Prupe.1G063200_v2.0.a1

3-beta hydroxysteroid dehydrogenase/isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0021248
Physical Location & Seq
Forward (+)
4482296 .. 4483189
894 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G063200.1

Sequence Viewer

Length: 483 bp
ATGCCTATCAAAGAACTTGATGAAGAAGCAGTGACCAAAAAAGCTGTCCAAGGAGCCCTGGGCATATTGAAAGCCTGCCTAAATTCCAAGACTGTGAAGAGGGTTGTGTACACTTCTAGTGCATCAACTGTGGCTTACAGTGGCAGCAGCCAAGACTTGGTGGATGAGAGCTCATGGAGTGATATTGAATTTCATAGGTCTCTTAAAATATTTGGAACTTCATATGTTGCTGCCAAGACCAAAACAGAGCAAGCTATCCTGGAATTTGCAGAAATTGGGTGGAGGAAGAAATCTGGGGGGGAGGGAAGAAATTGGGAGGAAGGGAGAATCTGGGTAGGGTTTTTATTTTATTTCTTAATTTTTAATTTAATATGTTTAATTTTTTATAATAATTTAAAAATACAATTTTTATTAGTTTTTTATTCACGTGAAACCTCACGTCAATTAAAAAATAGATCCTACAAATATCCCATCAGTATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

161

Amino Acids

18.32

Weight (kDa)

9.3

Isoelectric Point (pI)

47.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021248)

Species Orthologous Gene IDs
prunus_persica Prupe.1G063200_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0350301
rosa_rugosa Rorug01G0210800
rosa_samantha Rh1BG193500 Rh1DG221800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 387
AccB7I CCANNNNNTGG 1 cut(s) 157
AclWI GGATC 1 cut(s) 450
AcsI RAATTY 3 cut(s) 82, 188, 263
AcvI CACGTG 1 cut(s) 428
AfaI GTAC 1 cut(s) 110
AfiI CCNNNNNNNGG 1 cut(s) 157
AgsI TTSAA 2 cut(s) 70, 188
AjiI CACGTC 1 cut(s) 440
AjnI CCWGG 2 cut(s) 57, 258
AluBI AGCT 3 cut(s) 44, 171, 254
AluI AGCT 3 cut(s) 44, 171, 254
Alw21I GWGCWC 1 cut(s) 173
Alw26I GTCTC 1 cut(s) 204
AlwI GGATC 1 cut(s) 450
ApeKI GCWGC 3 cut(s) 144, 147, 230
ApoI RAATTY 3 cut(s) 82, 188, 263
ArsI GACNNNNNNTTYG 2 cut(s) 30, 62
BanII GRGCYC 2 cut(s) 58, 173
BbrPI CACGTG 1 cut(s) 428
Bbv12I GWGCWC 1 cut(s) 173
BbvI GCAGC 3 cut(s) 156, 159, 217
BccI CCATC 1 cut(s) 479
BciT130I CCWGG 2 cut(s) 59, 260
BcoDI GTCTC 1 cut(s) 204
BfaI CTAG 1 cut(s) 117
BisI GCNGC 3 cut(s) 145, 148, 231
BlsI GCNGC 3 cut(s) 146, 149, 232
Bme1390I CCNGG 2 cut(s) 59, 260
BmgBI CACGTC 1 cut(s) 440
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 2 cut(s) 59, 260
BmsI GCATC 1 cut(s) 131
BsaAI YACGTR 1 cut(s) 428
BsaI GGTCTC 1 cut(s) 204
BsaJI CCNNGG 3 cut(s) 49, 57, 58
Bsc4I CCNNNNNNNGG 1 cut(s) 157
BseBI CCWGG 2 cut(s) 59, 260
BseDI CCNNGG 3 cut(s) 49, 57, 58
BseGI GGATG 1 cut(s) 169
BseLI CCNNNNNNNGG 1 cut(s) 157
BseXI GCAGC 3 cut(s) 156, 159, 217
BsiHKAI GWGCWC 1 cut(s) 173
BslI CCNNNNNNNGG 1 cut(s) 157
BsmAI GTCTC 1 cut(s) 204
Bso31I GGTCTC 1 cut(s) 204
Bsp1286I GDGCHC 2 cut(s) 58, 173
Bsp1407I TGTACA 1 cut(s) 108
Bsp143I GATC 1 cut(s) 455
BspLI GGNNCC 1 cut(s) 55
BspPI GGATC 1 cut(s) 450
BspTNI GGTCTC 1 cut(s) 204
BsrGI TGTACA 1 cut(s) 108
BssECI CCNNGG 3 cut(s) 49, 57, 58
BssMI GATC 1 cut(s) 455
BssT1I CCWWGG 1 cut(s) 49
Bst2UI CCWGG 2 cut(s) 59, 260
Bst4CI ACNGT 3 cut(s) 94, 130, 140
Bst6I CTCTTC 1 cut(s) 92
BstAUI TGTACA 1 cut(s) 108
BstBAI YACGTR 1 cut(s) 428
BstC8I GCNNGC 2 cut(s) 76, 252
BstF5I GGATG 1 cut(s) 169
BstKTI GATC 1 cut(s) 458
BstMAI GTCTC 1 cut(s) 204
BstMBI GATC 1 cut(s) 455
BstNI CCWGG 2 cut(s) 59, 260
BstSCI CCNGG 2 cut(s) 57, 258
BstV1I GCAGC 3 cut(s) 156, 159, 217
BstX2I RGATCY 1 cut(s) 455
BstYI RGATCY 1 cut(s) 455
BtrI CACGTC 1 cut(s) 440
BtsCI GGATG 1 cut(s) 169
BtsI GCAGTG 1 cut(s) 36
BtsIMutI CAGTG 2 cut(s) 36, 145
Cac8I GCNNGC 2 cut(s) 76, 252
Csp6I GTAC 1 cut(s) 109
CviAII CATG 1 cut(s) 174
CviJI RGCY 7 cut(s) 44, 56, 74, 134, 150, 171, 254
CviKI_1 RGCY 7 cut(s) 44, 56, 74, 134, 150, 171, 254
CviQI GTAC 1 cut(s) 109
DpnI GATC 1 cut(s) 457
DpnII GATC 1 cut(s) 455
DraI TTTAAA 1 cut(s) 396
Eam1104I CTCTTC 1 cut(s) 92
EarI CTCTTC 1 cut(s) 92
Ecl136II GAGCTC 1 cut(s) 171
Eco130I CCWWGG 1 cut(s) 49
Eco24I GRGCYC 2 cut(s) 58, 173
Eco31I GGTCTC 1 cut(s) 204
Eco53kI GAGCTC 1 cut(s) 171
Eco72I CACGTG 1 cut(s) 428
EcoICRI GAGCTC 1 cut(s) 171
EcoRII CCWGG 2 cut(s) 57, 258
EcoT14I CCWWGG 1 cut(s) 49
EcoT38I GRGCYC 2 cut(s) 58, 173
ErhI CCWWGG 1 cut(s) 49
FaeI CATG 1 cut(s) 177
FaiI YATR 7 cut(s) 65, 175, 195, 223, 225, 373, 387
FatI CATG 1 cut(s) 173
FauNDI CATATG 1 cut(s) 223
Fnu4HI GCNGC 3 cut(s) 145, 148, 231
FokI GGATG 1 cut(s) 176
FriOI GRGCYC 2 cut(s) 58, 173
Fsp4HI GCNGC 3 cut(s) 145, 148, 231
FspBI CTAG 1 cut(s) 117
GluI GCNGC 3 cut(s) 145, 148, 231
Hin1II CATG 1 cut(s) 177
HinfI GANTC 1 cut(s) 327
Hpy166II GTNNAC 2 cut(s) 109, 111
Hpy8I GTNNAC 2 cut(s) 109, 111
HpyAV CCTTC 1 cut(s) 314
HpyCH4III ACNGT 3 cut(s) 94, 130, 140
HpyCH4IV ACGT 2 cut(s) 427, 439
HpyCH4V TGCA 2 cut(s) 122, 269
HpySE526I ACGT 2 cut(s) 427, 439
Hsp92II CATG 1 cut(s) 177
Kzo9I GATC 1 cut(s) 455
LmnI GCTCC 1 cut(s) 53
LpnPI CCDG 7 cut(s) 44, 71, 88, 245, 272, 279, 316
Lsp1109I GCAGC 3 cut(s) 156, 159, 217
LweI GCATC 1 cut(s) 131
MaeI CTAG 1 cut(s) 117
MaeII ACGT 2 cut(s) 427, 439
MaeIII GTNAC 1 cut(s) 31
MalI GATC 1 cut(s) 457
MboI GATC 1 cut(s) 455
MboII GAAGA 4 cut(s) 35, 109, 298, 318
MflI RGATCY 1 cut(s) 455
MhlI GDGCHC 2 cut(s) 58, 173
MnlI CCTC 5 cut(s) 93, 276, 295, 310, 445
MseI TTAA 7 cut(s) 204, 356, 363, 368, 377, 395, 446
MspR9I CCNGG 2 cut(s) 59, 260
MvaI CCWGG 2 cut(s) 59, 260
NdeI CATATG 1 cut(s) 223
NdeII GATC 1 cut(s) 455
NlaIII CATG 1 cut(s) 177
NlaIV GGNNCC 1 cut(s) 55
NmuCI GTSAC 1 cut(s) 31
PasI CCCWGGG 1 cut(s) 58
PfeI GAWTC 1 cut(s) 327
PflMI CCANNNNNTGG 1 cut(s) 157
PfoI TCCNGGA 1 cut(s) 258
PkrI GCNGC 3 cut(s) 146, 149, 232
PmaCI CACGTG 1 cut(s) 428
PmlI CACGTG 1 cut(s) 428
Ppu21I YACGTR 1 cut(s) 428
PsiI TTATAA 1 cut(s) 387
Psp124BI GAGCTC 1 cut(s) 173
Psp6I CCWGG 2 cut(s) 57, 258
PspCI CACGTG 1 cut(s) 428
PspGI CCWGG 2 cut(s) 57, 258
PspN4I GGNNCC 1 cut(s) 55
PsuI RGATCY 1 cut(s) 455
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
SacI GAGCTC 1 cut(s) 173
SaqAI TTAA 7 cut(s) 204, 356, 363, 368, 377, 395, 446
SatI GCNGC 3 cut(s) 145, 148, 231
Sau3AI GATC 1 cut(s) 455
ScrFI CCNGG 2 cut(s) 59, 260
SduI GDGCHC 2 cut(s) 58, 173
SetI ASST 7 cut(s) 46, 173, 200, 256, 430, 437, 442
SfaNI GCATC 1 cut(s) 131
SspI AATATT 1 cut(s) 210
SspMI CTAG 1 cut(s) 117
SstI GAGCTC 1 cut(s) 173
StyD4I CCNGG 2 cut(s) 57, 258
StyI CCWWGG 1 cut(s) 49
TaaI ACNGT 3 cut(s) 94, 130, 140
TaiI ACGT 2 cut(s) 430, 442
TatI WGTACW 1 cut(s) 108
TfiI GAWTC 1 cut(s) 327
Tru1I TTAA 7 cut(s) 204, 356, 363, 368, 377, 395, 446
Tru9I TTAA 7 cut(s) 204, 356, 363, 368, 377, 395, 446
TscAI CASTG 2 cut(s) 36, 145
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 3 cut(s) 144, 147, 230
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 3 cut(s) 36, 182, 210
TspRI CASTG 2 cut(s) 36, 145
Van91I CCANNNNNTGG 1 cut(s) 157
XapI RAATTY 3 cut(s) 82, 188, 263
XspI CTAG 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.