Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0005683

Physical Location & Seq

Genomic Coordinates Forward (+)

942385 .. 945779

Gene Structure 3395 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00077410.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2142 bp

ATGGAGGAACATTCAAACCATTATGCAGAAGCAGCAATTCCCCAGAACATGGAAGGAGAAGTTGAATCGGAAGAAAGAGATCTAGAAAGCAATTCACTGATCCAACCACTCCTCAAGAGAAACAGAACACTCTCTTCCAATCCACTGGCTCTGGTTGGAGCCAAAGTCTCCCACATCGAGAGTCTGGACTACGAGTACCTGATGGGGTTCATCTTTTTAGCTGTGACAAATCTTCTATTCACCGCAGTTGCCGCTGTTCTCTGCGTGTATTTCGCACCCACTGCAGCTGGACCTGGTATACCTGAAATCAAAGCTTATCTAAACGGAGTTGATACTCCCAATATGTTTGATAGTTGGAAGCATTGGGGCCGTCTCTGCAGGTCTAGATCTTGGAAAAGAAGGACCTCTGGTGCACATAGGAAGCTGCATTGCTTCCTTACTAGGCCAAGGGGGCCAGACAATTACCGTGTTAAATGGCGCTGGCTTCGCTACATGAATAACGACCGTGACCGCCGGGATCTCATTACCTGTGGGGCCGCCTCTGGGGTCTGTGCAGCTTTCCGATCCCCTGTGGGTGGTGTACTGTTTGCTCTTGAAGAGGTGGCAACATGGTGGAGGAGTGCCCTTCTGTGGAGAACTTTTTTCAGCACAGCCATTGTGGTGGTGGTGCTCAGGGCTTTTATTGAAATATGCAGCACCGGGGAATGTGGCCTTTTTGGAGAAGGAGGGCTTATCATGTTCGATGTCAGCACTGTAACTGTGACATACCGTTGGATGGATATTGTCCCTGTTGTTGTGATTGGCATTATTGGTGGCGTTTTGGGAAGCTTGTACAATTATTTTCTTCACAAGGTCCTCAGACTGTACAATCTCATAAATCAGAAGGGGAAAATTCACAAGATCCTCCTCAGTCTTGTTGTATCACTTTTCACCTCTGCGTGCCTATATGGTCTTCCTTTCCTTGCGAAATGTACACCCTGTGATCCCTCACTTACAGATTCTGTCTGCCCCACCAATGGACGGTCTGGCAACTTCAAACAGTTCAACTGCCCAGATGGCTACTATAATGACTTGGCTACTCTACTGCTTACCACTAATGATGATGCAGTTCGAAACATCTTCTCAACAAACACTTCCACTGAATATCACCCACTCAACCTCCTAATCTTCTTTGCACTCTTCTGCATCTTAGGATTGTTTACCTTTGGAATTGCTGTGCCGTCTGGCCTGTTCCTCCCCATCATCCTTATGGGTGCAGCCTATGGCCGCATGCTTGGTATTGCCATGAAATCGTACACAGACATTGACCAGGGACTCTACGCTGTTCTTGGTGCAGCTTCCCTAATGGCAGGATCGATGAGGATGACTGTTTCACTTTGTGTCATATTTCTTGAGCTGACCAACAATCTTCTCTTGCTGCCCATAACAATGATTGTCCTTCTAATTGCCAAAACTGTTGGAGACTGTTTCAACCCAAGCATCTATGAGATTATACTGCACTTAAAAGGTCTACCTTTCTTAGATGCACATCCAGAGCCATGGATGAGGAATCTGAATGTAGGTGAACTTGCAGATGCCAAGCCTCCAGTAGTCACCCTCCGTGGAATCGAAAAGGTGGAACGGATTGTGGAGGTCCTCAGAAACACCACACATAATGGCTTCCCTGTTGTAGATGATGCAGTTGTGCCCTCAGTAGGACTAGCTGTTGGGGCAACAGAACTACATGGACTAATACTAAGAGCTCACCTTCTCCAGGTGCTGAAGAAGAAGTGGTTCCTAAAGGAAAGGAGAAGAACAGAGGAGTGGGAAGTGAGAGAGAAGTTTACCGCAGTTGAATTAGCCGAGAGAGAAGGAAAAATTGAGGAGGTGGCTGTGACAAGTGATGAAATGGAGATGTATGTCGATCTGCATCCACTCACCAATACGACACCTCACACAGTGATTGAAAGCATGTCAGTGGCAAAAGCTATGGTGATTTTCAGACAAGTGGGACTCCGCCATTTGCTCATTCTGCCAAAGTACGAGGCAGCGGGGGTGCCTCCAGTGGTTGGGATCTTAACCAGGCAGGATTTGATAGCCTACAACATTTTGAATGCCTTTCCTCATCTCAAAAATCCCAAAGGCAGAGAGAAGGGGAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0001101 GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005247 GO:0005253 GO:0005254 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006812 GO:0006820 GO:0006821 GO:0008150 GO:0008308 GO:0008324 GO:0008509 GO:0009671 GO:0009705 GO:0010035 GO:0010167 GO:0015075 GO:0015077 GO:0015078 GO:0015103 GO:0015108 GO:0015112 GO:0015267 GO:0015291 GO:0015293 GO:0015294 GO:0015295 GO:0015296 GO:0015318 GO:0015513 GO:0015672 GO:0015698 GO:0015706 GO:0016020 GO:0022803 GO:0022804 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0022890 GO:0031090 GO:0034220 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0071705 GO:0098588 GO:0098655 GO:0098656 GO:0098660 GO:0098661 GO:0098662 GO:0098805 GO:1901698 GO:1901700 GO:1902476 GO:1902600

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBS Voltage_CLC

Protein Analysis

713

Amino Acids

79.54

Weight (kDa)

8.08

Isoelectric Point (pI)

40.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Voltage_CLC	PF00654	165 - 487	1.7e-55	Voltage gated chloride channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005683)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27170 AT5G40890 AT5G40890
fragaria_vesca	FvH4_6g53240 FvH4_6g53240 FvH4_6g53240 FvH4_6g53240 FvH4_6g53240 FvH4_6g53240
malus_domestica	MD17G1013000.v1.1
prunus_persica	Prupe.3G304600_v2.0.a1 Prupe.3G304600_v2.0.a1 Prupe.3G304600_v2.0.a1 Prupe.3G304600_v2.0.a1 Prupe.3G304600_v2.0.a1 Prupe.3G304600_v2.0.a1 Prupe.3G304600_v2.0.a1
pyrus_communis	pycom111g01040 pycom17g01040
rosa_laevigata	RLG00000022324
rosa_multiflora	Rmu_sc0003512.1_g000010
rosa_roxburghii	Rroxscaffold_2G00077410
rosa_rugosa	Rorug02G0583700
rosa_samantha	Rh2AG663800 Rh2BG674600 Rh2CG637800 Rh2DG688600
rosa_wichuraiana	Rw2G054400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	369
AccB1I	GGYRCC	1 cut(s)	2035
AccB7I	CCANNNNNTGG	2 cut(s)	49, 2048
AccI	GTMKAC	2 cut(s)	298, 1510
AciI	CCGC	8 cut(s)	243, 252, 511, 537, 1267, 1827, 1996, 2030
AclWI	GGATC	7 cut(s)	94, 525, 558, 895, 977, 1360, 2060
AcoI	YGGCCR	1 cut(s)	1264
AcsI	RAATTY	1 cut(s)	891
AcuI	CTGAAG	1 cut(s)	1781
AdeI	CACNNNGTG	3 cut(s)	980, 1379, 1939
AfaI	GTAC	7 cut(s)	197, 582, 833, 866, 973, 1295, 2021
AjnI	CCWGG	4 cut(s)	292, 1308, 1752, 2060
Alw21I	GWGCWC	3 cut(s)	415, 672, 1744
Alw26I	GTCTC	3 cut(s)	172, 377, 1455
Alw44I	GTGCAC	1 cut(s)	411
AlwI	GGATC	7 cut(s)	94, 525, 558, 895, 977, 1360, 2060
AlwNI	CAGNNNCTG	2 cut(s)	1001, 1759
AoxI	GGCC	7 cut(s)	367, 443, 452, 534, 709, 1225, 1264
ApaLI	GTGCAC	1 cut(s)	411
ApeKI	GCWGC	9 cut(s)	32, 284, 424, 554, 693, 1256, 1334, 1417, 2027
ApoI	RAATTY	1 cut(s)	891
Asp700I	GAANNNNTTC	1 cut(s)	1772
AspLEI	GCGC	1 cut(s)	480
AspS9I	GGNCC	7 cut(s)	290, 367, 402, 452, 534, 853, 1633
AsuC2I	CCSGG	2 cut(s)	515, 700
AsuHPI	GGTGA	8 cut(s)	232, 922, 1139, 1574, 1585, 1736, 1909, 1984
AsuII	TTCGAA	1 cut(s)	1111
AvaII	GGWCC	4 cut(s)	290, 402, 853, 1633
BaeGI	GKGCMC	3 cut(s)	415, 625, 1689
BanI	GGYRCC	1 cut(s)	2035
BanII	GRGCYC	1 cut(s)	1744
BbsI	GAAGAC	1 cut(s)	944
Bbv12I	GWGCWC	3 cut(s)	415, 672, 1744
BbvI	GCAGC	9 cut(s)	44, 296, 411, 566, 705, 1268, 1346, 1404, 2039
BccI	CCATC	4 cut(s)	196, 769, 1049, 1247
BceAI	ACGGC	2 cut(s)	354, 1204
BciT130I	CCWGG	4 cut(s)	294, 1310, 1754, 2062
BcnI	CCSGG	2 cut(s)	515, 700
BcoDI	GTCTC	3 cut(s)	172, 377, 1455
BfaI	CTAG	4 cut(s)	83, 384, 441, 1700
BfmI	CTRYAG	2 cut(s)	282, 376
BfoI	RGCGCY	1 cut(s)	481
BfuAI	ACCTGC	1 cut(s)	369
BglI	GCCNNNNNGGC	2 cut(s)	451, 1056
BglII	AGATCT	2 cut(s)	79, 386
Bme1390I	CCNGG	6 cut(s)	294, 515, 700, 1310, 1754, 2062
Bme18I	GGWCC	4 cut(s)	290, 402, 853, 1633
BmgT120I	GGNCC	7 cut(s)	290, 367, 402, 452, 534, 853, 1633
BmiI	GGNNCC	6 cut(s)	160, 368, 453, 535, 1775, 2037
BmrFI	CCNGG	6 cut(s)	294, 515, 700, 1310, 1754, 2062
BmsI	GCATC	7 cut(s)	1093, 1194, 1488, 1513, 1564, 1666, 1918
BpiI	GAAGAC	1 cut(s)	944
BpmI	CTGGAG	3 cut(s)	1569, 1736, 2025
Bpu10I	CCTNAGC	1 cut(s)	671
Bpu14I	TTCGAA	1 cut(s)	1111
BpuEI	CTTGAG	2 cut(s)	98, 1412
BpuMI	CCSGG	2 cut(s)	515, 700
Bsa29I	ATCGAT	1 cut(s)	1355
BsaBI	GATNNNNATC	2 cut(s)	1527, 1908
BsaJI	CCNNGG	5 cut(s)	446, 699, 1309, 1538, 1600
BsaXI	ACNNNNNCTCC	4 cut(s)	150, 180, 1143, 1173
Bse1I	ACTGG	3 cut(s)	150, 1586, 2042
Bse3DI	GCAATG	1 cut(s)	427
Bse8I	GATNNNNATC	2 cut(s)	1527, 1908
BseBI	CCWGG	4 cut(s)	294, 1310, 1754, 2062
BseCI	ATCGAT	1 cut(s)	1355
BseDI	CCNNGG	5 cut(s)	446, 699, 1309, 1538, 1600
BseGI	GGATG	6 cut(s)	780, 1242, 1368, 1528, 1548, 1909
BseJI	GATNNNNATC	2 cut(s)	1527, 1908
BseMI	GCAATG	1 cut(s)	427
BseMII	CTCAG	5 cut(s)	685, 871, 922, 1651, 1704
BseNI	ACTGG	3 cut(s)	150, 1586, 2042
BseRI	GAGGAG	5 cut(s)	101, 631, 896, 1814, 1877
BseSI	GKGCMC	3 cut(s)	415, 625, 1689
BseXI	GCAGC	9 cut(s)	44, 296, 411, 566, 705, 1268, 1346, 1404, 2039
BsgI	GTGCAG	4 cut(s)	573, 1275, 1353, 1481
Bsh1285I	CGRYCG	1 cut(s)	505
BshFI	GGCC	7 cut(s)	369, 445, 454, 536, 711, 1227, 1266
BshNI	GGYRCC	1 cut(s)	2035
BshVI	ATCGAT	1 cut(s)	1355
BsiEI	CGRYCG	1 cut(s)	505
BsiHKAI	GWGCWC	3 cut(s)	415, 672, 1744
BsiSI	CCGG	2 cut(s)	514, 699
BslFI	GGGAC	3 cut(s)	770, 1326, 2004
BsmAI	GTCTC	3 cut(s)	172, 377, 1455
BsmBI	CGTCTC	1 cut(s)	377
BsmFI	GGGAC	3 cut(s)	770, 1326, 2004
BsmI	GAATGC	1 cut(s)	2098
BsnI	GGCC	7 cut(s)	369, 445, 454, 536, 711, 1227, 1266
Bsp119I	TTCGAA	1 cut(s)	1111
Bsp1286I	GDGCHC	5 cut(s)	415, 625, 672, 1689, 1744
Bsp1407I	TGTACA	3 cut(s)	831, 864, 971
Bsp19I	CCATGG	1 cut(s)	1538
BspACI	CCGC	8 cut(s)	243, 252, 511, 537, 1267, 1827, 1996, 2030
BspANI	GGCC	7 cut(s)	369, 445, 454, 536, 711, 1227, 1266
BspCNI	CTCAG	5 cut(s)	684, 870, 921, 1650, 1703
BspDI	ATCGAT	1 cut(s)	1355
BspLI	GGNNCC	6 cut(s)	160, 368, 453, 535, 1775, 2037
BspMAI	CTGCAG	2 cut(s)	286, 380
BspMI	ACCTGC	1 cut(s)	369
BspPI	GGATC	7 cut(s)	94, 525, 558, 895, 977, 1360, 2060
BspT104I	TTCGAA	1 cut(s)	1111
BspT107I	GGYRCC	1 cut(s)	2035
BsrDI	GCAATG	1 cut(s)	427
BsrGI	TGTACA	3 cut(s)	831, 864, 971
BsrI	ACTGG	3 cut(s)	150, 1586, 2042
BssECI	CCNNGG	5 cut(s)	446, 699, 1309, 1538, 1600
BssNAI	GTATAC	1 cut(s)	299
BssT1I	CCWWGG	2 cut(s)	446, 1538
Bst1107I	GTATAC	1 cut(s)	299
Bst2UI	CCWGG	4 cut(s)	294, 1310, 1754, 2062
Bst6I	CTCTTC	3 cut(s)	139, 591, 1184
BstAPI	GCANNNNNTGC	1 cut(s)	281
BstAUI	TGTACA	3 cut(s)	831, 864, 971
BstBI	TTCGAA	1 cut(s)	1111
BstC8I	GCNNGC	3 cut(s)	482, 940, 1271
BstDEI	CTNAG	8 cut(s)	671, 857, 908, 1189, 1519, 1637, 1690, 1736
BstDSI	CCRYGG	2 cut(s)	1538, 1600
BstENI	CCTNNNNNAGG	1 cut(s)	1751
BstF5I	GGATG	6 cut(s)	780, 1242, 1368, 1528, 1548, 1909
BstH2I	RGCGCY	1 cut(s)	481
BstHHI	GCGC	1 cut(s)	480
BstMAI	GTCTC	3 cut(s)	172, 377, 1455
BstMCI	CGRYCG	1 cut(s)	505
BstMWI	GCNNNNNNNGC	9 cut(s)	32, 251, 281, 375, 451, 486, 1056, 1709, 2011
BstNI	CCWGG	4 cut(s)	294, 1310, 1754, 2062
BstNSI	RCATGY	2 cut(s)	1273, 1954
BstSCI	CCNGG	6 cut(s)	292, 513, 698, 1308, 1752, 2060
BstSFI	CTRYAG	2 cut(s)	282, 376
BstSLI	GKGCMC	3 cut(s)	415, 625, 1689
BstV1I	GCAGC	9 cut(s)	44, 296, 411, 566, 705, 1268, 1346, 1404, 2039
BstV2I	GAAGAC	1 cut(s)	944
BstX2I	RGATCY	5 cut(s)	79, 386, 517, 900, 2052
BstXI	CCANNNNNNTGG	3 cut(s)	145, 661, 1539
BstYI	RGATCY	5 cut(s)	79, 386, 517, 900, 2052
BstZ17I	GTATAC	1 cut(s)	299
Bsu15I	ATCGAT	1 cut(s)	1355
BsuRI	GGCC	7 cut(s)	369, 445, 454, 536, 711, 1227, 1266
BsuTUI	ATCGAT	1 cut(s)	1355
BtgI	CCRYGG	2 cut(s)	1538, 1600
BtsCI	GGATG	6 cut(s)	780, 1242, 1368, 1528, 1548, 1909
BtsI	GCAGTG	1 cut(s)	279
BtsIMutI	CAGTG	8 cut(s)	95, 143, 279, 750, 1137, 1944, 1962, 2049
BveI	ACCTGC	1 cut(s)	369
Cac8I	GCNNGC	3 cut(s)	482, 940, 1271
CaiI	CAGNNNCTG	2 cut(s)	1001, 1759
CfoI	GCGC	1 cut(s)	480
Cfr13I	GGNCC	7 cut(s)	290, 367, 402, 452, 534, 853, 1633
ClaI	ATCGAT	1 cut(s)	1355
CsiI	ACCWGGT	1 cut(s)	292
Csp6I	GTAC	7 cut(s)	196, 581, 832, 865, 972, 1294, 2020
CviAII	CATG	9 cut(s)	49, 493, 609, 736, 1270, 1285, 1539, 1724, 1951
CviQI	GTAC	7 cut(s)	196, 581, 832, 865, 972, 1294, 2020
DdeI	CTNAG	8 cut(s)	671, 857, 908, 1189, 1519, 1637, 1690, 1736
DraIII	CACNNNGTG	3 cut(s)	980, 1379, 1939
EaeI	YGGCCR	1 cut(s)	1264
Eam1104I	CTCTTC	3 cut(s)	139, 591, 1184
EarI	CTCTTC	3 cut(s)	139, 591, 1184
EciI	GGCGGA	1 cut(s)	1985
Ecl136II	GAGCTC	1 cut(s)	1742
Eco130I	CCWWGG	2 cut(s)	446, 1538
Eco24I	GRGCYC	1 cut(s)	1744
Eco47I	GGWCC	4 cut(s)	290, 402, 853, 1633
Eco53kI	GAGCTC	1 cut(s)	1742
Eco57I	CTGAAG	1 cut(s)	1781
EcoICRI	GAGCTC	1 cut(s)	1742
EcoNI	CCTNNNNNAGG	1 cut(s)	1751
EcoO109I	RGGNCCY	3 cut(s)	402, 853, 1633
EcoRII	CCWGG	4 cut(s)	292, 1308, 1752, 2060
EcoT14I	CCWWGG	2 cut(s)	446, 1538
EcoT38I	GRGCYC	1 cut(s)	1744
ErhI	CCWWGG	2 cut(s)	446, 1538
Esp3I	CGTCTC	1 cut(s)	377
FaeI	CATG	9 cut(s)	52, 496, 612, 739, 1273, 1288, 1542, 1727, 1954
FalI	AAGNNNNNCTT	2 cut(s)	714, 746
FaqI	GGGAC	3 cut(s)	770, 1326, 2004
FatI	CATG	9 cut(s)	48, 492, 608, 735, 1269, 1284, 1538, 1723, 1950
FauI	CCCGC	1 cut(s)	2023
FblI	GTMKAC	2 cut(s)	298, 1510
FokI	GGATG	6 cut(s)	787, 1229, 1375, 1515, 1555, 1896
FriOI	GRGCYC	1 cut(s)	1744
FspBI	CTAG	4 cut(s)	83, 384, 441, 1700
GlaI	GCGC	1 cut(s)	479
GsuI	CTGGAG	3 cut(s)	1569, 1736, 2025
HaeII	RGCGCY	1 cut(s)	481
HaeIII	GGCC	7 cut(s)	369, 445, 454, 536, 711, 1227, 1266
HapII	CCGG	2 cut(s)	514, 699
HhaI	GCGC	1 cut(s)	480
Hin1II	CATG	9 cut(s)	52, 496, 612, 739, 1273, 1288, 1542, 1727, 1954
Hin6I	GCGC	1 cut(s)	478
HinP1I	GCGC	1 cut(s)	478
HindIII	AAGCTT	2 cut(s)	312, 826
HinfI	GANTC	7 cut(s)	65, 181, 998, 1314, 1549, 1605, 1992
HpaII	CCGG	2 cut(s)	514, 699
HphI	GGTGA	8 cut(s)	232, 922, 1139, 1574, 1585, 1736, 1909, 1984
Hpy166II	GTNNAC	9 cut(s)	299, 413, 581, 974, 1200, 1296, 1511, 1565, 1824
Hpy188I	TCNGA	7 cut(s)	70, 563, 860, 882, 1554, 1640, 1982
Hpy188III	TCNNGA	8 cut(s)	83, 115, 178, 185, 384, 593, 1391, 1532
Hpy8I	GTNNAC	9 cut(s)	299, 413, 581, 974, 1200, 1296, 1511, 1565, 1824
HpyAV	CCTTC	9 cut(s)	47, 393, 635, 716, 877, 1448, 1757, 1844, 2125
HpyF10VI	GCNNNNNNNGC	9 cut(s)	32, 251, 281, 375, 451, 486, 1056, 1709, 2011
HpyF3I	CTNAG	8 cut(s)	671, 857, 908, 1189, 1519, 1637, 1690, 1736
Hsp92II	CATG	9 cut(s)	52, 496, 612, 739, 1273, 1288, 1542, 1727, 1954
HspAI	GCGC	1 cut(s)	478
LmnI	GCTCC	1 cut(s)	158
Lsp1109I	GCAGC	9 cut(s)	44, 296, 411, 566, 705, 1268, 1346, 1404, 2039
LweI	GCATC	7 cut(s)	1093, 1194, 1488, 1513, 1564, 1666, 1918
MabI	ACCWGGT	1 cut(s)	292
MaeI	CTAG	4 cut(s)	83, 384, 441, 1700
MaeIII	GTNAC	6 cut(s)	223, 506, 754, 760, 1591, 1873
MflI	RGATCY	5 cut(s)	79, 386, 517, 900, 2052
MhlI	GDGCHC	5 cut(s)	415, 625, 672, 1689, 1744
MluCI	AATT	9 cut(s)	36, 91, 460, 835, 891, 1209, 1443, 1835, 1857
MlyI	GAGTC	3 cut(s)	190, 1308, 1986
MmeI	TCCRAC	5 cut(s)	127, 136, 335, 752, 1438
MroXI	GAANNNNTTC	1 cut(s)	1772
MseI	TTAA	3 cut(s)	471, 1502, 2057
MslI	CAYNNNNRTG	4 cut(s)	659, 1247, 1427, 1955
MspA1I	CMGCKG	3 cut(s)	254, 287, 2030
MspI	CCGG	2 cut(s)	514, 699
MspR9I	CCNGG	6 cut(s)	294, 515, 700, 1310, 1754, 2062
Mva1269I	GAATGC	1 cut(s)	2098
MvaI	CCWGG	4 cut(s)	294, 1310, 1754, 2062
MwoI	GCNNNNNNNGC	9 cut(s)	32, 251, 281, 375, 451, 486, 1056, 1709, 2011
NciI	CCSGG	2 cut(s)	515, 700
NcoI	CCATGG	1 cut(s)	1538
NlaIII	CATG	9 cut(s)	52, 496, 612, 739, 1273, 1288, 1542, 1727, 1954
NlaIV	GGNNCC	6 cut(s)	160, 368, 453, 535, 1775, 2037
NmeAIII	GCCGAG	1 cut(s)	1867
NmuCI	GTSAC	5 cut(s)	223, 506, 760, 1591, 1873
NspI	RCATGY	2 cut(s)	1273, 1954
NspV	TTCGAA	1 cut(s)	1111
PaeI	GCATGC	1 cut(s)	1273
PctI	GAATGC	1 cut(s)	2098
PdmI	GAANNNNTTC	1 cut(s)	1772
PfeI	GAWTC	4 cut(s)	65, 998, 1549, 1605
PflMI	CCANNNNNTGG	2 cut(s)	49, 2048
PleI	GAGTC	3 cut(s)	189, 1308, 1986
PpsI	GAGTC	3 cut(s)	189, 1308, 1986
PpuMI	RGGWCCY	3 cut(s)	402, 853, 1633
Psp124BI	GAGCTC	1 cut(s)	1744
Psp5II	RGGWCCY	3 cut(s)	402, 853, 1633
Psp6I	CCWGG	4 cut(s)	292, 1308, 1752, 2060
PspGI	CCWGG	4 cut(s)	292, 1308, 1752, 2060
PspN4I	GGNNCC	6 cut(s)	160, 368, 453, 535, 1775, 2037
PspPI	GGNCC	7 cut(s)	290, 367, 402, 452, 534, 853, 1633
PspPPI	RGGWCCY	3 cut(s)	402, 853, 1633
PstI	CTGCAG	2 cut(s)	286, 380
PstNI	CAGNNNCTG	2 cut(s)	1001, 1759
PsuI	RGATCY	5 cut(s)	79, 386, 517, 900, 2052
PvuII	CAGCTG	1 cut(s)	287
RsaI	GTAC	7 cut(s)	197, 582, 833, 866, 973, 1295, 2021
RsaNI	GTAC	7 cut(s)	196, 581, 832, 865, 972, 1294, 2020
RseI	CAYNNNNRTG	4 cut(s)	659, 1247, 1427, 1955
SacI	GAGCTC	1 cut(s)	1744
SaqAI	TTAA	3 cut(s)	471, 1502, 2057
Sau96I	GGNCC	7 cut(s)	290, 367, 402, 452, 534, 853, 1633
SchI	GAGTC	3 cut(s)	190, 1308, 1986
ScrFI	CCNGG	6 cut(s)	294, 515, 700, 1310, 1754, 2062
SduI	GDGCHC	5 cut(s)	415, 625, 672, 1689, 1744
SexAI	ACCWGGT	1 cut(s)	292
SfaNI	GCATC	7 cut(s)	1093, 1194, 1488, 1513, 1564, 1666, 1918
SfcI	CTRYAG	2 cut(s)	282, 376
SfiI	GGCCNNNNNGGCC	1 cut(s)	451
SfuI	TTCGAA	1 cut(s)	1111
SinI	GGWCC	4 cut(s)	290, 402, 853, 1633
SmiMI	CAYNNNNRTG	4 cut(s)	659, 1247, 1427, 1955
SmlI	CTYRAG	2 cut(s)	113, 1391
SmoI	CTYRAG	2 cut(s)	113, 1391
SphI	GCATGC	1 cut(s)	1273
Sse9I	AATT	9 cut(s)	36, 91, 460, 835, 891, 1209, 1443, 1835, 1857
SsiI	CCGC	8 cut(s)	243, 252, 511, 537, 1267, 1827, 1996, 2030
SspMI	CTAG	4 cut(s)	83, 384, 441, 1700
SstI	GAGCTC	1 cut(s)	1744
StyD4I	CCNGG	6 cut(s)	292, 513, 698, 1308, 1752, 2060
StyI	CCWWGG	2 cut(s)	446, 1538
TaqI	TCGA	6 cut(s)	177, 741, 1111, 1355, 1608, 1902
TasI	AATT	9 cut(s)	36, 91, 460, 835, 891, 1209, 1443, 1835, 1857
TatI	WGTACW	4 cut(s)	580, 831, 864, 971
TauI	GCSGC	3 cut(s)	254, 539, 1269
TfiI	GAWTC	4 cut(s)	65, 998, 1549, 1605
Tru1I	TTAA	3 cut(s)	471, 1502, 2057
Tru9I	TTAA	3 cut(s)	471, 1502, 2057
TscAI	CASTG	8 cut(s)	102, 150, 286, 757, 1144, 1944, 1962, 2049
TseFI	GTSAC	5 cut(s)	223, 506, 760, 1591, 1873
TseI	GCWGC	9 cut(s)	32, 284, 424, 554, 693, 1256, 1334, 1417, 2027
Tsp45I	GTSAC	5 cut(s)	223, 506, 760, 1591, 1873
TspDTI	ATGAA	4 cut(s)	199, 509, 1301, 1899
TspGWI	ACGGA	3 cut(s)	339, 1589, 1636
TspRI	CASTG	8 cut(s)	102, 150, 286, 757, 1144, 1944, 1962, 2049
Van91I	CCANNNNNTGG	2 cut(s)	49, 2048
VneI	GTGCAC	1 cut(s)	411
VpaK11BI	GGWCC	4 cut(s)	290, 402, 853, 1633
XagI	CCTNNNNNAGG	1 cut(s)	1751
XapI	RAATTY	1 cut(s)	891
XbaI	TCTAGA	2 cut(s)	82, 383
XceI	RCATGY	2 cut(s)	1273, 1954
XcmI	CCANNNNNNNNNTGG	2 cut(s)	661, 1246
XmiI	GTMKAC	2 cut(s)	298, 1510
XmnI	GAANNNNTTC	1 cut(s)	1772
XspI	CTAG	4 cut(s)	83, 384, 441, 1700

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.