MD17G1004000.v1.1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0024436
Physical Location & Seq
Reverse (-)
345281 .. 346830
1550 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1004000.v1.1.491

Sequence Viewer

Length: 942 bp
ATGGAATCCAGCAGAGGAGGTGCGATCGTAGAAATCAAAGTGGTGTCGTGCAAAGATCTGAAAGCCTTCAATTTCTTCCAGAAGCTGTCAGTTTACGCGCTTGTGTCGGTGCAAAAGGATGACAATAAGCAGCAGGTGGTGGTGGATCAACAAAAACAAATGATGAATAGAACACCAACAGATAAGGAAGGAAATGGGAATCCAGAATGGAATCACGAGATGCGCTTTGAGTTTGATCAGTTTGATGACGATGATGATCTCTTCATTCACTTCGATCTACGACACGAGGGTGTGGGCCTGTTGGGAATCGGAGACAGGAGTATAGGAGAGGTTCGTGTGCCCTTGAAGGATCTCATACGCCTACAACAGCAGCAGGATGCATTGGCAACGTACGTAAATTATCAGGTGACGACTAGCGATAAAAAGCCCAATGGGATACTGAACTTCTCCTACAAACTACTGAATTCCAAGCTACCTTACAAATTTGAGGGTAGTACTGGAAGTCATGATGTTCATGATCATCAGCCGCTGCGTATCCAATATCCCACAATTACATTTGAAGACGATGACGATGATATATGTATTAGCAATAGCAATATTAGCAGCGCCGGCTACCACAACAAGTTCAACAGCAGTACCCAAGTCTGCAATCCGGCCAGCTCGCTAATGGAATTACATCAAACTCATACTAGTAATGCGCATTACAATTATAATCCTCCTCCGCCACCACCGGTGCTACCCCCAGCAGATGATCATGTTCATGCCTATTATCTACCTCCGCCACCGCCACCGCCACCTCCTCATCCTCCTCCTCCGCCACCGCCCATGGCAGAATATAATTATCTTCTACCTTCTCATCCAACTCGCCCGTTCCATCATCCTTACCATGATTACGGTCCTTGGAGACCAGAATTTAGACCACCGCGGCCACATGGACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

314

Amino Acids

35.71

Weight (kDa)

5.83

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 10 - 123 5.7e-09 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0024436)

Species Orthologous Gene IDs
malus_domestica MD17G1004000.v1.1
prunus_persica Prupe.3G315200_v2.0.a1
pyrus_communis pycom17g00070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 711
AarI CACCTGC 1 cut(s) 124
Acc16I TGCGCA 1 cut(s) 699
Acc36I ACCTGC 1 cut(s) 124
AccII CGCG 2 cut(s) 98, 925
AciI CCGC 9 cut(s) 527, 722, 779, 785, 791, 815, 821, 923, 925
AclWI GGATC 2 cut(s) 153, 357
AcoI YGGCCR 2 cut(s) 654, 926
AcsI RAATTY 3 cut(s) 463, 482, 911
AfaI GTAC 3 cut(s) 392, 496, 637
AgeI ACCGGT 1 cut(s) 730
AgsI TTSAA 4 cut(s) 70, 346, 560, 628
AhlI ACTAGT 1 cut(s) 689
AleI CACNNNNGTG 1 cut(s) 288
AluBI AGCT 3 cut(s) 85, 472, 660
AluI AGCT 3 cut(s) 85, 472, 660
Alw26I GTCTC 2 cut(s) 306, 898
AlwI GGATC 2 cut(s) 153, 357
AlwNI CAGNNNCTG 2 cut(s) 85, 529
AoxI GGCC 3 cut(s) 295, 654, 926
ApeKI GCWGC 4 cut(s) 130, 370, 529, 603
ApoI RAATTY 3 cut(s) 463, 482, 911
AsiGI ACCGGT 1 cut(s) 730
Asp700I GAANNNNTTC 1 cut(s) 65
AspLEI GCGC 4 cut(s) 100, 225, 608, 700
AspS9I GGNCC 2 cut(s) 295, 896
AsuHPI GGTGA 1 cut(s) 418
AvaII GGWCC 1 cut(s) 896
BaeGI GKGCMC 1 cut(s) 342
BaeI ACNNNNGTAYC 2 cut(s) 619, 652
BauI CACGAG 2 cut(s) 215, 284
BbsI GAAGAC 1 cut(s) 567
BbvI GCAGC 4 cut(s) 142, 382, 516, 615
BccI CCATC 1 cut(s) 882
BciVI GTATCC 2 cut(s) 429, 545
BclI TGATCA 3 cut(s) 235, 517, 751
BcoDI GTCTC 2 cut(s) 306, 898
BcuI ACTAGT 1 cut(s) 689
BfaI CTAG 2 cut(s) 414, 690
BfoI RGCGCY 1 cut(s) 609
BfuAI ACCTGC 1 cut(s) 124
BfuI GTATCC 2 cut(s) 429, 545
BglII AGATCT 1 cut(s) 55
BisI GCNGC 6 cut(s) 131, 371, 527, 530, 604, 926
BlsI GCNGC 6 cut(s) 132, 372, 528, 531, 605, 927
BmcAI AGTACT 1 cut(s) 496
Bme18I GGWCC 1 cut(s) 896
BmgT120I GGNCC 2 cut(s) 295, 896
BmsI GCATC 2 cut(s) 210, 367
BpiI GAAGAC 1 cut(s) 567
BsaAI YACGTR 1 cut(s) 394
BsaBI GATNNNNATC 1 cut(s) 255
BsaI GGTCTC 1 cut(s) 898
BsaJI CCNNGG 3 cut(s) 825, 899, 923
BsaWI WCCGGW 1 cut(s) 730
Bse118I RCCGGY 2 cut(s) 608, 730
Bse1I ACTGG 1 cut(s) 502
Bse8I GATNNNNATC 1 cut(s) 255
BseDI CCNNGG 3 cut(s) 825, 899, 923
BseGI GGATG 5 cut(s) 124, 382, 802, 856, 877
BseJI GATNNNNATC 1 cut(s) 255
BseNI ACTGG 1 cut(s) 502
BseRI GAGGAG 5 cut(s) 30, 708, 789, 798, 801
BseSI GKGCMC 1 cut(s) 342
BseXI GCAGC 4 cut(s) 142, 382, 516, 615
BseYI CCCAGC 1 cut(s) 742
Bsh1236I CGCG 2 cut(s) 98, 925
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 3 cut(s) 297, 656, 928
BshTI ACCGGT 1 cut(s) 730
BsiEI CGRYCG 1 cut(s) 27
BsiSI CCGG 3 cut(s) 609, 653, 731
BsiWI CGTACG 1 cut(s) 390
BsmAI GTCTC 2 cut(s) 306, 898
BsnI GGCC 3 cut(s) 297, 656, 928
Bso31I GGTCTC 1 cut(s) 898
Bsp1286I GDGCHC 1 cut(s) 342
Bsp143I GATC 9 cut(s) 24, 55, 145, 235, 256, 274, 349, 517, 751
Bsp19I CCATGG 1 cut(s) 825
BspACI CCGC 9 cut(s) 527, 722, 779, 785, 791, 815, 821, 923, 925
BspANI GGCC 3 cut(s) 297, 656, 928
BspFNI CGCG 2 cut(s) 98, 925
BspHI TCATGA 2 cut(s) 505, 514
BspMI ACCTGC 1 cut(s) 124
BspPI GGATC 2 cut(s) 153, 357
BspTNI GGTCTC 1 cut(s) 898
BsrFI RCCGGY 2 cut(s) 608, 730
BsrI ACTGG 1 cut(s) 502
BssAI RCCGGY 2 cut(s) 608, 730
BssECI CCNNGG 3 cut(s) 825, 899, 923
BssMI GATC 9 cut(s) 24, 55, 145, 235, 256, 274, 349, 517, 751
BssSI CACGAG 2 cut(s) 215, 284
BssT1I CCWWGG 2 cut(s) 825, 899
Bst2BI CACGAG 2 cut(s) 215, 284
Bst4CI ACNGT 1 cut(s) 896
Bst6I CTCTTC 1 cut(s) 266
BstBAI YACGTR 1 cut(s) 394
BstC8I GCNNGC 3 cut(s) 610, 658, 662
BstDSI CCRYGG 2 cut(s) 825, 923
BstF5I GGATG 5 cut(s) 124, 382, 802, 856, 877
BstFNI CGCG 2 cut(s) 98, 925
BstH2I RGCGCY 1 cut(s) 609
BstHHI GCGC 4 cut(s) 100, 225, 608, 700
BstKTI GATC 9 cut(s) 27, 58, 148, 238, 259, 277, 352, 520, 754
BstMAI GTCTC 2 cut(s) 306, 898
BstMBI GATC 9 cut(s) 24, 55, 145, 235, 256, 274, 349, 517, 751
BstMCI CGRYCG 1 cut(s) 27
BstMWI GCNNNNNNNGC 2 cut(s) 600, 609
BstSLI GKGCMC 1 cut(s) 342
BstSNI TACGTA 1 cut(s) 394
BstUI CGCG 2 cut(s) 98, 925
BstV1I GCAGC 4 cut(s) 142, 382, 516, 615
BstV2I GAAGAC 1 cut(s) 567
BstX2I RGATCY 2 cut(s) 55, 349
BstYI RGATCY 2 cut(s) 55, 349
BsuI GTATCC 2 cut(s) 429, 545
BsuRI GGCC 3 cut(s) 297, 656, 928
BtgI CCRYGG 2 cut(s) 825, 923
BtsCI GGATG 5 cut(s) 124, 382, 802, 856, 877
BveI ACCTGC 1 cut(s) 124
Cac8I GCNNGC 3 cut(s) 610, 658, 662
CaiI CAGNNNCTG 2 cut(s) 85, 529
CciI TCATGA 2 cut(s) 505, 514
CfoI GCGC 4 cut(s) 100, 225, 608, 700
Cfr10I RCCGGY 2 cut(s) 608, 730
Cfr13I GGNCC 2 cut(s) 295, 896
Cfr42I CCGCGG 1 cut(s) 926
Csp6I GTAC 3 cut(s) 391, 495, 636
CspAI ACCGGT 1 cut(s) 730
CviAII CATG 7 cut(s) 506, 515, 755, 761, 826, 887, 932
CviQI GTAC 3 cut(s) 391, 495, 636
DpnI GATC 9 cut(s) 26, 57, 147, 237, 258, 276, 351, 519, 753
DpnII GATC 9 cut(s) 24, 55, 145, 235, 256, 274, 349, 517, 751
EaeI YGGCCR 2 cut(s) 654, 926
Eam1104I CTCTTC 1 cut(s) 266
EarI CTCTTC 1 cut(s) 266
EciI GGCGGA 3 cut(s) 711, 768, 804
Eco105I TACGTA 1 cut(s) 394
Eco130I CCWWGG 2 cut(s) 825, 899
Eco31I GGTCTC 1 cut(s) 898
Eco47I GGWCC 1 cut(s) 896
EcoRI GAATTC 1 cut(s) 463
EcoT14I CCWWGG 2 cut(s) 825, 899
EcoT22I ATGCAT 1 cut(s) 382
ErhI CCWWGG 2 cut(s) 825, 899
FaeI CATG 7 cut(s) 509, 518, 758, 764, 829, 890, 935
FatI CATG 7 cut(s) 505, 514, 754, 760, 825, 886, 931
FbaI TGATCA 3 cut(s) 235, 517, 751
Fnu4HI GCNGC 6 cut(s) 131, 371, 527, 530, 604, 926
FokI GGATG 5 cut(s) 131, 389, 789, 843, 864
Fsp4HI GCNGC 6 cut(s) 131, 371, 527, 530, 604, 926
FspAI RTGCGCAY 1 cut(s) 699
FspBI CTAG 2 cut(s) 414, 690
FspI TGCGCA 1 cut(s) 699
GlaI GCGC 4 cut(s) 99, 224, 607, 699
GluI GCNGC 6 cut(s) 131, 371, 527, 530, 604, 926
GsaI CCCAGC 1 cut(s) 746
HaeII RGCGCY 1 cut(s) 609
HaeIII GGCC 3 cut(s) 297, 656, 928
HapII CCGG 3 cut(s) 609, 653, 731
HhaI GCGC 4 cut(s) 100, 225, 608, 700
Hin1II CATG 7 cut(s) 509, 518, 758, 764, 829, 890, 935
Hin6I GCGC 4 cut(s) 98, 223, 606, 698
HinP1I GCGC 4 cut(s) 98, 223, 606, 698
HinfI GANTC 4 cut(s) 5, 199, 211, 306
HpaII CCGG 3 cut(s) 609, 653, 731
HphI GGTGA 1 cut(s) 418
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 2 cut(s) 60, 311
Hpy188III TCNNGA 5 cut(s) 79, 203, 215, 506, 515
Hpy8I GTNNAC 1 cut(s) 94
HpyAV CCTTC 4 cut(s) 76, 182, 340, 861
HpyCH4III ACNGT 1 cut(s) 896
HpyCH4IV ACGT 2 cut(s) 389, 393
HpyCH4V TGCA 4 cut(s) 51, 112, 380, 648
HpyF10VI GCNNNNNNNGC 2 cut(s) 600, 609
HpySE526I ACGT 2 cut(s) 389, 393
Hsp92II CATG 7 cut(s) 509, 518, 758, 764, 829, 890, 935
HspAI GCGC 4 cut(s) 98, 223, 606, 698
KroI GCCGGC 1 cut(s) 608
KroNI GCCGGC 1 cut(s) 610
Ksp22I TGATCA 3 cut(s) 235, 517, 751
KspI CCGCGG 1 cut(s) 926
Kzo9I GATC 9 cut(s) 24, 55, 145, 235, 256, 274, 349, 517, 751
Lsp1109I GCAGC 4 cut(s) 142, 382, 516, 615
LweI GCATC 2 cut(s) 210, 367
MaeI CTAG 2 cut(s) 414, 690
MaeII ACGT 2 cut(s) 389, 393
MaeIII GTNAC 1 cut(s) 406
MalI GATC 9 cut(s) 26, 57, 147, 237, 258, 276, 351, 519, 753
MboI GATC 9 cut(s) 24, 55, 145, 235, 256, 274, 349, 517, 751
MboII GAAGA 4 cut(s) 67, 253, 572, 836
MflI RGATCY 2 cut(s) 55, 349
MhlI GDGCHC 1 cut(s) 342
MluCI AATT 9 cut(s) 70, 397, 463, 482, 549, 671, 706, 838, 911
MmeI TCCRAC 1 cut(s) 884
Mph1103I ATGCAT 1 cut(s) 382
MroNI GCCGGC 1 cut(s) 608
MroXI GAANNNNTTC 1 cut(s) 65
MslI CAYNNNNRTG 2 cut(s) 288, 759
MspA1I CMGCKG 2 cut(s) 529, 925
MspI CCGG 3 cut(s) 609, 653, 731
MvnI CGCG 2 cut(s) 98, 925
MwoI GCNNNNNNNGC 2 cut(s) 600, 609
NaeI GCCGGC 1 cut(s) 610
NcoI CCATGG 1 cut(s) 825
NdeII GATC 9 cut(s) 24, 55, 145, 235, 256, 274, 349, 517, 751
NgoMIV GCCGGC 1 cut(s) 608
NlaIII CATG 7 cut(s) 509, 518, 758, 764, 829, 890, 935
NmuCI GTSAC 1 cut(s) 406
NsbI TGCGCA 1 cut(s) 699
NsiI ATGCAT 1 cut(s) 382
OliI CACNNNNGTG 1 cut(s) 288
PagI TCATGA 2 cut(s) 505, 514
PaqCI CACCTGC 1 cut(s) 124
PdiI GCCGGC 1 cut(s) 610
PdmI GAANNNNTTC 1 cut(s) 65
PfeI GAWTC 4 cut(s) 5, 199, 211, 306
Pfl23II CGTACG 1 cut(s) 390
PinAI ACCGGT 1 cut(s) 730
PkrI GCNGC 6 cut(s) 132, 372, 528, 531, 605, 927
Ple19I CGATCG 1 cut(s) 27
Ppu21I YACGTR 1 cut(s) 394
PsiI TTATAA 1 cut(s) 711
PspFI CCCAGC 1 cut(s) 742
PspLI CGTACG 1 cut(s) 390
PspPI GGNCC 2 cut(s) 295, 896
PsrI GAACNNNNNNTAC 2 cut(s) 434, 466
PstNI CAGNNNCTG 2 cut(s) 85, 529
PsuI RGATCY 2 cut(s) 55, 349
PvuI CGATCG 1 cut(s) 27
RsaI GTAC 3 cut(s) 392, 496, 637
RsaNI GTAC 3 cut(s) 391, 495, 636
RseI CAYNNNNRTG 2 cut(s) 288, 759
SacII CCGCGG 1 cut(s) 926
SatI GCNGC 6 cut(s) 131, 371, 527, 530, 604, 926
Sau3AI GATC 9 cut(s) 24, 55, 145, 235, 256, 274, 349, 517, 751
Sau96I GGNCC 2 cut(s) 295, 896
ScaI AGTACT 1 cut(s) 496
SduI GDGCHC 1 cut(s) 342
SfaNI GCATC 2 cut(s) 210, 367
Sfr303I CCGCGG 1 cut(s) 926
SgrAI CRCCGGYG 1 cut(s) 730
SgrBI CCGCGG 1 cut(s) 926
SinI GGWCC 1 cut(s) 896
SmiMI CAYNNNNRTG 2 cut(s) 288, 759
SnaBI TACGTA 1 cut(s) 394
SpeI ACTAGT 1 cut(s) 689
Sse9I AATT 9 cut(s) 70, 397, 463, 482, 549, 671, 706, 838, 911
SsiI CCGC 9 cut(s) 527, 722, 779, 785, 791, 815, 821, 923, 925
SspI AATATT 1 cut(s) 598
SspMI CTAG 2 cut(s) 414, 690
StyI CCWWGG 2 cut(s) 825, 899
TaaI ACNGT 1 cut(s) 896
TaiI ACGT 2 cut(s) 392, 396
TaqI TCGA 1 cut(s) 273
TasI AATT 9 cut(s) 70, 397, 463, 482, 549, 671, 706, 838, 911
TatI WGTACW 1 cut(s) 494
TauI GCSGC 2 cut(s) 529, 928
TfiI GAWTC 4 cut(s) 5, 199, 211, 306
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 4 cut(s) 130, 370, 529, 603
Tsp45I GTSAC 1 cut(s) 406
TspDTI ATGAA 4 cut(s) 179, 253, 503, 749
VpaK11BI GGWCC 1 cut(s) 896
XapI RAATTY 3 cut(s) 463, 482, 911
XcmI CCANNNNNNNNNTGG 1 cut(s) 664
XmnI GAANNNNTTC 1 cut(s) 65
XspI CTAG 2 cut(s) 414, 690
ZrmI AGTACT 1 cut(s) 496
Zsp2I ATGCAT 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.