FvH4_7g11760

3-beta hydroxysteroid dehydrogenase/isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000382
Physical Location & Seq
Forward (+)
10793449 .. 10795182
1734 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g11760.t1

Sequence Viewer

Length: 891 bp
ATGGAGGCAGAAAAGGGGCCAGTTTGTGTAACAGGTGGAGCTGGATTCATCGGCTCATGGTTAGTTATGAGGCTTCTGCAAAACGGTTACACTGTTCGAACTACTGTTAGACCTGACCCAGAATGCAAGAGAGACATCAGCTACCTCACAAGCCTACCAGGAGCATCAGATAAGCTTCACATCTTCAATGCAGATCTAGACCAACCTGAAAGTTTCAATGCAGCAATTGAAGGATGCACTGGAGTCTTCCATGTTGCCCATCCTAATCCTCTGAAAGAACAAGATGAAGAAATTGTAACCAAAAAATCAGTAGAAGGCACTCTAGGCATACTGAGAGCCTGCCTGAAGTCCAAGACTGTAAAAAGGGTTGTATACACTTCTAGTGCAGCAACAATTTCTCATAGCGGAACCAACAAAGACATTGTGGATGAGAGTACATGGACTGACGTTGAATATCATAGGTCTCTGAAAGTACGTGAGACTCCATATGTGGCTGCAAAGACCAAGACAGAGCAAGCCGCTCTGGAATTTGCTGAGAAAAATGGATTGGAAATTGTCACTTTGATCCCCCCATTAGTGATTGGTGGATTTCTCTGCAATACACTCCCCAGCTCTGTATATTTGTTCCTATGCATGATTCAAGGAAATGAACATCTGTATGGATATCTGAAAAACAAAAGTATGGTACACATAGATGATTTGGTTAGTGCACACATCTTTCTTTTCGAAAACCCTGATGCAAAAGGGAGTTCCTTGAAGGGCATTGAAGGTCACAAAATGTGTGGGTACTCATCGCAGAAGCTCCTTAATTCTGGGTTCAAGTTTAAGAATGGGTTAGAAGACATGTTCGATGGAGCAGTTCAGTCTTGCAGAGAAAAGGGATTTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

297

Amino Acids

32.52

Weight (kDa)

6.32

Isoelectric Point (pI)

35.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 135 1.3e-07 NmrA-like family
Epimerase PF01370 8 - 249 2.8e-27 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 196 1.6e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 240 6.3e-14 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 200 1.5e-10 Male sterility protein
NAD_binding_10 PF13460 12 - 132 2.2e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45400 AT2G45400 AT2G45400 AT2G45400
fragaria_vesca FvH4_7g11750 FvH4_7g11760 FvH4_7g11780 FvH4_7g11781 FvH4_7g11781 FvH4_7g11790 FvH4_7g11790
malus_domestica MD02G1205000.v1.1 MD07G1121700.v1.1 MD08G1231300.v1.1 MD08G1231400.v1.1 MD08G1231500.v1.1
prunus_persica Prupe.2G144600_v2.0.a1 Prupe.2G144700_v2.0.a1 Prupe.2G144800_v2.0.a1
pyrus_communis pycom08g20120
rosa_chinensis RchiOBHm_Chr1g0350271 RchiOBHm_Chr1g0350281 RchiOBHm_Chr1g0350311 RchiOBHm_Chr1g0350321 RchiOBHm_Chr1g0350341 RchiOBHm_Chr1g0350361 RchiOBHm_Chr1g0350371 RchiOBHm_Chr1g0350401 RchiOBHm_Chr1g0350411 RchiOBHm_Chr1g0350431 RchiOBHm_Chr1g0350441 RchiOBHm_Chr1g0350471
rosa_laevigata RLG00000028483 RLG00000028485 RLG00000028486 RLG00000028487 RLG00000028488 RLG00000028489 RLG00000028493 RLG00000028494 RLG00000035411
rosa_multiflora Rmu_co8173820.1_g000001 Rmu_sc0001037.1_g000006 Rmu_sc0001864.1_g000002 Rmu_sc0001864.1_g000009 Rmu_ssc0000083.1_g000008 Rmu_ssc0000083.1_g000013 Rmu_ssc0000083.1_g000018
rosa_roxburghii Rroxscaffold_4G00304840 Rroxscaffold_4G00304860 Rroxscaffold_4G00304890 Rroxscaffold_4G00304900 Rroxscaffold_4G00304920 Rroxscaffold_4G00304930
rosa_rugosa Rorug01G0210800 Rorug01G0210900 Rorug01G0211000 Rorug01G0211100 Rorug01G0211200 Rorug01G0211300 Rorug01G0211300 Rorug01G0211400
rosa_samantha Rh1AG225500 Rh1AG225600 Rh1AG225700 Rh1AG225800 Rh1AG225900 Rh1AG226000 Rh1BG193400 Rh1BG193600 Rh1BG193700 Rh1BG193800 Rh1BG193900 Rh1BG194000 Rh1BG194200 Rh1CG210300 Rh1CG210500 Rh1CG210600 Rh1CG210700 Rh1CG210900 Rh1DG221700 Rh1DG221900 Rh1DG222100 Rh1DG222200 Rh1DG222300 Rh1DG222400 Rh1DG222500 Rh2BG259100 Rh3DG280100 Rh5AG528000
rosa_wichuraiana Rw0G018730 Rw0G018740 Rw0G018750 Rw0G018760 Rw0G018770 Rw0G018790 Rw1G019390 Rw1G019410 Rw1G019420 Rw1G019430 Rw1G019440 Rw1G019450 Rw1G019480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 521
AccI GTMKAC 1 cut(s) 372
AciI CCGC 2 cut(s) 405, 519
AclWI GGATC 1 cut(s) 559
AcsI RAATTY 1 cut(s) 527
AcuI CTGAAG 1 cut(s) 365
AfaI GTAC 4 cut(s) 436, 474, 687, 788
AflIII ACRYGT 1 cut(s) 843
AgsI TTSAA 8 cut(s) 187, 217, 230, 452, 641, 757, 767, 820
AjnI CCWGG 1 cut(s) 157
AjuI GAANNNNNNNTTGG 2 cut(s) 530, 562
AluBI AGCT 5 cut(s) 41, 141, 175, 612, 802
AluI AGCT 5 cut(s) 41, 141, 175, 612, 802
Alw21I GWGCWC 1 cut(s) 712
Alw26I GTCTC 3 cut(s) 126, 468, 473
Alw44I GTGCAC 1 cut(s) 708
AlwI GGATC 1 cut(s) 559
AoxI GGCC 1 cut(s) 17
ApaLI GTGCAC 1 cut(s) 708
ApeKI GCWGC 3 cut(s) 221, 386, 494
ApoI RAATTY 1 cut(s) 527
AspS9I GGNCC 1 cut(s) 17
AsuII TTCGAA 2 cut(s) 97, 726
BaeGI GKGCMC 1 cut(s) 712
BbsI GAAGAC 2 cut(s) 238, 846
Bbv12I GWGCWC 1 cut(s) 712
BbvI GCAGC 3 cut(s) 233, 398, 481
BccI CCATC 2 cut(s) 267, 845
BciT130I CCWGG 1 cut(s) 159
BcoDI GTCTC 3 cut(s) 126, 468, 473
BfaI CTAG 3 cut(s) 197, 323, 381
BglII AGATCT 1 cut(s) 193
BisI GCNGC 4 cut(s) 222, 387, 495, 519
BlsI GCNGC 4 cut(s) 223, 388, 496, 520
Bme1390I CCNGG 1 cut(s) 159
BmgT120I GGNCC 1 cut(s) 17
BmiI GGNNCC 2 cut(s) 18, 409
BmrFI CCNGG 1 cut(s) 159
BmsI GCATC 3 cut(s) 173, 224, 727
BpiI GAAGAC 2 cut(s) 238, 846
BpmI CTGGAG 1 cut(s) 261
Bpu14I TTCGAA 2 cut(s) 97, 726
BsaAI YACGTR 1 cut(s) 476
BsaI GGTCTC 1 cut(s) 468
Bse1I ACTGG 2 cut(s) 20, 244
BseBI CCWGG 1 cut(s) 159
BseGI GGATG 3 cut(s) 239, 259, 433
BseMII CTCAG 2 cut(s) 323, 525
BseNI ACTGG 2 cut(s) 20, 244
BseSI GKGCMC 1 cut(s) 712
BseXI GCAGC 3 cut(s) 233, 398, 481
BseYI CCCAGC 1 cut(s) 608
BsgI GTGCAG 1 cut(s) 405
BshFI GGCC 1 cut(s) 19
BsiHKAI GWGCWC 1 cut(s) 712
BsmAI GTCTC 3 cut(s) 126, 468, 473
BsmI GAATGC 1 cut(s) 128
BsnI GGCC 1 cut(s) 19
Bso31I GGTCTC 1 cut(s) 468
Bsp119I TTCGAA 2 cut(s) 97, 726
Bsp1286I GDGCHC 1 cut(s) 712
Bsp143I GATC 2 cut(s) 193, 564
BspACI CCGC 2 cut(s) 405, 519
BspANI GGCC 1 cut(s) 19
BspCNI CTCAG 2 cut(s) 324, 526
BspLI GGNNCC 2 cut(s) 18, 409
BspPI GGATC 1 cut(s) 559
BspT104I TTCGAA 2 cut(s) 97, 726
BspTNI GGTCTC 1 cut(s) 468
BsrBI CCGCTC 1 cut(s) 521
BsrI ACTGG 2 cut(s) 20, 244
BssMI GATC 2 cut(s) 193, 564
BssNAI GTATAC 1 cut(s) 373
Bst1107I GTATAC 1 cut(s) 373
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 4 cut(s) 86, 94, 106, 358
BstBAI YACGTR 1 cut(s) 476
BstBI TTCGAA 2 cut(s) 97, 726
BstC8I GCNNGC 2 cut(s) 340, 516
BstDEI CTNAG 2 cut(s) 332, 534
BstF5I GGATG 3 cut(s) 239, 259, 433
BstKTI GATC 2 cut(s) 196, 567
BstMAI GTCTC 3 cut(s) 126, 468, 473
BstMBI GATC 2 cut(s) 193, 564
BstMWI GCNNNNNNNGC 1 cut(s) 324
BstNI CCWGG 1 cut(s) 159
BstNSI RCATGY 1 cut(s) 847
BstSCI CCNGG 1 cut(s) 157
BstSLI GKGCMC 1 cut(s) 712
BstV1I GCAGC 3 cut(s) 233, 398, 481
BstV2I GAAGAC 2 cut(s) 238, 846
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
BstZ17I GTATAC 1 cut(s) 373
BsuRI GGCC 1 cut(s) 19
BtgZI GCGATG 1 cut(s) 777
BtsCI GGATG 3 cut(s) 239, 259, 433
BtsIMutI CAGTG 2 cut(s) 90, 237
Cac8I GCNNGC 2 cut(s) 340, 516
Cfr13I GGNCC 1 cut(s) 17
Csp6I GTAC 4 cut(s) 435, 473, 686, 787
CspCI CAANNNNNGTGG 2 cut(s) 763, 798
CviAII CATG 5 cut(s) 57, 251, 438, 634, 844
CviQI GTAC 4 cut(s) 435, 473, 686, 787
DdeI CTNAG 2 cut(s) 332, 534
DpnI GATC 2 cut(s) 195, 566
DpnII GATC 2 cut(s) 193, 564
Eco31I GGTCTC 1 cut(s) 468
Eco32I GATATC 1 cut(s) 665
Eco57I CTGAAG 1 cut(s) 365
EcoRII CCWGG 1 cut(s) 157
EcoRV GATATC 1 cut(s) 665
EcoT22I ATGCAT 1 cut(s) 635
FaeI CATG 5 cut(s) 60, 254, 441, 637, 847
FatI CATG 5 cut(s) 56, 250, 437, 633, 843
FauNDI CATATG 1 cut(s) 487
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 4 cut(s) 222, 387, 495, 519
FokI GGATG 3 cut(s) 246, 246, 440
Fsp4HI GCNGC 4 cut(s) 222, 387, 495, 519
FspBI CTAG 3 cut(s) 197, 323, 381
GluI GCNGC 4 cut(s) 222, 387, 495, 519
GsaI CCCAGC 1 cut(s) 612
GsuI CTGGAG 1 cut(s) 261
HaeIII GGCC 1 cut(s) 19
Hin1II CATG 5 cut(s) 60, 254, 441, 637, 847
HindIII AAGCTT 1 cut(s) 173
HinfI GANTC 4 cut(s) 45, 243, 481, 637
Hpy166II GTNNAC 3 cut(s) 373, 688, 710
Hpy188I TCNGA 4 cut(s) 169, 273, 468, 669
Hpy188III TCNNGA 2 cut(s) 197, 524
Hpy8I GTNNAC 3 cut(s) 373, 688, 710
HpyAV CCTTC 4 cut(s) 224, 308, 751, 761
HpyCH4III ACNGT 4 cut(s) 86, 94, 106, 358
HpyCH4IV ACGT 2 cut(s) 447, 475
HpyF10VI GCNNNNNNNGC 1 cut(s) 324
HpyF3I CTNAG 2 cut(s) 332, 534
HpySE526I ACGT 2 cut(s) 447, 475
Hsp92II CATG 5 cut(s) 60, 254, 441, 637, 847
Kzo9I GATC 2 cut(s) 193, 564
LmnI GCTCC 4 cut(s) 38, 161, 807, 854
Lsp1109I GCAGC 3 cut(s) 233, 398, 481
LweI GCATC 3 cut(s) 173, 224, 727
MaeI CTAG 3 cut(s) 197, 323, 381
MaeII ACGT 2 cut(s) 447, 475
MaeIII GTNAC 5 cut(s) 28, 86, 295, 556, 770
MalI GATC 2 cut(s) 195, 566
MbiI CCGCTC 1 cut(s) 521
MboI GATC 2 cut(s) 193, 564
MboII GAAGA 4 cut(s) 175, 238, 299, 851
MfeI CAATTG 1 cut(s) 225
MflI RGATCY 1 cut(s) 193
MhlI GDGCHC 1 cut(s) 712
MluCI AATT 6 cut(s) 225, 291, 393, 527, 552, 808
MlyI GAGTC 2 cut(s) 252, 475
MnlI CCTC 3 cut(s) 63, 155, 279
Mph1103I ATGCAT 1 cut(s) 635
MseI TTAA 2 cut(s) 807, 825
MslI CAYNNNNRTG 2 cut(s) 657, 693
MspR9I CCNGG 1 cut(s) 159
MunI CAATTG 1 cut(s) 225
Mva1269I GAATGC 1 cut(s) 128
MvaI CCWGG 1 cut(s) 159
MwoI GCNNNNNNNGC 1 cut(s) 324
NdeI CATATG 1 cut(s) 487
NdeII GATC 2 cut(s) 193, 564
NlaIII CATG 5 cut(s) 60, 254, 441, 637, 847
NlaIV GGNNCC 2 cut(s) 18, 409
NmuCI GTSAC 2 cut(s) 556, 770
NsiI ATGCAT 1 cut(s) 635
NspI RCATGY 1 cut(s) 847
NspV TTCGAA 2 cut(s) 97, 726
PciI ACATGT 1 cut(s) 843
PctI GAATGC 1 cut(s) 128
PfeI GAWTC 2 cut(s) 45, 637
PkrI GCNGC 4 cut(s) 223, 388, 496, 520
PleI GAGTC 2 cut(s) 251, 475
PpsI GAGTC 2 cut(s) 251, 475
Ppu21I YACGTR 1 cut(s) 476
PscI ACATGT 1 cut(s) 843
Psp6I CCWGG 1 cut(s) 157
PspFI CCCAGC 1 cut(s) 608
PspGI CCWGG 1 cut(s) 157
PspN4I GGNNCC 2 cut(s) 18, 409
PspPI GGNCC 1 cut(s) 17
PsuI RGATCY 1 cut(s) 193
RsaI GTAC 4 cut(s) 436, 474, 687, 788
RsaNI GTAC 4 cut(s) 435, 473, 686, 787
RseI CAYNNNNRTG 2 cut(s) 657, 693
SaqAI TTAA 2 cut(s) 807, 825
SatI GCNGC 4 cut(s) 222, 387, 495, 519
Sau3AI GATC 2 cut(s) 193, 564
Sau96I GGNCC 1 cut(s) 17
SchI GAGTC 2 cut(s) 252, 475
ScrFI CCNGG 1 cut(s) 159
SduI GDGCHC 1 cut(s) 712
SfaNI GCATC 3 cut(s) 173, 224, 727
SfuI TTCGAA 2 cut(s) 97, 726
SmiMI CAYNNNNRTG 2 cut(s) 657, 693
Sse9I AATT 6 cut(s) 225, 291, 393, 527, 552, 808
SsiI CCGC 2 cut(s) 405, 519
SspMI CTAG 3 cut(s) 197, 323, 381
StyD4I CCNGG 1 cut(s) 157
TaaI ACNGT 4 cut(s) 86, 94, 106, 358
TaiI ACGT 2 cut(s) 450, 478
TaqI TCGA 3 cut(s) 97, 726, 849
TasI AATT 6 cut(s) 225, 291, 393, 527, 552, 808
TatI WGTACW 1 cut(s) 434
TauI GCSGC 1 cut(s) 521
TfiI GAWTC 2 cut(s) 45, 637
Tru1I TTAA 2 cut(s) 807, 825
Tru9I TTAA 2 cut(s) 807, 825
TscAI CASTG 2 cut(s) 97, 244
TseFI GTSAC 2 cut(s) 556, 770
TseI GCWGC 3 cut(s) 221, 386, 494
Tsp45I GTSAC 2 cut(s) 556, 770
TspDTI ATGAA 3 cut(s) 37, 300, 663
TspRI CASTG 2 cut(s) 97, 244
VneI GTGCAC 1 cut(s) 708
XapI RAATTY 1 cut(s) 527
XbaI TCTAGA 1 cut(s) 196
XceI RCATGY 1 cut(s) 847
XmiI GTMKAC 1 cut(s) 372
XspI CTAG 3 cut(s) 197, 323, 381
Zsp2I ATGCAT 1 cut(s) 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.