pycom03g13570

Phosphoribosyl synthetase-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0024352
Physical Location & Seq
Reverse (-)
14679405 .. 14680076
672 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g13570.1

Sequence Viewer

Length: 672 bp
ATGGGTCTTGAATTTGGATGGATTATGATAAAAGGCTTTGCTGATGAAGAGATATATGTTCAGTTGCATGAGAGTGTAAGAGGGTCTGGTGTATATCTTGTGCAACTCACATGTCCTCCTGCAAATGAAAATTTTATTCTTGTCATGAGTGGTGCTTGTCGGCTGACTGTGGGTAGGAGGATCCATGAGTATATGTTGAAGGTGGGATTAACGAGGAATTCACAGGTTACGAATGCACAGCTGGATCTTTATGCAAAGAGTGAAAACAAAATCATTATTGGGATATCTTATAATGCCATAAAGATAGCAATAAAGCCTTGGATCAATTTGGTACACCACCATGTACTGCTGTCGAGCTATTTTTCCTTGACTAATCTTGAGGTGTGTTTGTCAGTGCCATTTTCCCATGCCTGGGTATACTTGAAGACCATATATACTTTGTTGAACCTAAACTTGTCAAGGTCTTGCAACATTAATAATGTCATGACTATTCCAGACTTTGCTTATACTTACAGCTTATTAAATTTCCTCATTTTGGGAACATCTTTCTATTCTACAATTGAGACCTCCTCAGTGCTTTCAAGTTGTGCGAGGCAACCAAAATTTACTGTGCACTGGTGGGTATCCTTCAGCCTTCACTCCTACATACTCAACATTACTTATTGGGACTGA

Protein Analysis

224

Amino Acids

25.49

Weight (kDa)

8.24

Isoelectric Point (pI)

38.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pribosyltran_N PF13793 2 - 62 6.6e-10 N-terminal domain of ribose phosphate pyrophosphokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0024352)

Species Orthologous Gene IDs
malus_domestica MD16G1155900.v1.1
pyrus_communis pycom03g13570 pycom15g23730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 291
AccI GTMKAC 1 cut(s) 417
AclWI GGATC 4 cut(s) 175, 188, 252, 329
AcsI RAATTY 5 cut(s) 11, 130, 217, 523, 602
AcuI CTGAAG 1 cut(s) 613
AfaI GTAC 2 cut(s) 333, 345
AfiI CCNNNNNNNGG 3 cut(s) 411, 412, 535
AflIII ACRYGT 1 cut(s) 110
AgsI TTSAA 5 cut(s) 11, 199, 424, 445, 582
AjnI CCWGG 1 cut(s) 410
AluBI AGCT 3 cut(s) 241, 357, 516
AluI AGCT 3 cut(s) 241, 357, 516
Alw21I GWGCWC 1 cut(s) 615
Alw26I GTCTC 1 cut(s) 557
Alw44I GTGCAC 1 cut(s) 611
AlwI GGATC 4 cut(s) 175, 188, 252, 329
ApaLI GTGCAC 1 cut(s) 611
ApoI RAATTY 5 cut(s) 11, 130, 217, 523, 602
AseI ATTAAT 1 cut(s) 474
BaeGI GKGCMC 1 cut(s) 615
BamHI GGATCC 1 cut(s) 180
BbsI GAAGAC 1 cut(s) 431
Bbv12I GWGCWC 1 cut(s) 615
BccI CCATC 1 cut(s) 12
BciT130I CCWGG 1 cut(s) 412
BciVI GTATCC 1 cut(s) 634
BcoDI GTCTC 1 cut(s) 557
BfuI GTATCC 1 cut(s) 634
Bme1390I CCNGG 1 cut(s) 412
BmiI GGNNCC 1 cut(s) 182
BmrFI CCNGG 1 cut(s) 412
BpiI GAAGAC 1 cut(s) 431
BplI GAGNNNNNCTC 2 cut(s) 554, 586
BpuEI CTTGAG 1 cut(s) 398
BsaI GGTCTC 1 cut(s) 557
BsaJI CCNNGG 2 cut(s) 317, 411
BsaXI ACNNNNNCTCC 2 cut(s) 100, 130
Bsc4I CCNNNNNNNGG 3 cut(s) 411, 412, 535
Bse1I ACTGG 1 cut(s) 620
BseBI CCWGG 1 cut(s) 412
BseDI CCNNGG 2 cut(s) 317, 411
BseGI GGATG 1 cut(s) 23
BseLI CCNNNNNNNGG 3 cut(s) 411, 412, 535
BseMII CTCAG 1 cut(s) 585
BseNI ACTGG 1 cut(s) 620
BseRI GAGGAG 1 cut(s) 559
BseSI GKGCMC 1 cut(s) 615
BsiHKAI GWGCWC 1 cut(s) 615
BslI CCNNNNNNNGG 3 cut(s) 411, 412, 535
BsmAI GTCTC 1 cut(s) 557
BsmI GAATGC 1 cut(s) 238
Bso31I GGTCTC 1 cut(s) 557
Bsp1286I GDGCHC 1 cut(s) 615
Bsp143I GATC 3 cut(s) 180, 244, 321
BspCNI CTCAG 1 cut(s) 584
BspHI TCATGA 2 cut(s) 144, 483
BspLI GGNNCC 1 cut(s) 182
BspPI GGATC 4 cut(s) 175, 188, 252, 329
BspTNI GGTCTC 1 cut(s) 557
BsrI ACTGG 1 cut(s) 620
BssECI CCNNGG 2 cut(s) 317, 411
BssMI GATC 3 cut(s) 180, 244, 321
BssNAI GTATAC 1 cut(s) 418
BssT1I CCWWGG 1 cut(s) 317
Bst1107I GTATAC 1 cut(s) 418
Bst2UI CCWGG 1 cut(s) 412
Bst4CI ACNGT 2 cut(s) 169, 610
Bst6I CTCTTC 1 cut(s) 42
BstDEI CTNAG 1 cut(s) 571
BstF5I GGATG 1 cut(s) 23
BstKTI GATC 3 cut(s) 183, 247, 324
BstMAI GTCTC 1 cut(s) 557
BstMBI GATC 3 cut(s) 180, 244, 321
BstNI CCWGG 1 cut(s) 412
BstNSI RCATGY 1 cut(s) 114
BstSCI CCNGG 1 cut(s) 410
BstSLI GKGCMC 1 cut(s) 615
BstV2I GAAGAC 1 cut(s) 431
BstX2I RGATCY 2 cut(s) 180, 244
BstYI RGATCY 2 cut(s) 180, 244
BstZ17I GTATAC 1 cut(s) 418
BsuI GTATCC 1 cut(s) 634
BtsCI GGATG 1 cut(s) 23
BtsIMutI CAGTG 3 cut(s) 399, 579, 613
CciI TCATGA 2 cut(s) 144, 483
Csp6I GTAC 2 cut(s) 332, 344
CviAII CATG 7 cut(s) 68, 111, 145, 185, 341, 407, 484
CviJI RGCY 7 cut(s) 36, 163, 241, 316, 357, 516, 633
CviKI_1 RGCY 7 cut(s) 36, 163, 241, 316, 357, 516, 633
CviQI GTAC 2 cut(s) 332, 344
DdeI CTNAG 1 cut(s) 571
DpnI GATC 3 cut(s) 182, 246, 323
DpnII GATC 3 cut(s) 180, 244, 321
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
Eco130I CCWWGG 1 cut(s) 317
Eco31I GGTCTC 1 cut(s) 557
Eco32I GATATC 1 cut(s) 285
Eco57I CTGAAG 1 cut(s) 613
EcoRI GAATTC 1 cut(s) 217
EcoRII CCWGG 1 cut(s) 410
EcoRV GATATC 1 cut(s) 285
EcoT14I CCWWGG 1 cut(s) 317
ErhI CCWWGG 1 cut(s) 317
FaeI CATG 7 cut(s) 71, 114, 148, 188, 344, 410, 487
FatI CATG 7 cut(s) 67, 110, 144, 184, 340, 406, 483
FblI GTMKAC 1 cut(s) 417
FokI GGATG 1 cut(s) 30
Hin1II CATG 7 cut(s) 71, 114, 148, 188, 344, 410, 487
Hpy166II GTNNAC 3 cut(s) 334, 418, 613
Hpy188III TCNNGA 5 cut(s) 8, 145, 377, 484, 494
Hpy8I GTNNAC 3 cut(s) 334, 418, 613
HpyAV CCTTC 3 cut(s) 193, 637, 644
HpyCH4III ACNGT 2 cut(s) 169, 610
HpyCH4V TGCA 7 cut(s) 67, 103, 122, 236, 254, 468, 613
HpyF3I CTNAG 1 cut(s) 571
Hsp92II CATG 7 cut(s) 71, 114, 148, 188, 344, 410, 487
Kzo9I GATC 3 cut(s) 180, 244, 321
LpnPI CCDG 8 cut(s) 72, 132, 209, 227, 397, 424, 507, 601
MaeIII GTNAC 1 cut(s) 226
MalI GATC 3 cut(s) 182, 246, 323
MboI GATC 3 cut(s) 180, 244, 321
MboII GAAGA 2 cut(s) 59, 436
MfeI CAATTG 1 cut(s) 558
MflI RGATCY 2 cut(s) 180, 244
MhlI GDGCHC 1 cut(s) 615
MluCI AATT 7 cut(s) 11, 130, 217, 325, 523, 558, 602
MnlI CCTC 9 cut(s) 74, 126, 171, 207, 373, 539, 577, 580, 585
MseI TTAA 3 cut(s) 209, 474, 521
MslI CAYNNNNRTG 2 cut(s) 72, 339
MspA1I CMGCKG 1 cut(s) 241
MspR9I CCNGG 1 cut(s) 412
MunI CAATTG 1 cut(s) 558
Mva1269I GAATGC 1 cut(s) 238
MvaI CCWGG 1 cut(s) 412
NdeII GATC 3 cut(s) 180, 244, 321
NlaIII CATG 7 cut(s) 71, 114, 148, 188, 344, 410, 487
NlaIV GGNNCC 1 cut(s) 182
NspI RCATGY 1 cut(s) 114
PagI TCATGA 2 cut(s) 144, 483
PciI ACATGT 1 cut(s) 110
PctI GAATGC 1 cut(s) 238
PscI ACATGT 1 cut(s) 110
PshBI ATTAAT 1 cut(s) 474
PsiI TTATAA 1 cut(s) 291
Psp6I CCWGG 1 cut(s) 410
PspGI CCWGG 1 cut(s) 410
PspN4I GGNNCC 1 cut(s) 182
PsuI RGATCY 2 cut(s) 180, 244
PvuII CAGCTG 1 cut(s) 241
RsaI GTAC 2 cut(s) 333, 345
RsaNI GTAC 2 cut(s) 332, 344
RseI CAYNNNNRTG 2 cut(s) 72, 339
SaqAI TTAA 3 cut(s) 209, 474, 521
Sau3AI GATC 3 cut(s) 180, 244, 321
ScrFI CCNGG 1 cut(s) 412
SduI GDGCHC 1 cut(s) 615
SetI ASST 9 cut(s) 204, 228, 243, 359, 384, 450, 464, 518, 569
SmiMI CAYNNNNRTG 2 cut(s) 72, 339
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
Sse9I AATT 7 cut(s) 11, 130, 217, 325, 523, 558, 602
StyD4I CCNGG 1 cut(s) 410
StyI CCWWGG 1 cut(s) 317
TaaI ACNGT 2 cut(s) 169, 610
TaqI TCGA 1 cut(s) 353
TasI AATT 7 cut(s) 11, 130, 217, 325, 523, 558, 602
TatI WGTACW 1 cut(s) 343
Tru1I TTAA 3 cut(s) 209, 474, 521
Tru9I TTAA 3 cut(s) 209, 474, 521
TscAI CASTG 3 cut(s) 399, 579, 620
TspDTI ATGAA 2 cut(s) 60, 141
TspRI CASTG 3 cut(s) 399, 579, 620
VneI GTGCAC 1 cut(s) 611
VspI ATTAAT 1 cut(s) 474
XapI RAATTY 5 cut(s) 11, 130, 217, 523, 602
XceI RCATGY 1 cut(s) 114
XmiI GTMKAC 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.