MD17G1146000.v1.1

Lateral organ boundaries (LOB) domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0024297
Physical Location & Seq
Forward (+)
13182274 .. 13183569
1296 bp
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UTR
Exon/CDS
Intron
MD17G1146000.v1.1.491

Sequence Viewer

Length: 471 bp
ATGTCTAATCCAACGAGATGTGCAGCTTGCAGATTTTTGCGAAGAAGATGCCCTCAAGATTGTGTTTTGGCACCCTATTTTCCCTCCTCCAATCCTGAGAGATTTTCTTGTGTCCATAGGATCTTTGGTGCCAGCAACATTACCACAATGCTAGAGCAACTTCCAGTCCATTTAAGAGAAGCAGCAGCAGACTCCATGTTCTACGAGGCATCGTTACGTGTTGAAGATCCGATTTACGGATGTGTTAGAATCATTTCTCAGCTGCAACACCATATATTGGAGGCTCAATCTGAGTTACTCAAGACCAACGGTCAAATAGCATCCAACCTTGCACAACACCAACTGCACCAGCAGCAGCAGCAAAATGGTGTTCATGGTGTTAGTTACCACCAAGACCAGCAGATCAGTGAACCTTCGGGGCTCAGCTTGGACATGCAAGCTTTCGCAGATGACTGTTACCTTCACCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

157

Amino Acids

17.65

Weight (kDa)

6.1

Isoelectric Point (pI)

63.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 6 - 102 3.7e-38 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024297)

Species Orthologous Gene IDs
malus_domestica MD09G1160200.v1.1 MD17G1146000.v1.1
prunus_persica Prupe.3G018800_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 70, 128
AccB7I CCANNNNNTGG 1 cut(s) 277
AclWI GGATC 2 cut(s) 128, 221
AfiI CCNNNNNNNGG 2 cut(s) 236, 277
AflIII ACRYGT 1 cut(s) 217
AgsI TTSAA 1 cut(s) 224
AhdI GACNNNNNGTC 1 cut(s) 309
AluBI AGCT 4 cut(s) 26, 262, 426, 440
AluI AGCT 4 cut(s) 26, 262, 426, 440
AlwI GGATC 2 cut(s) 128, 221
ApeKI GCWGC 7 cut(s) 23, 182, 185, 262, 352, 355, 358
Asp700I GAANNNNTTC 1 cut(s) 253
AsuHPI GGTGA 1 cut(s) 455
BanI GGYRCC 2 cut(s) 70, 128
BanII GRGCYC 1 cut(s) 423
BbvI GCAGC 7 cut(s) 35, 194, 197, 249, 364, 367, 370
BcgI CGANNNNNNTGC 2 cut(s) 30, 64
BfaI CTAG 1 cut(s) 152
BisI GCNGC 7 cut(s) 24, 183, 186, 263, 353, 356, 359
BlpI GCTNAGC 1 cut(s) 422
BlsI GCNGC 7 cut(s) 25, 184, 187, 264, 354, 357, 360
BmeRI GACNNNNNGTC 1 cut(s) 309
BmiI GGNNCC 2 cut(s) 72, 130
BmsI GCATC 3 cut(s) 38, 218, 329
Bpu1102I GCTNAGC 1 cut(s) 422
BpuEI CTTGAG 2 cut(s) 39, 284
BsaAI YACGTR 1 cut(s) 218
Bsc4I CCNNNNNNNGG 2 cut(s) 236, 277
Bse1I ACTGG 1 cut(s) 164
BseGI GGATG 2 cut(s) 245, 320
BseLI CCNNNNNNNGG 2 cut(s) 236, 277
BseMII CTCAG 4 cut(s) 87, 272, 282, 436
BseNI ACTGG 1 cut(s) 164
BseRI GAGGAG 1 cut(s) 76
BseXI GCAGC 7 cut(s) 35, 194, 197, 249, 364, 367, 370
BsgI GTGCAG 2 cut(s) 42, 329
BshNI GGYRCC 2 cut(s) 70, 128
BslI CCNNNNNNNGG 2 cut(s) 236, 277
Bsp1286I GDGCHC 1 cut(s) 423
Bsp143I GATC 3 cut(s) 120, 226, 402
Bsp1720I GCTNAGC 1 cut(s) 422
BspCNI CTCAG 4 cut(s) 88, 271, 283, 435
BspLI GGNNCC 2 cut(s) 72, 130
BspPI GGATC 2 cut(s) 128, 221
BspT107I GGYRCC 2 cut(s) 70, 128
BsrI ACTGG 1 cut(s) 164
BssMI GATC 3 cut(s) 120, 226, 402
Bst4CI ACNGT 2 cut(s) 311, 455
BstBAI YACGTR 1 cut(s) 218
BstC8I GCNNGC 3 cut(s) 28, 133, 438
BstDEI CTNAG 4 cut(s) 96, 258, 291, 422
BstF5I GGATG 2 cut(s) 245, 320
BstKTI GATC 3 cut(s) 123, 229, 405
BstMBI GATC 3 cut(s) 120, 226, 402
BstMWI GCNNNNNNNGC 2 cut(s) 352, 358
BstNSI RCATGY 1 cut(s) 436
BstV1I GCAGC 7 cut(s) 35, 194, 197, 249, 364, 367, 370
BstX2I RGATCY 2 cut(s) 120, 226
BstYI RGATCY 2 cut(s) 120, 226
BtsCI GGATG 2 cut(s) 245, 320
BtsIMutI CAGTG 1 cut(s) 412
Cac8I GCNNGC 3 cut(s) 28, 133, 438
CviAII CATG 3 cut(s) 196, 374, 433
CviJI RGCY 6 cut(s) 26, 262, 284, 421, 426, 440
CviKI_1 RGCY 6 cut(s) 26, 262, 284, 421, 426, 440
DdeI CTNAG 4 cut(s) 96, 258, 291, 422
DpnI GATC 3 cut(s) 122, 228, 404
DpnII GATC 3 cut(s) 120, 226, 402
DriI GACNNNNNGTC 1 cut(s) 309
Eam1105I GACNNNNNGTC 1 cut(s) 309
Eco24I GRGCYC 1 cut(s) 423
EcoT38I GRGCYC 1 cut(s) 423
FaeI CATG 3 cut(s) 199, 377, 436
FaiI YATR 6 cut(s) 117, 197, 273, 275, 375, 434
FatI CATG 3 cut(s) 195, 373, 432
Fnu4HI GCNGC 7 cut(s) 24, 183, 186, 263, 353, 356, 359
FokI GGATG 2 cut(s) 252, 307
FriOI GRGCYC 1 cut(s) 423
Fsp4HI GCNGC 7 cut(s) 24, 183, 186, 263, 353, 356, 359
FspBI CTAG 1 cut(s) 152
GluI GCNGC 7 cut(s) 24, 183, 186, 263, 353, 356, 359
Hin1II CATG 3 cut(s) 199, 377, 436
HindIII AAGCTT 1 cut(s) 438
HinfI GANTC 2 cut(s) 191, 249
HphI GGTGA 1 cut(s) 455
Hpy166II GTNNAC 1 cut(s) 410
Hpy188I TCNGA 2 cut(s) 231, 292
Hpy188III TCNNGA 3 cut(s) 56, 95, 301
Hpy8I GTNNAC 1 cut(s) 410
HpyAV CCTTC 2 cut(s) 423, 470
HpyCH4III ACNGT 2 cut(s) 311, 455
HpyCH4IV ACGT 1 cut(s) 217
HpyCH4V TGCA 6 cut(s) 23, 30, 265, 332, 346, 436
HpyF10VI GCNNNNNNNGC 2 cut(s) 352, 358
HpyF3I CTNAG 4 cut(s) 96, 258, 291, 422
HpySE526I ACGT 1 cut(s) 217
Hsp92II CATG 3 cut(s) 199, 377, 436
Kzo9I GATC 3 cut(s) 120, 226, 402
LpnPI CCDG 5 cut(s) 108, 145, 177, 362, 410
Lsp1109I GCAGC 7 cut(s) 35, 194, 197, 249, 364, 367, 370
LweI GCATC 3 cut(s) 38, 218, 329
MaeI CTAG 1 cut(s) 152
MaeII ACGT 1 cut(s) 217
MaeIII GTNAC 4 cut(s) 213, 294, 383, 455
MalI GATC 3 cut(s) 122, 228, 404
MboI GATC 3 cut(s) 120, 226, 402
MboII GAAGA 3 cut(s) 54, 57, 236
MflI RGATCY 2 cut(s) 120, 226
MhlI GDGCHC 1 cut(s) 423
MlyI GAGTC 1 cut(s) 185
MmeI TCCRAC 2 cut(s) 35, 348
MnlI CCTC 5 cut(s) 63, 94, 97, 199, 274
MroXI GAANNNNTTC 1 cut(s) 253
MseI TTAA 1 cut(s) 173
MspA1I CMGCKG 1 cut(s) 262
MwoI GCNNNNNNNGC 2 cut(s) 352, 358
NdeII GATC 3 cut(s) 120, 226, 402
NlaIII CATG 3 cut(s) 199, 377, 436
NlaIV GGNNCC 2 cut(s) 72, 130
NspI RCATGY 1 cut(s) 436
PdmI GAANNNNTTC 1 cut(s) 253
PfeI GAWTC 1 cut(s) 249
PflMI CCANNNNNTGG 1 cut(s) 277
PkrI GCNGC 7 cut(s) 25, 184, 187, 264, 354, 357, 360
PleI GAGTC 1 cut(s) 185
PpsI GAGTC 1 cut(s) 185
Ppu21I YACGTR 1 cut(s) 218
PspN4I GGNNCC 2 cut(s) 72, 130
PsuI RGATCY 2 cut(s) 120, 226
PvuII CAGCTG 1 cut(s) 262
SaqAI TTAA 1 cut(s) 173
SatI GCNGC 7 cut(s) 24, 183, 186, 263, 353, 356, 359
Sau3AI GATC 3 cut(s) 120, 226, 402
SchI GAGTC 1 cut(s) 185
SduI GDGCHC 1 cut(s) 423
SetI ASST 8 cut(s) 28, 220, 264, 330, 415, 428, 442, 462
SfaNI GCATC 3 cut(s) 38, 218, 329
SmlI CTYRAG 2 cut(s) 54, 299
SmoI CTYRAG 2 cut(s) 54, 299
SspMI CTAG 1 cut(s) 152
TaaI ACNGT 2 cut(s) 311, 455
TaiI ACGT 1 cut(s) 220
TfiI GAWTC 1 cut(s) 249
Tru1I TTAA 1 cut(s) 173
Tru9I TTAA 1 cut(s) 173
TscAI CASTG 1 cut(s) 412
TseI GCWGC 7 cut(s) 23, 182, 185, 262, 352, 355, 358
TspDTI ATGAA 1 cut(s) 362
TspGWI ACGGA 1 cut(s) 252
TspRI CASTG 1 cut(s) 412
Van91I CCANNNNNTGG 1 cut(s) 277
XceI RCATGY 1 cut(s) 436
XcmI CCANNNNNNNNNTGG 1 cut(s) 122
XmnI GAANNNNTTC 1 cut(s) 253
XspI CTAG 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.