MD17G1217600.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0024441
Physical Location & Seq
Reverse (-)
26440744 .. 26443464
2721 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1217600.v1.1.491

Sequence Viewer

Length: 2721 bp
ATGATACATCGTCATTTATCTCACTTGCGTTCGCTCTCCACTCTCTCTTCCCCAACTTCACAAATCCGTCATCTCTTGCAATGCAACAAAGAAATCCACAGGCTCTCCCAACTCGGCCGATTAACAGATGCACGCCAGGTGTTTGAAAGAATGCCTCAACGAGACTCGGGCTCTTGGAACACTATGATTTCTGCGTACATCCAAAACGGGCATTTGAACAAAGCCCAAGAACTCTTTGATTCATTCCGAGATAAAAATGTGAGAACTTGGACCATTTTGCTATCCGGGTATGCGAAACATGGGCATGCGGGTGAAGCTCAAGCGGTGTTTGAGTCAATGCCTGAGCGTAATGTAGTTTCGTGGAATGCTATGATTACTGCTTATACACAAAATGGTTTGTTAAATAGTGCTAGGGACGTTTTTGATCAAATGCCTGAGAGAAATATAGTGTCATGGAATTCAATTATTACCGGGTACTGTCGTTGCGGTAGGATTAATGTAGCTCGTGAGCTTTTCGATCAAATGGAGGACAGGAACATTGCCTCTTGGATGGTTATGGTTTCTGGTTATGTTGAGATAGGTGAGTTTCATGAGGCTTTGATGGTTTTTTTGATGATGTTGAGGAGCAGTGTGAGGCCAGACCAGGCCATACTAGTTGCTGCCTTATCAGCTGTGATGAGGTTAGAAAAATTGGAGTTGATTGAGAGTTTAAGGGTACTGGCTTTGAAGACAGGGTGTGAGAGCAATGTGGTAGTGGACACGGCAATATTGAATGCTTATACAGCAAATGGAAGTTTGGAAGATGCGGTGAAGTTTTTCATGAGAATGCCACAAAGGAATGATTACTCATGTTCGACAATGATTGCTGCATTTTCGCGGTGTGGTAGGTTGGATGATGCCATTGCATTGTATGAGATAGACGCTGAAAAAGGTGTCGGTACACAGACGGCGATGGTAACTGCCTATGCTCAGAATGGGAGGATTCACAAAGCAAGGCATATATTTGATAAAATTGGAAACCCTACTGTGGTTGCATGGAATGCTATGGTTGCAGGGTATGCCCAGAACAGAATGCTTGAAGAAGCAAAAGATATTTTTTCCAAAATCCCGGTACCAAATGCTGCTTCATGGGCAGCCCTGATTTCTGGGTTTGTCCAGAACGGACAAAGCAAAGAAGCTCTGGAGCTCTTTGCTGAGCTTCATAGATCAGGCACTGTACTGCCAAGTCATTCAAATTTCACCAGTGCTCTCTTTGCATGTGCAAATATCAACAATGTTGAGATGGGAAAACAAATACACTCGCTTGCTATTAAAACAAGGTACCAATTCAATTCATTCGTGGGAAATGGGTTGATATCAATGTATGCAAAGTGCAAAACCACATATATATCACAAGCTTCTAGGATAAAGAGAGTGAGAGACACCGTATCTTGGGTTTCAGAAAGTTGTACGTTGGATGATGCTCGAAAAGCTTTTGAACAAATGCCAAAACGGGATGTTGTGTCATGGTCTGCTATTATATCAGCTTATGAGCAAGCCGGGCAAGGAGATATTGCGTTCAAATTGTTCCTTGAAATGTTATCCAGGGGACTGAAACCAAACCAATCAACAATAACCAGCCTCCTCAGTGCTTGCGGGTACCTTGGTGCAACCAAGCTTGGGGAACAGATTCATGCCTTGATACACAAACTTGGACTGGATTCATGTTTGTTTGTGTGCAATGCTTTGATAACAATGTACTTCAAGTGTGGAAGCCCCAATGGCCTTTGTATCTTTCAAGAGATGCCTGATCGAGATATTGTCACTTGGAATGCTGTTTTGACCGGGTGTGCTCAAAACGGATCAGGCAAAGAAGCTGTTGAAGTTTTCAAACAAATGGAAGCTACTGGGATTTCTCCGAATGAAATTAGTTTTGTAGCACTGTTATGCGCTTGTAGCCATGCTGGGTTAGTAGACGAAGGATGGGCTTATTTCAATTCCATGAGCCAACATTATGGGATTATGCCCTTGGTTTATCACTATACTTGTATGGTTGATCTCCTGGGTAGGGCAGGATTGCTTTCTGAATCTGAAGATCTCATTCAGAGCATGCCGGTAGAACCAGATTCCGTGATTTGGGAAGCACTTCTTGGTGCCTGTAGGATCCATCGAAACACTGAACTTGGCCAGAGAACGGCTGAAAGGCTTTTCCAAATGGGAACACGGAGATCTGGAACCTATGTTTTATTATCAAATATGTATGCATCTAAAGGCATGTGGGAGAAAGTGCGGGAAGTAAGGGAAATGATGAAAGATAGGGGAGTGACCAAAGAACCCGGATTCAGTTGGATTCAGATCAAGAATAAGGTTCACTATTTTCTAATGGGGGATAAGGCACATGACAACATCAAAGAGATAAACCTGACAGTAAATGAGTTGTACAAACGCTTCCGAGCAACAGGTTATGTGCCGGATACTAGTTTTGTTCTTCATGATGTGGCAGAGGAGCAAAAAGAGGATGACCTTGTATACCACAGTGAGAAACTTGCTGTTGCATATGGAATCTTGCAGACACCAAATGGTAGTCCAATTCAGATTCTGAAGAACCTCCGAATATGCGGTGACTGCCATTCGTTTATGAAATTTGTCTCCAGTGTTGCCAAGCGCAAAATCATTCTCCGAGATGGAAATAGGTTCCACCATTTTGAAGATGGTTTGTGCTCTTGTGGTGATTATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR

Protein Analysis

907

Amino Acids

101.71

Weight (kDa)

7.75

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 58 - 87 3.5e-06 PPR repeat
PPR PF01535 89 - 118 5.7e-06 PPR repeat
PPR_2 PF13041 116 - 149 2.2e-07 PPR repeat family
PPR PF01535 119 - 149 1.9e-07 PPR repeat
PPR_2 PF13041 147 - 180 1.3e-09 PPR repeat family
PPR_1 PF12854 147 - 176 1.5e-09 PPR repeat
PPR PF01535 150 - 180 3.4e-08 PPR repeat
PPR_2 PF13041 498 - 545 2.9e-12 PPR repeat family
PPR PF01535 501 - 531 5.3e-06 PPR repeat
PPR_2 PF13041 598 - 646 8e-12 PPR repeat family
PPR_3 PF13812 599 - 645 9e-09 Pentatricopeptide repeat domain
PPR PF01535 601 - 630 8.4e-07 PPR repeat
E_motif PF20431 716 - 778 2.4e-17 E motif
DYW_deaminase PF14432 814 - 906 5.5e-44 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024441)

Species Orthologous Gene IDs
malus_domestica MD17G1217600.v1.1
prunus_persica Prupe.3G093300_v2.0.a1
pyrus_communis pycom17g22190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 3 cut(s) 1111, 1320, 1638
AccB1I GGYRCC 4 cut(s) 1111, 1320, 1638, 2132
AccI GTMKAC 2 cut(s) 1953, 2508
AccII CGCG 1 cut(s) 877
AciI CCGC 8 cut(s) 308, 323, 486, 806, 877, 1635, 2269, 2598
AclWI GGATC 3 cut(s) 1849, 2137, 2150
AcoI YGGCCR 2 cut(s) 115, 2164
AcsI RAATTY 3 cut(s) 457, 1234, 2621
AcuI CTGAAG 2 cut(s) 2091, 2600
AfiI CCNNNNNNNGG 6 cut(s) 1027, 1431, 1659, 2047, 2115, 2173
AhlI ACTAGT 2 cut(s) 652, 2456
AjnI CCWGG 4 cut(s) 135, 642, 1583, 2040
AjuI GAANNNNNNNTTGG 4 cut(s) 1108, 1140, 2112, 2144
Alw21I GWGCWC 4 cut(s) 1188, 1249, 1834, 2702
Alw26I GTCTC 3 cut(s) 156, 1413, 2632
AlwI GGATC 3 cut(s) 1849, 2137, 2150
AlwNI CAGNNNCTG 2 cut(s) 1214, 2578
Ama87I CYCGRG 1 cut(s) 166
AoxI GGCC 5 cut(s) 115, 635, 645, 1762, 2164
ApeKI GCWGC 4 cut(s) 659, 866, 1121, 1133
ApoI RAATTY 3 cut(s) 457, 1234, 2621
AseI ATTAAT 1 cut(s) 495
Asp700I GAANNNNTTC 3 cut(s) 815, 1668, 2124
Asp718I GGTACC 3 cut(s) 1111, 1320, 1638
AspLEI GCGC 2 cut(s) 1931, 2646
AspS9I GGNCC 1 cut(s) 270
AsuC2I CCSGG 6 cut(s) 286, 472, 1109, 1540, 1825, 2316
AsuHPI GGTGA 6 cut(s) 323, 593, 820, 1231, 2612, 2720
AvaI CYCGRG 1 cut(s) 166
AvaII GGWCC 1 cut(s) 270
BaeI ACNNNNGTAYC 4 cut(s) 466, 499, 1630, 1663
BalI TGGCCA 1 cut(s) 2166
BamHI GGATCC 1 cut(s) 2142
BanI GGYRCC 4 cut(s) 1111, 1320, 1638, 2132
BanII GRGCYC 2 cut(s) 173, 1188
BarI GAAGNNNNNNTAC 2 cut(s) 2411, 2443
BauI CACGAG 1 cut(s) 504
BbsI GAAGAC 1 cut(s) 734
Bbv12I GWGCWC 4 cut(s) 1188, 1249, 1834, 2702
BbvI GCAGC 4 cut(s) 646, 853, 1108, 1145
BccI CCATC 8 cut(s) 544, 595, 946, 1276, 1956, 2154, 2657, 2684
BceAI ACGGC 3 cut(s) 777, 963, 2190
BciT130I CCWGG 4 cut(s) 137, 644, 1585, 2042
BciVI GTATCC 1 cut(s) 2446
BclI TGATCA 1 cut(s) 424
BcnI CCSGG 6 cut(s) 286, 472, 1109, 1540, 1825, 2316
BcoDI GTCTC 3 cut(s) 156, 1413, 2632
BcuI ACTAGT 2 cut(s) 652, 2456
BfaI CTAG 4 cut(s) 411, 653, 1401, 2457
BfmI CTRYAG 1 cut(s) 2137
BfuI GTATCC 1 cut(s) 2446
BglI GCCNNNNNGGC 1 cut(s) 1761
BglII AGATCT 2 cut(s) 2074, 2207
BisI GCNGC 4 cut(s) 660, 867, 1122, 1134
BlpI GCTNAGC 1 cut(s) 1194
BlsI GCNGC 4 cut(s) 661, 868, 1123, 1135
Bme18I GGWCC 1 cut(s) 270
BmeT110I CYCGRG 1 cut(s) 166
BmgT120I GGNCC 1 cut(s) 270
BmiI GGNNCC 7 cut(s) 1113, 1322, 1640, 2134, 2144, 2215, 2675
BmrI ACTGGG 1 cut(s) 1896
BmsI GCATC 6 cut(s) 118, 793, 886, 1450, 1773, 2252
BmuI ACTGGG 1 cut(s) 1896
BpiI GAAGAC 1 cut(s) 734
BpmI CTGGAG 2 cut(s) 1202, 2614
Bpu10I CCTNAGC 1 cut(s) 342
Bpu1102I GCTNAGC 1 cut(s) 1194
BpuEI CTTGAG 1 cut(s) 303
BpuMI CCSGG 6 cut(s) 286, 472, 1109, 1540, 1825, 2316
BsaBI GATNNNNATC 1 cut(s) 2333
BsaJI CCNNGG 4 cut(s) 1584, 1642, 2007, 2041
BsaXI ACNNNNNCTCC 2 cut(s) 89, 119
Bsc4I CCNNNNNNNGG 6 cut(s) 1027, 1431, 1659, 2047, 2115, 2173
Bse118I RCCGGY 1 cut(s) 2092
Bse1I ACTGG 5 cut(s) 723, 1242, 1701, 1891, 2631
Bse3DI GCAATG 5 cut(s) 86, 537, 751, 900, 1726
Bse8I GATNNNNATC 1 cut(s) 2333
BseBI CCWGG 4 cut(s) 137, 644, 1585, 2042
BseDI CCNNGG 4 cut(s) 1584, 1642, 2007, 2041
BseGI GGATG 7 cut(s) 198, 555, 898, 1462, 1501, 1967, 2503
BseJI GATNNNNATC 1 cut(s) 2333
BseLI CCNNNNNNNGG 6 cut(s) 1027, 1431, 1659, 2047, 2115, 2173
BseMI GCAATG 5 cut(s) 86, 537, 751, 900, 1726
BseMII CTCAG 5 cut(s) 333, 426, 983, 1185, 1639
BseNI ACTGG 5 cut(s) 723, 1242, 1701, 1891, 2631
BseRI GAGGAG 3 cut(s) 637, 1613, 2498
BseX3I CGGCCG 1 cut(s) 115
BseXI GCAGC 4 cut(s) 646, 853, 1108, 1145
BseYI CCCAGC 1 cut(s) 1943
Bsh1236I CGCG 1 cut(s) 877
Bsh1285I CGRYCG 1 cut(s) 118
BshFI GGCC 5 cut(s) 117, 637, 647, 1764, 2166
BshNI GGYRCC 4 cut(s) 1111, 1320, 1638, 2132
BsiEI CGRYCG 1 cut(s) 118
BsiHKAI GWGCWC 4 cut(s) 1188, 1249, 1834, 2702
BsiHKCI CYCGRG 1 cut(s) 166
BsiSI CCGG 8 cut(s) 285, 471, 1109, 1539, 1824, 2093, 2316, 2450
BslFI GGGAC 2 cut(s) 428, 1602
BslI CCNNNNNNNGG 6 cut(s) 1027, 1431, 1659, 2047, 2115, 2173
BsmAI GTCTC 3 cut(s) 156, 1413, 2632
BsmFI GGGAC 2 cut(s) 428, 1602
BsmI GAATGC 7 cut(s) 156, 370, 778, 831, 1045, 1077, 1816
BsnI GGCC 5 cut(s) 117, 637, 647, 1764, 2166
BsoBI CYCGRG 1 cut(s) 166
Bsp1286I GDGCHC 5 cut(s) 173, 1188, 1249, 1834, 2702
Bsp1407I TGTACA 1 cut(s) 2418
Bsp1720I GCTNAGC 1 cut(s) 1194
BspACI CCGC 8 cut(s) 308, 323, 486, 806, 877, 1635, 2269, 2598
BspANI GGCC 5 cut(s) 117, 637, 647, 1764, 2166
BspCNI CTCAG 5 cut(s) 334, 427, 982, 1186, 1638
BspFNI CGCG 1 cut(s) 877
BspHI TCATGA 3 cut(s) 589, 819, 2470
BspLI GGNNCC 7 cut(s) 1113, 1322, 1640, 2134, 2144, 2215, 2675
BspPI GGATC 3 cut(s) 1849, 2137, 2150
BspT107I GGYRCC 4 cut(s) 1111, 1320, 1638, 2132
BsrDI GCAATG 5 cut(s) 86, 537, 751, 900, 1726
BsrFI RCCGGY 1 cut(s) 2092
BsrGI TGTACA 1 cut(s) 2418
BsrI ACTGG 5 cut(s) 723, 1242, 1701, 1891, 2631
BssAI RCCGGY 1 cut(s) 2092
BssECI CCNNGG 4 cut(s) 1584, 1642, 2007, 2041
BssNAI GTATAC 1 cut(s) 2509
BssSI CACGAG 1 cut(s) 504
BssT1I CCWWGG 2 cut(s) 1642, 2007
Bst1107I GTATAC 1 cut(s) 2509
Bst2BI CACGAG 1 cut(s) 504
Bst2UI CCWGG 4 cut(s) 137, 644, 1585, 2042
Bst4CI ACNGT 7 cut(s) 479, 1027, 1216, 1426, 1923, 2407, 2516
Bst6I CTCTTC 1 cut(s) 52
BstAPI GCANNNNNTGC 2 cut(s) 1040, 1058
BstAUI TGTACA 1 cut(s) 2418
BstC8I GCNNGC 6 cut(s) 133, 306, 1305, 1536, 1633, 2090
BstDEI CTNAG 5 cut(s) 342, 435, 969, 1194, 1625
BstF5I GGATG 7 cut(s) 198, 555, 898, 1462, 1501, 1967, 2503
BstFNI CGCG 1 cut(s) 877
BstHHI GCGC 2 cut(s) 1931, 2646
BstMAI GTCTC 3 cut(s) 156, 1413, 2632
BstMCI CGRYCG 1 cut(s) 118
BstNI CCWGG 4 cut(s) 137, 644, 1585, 2042
BstNSI RCATGY 4 cut(s) 308, 1260, 2092, 2257
BstSFI CTRYAG 1 cut(s) 2137
BstUI CGCG 1 cut(s) 877
BstV1I GCAGC 4 cut(s) 646, 853, 1108, 1145
BstV2I GAAGAC 1 cut(s) 734
BstX2I RGATCY 3 cut(s) 2074, 2142, 2207
BstXI CCANNNNNNTGG 1 cut(s) 1994
BstYI RGATCY 3 cut(s) 2074, 2142, 2207
BstZ17I GTATAC 1 cut(s) 2509
BstZI CGGCCG 1 cut(s) 115
BsuI GTATCC 1 cut(s) 2446
BsuRI GGCC 5 cut(s) 117, 637, 647, 1764, 2166
BtgZI GCGATG 1 cut(s) 965
BtsCI GGATG 7 cut(s) 198, 555, 898, 1462, 1501, 1967, 2503
BtsI GCAGTG 1 cut(s) 634
BtsIMutI CAGTG 8 cut(s) 634, 1212, 1249, 1633, 1919, 2154, 2521, 2638
Cac8I GCNNGC 6 cut(s) 133, 306, 1305, 1536, 1633, 2090
CaiI CAGNNNCTG 2 cut(s) 1214, 2578
CciI TCATGA 3 cut(s) 589, 819, 2470
CfoI GCGC 2 cut(s) 1931, 2646
Cfr10I RCCGGY 1 cut(s) 2092
Cfr13I GGNCC 1 cut(s) 270
CseI GACGC 1 cut(s) 929
DdeI CTNAG 5 cut(s) 342, 435, 969, 1194, 1625
EaeI YGGCCR 2 cut(s) 115, 2164
EagI CGGCCG 1 cut(s) 115
Eam1104I CTCTTC 1 cut(s) 52
EarI CTCTTC 1 cut(s) 52
Ecl136II GAGCTC 1 cut(s) 1186
EclXI CGGCCG 1 cut(s) 115
Eco130I CCWWGG 2 cut(s) 1642, 2007
Eco24I GRGCYC 2 cut(s) 173, 1188
Eco32I GATATC 1 cut(s) 1356
Eco47I GGWCC 1 cut(s) 270
Eco52I CGGCCG 1 cut(s) 115
Eco53kI GAGCTC 1 cut(s) 1186
Eco57I CTGAAG 2 cut(s) 2091, 2600
Eco88I CYCGRG 1 cut(s) 166
EcoICRI GAGCTC 1 cut(s) 1186
EcoRI GAATTC 1 cut(s) 457
EcoRII CCWGG 4 cut(s) 135, 642, 1583, 2040
EcoRV GATATC 1 cut(s) 1356
EcoT14I CCWWGG 2 cut(s) 1642, 2007
EcoT22I ATGCAT 1 cut(s) 2245
EcoT38I GRGCYC 2 cut(s) 173, 1188
ErhI CCWWGG 2 cut(s) 1642, 2007
FalI AAGNNNNNCTT 2 cut(s) 2112, 2144
FaqI GGGAC 2 cut(s) 428, 1602
FauI CCCGC 3 cut(s) 301, 1628, 2262
FauNDI CATATG 1 cut(s) 2536
FbaI TGATCA 1 cut(s) 424
FblI GTMKAC 2 cut(s) 1953, 2508
Fnu4HI GCNGC 4 cut(s) 660, 867, 1122, 1134
FokI GGATG 7 cut(s) 185, 562, 905, 1469, 1508, 1974, 2510
FriOI GRGCYC 2 cut(s) 173, 1188
Fsp4HI GCNGC 4 cut(s) 660, 867, 1122, 1134
FspBI CTAG 4 cut(s) 411, 653, 1401, 2457
GlaI GCGC 2 cut(s) 1930, 2645
GluI GCNGC 4 cut(s) 660, 867, 1122, 1134
GsaI CCCAGC 1 cut(s) 1947
GsuI CTGGAG 2 cut(s) 1202, 2614
HaeIII GGCC 5 cut(s) 117, 637, 647, 1764, 2166
HapII CCGG 8 cut(s) 285, 471, 1109, 1539, 1824, 2093, 2316, 2450
HgaI GACGC 1 cut(s) 929
HhaI GCGC 2 cut(s) 1931, 2646
Hin6I GCGC 2 cut(s) 1929, 2644
HinP1I GCGC 2 cut(s) 1929, 2644
HindIII AAGCTT 3 cut(s) 1395, 1470, 1655
HpaII CCGG 8 cut(s) 285, 471, 1109, 1539, 1824, 2093, 2316, 2450
HphI GGTGA 6 cut(s) 323, 593, 820, 1231, 2612, 2720
Hpy166II GTNNAC 5 cut(s) 757, 941, 1954, 2350, 2509
Hpy8I GTNNAC 5 cut(s) 757, 941, 1954, 2350, 2509
HpyAV CCTTC 1 cut(s) 1952
HpyCH4III ACNGT 7 cut(s) 479, 1027, 1216, 1426, 1923, 2407, 2516
HpyCH4IV ACGT 2 cut(s) 417, 1451
HpyF3I CTNAG 5 cut(s) 342, 435, 969, 1194, 1625
HpySE526I ACGT 2 cut(s) 417, 1451
HspAI GCGC 2 cut(s) 1929, 2644
KpnI GGTACC 3 cut(s) 1115, 1324, 1642
Ksp22I TGATCA 1 cut(s) 424
LmnI GCTCC 3 cut(s) 624, 1183, 2485
Lsp1109I GCAGC 4 cut(s) 646, 853, 1108, 1145
LweI GCATC 6 cut(s) 118, 793, 886, 1450, 1773, 2252
MaeI CTAG 4 cut(s) 411, 653, 1401, 2457
MaeII ACGT 2 cut(s) 417, 1451
MaeIII GTNAC 4 cut(s) 955, 1801, 2302, 2600
MboII GAAGA 8 cut(s) 39, 739, 812, 1091, 2084, 2459, 2593, 2699
MflI RGATCY 3 cut(s) 2074, 2142, 2207
MhlI GDGCHC 5 cut(s) 173, 1188, 1249, 1834, 2702
MlsI TGGCCA 1 cut(s) 2166
MluNI TGGCCA 1 cut(s) 2166
MlyI GAGTC 2 cut(s) 158, 341
MmeI TCCRAC 3 cut(s) 870, 1434, 2306
Mox20I TGGCCA 1 cut(s) 2166
Mph1103I ATGCAT 1 cut(s) 2245
MroXI GAANNNNTTC 3 cut(s) 815, 1668, 2124
MscI TGGCCA 1 cut(s) 2166
MseI TTAA 5 cut(s) 122, 401, 495, 710, 1311
MslI CAYNNNNRTG 4 cut(s) 303, 309, 824, 1924
Msp20I TGGCCA 1 cut(s) 2166
MspA1I CMGCKG 1 cut(s) 671
MspI CCGG 8 cut(s) 285, 471, 1109, 1539, 1824, 2093, 2316, 2450
Mva1269I GAATGC 7 cut(s) 156, 370, 778, 831, 1045, 1077, 1816
MvaI CCWGG 4 cut(s) 137, 644, 1585, 2042
MvnI CGCG 1 cut(s) 877
NciI CCSGG 6 cut(s) 286, 472, 1109, 1540, 1825, 2316
NdeI CATATG 1 cut(s) 2536
NlaIV GGNNCC 7 cut(s) 1113, 1322, 1640, 2134, 2144, 2215, 2675
NmeAIII GCCGAG 1 cut(s) 93
NmuCI GTSAC 3 cut(s) 1801, 2302, 2600
NsiI ATGCAT 1 cut(s) 2245
NspI RCATGY 4 cut(s) 308, 1260, 2092, 2257
PaeI GCATGC 2 cut(s) 308, 2092
PagI TCATGA 3 cut(s) 589, 819, 2470
PctI GAATGC 7 cut(s) 156, 370, 778, 831, 1045, 1077, 1816
PdmI GAANNNNTTC 3 cut(s) 815, 1668, 2124
PkrI GCNGC 4 cut(s) 661, 868, 1123, 1135
PleI GAGTC 2 cut(s) 158, 340
PpsI GAGTC 2 cut(s) 158, 340
PshBI ATTAAT 1 cut(s) 495
Psp124BI GAGCTC 1 cut(s) 1188
Psp6I CCWGG 4 cut(s) 135, 642, 1583, 2040
PspFI CCCAGC 1 cut(s) 1943
PspGI CCWGG 4 cut(s) 135, 642, 1583, 2040
PspN4I GGNNCC 7 cut(s) 1113, 1322, 1640, 2134, 2144, 2215, 2675
PspPI GGNCC 1 cut(s) 270
PstNI CAGNNNCTG 2 cut(s) 1214, 2578
PsuI RGATCY 3 cut(s) 2074, 2142, 2207
PvuII CAGCTG 1 cut(s) 671
RseI CAYNNNNRTG 4 cut(s) 303, 309, 824, 1924
SacI GAGCTC 1 cut(s) 1188
SaqAI TTAA 5 cut(s) 122, 401, 495, 710, 1311
SatI GCNGC 4 cut(s) 660, 867, 1122, 1134
Sau96I GGNCC 1 cut(s) 270
SchI GAGTC 2 cut(s) 158, 341
SduI GDGCHC 5 cut(s) 173, 1188, 1249, 1834, 2702
SfaNI GCATC 6 cut(s) 118, 793, 886, 1450, 1773, 2252
SfcI CTRYAG 1 cut(s) 2137
SinI GGWCC 1 cut(s) 270
SmiMI CAYNNNNRTG 4 cut(s) 303, 309, 824, 1924
SmlI CTYRAG 1 cut(s) 318
SmoI CTYRAG 1 cut(s) 318
SpeI ACTAGT 2 cut(s) 652, 2456
SphI GCATGC 2 cut(s) 308, 2092
SsiI CCGC 8 cut(s) 308, 323, 486, 806, 877, 1635, 2269, 2598
SspI AATATT 1 cut(s) 768
SspMI CTAG 4 cut(s) 411, 653, 1401, 2457
SstI GAGCTC 1 cut(s) 1188
StyI CCWWGG 2 cut(s) 1642, 2007
TaaI ACNGT 7 cut(s) 479, 1027, 1216, 1426, 1923, 2407, 2516
TaiI ACGT 2 cut(s) 420, 1454
TaqI TCGA 5 cut(s) 516, 854, 1465, 1792, 2149
TatI WGTACW 3 cut(s) 1216, 1737, 2418
Tru1I TTAA 5 cut(s) 122, 401, 495, 710, 1311
Tru9I TTAA 5 cut(s) 122, 401, 495, 710, 1311
TscAI CASTG 8 cut(s) 634, 1219, 1249, 1633, 1926, 2161, 2521, 2638
TseFI GTSAC 3 cut(s) 1801, 2302, 2600
TseI GCWGC 4 cut(s) 659, 866, 1121, 1133
Tsp45I GTSAC 3 cut(s) 1801, 2302, 2600
TspGWI ACGGA 5 cut(s) 56, 1176, 1854, 2098, 2218
TspRI CASTG 8 cut(s) 634, 1219, 1249, 1633, 1926, 2161, 2521, 2638
VpaK11BI GGWCC 1 cut(s) 270
VspI ATTAAT 1 cut(s) 495
XapI RAATTY 3 cut(s) 457, 1234, 2621
XceI RCATGY 4 cut(s) 308, 1260, 2092, 2257
XcmI CCANNNNNNNNNTGG 1 cut(s) 2687
XmiI GTMKAC 2 cut(s) 1953, 2508
XmnI GAANNNNTTC 3 cut(s) 815, 1668, 2124
XspI CTAG 4 cut(s) 411, 653, 1401, 2457
Zsp2I ATGCAT 1 cut(s) 2245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.