MD16G1193600.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0021841
Physical Location & Seq
Reverse (-)
17187229 .. 17188194
966 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1193600.v1.1.491

Sequence Viewer

Length: 966 bp
ATGTTAGAATATGGGATCCCAATTAAGGCTGAATCGTACAATATGATCATCAAGATGCACGCCTTCCATAATCTTTGCCCCTCTGCTGTTCAGTATGCCAAGAAGTACTTTCTACAAATGCTTGAGAAGGGAATGCAGCCTGATTCCGGTACCTATACCCTCGTGATGGAGGCCATTGCCTGCCAGTCGGACAGCAAGACGAAGGTGATAGAGGAGGCCATAGAGTTTATGAAGCAAATTAAAGATAAGGGTCTTGTGCTTGACAACCCAAGTTATTTTGAGTTCCACGACCATCTTGAAGATGCAGTTGATACATTCAGGATGTTCGAATATGCCACCAGAATCGATGATGAGGAGATGAAAGAAATCTTCTTAAAGTGGCGCATCGATCGAGAGGCAGTCATGAAATTTGGATTTGATATGCTCAGTGGTCTGGTGGATGATAGCTATGGGGATGAGGCCTTTGAGCTTTTTGAGCCGGTCTTTGAGACGGGCATATATCCCTCATTTGTCATGCACACAACTATCATCCAGGCCTATGTTGCTGCTGGCAAGATTAAGGGAGCTTTCGATGCCTACCTTCACATGTTAGCGGCTAAGATCACTCATAACTTGTACACTTATAGTGTCCTCATCAAGGCACTCTCTGCTGATCCAAACTTGTTTAAGTCTGCCAAGGTTAACTTGCTGGATATGATGAAAAAGAAAATGCATCCTAACGCAGGTACCTACACAGCAGTGTTTGAGGGCTTTACTCGGCAGAGGGACAAGGGAGTGGAGAAGGGAAGAGAGTTTTTGAATCAGATGAAGGGCAATGGATTTGAGCCTGACGAGGGCGCTGTGAGGGAGGTGCTAAGAGGAAGAAAAGGTGAAGTGGTCCAAGAGGTCATGGAAATTCTGTTTGACAAATCTCACCTGCCTTGTGTTTCTTCCTTGACAAACAGGCTTCAGAACATCATACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

322

Amino Acids

36.79

Weight (kDa)

5.56

Isoelectric Point (pI)

31.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 169 - 215 9.1e-09 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021841)

Species Orthologous Gene IDs
malus_domestica MD16G1177800.v1.1 MD16G1193600.v1.1
pyrus_communis pycom16g14920 pycom16g16330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 924
Acc36I ACCTGC 2 cut(s) 713, 924
Acc65I GGTACC 2 cut(s) 149, 725
AccB1I GGYRCC 2 cut(s) 149, 725
AciI CCGC 1 cut(s) 593
AclWI GGATC 3 cut(s) 10, 23, 647
AcsI RAATTY 2 cut(s) 407, 894
AcuI CTGAAG 1 cut(s) 932
AfaI GTAC 5 cut(s) 38, 107, 151, 617, 727
AfiI CCNNNNNNNGG 6 cut(s) 25, 146, 166, 637, 722, 833
AflIII ACRYGT 1 cut(s) 585
AgsI TTSAA 2 cut(s) 299, 799
AjnI CCWGG 1 cut(s) 531
AleI CACNNNNGTG 1 cut(s) 737
AluBI AGCT 3 cut(s) 447, 469, 566
AluI AGCT 3 cut(s) 447, 469, 566
Alw26I GTCTC 1 cut(s) 482
AlwI GGATC 3 cut(s) 10, 23, 647
AoxI GGCC 4 cut(s) 171, 216, 459, 534
ApeKI GCWGC 2 cut(s) 136, 545
ApoI RAATTY 2 cut(s) 407, 894
Asp718I GGTACC 2 cut(s) 149, 725
AspLEI GCGC 2 cut(s) 384, 839
AspS9I GGNCC 1 cut(s) 877
AsuHPI GGTGA 3 cut(s) 217, 881, 905
AsuII TTCGAA 1 cut(s) 327
AvaII GGWCC 1 cut(s) 877
BaeI ACNNNNGTAYC 2 cut(s) 709, 742
BamHI GGATCC 1 cut(s) 15
BanI GGYRCC 2 cut(s) 149, 725
BauI CACGAG 1 cut(s) 161
BbvI GCAGC 2 cut(s) 148, 532
BccI CCATC 2 cut(s) 160, 300
BciT130I CCWGG 1 cut(s) 533
BclI TGATCA 1 cut(s) 45
BcoDI GTCTC 1 cut(s) 482
BfoI RGCGCY 1 cut(s) 840
BfuAI ACCTGC 2 cut(s) 713, 924
BisI GCNGC 3 cut(s) 137, 546, 594
BlsI GCNGC 3 cut(s) 138, 547, 595
BmcAI AGTACT 1 cut(s) 107
Bme1390I CCNGG 1 cut(s) 533
Bme18I GGWCC 1 cut(s) 877
BmgT120I GGNCC 1 cut(s) 877
BmiI GGNNCC 3 cut(s) 17, 151, 727
BmrFI CCNGG 1 cut(s) 533
BmsI GCATC 5 cut(s) 45, 292, 393, 562, 721
Bpu14I TTCGAA 1 cut(s) 327
BpuEI CTTGAG 1 cut(s) 143
Bsa29I ATCGAT 2 cut(s) 345, 387
BsaJI CCNNGG 1 cut(s) 675
BsaWI WCCGGW 1 cut(s) 146
Bsc4I CCNNNNNNNGG 6 cut(s) 25, 146, 166, 637, 722, 833
Bse118I RCCGGY 1 cut(s) 478
Bse1I ACTGG 1 cut(s) 184
Bse3DI GCAATG 2 cut(s) 174, 820
BseBI CCWGG 1 cut(s) 533
BseCI ATCGAT 2 cut(s) 345, 387
BseDI CCNNGG 1 cut(s) 675
BseGI GGATG 5 cut(s) 327, 445, 460, 528, 712
BseLI CCNNNNNNNGG 6 cut(s) 25, 146, 166, 637, 722, 833
BseMI GCAATG 2 cut(s) 174, 820
BseMII CTCAG 1 cut(s) 439
BseNI ACTGG 1 cut(s) 184
BseRI GAGGAG 2 cut(s) 227, 368
BseXI GCAGC 2 cut(s) 148, 532
Bsh1285I CGRYCG 1 cut(s) 391
BshFI GGCC 4 cut(s) 173, 218, 461, 536
BshNI GGYRCC 2 cut(s) 149, 725
BshVI ATCGAT 2 cut(s) 345, 387
BsiEI CGRYCG 1 cut(s) 391
BsiSI CCGG 2 cut(s) 147, 479
BslFI GGGAC 1 cut(s) 779
BslI CCNNNNNNNGG 6 cut(s) 25, 146, 166, 637, 722, 833
BsmAI GTCTC 1 cut(s) 482
BsmBI CGTCTC 1 cut(s) 482
BsmFI GGGAC 1 cut(s) 779
BsmI GAATGC 1 cut(s) 138
BsnI GGCC 4 cut(s) 173, 218, 461, 536
Bsp119I TTCGAA 1 cut(s) 327
Bsp1407I TGTACA 1 cut(s) 615
Bsp143I GATC 5 cut(s) 15, 45, 388, 600, 652
BspACI CCGC 1 cut(s) 593
BspANI GGCC 4 cut(s) 173, 218, 461, 536
BspCNI CTCAG 1 cut(s) 438
BspDI ATCGAT 2 cut(s) 345, 387
BspHI TCATGA 1 cut(s) 402
BspLI GGNNCC 3 cut(s) 17, 151, 727
BspMI ACCTGC 2 cut(s) 713, 924
BspPI GGATC 3 cut(s) 10, 23, 647
BspT104I TTCGAA 1 cut(s) 327
BspT107I GGYRCC 2 cut(s) 149, 725
BsrDI GCAATG 2 cut(s) 174, 820
BsrFI RCCGGY 1 cut(s) 478
BsrGI TGTACA 1 cut(s) 615
BsrI ACTGG 1 cut(s) 184
BssAI RCCGGY 1 cut(s) 478
BssECI CCNNGG 1 cut(s) 675
BssMI GATC 5 cut(s) 15, 45, 388, 600, 652
BssSI CACGAG 1 cut(s) 161
BssT1I CCWWGG 1 cut(s) 675
Bst2BI CACGAG 1 cut(s) 161
Bst2UI CCWGG 1 cut(s) 533
Bst4CI ACNGT 1 cut(s) 963
Bst6I CTCTTC 1 cut(s) 781
BstAPI GCANNNNNTGC 1 cut(s) 647
BstAUI TGTACA 1 cut(s) 615
BstBI TTCGAA 1 cut(s) 327
BstC8I GCNNGC 3 cut(s) 60, 181, 550
BstDEI CTNAG 3 cut(s) 425, 597, 854
BstENI CCTNNNNNAGG 2 cut(s) 635, 720
BstF5I GGATG 5 cut(s) 327, 445, 460, 528, 712
BstH2I RGCGCY 1 cut(s) 840
BstHHI GCGC 2 cut(s) 384, 839
BstKTI GATC 5 cut(s) 18, 48, 391, 603, 655
BstMAI GTCTC 1 cut(s) 482
BstMBI GATC 5 cut(s) 15, 45, 388, 600, 652
BstMCI CGRYCG 1 cut(s) 391
BstMWI GCNNNNNNNGC 4 cut(s) 475, 542, 572, 647
BstNI CCWGG 1 cut(s) 533
BstNSI RCATGY 1 cut(s) 589
BstSCI CCNGG 1 cut(s) 531
BstV1I GCAGC 2 cut(s) 148, 532
BstX2I RGATCY 1 cut(s) 15
BstYI RGATCY 1 cut(s) 15
Bsu15I ATCGAT 2 cut(s) 345, 387
BsuRI GGCC 4 cut(s) 173, 218, 461, 536
BsuTUI ATCGAT 2 cut(s) 345, 387
BtsCI GGATG 5 cut(s) 327, 445, 460, 528, 712
BtsI GCAGTG 1 cut(s) 744
BtsIMutI CAGTG 2 cut(s) 433, 744
BveI ACCTGC 2 cut(s) 713, 924
Cac8I GCNNGC 3 cut(s) 60, 181, 550
CciI TCATGA 1 cut(s) 402
CfoI GCGC 2 cut(s) 384, 839
Cfr10I RCCGGY 1 cut(s) 478
Cfr13I GGNCC 1 cut(s) 877
ClaI ATCGAT 2 cut(s) 345, 387
Csp6I GTAC 5 cut(s) 37, 106, 150, 616, 726
CviAII CATG 4 cut(s) 403, 514, 586, 889
CviQI GTAC 5 cut(s) 37, 106, 150, 616, 726
DdeI CTNAG 3 cut(s) 425, 597, 854
DpnI GATC 5 cut(s) 17, 47, 390, 602, 654
DpnII GATC 5 cut(s) 15, 45, 388, 600, 652
Eam1104I CTCTTC 1 cut(s) 781
EarI CTCTTC 1 cut(s) 781
Eco130I CCWWGG 1 cut(s) 675
Eco147I AGGCCT 2 cut(s) 461, 536
Eco47I GGWCC 1 cut(s) 877
Eco57I CTGAAG 1 cut(s) 932
EcoNI CCTNNNNNAGG 2 cut(s) 635, 720
EcoRII CCWGG 1 cut(s) 531
EcoT14I CCWWGG 1 cut(s) 675
EcoT22I ATGCAT 1 cut(s) 714
ErhI CCWWGG 1 cut(s) 675
Esp3I CGTCTC 1 cut(s) 482
FaeI CATG 4 cut(s) 406, 517, 589, 892
FalI AAGNNNNNCTT 4 cut(s) 92, 124, 668, 700
FaqI GGGAC 1 cut(s) 779
FatI CATG 4 cut(s) 402, 513, 585, 888
FbaI TGATCA 1 cut(s) 45
Fnu4HI GCNGC 3 cut(s) 137, 546, 594
FokI GGATG 5 cut(s) 334, 452, 467, 515, 699
Fsp4HI GCNGC 3 cut(s) 137, 546, 594
GlaI GCGC 2 cut(s) 383, 838
GluI GCNGC 3 cut(s) 137, 546, 594
HaeII RGCGCY 1 cut(s) 840
HaeIII GGCC 4 cut(s) 173, 218, 461, 536
HapII CCGG 2 cut(s) 147, 479
HhaI GCGC 2 cut(s) 384, 839
Hin1II CATG 4 cut(s) 406, 517, 589, 892
Hin6I GCGC 2 cut(s) 382, 837
HinP1I GCGC 2 cut(s) 382, 837
HincII GTYRAC 1 cut(s) 682
HindII GTYRAC 1 cut(s) 682
HinfI GANTC 4 cut(s) 32, 143, 342, 799
HpaI GTTAAC 1 cut(s) 682
HpaII CCGG 2 cut(s) 147, 479
HphI GGTGA 3 cut(s) 217, 881, 905
Hpy166II GTNNAC 2 cut(s) 618, 682
Hpy188I TCNGA 3 cut(s) 190, 804, 951
Hpy188III TCNNGA 6 cut(s) 52, 163, 296, 319, 392, 403
Hpy8I GTNNAC 2 cut(s) 618, 682
HpyAV CCTTC 6 cut(s) 73, 121, 196, 590, 775, 802
HpyCH4III ACNGT 1 cut(s) 963
HpyCH4V TGCA 5 cut(s) 58, 136, 305, 517, 712
HpyF10VI GCNNNNNNNGC 4 cut(s) 475, 542, 572, 647
HpyF3I CTNAG 3 cut(s) 425, 597, 854
Hsp92II CATG 4 cut(s) 406, 517, 589, 892
HspAI GCGC 2 cut(s) 382, 837
KpnI GGTACC 2 cut(s) 153, 729
Ksp22I TGATCA 1 cut(s) 45
KspAI GTTAAC 1 cut(s) 682
Kzo9I GATC 5 cut(s) 15, 45, 388, 600, 652
LmnI GCTCC 1 cut(s) 563
Lsp1109I GCAGC 2 cut(s) 148, 532
LweI GCATC 5 cut(s) 45, 292, 393, 562, 721
MalI GATC 5 cut(s) 17, 47, 390, 602, 654
MboI GATC 5 cut(s) 15, 45, 388, 600, 652
MboII GAAGA 5 cut(s) 311, 361, 798, 873, 921
MflI RGATCY 1 cut(s) 15
MluCI AATT 4 cut(s) 21, 237, 407, 894
MmeI TCCRAC 1 cut(s) 168
Mph1103I ATGCAT 1 cut(s) 714
MseI TTAA 6 cut(s) 24, 240, 374, 558, 666, 681
MslI CAYNNNNRTG 2 cut(s) 53, 737
MspI CCGG 2 cut(s) 147, 479
MspR9I CCNGG 1 cut(s) 533
Mva1269I GAATGC 1 cut(s) 138
MvaI CCWGG 1 cut(s) 533
MwoI GCNNNNNNNGC 4 cut(s) 475, 542, 572, 647
NdeII GATC 5 cut(s) 15, 45, 388, 600, 652
NlaIII CATG 4 cut(s) 406, 517, 589, 892
NlaIV GGNNCC 3 cut(s) 17, 151, 727
NmeAIII GCCGAG 1 cut(s) 736
NsiI ATGCAT 1 cut(s) 714
NspI RCATGY 1 cut(s) 589
NspV TTCGAA 1 cut(s) 327
OliI CACNNNNGTG 1 cut(s) 737
PagI TCATGA 1 cut(s) 402
PaqCI CACCTGC 1 cut(s) 924
PceI AGGCCT 2 cut(s) 461, 536
PciI ACATGT 1 cut(s) 585
PctI GAATGC 1 cut(s) 138
PfeI GAWTC 4 cut(s) 32, 143, 342, 799
PkrI GCNGC 3 cut(s) 138, 547, 595
Ple19I CGATCG 1 cut(s) 391
PscI ACATGT 1 cut(s) 585
Psp6I CCWGG 1 cut(s) 531
PspGI CCWGG 1 cut(s) 531
PspN4I GGNNCC 3 cut(s) 17, 151, 727
PspPI GGNCC 1 cut(s) 877
PsuI RGATCY 1 cut(s) 15
PvuI CGATCG 1 cut(s) 391
RsaI GTAC 5 cut(s) 38, 107, 151, 617, 727
RsaNI GTAC 5 cut(s) 37, 106, 150, 616, 726
RseI CAYNNNNRTG 2 cut(s) 53, 737
SaqAI TTAA 6 cut(s) 24, 240, 374, 558, 666, 681
SatI GCNGC 3 cut(s) 137, 546, 594
Sau3AI GATC 5 cut(s) 15, 45, 388, 600, 652
Sau96I GGNCC 1 cut(s) 877
ScaI AGTACT 1 cut(s) 107
ScrFI CCNGG 1 cut(s) 533
SfaNI GCATC 5 cut(s) 45, 292, 393, 562, 721
SfuI TTCGAA 1 cut(s) 327
SinI GGWCC 1 cut(s) 877
SmiMI CAYNNNNRTG 2 cut(s) 53, 737
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 4 cut(s) 21, 237, 407, 894
SseBI AGGCCT 2 cut(s) 461, 536
SsiI CCGC 1 cut(s) 593
StuI AGGCCT 2 cut(s) 461, 536
StyD4I CCNGG 1 cut(s) 531
StyI CCWWGG 1 cut(s) 675
TaaI ACNGT 1 cut(s) 963
TaqI TCGA 5 cut(s) 327, 345, 387, 391, 570
TasI AATT 4 cut(s) 21, 237, 407, 894
TatI WGTACW 2 cut(s) 105, 615
TauI GCSGC 1 cut(s) 596
TfiI GAWTC 4 cut(s) 32, 143, 342, 799
Tru1I TTAA 6 cut(s) 24, 240, 374, 558, 666, 681
Tru9I TTAA 6 cut(s) 24, 240, 374, 558, 666, 681
TscAI CASTG 2 cut(s) 433, 744
TseI GCWGC 2 cut(s) 136, 545
TspDTI ATGAA 5 cut(s) 245, 374, 419, 713, 821
TspRI CASTG 2 cut(s) 433, 744
VpaK11BI GGWCC 1 cut(s) 877
XagI CCTNNNNNAGG 2 cut(s) 635, 720
XapI RAATTY 2 cut(s) 407, 894
XceI RCATGY 1 cut(s) 589
ZrmI AGTACT 1 cut(s) 107
Zsp2I ATGCAT 1 cut(s) 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.