RchiOBHm_Chr1g0350341

vestitone reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000382
Physical Location & Seq
Forward (+)
43486841 .. 43494992
8152 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57621

Sequence Viewer

Length: 1839 bp
ATGGAAGAAGTCAGAGGCACAGTTTGTGTAACTGGTGGAACAGGATATATAGCCTCATGGCTGATTCTGAGGCTGCTTCAGCATGGTTACTCGGTTCGAGCCACCATTAGATCCAACCCTGCTGAAGGCAAGAGAGATATCAGCTACCTGACAAGCCTTCCAGGAGCACAAGAGAAACTCCAAATCTTCCATGCAGATCTCAACAACCCCGAGACCTTCAACGACGCCATCCAAGGCTGCACCGGTGTCTTCCACCTTGCTCACCCCATGGACGTTGAAGGCAAAGAACCCGAGGAAACCGTTACCAAACGAGCCCTGGAAGGAACACTAGGCATTTTGAAGGCATGCCTATGTTGCAAGACCGTGAGACGAGTGGTTTACACATCCAGTCTGGCCACAATATTGTACAACAGCAAGCGTCTTGAAGAAACTGATGAAAACACATGGAGTGAGGTTGATGTATGCAGAAGTAACAAGCTTGTGAGCTCTTCTTACTTGGTTTCCAAGACTCTGGTGGAGAGGTCAGCCCTGGAATTTGCAGTGAGGAATGGTTTGGATCTTGTCACTGTGGTTTTGCCTATAGTAGTTGGACCCTTCGTTTGTCCAAATGTTCCTGCTTCCGTGTACATGGGCATGGCTCCCATTTTTGGTGACCAAGAGCAATGTAAATATCTTGTTAGCACATATATGGTGCACATAGATGATGTGGCGAGTGCCCATATCTTCCTTCTTGAACATGCTAATGCAAAAGGGAGGTACATTTGTTCGTCGATCCAAACAACGATTCATGAGTTATATGAGTTTCTTTGCGCAAGATACCCAGAATTTCATATATCAATACCAGATCAGTTAATGGAGATTAAAGGTTACAAAGGAACTAATGTGTCATCAACGAAACTGTTGAATACTGGCTTCAAATTTAAATGCGGCCTTGGGGAAATGTTTGATGGCTCTATTCAATCTTGCAAAGAGAAGGAACACAAGAAAGATTTAAGCTTCCTCACAGCTCTACCAGGAGCAGAAGAAAGGCTCCAAATATTCAACGCGGACCTAAGCAACCCGGAAAGTTTCAATGCAGCCATCCAAGGATGCGTCGGAGTGTTCCATGTTGCTACTCCGGTTGACTTCCAAGACAAGGAACCTGAACCAGTAGTGACCAAACGATCAGTTGATGGAGCCCTTGGTATCCTCAAGGCATGCCTATATGCAAAAACAGTGAAACGAGTCGTGTACACTTCTAGTGAGTCTGTTGTTGCATTTAACAACAAGGATGTGGAAGACATGGATGAGAGTTTTTGGAGTGACACAGATTACATTAAAGCTTTAAAGTCGTATGGAGGCTCATATGCGATTTCGAAGACATTGACTGAAAGAGCAGTTCTTGAATTTTCGGAGAAAAATGGATTGGATGTTGTGACGGTAATTCCTTCTGTTGTTGTTGGCCCCTTCATTTGTCCCAAGTTCGCCGGCTCTGTTCGAAGCGCATTGGCTCTGGTTTTGGGTAACAAGGATGAATATCCTTTTCTTATCAATTTATCAGTGGTACATGTGGATGATGTCGCCAGAGCGCACATTTTCCTTTTCGAACATCATGATGCAAAAGGGAGATACAATTCCTCATCAAATGTCATAACAATTGAAAAAATGGCCAAGTTCCTTTCTGCCAAATTCCCCGAATTTCAAATACCCTCAGCAGAGTCATTAAATGAAGTTAAAGGTCCTAAGTTTCCTGGTCTGTCATCAAAGAAACTCTTGGATTCGGGTTTCAAATTTAAGTATGGGGTTGATGAGATGTTCAGTGAGGCAATTCACTGCTGCAAAGAGAAGGGTTATCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00943 ko01110 map00943 map01110
Pfam Domains
Protein Families
Epimerase

Protein Analysis

612

Amino Acids

67.66

Weight (kDa)

6.02

Isoelectric Point (pI)

38.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 92 1.1e-07 NmrA-like family
Epimerase PF01370 8 - 250 1.5e-21 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 192 7.8e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 10 - 197 2.6e-09 Male sterility protein
GDP_Man_Dehyd PF16363 10 - 267 2.2e-12 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 12 - 133 2.6e-06 NAD(P)H-binding
NAD_binding_4 PF07993 339 - 486 3.2e-09 Male sterility protein
Epimerase PF01370 340 - 538 2.9e-17 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 346 - 526 1.5e-12 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45400 AT2G45400 AT2G45400 AT2G45400
fragaria_vesca FvH4_7g11750 FvH4_7g11760 FvH4_7g11780 FvH4_7g11781 FvH4_7g11781 FvH4_7g11790 FvH4_7g11790
malus_domestica MD02G1205000.v1.1 MD07G1121700.v1.1 MD08G1231300.v1.1 MD08G1231400.v1.1 MD08G1231500.v1.1
prunus_persica Prupe.2G144600_v2.0.a1 Prupe.2G144700_v2.0.a1 Prupe.2G144800_v2.0.a1
pyrus_communis pycom08g20120
rosa_chinensis RchiOBHm_Chr1g0350271 RchiOBHm_Chr1g0350281 RchiOBHm_Chr1g0350311 RchiOBHm_Chr1g0350321 RchiOBHm_Chr1g0350341 RchiOBHm_Chr1g0350361 RchiOBHm_Chr1g0350371 RchiOBHm_Chr1g0350401 RchiOBHm_Chr1g0350411 RchiOBHm_Chr1g0350431 RchiOBHm_Chr1g0350441 RchiOBHm_Chr1g0350471
rosa_laevigata RLG00000028483 RLG00000028485 RLG00000028486 RLG00000028487 RLG00000028488 RLG00000028489 RLG00000028493 RLG00000028494 RLG00000035411
rosa_multiflora Rmu_co8173820.1_g000001 Rmu_sc0001037.1_g000006 Rmu_sc0001864.1_g000002 Rmu_sc0001864.1_g000009 Rmu_ssc0000083.1_g000008 Rmu_ssc0000083.1_g000013 Rmu_ssc0000083.1_g000018
rosa_roxburghii Rroxscaffold_4G00304840 Rroxscaffold_4G00304860 Rroxscaffold_4G00304890 Rroxscaffold_4G00304900 Rroxscaffold_4G00304920 Rroxscaffold_4G00304930
rosa_rugosa Rorug01G0210800 Rorug01G0210900 Rorug01G0211000 Rorug01G0211100 Rorug01G0211200 Rorug01G0211300 Rorug01G0211300 Rorug01G0211400
rosa_samantha Rh1AG225500 Rh1AG225600 Rh1AG225700 Rh1AG225800 Rh1AG225900 Rh1AG226000 Rh1BG193400 Rh1BG193600 Rh1BG193700 Rh1BG193800 Rh1BG193900 Rh1BG194000 Rh1BG194200 Rh1CG210300 Rh1CG210500 Rh1CG210600 Rh1CG210700 Rh1CG210900 Rh1DG221700 Rh1DG221900 Rh1DG222100 Rh1DG222200 Rh1DG222300 Rh1DG222400 Rh1DG222500 Rh2BG259100 Rh3DG280100 Rh5AG528000
rosa_wichuraiana Rw0G018730 Rw0G018740 Rw0G018750 Rw0G018760 Rw0G018770 Rw0G018790 Rw1G019390 Rw1G019410 Rw1G019420 Rw1G019430 Rw1G019440 Rw1G019450 Rw1G019480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 811
AccII CGCG 1 cut(s) 1046
AciI CCGC 2 cut(s) 927, 1046
AclWI GGATC 3 cut(s) 105, 564, 766
AcoI YGGCCR 2 cut(s) 393, 1647
AcsI RAATTY 7 cut(s) 533, 824, 917, 1385, 1667, 1676, 1769
AcuI CTGAAG 2 cut(s) 62, 144
AcyI GRCGYC 1 cut(s) 225
AfaI GTAC 5 cut(s) 407, 626, 758, 1232, 1545
AfiI CCNNNNNNNGG 3 cut(s) 125, 647, 1135
AflIII ACRYGT 1 cut(s) 1546
AgeI ACCGGT 1 cut(s) 242
AjnI CCWGG 5 cut(s) 160, 315, 528, 1012, 1729
AjuI GAANNNNNNNTTGG 4 cut(s) 768, 800, 1388, 1420
AluBI AGCT 6 cut(s) 144, 478, 486, 996, 1007, 1322
AluI AGCT 6 cut(s) 144, 478, 486, 996, 1007, 1322
Alw21I GWGCWC 3 cut(s) 169, 488, 696
Alw26I GTCTC 2 cut(s) 206, 361
Alw44I GTGCAC 1 cut(s) 692
AlwI GGATC 3 cut(s) 105, 564, 766
Ama87I CYCGRG 2 cut(s) 209, 290
AoxI GGCC 4 cut(s) 393, 928, 1441, 1647
ApaLI GTGCAC 1 cut(s) 692
ApeKI GCWGC 4 cut(s) 73, 237, 1076, 1815
ApoI RAATTY 7 cut(s) 533, 824, 917, 1385, 1667, 1676, 1769
ArsI GACNNNNNNTTYG 2 cut(s) 582, 614
AsiGI ACCGGT 1 cut(s) 242
AspLEI GCGC 3 cut(s) 812, 1484, 1570
AspS9I GGNCC 4 cut(s) 590, 1048, 1442, 1718
AsuC2I CCSGG 1 cut(s) 1061
AsuHPI GGTGA 2 cut(s) 254, 662
AsuII TTCGAA 3 cut(s) 1355, 1477, 1584
AvaI CYCGRG 2 cut(s) 209, 290
AvaII GGWCC 3 cut(s) 590, 1048, 1718
BaeGI GKGCMC 2 cut(s) 696, 718
BaeI ACNNNNGTAYC 2 cut(s) 748, 781
BalI TGGCCA 2 cut(s) 395, 1649
BanII GRGCYC 3 cut(s) 316, 488, 1180
BbsI GAAGAC 3 cut(s) 241, 1284, 1364
Bbv12I GWGCWC 3 cut(s) 169, 488, 696
BbvCI CCTCAGC 1 cut(s) 1690
BbvI GCAGC 4 cut(s) 60, 224, 1088, 1802
BccI CCATC 4 cut(s) 236, 941, 1088, 1166
BciT130I CCWGG 5 cut(s) 162, 317, 530, 1014, 1731
BciVI GTATCC 1 cut(s) 1196
BcnI CCSGG 1 cut(s) 1061
BcoDI GTCTC 2 cut(s) 206, 361
BfaI CTAG 2 cut(s) 329, 1239
BfmI CTRYAG 1 cut(s) 579
BfuI GTATCC 1 cut(s) 1196
BglII AGATCT 1 cut(s) 196
BisI GCNGC 5 cut(s) 74, 238, 928, 1077, 1816
BlsI GCNGC 5 cut(s) 75, 239, 929, 1078, 1817
Bme1390I CCNGG 6 cut(s) 162, 317, 530, 1014, 1061, 1731
Bme18I GGWCC 3 cut(s) 590, 1048, 1718
BmeT110I CYCGRG 2 cut(s) 209, 290
BmgT120I GGNCC 4 cut(s) 590, 1048, 1442, 1718
BmiI GGNNCC 6 cut(s) 592, 639, 1031, 1140, 1177, 1444
BmrFI CCNGG 6 cut(s) 162, 317, 530, 1014, 1061, 1731
BmsI GCATC 2 cut(s) 1079, 1585
BpiI GAAGAC 3 cut(s) 241, 1284, 1364
Bpu10I CCTNAGC 2 cut(s) 1052, 1690
Bpu14I TTCGAA 3 cut(s) 1355, 1477, 1584
BpuEI CTTGAG 1 cut(s) 1175
BpuMI CCSGG 1 cut(s) 1061
BsaBI GATNNNNATC 1 cut(s) 1515
BsaHI GRCGYC 1 cut(s) 225
BsaI GGTCTC 1 cut(s) 206
BsaJI CCNNGG 8 cut(s) 232, 267, 291, 315, 528, 931, 1084, 1180
BsaWI WCCGGW 2 cut(s) 242, 1117
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 647, 1135
Bse118I RCCGGY 2 cut(s) 242, 1466
Bse1I ACTGG 4 cut(s) 37, 387, 913, 1148
Bse3DI GCAATG 1 cut(s) 668
Bse8I GATNNNNATC 1 cut(s) 1515
BseBI CCWGG 5 cut(s) 162, 317, 530, 1014, 1731
BseDI CCNNGG 8 cut(s) 232, 267, 291, 315, 528, 931, 1084, 1180
BseGI GGATG 9 cut(s) 228, 383, 1080, 1094, 1276, 1291, 1414, 1516, 1558
BseJI GATNNNNATC 1 cut(s) 1515
BseLI CCNNNNNNNGG 3 cut(s) 125, 647, 1135
BseMI GCAATG 1 cut(s) 668
BseMII CTCAG 2 cut(s) 59, 1704
BseNI ACTGG 4 cut(s) 37, 387, 913, 1148
BseSI GKGCMC 2 cut(s) 696, 718
BseXI GCAGC 4 cut(s) 60, 224, 1088, 1802
BsgI GTGCAG 1 cut(s) 223
Bsh1236I CGCG 1 cut(s) 1046
BshFI GGCC 4 cut(s) 395, 930, 1443, 1649
BshTI ACCGGT 1 cut(s) 242
BsiHKAI GWGCWC 3 cut(s) 169, 488, 696
BsiHKCI CYCGRG 2 cut(s) 209, 290
BsiSI CCGG 4 cut(s) 243, 1061, 1118, 1467
BslFI GGGAC 1 cut(s) 1440
BslI CCNNNNNNNGG 3 cut(s) 125, 647, 1135
BsmAI GTCTC 2 cut(s) 206, 361
BsmBI CGTCTC 1 cut(s) 361
BsmFI GGGAC 1 cut(s) 1440
BsnI GGCC 4 cut(s) 395, 930, 1443, 1649
Bso31I GGTCTC 1 cut(s) 206
BsoBI CYCGRG 2 cut(s) 209, 290
Bsp119I TTCGAA 3 cut(s) 1355, 1477, 1584
Bsp1286I GDGCHC 6 cut(s) 169, 316, 488, 696, 718, 1180
Bsp1407I TGTACA 3 cut(s) 405, 624, 1230
Bsp143I GATC 6 cut(s) 110, 196, 556, 771, 844, 1163
Bsp19I CCATGG 1 cut(s) 267
BspACI CCGC 2 cut(s) 927, 1046
BspANI GGCC 4 cut(s) 395, 930, 1443, 1649
BspCNI CTCAG 2 cut(s) 60, 1703
BspFNI CGCG 1 cut(s) 1046
BspHI TCATGA 2 cut(s) 787, 1591
BspLI GGNNCC 6 cut(s) 592, 639, 1031, 1140, 1177, 1444
BspPI GGATC 3 cut(s) 105, 564, 766
BspQI GCTCTTC 1 cut(s) 493
BspT104I TTCGAA 3 cut(s) 1355, 1477, 1584
BspTNI GGTCTC 1 cut(s) 206
BsrDI GCAATG 1 cut(s) 668
BsrFI RCCGGY 2 cut(s) 242, 1466
BsrGI TGTACA 3 cut(s) 405, 624, 1230
BsrI ACTGG 4 cut(s) 37, 387, 913, 1148
BssAI RCCGGY 2 cut(s) 242, 1466
BssECI CCNNGG 8 cut(s) 232, 267, 291, 315, 528, 931, 1084, 1180
BssMI GATC 6 cut(s) 110, 196, 556, 771, 844, 1163
BssNI GRCGYC 1 cut(s) 225
BssT1I CCWWGG 5 cut(s) 232, 267, 931, 1084, 1180
Bst2UI CCWGG 5 cut(s) 162, 317, 530, 1014, 1731
Bst4CI ACNGT 7 cut(s) 22, 301, 364, 568, 900, 1216, 1420
Bst6I CTCTTC 1 cut(s) 493
BstACI GRCGYC 1 cut(s) 225
BstAUI TGTACA 3 cut(s) 405, 624, 1230
BstBI TTCGAA 3 cut(s) 1355, 1477, 1584
BstC8I GCNNGC 4 cut(s) 346, 416, 1198, 1468
BstDEI CTNAG 4 cut(s) 68, 1052, 1690, 1722
BstDSI CCRYGG 1 cut(s) 267
BstEII GGTNACC 1 cut(s) 650
BstENI CCTNNNNNAGG 1 cut(s) 123
BstF5I GGATG 9 cut(s) 228, 383, 1080, 1094, 1276, 1291, 1414, 1516, 1558
BstFNI CGCG 1 cut(s) 1046
BstHHI GCGC 3 cut(s) 812, 1484, 1570
BstKTI GATC 6 cut(s) 113, 199, 559, 774, 847, 1166
BstMAI GTCTC 2 cut(s) 206, 361
BstMBI GATC 6 cut(s) 110, 196, 556, 771, 844, 1163
BstMWI GCNNNNNNNGC 2 cut(s) 79, 354
BstNI CCWGG 5 cut(s) 162, 317, 530, 1014, 1731
BstNSI RCATGY 4 cut(s) 348, 740, 1200, 1550
BstPI GGTNACC 1 cut(s) 650
BstSCI CCNGG 6 cut(s) 160, 315, 528, 1012, 1059, 1729
BstSFI CTRYAG 1 cut(s) 579
BstSLI GKGCMC 2 cut(s) 696, 718
BstUI CGCG 1 cut(s) 1046
BstV1I GCAGC 4 cut(s) 60, 224, 1088, 1802
BstV2I GAAGAC 3 cut(s) 241, 1284, 1364
BstX2I RGATCY 3 cut(s) 110, 196, 556
BstXI CCANNNNNNTGG 1 cut(s) 511
BstYI RGATCY 3 cut(s) 110, 196, 556
BsuI GTATCC 1 cut(s) 1196
BsuRI GGCC 4 cut(s) 395, 930, 1443, 1649
BtgI CCRYGG 1 cut(s) 267
BtsCI GGATG 9 cut(s) 228, 383, 1080, 1094, 1276, 1291, 1414, 1516, 1558
BtsI GCAGTG 2 cut(s) 546, 1810
BtsIMutI CAGTG 6 cut(s) 546, 564, 1221, 1545, 1804, 1810
Cac8I GCNNGC 4 cut(s) 346, 416, 1198, 1468
CciI TCATGA 2 cut(s) 787, 1591
CfoI GCGC 3 cut(s) 812, 1484, 1570
Cfr10I RCCGGY 2 cut(s) 242, 1466
Cfr13I GGNCC 4 cut(s) 590, 1048, 1442, 1718
CseI GACGC 3 cut(s) 233, 407, 1081
Csp6I GTAC 5 cut(s) 406, 625, 757, 1231, 1544
CspAI ACCGGT 1 cut(s) 242
CviQI GTAC 5 cut(s) 406, 625, 757, 1231, 1544
DdeI CTNAG 4 cut(s) 68, 1052, 1690, 1722
DpnI GATC 6 cut(s) 112, 198, 558, 773, 846, 1165
DpnII GATC 6 cut(s) 110, 196, 556, 771, 844, 1163
DraI TTTAAA 2 cut(s) 922, 1326
EaeI YGGCCR 2 cut(s) 393, 1647
Eam1104I CTCTTC 1 cut(s) 493
EarI CTCTTC 1 cut(s) 493
Ecl136II GAGCTC 1 cut(s) 486
Eco130I CCWWGG 5 cut(s) 232, 267, 931, 1084, 1180
Eco24I GRGCYC 3 cut(s) 316, 488, 1180
Eco31I GGTCTC 1 cut(s) 206
Eco32I GATATC 1 cut(s) 139
Eco47I GGWCC 3 cut(s) 590, 1048, 1718
Eco53kI GAGCTC 1 cut(s) 486
Eco57I CTGAAG 2 cut(s) 62, 144
Eco88I CYCGRG 2 cut(s) 209, 290
Eco91I GGTNACC 1 cut(s) 650
EcoICRI GAGCTC 1 cut(s) 486
EcoNI CCTNNNNNAGG 1 cut(s) 123
EcoO109I RGGNCCY 1 cut(s) 1718
EcoO65I GGTNACC 1 cut(s) 650
EcoRII CCWGG 5 cut(s) 160, 315, 528, 1012, 1729
EcoRV GATATC 1 cut(s) 139
EcoT14I CCWWGG 5 cut(s) 232, 267, 931, 1084, 1180
EcoT38I GRGCYC 3 cut(s) 316, 488, 1180
ErhI CCWWGG 5 cut(s) 232, 267, 931, 1084, 1180
Esp3I CGTCTC 1 cut(s) 361
FalI AAGNNNNNCTT 2 cut(s) 1736, 1768
FaqI GGGAC 1 cut(s) 1440
FauNDI CATATG 1 cut(s) 1345
Fnu4HI GCNGC 5 cut(s) 74, 238, 928, 1077, 1816
FokI GGATG 9 cut(s) 215, 370, 1067, 1101, 1283, 1298, 1421, 1523, 1565
FriOI GRGCYC 3 cut(s) 316, 488, 1180
Fsp4HI GCNGC 5 cut(s) 74, 238, 928, 1077, 1816
FspBI CTAG 2 cut(s) 329, 1239
FspI TGCGCA 1 cut(s) 811
GlaI GCGC 3 cut(s) 811, 1483, 1569
GluI GCNGC 5 cut(s) 74, 238, 928, 1077, 1816
HaeIII GGCC 4 cut(s) 395, 930, 1443, 1649
HapII CCGG 4 cut(s) 243, 1061, 1118, 1467
HgaI GACGC 3 cut(s) 233, 407, 1081
HhaI GCGC 3 cut(s) 812, 1484, 1570
Hin1I GRCGYC 1 cut(s) 225
Hin6I GCGC 3 cut(s) 810, 1482, 1568
HinP1I GCGC 3 cut(s) 810, 1482, 1568
HincII GTYRAC 1 cut(s) 1123
HindII GTYRAC 1 cut(s) 1123
HindIII AAGCTT 3 cut(s) 476, 994, 1320
HinfI GANTC 7 cut(s) 64, 508, 784, 1224, 1244, 1697, 1757
HpaII CCGG 4 cut(s) 243, 1061, 1118, 1467
HphI GGTGA 2 cut(s) 254, 662
Hpy166II GTNNAC 6 cut(s) 379, 625, 694, 1123, 1231, 1233
Hpy188I TCNGA 4 cut(s) 14, 69, 1097, 1393
Hpy188III TCNNGA 5 cut(s) 422, 731, 788, 1382, 1592
Hpy8I GTNNAC 6 cut(s) 379, 625, 694, 1123, 1231, 1233
Hpy99I CGWCG 3 cut(s) 227, 772, 1097
HpyCH4III ACNGT 7 cut(s) 22, 301, 364, 568, 900, 1216, 1420
HpyCH4IV ACGT 1 cut(s) 273
HpyF10VI GCNNNNNNNGC 2 cut(s) 79, 354
HpyF3I CTNAG 4 cut(s) 68, 1052, 1690, 1722
HpySE526I ACGT 1 cut(s) 273
Hsp92I GRCGYC 1 cut(s) 225
HspAI GCGC 3 cut(s) 810, 1482, 1568
KroI GCCGGC 1 cut(s) 1466
KroNI GCCGGC 1 cut(s) 1468
Kzo9I GATC 6 cut(s) 110, 196, 556, 771, 844, 1163
LguI GCTCTTC 1 cut(s) 493
LmnI GCTCC 5 cut(s) 164, 643, 1016, 1035, 1175
Lsp1109I GCAGC 4 cut(s) 60, 224, 1088, 1802
LweI GCATC 2 cut(s) 1079, 1585
MaeI CTAG 2 cut(s) 329, 1239
MaeII ACGT 1 cut(s) 273
MalI GATC 6 cut(s) 112, 198, 558, 773, 846, 1165
MboI GATC 6 cut(s) 110, 196, 556, 771, 844, 1163
MboII GAAGA 9 cut(s) 17, 178, 241, 437, 480, 715, 1034, 1289, 1369
MfeI CAATTG 1 cut(s) 1635
MflI RGATCY 3 cut(s) 110, 196, 556
MhlI GDGCHC 6 cut(s) 169, 316, 488, 696, 718, 1180
MlsI TGGCCA 2 cut(s) 395, 1649
MluNI TGGCCA 2 cut(s) 395, 1649
MlyI GAGTC 4 cut(s) 502, 1233, 1253, 1706
MmeI TCCRAC 3 cut(s) 138, 568, 1075
Mox20I TGGCCA 2 cut(s) 395, 1649
MroNI GCCGGC 1 cut(s) 1466
MscI TGGCCA 2 cut(s) 395, 1649
MslI CAYNNNNRTG 7 cut(s) 349, 632, 686, 699, 741, 1551, 1593
Msp20I TGGCCA 2 cut(s) 395, 1649
MspI CCGG 4 cut(s) 243, 1061, 1118, 1467
MspR9I CCNGG 6 cut(s) 162, 317, 530, 1014, 1061, 1731
MunI CAATTG 1 cut(s) 1635
MvaI CCWGG 5 cut(s) 162, 317, 530, 1014, 1731
MvnI CGCG 1 cut(s) 1046
MwoI GCNNNNNNNGC 2 cut(s) 79, 354
NaeI GCCGGC 1 cut(s) 1468
NciI CCSGG 1 cut(s) 1061
NcoI CCATGG 1 cut(s) 267
NdeI CATATG 1 cut(s) 1345
NdeII GATC 6 cut(s) 110, 196, 556, 771, 844, 1163
NgoMIV GCCGGC 1 cut(s) 1466
NlaIV GGNNCC 6 cut(s) 592, 639, 1031, 1140, 1177, 1444
NmuCI GTSAC 5 cut(s) 562, 650, 1153, 1301, 1414
NsbI TGCGCA 1 cut(s) 811
NspI RCATGY 4 cut(s) 348, 740, 1200, 1550
NspV TTCGAA 3 cut(s) 1355, 1477, 1584
PaeI GCATGC 2 cut(s) 348, 1200
PagI TCATGA 2 cut(s) 787, 1591
PciI ACATGT 1 cut(s) 1546
PciSI GCTCTTC 1 cut(s) 493
PdiI GCCGGC 1 cut(s) 1468
PfeI GAWTC 3 cut(s) 64, 784, 1757
PfoI TCCNGGA 1 cut(s) 160
PinAI ACCGGT 1 cut(s) 242
PkrI GCNGC 5 cut(s) 75, 239, 929, 1078, 1817
PleI GAGTC 4 cut(s) 502, 1232, 1252, 1705
PpsI GAGTC 4 cut(s) 502, 1232, 1252, 1705
PpuMI RGGWCCY 1 cut(s) 1718
PscI ACATGT 1 cut(s) 1546
Psp124BI GAGCTC 1 cut(s) 488
Psp5II RGGWCCY 1 cut(s) 1718
Psp6I CCWGG 5 cut(s) 160, 315, 528, 1012, 1729
PspEI GGTNACC 1 cut(s) 650
PspGI CCWGG 5 cut(s) 160, 315, 528, 1012, 1729
PspN4I GGNNCC 6 cut(s) 592, 639, 1031, 1140, 1177, 1444
PspPI GGNCC 4 cut(s) 590, 1048, 1442, 1718
PspPPI RGGWCCY 1 cut(s) 1718
PsuI RGATCY 3 cut(s) 110, 196, 556
RsaI GTAC 5 cut(s) 407, 626, 758, 1232, 1545
RsaNI GTAC 5 cut(s) 406, 625, 757, 1231, 1544
RseI CAYNNNNRTG 7 cut(s) 349, 632, 686, 699, 741, 1551, 1593
SacI GAGCTC 1 cut(s) 488
SapI GCTCTTC 1 cut(s) 493
SatI GCNGC 5 cut(s) 74, 238, 928, 1077, 1816
Sau3AI GATC 6 cut(s) 110, 196, 556, 771, 844, 1163
Sau96I GGNCC 4 cut(s) 590, 1048, 1442, 1718
SchI GAGTC 4 cut(s) 502, 1233, 1253, 1706
ScrFI CCNGG 6 cut(s) 162, 317, 530, 1014, 1061, 1731
SduI GDGCHC 6 cut(s) 169, 316, 488, 696, 718, 1180
SfaNI GCATC 2 cut(s) 1079, 1585
SfcI CTRYAG 1 cut(s) 579
SfuI TTCGAA 3 cut(s) 1355, 1477, 1584
SgrAI CRCCGGYG 1 cut(s) 242
SinI GGWCC 3 cut(s) 590, 1048, 1718
SmiI ATTTAAAT 1 cut(s) 922
SmiMI CAYNNNNRTG 7 cut(s) 349, 632, 686, 699, 741, 1551, 1593
SmlI CTYRAG 1 cut(s) 1190
SmoI CTYRAG 1 cut(s) 1190
SphI GCATGC 2 cut(s) 348, 1200
SsiI CCGC 2 cut(s) 927, 1046
SspI AATATT 2 cut(s) 402, 1038
SspMI CTAG 2 cut(s) 329, 1239
SstI GAGCTC 1 cut(s) 488
StyD4I CCNGG 6 cut(s) 160, 315, 528, 1012, 1059, 1729
StyI CCWWGG 5 cut(s) 232, 267, 931, 1084, 1180
SwaI ATTTAAAT 1 cut(s) 922
TaaI ACNGT 7 cut(s) 22, 301, 364, 568, 900, 1216, 1420
TaiI ACGT 1 cut(s) 276
TaqI TCGA 5 cut(s) 97, 770, 1355, 1477, 1584
TatI WGTACW 3 cut(s) 405, 624, 1230
TauI GCSGC 1 cut(s) 930
TfiI GAWTC 3 cut(s) 64, 784, 1757
TscAI CASTG 6 cut(s) 546, 571, 1221, 1545, 1804, 1817
TseFI GTSAC 5 cut(s) 562, 650, 1153, 1301, 1414
TseI GCWGC 4 cut(s) 73, 237, 1076, 1815
Tsp45I GTSAC 5 cut(s) 562, 650, 1153, 1301, 1414
TspDTI ATGAA 6 cut(s) 450, 776, 818, 1438, 1527, 1722
TspGWI ACGGA 1 cut(s) 610
TspRI CASTG 6 cut(s) 546, 571, 1221, 1545, 1804, 1817
VneI GTGCAC 1 cut(s) 692
VpaK11BI GGWCC 3 cut(s) 590, 1048, 1718
XagI CCTNNNNNAGG 1 cut(s) 123
XapI RAATTY 7 cut(s) 533, 824, 917, 1385, 1667, 1676, 1769
XceI RCATGY 4 cut(s) 348, 740, 1200, 1550
XcmI CCANNNNNNNNNTGG 2 cut(s) 313, 511
XspI CTAG 2 cut(s) 329, 1239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.