Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0005683

Physical Location & Seq

Genomic Coordinates Forward (+)

1118674 .. 1121601

Gene Structure 2928 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1013000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2328 bp

ATGGCGGAACACTCGAACCACCATGCAGAAGCGGCGATTCCGCAAACCATGCAAGAAACCGGTACGGAAGAAAGAGATCCAGAGAGCATTTCTCTCCAGCAACCACTCCTCAAGAGAAACCGAACACTTTCTTCCACTCCACTGGCTATGGTCGGAGCCAAAGTCTCCCATATCGAGAGCTTGGACTACGAGATCAACGAGAACGATCTGTTTAAGCATGATTGGAGAAGCAGATCCAAAGTCCAAGTCTTGCAGTATGTATTTTTGAAATGGATCCTTGCCTGCCTTGTTGGACTTCTAACTGGTATAATTGCCACCCTCATTAACCTCGCTGTTGAGAATATTGCCGGCTACAAGCTTCTTGCCGCGGTTAGTTTCATAGAGAAAGAGAGGTACCTGATGGGGTTCATCTTTATAGCTGGGGCCAATCTTCTACTGACCGCTATTGCTTCTGTTCTCTGCGTGTGCTTTGCTCCGACTGCAGCTGGACCTGGTATACCTGAAATCAAAGCATATCTCAACGGAGTTGACACACCGAATATGTTTGGTGCGACAACAATGATTGTCAAGATATTCGGAAGCATTGGGGCTGTTTCTGCAGGCCTAGATCTTGGAAAAGAAGGGCCTCTTGTGCACATTGGAAGCTGCATTGCTTCCTTATTAGGTCAAGGGGGACCTGACAATTACCGTATTAAATGGTGCTGGCTCCGCTACTTCAACAATGATCGTGACCGCAGAGATCTCATTACCTGTGGCGCTGCCTCTGGGGTATGTGCAGCTTTCCGAGCTCCTGTGGGTGGTGTACTGTTTGCCCTTGAAGAGGTGGCAACATGGTGGAGGAGTGCCCTTCTCTGGAGAACTTTCTTCAGCACAGCTATTGTGGTGGTGGTGCTTAGAGCTTTTATCGAAATATGCAATTCTGGGGAATGTGGGCTTTTCGGGAAAGGAGGGCTTATCATGTTCGACGTAAGCACTGTTACTGTGACATACAATGCGTGGGATATCATCCCCGTAGCTGTAATTGGCATTATTGGTGGAGTTTTGGGAAGCCTGTACAACTATCTTCTCCACAAGATTCTCAGACTATACAACCTCATAAATCAGAAGGGGAAAGTTCTCAAGATCCTCCTCAGTCTCACTGTATCACTCTTTACTTCTGGATGCCTATACGGTCTCCCTTTCCTTGTCGAATGTACACCCTGTGACTCCTCTCTTTCGGAGTCTGCCTGTCCCACCAATGAAGGATCTGGAAACTACAAAAAGTTCAACTGCCCAGATGGCCACTACAATGACCTAGCTACTCTTCTCCTTGCCACTAATGATGATGCAGTTCGAAACATCTTTTCGACAAACACTTCTACTGAGTATCATCCTTTATCCCTCCTAATCTTCTTCGTACTATATTGCATCTTAGGATTGTTTACCTTTGGAATCGCTGTGCCGTCTGGACTGTTCCTCCCCATCATCCTTATGGGCTCAGCTTATGGCCGTCTGCTTGGAATTGCCATGCAATCATATACGAGCATTGACCAGGGGCTTTTCGCTGTTCTTGGTGCAGCTTCCCTAATGGCAGGGTCAATGAGGATGACTGTTTCGTTATGTGTCATATTTCTTGAGCTCACCAACAACCTCCTCTTACTACCCATAACAATGCTTGTCCTTCTAATTGCCAAAACTGTTGGAGACAGTTTCAATCCAAGCATCTATGAGATTATACTGCACTTAAAAGGTTTGCCTTTCTTGGAGGCACATCCAGAACCGTGGATGAGAAATCTGACTGTAGGTGAGCTCGCTGATGCCAAACTGCCAGTAGTAACCCTCCGTGGAATTGAAAAGGTGGATCGTATTGTGGAGGTCCTGAGAAACACCACGCACAATGGTTTCCCTGTTGTAGAAGAAGGAGTGGTGCCACCAATGGGATTGGCAGTTGGGGCAACAGTACATGGACTAATTCTTCGAGCTCACCTTCTTCAGGTGCTGAAAAAGAAATGGTTCCTAAGAGAGAAAAGAAGAACAGAGGAGTGGGAAGTTAGAGAGAAATTTACCTCGGTTGAGTTGGCCGAGAGGGAATGGAAGATCGAGGAGGTGGCTGTGACAAAAGATGAAATGGAGATGTATGTAGATCTGCATCCACTCACCAATAGAACACCATACACAGTGATGGAAGGCATGTCAGTGGCAAAAGCTATGGTGCTTTTCAGGCAAGTGGGACTTCGCCATTTGCTCGTTGTGCCAAATTATGAAGCATCTCGGTTTACTTCCGCACGAGCATATTTATTCTGTCGATTGAGCATAGTTATTCAAGACGGTACACTATACTTTTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0001101 GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005247 GO:0005253 GO:0005254 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006812 GO:0006820 GO:0006821 GO:0008150 GO:0008308 GO:0008324 GO:0008509 GO:0009671 GO:0009705 GO:0010035 GO:0010167 GO:0015075 GO:0015077 GO:0015078 GO:0015103 GO:0015108 GO:0015112 GO:0015267 GO:0015291 GO:0015293 GO:0015294 GO:0015295 GO:0015296 GO:0015318 GO:0015513 GO:0015672 GO:0015698 GO:0015706 GO:0016020 GO:0022803 GO:0022804 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0022890 GO:0031090 GO:0034220 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0071705 GO:0098588 GO:0098655 GO:0098656 GO:0098660 GO:0098661 GO:0098662 GO:0098805 GO:1901698 GO:1901700 GO:1902476 GO:1902600

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBS Voltage_CLC

Protein Analysis

776

Amino Acids

85.44

Weight (kDa)

6.49

Isoelectric Point (pI)

34.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Voltage_CLC	PF00654	147 - 561	7.7e-78	Voltage gated chloride channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005683)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27170 AT5G40890 AT5G40890
fragaria_vesca	FvH4_6g53240 FvH4_6g53240 FvH4_6g53240 FvH4_6g53240 FvH4_6g53240 FvH4_6g53240
malus_domestica	MD17G1013000.v1.1
prunus_persica	Prupe.3G304600_v2.0.a1 Prupe.3G304600_v2.0.a1 Prupe.3G304600_v2.0.a1 Prupe.3G304600_v2.0.a1 Prupe.3G304600_v2.0.a1 Prupe.3G304600_v2.0.a1 Prupe.3G304600_v2.0.a1
pyrus_communis	pycom111g01040 pycom17g01040
rosa_laevigata	RLG00000022324
rosa_multiflora	Rmu_sc0003512.1_g000010
rosa_roxburghii	Rroxscaffold_2G00077410
rosa_rugosa	Rorug02G0583700
rosa_samantha	Rh2AG663800 Rh2BG674600 Rh2CG637800 Rh2DG688600
rosa_wichuraiana	Rw2G054400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	393
AccB1I	GGYRCC	2 cut(s)	393, 1906
AccI	GTMKAC	1 cut(s)	496
AccII	CGCG	1 cut(s)	368
AciI	CCGC	9 cut(s)	5, 32, 41, 366, 368, 441, 709, 733, 2262
AclWI	GGATC	7 cut(s)	71, 228, 268, 281, 1117, 1252, 1848
AcoI	YGGCCR	3 cut(s)	1279, 1486, 2058
AcsI	RAATTY	1 cut(s)	2039
AcuI	CTGAAG	2 cut(s)	850, 1955
AdeI	CACNNNGTG	1 cut(s)	1202
AfaI	GTAC	8 cut(s)	64, 395, 804, 1055, 1195, 1398, 1941, 2311
AfiI	CCNNNNNNNGG	5 cut(s)	797, 820, 852, 1916, 1972
AgeI	ACCGGT	1 cut(s)	59
AgsI	TTSAA	7 cut(s)	268, 718, 818, 1267, 1693, 1832, 2303
AjnI	CCWGG	2 cut(s)	490, 1530
Alw21I	GWGCWC	5 cut(s)	636, 790, 1620, 1791, 1963
Alw26I	GTCTC	4 cut(s)	169, 1139, 1178, 1677
Alw44I	GTGCAC	1 cut(s)	632
AlwI	GGATC	7 cut(s)	71, 228, 268, 281, 1117, 1252, 1848
AlwNI	CAGNNNCTG	1 cut(s)	1978
AoxI	GGCC	6 cut(s)	423, 601, 623, 1279, 1486, 2058
ApaLI	GTGCAC	1 cut(s)	632
ApeKI	GCWGC	5 cut(s)	482, 645, 758, 776, 1556
ApoI	RAATTY	1 cut(s)	2039
ArsI	GACNNNNNNTTYG	4 cut(s)	231, 263, 1938, 1970
AsiGI	ACCGGT	1 cut(s)	59
Asp700I	GAANNNNTTC	2 cut(s)	127, 1991
Asp718I	GGTACC	1 cut(s)	393
AspLEI	GCGC	1 cut(s)	758
AspS9I	GGNCC	5 cut(s)	423, 488, 623, 674, 1855
AsuHPI	GGTGA	4 cut(s)	1612, 1796, 1955, 2128
AsuII	TTCGAA	1 cut(s)	1333
AvaII	GGWCC	3 cut(s)	488, 674, 1855
BaeGI	GKGCMC	2 cut(s)	636, 847
BalI	TGGCCA	1 cut(s)	1281
BamHI	GGATCC	1 cut(s)	273
BanI	GGYRCC	2 cut(s)	393, 1906
BanII	GRGCYC	5 cut(s)	790, 1478, 1620, 1791, 1963
BauI	CACGAG	1 cut(s)	2265
Bbv12I	GWGCWC	5 cut(s)	636, 790, 1620, 1791, 1963
BbvI	GCAGC	5 cut(s)	494, 632, 745, 788, 1568
BccI	CCATC	4 cut(s)	394, 1271, 1469, 2155
BceAI	ACGGC	2 cut(s)	1426, 1473
BciT130I	CCWGG	2 cut(s)	492, 1532
BcoDI	GTCTC	4 cut(s)	169, 1139, 1178, 1677
BfaI	CTAG	2 cut(s)	605, 1295
BfmI	CTRYAG	3 cut(s)	480, 597, 1779
BfoI	RGCGCY	1 cut(s)	759
BglI	GCCNNNNNGGC	1 cut(s)	1278
BglII	AGATCT	3 cut(s)	607, 739, 2122
BisI	GCNGC	7 cut(s)	33, 366, 483, 646, 759, 777, 1557
BlpI	GCTNAGC	1 cut(s)	1477
BlsI	GCNGC	7 cut(s)	34, 367, 484, 647, 760, 778, 1558
Bme1390I	CCNGG	2 cut(s)	492, 1532
Bme18I	GGWCC	3 cut(s)	488, 674, 1855
BmgT120I	GGNCC	5 cut(s)	423, 488, 623, 674, 1855
BmiI	GGNNCC	8 cut(s)	157, 275, 395, 424, 675, 707, 1908, 1994
BmrFI	CCNGG	2 cut(s)	492, 1532
BmsI	GCATC	7 cut(s)	1151, 1315, 1416, 1710, 1786, 2137, 2255
BplI	GAGNNNNNCTC	2 cut(s)	76, 108
BpmI	CTGGAG	2 cut(s)	80, 874
Bpu1102I	GCTNAGC	1 cut(s)	1477
Bpu14I	TTCGAA	1 cut(s)	1333
BpuEI	CTTGAG	3 cut(s)	95, 1103, 1634
BsaBI	GATNNNNATC	1 cut(s)	2127
BsaI	GGTCTC	1 cut(s)	1178
BsaJI	CCNNGG	5 cut(s)	366, 1531, 1760, 1822, 2046
BsaWI	WCCGGW	1 cut(s)	59
BsaXI	ACNNNNNCTCC	4 cut(s)	147, 177, 1440, 1470
Bsc4I	CCNNNNNNNGG	5 cut(s)	797, 820, 852, 1916, 1972
Bse118I	RCCGGY	2 cut(s)	59, 347
Bse1I	ACTGG	3 cut(s)	147, 307, 1808
Bse3DI	GCAATG	1 cut(s)	648
Bse8I	GATNNNNATC	1 cut(s)	2127
BseBI	CCWGG	2 cut(s)	492, 1532
BseDI	CCNNGG	5 cut(s)	366, 1531, 1760, 1822, 2046
BseGI	GGATG	8 cut(s)	1005, 1166, 1369, 1464, 1590, 1750, 1770, 2128
BseJI	GATNNNNATC	1 cut(s)	2127
BseLI	CCNNNNNNNGG	5 cut(s)	797, 820, 852, 1916, 1972
BseMI	GCAATG	1 cut(s)	648
BseMII	CTCAG	5 cut(s)	1093, 1144, 1353, 1491, 1850
BseNI	ACTGG	3 cut(s)	147, 307, 1808
BseRI	GAGGAG	7 cut(s)	98, 853, 1118, 1198, 1622, 2033, 2096
BseSI	GKGCMC	2 cut(s)	636, 847
BseXI	GCAGC	5 cut(s)	494, 632, 745, 788, 1568
BseYI	CCCAGC	1 cut(s)	419
BsgI	GTGCAG	3 cut(s)	795, 1575, 1703
Bsh1236I	CGCG	1 cut(s)	368
BshFI	GGCC	6 cut(s)	425, 603, 625, 1281, 1488, 2060
BshNI	GGYRCC	2 cut(s)	393, 1906
BshTI	ACCGGT	1 cut(s)	59
BsiHKAI	GWGCWC	5 cut(s)	636, 790, 1620, 1791, 1963
BsiSI	CCGG	2 cut(s)	60, 348
BslFI	GGGAC	3 cut(s)	687, 1215, 2223
BslI	CCNNNNNNNGG	5 cut(s)	797, 820, 852, 1916, 1972
BsmAI	GTCTC	4 cut(s)	169, 1139, 1178, 1677
BsmFI	GGGAC	3 cut(s)	687, 1215, 2223
BsnI	GGCC	6 cut(s)	425, 603, 625, 1281, 1488, 2060
Bso31I	GGTCTC	1 cut(s)	1178
Bsp119I	TTCGAA	1 cut(s)	1333
Bsp1286I	GDGCHC	7 cut(s)	636, 790, 847, 1478, 1620, 1791, 1963
Bsp1407I	TGTACA	2 cut(s)	1053, 1193
Bsp1720I	GCTNAGC	1 cut(s)	1477
BspACI	CCGC	9 cut(s)	5, 32, 41, 366, 368, 441, 709, 733, 2262
BspANI	GGCC	6 cut(s)	425, 603, 625, 1281, 1488, 2060
BspCNI	CTCAG	5 cut(s)	1092, 1143, 1354, 1490, 1851
BspFNI	CGCG	1 cut(s)	368
BspLI	GGNNCC	8 cut(s)	157, 275, 395, 424, 675, 707, 1908, 1994
BspMAI	CTGCAG	2 cut(s)	484, 601
BspPI	GGATC	7 cut(s)	71, 228, 268, 281, 1117, 1252, 1848
BspT104I	TTCGAA	1 cut(s)	1333
BspT107I	GGYRCC	2 cut(s)	393, 1906
BspTNI	GGTCTC	1 cut(s)	1178
BsrDI	GCAATG	1 cut(s)	648
BsrFI	RCCGGY	2 cut(s)	59, 347
BsrGI	TGTACA	2 cut(s)	1053, 1193
BsrI	ACTGG	3 cut(s)	147, 307, 1808
BssAI	RCCGGY	2 cut(s)	59, 347
BssECI	CCNNGG	5 cut(s)	366, 1531, 1760, 1822, 2046
BssNAI	GTATAC	1 cut(s)	497
BssSI	CACGAG	1 cut(s)	2265
Bst1107I	GTATAC	1 cut(s)	497
Bst2BI	CACGAG	1 cut(s)	2265
Bst2UI	CCWGG	2 cut(s)	492, 1532
Bst6I	CTCTTC	2 cut(s)	813, 1308
BstAPI	GCANNNNNTGC	1 cut(s)	49
BstAUI	TGTACA	2 cut(s)	1053, 1193
BstBI	TTCGAA	1 cut(s)	1333
BstC8I	GCNNGC	5 cut(s)	283, 349, 601, 704, 1791
BstDEI	CTNAG	8 cut(s)	893, 1079, 1130, 1362, 1411, 1477, 1859, 1997
BstDSI	CCRYGG	3 cut(s)	366, 1760, 1822
BstENI	CCTNNNNNAGG	2 cut(s)	818, 1970
BstF5I	GGATG	8 cut(s)	1005, 1166, 1369, 1464, 1590, 1750, 1770, 2128
BstFNI	CGCG	1 cut(s)	368
BstH2I	RGCGCY	1 cut(s)	759
BstHHI	GCGC	1 cut(s)	758
BstMAI	GTCTC	4 cut(s)	169, 1139, 1178, 1677
BstNI	CCWGG	2 cut(s)	492, 1532
BstNSI	RCATGY	1 cut(s)	2173
BstSCI	CCNGG	2 cut(s)	490, 1530
BstSFI	CTRYAG	3 cut(s)	480, 597, 1779
BstSLI	GKGCMC	2 cut(s)	636, 847
BstUI	CGCG	1 cut(s)	368
BstV1I	GCAGC	5 cut(s)	494, 632, 745, 788, 1568
BstX2I	RGATCY	8 cut(s)	76, 233, 273, 607, 739, 1122, 1244, 2122
BstXI	CCANNNNNNTGG	2 cut(s)	142, 1761
BstYI	RGATCY	8 cut(s)	76, 233, 273, 607, 739, 1122, 1244, 2122
BstZ17I	GTATAC	1 cut(s)	497
BsuRI	GGCC	6 cut(s)	425, 603, 625, 1281, 1488, 2060
BtgI	CCRYGG	3 cut(s)	366, 1760, 1822
BtsCI	GGATG	8 cut(s)	1005, 1166, 1369, 1464, 1590, 1750, 1770, 2128
BtsIMutI	CAGTG	5 cut(s)	140, 972, 1137, 2163, 2181
Cac8I	GCNNGC	5 cut(s)	283, 349, 601, 704, 1791
CaiI	CAGNNNCTG	1 cut(s)	1978
CfoI	GCGC	1 cut(s)	758
Cfr10I	RCCGGY	2 cut(s)	59, 347
Cfr13I	GGNCC	5 cut(s)	423, 488, 623, 674, 1855
Cfr42I	CCGCGG	1 cut(s)	369
CsiI	ACCWGGT	1 cut(s)	490
Csp6I	GTAC	8 cut(s)	63, 394, 803, 1054, 1194, 1397, 1940, 2310
CspAI	ACCGGT	1 cut(s)	59
CviAII	CATG	8 cut(s)	23, 49, 218, 831, 958, 1507, 1943, 2170
CviQI	GTAC	8 cut(s)	63, 394, 803, 1054, 1194, 1397, 1940, 2310
DdeI	CTNAG	8 cut(s)	893, 1079, 1130, 1362, 1411, 1477, 1859, 1997
DraIII	CACNNNGTG	1 cut(s)	1202
EaeI	YGGCCR	3 cut(s)	1279, 1486, 2058
Eam1104I	CTCTTC	2 cut(s)	813, 1308
EarI	CTCTTC	2 cut(s)	813, 1308
EciI	GGCGGA	1 cut(s)	20
Ecl136II	GAGCTC	4 cut(s)	788, 1618, 1789, 1961
Eco147I	AGGCCT	1 cut(s)	603
Eco24I	GRGCYC	5 cut(s)	790, 1478, 1620, 1791, 1963
Eco31I	GGTCTC	1 cut(s)	1178
Eco32I	GATATC	1 cut(s)	1003
Eco47I	GGWCC	3 cut(s)	488, 674, 1855
Eco53kI	GAGCTC	4 cut(s)	788, 1618, 1789, 1961
Eco57I	CTGAAG	2 cut(s)	850, 1955
EcoICRI	GAGCTC	4 cut(s)	788, 1618, 1789, 1961
EcoNI	CCTNNNNNAGG	2 cut(s)	818, 1970
EcoO109I	RGGNCCY	3 cut(s)	623, 674, 1855
EcoRII	CCWGG	2 cut(s)	490, 1530
EcoRV	GATATC	1 cut(s)	1003
EcoT38I	GRGCYC	5 cut(s)	790, 1478, 1620, 1791, 1963
FaeI	CATG	8 cut(s)	26, 52, 221, 834, 961, 1510, 1946, 2173
FalI	AAGNNNNNCTT	6 cut(s)	612, 644, 936, 968, 2196, 2228
FaqI	GGGAC	3 cut(s)	687, 1215, 2223
FatI	CATG	8 cut(s)	22, 48, 217, 830, 957, 1506, 1942, 2169
FblI	GTMKAC	1 cut(s)	496
Fnu4HI	GCNGC	7 cut(s)	33, 366, 483, 646, 759, 777, 1557
FokI	GGATG	8 cut(s)	992, 1173, 1356, 1451, 1597, 1737, 1777, 2115
FriOI	GRGCYC	5 cut(s)	790, 1478, 1620, 1791, 1963
Fsp4HI	GCNGC	7 cut(s)	33, 366, 483, 646, 759, 777, 1557
FspBI	CTAG	2 cut(s)	605, 1295
GlaI	GCGC	1 cut(s)	757
GluI	GCNGC	7 cut(s)	33, 366, 483, 646, 759, 777, 1557
GsaI	CCCAGC	1 cut(s)	423
GsuI	CTGGAG	2 cut(s)	80, 874
HaeII	RGCGCY	1 cut(s)	759
HaeIII	GGCC	6 cut(s)	425, 603, 625, 1281, 1488, 2060
HapII	CCGG	2 cut(s)	60, 348
HhaI	GCGC	1 cut(s)	758
Hin1II	CATG	8 cut(s)	26, 52, 221, 834, 961, 1510, 1946, 2173
Hin6I	GCGC	1 cut(s)	756
HinP1I	GCGC	1 cut(s)	756
HincII	GTYRAC	1 cut(s)	529
HindII	GTYRAC	1 cut(s)	529
HindIII	AAGCTT	1 cut(s)	356
HinfI	GANTC	5 cut(s)	37, 1075, 1205, 1220, 1431
HpaII	CCGG	2 cut(s)	60, 348
HphI	GGTGA	4 cut(s)	1612, 1796, 1955, 2128
Hpy166II	GTNNAC	8 cut(s)	497, 529, 634, 803, 1196, 1422, 2256, 2312
Hpy188I	TCNGA	8 cut(s)	155, 477, 578, 785, 1082, 1104, 1219, 1776
Hpy8I	GTNNAC	8 cut(s)	497, 529, 634, 803, 1196, 1422, 2256, 2312
Hpy99I	CGWCG	1 cut(s)	968
HpyAV	CCTTC	8 cut(s)	614, 857, 1099, 1235, 1670, 1892, 1976, 2159
HpyCH4IV	ACGT	1 cut(s)	966
HpyF3I	CTNAG	8 cut(s)	893, 1079, 1130, 1362, 1411, 1477, 1859, 1997
HpySE526I	ACGT	1 cut(s)	966
Hsp92II	CATG	8 cut(s)	26, 52, 221, 834, 961, 1510, 1946, 2173
HspAI	GCGC	1 cut(s)	756
KpnI	GGTACC	1 cut(s)	397
KroI	GCCGGC	1 cut(s)	347
KroNI	GCCGGC	1 cut(s)	349
KspI	CCGCGG	1 cut(s)	369
LmnI	GCTCC	4 cut(s)	155, 478, 711, 793
Lsp1109I	GCAGC	5 cut(s)	494, 632, 745, 788, 1568
LweI	GCATC	7 cut(s)	1151, 1315, 1416, 1710, 1786, 2137, 2255
MabI	ACCWGGT	1 cut(s)	490
MaeI	CTAG	2 cut(s)	605, 1295
MaeII	ACGT	1 cut(s)	966
MaeIII	GTNAC	6 cut(s)	728, 976, 982, 1202, 1813, 2092
MflI	RGATCY	8 cut(s)	76, 233, 273, 607, 739, 1122, 1244, 2122
MhlI	GDGCHC	7 cut(s)	636, 790, 847, 1478, 1620, 1791, 1963
MlsI	TGGCCA	1 cut(s)	1281
MluNI	TGGCCA	1 cut(s)	1281
MlyI	GAGTC	2 cut(s)	1199, 1229
MmeI	TCCRAC	4 cut(s)	133, 271, 500, 1660
Mox20I	TGGCCA	1 cut(s)	1281
MroNI	GCCGGC	1 cut(s)	347
MroXI	GAANNNNTTC	2 cut(s)	127, 1991
MscI	TGGCCA	1 cut(s)	1281
MseI	TTAA	4 cut(s)	213, 324, 693, 1724
MslI	CAYNNNNRTG	5 cut(s)	1287, 1469, 1649, 2159, 2174
Msp20I	TGGCCA	1 cut(s)	1281
MspA1I	CMGCKG	2 cut(s)	368, 485
MspI	CCGG	2 cut(s)	60, 348
MspR9I	CCNGG	2 cut(s)	492, 1532
MvaI	CCWGG	2 cut(s)	492, 1532
MvnI	CGCG	1 cut(s)	368
NaeI	GCCGGC	1 cut(s)	349
NgoMIV	GCCGGC	1 cut(s)	347
NlaIII	CATG	8 cut(s)	26, 52, 221, 834, 961, 1510, 1946, 2173
NlaIV	GGNNCC	8 cut(s)	157, 275, 395, 424, 675, 707, 1908, 1994
NmeAIII	GCCGAG	1 cut(s)	2086
NmuCI	GTSAC	4 cut(s)	728, 982, 1202, 2092
NspI	RCATGY	1 cut(s)	2173
NspV	TTCGAA	1 cut(s)	1333
PceI	AGGCCT	1 cut(s)	603
PcsI	WCGNNNNNNNCGW	1 cut(s)	195
PdiI	GCCGGC	1 cut(s)	349
PdmI	GAANNNNTTC	2 cut(s)	127, 1991
PfeI	GAWTC	3 cut(s)	37, 1075, 1431
PinAI	ACCGGT	1 cut(s)	59
PkrI	GCNGC	7 cut(s)	34, 367, 484, 647, 760, 778, 1558
PleI	GAGTC	2 cut(s)	1199, 1228
PpsI	GAGTC	2 cut(s)	1199, 1228
PpuMI	RGGWCCY	2 cut(s)	674, 1855
Psp124BI	GAGCTC	4 cut(s)	790, 1620, 1791, 1963
Psp5II	RGGWCCY	2 cut(s)	674, 1855
Psp6I	CCWGG	2 cut(s)	490, 1530
PspFI	CCCAGC	1 cut(s)	419
PspGI	CCWGG	2 cut(s)	490, 1530
PspN4I	GGNNCC	8 cut(s)	157, 275, 395, 424, 675, 707, 1908, 1994
PspPI	GGNCC	5 cut(s)	423, 488, 623, 674, 1855
PspPPI	RGGWCCY	2 cut(s)	674, 1855
PstI	CTGCAG	2 cut(s)	484, 601
PstNI	CAGNNNCTG	1 cut(s)	1978
PsuI	RGATCY	8 cut(s)	76, 233, 273, 607, 739, 1122, 1244, 2122
PvuII	CAGCTG	1 cut(s)	485
RsaI	GTAC	8 cut(s)	64, 395, 804, 1055, 1195, 1398, 1941, 2311
RsaNI	GTAC	8 cut(s)	63, 394, 803, 1054, 1194, 1397, 1940, 2310
RseI	CAYNNNNRTG	5 cut(s)	1287, 1469, 1649, 2159, 2174
SacI	GAGCTC	4 cut(s)	790, 1620, 1791, 1963
SacII	CCGCGG	1 cut(s)	369
SaqAI	TTAA	4 cut(s)	213, 324, 693, 1724
SatI	GCNGC	7 cut(s)	33, 366, 483, 646, 759, 777, 1557
Sau96I	GGNCC	5 cut(s)	423, 488, 623, 674, 1855
SchI	GAGTC	2 cut(s)	1199, 1229
ScrFI	CCNGG	2 cut(s)	492, 1532
SduI	GDGCHC	7 cut(s)	636, 790, 847, 1478, 1620, 1791, 1963
SexAI	ACCWGGT	1 cut(s)	490
SfaNI	GCATC	7 cut(s)	1151, 1315, 1416, 1710, 1786, 2137, 2255
SfcI	CTRYAG	3 cut(s)	480, 597, 1779
Sfr303I	CCGCGG	1 cut(s)	369
SfuI	TTCGAA	1 cut(s)	1333
SgrBI	CCGCGG	1 cut(s)	369
SinI	GGWCC	3 cut(s)	488, 674, 1855
SmiMI	CAYNNNNRTG	5 cut(s)	1287, 1469, 1649, 2159, 2174
SmlI	CTYRAG	3 cut(s)	110, 1118, 1613
SmoI	CTYRAG	3 cut(s)	110, 1118, 1613
SseBI	AGGCCT	1 cut(s)	603
SsiI	CCGC	9 cut(s)	5, 32, 41, 366, 368, 441, 709, 733, 2262
SspI	AATATT	1 cut(s)	343
SspMI	CTAG	2 cut(s)	605, 1295
SstI	GAGCTC	4 cut(s)	790, 1620, 1791, 1963
StuI	AGGCCT	1 cut(s)	603
StyD4I	CCNGG	2 cut(s)	490, 1530
TaiI	ACGT	1 cut(s)	969
TatI	WGTACW	4 cut(s)	802, 1053, 1193, 1939
TauI	GCSGC	2 cut(s)	35, 368
TfiI	GAWTC	3 cut(s)	37, 1075, 1431
Tru1I	TTAA	4 cut(s)	213, 324, 693, 1724
Tru9I	TTAA	4 cut(s)	213, 324, 693, 1724
TscAI	CASTG	5 cut(s)	147, 979, 1144, 2163, 2181
TseFI	GTSAC	4 cut(s)	728, 982, 1202, 2092
TseI	GCWGC	5 cut(s)	482, 645, 758, 776, 1556
Tsp45I	GTSAC	4 cut(s)	728, 982, 1202, 2092
TspDTI	ATGAA	5 cut(s)	367, 397, 1254, 2118, 2256
TspGWI	ACGGA	3 cut(s)	80, 537, 1811
TspRI	CASTG	5 cut(s)	147, 979, 1144, 2163, 2181
VneI	GTGCAC	1 cut(s)	632
VpaK11BI	GGWCC	3 cut(s)	488, 674, 1855
XagI	CCTNNNNNAGG	2 cut(s)	818, 1970
XapI	RAATTY	1 cut(s)	2039
XceI	RCATGY	1 cut(s)	2173
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1468
XmiI	GTMKAC	1 cut(s)	496
XmnI	GAANNNNTTC	2 cut(s)	127, 1991
XspI	CTAG	2 cut(s)	605, 1295

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.