MD16G1168200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0028549
Physical Location & Seq
Reverse (-)
13995113 .. 13995487
375 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1168200.v1.1.491

Sequence Viewer

Length: 375 bp
ATGATACATCAGGAGAGGCACGCCGGAACTGCCCCCCACGGGATTCTACTAGCGTTGGTGGTCGTGGCGTTGTTCGTGGTGCCGTCCTATGTAGGTGAACAAGGGGGGGCCATAATCGAAGCCTTCAGCGAGCTTTTGAGTCAGTTGGGTTTTCTCCTTCTCCCCATTATTCTCCTCGTGATCATCATTCAGTTCCTGTCTTCCAGCGGCTCCTTCGTCTCCTCGTTCTCGACCGGGGATCCCTACACCACCCACAGCATTAGTGGCTCGCCGTCGCCGGTTGGGGTTGCATTGTTTCTCTGCCTCGTTTTATTCCTTCTTTACAACAAGGTTTCGATCTTCGGCGGCGGCGGCGACGACGAAGGGGAGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

125

Amino Acids

13.01

Weight (kDa)

4.6

Isoelectric Point (pI)

48.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028549)

Species Orthologous Gene IDs
malus_domestica MD16G1168200.v1.1
pyrus_communis pycom16g14160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 79
AciI CCGC 4 cut(s) 207, 345, 348, 351
AclWI GGATC 2 cut(s) 233, 246
AcuI CTGAAG 1 cut(s) 109
AfiI CCNNNNNNNGG 1 cut(s) 39
AluBI AGCT 1 cut(s) 133
AluI AGCT 1 cut(s) 133
Alw26I GTCTC 1 cut(s) 223
AlwI GGATC 2 cut(s) 233, 246
AlwNI CAGNNNCTG 1 cut(s) 196
AoxI GGCC 1 cut(s) 108
AspS9I GGNCC 1 cut(s) 108
AsuC2I CCSGG 1 cut(s) 235
AsuHPI GGTGA 1 cut(s) 107
BamHI GGATCC 1 cut(s) 238
BanI GGYRCC 1 cut(s) 79
BauI CACGAG 1 cut(s) 176
BbsI GAAGAC 1 cut(s) 192
BceAI ACGGC 2 cut(s) 67, 256
BclI TGATCA 1 cut(s) 180
BcnI CCSGG 1 cut(s) 235
BcoDI GTCTC 1 cut(s) 223
BfaI CTAG 1 cut(s) 50
BisI GCNGC 4 cut(s) 208, 346, 349, 352
BlsI GCNGC 4 cut(s) 209, 347, 350, 353
Bme1390I CCNGG 1 cut(s) 235
BmgT120I GGNCC 1 cut(s) 108
BmiI GGNNCC 4 cut(s) 81, 109, 211, 240
BmrFI CCNGG 1 cut(s) 235
BpiI GAAGAC 1 cut(s) 192
BpuMI CCSGG 1 cut(s) 235
BsaJI CCNNGG 2 cut(s) 37, 234
Bsc4I CCNNNNNNNGG 1 cut(s) 39
Bse118I RCCGGY 1 cut(s) 277
BseDI CCNNGG 2 cut(s) 37, 234
BseLI CCNNNNNNNGG 1 cut(s) 39
BseRI GAGGAG 2 cut(s) 164, 211
Bsh1285I CGRYCG 1 cut(s) 234
BshFI GGCC 1 cut(s) 110
BshNI GGYRCC 1 cut(s) 79
BsiEI CGRYCG 1 cut(s) 234
BsiSI CCGG 3 cut(s) 24, 234, 278
BslI CCNNNNNNNGG 1 cut(s) 39
BsmAI GTCTC 1 cut(s) 223
BsmBI CGTCTC 1 cut(s) 223
BsnI GGCC 1 cut(s) 110
Bsp143I GATC 3 cut(s) 180, 238, 336
BspACI CCGC 4 cut(s) 207, 345, 348, 351
BspANI GGCC 1 cut(s) 110
BspLI GGNNCC 4 cut(s) 81, 109, 211, 240
BspPI GGATC 2 cut(s) 233, 246
BspT107I GGYRCC 1 cut(s) 79
BsrFI RCCGGY 1 cut(s) 277
BssAI RCCGGY 1 cut(s) 277
BssECI CCNNGG 2 cut(s) 37, 234
BssMI GATC 3 cut(s) 180, 238, 336
BssSI CACGAG 1 cut(s) 176
Bst2BI CACGAG 1 cut(s) 176
BstC8I GCNNGC 3 cut(s) 21, 131, 269
BstDSI CCRYGG 1 cut(s) 37
BstKTI GATC 3 cut(s) 183, 241, 339
BstMAI GTCTC 1 cut(s) 223
BstMBI GATC 3 cut(s) 180, 238, 336
BstMCI CGRYCG 1 cut(s) 234
BstMWI GCNNNNNNNGC 3 cut(s) 29, 264, 351
BstSCI CCNGG 1 cut(s) 233
BstV2I GAAGAC 1 cut(s) 192
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
BsuRI GGCC 1 cut(s) 110
BtgI CCRYGG 1 cut(s) 37
Cac8I GCNNGC 3 cut(s) 21, 131, 269
CaiI CAGNNNCTG 1 cut(s) 196
Cfr10I RCCGGY 1 cut(s) 277
Cfr13I GGNCC 1 cut(s) 108
CviJI RGCY 5 cut(s) 110, 122, 133, 210, 267
CviKI_1 RGCY 5 cut(s) 110, 122, 133, 210, 267
DpnI GATC 3 cut(s) 182, 240, 338
DpnII GATC 3 cut(s) 180, 238, 336
Eco57I CTGAAG 1 cut(s) 109
Esp3I CGTCTC 1 cut(s) 223
FaiI YATR 2 cut(s) 90, 113
FbaI TGATCA 1 cut(s) 180
Fnu4HI GCNGC 4 cut(s) 208, 346, 349, 352
Fsp4HI GCNGC 4 cut(s) 208, 346, 349, 352
FspBI CTAG 1 cut(s) 50
GluI GCNGC 4 cut(s) 208, 346, 349, 352
HaeIII GGCC 1 cut(s) 110
HapII CCGG 3 cut(s) 24, 234, 278
HinfI GANTC 2 cut(s) 43, 139
HpaII CCGG 3 cut(s) 24, 234, 278
HphI GGTGA 1 cut(s) 107
Hpy166II GTNNAC 1 cut(s) 98
Hpy188III TCNNGA 3 cut(s) 11, 178, 229
Hpy8I GTNNAC 1 cut(s) 98
Hpy99I CGWCG 3 cut(s) 277, 359, 362
HpyAV CCTTC 5 cut(s) 133, 167, 223, 326, 356
HpyCH4V TGCA 1 cut(s) 290
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 264, 351
Ksp22I TGATCA 1 cut(s) 180
Kzo9I GATC 3 cut(s) 180, 238, 336
LmnI GCTCC 1 cut(s) 215
LpnPI CCDG 5 cut(s) 37, 209, 217, 247, 291
MaeI CTAG 1 cut(s) 50
MalI GATC 3 cut(s) 182, 240, 338
MboI GATC 3 cut(s) 180, 238, 336
MboII GAAGA 2 cut(s) 192, 331
MflI RGATCY 1 cut(s) 238
MlyI GAGTC 1 cut(s) 148
MnlI CCTC 5 cut(s) 9, 185, 232, 314, 361
MspA1I CMGCKG 1 cut(s) 207
MspI CCGG 3 cut(s) 24, 234, 278
MspR9I CCNGG 1 cut(s) 235
MwoI GCNNNNNNNGC 3 cut(s) 29, 264, 351
NciI CCSGG 1 cut(s) 235
NdeII GATC 3 cut(s) 180, 238, 336
NlaIV GGNNCC 4 cut(s) 81, 109, 211, 240
PfeI GAWTC 1 cut(s) 43
PkrI GCNGC 4 cut(s) 209, 347, 350, 353
PleI GAGTC 1 cut(s) 147
PpsI GAGTC 1 cut(s) 147
PspN4I GGNNCC 4 cut(s) 81, 109, 211, 240
PspPI GGNCC 1 cut(s) 108
PstNI CAGNNNCTG 1 cut(s) 196
PsuI RGATCY 1 cut(s) 238
SatI GCNGC 4 cut(s) 208, 346, 349, 352
Sau3AI GATC 3 cut(s) 180, 238, 336
Sau96I GGNCC 1 cut(s) 108
SchI GAGTC 1 cut(s) 148
ScrFI CCNGG 1 cut(s) 235
SetI ASST 3 cut(s) 97, 135, 333
SsiI CCGC 4 cut(s) 207, 345, 348, 351
SspMI CTAG 1 cut(s) 50
StyD4I CCNGG 1 cut(s) 233
TaqI TCGA 3 cut(s) 117, 230, 335
TauI GCSGC 4 cut(s) 210, 348, 351, 354
TfiI GAWTC 1 cut(s) 43
XcmI CCANNNNNNNNNTGG 1 cut(s) 260
XspI CTAG 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.