MD08G1231300.v1.1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000382
Physical Location & Seq
Reverse (-)
29750025 .. 29751254
1230 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1231300.v1.1.491

Sequence Viewer

Length: 804 bp
ATGCCAGAGAACCAGAGAGATCTAAGCTACCTCACAAGTCTACCAGGAGCAACCGAAAGGCTCAAAATTTTCAACGCGGATCTCAGCAAACCCGACAGTTTCAATGCAGCCGTCGAAGGGTGCATCGGAGTGTTCCATGTTGCCACTCCAGTCGATTTCCAAGACAAGGAACCTGAAGAAGTAGTGACCAAAAGATCAATTGATGGAGCCCTCGGCATTCTGAAGGCGTGCTTAAACGCAAAAACAGTGAAACGAGTTGTGTACACTTCTAGTGCAGCCGCTGTCATGTTTGGCAGCAGCAAGGATGTTGAGGAAGTGGATGAAAGTTTTTGGAGCGATATAGATTACATTAGACCTATAAAGCGATATGGACCATACATGATTTCGAAGACATTAACTGAGAAGCCAGTTCTTGAATTTTCAGAGAAATATGGGTTGGATGCTGTGACAGTAATACCTTCTTTTGTGGTTGGCCACTTCATTTGTCCTAAGCTTCCCGGCTCCATTCACGGTACACTGACTCTGGTCTTTGCGCACATTTTCTTTTTCGAACATCATGATGCAAAAGGGAGGTGCAATTGCTCATCACATGTCATAACCCTTGTTCAAACGGCTGAGTTTCTTTCTGCCAAATACCCGGAGTTTCAAATACCCTCAGTAAGGTATTTAAGGGAGCTTAAGGGTGAGAAAACACCTGGTATGTCGTCGAAGAAGCTCTTGGATTCAGGTTTCACATTCAAGTATGGGGTTGATGAAACGCTCATCGGTGCAATTCAATGCTGCAAAGAAAAGCATTATTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00943 ko01110 map00943 map01110
Pfam Domains
Protein Families
Epimerase

Protein Analysis

268

Amino Acids

29.73

Weight (kDa)

6.45

Isoelectric Point (pI)

33.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_4 PF07993 17 - 174 5.4e-08 Male sterility protein
Epimerase PF01370 18 - 160 6.7e-12 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 22 - 139 2.5e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45400 AT2G45400 AT2G45400 AT2G45400
fragaria_vesca FvH4_7g11750 FvH4_7g11760 FvH4_7g11780 FvH4_7g11781 FvH4_7g11781 FvH4_7g11790 FvH4_7g11790
malus_domestica MD02G1205000.v1.1 MD07G1121700.v1.1 MD08G1231300.v1.1 MD08G1231400.v1.1 MD08G1231500.v1.1
prunus_persica Prupe.2G144600_v2.0.a1 Prupe.2G144700_v2.0.a1 Prupe.2G144800_v2.0.a1
pyrus_communis pycom08g20120
rosa_chinensis RchiOBHm_Chr1g0350271 RchiOBHm_Chr1g0350281 RchiOBHm_Chr1g0350311 RchiOBHm_Chr1g0350321 RchiOBHm_Chr1g0350341 RchiOBHm_Chr1g0350361 RchiOBHm_Chr1g0350371 RchiOBHm_Chr1g0350401 RchiOBHm_Chr1g0350411 RchiOBHm_Chr1g0350431 RchiOBHm_Chr1g0350441 RchiOBHm_Chr1g0350471
rosa_laevigata RLG00000028483 RLG00000028485 RLG00000028486 RLG00000028487 RLG00000028488 RLG00000028489 RLG00000028493 RLG00000028494 RLG00000035411
rosa_multiflora Rmu_co8173820.1_g000001 Rmu_sc0001037.1_g000006 Rmu_sc0001864.1_g000002 Rmu_sc0001864.1_g000009 Rmu_ssc0000083.1_g000008 Rmu_ssc0000083.1_g000013 Rmu_ssc0000083.1_g000018
rosa_roxburghii Rroxscaffold_4G00304840 Rroxscaffold_4G00304860 Rroxscaffold_4G00304890 Rroxscaffold_4G00304900 Rroxscaffold_4G00304920 Rroxscaffold_4G00304930
rosa_rugosa Rorug01G0210800 Rorug01G0210900 Rorug01G0211000 Rorug01G0211100 Rorug01G0211200 Rorug01G0211300 Rorug01G0211300 Rorug01G0211400
rosa_samantha Rh1AG225500 Rh1AG225600 Rh1AG225700 Rh1AG225800 Rh1AG225900 Rh1AG226000 Rh1BG193400 Rh1BG193600 Rh1BG193700 Rh1BG193800 Rh1BG193900 Rh1BG194000 Rh1BG194200 Rh1CG210300 Rh1CG210500 Rh1CG210600 Rh1CG210700 Rh1CG210900 Rh1DG221700 Rh1DG221900 Rh1DG222100 Rh1DG222200 Rh1DG222300 Rh1DG222400 Rh1DG222500 Rh2BG259100 Rh3DG280100 Rh5AG528000
rosa_wichuraiana Rw0G018730 Rw0G018740 Rw0G018750 Rw0G018760 Rw0G018770 Rw0G018790 Rw1G019390 Rw1G019410 Rw1G019420 Rw1G019430 Rw1G019440 Rw1G019450 Rw1G019480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 534
AccI GTMKAC 1 cut(s) 40
AccII CGCG 1 cut(s) 77
AciI CCGC 2 cut(s) 77, 279
AclWI GGATC 1 cut(s) 87
AcoI YGGCCR 1 cut(s) 472
AcsI RAATTY 2 cut(s) 66, 416
AcuI CTGAAG 2 cut(s) 195, 242
AfaI GTAC 2 cut(s) 263, 514
AfiI CCNNNNNNNGG 3 cut(s) 117, 166, 660
AflII CTTAAG 1 cut(s) 677
AflIII ACRYGT 1 cut(s) 589
AgsI TTSAA 7 cut(s) 73, 103, 416, 608, 647, 739, 776
AjnI CCWGG 2 cut(s) 43, 694
AjuI GAANNNNNNNTTGG 4 cut(s) 419, 451, 701, 733
AluBI AGCT 4 cut(s) 27, 493, 676, 715
AluI AGCT 4 cut(s) 27, 493, 676, 715
AlwI GGATC 1 cut(s) 87
AlwNI CAGNNNCTG 1 cut(s) 281
AoxI GGCC 1 cut(s) 472
ApeKI GCWGC 5 cut(s) 107, 275, 294, 297, 780
ApoI RAATTY 2 cut(s) 66, 416
AspLEI GCGC 1 cut(s) 535
AspS9I GGNCC 1 cut(s) 371
AsuC2I CCSGG 2 cut(s) 498, 638
AsuHPI GGTGA 1 cut(s) 695
AsuII TTCGAA 2 cut(s) 386, 549
AvaII GGWCC 1 cut(s) 371
BalI TGGCCA 1 cut(s) 474
BanII GRGCYC 1 cut(s) 211
BbsI GAAGAC 1 cut(s) 395
BbvI GCAGC 5 cut(s) 119, 287, 306, 309, 767
BccI CCATC 1 cut(s) 197
BceAI ACGGC 2 cut(s) 95, 627
BciT130I CCWGG 2 cut(s) 45, 696
BcnI CCSGG 2 cut(s) 498, 638
BfaI CTAG 1 cut(s) 270
BfrI CTTAAG 1 cut(s) 677
BglII AGATCT 1 cut(s) 19
BisI GCNGC 6 cut(s) 108, 276, 279, 295, 298, 781
BlsI GCNGC 6 cut(s) 109, 277, 280, 296, 299, 782
Bme1390I CCNGG 4 cut(s) 45, 498, 638, 696
Bme18I GGWCC 1 cut(s) 371
BmgT120I GGNCC 1 cut(s) 371
BmiI GGNNCC 3 cut(s) 171, 208, 502
BmrFI CCNGG 4 cut(s) 45, 498, 638, 696
BmsI GCATC 3 cut(s) 132, 430, 550
BoxI GACNNNNGTC 1 cut(s) 524
BpiI GAAGAC 1 cut(s) 395
BpmI CTGGAG 1 cut(s) 132
Bpu10I CCTNAGC 1 cut(s) 489
Bpu14I TTCGAA 2 cut(s) 386, 549
BpuMI CCSGG 2 cut(s) 498, 638
BsaJI CCNNGG 1 cut(s) 211
Bsc4I CCNNNNNNNGG 3 cut(s) 117, 166, 660
Bse1I ACTGG 2 cut(s) 149, 407
BseBI CCWGG 2 cut(s) 45, 696
BseDI CCNNGG 1 cut(s) 211
BseGI GGATG 3 cut(s) 310, 325, 445
BseLI CCNNNNNNNGG 3 cut(s) 117, 166, 660
BseMII CTCAG 4 cut(s) 97, 390, 606, 669
BseNI ACTGG 2 cut(s) 149, 407
BseXI GCAGC 5 cut(s) 119, 287, 306, 309, 767
BsgI GTGCAG 1 cut(s) 294
Bsh1236I CGCG 1 cut(s) 77
BshFI GGCC 1 cut(s) 474
BsiSI CCGG 2 cut(s) 498, 638
BslI CCNNNNNNNGG 3 cut(s) 117, 166, 660
BsmI GAATGC 1 cut(s) 216
BsnI GGCC 1 cut(s) 474
Bsp119I TTCGAA 2 cut(s) 386, 549
Bsp1286I GDGCHC 1 cut(s) 211
Bsp1407I TGTACA 1 cut(s) 261
Bsp143I GATC 3 cut(s) 19, 79, 194
BspACI CCGC 2 cut(s) 77, 279
BspANI GGCC 1 cut(s) 474
BspCNI CTCAG 4 cut(s) 96, 391, 607, 668
BspFNI CGCG 1 cut(s) 77
BspHI TCATGA 1 cut(s) 556
BspLI GGNNCC 3 cut(s) 171, 208, 502
BspPI GGATC 1 cut(s) 87
BspT104I TTCGAA 2 cut(s) 386, 549
BspTI CTTAAG 1 cut(s) 677
BsrGI TGTACA 1 cut(s) 261
BsrI ACTGG 2 cut(s) 149, 407
BssECI CCNNGG 1 cut(s) 211
BssMI GATC 3 cut(s) 19, 79, 194
Bst2UI CCWGG 2 cut(s) 45, 696
Bst4CI ACNGT 4 cut(s) 98, 247, 451, 512
BstAFI CTTAAG 1 cut(s) 677
BstAUI TGTACA 1 cut(s) 261
BstBI TTCGAA 2 cut(s) 386, 549
BstC8I GCNNGC 1 cut(s) 229
BstDEI CTNAG 6 cut(s) 23, 83, 399, 489, 615, 655
BstENI CCTNNNNNAGG 1 cut(s) 658
BstF5I GGATG 3 cut(s) 310, 325, 445
BstFNI CGCG 1 cut(s) 77
BstHHI GCGC 1 cut(s) 535
BstKTI GATC 3 cut(s) 22, 82, 197
BstMBI GATC 3 cut(s) 19, 79, 194
BstNI CCWGG 2 cut(s) 45, 696
BstNSI RCATGY 1 cut(s) 593
BstPAI GACNNNNGTC 1 cut(s) 524
BstSCI CCNGG 4 cut(s) 43, 496, 636, 694
BstUI CGCG 1 cut(s) 77
BstV1I GCAGC 5 cut(s) 119, 287, 306, 309, 767
BstV2I GAAGAC 1 cut(s) 395
BstX2I RGATCY 2 cut(s) 19, 79
BstYI RGATCY 2 cut(s) 19, 79
BsuRI GGCC 1 cut(s) 474
BtsCI GGATG 3 cut(s) 310, 325, 445
BtsIMutI CAGTG 2 cut(s) 252, 515
Cac8I GCNNGC 1 cut(s) 229
CaiI CAGNNNCTG 1 cut(s) 281
CciI TCATGA 1 cut(s) 556
CfoI GCGC 1 cut(s) 535
Cfr13I GGNCC 1 cut(s) 371
CsiI ACCWGGT 1 cut(s) 694
Csp6I GTAC 2 cut(s) 262, 513
CspCI CAANNNNNGTGG 2 cut(s) 464, 499
CviAII CATG 5 cut(s) 137, 286, 379, 557, 590
CviQI GTAC 2 cut(s) 262, 513
DdeI CTNAG 6 cut(s) 23, 83, 399, 489, 615, 655
DpnI GATC 3 cut(s) 21, 81, 196
DpnII GATC 3 cut(s) 19, 79, 194
EaeI YGGCCR 1 cut(s) 472
Eco24I GRGCYC 1 cut(s) 211
Eco47I GGWCC 1 cut(s) 371
Eco57I CTGAAG 2 cut(s) 195, 242
EcoNI CCTNNNNNAGG 1 cut(s) 658
EcoRII CCWGG 2 cut(s) 43, 694
EcoT38I GRGCYC 1 cut(s) 211
FaeI CATG 5 cut(s) 140, 289, 382, 560, 593
FalI AAGNNNNNCTT 4 cut(s) 215, 247, 701, 733
FatI CATG 5 cut(s) 136, 285, 378, 556, 589
FblI GTMKAC 1 cut(s) 40
Fnu4HI GCNGC 6 cut(s) 108, 276, 279, 295, 298, 781
FokI GGATG 3 cut(s) 317, 332, 452
FriOI GRGCYC 1 cut(s) 211
Fsp4HI GCNGC 6 cut(s) 108, 276, 279, 295, 298, 781
FspBI CTAG 1 cut(s) 270
FspI TGCGCA 1 cut(s) 534
GlaI GCGC 1 cut(s) 534
GluI GCNGC 6 cut(s) 108, 276, 279, 295, 298, 781
GsuI CTGGAG 1 cut(s) 132
HaeIII GGCC 1 cut(s) 474
HapII CCGG 2 cut(s) 498, 638
HhaI GCGC 1 cut(s) 535
Hin1II CATG 5 cut(s) 140, 289, 382, 560, 593
Hin6I GCGC 1 cut(s) 533
HinP1I GCGC 1 cut(s) 533
HindIII AAGCTT 1 cut(s) 491
HinfI GANTC 2 cut(s) 520, 722
HpaII CCGG 2 cut(s) 498, 638
HphI GGTGA 1 cut(s) 695
Hpy166II GTNNAC 4 cut(s) 41, 262, 264, 515
Hpy188I TCNGA 3 cut(s) 128, 222, 424
Hpy188III TCNNGA 2 cut(s) 413, 557
Hpy8I GTNNAC 4 cut(s) 41, 262, 264, 515
Hpy99I CGWCG 2 cut(s) 116, 709
HpyAV CCTTC 3 cut(s) 110, 217, 468
HpyCH4III ACNGT 4 cut(s) 98, 247, 451, 512
HpyCH4V TGCA 7 cut(s) 107, 123, 275, 563, 576, 770, 783
HpyF3I CTNAG 6 cut(s) 23, 83, 399, 489, 615, 655
Hsp92II CATG 5 cut(s) 140, 289, 382, 560, 593
HspAI GCGC 1 cut(s) 533
Kzo9I GATC 3 cut(s) 19, 79, 194
LmnI GCTCC 5 cut(s) 47, 206, 333, 506, 673
Lsp1109I GCAGC 5 cut(s) 119, 287, 306, 309, 767
LweI GCATC 3 cut(s) 132, 430, 550
MabI ACCWGGT 1 cut(s) 694
MaeI CTAG 1 cut(s) 270
MaeIII GTNAC 2 cut(s) 184, 445
MalI GATC 3 cut(s) 21, 81, 196
MboI GATC 3 cut(s) 19, 79, 194
MboII GAAGA 3 cut(s) 188, 400, 721
MfeI CAATTG 2 cut(s) 198, 577
MflI RGATCY 2 cut(s) 19, 79
MhlI GDGCHC 1 cut(s) 211
MlsI TGGCCA 1 cut(s) 474
MluCI AATT 5 cut(s) 66, 198, 416, 577, 771
MluNI TGGCCA 1 cut(s) 474
MlyI GAGTC 1 cut(s) 514
MmeI TCCRAC 1 cut(s) 417
MnlI CCTC 5 cut(s) 41, 221, 304, 564, 664
Mox20I TGGCCA 1 cut(s) 474
MscI TGGCCA 1 cut(s) 474
MseI TTAA 4 cut(s) 233, 395, 668, 678
MslI CAYNNNNRTG 2 cut(s) 128, 558
Msp20I TGGCCA 1 cut(s) 474
MspA1I CMGCKG 1 cut(s) 281
MspCI CTTAAG 1 cut(s) 677
MspI CCGG 2 cut(s) 498, 638
MspR9I CCNGG 4 cut(s) 45, 498, 638, 696
MunI CAATTG 2 cut(s) 198, 577
Mva1269I GAATGC 1 cut(s) 216
MvaI CCWGG 2 cut(s) 45, 696
MvnI CGCG 1 cut(s) 77
NciI CCSGG 2 cut(s) 498, 638
NdeII GATC 3 cut(s) 19, 79, 194
NlaIII CATG 5 cut(s) 140, 289, 382, 560, 593
NlaIV GGNNCC 3 cut(s) 171, 208, 502
NmeAIII GCCGAG 1 cut(s) 192
NmuCI GTSAC 2 cut(s) 184, 445
NsbI TGCGCA 1 cut(s) 534
NspI RCATGY 1 cut(s) 593
NspV TTCGAA 2 cut(s) 386, 549
PagI TCATGA 1 cut(s) 556
PciI ACATGT 1 cut(s) 589
PctI GAATGC 1 cut(s) 216
PfeI GAWTC 1 cut(s) 722
PkrI GCNGC 6 cut(s) 109, 277, 280, 296, 299, 782
PleI GAGTC 1 cut(s) 514
PpsI GAGTC 1 cut(s) 514
PscI ACATGT 1 cut(s) 589
PshAI GACNNNNGTC 1 cut(s) 524
Psp6I CCWGG 2 cut(s) 43, 694
PspGI CCWGG 2 cut(s) 43, 694
PspN4I GGNNCC 3 cut(s) 171, 208, 502
PspPI GGNCC 1 cut(s) 371
PstNI CAGNNNCTG 1 cut(s) 281
PsuI RGATCY 2 cut(s) 19, 79
RsaI GTAC 2 cut(s) 263, 514
RsaNI GTAC 2 cut(s) 262, 513
RseI CAYNNNNRTG 2 cut(s) 128, 558
SaqAI TTAA 4 cut(s) 233, 395, 668, 678
SatI GCNGC 6 cut(s) 108, 276, 279, 295, 298, 781
Sau3AI GATC 3 cut(s) 19, 79, 194
Sau96I GGNCC 1 cut(s) 371
SchI GAGTC 1 cut(s) 514
ScrFI CCNGG 4 cut(s) 45, 498, 638, 696
SduI GDGCHC 1 cut(s) 211
SexAI ACCWGGT 1 cut(s) 694
SfaNI GCATC 3 cut(s) 132, 430, 550
SfuI TTCGAA 2 cut(s) 386, 549
SinI GGWCC 1 cut(s) 371
SmiMI CAYNNNNRTG 2 cut(s) 128, 558
SmlI CTYRAG 1 cut(s) 677
SmoI CTYRAG 1 cut(s) 677
Sse9I AATT 5 cut(s) 66, 198, 416, 577, 771
SsiI CCGC 2 cut(s) 77, 279
SspMI CTAG 1 cut(s) 270
StyD4I CCNGG 4 cut(s) 43, 496, 636, 694
TaaI ACNGT 4 cut(s) 98, 247, 451, 512
TaqI TCGA 5 cut(s) 114, 153, 386, 549, 707
TasI AATT 5 cut(s) 66, 198, 416, 577, 771
TatI WGTACW 1 cut(s) 261
TauI GCSGC 1 cut(s) 281
TfiI GAWTC 1 cut(s) 722
Tru1I TTAA 4 cut(s) 233, 395, 668, 678
Tru9I TTAA 4 cut(s) 233, 395, 668, 678
TscAI CASTG 2 cut(s) 252, 522
TseFI GTSAC 2 cut(s) 184, 445
TseI GCWGC 5 cut(s) 107, 275, 294, 297, 780
Tsp45I GTSAC 2 cut(s) 184, 445
TspDTI ATGAA 3 cut(s) 336, 469, 768
TspRI CASTG 2 cut(s) 252, 522
Vha464I CTTAAG 1 cut(s) 677
VpaK11BI GGWCC 1 cut(s) 371
XagI CCTNNNNNAGG 1 cut(s) 658
XapI RAATTY 2 cut(s) 66, 416
XceI RCATGY 1 cut(s) 593
XmiI GTMKAC 1 cut(s) 40
XspI CTAG 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.