MD17G1244700.v1.1

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0020269
Physical Location & Seq
Forward (+)
29307776 .. 29319387
11612 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1244700.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGGCTGCTGTCCTCTCCCTCCGCACTATCTCCCAACTTCATCCCAACCTCTCTTCCCGGAGGGTTACCATAAAGCCCAGGGCAGCCCTCCATGGAGCCCGCAGAATGCGAGTCCCTTACCAGCTCAAACAGGAACAATCCCGTCTTTTCCACCGGCTCCCCTCCGGCCTGAACATGGAGGTAATCGTGCACAAAAGAGTTGCAGAAAAGCAATCAGACGAGGAAAAAGAGAGACCAAGTGAAAACCCACCTCTAGTTTTCGTCCATGGAAGCTACCACGCTGCTTGGTGCTGGGCTGAGCACTGGCTCCCTTTCTTTTCGGCTTCTGGGTATGATTGCTATGCAGTTAGCTTGTTGGGTCAGGGTGAAAGTGATGCACCCAGCGCTTCTGTTTCTGGTACTCTCCAGACGCATGCAAGTGATGTTGCTGATTTCATCTGCAAGAAACTCACGCTTCCACCAGTATTGATTGGACACTCTTTTGGAGGGCTTATTATTCAATACTATATTGCAAACGCTAAAACTAACCAGATTTCAGACAAGAGAGATTTGTTCCCTAAGCTTACTGGTGCTGTGCTTGTCTGCTCCGTACCTCCTTCGGGTAATAGTGGCTTAGTGTGGCGTTATCTCTTCACCAAACCTATTGCTGCTTTTAAGGTTACACGCAGCTTGGCAGCTAAAGGGTTTCAAACATCTCTTTCTCTTTGTAAGGAGACATTTTTTTCTGCAACAATGGAGGATCACCTCGTTTTACGCTATCAAGAGCTAATGAAGGAAAGTTCAAGAATGCCATTGTTTGATCTGCGGAAGCTCAATGCATCGCTGCCAGTTCCTTCAGTGCCAAAATCAGCTATTGAACTCCTCGTGTTGGGTGCAAATGATGATTTCATTGTGGATGCTGAAGGCCTCAACGAGACAGGCAGGTTTTATGGTGTCTCTCCTATCTGTGTTAAAGGGGTTGCCCATGACATGATGTTGGATTGTTTACGGGAGAAGGGTGCAAAAGCTATCCTATCATGGCTCGAAGATTTGAAGAGAGAGCAACTTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

351

Amino Acids

38.95

Weight (kDa)

9.08

Isoelectric Point (pI)

45.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 83 - 173 2e-16 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 85 - 326 9.7e-22 Alpha/beta hydrolase family
Hydrolase_4 PF12146 85 - 175 5.9e-08 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0020269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38360 AT5G38360 AT5G38360
malus_domestica MD17G1244700.v1.1
prunus_persica Prupe.3G142000_v2.0.a1
pyrus_communis pycom17g24750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 912
AciI CCGC 3 cut(s) 22, 100, 805
AclWI GGATC 1 cut(s) 747
AcuI CTGAAG 2 cut(s) 819, 921
AfaI GTAC 2 cut(s) 400, 591
AfeI AGCGCT 1 cut(s) 385
AfiI CCNNNNNNNGG 3 cut(s) 175, 599, 868
AgsI TTSAA 5 cut(s) 500, 689, 783, 857, 1033
AjnI CCWGG 1 cut(s) 77
Alw21I GWGCWC 2 cut(s) 192, 303
Alw26I GTCTC 4 cut(s) 226, 707, 908, 940
Alw44I GTGCAC 1 cut(s) 188
AlwI GGATC 1 cut(s) 747
Aor51HI AGCGCT 1 cut(s) 385
AoxI GGCC 2 cut(s) 166, 904
ApaLI GTGCAC 1 cut(s) 188
ApeKI GCWGC 7 cut(s) 5, 83, 281, 647, 666, 674, 823
AspLEI GCGC 1 cut(s) 386
AsuC2I CCSGG 1 cut(s) 58
AsuHPI GGTGA 3 cut(s) 377, 625, 734
BaeGI GKGCMC 1 cut(s) 192
BanII GRGCYC 1 cut(s) 100
BauI CACGAG 1 cut(s) 863
Bbv12I GWGCWC 2 cut(s) 192, 303
BbvI GCAGC 6 cut(s) 95, 268, 634, 678, 686, 810
BciT130I CCWGG 1 cut(s) 79
BcnI CCSGG 1 cut(s) 58
BcoDI GTCTC 4 cut(s) 226, 707, 908, 940
BfaI CTAG 1 cut(s) 254
BfoI RGCGCY 1 cut(s) 387
BfuAI ACCTGC 1 cut(s) 912
BisI GCNGC 7 cut(s) 6, 84, 282, 648, 667, 675, 824
BlpI GCTNAGC 1 cut(s) 297
BlsI GCNGC 7 cut(s) 7, 85, 283, 649, 668, 676, 825
Bme1390I CCNGG 2 cut(s) 58, 79
BmiI GGNNCC 3 cut(s) 97, 158, 308
BmrFI CCNGG 2 cut(s) 58, 79
BmsI GCATC 3 cut(s) 364, 827, 886
BpmI CTGGAG 1 cut(s) 389
Bpu10I CCTNAGC 1 cut(s) 558
Bpu1102I GCTNAGC 1 cut(s) 297
BpuMI CCSGG 1 cut(s) 58
BsaI GGTCTC 1 cut(s) 226
BsaJI CCNNGG 4 cut(s) 77, 78, 91, 265
Bsc4I CCNNNNNNNGG 3 cut(s) 175, 599, 868
Bse118I RCCGGY 1 cut(s) 153
Bse1I ACTGG 4 cut(s) 308, 461, 571, 827
BseBI CCWGG 1 cut(s) 79
BseDI CCNNGG 4 cut(s) 77, 78, 91, 265
BseGI GGATG 2 cut(s) 40, 901
BseLI CCNNNNNNNGG 3 cut(s) 175, 599, 868
BseMII CTCAG 1 cut(s) 288
BseNI ACTGG 4 cut(s) 308, 461, 571, 827
BseRI GAGGAG 1 cut(s) 851
BseSI GKGCMC 1 cut(s) 192
BseXI GCAGC 6 cut(s) 95, 268, 634, 678, 686, 810
BseYI CCCAGC 2 cut(s) 291, 380
BshFI GGCC 2 cut(s) 168, 906
BsiHKAI GWGCWC 2 cut(s) 192, 303
BsiSI CCGG 3 cut(s) 58, 154, 165
BslFI GGGAC 1 cut(s) 98
BslI CCNNNNNNNGG 3 cut(s) 175, 599, 868
BsmAI GTCTC 4 cut(s) 226, 707, 908, 940
BsmFI GGGAC 1 cut(s) 98
BsmI GAATGC 2 cut(s) 111, 792
BsnI GGCC 2 cut(s) 168, 906
Bso31I GGTCTC 1 cut(s) 226
Bsp1286I GDGCHC 3 cut(s) 100, 192, 303
Bsp143I GATC 2 cut(s) 739, 799
Bsp1720I GCTNAGC 1 cut(s) 297
Bsp19I CCATGG 2 cut(s) 91, 265
BspACI CCGC 3 cut(s) 22, 100, 805
BspANI GGCC 2 cut(s) 168, 906
BspCNI CTCAG 1 cut(s) 289
BspLI GGNNCC 3 cut(s) 97, 158, 308
BspMI ACCTGC 1 cut(s) 912
BspPI GGATC 1 cut(s) 747
BspTNI GGTCTC 1 cut(s) 226
BsrFI RCCGGY 1 cut(s) 153
BsrI ACTGG 4 cut(s) 308, 461, 571, 827
BssAI RCCGGY 1 cut(s) 153
BssECI CCNNGG 4 cut(s) 77, 78, 91, 265
BssMI GATC 2 cut(s) 739, 799
BssSI CACGAG 1 cut(s) 863
BssT1I CCWWGG 2 cut(s) 91, 265
Bst2BI CACGAG 1 cut(s) 863
Bst2UI CCWGG 1 cut(s) 79
Bst6I CTCTTC 3 cut(s) 58, 635, 1028
BstC8I GCNNGC 2 cut(s) 100, 414
BstDEI CTNAG 4 cut(s) 297, 558, 613, 1046
BstDSI CCRYGG 2 cut(s) 91, 265
BstEII GGTNACC 1 cut(s) 64
BstF5I GGATG 2 cut(s) 40, 901
BstH2I RGCGCY 1 cut(s) 387
BstHHI GCGC 1 cut(s) 386
BstKTI GATC 2 cut(s) 742, 802
BstMAI GTCTC 4 cut(s) 226, 707, 908, 940
BstMBI GATC 2 cut(s) 739, 799
BstMWI GCNNNNNNNGC 1 cut(s) 383
BstNI CCWGG 1 cut(s) 79
BstNSI RCATGY 1 cut(s) 416
BstPI GGTNACC 1 cut(s) 64
BstSCI CCNGG 2 cut(s) 56, 77
BstSLI GKGCMC 1 cut(s) 192
BstV1I GCAGC 6 cut(s) 95, 268, 634, 678, 686, 810
BsuRI GGCC 2 cut(s) 168, 906
BtgI CCRYGG 2 cut(s) 91, 265
BtgZI GCGATG 1 cut(s) 804
BtsCI GGATG 2 cut(s) 40, 901
BtsIMutI CAGTG 2 cut(s) 301, 843
BveI ACCTGC 1 cut(s) 912
Cac8I GCNNGC 2 cut(s) 100, 414
CfoI GCGC 1 cut(s) 386
Cfr10I RCCGGY 1 cut(s) 153
CseI GACGC 1 cut(s) 418
Csp6I GTAC 2 cut(s) 399, 590
CspCI CAANNNNNGTGG 4 cut(s) 266, 301, 447, 482
CviAII CATG 7 cut(s) 92, 175, 266, 413, 965, 970, 1017
CviQI GTAC 2 cut(s) 399, 590
DdeI CTNAG 4 cut(s) 297, 558, 613, 1046
DpnI GATC 2 cut(s) 741, 801
DpnII GATC 2 cut(s) 739, 799
Eam1104I CTCTTC 3 cut(s) 58, 635, 1028
EarI CTCTTC 3 cut(s) 58, 635, 1028
Eco130I CCWWGG 2 cut(s) 91, 265
Eco147I AGGCCT 1 cut(s) 906
Eco24I GRGCYC 1 cut(s) 100
Eco31I GGTCTC 1 cut(s) 226
Eco47III AGCGCT 1 cut(s) 385
Eco57I CTGAAG 2 cut(s) 819, 921
Eco91I GGTNACC 1 cut(s) 64
EcoO65I GGTNACC 1 cut(s) 64
EcoRII CCWGG 1 cut(s) 77
EcoT14I CCWWGG 2 cut(s) 91, 265
EcoT22I ATGCAT 1 cut(s) 820
EcoT38I GRGCYC 1 cut(s) 100
ErhI CCWWGG 2 cut(s) 91, 265
FaeI CATG 7 cut(s) 95, 178, 269, 416, 968, 973, 1020
FaqI GGGAC 1 cut(s) 98
FatI CATG 7 cut(s) 91, 174, 265, 412, 964, 969, 1016
FauI CCCGC 1 cut(s) 107
Fnu4HI GCNGC 7 cut(s) 6, 84, 282, 648, 667, 675, 824
FokI GGATG 2 cut(s) 27, 908
FriOI GRGCYC 1 cut(s) 100
Fsp4HI GCNGC 7 cut(s) 6, 84, 282, 648, 667, 675, 824
FspBI CTAG 1 cut(s) 254
GlaI GCGC 1 cut(s) 385
GluI GCNGC 7 cut(s) 6, 84, 282, 648, 667, 675, 824
GsaI CCCAGC 2 cut(s) 295, 384
GsuI CTGGAG 1 cut(s) 389
HaeII RGCGCY 1 cut(s) 387
HaeIII GGCC 2 cut(s) 168, 906
HapII CCGG 3 cut(s) 58, 154, 165
HgaI GACGC 1 cut(s) 418
HhaI GCGC 1 cut(s) 386
Hin1II CATG 7 cut(s) 95, 178, 269, 416, 968, 973, 1020
Hin6I GCGC 1 cut(s) 384
HinP1I GCGC 1 cut(s) 384
HindIII AAGCTT 1 cut(s) 560
HinfI GANTC 1 cut(s) 111
HpaII CCGG 3 cut(s) 58, 154, 165
HphI GGTGA 3 cut(s) 377, 625, 734
Hpy166II GTNNAC 2 cut(s) 190, 986
Hpy188I TCNGA 2 cut(s) 217, 538
Hpy188III TCNNGA 3 cut(s) 406, 761, 783
Hpy8I GTNNAC 2 cut(s) 190, 986
HpyAV CCTTC 5 cut(s) 606, 766, 843, 896, 988
HpyF10VI GCNNNNNNNGC 1 cut(s) 383
HpyF3I CTNAG 4 cut(s) 297, 558, 613, 1046
Hsp92II CATG 7 cut(s) 95, 178, 269, 416, 968, 973, 1020
HspAI GCGC 1 cut(s) 384
Kzo9I GATC 2 cut(s) 739, 799
LmnI GCTCC 4 cut(s) 95, 162, 312, 590
Lsp1109I GCAGC 6 cut(s) 95, 268, 634, 678, 686, 810
LweI GCATC 3 cut(s) 364, 827, 886
MaeI CTAG 1 cut(s) 254
MaeIII GTNAC 2 cut(s) 64, 658
MalI GATC 2 cut(s) 741, 801
MboI GATC 2 cut(s) 739, 799
MboII GAAGA 4 cut(s) 45, 622, 1037, 1045
MhlI GDGCHC 3 cut(s) 100, 192, 303
MlyI GAGTC 1 cut(s) 120
MmeI TCCRAC 1 cut(s) 957
Mph1103I ATGCAT 1 cut(s) 820
MseI TTAA 2 cut(s) 654, 951
MslI CAYNNNNRTG 1 cut(s) 417
MspI CCGG 3 cut(s) 58, 154, 165
MspR9I CCNGG 2 cut(s) 58, 79
Mva1269I GAATGC 2 cut(s) 111, 792
MvaI CCWGG 1 cut(s) 79
MwoI GCNNNNNNNGC 1 cut(s) 383
NciI CCSGG 1 cut(s) 58
NcoI CCATGG 2 cut(s) 91, 265
NdeII GATC 2 cut(s) 739, 799
NlaIII CATG 7 cut(s) 95, 178, 269, 416, 968, 973, 1020
NlaIV GGNNCC 3 cut(s) 97, 158, 308
NsiI ATGCAT 1 cut(s) 820
NspI RCATGY 1 cut(s) 416
PaeI GCATGC 1 cut(s) 416
PasI CCCWGGG 1 cut(s) 78
PceI AGGCCT 1 cut(s) 906
PctI GAATGC 2 cut(s) 111, 792
PfoI TCCNGGA 1 cut(s) 56
PkrI GCNGC 7 cut(s) 7, 85, 283, 649, 668, 676, 825
PleI GAGTC 1 cut(s) 119
PpsI GAGTC 1 cut(s) 119
Psp6I CCWGG 1 cut(s) 77
PspEI GGTNACC 1 cut(s) 64
PspFI CCCAGC 2 cut(s) 291, 380
PspGI CCWGG 1 cut(s) 77
PspN4I GGNNCC 3 cut(s) 97, 158, 308
RsaI GTAC 2 cut(s) 400, 591
RsaNI GTAC 2 cut(s) 399, 590
RseI CAYNNNNRTG 1 cut(s) 417
SaqAI TTAA 2 cut(s) 654, 951
SatI GCNGC 7 cut(s) 6, 84, 282, 648, 667, 675, 824
Sau3AI GATC 2 cut(s) 739, 799
SchI GAGTC 1 cut(s) 120
ScrFI CCNGG 2 cut(s) 58, 79
SduI GDGCHC 3 cut(s) 100, 192, 303
SfaNI GCATC 3 cut(s) 364, 827, 886
SmiMI CAYNNNNRTG 1 cut(s) 417
SphI GCATGC 1 cut(s) 416
SseBI AGGCCT 1 cut(s) 906
SsiI CCGC 3 cut(s) 22, 100, 805
SspMI CTAG 1 cut(s) 254
StuI AGGCCT 1 cut(s) 906
StyD4I CCNGG 2 cut(s) 56, 77
StyI CCWWGG 2 cut(s) 91, 265
TaqI TCGA 1 cut(s) 1023
Tru1I TTAA 2 cut(s) 654, 951
Tru9I TTAA 2 cut(s) 654, 951
TscAI CASTG 2 cut(s) 308, 843
TseI GCWGC 7 cut(s) 5, 83, 281, 647, 666, 674, 823
TspDTI ATGAA 4 cut(s) 29, 424, 785, 877
TspGWI ACGGA 1 cut(s) 577
TspRI CASTG 2 cut(s) 308, 843
VneI GTGCAC 1 cut(s) 188
XceI RCATGY 1 cut(s) 416
XspI CTAG 1 cut(s) 254
Zsp2I ATGCAT 1 cut(s) 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.