MD08G1231500.v1.1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000382
Physical Location & Seq
Reverse (-)
29791565 .. 29794817
3253 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1231500.v1.1.491

Sequence Viewer

Length: 996 bp
ATGGAAGGAGAGAGATTAGGAAGAGTAGTATGTGTAACAGGTGGTTCTGGGTACGTAGGGTCTTGGCTGGTTATGAGGCTTCTTGATCAAGGCTACTTTGTGAATACCACTGTTAGATCAGACCCTACAGAGAACAAGCGAGATCTAAGCTTTCTCACAAGTCTACCAGGAGCAACCGAAAGGCTCAAAATTTTCACCGCGGATCTCAGCAACCCGGACAGCTTCTATGCAGCCGTTGAAGGGTGCACGGGAGTGTTCCATGTTGCCACTCCAGTCGATTTCCAAGACAACGAACCTGAACAGGTAGTGACCAAAAGGTCAATTGATGGAGCCCTTGGCATTCTACAGGGATGCTTAAACGCGAAAACAGTGAAACGAGTTGTGTACACTTCTAGTGCATCCGCTGTCATGTTTGGCAGCGGCAAGGATGTGGAGGAAGTGGATGAAAGTATTTGGAGCGATATAGGTTACATTAGATCTGTAAAGCCATATGGAGGGTCATACGTGATTTCAAAGAGATTAACTGAGAAGGCAGTTCTTGAATTTTTAGAGAAATATGGGTTGGATGTTGTGACAGTAATACCTTCTTTTATTGCTGGCCCCTTCATTTGTCCTAAGCTTCCCAGCTCCGTTCAGAGCACACTGGCTATGGTCTTTGGTAAACTTGAGGAGCTTAGATTTCTTATCAACATACCATTGGTGCATGTCGATGATGTTGCCAGCGCGCACATTTTCCTTTTAGAACATCATGATGCAAAAGGGAGGTATAATTGCTCATCGCATGTCATAACCCTCATACAAATAGCCGAGTTTCTTTCTGCCAAATACCCGAAATTTCAAATACCGTCAGTAAGCTATTTAAGGGAGGTTAAAGGTGATAAAACGCCTGGTCTGTCGTCAAAGAAGCTCTTGGATTCTGGTTTCAGATTCAAGTATGGGGTTGATGAGATCCTCAGCGATGCAATTCAGTGTTGCAAAGAAAATAATTATTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00943 ko01110 map00943 map01110
Pfam Domains
Protein Families
Epimerase

Protein Analysis

332

Amino Acids

36.46

Weight (kDa)

5.93

Isoelectric Point (pI)

33.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 9 - 135 7.1e-06 NmrA-like family
Epimerase PF01370 10 - 258 2.6e-25 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 12 - 245 2.9e-16 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 12 - 264 7.4e-16 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 12 - 204 5.6e-11 Male sterility protein
NAD_binding_10 PF13460 14 - 136 1.1e-06 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45400 AT2G45400 AT2G45400 AT2G45400
fragaria_vesca FvH4_7g11750 FvH4_7g11760 FvH4_7g11780 FvH4_7g11781 FvH4_7g11781 FvH4_7g11790 FvH4_7g11790
malus_domestica MD02G1205000.v1.1 MD07G1121700.v1.1 MD08G1231300.v1.1 MD08G1231400.v1.1 MD08G1231500.v1.1
prunus_persica Prupe.2G144600_v2.0.a1 Prupe.2G144700_v2.0.a1 Prupe.2G144800_v2.0.a1
pyrus_communis pycom08g20120
rosa_chinensis RchiOBHm_Chr1g0350271 RchiOBHm_Chr1g0350281 RchiOBHm_Chr1g0350311 RchiOBHm_Chr1g0350321 RchiOBHm_Chr1g0350341 RchiOBHm_Chr1g0350361 RchiOBHm_Chr1g0350371 RchiOBHm_Chr1g0350401 RchiOBHm_Chr1g0350411 RchiOBHm_Chr1g0350431 RchiOBHm_Chr1g0350441 RchiOBHm_Chr1g0350471
rosa_laevigata RLG00000028483 RLG00000028485 RLG00000028486 RLG00000028487 RLG00000028488 RLG00000028489 RLG00000028493 RLG00000028494 RLG00000035411
rosa_multiflora Rmu_co8173820.1_g000001 Rmu_sc0001037.1_g000006 Rmu_sc0001864.1_g000002 Rmu_sc0001864.1_g000009 Rmu_ssc0000083.1_g000008 Rmu_ssc0000083.1_g000013 Rmu_ssc0000083.1_g000018
rosa_roxburghii Rroxscaffold_4G00304840 Rroxscaffold_4G00304860 Rroxscaffold_4G00304890 Rroxscaffold_4G00304900 Rroxscaffold_4G00304920 Rroxscaffold_4G00304930
rosa_rugosa Rorug01G0210800 Rorug01G0210900 Rorug01G0211000 Rorug01G0211100 Rorug01G0211200 Rorug01G0211300 Rorug01G0211300 Rorug01G0211400
rosa_samantha Rh1AG225500 Rh1AG225600 Rh1AG225700 Rh1AG225800 Rh1AG225900 Rh1AG226000 Rh1BG193400 Rh1BG193600 Rh1BG193700 Rh1BG193800 Rh1BG193900 Rh1BG194000 Rh1BG194200 Rh1CG210300 Rh1CG210500 Rh1CG210600 Rh1CG210700 Rh1CG210900 Rh1DG221700 Rh1DG221900 Rh1DG222100 Rh1DG222200 Rh1DG222300 Rh1DG222400 Rh1DG222500 Rh2BG259100 Rh3DG280100 Rh5AG528000
rosa_wichuraiana Rw0G018730 Rw0G018740 Rw0G018750 Rw0G018760 Rw0G018770 Rw0G018790 Rw1G019390 Rw1G019410 Rw1G019420 Rw1G019430 Rw1G019440 Rw1G019450 Rw1G019480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 316
AccI GTMKAC 1 cut(s) 163
AccII CGCG 3 cut(s) 200, 362, 725
AciI CCGC 4 cut(s) 198, 200, 402, 420
AclWI GGATC 2 cut(s) 210, 943
AcsI RAATTY 3 cut(s) 189, 542, 833
AfaI GTAC 2 cut(s) 53, 386
AfiI CCNNNNNNNGG 2 cut(s) 240, 494
AgsI TTSAA 5 cut(s) 239, 513, 542, 839, 931
AjnI CCWGG 2 cut(s) 166, 886
AjuI GAANNNNNNNTTGG 2 cut(s) 545, 577
AleI CACNNNNGTG 1 cut(s) 251
AluBI AGCT 7 cut(s) 150, 222, 619, 627, 673, 855, 907
AluI AGCT 7 cut(s) 150, 222, 619, 627, 673, 855, 907
Alw21I GWGCWC 2 cut(s) 248, 641
Alw44I GTGCAC 1 cut(s) 244
AlwI GGATC 2 cut(s) 210, 943
AoxI GGCC 1 cut(s) 598
ApaLI GTGCAC 1 cut(s) 244
ApeKI GCWGC 2 cut(s) 230, 417
ApoI RAATTY 3 cut(s) 189, 542, 833
AspLEI GCGC 2 cut(s) 725, 727
AspS9I GGNCC 1 cut(s) 599
AsuC2I CCSGG 1 cut(s) 215
AsuHPI GGTGA 2 cut(s) 187, 887
BaeGI GKGCMC 1 cut(s) 248
BanII GRGCYC 1 cut(s) 334
Bbv12I GWGCWC 2 cut(s) 248, 641
BbvCI CCTCAGC 1 cut(s) 953
BbvI GCAGC 2 cut(s) 242, 429
BccI CCATC 1 cut(s) 320
BceAI ACGGC 1 cut(s) 218
BcgI CGANNNNNNTGC 2 cut(s) 698, 732
BciT130I CCWGG 2 cut(s) 168, 888
BclI TGATCA 1 cut(s) 85
BcnI CCSGG 1 cut(s) 215
BfaI CTAG 1 cut(s) 393
BfmI CTRYAG 2 cut(s) 126, 344
BglII AGATCT 2 cut(s) 142, 476
BisI GCNGC 3 cut(s) 231, 418, 421
BlsI GCNGC 3 cut(s) 232, 419, 422
Bme1390I CCNGG 3 cut(s) 168, 215, 888
BmgT120I GGNCC 1 cut(s) 599
BmiI GGNNCC 2 cut(s) 331, 601
BmrFI CCNGG 3 cut(s) 168, 215, 888
BmsI GCATC 4 cut(s) 341, 407, 742, 949
BpmI CTGGAG 1 cut(s) 255
Bpu10I CCTNAGC 2 cut(s) 615, 953
BpuEI CTTGAG 1 cut(s) 686
BpuMI CCSGG 1 cut(s) 215
BsaAI YACGTR 2 cut(s) 55, 505
BsaJI CCNNGG 2 cut(s) 198, 334
Bsc4I CCNNNNNNNGG 2 cut(s) 240, 494
Bse1I ACTGG 2 cut(s) 272, 648
BseBI CCWGG 2 cut(s) 168, 888
BseDI CCNNGG 2 cut(s) 198, 334
BseGI GGATG 5 cut(s) 356, 398, 433, 448, 571
BseLI CCNNNNNNNGG 2 cut(s) 240, 494
BseMII CTCAG 3 cut(s) 220, 516, 967
BseNI ACTGG 2 cut(s) 272, 648
BsePI GCGCGC 1 cut(s) 723
BseRI GAGGAG 1 cut(s) 683
BseSI GKGCMC 1 cut(s) 248
BseXI GCAGC 2 cut(s) 242, 429
BseYI CCCAGC 1 cut(s) 623
Bsh1236I CGCG 3 cut(s) 200, 362, 725
BshFI GGCC 1 cut(s) 600
BsiHKAI GWGCWC 2 cut(s) 248, 641
BsiSI CCGG 1 cut(s) 215
BslI CCNNNNNNNGG 2 cut(s) 240, 494
BsmI GAATGC 1 cut(s) 339
BsnI GGCC 1 cut(s) 600
Bsp1286I GDGCHC 3 cut(s) 248, 334, 641
Bsp1407I TGTACA 1 cut(s) 384
Bsp143I GATC 6 cut(s) 85, 116, 142, 202, 476, 948
BspACI CCGC 4 cut(s) 198, 200, 402, 420
BspANI GGCC 1 cut(s) 600
BspCNI CTCAG 3 cut(s) 219, 517, 966
BspFNI CGCG 3 cut(s) 200, 362, 725
BspHI TCATGA 1 cut(s) 748
BspLI GGNNCC 2 cut(s) 331, 601
BspPI GGATC 2 cut(s) 210, 943
BsrGI TGTACA 1 cut(s) 384
BsrI ACTGG 2 cut(s) 272, 648
BssECI CCNNGG 2 cut(s) 198, 334
BssHII GCGCGC 1 cut(s) 723
BssMI GATC 6 cut(s) 85, 116, 142, 202, 476, 948
BssT1I CCWWGG 1 cut(s) 334
Bst2UI CCWGG 2 cut(s) 168, 888
Bst4CI ACNGT 4 cut(s) 112, 370, 577, 846
Bst6I CTCTTC 1 cut(s) 16
BstAUI TGTACA 1 cut(s) 384
BstBAI YACGTR 2 cut(s) 55, 505
BstC8I GCNNGC 3 cut(s) 598, 721, 725
BstDEI CTNAG 6 cut(s) 146, 206, 525, 615, 674, 953
BstDSI CCRYGG 1 cut(s) 198
BstF5I GGATG 5 cut(s) 356, 398, 433, 448, 571
BstFNI CGCG 3 cut(s) 200, 362, 725
BstHHI GCGC 2 cut(s) 725, 727
BstKTI GATC 6 cut(s) 88, 119, 145, 205, 479, 951
BstMBI GATC 6 cut(s) 85, 116, 142, 202, 476, 948
BstNI CCWGG 2 cut(s) 168, 888
BstNSI RCATGY 2 cut(s) 707, 785
BstSCI CCNGG 3 cut(s) 166, 213, 886
BstSFI CTRYAG 2 cut(s) 126, 344
BstSLI GKGCMC 1 cut(s) 248
BstSNI TACGTA 1 cut(s) 55
BstUI CGCG 3 cut(s) 200, 362, 725
BstV1I GCAGC 2 cut(s) 242, 429
BstX2I RGATCY 4 cut(s) 142, 202, 476, 948
BstYI RGATCY 4 cut(s) 142, 202, 476, 948
BsuRI GGCC 1 cut(s) 600
BtgI CCRYGG 1 cut(s) 198
BtgZI GCGATG 2 cut(s) 762, 972
BtsCI GGATG 5 cut(s) 356, 398, 433, 448, 571
BtsIMutI CAGTG 4 cut(s) 108, 375, 641, 974
Cac8I GCNNGC 3 cut(s) 598, 721, 725
CciI TCATGA 1 cut(s) 748
CfoI GCGC 2 cut(s) 725, 727
Cfr13I GGNCC 1 cut(s) 599
Cfr42I CCGCGG 1 cut(s) 201
Csp6I GTAC 2 cut(s) 52, 385
CviAII CATG 5 cut(s) 260, 409, 704, 749, 782
CviQI GTAC 2 cut(s) 52, 385
DdeI CTNAG 6 cut(s) 146, 206, 525, 615, 674, 953
DpnI GATC 6 cut(s) 87, 118, 144, 204, 478, 950
DpnII GATC 6 cut(s) 85, 116, 142, 202, 476, 948
DrdI GACNNNNNNGTC 1 cut(s) 316
DseDI GACNNNNNNGTC 1 cut(s) 316
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
Eco105I TACGTA 1 cut(s) 55
Eco130I CCWWGG 1 cut(s) 334
Eco24I GRGCYC 1 cut(s) 334
EcoRII CCWGG 2 cut(s) 166, 886
EcoT14I CCWWGG 1 cut(s) 334
EcoT38I GRGCYC 1 cut(s) 334
ErhI CCWWGG 1 cut(s) 334
FaeI CATG 5 cut(s) 263, 412, 707, 752, 785
FalI AAGNNNNNCTT 2 cut(s) 893, 925
FatI CATG 5 cut(s) 259, 408, 703, 748, 781
FauNDI CATATG 1 cut(s) 490
FbaI TGATCA 1 cut(s) 85
FblI GTMKAC 1 cut(s) 163
Fnu4HI GCNGC 3 cut(s) 231, 418, 421
FokI GGATG 5 cut(s) 363, 385, 440, 455, 578
FriOI GRGCYC 1 cut(s) 334
Fsp4HI GCNGC 3 cut(s) 231, 418, 421
FspBI CTAG 1 cut(s) 393
GlaI GCGC 2 cut(s) 724, 726
GluI GCNGC 3 cut(s) 231, 418, 421
GsaI CCCAGC 1 cut(s) 627
GsuI CTGGAG 1 cut(s) 255
HaeIII GGCC 1 cut(s) 600
HapII CCGG 1 cut(s) 215
HhaI GCGC 2 cut(s) 725, 727
Hin1II CATG 5 cut(s) 263, 412, 707, 752, 785
Hin6I GCGC 2 cut(s) 723, 725
HinP1I GCGC 2 cut(s) 723, 725
HindIII AAGCTT 2 cut(s) 148, 617
HinfI GANTC 2 cut(s) 914, 927
HpaII CCGG 1 cut(s) 215
HphI GGTGA 2 cut(s) 187, 887
Hpy166II GTNNAC 5 cut(s) 164, 246, 385, 387, 662
Hpy188I TCNGA 3 cut(s) 121, 636, 926
Hpy188III TCNNGA 3 cut(s) 83, 539, 749
Hpy8I GTNNAC 5 cut(s) 164, 246, 385, 387, 662
HpyAV CCTTC 4 cut(s) 233, 523, 594, 613
HpyCH4III ACNGT 4 cut(s) 112, 370, 577, 846
HpyCH4IV ACGT 2 cut(s) 54, 504
HpyCH4V TGCA 7 cut(s) 230, 246, 398, 703, 755, 962, 975
HpyF3I CTNAG 6 cut(s) 146, 206, 525, 615, 674, 953
HpySE526I ACGT 2 cut(s) 54, 504
Hsp92II CATG 5 cut(s) 263, 412, 707, 752, 785
HspAI GCGC 2 cut(s) 723, 725
Ksp22I TGATCA 1 cut(s) 85
KspI CCGCGG 1 cut(s) 201
Kzo9I GATC 6 cut(s) 85, 116, 142, 202, 476, 948
LmnI GCTCC 5 cut(s) 170, 329, 456, 632, 670
Lsp1109I GCAGC 2 cut(s) 242, 429
LweI GCATC 4 cut(s) 341, 407, 742, 949
MaeI CTAG 1 cut(s) 393
MaeII ACGT 2 cut(s) 54, 504
MaeIII GTNAC 4 cut(s) 34, 307, 467, 571
MalI GATC 6 cut(s) 87, 118, 144, 204, 478, 950
MboI GATC 6 cut(s) 85, 116, 142, 202, 476, 948
MboII GAAGA 1 cut(s) 33
MfeI CAATTG 1 cut(s) 321
MflI RGATCY 4 cut(s) 142, 202, 476, 948
MhlI GDGCHC 3 cut(s) 248, 334, 641
MluCI AATT 7 cut(s) 189, 321, 542, 769, 833, 963, 985
MmeI TCCRAC 1 cut(s) 543
MnlI CCTC 8 cut(s) 69, 427, 488, 661, 756, 803, 859, 962
MseI TTAA 4 cut(s) 356, 521, 860, 870
MslI CAYNNNNRTG 3 cut(s) 251, 708, 750
MspA1I CMGCKG 3 cut(s) 200, 404, 420
MspI CCGG 1 cut(s) 215
MspR9I CCNGG 3 cut(s) 168, 215, 888
MunI CAATTG 1 cut(s) 321
Mva1269I GAATGC 1 cut(s) 339
MvaI CCWGG 2 cut(s) 168, 888
MvnI CGCG 3 cut(s) 200, 362, 725
NciI CCSGG 1 cut(s) 215
NdeI CATATG 1 cut(s) 490
NdeII GATC 6 cut(s) 85, 116, 142, 202, 476, 948
NlaIII CATG 5 cut(s) 263, 412, 707, 752, 785
NlaIV GGNNCC 2 cut(s) 331, 601
NmeAIII GCCGAG 1 cut(s) 832
NmuCI GTSAC 2 cut(s) 307, 571
NspI RCATGY 2 cut(s) 707, 785
OliI CACNNNNGTG 1 cut(s) 251
PagI TCATGA 1 cut(s) 748
PauI GCGCGC 1 cut(s) 723
PctI GAATGC 1 cut(s) 339
PfeI GAWTC 2 cut(s) 914, 927
PkrI GCNGC 3 cut(s) 232, 419, 422
Ppu21I YACGTR 2 cut(s) 55, 505
Psp6I CCWGG 2 cut(s) 166, 886
PspFI CCCAGC 1 cut(s) 623
PspGI CCWGG 2 cut(s) 166, 886
PspN4I GGNNCC 2 cut(s) 331, 601
PspPI GGNCC 1 cut(s) 599
PsuI RGATCY 4 cut(s) 142, 202, 476, 948
PteI GCGCGC 1 cut(s) 723
RsaI GTAC 2 cut(s) 53, 386
RsaNI GTAC 2 cut(s) 52, 385
RseI CAYNNNNRTG 3 cut(s) 251, 708, 750
SacII CCGCGG 1 cut(s) 201
SaqAI TTAA 4 cut(s) 356, 521, 860, 870
SatI GCNGC 3 cut(s) 231, 418, 421
Sau3AI GATC 6 cut(s) 85, 116, 142, 202, 476, 948
Sau96I GGNCC 1 cut(s) 599
ScrFI CCNGG 3 cut(s) 168, 215, 888
SduI GDGCHC 3 cut(s) 248, 334, 641
SfaNI GCATC 4 cut(s) 341, 407, 742, 949
SfcI CTRYAG 2 cut(s) 126, 344
Sfr303I CCGCGG 1 cut(s) 201
SgrBI CCGCGG 1 cut(s) 201
SmiMI CAYNNNNRTG 3 cut(s) 251, 708, 750
SmlI CTYRAG 1 cut(s) 665
SmoI CTYRAG 1 cut(s) 665
SnaBI TACGTA 1 cut(s) 55
Sse9I AATT 7 cut(s) 189, 321, 542, 769, 833, 963, 985
SsiI CCGC 4 cut(s) 198, 200, 402, 420
SspMI CTAG 1 cut(s) 393
StyD4I CCNGG 3 cut(s) 166, 213, 886
StyI CCWWGG 1 cut(s) 334
TaaI ACNGT 4 cut(s) 112, 370, 577, 846
TaiI ACGT 2 cut(s) 57, 507
TaqI TCGA 2 cut(s) 276, 708
TasI AATT 7 cut(s) 189, 321, 542, 769, 833, 963, 985
TatI WGTACW 1 cut(s) 384
TauI GCSGC 1 cut(s) 423
TfiI GAWTC 2 cut(s) 914, 927
Tru1I TTAA 4 cut(s) 356, 521, 860, 870
Tru9I TTAA 4 cut(s) 356, 521, 860, 870
TscAI CASTG 4 cut(s) 115, 375, 648, 974
TseFI GTSAC 2 cut(s) 307, 571
TseI GCWGC 2 cut(s) 230, 417
Tsp45I GTSAC 2 cut(s) 307, 571
TspDTI ATGAA 2 cut(s) 459, 595
TspGWI ACGGA 1 cut(s) 619
TspRI CASTG 4 cut(s) 115, 375, 648, 974
VneI GTGCAC 1 cut(s) 244
XapI RAATTY 3 cut(s) 189, 542, 833
XceI RCATGY 2 cut(s) 707, 785
XmiI GTMKAC 1 cut(s) 163
XspI CTAG 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.