Rh1AG225800

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000382
Physical Location & Seq
Forward (+)
42934279 .. 42950827
16549 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG225800.1

Sequence Viewer

Length: 753 bp
ATGGAAGGAGAGAGAGGTAGACTGTGTGTAACGGGAGGTTCAGGGTTCATAGGGTCCTGGTTGATTATGAGGCTTCTTGAGCATGGTTACTATGTCAATACCACTATTAGATCAGACCCAGAACACAAGAAAGATTTAAGCTTCCTCACAGCTCTACCAGGAGCAGAAGAAGAAAGGCTCCAAATATTCAACGCGGACCTAAGCAACCCGGAAAGTTTCAATGCAGCCATCCAAGGATGCGTCAGAGAGTTCCATGTTGCTACTCTGGTTGACTTCCAAGACAAGGAACCTGAACCAGTAGTGACCAAACGATCAGTCGATGGAGCCCTTGGTATCCTCAAGGCATGCCTATATGCAAAAACAATGAAACGAGTTGTGTACACTTCTAGTGAGTCTGCTGTTGCATTTAACAACAAGGATGTGGAAGACATGGATGAGAGTTTTTGGAGTGACACAGATTACATTAAAGCTTTAAAGTCGTATGGAGGCTCATACGCGATTTCGAAGACATTGACTGAAAGAGCAGTTCTTGAATTTTCGGAGAAAAATGGATTGGATGTTGTGACGCCTGTTGGAGTTGGCAAATTTGGAAATGCCTTGTTTTCAAGGATTAGTGAAGGATACTGCACATTTAAAATGACAGACATGAAAATGAAGGATCTAAAATCCAAATCTAGAATAATTAAGAATTTATTTGATTCCAAAGTCCTAGCTAGCCACACGTTAAAAAGCTTGTTTGGCATCTATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00943 ko01110 map00943 map01110
Pfam Domains
Protein Families
Epimerase

Protein Analysis

250

Amino Acids

27.83

Weight (kDa)

6.45

Isoelectric Point (pI)

34.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 9 - 189 1.5e-14 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 189 9.6e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 189 3e-11 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 188 1.5e-10 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45400 AT2G45400 AT2G45400 AT2G45400
fragaria_vesca FvH4_7g11750 FvH4_7g11760 FvH4_7g11780 FvH4_7g11781 FvH4_7g11781 FvH4_7g11790 FvH4_7g11790
malus_domestica MD02G1205000.v1.1 MD07G1121700.v1.1 MD08G1231300.v1.1 MD08G1231400.v1.1 MD08G1231500.v1.1
prunus_persica Prupe.2G144600_v2.0.a1 Prupe.2G144700_v2.0.a1 Prupe.2G144800_v2.0.a1
pyrus_communis pycom08g20120
rosa_chinensis RchiOBHm_Chr1g0350271 RchiOBHm_Chr1g0350281 RchiOBHm_Chr1g0350311 RchiOBHm_Chr1g0350321 RchiOBHm_Chr1g0350341 RchiOBHm_Chr1g0350361 RchiOBHm_Chr1g0350371 RchiOBHm_Chr1g0350401 RchiOBHm_Chr1g0350411 RchiOBHm_Chr1g0350431 RchiOBHm_Chr1g0350441 RchiOBHm_Chr1g0350471
rosa_laevigata RLG00000028483 RLG00000028485 RLG00000028486 RLG00000028487 RLG00000028488 RLG00000028489 RLG00000028493 RLG00000028494 RLG00000035411
rosa_multiflora Rmu_co8173820.1_g000001 Rmu_sc0001037.1_g000006 Rmu_sc0001864.1_g000002 Rmu_sc0001864.1_g000009 Rmu_ssc0000083.1_g000008 Rmu_ssc0000083.1_g000013 Rmu_ssc0000083.1_g000018
rosa_roxburghii Rroxscaffold_4G00304840 Rroxscaffold_4G00304860 Rroxscaffold_4G00304890 Rroxscaffold_4G00304900 Rroxscaffold_4G00304920 Rroxscaffold_4G00304930
rosa_rugosa Rorug01G0210800 Rorug01G0210900 Rorug01G0211000 Rorug01G0211100 Rorug01G0211200 Rorug01G0211300 Rorug01G0211300 Rorug01G0211400
rosa_samantha Rh1AG225500 Rh1AG225600 Rh1AG225700 Rh1AG225800 Rh1AG225900 Rh1AG226000 Rh1BG193400 Rh1BG193600 Rh1BG193700 Rh1BG193800 Rh1BG193900 Rh1BG194000 Rh1BG194200 Rh1CG210300 Rh1CG210500 Rh1CG210600 Rh1CG210700 Rh1CG210900 Rh1DG221700 Rh1DG221900 Rh1DG222100 Rh1DG222200 Rh1DG222300 Rh1DG222400 Rh1DG222500 Rh2BG259100 Rh3DG280100 Rh5AG528000
rosa_wichuraiana Rw0G018730 Rw0G018740 Rw0G018750 Rw0G018760 Rw0G018770 Rw0G018790 Rw1G019390 Rw1G019410 Rw1G019420 Rw1G019430 Rw1G019440 Rw1G019450 Rw1G019480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 19
AccII CGCG 2 cut(s) 194, 497
AciI CCGC 1 cut(s) 194
AclWI GGATC 1 cut(s) 666
AcsI RAATTY 3 cut(s) 533, 584, 688
AcyI GRCGYC 1 cut(s) 566
AfaI GTAC 1 cut(s) 380
AfiI CCNNNNNNNGG 1 cut(s) 283
AflIII ACRYGT 1 cut(s) 720
AgsI TTSAA 4 cut(s) 190, 220, 533, 606
AjnI CCWGG 2 cut(s) 56, 157
AjuI GAANNNNNNNTTGG 2 cut(s) 536, 568
AluBI AGCT 5 cut(s) 141, 152, 470, 713, 732
AluI AGCT 5 cut(s) 141, 152, 470, 713, 732
AlwI GGATC 1 cut(s) 666
ApeKI GCWGC 1 cut(s) 224
ApoI RAATTY 3 cut(s) 533, 584, 688
ArsI GACNNNNNNTTYG 2 cut(s) 300, 332
AspS9I GGNCC 2 cut(s) 54, 196
AsuC2I CCSGG 1 cut(s) 209
AsuII TTCGAA 1 cut(s) 503
AsuNHI GCTAGC 1 cut(s) 713
AvaII GGWCC 2 cut(s) 54, 196
BanII GRGCYC 1 cut(s) 328
BbsI GAAGAC 2 cut(s) 432, 512
BbvI GCAGC 1 cut(s) 236
BccI CCATC 2 cut(s) 236, 314
BciT130I CCWGG 2 cut(s) 58, 159
BciVI GTATCC 2 cut(s) 344, 614
BcnI CCSGG 1 cut(s) 209
BfaI CTAG 4 cut(s) 387, 675, 710, 714
BfuI GTATCC 2 cut(s) 344, 614
BisI GCNGC 1 cut(s) 225
BlsI GCNGC 1 cut(s) 226
Bme1390I CCNGG 3 cut(s) 58, 159, 209
Bme18I GGWCC 2 cut(s) 54, 196
BmgT120I GGNCC 2 cut(s) 54, 196
BmiI GGNNCC 4 cut(s) 55, 179, 288, 325
BmrFI CCNGG 3 cut(s) 58, 159, 209
BmsI GCATC 1 cut(s) 227
BmtI GCTAGC 1 cut(s) 717
BpiI GAAGAC 2 cut(s) 432, 512
Bpu10I CCTNAGC 1 cut(s) 200
Bpu14I TTCGAA 1 cut(s) 503
BpuEI CTTGAG 2 cut(s) 98, 323
BpuMI CCSGG 1 cut(s) 209
BsaHI GRCGYC 1 cut(s) 566
BsaJI CCNNGG 2 cut(s) 232, 328
Bsc4I CCNNNNNNNGG 1 cut(s) 283
Bse1I ACTGG 1 cut(s) 296
BseBI CCWGG 2 cut(s) 58, 159
BseDI CCNNGG 2 cut(s) 232, 328
BseGI GGATG 5 cut(s) 228, 242, 424, 439, 562
BseLI CCNNNNNNNGG 1 cut(s) 283
BseNI ACTGG 1 cut(s) 296
BseXI GCAGC 1 cut(s) 236
BsgI GTGCAG 1 cut(s) 610
Bsh1236I CGCG 2 cut(s) 194, 497
BsiSI CCGG 1 cut(s) 209
BslI CCNNNNNNNGG 1 cut(s) 283
Bsp119I TTCGAA 1 cut(s) 503
Bsp1286I GDGCHC 1 cut(s) 328
Bsp1407I TGTACA 1 cut(s) 378
Bsp143I GATC 3 cut(s) 110, 311, 658
BspACI CCGC 1 cut(s) 194
BspFNI CGCG 2 cut(s) 194, 497
BspLI GGNNCC 4 cut(s) 55, 179, 288, 325
BspOI GCTAGC 1 cut(s) 717
BspPI GGATC 1 cut(s) 666
BspT104I TTCGAA 1 cut(s) 503
BsrGI TGTACA 1 cut(s) 378
BsrI ACTGG 1 cut(s) 296
BssECI CCNNGG 2 cut(s) 232, 328
BssMI GATC 3 cut(s) 110, 311, 658
BssNI GRCGYC 1 cut(s) 566
BssT1I CCWWGG 2 cut(s) 232, 328
Bst2UI CCWGG 2 cut(s) 58, 159
Bst4CI ACNGT 1 cut(s) 24
BstACI GRCGYC 1 cut(s) 566
BstAUI TGTACA 1 cut(s) 378
BstBI TTCGAA 1 cut(s) 503
BstC8I GCNNGC 2 cut(s) 346, 715
BstDEI CTNAG 1 cut(s) 200
BstF5I GGATG 5 cut(s) 228, 242, 424, 439, 562
BstFNI CGCG 2 cut(s) 194, 497
BstKTI GATC 3 cut(s) 113, 314, 661
BstMBI GATC 3 cut(s) 110, 311, 658
BstMWI GCNNNNNNNGC 2 cut(s) 79, 738
BstNI CCWGG 2 cut(s) 58, 159
BstNSI RCATGY 1 cut(s) 348
BstSCI CCNGG 3 cut(s) 56, 157, 207
BstUI CGCG 2 cut(s) 194, 497
BstV1I GCAGC 1 cut(s) 236
BstV2I GAAGAC 2 cut(s) 432, 512
BstX2I RGATCY 1 cut(s) 658
BstYI RGATCY 1 cut(s) 658
BsuI GTATCC 2 cut(s) 344, 614
BtsCI GGATG 5 cut(s) 228, 242, 424, 439, 562
Cac8I GCNNGC 2 cut(s) 346, 715
Cfr13I GGNCC 2 cut(s) 54, 196
CseI GACGC 2 cut(s) 229, 574
Csp6I GTAC 1 cut(s) 379
CviAII CATG 5 cut(s) 83, 254, 345, 430, 646
CviQI GTAC 1 cut(s) 379
DdeI CTNAG 1 cut(s) 200
DpnI GATC 3 cut(s) 112, 313, 660
DpnII GATC 3 cut(s) 110, 311, 658
DraI TTTAAA 2 cut(s) 474, 634
Eco130I CCWWGG 2 cut(s) 232, 328
Eco24I GRGCYC 1 cut(s) 328
Eco47I GGWCC 2 cut(s) 54, 196
EcoO109I RGGNCCY 1 cut(s) 54
EcoRII CCWGG 2 cut(s) 56, 157
EcoT14I CCWWGG 2 cut(s) 232, 328
EcoT38I GRGCYC 1 cut(s) 328
ErhI CCWWGG 2 cut(s) 232, 328
FaeI CATG 5 cut(s) 86, 257, 348, 433, 649
FatI CATG 5 cut(s) 82, 253, 344, 429, 645
FblI GTMKAC 1 cut(s) 19
Fnu4HI GCNGC 1 cut(s) 225
FokI GGATG 5 cut(s) 215, 249, 431, 446, 569
FriOI GRGCYC 1 cut(s) 328
Fsp4HI GCNGC 1 cut(s) 225
FspBI CTAG 4 cut(s) 387, 675, 710, 714
GluI GCNGC 1 cut(s) 225
HapII CCGG 1 cut(s) 209
HgaI GACGC 2 cut(s) 229, 574
Hin1I GRCGYC 1 cut(s) 566
Hin1II CATG 5 cut(s) 86, 257, 348, 433, 649
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HindIII AAGCTT 3 cut(s) 139, 468, 730
HinfI GANTC 2 cut(s) 392, 698
HpaII CCGG 1 cut(s) 209
Hpy166II GTNNAC 4 cut(s) 20, 271, 379, 381
Hpy188I TCNGA 3 cut(s) 115, 245, 541
Hpy188III TCNNGA 3 cut(s) 77, 530, 675
Hpy8I GTNNAC 4 cut(s) 20, 271, 379, 381
HpyAV CCTTC 2 cut(s) 611, 649
HpyCH4III ACNGT 1 cut(s) 24
HpyCH4IV ACGT 1 cut(s) 722
HpyCH4V TGCA 4 cut(s) 224, 356, 404, 627
HpyF10VI GCNNNNNNNGC 2 cut(s) 79, 738
HpyF3I CTNAG 1 cut(s) 200
HpySE526I ACGT 1 cut(s) 722
Hsp92I GRCGYC 1 cut(s) 566
Hsp92II CATG 5 cut(s) 86, 257, 348, 433, 649
Kzo9I GATC 3 cut(s) 110, 311, 658
LmnI GCTCC 3 cut(s) 161, 183, 323
Lsp1109I GCAGC 1 cut(s) 236
LweI GCATC 1 cut(s) 227
MaeI CTAG 4 cut(s) 387, 675, 710, 714
MaeII ACGT 1 cut(s) 722
MaeIII GTNAC 5 cut(s) 28, 86, 301, 449, 562
MalI GATC 3 cut(s) 112, 313, 660
MboI GATC 3 cut(s) 110, 311, 658
MboII GAAGA 4 cut(s) 179, 182, 437, 517
MflI RGATCY 1 cut(s) 658
MhlI GDGCHC 1 cut(s) 328
MluCI AATT 4 cut(s) 533, 584, 681, 688
MlyI GAGTC 1 cut(s) 401
MmeI TCCRAC 1 cut(s) 553
MnlI CCTC 6 cut(s) 8, 29, 63, 155, 347, 479
MseI TTAA 8 cut(s) 137, 408, 465, 473, 633, 684, 725, 751
MslI CAYNNNNRTG 1 cut(s) 650
MspI CCGG 1 cut(s) 209
MspR9I CCNGG 3 cut(s) 58, 159, 209
MvaI CCWGG 2 cut(s) 58, 159
MvnI CGCG 2 cut(s) 194, 497
MwoI GCNNNNNNNGC 2 cut(s) 79, 738
NciI CCSGG 1 cut(s) 209
NdeII GATC 3 cut(s) 110, 311, 658
NheI GCTAGC 1 cut(s) 713
NlaIII CATG 5 cut(s) 86, 257, 348, 433, 649
NlaIV GGNNCC 4 cut(s) 55, 179, 288, 325
NmuCI GTSAC 3 cut(s) 301, 449, 562
NspI RCATGY 1 cut(s) 348
NspV TTCGAA 1 cut(s) 503
PaeI GCATGC 1 cut(s) 348
PfeI GAWTC 1 cut(s) 698
PkrI GCNGC 1 cut(s) 226
PleI GAGTC 1 cut(s) 400
PpsI GAGTC 1 cut(s) 400
PpuMI RGGWCCY 1 cut(s) 54
Psp5II RGGWCCY 1 cut(s) 54
Psp6I CCWGG 2 cut(s) 56, 157
PspGI CCWGG 2 cut(s) 56, 157
PspN4I GGNNCC 4 cut(s) 55, 179, 288, 325
PspPI GGNCC 2 cut(s) 54, 196
PspPPI RGGWCCY 1 cut(s) 54
PsuI RGATCY 1 cut(s) 658
RsaI GTAC 1 cut(s) 380
RsaNI GTAC 1 cut(s) 379
RseI CAYNNNNRTG 1 cut(s) 650
SaqAI TTAA 8 cut(s) 137, 408, 465, 473, 633, 684, 725, 751
SatI GCNGC 1 cut(s) 225
Sau3AI GATC 3 cut(s) 110, 311, 658
Sau96I GGNCC 2 cut(s) 54, 196
SchI GAGTC 1 cut(s) 401
ScrFI CCNGG 3 cut(s) 58, 159, 209
SduI GDGCHC 1 cut(s) 328
SfaNI GCATC 1 cut(s) 227
SfuI TTCGAA 1 cut(s) 503
SinI GGWCC 2 cut(s) 54, 196
SmiMI CAYNNNNRTG 1 cut(s) 650
SmlI CTYRAG 2 cut(s) 77, 338
SmoI CTYRAG 2 cut(s) 77, 338
SphI GCATGC 1 cut(s) 348
Sse9I AATT 4 cut(s) 533, 584, 681, 688
SsiI CCGC 1 cut(s) 194
SspI AATATT 1 cut(s) 186
SspMI CTAG 4 cut(s) 387, 675, 710, 714
StyD4I CCNGG 3 cut(s) 56, 157, 207
StyI CCWWGG 2 cut(s) 232, 328
TaaI ACNGT 1 cut(s) 24
TaiI ACGT 1 cut(s) 725
TaqI TCGA 2 cut(s) 318, 503
TasI AATT 4 cut(s) 533, 584, 681, 688
TatI WGTACW 1 cut(s) 378
TfiI GAWTC 1 cut(s) 698
Tru1I TTAA 8 cut(s) 137, 408, 465, 473, 633, 684, 725, 751
Tru9I TTAA 8 cut(s) 137, 408, 465, 473, 633, 684, 725, 751
TseFI GTSAC 3 cut(s) 301, 449, 562
TseI GCWGC 1 cut(s) 224
Tsp45I GTSAC 3 cut(s) 301, 449, 562
TspDTI ATGAA 4 cut(s) 37, 380, 662, 668
VpaK11BI GGWCC 2 cut(s) 54, 196
XapI RAATTY 3 cut(s) 533, 584, 688
XbaI TCTAGA 1 cut(s) 674
XceI RCATGY 1 cut(s) 348
XmiI GTMKAC 1 cut(s) 19
XspI CTAG 4 cut(s) 387, 675, 710, 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.