 2 cut(s) 333, 397
BsuI GTATCC 1 cut(s) 795
BtgZI GCGATG 1 cut(s) 789
BtsCI GGATG 4 cut(s) 170, 725, 883, 929
BtsIMutI CAGTG 5 cut(s) 45, 126, 555, 738, 946
Cac8I GCNNGC 3 cut(s) 310, 314, 971
CaiI CAGNNNCTG 1 cut(s) 47
CciI TCATGA 2 cut(s) 297, 642
Cfr10I RCCGGY 1 cut(s) 312
Cfr13I GGNCC 1 cut(s) 621
Csp6I GTAC 3 cut(s) 486, 498, 572
CviQI GTAC 3 cut(s) 486, 498, 572
DdeI CTNAG 3 cut(s) 129, 730, 824
DpnI GATC 4 cut(s) 176, 335, 399, 476
DpnII GATC 4 cut(s) 174, 333, 397, 474
Eam1104I CTCTTC 1 cut(s) 189
EarI CTCTTC 1 cut(s) 189
Eco32I GATATC 1 cut(s) 438
Eco47I GGWCC 1 cut(s) 621
Eco57I CTGAAG 3 cut(s) 24, 69, 774
EcoO109I RGGNCCY 1 cut(s) 621
EcoRI GAATTC 1 cut(s) 370
EcoRII CCWGG 1 cut(s) 566
EcoRV GATATC 1 cut(s) 438
Esp3I CGTCTC 1 cut(s) 4
FbaI TGATCA 1 cut(s) 174
FokI GGATG 4 cut(s) 177, 712, 890, 916
FspBI CTAG 1 cut(s) 653
HapII CCGG 1 cut(s) 313
Hin1I GRCGYC 1 cut(s) 13
HinfI GANTC 1 cut(s) 907
HpaII CCGG 1 cut(s) 313
HphI GGTGA 1 cut(s) 955
Hpy166II GTNNAC 3 cut(s) 488, 574, 916
Hpy188I TCNGA 1 cut(s) 926
Hpy188III TCNNGA 6 cut(s) 131, 155, 298, 508, 643, 1000
Hpy8I GTNNAC 3 cut(s) 488, 574, 916
HpyAV CCTTC 3 cut(s) 531, 798, 805
HpyCH4III ACNGT 1 cut(s) 505
HpyCH4IV ACGT 2 cut(s) 13, 496
HpyCH4V TGCA 7 cut(s) 214, 250, 389, 407, 627, 916, 957
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
HpyF3I CTNAG 3 cut(s) 129, 730, 824
HpySE526I ACGT 2 cut(s) 13, 496
Hsp92I GRCGYC 1 cut(s) 13
KroI GCCGGC 1 cut(s) 312
KroNI GCCGGC 1 cut(s) 314
Ksp22I TGATCA 1 cut(s) 174
Kzo9I GATC 4 cut(s) 174, 333, 397, 474
LmnI GCTCC 1 cut(s) 890
MaeI CTAG 1 cut(s) 653
MaeII ACGT 2 cut(s) 13, 496
MaeIII GTNAC 2 cut(s) 379, 856
MalI GATC 4 cut(s) 176, 335, 399, 476
MboI GATC 4 cut(s) 174, 333, 397, 474
MboII GAAGA 5 cut(s) 30, 206, 364, 428, 592
MfeI CAATTG 1 cut(s) 717
MflI RGATCY 2 cut(s) 333, 397
MhlI GDGCHC 1 cut(s) 918
MmeI TCCRAC 1 cut(s) 855
MnlI CCTC 6 cut(s) 221, 273, 324, 698, 739, 846
MroNI GCCGGC 1 cut(s) 312
MseI TTAA 4 cut(s) 362, 633, 680, 818
MslI CAYNNNNRTG 2 cut(s) 219, 968
MspA1I CMGCKG 2 cut(s) 394, 1008
MspI CCGG 1 cut(s) 313
MspR9I CCNGG 1 cut(s) 568
MunI CAATTG 1 cut(s) 717
Mva1269I GAATGC 1 cut(s) 391
MvaI CCWGG 1 cut(s) 568
MwoI GCNNNNNNNGC 1 cut(s) 50
NaeI GCCGGC 1 cut(s) 314
NdeII GATC 4 cut(s) 174, 333, 397, 474
NgoMIV GCCGGC 1 cut(s) 312
NlaIV GGNNCC 2 cut(s) 30, 335
NspI RCATGY 2 cut(s) 261, 973
PaeI GCATGC 1 cut(s) 973
PagI TCATGA 2 cut(s) 297, 642
PciI ACATGT 1 cut(s) 257
PctI GAATGC 1 cut(s) 391
PdiI GCCGGC 1 cut(s) 314
PfeI GAWTC 1 cut(s) 907
Ppu21I YACGTR 1 cut(s) 497
PpuMI RGGWCCY 1 cut(s) 621
PscI ACATGT 1 cut(s) 257
PshBI ATTAAT 2 cut(s) 633, 818
PsiI TTATAA 1 cut(s) 444
Psp5II RGGWCCY 1 cut(s) 621
Psp6I CCWGG 1 cut(s) 566
PspGI CCWGG 1 cut(s) 566
PspN4I GGNNCC 2 cut(s) 30, 335
PspPI GGNCC 1 cut(s) 621
PspPPI RGGWCCY 1 cut(s) 621
PstNI CAGNNNCTG 1 cut(s) 47
PsuI RGATCY 2 cut(s) 333, 397
PvuII CAGCTG 2 cut(s) 394, 1008
RsaI GTAC 3 cut(s) 487, 499, 573
RsaNI GTAC 3 cut(s) 486, 498, 572
RseI CAYNNNNRTG 2 cut(s) 219, 968
SaqAI TTAA 4 cut(s) 362, 633, 680, 818
Sau3AI GATC 4 cut(s) 174, 333, 397, 474
Sau96I GGNCC 1 cut(s) 621
ScrFI CCNGG 1 cut(s) 568
SduI GDGCHC 1 cut(s) 918
SinI GGWCC 1 cut(s) 621
SmiMI CAYNNNNRTG 2 cut(s) 219, 968
SphI GCATGC 1 cut(s) 973
SsiI CCGC 1 cut(s) 536
SspMI CTAG 1 cut(s) 653
StyD4I CCNGG 1 cut(s) 566
TaaI ACNGT 1 cut(s) 505
TaiI ACGT 2 cut(s) 16, 499
TfiI GAWTC 1 cut(s) 907
Tru1I TTAA 4 cut(s) 362, 633, 680, 818
Tru9I TTAA 4 cut(s) 362, 633, 680, 818
TscAI CASTG 5 cut(s) 52, 126, 555, 738, 946
TspDTI ATGAA 5 cut(s) 77, 81, 207, 288, 875
TspGWI ACGGA 1 cut(s) 380
TspRI CASTG 5 cut(s) 52, 126, 555, 738, 946
VneI GTGCAC 1 cut(s) 914
VpaK11BI GGWCC 1 cut(s) 621
VspI ATTAAT 2 cut(s) 633, 818
XapI RAATTY 4 cut(s) 278, 370, 682, 761
XceI RCATGY 2 cut(s) 261, 973
XspI CTAG 1 cut(s) 653
ZraI GACGTC 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.