Prupe.3G315200_v2.0.a1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0024436
Physical Location & Seq
Forward (+)
27221830 .. 27223799
1970 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G315200.1

Sequence Viewer

Length: 1116 bp
ATGGAGCATAGATCAATGGAGTATTCCAGCAGACAACGTTATTGGGTGTTAGAAATCAAACTCATGTCGTGCAAAGATCTGAAAGCCTTCAACTTCTTTCAGAAACTCTCAGTTTATGCGATTGTGTCGCTGAAGGTAATTAGCGATGATGCGGAGAAGAAAGTAAAAGAAGCAGAAGAGCAGATGAATGAAGAGGTAGTGGAGCAACAAATTAAAAAAACACCAACTGATGGGGAAGGAGATGGAAATCCTGAATGGAATCACGAGATGCGGTTTGAGTTTGATCATAATGATGATCATCGTCAGTTGAAGGATGGACGGCTCTTGATTCACTTTGATCTGCGGCATCAGGGTCTGGGAATATTGGGAATCGGAGACAGAAGTATAGGAGAGGTTCGTGTTCCCTTGAATGATCTGATGATCCCACATATCCCACCGTCGCCGGCGAATAATTCTGGACTTGGACTTGGACTTGGAGGAGTGCAGCAGCAGCAGCAGCAGGCAAGGTATGTGAATTATCAGGTCCGGACTAGCGATGAAAAACCCAATGGGATATTGACCTTCTCCTACAAGCTGAAGCTACTCATTAAGGGTGCAGGTACTGATGATCCGCGTATTCAATACCCCACAATTACTTTGGAAGAAGATGACGATGATGATATTATACACAAGTTCAACAACACTAATTCTGATATTACGAGTTCCGGATCCCAGCAGCAGCAGGAATATTATTACACATCAAACATATCATCAGCTGCGCCCGCGCCCGCACATAATAATAATTATTTGCAATCACAATCACAGTTACAAGCTGATCATCAACAAATATGCAGCAGCTGCCTACCACAATATCATCATCCTTATGATCCACTAGTAGCAGTAGCAGTAGCAGGTCATCGCACCTATCATCCACCGCCACCACCACCGCCACCTCCCCCGCTCATGGCGGATCATATGGCATCTTACCCTTACCACCCTCCTCCACCCGGCCATGGGATGGTCCATCCTCCATGGCCACCCCATCCTTACCATGATTACGGTCCTTGGAGCCCGCCAGCTGGGATTCGACGACGCCCACATGGAGATGGACATGGACATGAACATGGGTTTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

372

Amino Acids

42.04

Weight (kDa)

5.9

Isoelectric Point (pI)

55.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024436)

Species Orthologous Gene IDs
malus_domestica MD17G1004000.v1.1
prunus_persica Prupe.3G315200_v2.0.a1
pyrus_communis pycom17g00070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 587, 881
AccB7I CCANNNNNTGG 2 cut(s) 230, 997
AccBSI CCGCTC 1 cut(s) 940
AccII CGCG 2 cut(s) 613, 764
AccIII TCCGGA 2 cut(s) 525, 704
AclI AACGTT 1 cut(s) 37
AclWI GGATC 6 cut(s) 415, 602, 702, 715, 860, 957
AcoI YGGCCR 2 cut(s) 988, 1013
AcuI CTGAAG 2 cut(s) 152, 596
AcyI GRCGYC 1 cut(s) 1072
AfaI GTAC 1 cut(s) 601
AfiI CCNNNNNNNGG 7 cut(s) 230, 943, 986, 992, 993, 997, 1058
AgsI TTSAA 5 cut(s) 91, 310, 409, 620, 676
AhlI ACTAGT 1 cut(s) 871
AluBI AGCT 6 cut(s) 574, 580, 755, 812, 837, 1058
AluI AGCT 6 cut(s) 574, 580, 755, 812, 837, 1058
Alw26I GTCTC 1 cut(s) 369
AlwI GGATC 6 cut(s) 415, 602, 702, 715, 860, 957
AlwNI CAGNNNCTG 3 cut(s) 355, 602, 837
Aor13HI TCCGGA 2 cut(s) 525, 704
AoxI GGCC 2 cut(s) 988, 1013
Asp700I GAANNNNTTC 1 cut(s) 86
AspLEI GCGC 2 cut(s) 760, 766
AspS9I GGNCC 3 cut(s) 523, 1000, 1040
AsuC2I CCSGG 1 cut(s) 987
AvaII GGWCC 3 cut(s) 523, 1000, 1040
BalI TGGCCA 1 cut(s) 1015
BamHI GGATCC 1 cut(s) 707
BanII GRGCYC 1 cut(s) 1052
BauI CACGAG 1 cut(s) 263
BccI CCATC 7 cut(s) 224, 236, 308, 991, 1011, 1029, 1079
BceAI ACGGC 1 cut(s) 335
BclI TGATCA 3 cut(s) 283, 295, 814
BcnI CCSGG 1 cut(s) 987
BcoDI GTCTC 1 cut(s) 369
BcuI ACTAGT 1 cut(s) 871
BfaI CTAG 2 cut(s) 531, 872
BfuAI ACCTGC 2 cut(s) 587, 881
BglII AGATCT 1 cut(s) 76
Bme1390I CCNGG 1 cut(s) 987
Bme18I GGWCC 3 cut(s) 523, 1000, 1040
BmgT120I GGNCC 3 cut(s) 523, 1000, 1040
BmiI GGNNCC 2 cut(s) 709, 1049
BmrFI CCNGG 1 cut(s) 987
BmsI GCATC 4 cut(s) 139, 258, 355, 968
BpuMI CCSGG 1 cut(s) 987
BsaBI GATNNNNATC 2 cut(s) 246, 297
BsaHI GRCGYC 1 cut(s) 1072
BsaJI CCNNGG 3 cut(s) 991, 1010, 1043
BsaWI WCCGGW 2 cut(s) 525, 704
Bsc4I CCNNNNNNNGG 7 cut(s) 230, 943, 986, 992, 993, 997, 1058
Bse118I RCCGGY 1 cut(s) 442
Bse8I GATNNNNATC 2 cut(s) 246, 297
BseAI TCCGGA 2 cut(s) 525, 704
BseDI CCNNGG 3 cut(s) 991, 1010, 1043
BseGI GGATG 6 cut(s) 319, 856, 907, 1002, 1003, 1021
BseJI GATNNNNATC 2 cut(s) 246, 297
BseLI CCNNNNNNNGG 7 cut(s) 230, 943, 986, 992, 993, 997, 1058
BseMII CTCAG 1 cut(s) 123
BseRI GAGGAG 2 cut(s) 492, 969
BseYI CCCAGC 2 cut(s) 711, 1058
BsgI GTGCAG 2 cut(s) 503, 615
Bsh1236I CGCG 2 cut(s) 613, 764
BshFI GGCC 2 cut(s) 990, 1015
BsiSI CCGG 4 cut(s) 443, 526, 705, 987
BslI CCNNNNNNNGG 7 cut(s) 230, 943, 986, 992, 993, 997, 1058
BsmAI GTCTC 1 cut(s) 369
BsnI GGCC 2 cut(s) 990, 1015
Bsp1286I GDGCHC 1 cut(s) 1052
Bsp13I TCCGGA 2 cut(s) 525, 704
Bsp19I CCATGG 2 cut(s) 991, 1010
BspANI GGCC 2 cut(s) 990, 1015
BspCNI CTCAG 1 cut(s) 122
BspEI TCCGGA 2 cut(s) 525, 704
BspFNI CGCG 2 cut(s) 613, 764
BspLI GGNNCC 2 cut(s) 709, 1049
BspMI ACCTGC 2 cut(s) 587, 881
BspPI GGATC 6 cut(s) 415, 602, 702, 715, 860, 957
BspQI GCTCTTC 1 cut(s) 171
BsrBI CCGCTC 1 cut(s) 940
BsrFI RCCGGY 1 cut(s) 442
BssAI RCCGGY 1 cut(s) 442
BssECI CCNNGG 3 cut(s) 991, 1010, 1043
BssNI GRCGYC 1 cut(s) 1072
BssSI CACGAG 1 cut(s) 263
BssT1I CCWWGG 3 cut(s) 991, 1010, 1043
Bst2BI CACGAG 1 cut(s) 263
Bst4CI ACNGT 3 cut(s) 438, 804, 1040
Bst6I CTCTTC 2 cut(s) 171, 186
BstACI GRCGYC 1 cut(s) 1072
BstAPI GCANNNNNTGC 1 cut(s) 837
BstC8I GCNNGC 6 cut(s) 444, 501, 762, 768, 1052, 1056
BstDEI CTNAG 1 cut(s) 109
BstDSI CCRYGG 2 cut(s) 991, 1010
BstF5I GGATG 6 cut(s) 319, 856, 907, 1002, 1003, 1021
BstFNI CGCG 2 cut(s) 613, 764
BstHHI GCGC 2 cut(s) 760, 766
BstMAI GTCTC 1 cut(s) 369
BstMWI GCNNNNNNNGC 5 cut(s) 490, 493, 496, 761, 837
BstSCI CCNGG 1 cut(s) 985
BstUI CGCG 2 cut(s) 613, 764
BstX2I RGATCY 2 cut(s) 76, 707
BstYI RGATCY 2 cut(s) 76, 707
BsuRI GGCC 2 cut(s) 990, 1015
BtgI CCRYGG 2 cut(s) 991, 1010
BtgZI GCGATG 3 cut(s) 159, 549, 881
BtsCI GGATG 6 cut(s) 319, 856, 907, 1002, 1003, 1021
BveI ACCTGC 2 cut(s) 587, 881
Cac8I GCNNGC 6 cut(s) 444, 501, 762, 768, 1052, 1056
CaiI CAGNNNCTG 3 cut(s) 355, 602, 837
CfoI GCGC 2 cut(s) 760, 766
Cfr10I RCCGGY 1 cut(s) 442
Cfr13I GGNCC 3 cut(s) 523, 1000, 1040
CseI GACGC 1 cut(s) 1080
Csp6I GTAC 1 cut(s) 600
CviAII CATG 9 cut(s) 64, 943, 992, 1011, 1031, 1079, 1091, 1097, 1103
CviQI GTAC 1 cut(s) 600
DdeI CTNAG 1 cut(s) 109
EaeI YGGCCR 2 cut(s) 988, 1013
Eam1104I CTCTTC 2 cut(s) 171, 186
EarI CTCTTC 2 cut(s) 171, 186
EciI GGCGGA 1 cut(s) 962
Eco130I CCWWGG 3 cut(s) 991, 1010, 1043
Eco24I GRGCYC 1 cut(s) 1052
Eco47I GGWCC 3 cut(s) 523, 1000, 1040
Eco57I CTGAAG 2 cut(s) 152, 596
EcoT14I CCWWGG 3 cut(s) 991, 1010, 1043
EcoT38I GRGCYC 1 cut(s) 1052
ErhI CCWWGG 3 cut(s) 991, 1010, 1043
FaeI CATG 9 cut(s) 67, 946, 995, 1014, 1034, 1082, 1094, 1100, 1106
FatI CATG 9 cut(s) 63, 942, 991, 1010, 1030, 1078, 1090, 1096, 1102
FauI CCCGC 4 cut(s) 769, 775, 945, 1059
FauNDI CATATG 1 cut(s) 954
FbaI TGATCA 3 cut(s) 283, 295, 814
FokI GGATG 6 cut(s) 326, 843, 894, 990, 1008, 1009
FriOI GRGCYC 1 cut(s) 1052
FspBI CTAG 2 cut(s) 531, 872
GlaI GCGC 2 cut(s) 759, 765
GsaI CCCAGC 2 cut(s) 715, 1062
HaeIII GGCC 2 cut(s) 990, 1015
HapII CCGG 4 cut(s) 443, 526, 705, 987
HgaI GACGC 1 cut(s) 1080
HhaI GCGC 2 cut(s) 760, 766
Hin1I GRCGYC 1 cut(s) 1072
Hin1II CATG 9 cut(s) 67, 946, 995, 1014, 1034, 1082, 1094, 1100, 1106
Hin6I GCGC 2 cut(s) 758, 764
HinP1I GCGC 2 cut(s) 758, 764
HinfI GANTC 4 cut(s) 259, 328, 369, 1063
HpaII CCGG 4 cut(s) 443, 526, 705, 987
Hpy188I TCNGA 5 cut(s) 81, 102, 374, 417, 691
Hpy188III TCNNGA 6 cut(s) 251, 263, 325, 456, 526, 705
Hpy99I CGWCG 3 cut(s) 442, 1071, 1074
HpyAV CCTTC 5 cut(s) 97, 127, 230, 304, 571
HpyCH4III ACNGT 3 cut(s) 438, 804, 1040
HpyCH4IV ACGT 1 cut(s) 37
HpyCH4V TGCA 5 cut(s) 72, 484, 596, 790, 831
HpyF10VI GCNNNNNNNGC 5 cut(s) 490, 493, 496, 761, 837
HpyF3I CTNAG 1 cut(s) 109
HpySE526I ACGT 1 cut(s) 37
Hsp92I GRCGYC 1 cut(s) 1072
Hsp92II CATG 9 cut(s) 67, 946, 995, 1014, 1034, 1082, 1094, 1100, 1106
HspAI GCGC 2 cut(s) 758, 764
Kpn2I TCCGGA 2 cut(s) 525, 704
KroI GCCGGC 1 cut(s) 442
KroNI GCCGGC 1 cut(s) 444
Ksp22I TGATCA 3 cut(s) 283, 295, 814
LguI GCTCTTC 1 cut(s) 171
LmnI GCTCC 3 cut(s) 4, 202, 1047
LweI GCATC 4 cut(s) 139, 258, 355, 968
MaeI CTAG 2 cut(s) 531, 872
MaeII ACGT 1 cut(s) 37
MaeIII GTNAC 1 cut(s) 804
MbiI CCGCTC 1 cut(s) 940
MboII GAAGA 5 cut(s) 169, 188, 203, 653, 656
MflI RGATCY 2 cut(s) 76, 707
MhlI GDGCHC 1 cut(s) 1052
MlsI TGGCCA 1 cut(s) 1015
MluCI AATT 7 cut(s) 138, 210, 451, 514, 630, 685, 781
MluNI TGGCCA 1 cut(s) 1015
MnlI CCTC 7 cut(s) 187, 385, 470, 942, 987, 990, 1017
Mox20I TGGCCA 1 cut(s) 1015
MreI CGCCGGCG 1 cut(s) 442
MroI TCCGGA 2 cut(s) 525, 704
MroNI GCCGGC 1 cut(s) 442
MroXI GAANNNNTTC 1 cut(s) 86
MscI TGGCCA 1 cut(s) 1015
MseI TTAA 3 cut(s) 213, 588, 1114
MslI CAYNNNNRTG 5 cut(s) 291, 861, 1083, 1095, 1101
Msp20I TGGCCA 1 cut(s) 1015
MspA1I CMGCKG 3 cut(s) 755, 837, 1058
MspI CCGG 4 cut(s) 443, 526, 705, 987
MspR9I CCNGG 1 cut(s) 987
MvnI CGCG 2 cut(s) 613, 764
MwoI GCNNNNNNNGC 5 cut(s) 490, 493, 496, 761, 837
NaeI GCCGGC 1 cut(s) 444
NciI CCSGG 1 cut(s) 987
NcoI CCATGG 2 cut(s) 991, 1010
NdeI CATATG 1 cut(s) 954
NgoMIV GCCGGC 1 cut(s) 442
NlaIII CATG 9 cut(s) 67, 946, 995, 1014, 1034, 1082, 1094, 1100, 1106
NlaIV GGNNCC 2 cut(s) 709, 1049
PciSI GCTCTTC 1 cut(s) 171
PdiI GCCGGC 1 cut(s) 444
PdmI GAANNNNTTC 1 cut(s) 86
PfeI GAWTC 4 cut(s) 259, 328, 369, 1063
PflMI CCANNNNNTGG 2 cut(s) 230, 997
Psp1406I AACGTT 1 cut(s) 37
PspFI CCCAGC 2 cut(s) 711, 1058
PspN4I GGNNCC 2 cut(s) 709, 1049
PspPI GGNCC 3 cut(s) 523, 1000, 1040
PstNI CAGNNNCTG 3 cut(s) 355, 602, 837
PsuI RGATCY 2 cut(s) 76, 707
PvuII CAGCTG 3 cut(s) 755, 837, 1058
RsaI GTAC 1 cut(s) 601
RsaNI GTAC 1 cut(s) 600
RseI CAYNNNNRTG 5 cut(s) 291, 861, 1083, 1095, 1101
SapI GCTCTTC 1 cut(s) 171
SaqAI TTAA 3 cut(s) 213, 588, 1114
Sau96I GGNCC 3 cut(s) 523, 1000, 1040
ScrFI CCNGG 1 cut(s) 987
SduI GDGCHC 1 cut(s) 1052
SfaNI GCATC 4 cut(s) 139, 258, 355, 968
SgrAI CRCCGGYG 1 cut(s) 442
SinI GGWCC 3 cut(s) 523, 1000, 1040
SmiMI CAYNNNNRTG 5 cut(s) 291, 861, 1083, 1095, 1101
SpeI ACTAGT 1 cut(s) 871
Sse9I AATT 7 cut(s) 138, 210, 451, 514, 630, 685, 781
SspI AATATT 2 cut(s) 363, 728
SspMI CTAG 2 cut(s) 531, 872
StyD4I CCNGG 1 cut(s) 985
StyI CCWWGG 3 cut(s) 991, 1010, 1043
TaaI ACNGT 3 cut(s) 438, 804, 1040
TaiI ACGT 1 cut(s) 40
TaqI TCGA 1 cut(s) 1066
TasI AATT 7 cut(s) 138, 210, 451, 514, 630, 685, 781
TauI GCSGC 1 cut(s) 346
TfiI GAWTC 4 cut(s) 259, 328, 369, 1063
Tru1I TTAA 3 cut(s) 213, 588, 1114
Tru9I TTAA 3 cut(s) 213, 588, 1114
TspDTI ATGAA 4 cut(s) 200, 204, 552, 1113
Van91I CCANNNNNTGG 2 cut(s) 230, 997
VpaK11BI GGWCC 3 cut(s) 523, 1000, 1040
XcmI CCANNNNNNNNNTGG 1 cut(s) 634
XmnI GAANNNNTTC 1 cut(s) 86
XspI CTAG 2 cut(s) 531, 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.