Rorug01G0210800

3-beta hydroxysteroid dehydrogenase/isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000382
Physical Location & Seq
Reverse (-)
29827575 .. 29829897
2323 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0210800.1

Sequence Viewer

Length: 1125 bp
ATGATGATGAGCAATTATGGAGGCCAACGACTACTAGTGGTCGACTGTTGCTATTGCCAGACCCAAATCCAGCTGCCTTCTGTGAGTCCGATATACATCGTCGGCACATCCGTCCGCTGCGGCCGCTGCTATGCCAGTACCCACGTCGCCTCTCCGGGCTTCCCCCGCTCCCCCCATAGTGCCGTACCCTACTATGCCCTTATAAACCCACCACCACATGCCTGCTTCCCCCATACATCACCAGCACCTCCGCCGAACGTGCATGGGCGGAAGAAGGCGGTGATCTGCGGGATATCGTATAGAAACTCGAGGGATGAGCTCAATTGTTGCATCAATGACGCCAAATGCATGCGGTATCTCCTCATCACCAAGTTCAAGTTTCCAGAAGATTCCATTCTCATGCTCACTGAAGAAGAAACTCACCCACATAAGATTCCATATAAAAACAACATTAGAAGGGCATTATGTTGGCTTGTACAAGGATGTCAACCGGGTGACTCCCTTCTGTTTTACTTCTCTGGTCATGGTTCACGGCAGAGGAATTATCATGGTGATGAAGTTGATGGATATGATGAAACCCTTTGCCCCCTTGACTTCGAAACCCAGGGTATGATTGTTGATGATGAGATAAATGCAGCAATTGTTAGGCCCATTCCACATGGGGTCAAGCTTCATGCAATTATAGATTCTTGTCATAGTGGCACCATACTGGATTTGCCATGCCTTTGCAGAATGGACAGGGGTGGAAAATATGTATGGGAGGATCATCGCCCTCCATCAGGCGTATGGAAAGGAACAAGTGGTGGACAAGTTATATCTATCAGCGGTTGTGATGACCATCAAACCTCTGCGGAGACATCAATCACATCAACAGGTGCCATGACTTTCTCTTTCATCCAAGCAATCGAGCATGGACAGGCAGCCACATATGGGAGCTTACTCATATCTATGCGTAATACCATTCGAAGTATGGGTTCTGGTGGTGGTGGTAGTGGTGGTGCTGTTACATCTGTTGCCGTCATGCTTCTAACAGGCGGAAATGTTGGTGGTGGTAGGCTAAGACAGAAGCCTCAATTAACTGGCTGCGAGCAGTTCGATGTGTACACGAAACCTTTTGCCCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

374

Amino Acids

40.75

Weight (kDa)

7.52

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C14 PF00656 90 - 322 3.1e-31 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45400 AT2G45400 AT2G45400 AT2G45400
fragaria_vesca FvH4_7g11750 FvH4_7g11760 FvH4_7g11780 FvH4_7g11781 FvH4_7g11781 FvH4_7g11790 FvH4_7g11790
malus_domestica MD02G1205000.v1.1 MD07G1121700.v1.1 MD08G1231300.v1.1 MD08G1231400.v1.1 MD08G1231500.v1.1
prunus_persica Prupe.2G144600_v2.0.a1 Prupe.2G144700_v2.0.a1 Prupe.2G144800_v2.0.a1
pyrus_communis pycom08g20120
rosa_chinensis RchiOBHm_Chr1g0350271 RchiOBHm_Chr1g0350281 RchiOBHm_Chr1g0350311 RchiOBHm_Chr1g0350321 RchiOBHm_Chr1g0350341 RchiOBHm_Chr1g0350361 RchiOBHm_Chr1g0350371 RchiOBHm_Chr1g0350401 RchiOBHm_Chr1g0350411 RchiOBHm_Chr1g0350431 RchiOBHm_Chr1g0350441 RchiOBHm_Chr1g0350471
rosa_laevigata RLG00000028483 RLG00000028485 RLG00000028486 RLG00000028487 RLG00000028488 RLG00000028489 RLG00000028493 RLG00000028494 RLG00000035411
rosa_multiflora Rmu_co8173820.1_g000001 Rmu_sc0001037.1_g000006 Rmu_sc0001864.1_g000002 Rmu_sc0001864.1_g000009 Rmu_ssc0000083.1_g000008 Rmu_ssc0000083.1_g000013 Rmu_ssc0000083.1_g000018
rosa_roxburghii Rroxscaffold_4G00304840 Rroxscaffold_4G00304860 Rroxscaffold_4G00304890 Rroxscaffold_4G00304900 Rroxscaffold_4G00304920 Rroxscaffold_4G00304930
rosa_rugosa Rorug01G0210800 Rorug01G0210900 Rorug01G0211000 Rorug01G0211100 Rorug01G0211200 Rorug01G0211300 Rorug01G0211300 Rorug01G0211400
rosa_samantha Rh1AG225500 Rh1AG225600 Rh1AG225700 Rh1AG225800 Rh1AG225900 Rh1AG226000 Rh1BG193400 Rh1BG193600 Rh1BG193700 Rh1BG193800 Rh1BG193900 Rh1BG194000 Rh1BG194200 Rh1CG210300 Rh1CG210500 Rh1CG210600 Rh1CG210700 Rh1CG210900 Rh1DG221700 Rh1DG221900 Rh1DG222100 Rh1DG222200 Rh1DG222300 Rh1DG222400 Rh1DG222500 Rh2BG259100 Rh3DG280100 Rh5AG528000
rosa_wichuraiana Rw0G018730 Rw0G018740 Rw0G018750 Rw0G018760 Rw0G018770 Rw0G018790 Rw1G019390 Rw1G019410 Rw1G019420 Rw1G019430 Rw1G019440 Rw1G019450 Rw1G019480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 203
AccB1I GGYRCC 2 cut(s) 701, 875
AccBSI CCGCTC 1 cut(s) 168
AccI GTMKAC 1 cut(s) 42
AclWI GGATC 1 cut(s) 771
AcoI YGGCCR 1 cut(s) 121
AcuI CTGAAG 1 cut(s) 429
AcyI GRCGYC 1 cut(s) 339
AfaI GTAC 4 cut(s) 139, 186, 477, 1103
AfiI CCNNNNNNNGG 2 cut(s) 779, 930
AgsI TTSAA 1 cut(s) 376
AhlI ACTAGT 1 cut(s) 34
AjiI CACGTC 1 cut(s) 145
AjnI CCWGG 1 cut(s) 603
AluBI AGCT 4 cut(s) 73, 319, 670, 936
AluI AGCT 4 cut(s) 73, 319, 670, 936
Alw21I GWGCWC 1 cut(s) 321
Alw26I GTCTC 1 cut(s) 848
AlwI GGATC 1 cut(s) 771
Ama87I CYCGRG 1 cut(s) 307
AoxI GGCC 3 cut(s) 22, 121, 647
ApeKI GCWGC 6 cut(s) 73, 117, 126, 635, 920, 1083
AspS9I GGNCC 1 cut(s) 648
AsuC2I CCSGG 2 cut(s) 156, 492
AsuHPI GGTGA 6 cut(s) 231, 292, 358, 413, 506, 563
AsuII TTCGAA 2 cut(s) 597, 964
AvaI CYCGRG 1 cut(s) 307
BanI GGYRCC 2 cut(s) 701, 875
BanII GRGCYC 1 cut(s) 321
Bbv12I GWGCWC 1 cut(s) 321
BbvI GCAGC 6 cut(s) 60, 104, 113, 647, 932, 1070
BccI CCATC 3 cut(s) 557, 784, 846
BceAI ACGGC 3 cut(s) 167, 548, 1001
BciT130I CCWGG 1 cut(s) 605
BcnI CCSGG 2 cut(s) 156, 492
BcoDI GTCTC 1 cut(s) 848
BcuI ACTAGT 1 cut(s) 34
BfaI CTAG 1 cut(s) 35
BisI GCNGC 8 cut(s) 74, 118, 121, 124, 127, 636, 921, 1084
BlsI GCNGC 8 cut(s) 75, 119, 122, 125, 128, 637, 922, 1085
Bme1390I CCNGG 3 cut(s) 156, 492, 605
BmeT110I CYCGRG 1 cut(s) 307
BmgBI CACGTC 1 cut(s) 145
BmgT120I GGNCC 1 cut(s) 648
BmiI GGNNCC 2 cut(s) 703, 877
BmrFI CCNGG 3 cut(s) 156, 492, 605
BmsI GCATC 1 cut(s) 339
Bpu14I TTCGAA 2 cut(s) 597, 964
BpuMI CCSGG 2 cut(s) 156, 492
BsaBI GATNNNNATC 2 cut(s) 95, 837
BsaHI GRCGYC 1 cut(s) 339
BsaJI CCNNGG 2 cut(s) 603, 604
Bsc4I CCNNNNNNNGG 2 cut(s) 779, 930
Bse1I ACTGG 3 cut(s) 135, 714, 1084
Bse8I GATNNNNATC 2 cut(s) 95, 837
BseBI CCWGG 1 cut(s) 605
BseDI CCNNGG 2 cut(s) 603, 604
BseGI GGATG 4 cut(s) 107, 319, 488, 894
BseJI GATNNNNATC 2 cut(s) 95, 837
BseLI CCNNNNNNNGG 2 cut(s) 779, 930
BseNI ACTGG 3 cut(s) 135, 714, 1084
BseRI GAGGAG 1 cut(s) 350
BseX3I CGGCCG 1 cut(s) 121
BseXI GCAGC 6 cut(s) 60, 104, 113, 647, 932, 1070
Bsh1285I CGRYCG 1 cut(s) 124
BshFI GGCC 3 cut(s) 24, 123, 649
BshNI GGYRCC 2 cut(s) 701, 875
BsiEI CGRYCG 1 cut(s) 124
BsiHKAI GWGCWC 1 cut(s) 321
BsiHKCI CYCGRG 1 cut(s) 307
BsiSI CCGG 2 cut(s) 155, 491
BslI CCNNNNNNNGG 2 cut(s) 779, 930
BsmAI GTCTC 1 cut(s) 848
BsnI GGCC 3 cut(s) 24, 123, 649
BsoBI CYCGRG 1 cut(s) 307
Bsp119I TTCGAA 2 cut(s) 597, 964
Bsp1286I GDGCHC 1 cut(s) 321
Bsp1407I TGTACA 2 cut(s) 475, 1101
Bsp143I GATC 2 cut(s) 282, 763
BspANI GGCC 3 cut(s) 24, 123, 649
BspLI GGNNCC 2 cut(s) 703, 877
BspPI GGATC 1 cut(s) 771
BspT104I TTCGAA 2 cut(s) 597, 964
BspT107I GGYRCC 2 cut(s) 701, 875
BsrBI CCGCTC 1 cut(s) 168
BsrGI TGTACA 2 cut(s) 475, 1101
BsrI ACTGG 3 cut(s) 135, 714, 1084
BssECI CCNNGG 2 cut(s) 603, 604
BssMI GATC 2 cut(s) 282, 763
BssNI GRCGYC 1 cut(s) 339
Bst2UI CCWGG 1 cut(s) 605
Bst4CI ACNGT 1 cut(s) 47
BstACI GRCGYC 1 cut(s) 339
BstAUI TGTACA 2 cut(s) 475, 1101
BstBI TTCGAA 2 cut(s) 597, 964
BstC8I GCNNGC 3 cut(s) 223, 350, 1088
BstDEI CTNAG 1 cut(s) 1058
BstENI CCTNNNNNAGG 1 cut(s) 777
BstF5I GGATG 4 cut(s) 107, 319, 488, 894
BstKTI GATC 2 cut(s) 285, 766
BstMAI GTCTC 1 cut(s) 848
BstMBI GATC 2 cut(s) 282, 763
BstMCI CGRYCG 1 cut(s) 124
BstMWI GCNNNNNNNGC 4 cut(s) 123, 126, 165, 259
BstNI CCWGG 1 cut(s) 605
BstNSI RCATGY 2 cut(s) 221, 352
BstSCI CCNGG 3 cut(s) 154, 490, 603
BstV1I GCAGC 6 cut(s) 60, 104, 113, 647, 932, 1070
BstZI CGGCCG 1 cut(s) 121
BsuRI GGCC 3 cut(s) 24, 123, 649
BtgZI GCGATG 1 cut(s) 752
BtrI CACGTC 1 cut(s) 145
BtsCI GGATG 4 cut(s) 107, 319, 488, 894
BtsIMutI CAGTG 1 cut(s) 405
Cac8I GCNNGC 3 cut(s) 223, 350, 1088
CciNI GCGGCCGC 1 cut(s) 121
Cfr13I GGNCC 1 cut(s) 648
CseI GACGC 1 cut(s) 347
Csp6I GTAC 4 cut(s) 138, 185, 476, 1102
CviQI GTAC 4 cut(s) 138, 185, 476, 1102
DdeI CTNAG 1 cut(s) 1058
DpnI GATC 2 cut(s) 284, 765
DpnII GATC 2 cut(s) 282, 763
EaeI YGGCCR 1 cut(s) 121
EagI CGGCCG 1 cut(s) 121
EciI GGCGGA 3 cut(s) 240, 283, 1050
Ecl136II GAGCTC 1 cut(s) 319
EclXI CGGCCG 1 cut(s) 121
Eco24I GRGCYC 1 cut(s) 321
Eco32I GATATC 1 cut(s) 294
Eco52I CGGCCG 1 cut(s) 121
Eco53kI GAGCTC 1 cut(s) 319
Eco57I CTGAAG 1 cut(s) 429
Eco88I CYCGRG 1 cut(s) 307
EcoICRI GAGCTC 1 cut(s) 319
EcoNI CCTNNNNNAGG 1 cut(s) 777
EcoRII CCWGG 1 cut(s) 603
EcoRV GATATC 1 cut(s) 294
EcoT22I ATGCAT 1 cut(s) 350
EcoT38I GRGCYC 1 cut(s) 321
FauI CCCGC 2 cut(s) 173, 281
FauNDI CATATG 1 cut(s) 928
FblI GTMKAC 1 cut(s) 42
Fnu4HI GCNGC 8 cut(s) 74, 118, 121, 124, 127, 636, 921, 1084
FokI GGATG 4 cut(s) 94, 326, 495, 881
FriOI GRGCYC 1 cut(s) 321
Fsp4HI GCNGC 8 cut(s) 74, 118, 121, 124, 127, 636, 921, 1084
FspBI CTAG 1 cut(s) 35
GluI GCNGC 8 cut(s) 74, 118, 121, 124, 127, 636, 921, 1084
HaeIII GGCC 3 cut(s) 24, 123, 649
HapII CCGG 2 cut(s) 155, 491
HgaI GACGC 1 cut(s) 347
Hin1I GRCGYC 1 cut(s) 339
HincII GTYRAC 2 cut(s) 43, 488
HindII GTYRAC 2 cut(s) 43, 488
HindIII AAGCTT 1 cut(s) 668
HinfI GANTC 5 cut(s) 85, 389, 433, 497, 686
HpaII CCGG 2 cut(s) 155, 491
HphI GGTGA 6 cut(s) 231, 292, 358, 413, 506, 563
Hpy166II GTNNAC 6 cut(s) 43, 488, 530, 806, 1102, 1104
Hpy188I TCNGA 1 cut(s) 90
Hpy188III TCNNGA 1 cut(s) 383
Hpy8I GTNNAC 6 cut(s) 43, 488, 530, 806, 1102, 1104
Hpy99I CGWCG 2 cut(s) 104, 149
HpyAV CCTTC 4 cut(s) 87, 268, 450, 512
HpyCH4III ACNGT 1 cut(s) 47
HpyCH4IV ACGT 2 cut(s) 144, 258
HpyCH4V TGCA 6 cut(s) 262, 330, 348, 635, 677, 729
HpyF10VI GCNNNNNNNGC 4 cut(s) 123, 126, 165, 259
HpyF3I CTNAG 1 cut(s) 1058
HpySE526I ACGT 2 cut(s) 144, 258
Hsp92I GRCGYC 1 cut(s) 339
Kzo9I GATC 2 cut(s) 282, 763
LmnI GCTCC 2 cut(s) 173, 933
Lsp1109I GCAGC 6 cut(s) 60, 104, 113, 647, 932, 1070
LweI GCATC 1 cut(s) 339
MaeI CTAG 1 cut(s) 35
MaeII ACGT 2 cut(s) 144, 258
MaeIII GTNAC 2 cut(s) 494, 1003
MalI GATC 2 cut(s) 284, 765
MbiI CCGCTC 1 cut(s) 168
MboI GATC 2 cut(s) 282, 763
MboII GAAGA 4 cut(s) 283, 398, 422, 425
MfeI CAATTG 2 cut(s) 322, 639
MhlI GDGCHC 1 cut(s) 321
MluCI AATT 6 cut(s) 13, 322, 541, 639, 678, 1073
MlyI GAGTC 2 cut(s) 94, 491
Mph1103I ATGCAT 1 cut(s) 350
MseI TTAA 1 cut(s) 1076
MslI CAYNNNNRTG 3 cut(s) 398, 552, 947
MspA1I CMGCKG 4 cut(s) 73, 117, 126, 825
MspI CCGG 2 cut(s) 155, 491
MspR9I CCNGG 3 cut(s) 156, 492, 605
MunI CAATTG 2 cut(s) 322, 639
MvaI CCWGG 1 cut(s) 605
MwoI GCNNNNNNNGC 4 cut(s) 123, 126, 165, 259
NciI CCSGG 2 cut(s) 156, 492
NdeI CATATG 1 cut(s) 928
NdeII GATC 2 cut(s) 282, 763
NlaIV GGNNCC 2 cut(s) 703, 877
NmuCI GTSAC 1 cut(s) 494
NotI GCGGCCGC 1 cut(s) 121
NsiI ATGCAT 1 cut(s) 350
NspI RCATGY 2 cut(s) 221, 352
NspV TTCGAA 2 cut(s) 597, 964
PaeI GCATGC 1 cut(s) 352
PaeR7I CTCGAG 1 cut(s) 307
PasI CCCWGGG 1 cut(s) 604
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PfeI GAWTC 3 cut(s) 389, 433, 686
PkrI GCNGC 8 cut(s) 75, 119, 122, 125, 128, 637, 922, 1085
PleI GAGTC 2 cut(s) 93, 491
PpsI GAGTC 2 cut(s) 93, 491
PsiI TTATAA 1 cut(s) 203
Psp124BI GAGCTC 1 cut(s) 321
Psp6I CCWGG 1 cut(s) 603
PspGI CCWGG 1 cut(s) 603
PspN4I GGNNCC 2 cut(s) 703, 877
PspPI GGNCC 1 cut(s) 648
PspXI VCTCGAGB 1 cut(s) 307
PvuII CAGCTG 1 cut(s) 73
RsaI GTAC 4 cut(s) 139, 186, 477, 1103
RsaNI GTAC 4 cut(s) 138, 185, 476, 1102
RseI CAYNNNNRTG 3 cut(s) 398, 552, 947
SacI GAGCTC 1 cut(s) 321
SalI GTCGAC 1 cut(s) 41
SaqAI TTAA 1 cut(s) 1076
SatI GCNGC 8 cut(s) 74, 118, 121, 124, 127, 636, 921, 1084
Sau3AI GATC 2 cut(s) 282, 763
Sau96I GGNCC 1 cut(s) 648
SchI GAGTC 2 cut(s) 94, 491
ScrFI CCNGG 3 cut(s) 156, 492, 605
SduI GDGCHC 1 cut(s) 321
SfaNI GCATC 1 cut(s) 339
Sfr274I CTCGAG 1 cut(s) 307
SfuI TTCGAA 2 cut(s) 597, 964
SlaI CTCGAG 1 cut(s) 307
SmiMI CAYNNNNRTG 3 cut(s) 398, 552, 947
SmlI CTYRAG 1 cut(s) 307
SmoI CTYRAG 1 cut(s) 307
SpeI ACTAGT 1 cut(s) 34
SphI GCATGC 1 cut(s) 352
Sse9I AATT 6 cut(s) 13, 322, 541, 639, 678, 1073
SspMI CTAG 1 cut(s) 35
SstI GAGCTC 1 cut(s) 321
StyD4I CCNGG 3 cut(s) 154, 490, 603
TaaI ACNGT 1 cut(s) 47
TaiI ACGT 2 cut(s) 147, 261
TaqI TCGA 6 cut(s) 42, 308, 597, 906, 964, 1095
TasI AATT 6 cut(s) 13, 322, 541, 639, 678, 1073
TatI WGTACW 2 cut(s) 475, 1101
TauI GCSGC 2 cut(s) 123, 126
TfiI GAWTC 3 cut(s) 389, 433, 686
Tru1I TTAA 1 cut(s) 1076
Tru9I TTAA 1 cut(s) 1076
TscAI CASTG 1 cut(s) 412
TseFI GTSAC 1 cut(s) 494
TseI GCWGC 6 cut(s) 73, 117, 126, 635, 920, 1083
Tsp45I GTSAC 1 cut(s) 494
TspDTI ATGAA 4 cut(s) 570, 588, 662, 883
TspGWI ACGGA 1 cut(s) 100
TspRI CASTG 1 cut(s) 412
XagI CCTNNNNNAGG 1 cut(s) 777
XceI RCATGY 2 cut(s) 221, 352
XcmI CCANNNNNNNNNTGG 2 cut(s) 783, 967
XhoI CTCGAG 1 cut(s) 307
XmiI GTMKAC 1 cut(s) 42
XspI CTAG 1 cut(s) 35
Zsp2I ATGCAT 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.