Encoded by
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 710 |
| AccI | GTMKAC | 1 cut(s) | 145 |
| AccII | CGCG | 2 cut(s) | 139, 141 |
| AciI | CCGC | 2 cut(s) | 137, 141 |
| AclWI | GGATC | 3 cut(s) | 299, 941, 1098 |
| AcoI | YGGCCR | 2 cut(s) | 869, 1037 |
| AcsI | RAATTY | 4 cut(s) | 130, 453, 658, 834 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 710, 956 |
| AflII | CTTAAG | 1 cut(s) | 236 |
| AgsI | TTSAA | 5 cut(s) | 279, 458, 631, 639, 1069 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 467 |
| AjnI | CCWGG | 1 cut(s) | 1206 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 240, 272 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 591, 623 |
| Alw21I | GWGCWC | 1 cut(s) | 402 |
| Alw26I | GTCTC | 1 cut(s) | 536 |
| AlwI | GGATC | 3 cut(s) | 299, 941, 1098 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 59, 98 |
| AoxI | GGCC | 5 cut(s) | 701, 781, 869, 1037, 1173 |
| ApeKI | GCWGC | 4 cut(s) | 78, 569, 758, 980 |
| ApoI | RAATTY | 4 cut(s) | 130, 453, 658, 834 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 8 |
| AspLEI | GCGC | 1 cut(s) | 141 |
| AspS9I | GGNCC | 3 cut(s) | 701, 781, 1173 |
| AsuHPI | GGTGA | 3 cut(s) | 126, 913, 1196 |
| AsuNHI | GCTAGC | 1 cut(s) | 688 |
| BarI | GAAGNNNNNNTAC | 1 cut(s) | 28 |
| BbsI | GAAGAC | 1 cut(s) | 108 |
| Bbv12I | GWGCWC | 1 cut(s) | 402 |
| BbvCI | CCTCAGC | 1 cut(s) | 203 |
| BbvI | GCAGC | 4 cut(s) | 90, 556, 745, 992 |
| BccI | CCATC | 2 cut(s) | 434, 792 |
| BceAI | ACGGC | 1 cut(s) | 358 |
| BciT130I | CCWGG | 1 cut(s) | 1208 |
| BciVI | GTATCC | 1 cut(s) | 419 |
| BcoDI | GTCTC | 1 cut(s) | 536 |
| BfaI | CTAG | 3 cut(s) | 689, 1217, 1239 |
| BfmI | CTRYAG | 1 cut(s) | 732 |
| BfrI | CTTAAG | 1 cut(s) | 236 |
| BfuI | GTATCC | 1 cut(s) | 419 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1001 |
| BisI | GCNGC | 4 cut(s) | 79, 570, 759, 981 |
| BlpI | GCTNAGC | 1 cut(s) | 124 |
| BlsI | GCNGC | 4 cut(s) | 80, 571, 760, 982 |
| Bme1390I | CCNGG | 1 cut(s) | 1208 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 467 |
| BmgT120I | GGNCC | 3 cut(s) | 701, 781, 1173 |
| BmiI | GGNNCC | 2 cut(s) | 702, 1174 |
| BmrFI | CCNGG | 1 cut(s) | 1208 |
| BmrI | ACTGGG | 1 cut(s) | 572 |
| BmtI | GCTAGC | 1 cut(s) | 692 |
| BmuI | ACTGGG | 1 cut(s) | 572 |
| BpiI | GAAGAC | 1 cut(s) | 108 |
| BpmI | CTGGAG | 2 cut(s) | 747, 829 |
| Bpu10I | CCTNAGC | 2 cut(s) | 60, 203 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 124 |
| BpuEI | CTTGAG | 1 cut(s) | 558 |
| BsaJI | CCNNGG | 2 cut(s) | 297, 536 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 802, 832, 1212, 1242 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 710, 956 |
| Bse118I | RCCGGY | 2 cut(s) | 867, 1035 |
| Bse1I | ACTGG | 2 cut(s) | 308, 567 |
| Bse3DI | GCAATG | 1 cut(s) | 771 |
| BseBI | CCWGG | 1 cut(s) | 1208 |
| BseDI | CCNNGG | 2 cut(s) | 297, 536 |
| BseGI | GGATG | 2 cut(s) | 445, 658 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 710, 956 |
| BseMI | GCAATG | 1 cut(s) | 771 |
| BseMII | CTCAG | 7 cut(s) | 51, 138, 194, 282, 909, 1101, 1191 |
| BseNI | ACTGG | 2 cut(s) | 308, 567 |
| BseRI | GAGGAG | 1 cut(s) | 1175 |
| BseXI | GCAGC | 4 cut(s) | 90, 556, 745, 992 |
| BsgI | GTGCAG | 1 cut(s) | 999 |
| Bsh1236I | CGCG | 2 cut(s) | 139, 141 |
| BshFI | GGCC | 5 cut(s) | 703, 783, 871, 1039, 1175 |
| BsiHKAI | GWGCWC | 1 cut(s) | 402 |
| BsiSI | CCGG | 2 cut(s) | 868, 1036 |
| BslFI | GGGAC | 1 cut(s) | 1165 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 710, 956 |
| BsmAI | GTCTC | 1 cut(s) | 536 |
| BsmFI | GGGAC | 1 cut(s) | 1165 |
| BsnI | GGCC | 5 cut(s) | 703, 783, 871, 1039, 1175 |
| Bsp1286I | GDGCHC | 1 cut(s) | 402 |
| Bsp143I | GATC | 6 cut(s) | 50, 304, 502, 851, 946, 1103 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 124 |
| BspACI | CCGC | 2 cut(s) | 137, 141 |
| BspANI | GGCC | 5 cut(s) | 703, 783, 871, 1039, 1175 |
| BspCNI | CTCAG | 7 cut(s) | 52, 137, 195, 283, 910, 1100, 1190 |
| BspFNI | CGCG | 2 cut(s) | 139, 141 |
| BspHI | TCATGA | 2 cut(s) | 592, 1254 |
| BspLI | GGNNCC | 2 cut(s) | 702, 1174 |
| BspMAI | CTGCAG | 1 cut(s) | 736 |
| BspOI | GCTAGC | 1 cut(s) | 692 |
| BspPI | GGATC | 3 cut(s) | 299, 941, 1098 |
| BspTI | CTTAAG | 1 cut(s) | 236 |
| BsrDI | GCAATG | 1 cut(s) | 771 |
| BsrFI | RCCGGY | 2 cut(s) | 867, 1035 |
| BsrI | ACTGG | 2 cut(s) | 308, 567 |
| BssAI | RCCGGY | 2 cut(s) | 867, 1035 |
| BssECI | CCNNGG | 2 cut(s) | 297, 536 |
| BssMI | GATC | 6 cut(s) | 50, 304, 502, 851, 946, 1103 |
| BssNAI | GTATAC | 1 cut(s) | 146 |
| BssT1I | CCWWGG | 2 cut(s) | 297, 536 |
| Bst1107I | GTATAC | 1 cut(s) | 146 |
| Bst2UI | CCWGG | 1 cut(s) | 1208 |
| Bst4CI | ACNGT | 2 cut(s) | 431, 909 |
| BstAFI | CTTAAG | 1 cut(s) | 236 |
| BstC8I | GCNNGC | 5 cut(s) | 210, 288, 690, 848, 869 |
| BstF5I | GGATG | 2 cut(s) | 445, 658 |
| BstFNI | CGCG | 2 cut(s) | 139, 141 |
| BstHHI | GCGC | 1 cut(s) | 141 |
| BstKTI | GATC | 6 cut(s) | 53, 307, 505, 854, 949, 1106 |
| BstMAI | GTCTC | 1 cut(s) | 536 |
| BstMBI | GATC | 6 cut(s) | 50, 304, 502, 851, 946, 1103 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 1001 |
| BstNI | CCWGG | 1 cut(s) | 1208 |
| BstNSI | RCATGY | 1 cut(s) | 1229 |
| BstSCI | CCNGG | 1 cut(s) | 1206 |
| BstSFI | CTRYAG | 1 cut(s) | 732 |
| BstUI | CGCG | 2 cut(s) | 139, 141 |
| BstV1I | GCAGC | 4 cut(s) | 90, 556, 745, 992 |
| BstV2I | GAAGAC | 1 cut(s) | 108 |
| BstX2I | RGATCY | 1 cut(s) | 946 |
| BstYI | RGATCY | 1 cut(s) | 946 |
| BstZ17I | GTATAC | 1 cut(s) | 146 |
| BsuI | GTATCC | 1 cut(s) | 419 |
| BsuRI | GGCC | 5 cut(s) | 703, 783, 871, 1039, 1175 |
| BtsCI | GGATG | 2 cut(s) | 445, 658 |
| BtsI | GCAGTG | 1 cut(s) | 982 |
| BtsIMutI | CAGTG | 1 cut(s) | 982 |
| Cac8I | GCNNGC | 5 cut(s) | 210, 288, 690, 848, 869 |
| CaiI | CAGNNNCTG | 2 cut(s) | 59, 98 |
| CciI | TCATGA | 2 cut(s) | 592, 1254 |
| CfoI | GCGC | 1 cut(s) | 141 |
| Cfr10I | RCCGGY | 2 cut(s) | 867, 1035 |
| Cfr13I | GGNCC | 3 cut(s) | 701, 781, 1173 |
| CviAII | CATG | 4 cut(s) | 593, 722, 1226, 1255 |
| DpnI | GATC | 6 cut(s) | 52, 306, 504, 853, 948, 1105 |
| DpnII | GATC | 6 cut(s) | 50, 304, 502, 851, 946, 1103 |
| DraI | TTTAAA | 1 cut(s) | 664 |
| DriI | GACNNNNNGTC | 1 cut(s) | 467 |
| EaeI | YGGCCR | 2 cut(s) | 869, 1037 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 467 |
| Eco130I | CCWWGG | 2 cut(s) | 297, 536 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1173 |
| EcoRI | GAATTC | 1 cut(s) | 453 |
| EcoRII | CCWGG | 1 cut(s) | 1206 |
| EcoT14I | CCWWGG | 2 cut(s) | 297, 536 |
| ErhI | CCWWGG | 2 cut(s) | 297, 536 |
| FaeI | CATG | 4 cut(s) | 596, 725, 1229, 1258 |
| FaqI | GGGAC | 1 cut(s) | 1165 |
| FatI | CATG | 4 cut(s) | 592, 721, 1225, 1254 |
| FauNDI | CATATG | 1 cut(s) | 748 |
| FblI | GTMKAC | 1 cut(s) | 145 |
| Fnu4HI | GCNGC | 4 cut(s) | 79, 570, 759, 981 |
| FokI | GGATG | 2 cut(s) | 452, 665 |
| Fsp4HI | GCNGC | 4 cut(s) | 79, 570, 759, 981 |
| FspBI | CTAG | 3 cut(s) | 689, 1217, 1239 |
| GlaI | GCGC | 1 cut(s) | 140 |
| GluI | GCNGC | 4 cut(s) | 79, 570, 759, 981 |
| GsuI | CTGGAG | 2 cut(s) | 747, 829 |
| HaeIII | GGCC | 5 cut(s) | 703, 783, 871, 1039, 1175 |
| HapII | CCGG | 2 cut(s) | 868, 1036 |
| HhaI | GCGC | 1 cut(s) | 141 |
| Hin1II | CATG | 4 cut(s) | 596, 725, 1229, 1258 |
| Hin6I | GCGC | 1 cut(s) | 139 |
| HinP1I | GCGC | 1 cut(s) | 139 |
| HincII | GTYRAC | 2 cut(s) | 729, 1144 |
| HindII | GTYRAC | 2 cut(s) | 729, 1144 |
| HindIII | AAGCTT | 1 cut(s) | 671 |
| HinfI | GANTC | 9 cut(s) | 293, 406, 1048, 1085, 1121, 1145, 1181, 1201, 1251 |
| HpaII | CCGG | 2 cut(s) | 868, 1036 |
| HphI | GGTGA | 3 cut(s) | 126, 913, 1196 |
| Hpy166II | GTNNAC | 4 cut(s) | 146, 189, 729, 1144 |
| Hpy188I | TCNGA | 6 cut(s) | 85, 679, 789, 1090, 1108, 1180 |
| Hpy188III | TCNNGA | 9 cut(s) | 59, 93, 593, 623, 764, 808, 917, 1118, 1255 |
| Hpy8I | GTNNAC | 4 cut(s) | 146, 189, 729, 1144 |
| Hpy99I | CGWCG | 2 cut(s) | 344, 781 |
| HpyAV | CCTTC | 3 cut(s) | 242, 590, 1088 |
| HpyCH4III | ACNGT | 2 cut(s) | 431, 909 |
| HpyCH4V | TGCA | 4 cut(s) | 734, 980, 1028, 1264 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 1001 |
| Hsp92II | CATG | 4 cut(s) | 596, 725, 1229, 1258 |
| HspAI | GCGC | 1 cut(s) | 139 |
| KroI | GCCGGC | 1 cut(s) | 867 |
| KroNI | GCCGGC | 1 cut(s) | 869 |
| Kzo9I | GATC | 6 cut(s) | 50, 304, 502, 851, 946, 1103 |
| LmnI | GCTCC | 7 cut(s) | 61, 95, 397, 566, 766, 810, 1162 |
| Lsp1109I | GCAGC | 4 cut(s) | 90, 556, 745, 992 |
| MaeI | CTAG | 3 cut(s) | 689, 1217, 1239 |
| MaeIII | GTNAC | 3 cut(s) | 353, 1133, 1276 |
| MalI | GATC | 6 cut(s) | 52, 306, 504, 853, 948, 1105 |
| MboI | GATC | 6 cut(s) | 50, 304, 502, 851, 946, 1103 |
| MboII | GAAGA | 7 cut(s) | 108, 173, 512, 581, 1103, 1257, 1260 |
| MflI | RGATCY | 1 cut(s) | 946 |
| MhlI | GDGCHC | 1 cut(s) | 402 |
| MlyI | GAGTC | 6 cut(s) | 302, 1057, 1079, 1139, 1190, 1195 |
| MnlI | CCTC | 9 cut(s) | 198, 443, 687, 691, 924, 960, 1153, 1186, 1260 |
| MroNI | GCCGGC | 1 cut(s) | 867 |
| MroXI | GAANNNNTTC | 1 cut(s) | 8 |
| MseI | TTAA | 9 cut(s) | 21, 111, 179, 237, 282, 324, 513, 663, 893 |
| MslI | CAYNNNNRTG | 2 cut(s) | 720, 1259 |
| MspCI | CTTAAG | 1 cut(s) | 236 |
| MspI | CCGG | 2 cut(s) | 868, 1036 |
| MspR9I | CCNGG | 1 cut(s) | 1208 |
| MvaI | CCWGG | 1 cut(s) | 1208 |
| MvnI | CGCG | 2 cut(s) | 139, 141 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 1001 |
| NaeI | GCCGGC | 1 cut(s) | 869 |
| NdeI | CATATG | 1 cut(s) | 748 |
| NdeII | GATC | 6 cut(s) | 50, 304, 502, 851, 946, 1103 |
| NgoMIV | GCCGGC | 1 cut(s) | 867 |
| NheI | GCTAGC | 1 cut(s) | 688 |
| NlaIII | CATG | 4 cut(s) | 596, 725, 1229, 1258 |
| NlaIV | GGNNCC | 2 cut(s) | 702, 1174 |
| NmuCI | GTSAC | 3 cut(s) | 353, 1133, 1276 |
| NspI | RCATGY | 1 cut(s) | 1229 |
| PagI | TCATGA | 2 cut(s) | 592, 1254 |
| PdiI | GCCGGC | 1 cut(s) | 869 |
| PdmI | GAANNNNTTC | 1 cut(s) | 8 |
| PfeI | GAWTC | 3 cut(s) | 406, 1121, 1251 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 710 |
| PkrI | GCNGC | 4 cut(s) | 80, 571, 760, 982 |
| PleI | GAGTC | 6 cut(s) | 301, 1056, 1079, 1139, 1189, 1195 |
| PpsI | GAGTC | 6 cut(s) | 301, 1056, 1079, 1139, 1189, 1195 |
| Psp6I | CCWGG | 1 cut(s) | 1206 |
| PspGI | CCWGG | 1 cut(s) | 1206 |
| PspN4I | GGNNCC | 2 cut(s) | 702, 1174 |
| PspPI | GGNCC | 3 cut(s) | 701, 781, 1173 |
| PstI | CTGCAG | 1 cut(s) | 736 |
| PstNI | CAGNNNCTG | 2 cut(s) | 59, 98 |
| PsuI | RGATCY | 1 cut(s) | 946 |
| RseI | CAYNNNNRTG | 2 cut(s) | 720, 1259 |
| SaqAI | TTAA | 9 cut(s) | 21, 111, 179, 237, 282, 324, 513, 663, 893 |
| SatI | GCNGC | 4 cut(s) | 79, 570, 759, 981 |
| Sau3AI | GATC | 6 cut(s) | 50, 304, 502, 851, 946, 1103 |
| Sau96I | GGNCC | 3 cut(s) | 701, 781, 1173 |
| SchI | GAGTC | 6 cut(s) | 302, 1057, 1079, 1139, 1190, 1195 |
| ScrFI | CCNGG | 1 cut(s) | 1208 |
| SduI | GDGCHC | 1 cut(s) | 402 |
| SfcI | CTRYAG | 1 cut(s) | 732 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 720, 1259 |
| SmlI | CTYRAG | 2 cut(s) | 236, 573 |
| SmoI | CTYRAG | 2 cut(s) | 236, 573 |
| SsiI | CCGC | 2 cut(s) | 137, 141 |
| SspMI | CTAG | 3 cut(s) | 689, 1217, 1239 |
| StyD4I | CCNGG | 1 cut(s) | 1206 |
| StyI | CCWWGG | 2 cut(s) | 297, 536 |
| TaaI | ACNGT | 2 cut(s) | 431, 909 |
| TaqI | TCGA | 2 cut(s) | 622, 952 |
| TfiI | GAWTC | 3 cut(s) | 406, 1121, 1251 |
| Tru1I | TTAA | 9 cut(s) | 21, 111, 179, 237, 282, 324, 513, 663, 893 |
| Tru9I | TTAA | 9 cut(s) | 21, 111, 179, 237, 282, 324, 513, 663, 893 |
| TscAI | CASTG | 1 cut(s) | 982 |
| TseFI | GTSAC | 3 cut(s) | 353, 1133, 1276 |
| TseI | GCWGC | 4 cut(s) | 78, 569, 758, 980 |
| Tsp45I | GTSAC | 3 cut(s) | 353, 1133, 1276 |
| TspDTI | ATGAA | 7 cut(s) | 17, 174, 489, 581, 609, 846, 1243 |
| TspRI | CASTG | 1 cut(s) | 982 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 710 |
| Vha464I | CTTAAG | 1 cut(s) | 236 |
| XapI | RAATTY | 4 cut(s) | 130, 453, 658, 834 |
| XceI | RCATGY | 1 cut(s) | 1229 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 534, 1145 |
| XmiI | GTMKAC | 1 cut(s) | 145 |
| XmnI | GAANNNNTTC | 1 cut(s) | 8 |
| XspI | CTAG | 3 cut(s) | 689, 1217, 1239 |