Rroxscaffold_4G00304840

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000382
Physical Location & Seq
Reverse (-)
25461203 .. 25465350
4148 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00304840.1

Sequence Viewer

Length: 984 bp
ATGGAAGGAGAGAAAGGTAGAGTATGTGTAACGGGAGGTTCAGGGTTCATAGGGTCCTGGTTGATTCTGAGGCTTCTTGAGCATGGTTACTATGTCAATACCACTGTTAGATCAGACCCAGAACAAAAGAAAGATGTAAGCTTCCTCACAGCTCTACCAGGAGCAAAAGAAAGGCTCCAAATTTTCAACGCGGACCTGAGCAACCCGGAAAGTTTCAATGCAGCCATCCAAGGATGCGTCGGAGTGTTCCATGTTGCTACTCCAGTTGACTTCGAAGACAAGGAACCTGAACCAGTAGTGACCAAACGATCAGTCGATGGAGCCCTTGGTATCCTCAAGGCGTGCCTATATGCAAAAACAGTGAAACGTGTTGTGTACACTTCTAGTGTATTTGCTGTTCAATTTAACAACAAAAATGTGGAAGACATGGATGAGAGTTTTTGGAGTGACACAGATTACATTAAAGCTTTAAAGCCTTATCGAGGCTCATACATGATTTCGAAGACATTGACTGAAAGAGCAGTTTTTGAATTTTCGGAGAAAAATGGATTGGATGTTGTGACAGTAATTCCTTCTTTGGTTGTTGGCCCCTTCATTTGTCCCAAGTTCCCCAGCTCTGTTCGAAGCGCATTGGCTCTGGTTTTAGGTAACAGGGATGAATATCCTATTCTTATGAATTTATCAGTGGTACATGTGGATGATGTCGCCAGAGCGCACATTTTCCTTTTCGAACATCATGATGCGAAAGGGAGATACAATTGCTCATCAGATGTCATGACAATTGAAGAAATGGCCAAGTTCCTTTCTGCTAAATTCCCCGAATTTCAAATTCCCTCAGCAGAGTCATTGAAGGAAGTTAAAGGTCCTAAGATTCCTGGTCTGTCATCAAAGAAACTCTTGGATACGGGTTTCAAATTTAAGTATGGGGTTGATGAGATGTTCAGCGAGGCAATTCGCTGCTGCAAAGAGAAGGGTTATCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00943 ko01110 map00943 map01110
Pfam Domains
Protein Families
Epimerase

Protein Analysis

327

Amino Acids

36.39

Weight (kDa)

6.34

Isoelectric Point (pI)

30.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 137 8.6e-08 NmrA-like family
Epimerase PF01370 8 - 254 1.3e-26 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 240 2.1e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 267 1.3e-14 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 196 1.3e-13 Male sterility protein
NAD_binding_10 PF13460 12 - 146 4e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45400 AT2G45400 AT2G45400 AT2G45400
fragaria_vesca FvH4_7g11750 FvH4_7g11760 FvH4_7g11780 FvH4_7g11781 FvH4_7g11781 FvH4_7g11790 FvH4_7g11790
malus_domestica MD02G1205000.v1.1 MD07G1121700.v1.1 MD08G1231300.v1.1 MD08G1231400.v1.1 MD08G1231500.v1.1
prunus_persica Prupe.2G144600_v2.0.a1 Prupe.2G144700_v2.0.a1 Prupe.2G144800_v2.0.a1
pyrus_communis pycom08g20120
rosa_chinensis RchiOBHm_Chr1g0350271 RchiOBHm_Chr1g0350281 RchiOBHm_Chr1g0350311 RchiOBHm_Chr1g0350321 RchiOBHm_Chr1g0350341 RchiOBHm_Chr1g0350361 RchiOBHm_Chr1g0350371 RchiOBHm_Chr1g0350401 RchiOBHm_Chr1g0350411 RchiOBHm_Chr1g0350431 RchiOBHm_Chr1g0350441 RchiOBHm_Chr1g0350471
rosa_laevigata RLG00000028483 RLG00000028485 RLG00000028486 RLG00000028487 RLG00000028488 RLG00000028489 RLG00000028493 RLG00000028494 RLG00000035411
rosa_multiflora Rmu_co8173820.1_g000001 Rmu_sc0001037.1_g000006 Rmu_sc0001864.1_g000002 Rmu_sc0001864.1_g000009 Rmu_ssc0000083.1_g000008 Rmu_ssc0000083.1_g000013 Rmu_ssc0000083.1_g000018
rosa_roxburghii Rroxscaffold_4G00304840 Rroxscaffold_4G00304860 Rroxscaffold_4G00304890 Rroxscaffold_4G00304900 Rroxscaffold_4G00304920 Rroxscaffold_4G00304930
rosa_rugosa Rorug01G0210800 Rorug01G0210900 Rorug01G0211000 Rorug01G0211100 Rorug01G0211200 Rorug01G0211300 Rorug01G0211300 Rorug01G0211400
rosa_samantha Rh1AG225500 Rh1AG225600 Rh1AG225700 Rh1AG225800 Rh1AG225900 Rh1AG226000 Rh1BG193400 Rh1BG193600 Rh1BG193700 Rh1BG193800 Rh1BG193900 Rh1BG194000 Rh1BG194200 Rh1CG210300 Rh1CG210500 Rh1CG210600 Rh1CG210700 Rh1CG210900 Rh1DG221700 Rh1DG221900 Rh1DG222100 Rh1DG222200 Rh1DG222300 Rh1DG222400 Rh1DG222500 Rh2BG259100 Rh3DG280100 Rh5AG528000
rosa_wichuraiana Rw0G018730 Rw0G018740 Rw0G018750 Rw0G018760 Rw0G018770 Rw0G018790 Rw1G019390 Rw1G019410 Rw1G019420 Rw1G019430 Rw1G019440 Rw1G019450 Rw1G019480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 191
AciI CCGC 1 cut(s) 191
AcoI YGGCCR 1 cut(s) 792
AcsI RAATTY 7 cut(s) 180, 530, 676, 812, 821, 828, 914
AfaI GTAC 2 cut(s) 377, 690
AfiI CCNNNNNNNGG 1 cut(s) 482
AflIII ACRYGT 2 cut(s) 367, 691
AgsI TTSAA 8 cut(s) 187, 217, 401, 530, 785, 827, 850, 913
AjnI CCWGG 3 cut(s) 56, 157, 874
AjuI GAANNNNNNNTTGG 2 cut(s) 533, 565
AluBI AGCT 4 cut(s) 141, 152, 467, 615
AluI AGCT 4 cut(s) 141, 152, 467, 615
AoxI GGCC 2 cut(s) 586, 792
ApeKI GCWGC 3 cut(s) 221, 957, 960
ApoI RAATTY 7 cut(s) 180, 530, 676, 812, 821, 828, 914
ArsI GACNNNNNNTTYG 2 cut(s) 297, 329
AspLEI GCGC 2 cut(s) 629, 715
AspS9I GGNCC 4 cut(s) 54, 193, 587, 863
AsuC2I CCSGG 1 cut(s) 206
AsuII TTCGAA 4 cut(s) 273, 500, 622, 729
AvaII GGWCC 3 cut(s) 54, 193, 863
BalI TGGCCA 1 cut(s) 794
BanII GRGCYC 1 cut(s) 325
BbsI GAAGAC 3 cut(s) 282, 429, 509
BbvCI CCTCAGC 1 cut(s) 835
BbvI GCAGC 3 cut(s) 233, 944, 947
BccI CCATC 2 cut(s) 233, 311
BciT130I CCWGG 3 cut(s) 58, 159, 876
BciVI GTATCC 2 cut(s) 341, 895
BcnI CCSGG 1 cut(s) 206
BfaI CTAG 1 cut(s) 384
BfuI GTATCC 2 cut(s) 341, 895
BisI GCNGC 3 cut(s) 222, 958, 961
BlsI GCNGC 3 cut(s) 223, 959, 962
Bme1390I CCNGG 4 cut(s) 58, 159, 206, 876
Bme18I GGWCC 3 cut(s) 54, 193, 863
BmgT120I GGNCC 4 cut(s) 54, 193, 587, 863
BmiI GGNNCC 5 cut(s) 55, 176, 285, 322, 589
BmrFI CCNGG 4 cut(s) 58, 159, 206, 876
BmsI GCATC 2 cut(s) 224, 730
BpiI GAAGAC 3 cut(s) 282, 429, 509
BpmI CTGGAG 1 cut(s) 246
Bpu10I CCTNAGC 2 cut(s) 197, 835
Bpu14I TTCGAA 4 cut(s) 273, 500, 622, 729
BpuEI CTTGAG 2 cut(s) 98, 320
BpuMI CCSGG 1 cut(s) 206
BsaBI GATNNNNATC 1 cut(s) 660
BsaJI CCNNGG 2 cut(s) 229, 325
Bsc4I CCNNNNNNNGG 1 cut(s) 482
Bse1I ACTGG 2 cut(s) 263, 293
Bse8I GATNNNNATC 1 cut(s) 660
BseBI CCWGG 3 cut(s) 58, 159, 876
BseDI CCNNGG 2 cut(s) 229, 325
BseGI GGATG 6 cut(s) 225, 239, 436, 559, 661, 703
BseJI GATNNNNATC 1 cut(s) 660
BseLI CCNNNNNNNGG 1 cut(s) 482
BseMII CTCAG 3 cut(s) 59, 188, 849
BseNI ACTGG 2 cut(s) 263, 293
BseXI GCAGC 3 cut(s) 233, 944, 947
BseYI CCCAGC 1 cut(s) 611
Bsh1236I CGCG 1 cut(s) 191
BshFI GGCC 2 cut(s) 588, 794
BsiSI CCGG 1 cut(s) 206
BslFI GGGAC 1 cut(s) 585
BslI CCNNNNNNNGG 1 cut(s) 482
BsmFI GGGAC 1 cut(s) 585
BsnI GGCC 2 cut(s) 588, 794
Bsp119I TTCGAA 4 cut(s) 273, 500, 622, 729
Bsp1286I GDGCHC 1 cut(s) 325
Bsp1407I TGTACA 1 cut(s) 375
Bsp143I GATC 2 cut(s) 110, 308
BspACI CCGC 1 cut(s) 191
BspANI GGCC 2 cut(s) 588, 794
BspCNI CTCAG 3 cut(s) 60, 189, 848
BspFNI CGCG 1 cut(s) 191
BspHI TCATGA 2 cut(s) 736, 774
BspLI GGNNCC 5 cut(s) 55, 176, 285, 322, 589
BspT104I TTCGAA 4 cut(s) 273, 500, 622, 729
BsrGI TGTACA 1 cut(s) 375
BsrI ACTGG 2 cut(s) 263, 293
BssECI CCNNGG 2 cut(s) 229, 325
BssMI GATC 2 cut(s) 110, 308
BssT1I CCWWGG 2 cut(s) 229, 325
Bst2UI CCWGG 3 cut(s) 58, 159, 876
Bst4CI ACNGT 3 cut(s) 106, 361, 565
BstAUI TGTACA 1 cut(s) 375
BstBI TTCGAA 4 cut(s) 273, 500, 622, 729
BstC8I GCNNGC 1 cut(s) 343
BstDEI CTNAG 4 cut(s) 68, 197, 835, 867
BstENI CCTNNNNNAGG 1 cut(s) 480
BstF5I GGATG 6 cut(s) 225, 239, 436, 559, 661, 703
BstFNI CGCG 1 cut(s) 191
BstHHI GCGC 2 cut(s) 629, 715
BstKTI GATC 2 cut(s) 113, 311
BstMBI GATC 2 cut(s) 110, 308
BstMWI GCNNNNNNNGC 1 cut(s) 79
BstNI CCWGG 3 cut(s) 58, 159, 876
BstNSI RCATGY 1 cut(s) 695
BstSCI CCNGG 4 cut(s) 56, 157, 204, 874
BstUI CGCG 1 cut(s) 191
BstV1I GCAGC 3 cut(s) 233, 944, 947
BstV2I GAAGAC 3 cut(s) 282, 429, 509
BsuI GTATCC 2 cut(s) 341, 895
BsuRI GGCC 2 cut(s) 588, 794
BtsCI GGATG 6 cut(s) 225, 239, 436, 559, 661, 703
BtsIMutI CAGTG 3 cut(s) 102, 366, 690
Cac8I GCNNGC 1 cut(s) 343
CciI TCATGA 2 cut(s) 736, 774
CfoI GCGC 2 cut(s) 629, 715
Cfr13I GGNCC 4 cut(s) 54, 193, 587, 863
CseI GACGC 1 cut(s) 226
Csp6I GTAC 2 cut(s) 376, 689
CviAII CATG 7 cut(s) 83, 251, 427, 493, 692, 737, 775
CviQI GTAC 2 cut(s) 376, 689
DdeI CTNAG 4 cut(s) 68, 197, 835, 867
DpnI GATC 2 cut(s) 112, 310
DpnII GATC 2 cut(s) 110, 308
DraI TTTAAA 1 cut(s) 471
EaeI YGGCCR 1 cut(s) 792
Eco130I CCWWGG 2 cut(s) 229, 325
Eco24I GRGCYC 1 cut(s) 325
Eco47I GGWCC 3 cut(s) 54, 193, 863
EcoNI CCTNNNNNAGG 1 cut(s) 480
EcoO109I RGGNCCY 2 cut(s) 54, 863
EcoRII CCWGG 3 cut(s) 56, 157, 874
EcoT14I CCWWGG 2 cut(s) 229, 325
EcoT38I GRGCYC 1 cut(s) 325
ErhI CCWWGG 2 cut(s) 229, 325
FaeI CATG 7 cut(s) 86, 254, 430, 496, 695, 740, 778
FalI AAGNNNNNCTT 2 cut(s) 881, 913
FaqI GGGAC 1 cut(s) 585
FatI CATG 7 cut(s) 82, 250, 426, 492, 691, 736, 774
Fnu4HI GCNGC 3 cut(s) 222, 958, 961
FokI GGATG 6 cut(s) 212, 246, 443, 566, 668, 710
FriOI GRGCYC 1 cut(s) 325
Fsp4HI GCNGC 3 cut(s) 222, 958, 961
FspBI CTAG 1 cut(s) 384
GlaI GCGC 2 cut(s) 628, 714
GluI GCNGC 3 cut(s) 222, 958, 961
GsaI CCCAGC 1 cut(s) 615
GsuI CTGGAG 1 cut(s) 246
HaeIII GGCC 2 cut(s) 588, 794
HapII CCGG 1 cut(s) 206
HgaI GACGC 1 cut(s) 226
HhaI GCGC 2 cut(s) 629, 715
Hin1II CATG 7 cut(s) 86, 254, 430, 496, 695, 740, 778
Hin6I GCGC 2 cut(s) 627, 713
HinP1I GCGC 2 cut(s) 627, 713
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HindIII AAGCTT 2 cut(s) 139, 465
HinfI GANTC 3 cut(s) 64, 842, 871
HpaII CCGG 1 cut(s) 206
Hpy166II GTNNAC 3 cut(s) 268, 376, 378
Hpy188I TCNGA 5 cut(s) 69, 115, 242, 538, 769
Hpy188III TCNNGA 3 cut(s) 77, 737, 775
Hpy8I GTNNAC 3 cut(s) 268, 376, 378
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 4 cut(s) 582, 601, 844, 964
HpyCH4III ACNGT 3 cut(s) 106, 361, 565
HpyCH4IV ACGT 1 cut(s) 367
HpyCH4V TGCA 3 cut(s) 221, 353, 963
HpyF10VI GCNNNNNNNGC 1 cut(s) 79
HpyF3I CTNAG 4 cut(s) 68, 197, 835, 867
HpySE526I ACGT 1 cut(s) 367
Hsp92II CATG 7 cut(s) 86, 254, 430, 496, 695, 740, 778
HspAI GCGC 2 cut(s) 627, 713
Kzo9I GATC 2 cut(s) 110, 308
LmnI GCTCC 3 cut(s) 161, 180, 320
Lsp1109I GCAGC 3 cut(s) 233, 944, 947
LweI GCATC 2 cut(s) 224, 730
MaeI CTAG 1 cut(s) 384
MaeII ACGT 1 cut(s) 367
MaeIII GTNAC 6 cut(s) 28, 86, 298, 446, 559, 647
MalI GATC 2 cut(s) 112, 310
MboI GATC 2 cut(s) 110, 308
MboII GAAGA 4 cut(s) 287, 434, 514, 797
MfeI CAATTG 2 cut(s) 757, 780
MhlI GDGCHC 1 cut(s) 325
MlsI TGGCCA 1 cut(s) 794
MluNI TGGCCA 1 cut(s) 794
MlyI GAGTC 1 cut(s) 851
MmeI TCCRAC 1 cut(s) 220
MnlI CCTC 7 cut(s) 29, 63, 155, 344, 476, 844, 940
Mox20I TGGCCA 1 cut(s) 794
MscI TGGCCA 1 cut(s) 794
MseI TTAA 5 cut(s) 405, 462, 470, 858, 918
MslI CAYNNNNRTG 2 cut(s) 696, 738
Msp20I TGGCCA 1 cut(s) 794
MspI CCGG 1 cut(s) 206
MspR9I CCNGG 4 cut(s) 58, 159, 206, 876
MunI CAATTG 2 cut(s) 757, 780
MvaI CCWGG 3 cut(s) 58, 159, 876
MvnI CGCG 1 cut(s) 191
MwoI GCNNNNNNNGC 1 cut(s) 79
NciI CCSGG 1 cut(s) 206
NdeII GATC 2 cut(s) 110, 308
NlaIII CATG 7 cut(s) 86, 254, 430, 496, 695, 740, 778
NlaIV GGNNCC 5 cut(s) 55, 176, 285, 322, 589
NmuCI GTSAC 3 cut(s) 298, 446, 559
NspI RCATGY 1 cut(s) 695
NspV TTCGAA 4 cut(s) 273, 500, 622, 729
PagI TCATGA 2 cut(s) 736, 774
PciI ACATGT 1 cut(s) 691
PfeI GAWTC 2 cut(s) 64, 871
PkrI GCNGC 3 cut(s) 223, 959, 962
PleI GAGTC 1 cut(s) 850
PpsI GAGTC 1 cut(s) 850
PpuMI RGGWCCY 2 cut(s) 54, 863
PscI ACATGT 1 cut(s) 691
Psp5II RGGWCCY 2 cut(s) 54, 863
Psp6I CCWGG 3 cut(s) 56, 157, 874
PspFI CCCAGC 1 cut(s) 611
PspGI CCWGG 3 cut(s) 56, 157, 874
PspN4I GGNNCC 5 cut(s) 55, 176, 285, 322, 589
PspPI GGNCC 4 cut(s) 54, 193, 587, 863
PspPPI RGGWCCY 2 cut(s) 54, 863
PsrI GAACNNNNNNTAC 2 cut(s) 381, 413
RsaI GTAC 2 cut(s) 377, 690
RsaNI GTAC 2 cut(s) 376, 689
RseI CAYNNNNRTG 2 cut(s) 696, 738
SaqAI TTAA 5 cut(s) 405, 462, 470, 858, 918
SatI GCNGC 3 cut(s) 222, 958, 961
Sau3AI GATC 2 cut(s) 110, 308
Sau96I GGNCC 4 cut(s) 54, 193, 587, 863
SchI GAGTC 1 cut(s) 851
ScrFI CCNGG 4 cut(s) 58, 159, 206, 876
SduI GDGCHC 1 cut(s) 325
SfaNI GCATC 2 cut(s) 224, 730
SfuI TTCGAA 4 cut(s) 273, 500, 622, 729
SinI GGWCC 3 cut(s) 54, 193, 863
SmiMI CAYNNNNRTG 2 cut(s) 696, 738
SmlI CTYRAG 2 cut(s) 77, 335
SmoI CTYRAG 2 cut(s) 77, 335
SsiI CCGC 1 cut(s) 191
SspMI CTAG 1 cut(s) 384
StyD4I CCNGG 4 cut(s) 56, 157, 204, 874
StyI CCWWGG 2 cut(s) 229, 325
TaaI ACNGT 3 cut(s) 106, 361, 565
TaiI ACGT 1 cut(s) 370
TaqI TCGA 6 cut(s) 273, 315, 481, 500, 622, 729
TatI WGTACW 1 cut(s) 375
TfiI GAWTC 2 cut(s) 64, 871
Tru1I TTAA 5 cut(s) 405, 462, 470, 858, 918
Tru9I TTAA 5 cut(s) 405, 462, 470, 858, 918
TscAI CASTG 3 cut(s) 109, 366, 690
TseFI GTSAC 3 cut(s) 298, 446, 559
TseI GCWGC 3 cut(s) 221, 957, 960
Tsp45I GTSAC 3 cut(s) 298, 446, 559
TspDTI ATGAA 4 cut(s) 37, 583, 672, 689
TspRI CASTG 3 cut(s) 109, 366, 690
VpaK11BI GGWCC 3 cut(s) 54, 193, 863
XagI CCTNNNNNAGG 1 cut(s) 480
XapI RAATTY 7 cut(s) 180, 530, 676, 812, 821, 828, 914
XceI RCATGY 1 cut(s) 695
XspI CTAG 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.