Rh1DG222200

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000382
Physical Location & Seq
Forward (+)
43198294 .. 43215226
16933 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG222200.1

Sequence Viewer

Length: 1194 bp
ATGGAAGGAGAAAAAGGTAGAGTGTGTGTAACGGGAGGTTCAGGGTTCATAGGGTCCTGGTTGATTTTGAGGCTTCTTGAGCATGGTTACTATGTCAATACCACTGTTAGATCAGACCCAGAACACAAGAAAGATTTAAGCTTCCTCACAGCTCTACCAGGAGCAGAAGAAAGGCTCCAAATATTCAACGCATACCTAAGCAATAACCCGGAAAGTTTCAATGCAGCCATCCAAGGATGCGTCGGAGTGTTCCATGTTGCTACTCCGGTTGACTTCCAAGACAAGGAACCTGAACCAGTAGTGACCAAACGATCAGTCGATGGAGCCCTTGGTATCCTCAAGGCGTGCCTATATGCAAAAACAGTGAAACGGGTTGTGTACACTTCTAGTGCCTCTGCTGTTGTATTTAACAACAAGGATGTGGAAGACATGGATGAGAGTTTTTGGAGTGACACAGATTACATTAAAGCTTTAAACCCGTATGGAGGCTCATACTCGATTTCGAAGACATTGACTGAAAGAGCAGTTCTTGAATTTTCGGAGAAAAATGGATTGGATGTTGTGACGGTAATTCCTTCTGTTGTTGTTGGCCCCTTCCTTTGTCCCAAGTTCCCTGGCTCTGTTCGAAGAACAGTGGCTCTGGTTTTGGGTAACAGGGATGAGTATCCTTTTCTTATCAATTTATCAGTGGTACATGTGGATGATGTCGCCAGAGCGCACATTTTCCTTTTCGAACATCATGATGCAAAAGGGAGATACAATTGCTCATCGAATGTCATAACAATTGAAGAAATGGCCAAGTTCCTTTCAGCCAAATTCCCCGAATTTCAAATACCCTCAGCAGAGTCATTGAAGGAAGTTAAAGGTCCTAAGATTCCTGGTCTGTCATCAAAGAAACTCTTAGATACGGGTTTCAAATTTAAGTATGGGGTTGATGAGATGTTTAGTGAGGCAATTCACTGCTGCAAAGAGAAGGACTCTGAATCCTCTTCCCATGGCGGAGTCTCGGAGTACAAGTGTTTCCGTCAGTTTAGCTGTGACCTGATGGAGAGAGATCGGTCGATGGAAGAAGTCAGAGGCACAGTTTGTGTAACTGGTGGAACAGGATATATAGCCTCATGGCTGATTCTGAGGCTGCTTCAGCATGGTTACTCGGTTCGAGCCACCATTAGATCCAACCCTGCTAGTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00943 ko01110 map00943 map01110
Pfam Domains
Protein Families
Epimerase

Protein Analysis

397

Amino Acids

43.95

Weight (kDa)

6.39

Isoelectric Point (pI)

36.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 8 - 255 1.7e-23 NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd PF16363 10 - 268 1.4e-14 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 10 - 242 9e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 10 - 209 7.3e-12 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45400 AT2G45400 AT2G45400 AT2G45400
fragaria_vesca FvH4_7g11750 FvH4_7g11760 FvH4_7g11780 FvH4_7g11781 FvH4_7g11781 FvH4_7g11790 FvH4_7g11790
malus_domestica MD02G1205000.v1.1 MD07G1121700.v1.1 MD08G1231300.v1.1 MD08G1231400.v1.1 MD08G1231500.v1.1
prunus_persica Prupe.2G144600_v2.0.a1 Prupe.2G144700_v2.0.a1 Prupe.2G144800_v2.0.a1
pyrus_communis pycom08g20120
rosa_chinensis RchiOBHm_Chr1g0350271 RchiOBHm_Chr1g0350281 RchiOBHm_Chr1g0350311 RchiOBHm_Chr1g0350321 RchiOBHm_Chr1g0350341 RchiOBHm_Chr1g0350361 RchiOBHm_Chr1g0350371 RchiOBHm_Chr1g0350401 RchiOBHm_Chr1g0350411 RchiOBHm_Chr1g0350431 RchiOBHm_Chr1g0350441 RchiOBHm_Chr1g0350471
rosa_laevigata RLG00000028483 RLG00000028485 RLG00000028486 RLG00000028487 RLG00000028488 RLG00000028489 RLG00000028493 RLG00000028494 RLG00000035411
rosa_multiflora Rmu_co8173820.1_g000001 Rmu_sc0001037.1_g000006 Rmu_sc0001864.1_g000002 Rmu_sc0001864.1_g000009 Rmu_ssc0000083.1_g000008 Rmu_ssc0000083.1_g000013 Rmu_ssc0000083.1_g000018
rosa_roxburghii Rroxscaffold_4G00304840 Rroxscaffold_4G00304860 Rroxscaffold_4G00304890 Rroxscaffold_4G00304900 Rroxscaffold_4G00304920 Rroxscaffold_4G00304930
rosa_rugosa Rorug01G0210800 Rorug01G0210900 Rorug01G0211000 Rorug01G0211100 Rorug01G0211200 Rorug01G0211300 Rorug01G0211300 Rorug01G0211400
rosa_samantha Rh1AG225500 Rh1AG225600 Rh1AG225700 Rh1AG225800 Rh1AG225900 Rh1AG226000 Rh1BG193400 Rh1BG193600 Rh1BG193700 Rh1BG193800 Rh1BG193900 Rh1BG194000 Rh1BG194200 Rh1CG210300 Rh1CG210500 Rh1CG210600 Rh1CG210700 Rh1CG210900 Rh1DG221700 Rh1DG221900 Rh1DG222100 Rh1DG222200 Rh1DG222300 Rh1DG222400 Rh1DG222500 Rh2BG259100 Rh3DG280100 Rh5AG528000
rosa_wichuraiana Rw0G018730 Rw0G018740 Rw0G018750 Rw0G018760 Rw0G018770 Rw0G018790 Rw1G019390 Rw1G019410 Rw1G019420 Rw1G019430 Rw1G019440 Rw1G019450 Rw1G019480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 999
AclWI GGATC 1 cut(s) 1167
AcoI YGGCCR 1 cut(s) 795
AcsI RAATTY 4 cut(s) 533, 815, 824, 917
AcuI CTGAAG 1 cut(s) 1124
AfaI GTAC 3 cut(s) 380, 693, 1013
AfiI CCNNNNNNNGG 2 cut(s) 283, 485
AflIII ACRYGT 1 cut(s) 694
AgsI TTSAA 7 cut(s) 187, 220, 533, 788, 830, 853, 916
AjnI CCWGG 4 cut(s) 56, 157, 613, 877
AjuI GAANNNNNNNTTGG 2 cut(s) 536, 568
AluBI AGCT 4 cut(s) 141, 152, 470, 1035
AluI AGCT 4 cut(s) 141, 152, 470, 1035
Alw26I GTCTC 1 cut(s) 1009
AlwI GGATC 1 cut(s) 1167
AoxI GGCC 2 cut(s) 589, 795
ApeKI GCWGC 3 cut(s) 224, 963, 1135
ApoI RAATTY 4 cut(s) 533, 815, 824, 917
ArsI GACNNNNNNTTYG 2 cut(s) 300, 332
AspLEI GCGC 1 cut(s) 718
AspS9I GGNCC 3 cut(s) 54, 590, 866
AsuC2I CCSGG 1 cut(s) 209
AsuII TTCGAA 3 cut(s) 503, 625, 732
AvaII GGWCC 2 cut(s) 54, 866
BalI TGGCCA 1 cut(s) 797
BanII GRGCYC 1 cut(s) 328
BbsI GAAGAC 2 cut(s) 432, 512
BbvCI CCTCAGC 1 cut(s) 838
BbvI GCAGC 3 cut(s) 236, 950, 1122
BccI CCATC 4 cut(s) 236, 314, 1039, 1057
BciT130I CCWGG 4 cut(s) 58, 159, 615, 879
BciVI GTATCC 2 cut(s) 344, 675
BcnI CCSGG 1 cut(s) 209
BcoDI GTCTC 1 cut(s) 1009
BfaI CTAG 2 cut(s) 387, 1185
BfuI GTATCC 2 cut(s) 344, 675
BisI GCNGC 3 cut(s) 225, 964, 1136
BlsI GCNGC 3 cut(s) 226, 965, 1137
Bme1390I CCNGG 5 cut(s) 58, 159, 209, 615, 879
Bme18I GGWCC 2 cut(s) 54, 866
BmgT120I GGNCC 3 cut(s) 54, 590, 866
BmiI GGNNCC 5 cut(s) 55, 176, 288, 325, 592
BmrFI CCNGG 5 cut(s) 58, 159, 209, 615, 879
BmsI GCATC 2 cut(s) 227, 733
BpiI GAAGAC 2 cut(s) 432, 512
BplI GAGNNNNNCTC 2 cut(s) 962, 994
Bpu10I CCTNAGC 2 cut(s) 197, 838
Bpu14I TTCGAA 3 cut(s) 503, 625, 732
BpuEI CTTGAG 2 cut(s) 98, 323
BpuMI CCSGG 1 cut(s) 209
BsaBI GATNNNNATC 1 cut(s) 663
BsaJI CCNNGG 4 cut(s) 232, 328, 613, 994
BsaWI WCCGGW 1 cut(s) 265
BsaXI ACNNNNNCTCC 2 cut(s) 1001, 1031
Bsc4I CCNNNNNNNGG 2 cut(s) 283, 485
Bse1I ACTGG 2 cut(s) 296, 1099
Bse8I GATNNNNATC 1 cut(s) 663
BseBI CCWGG 4 cut(s) 58, 159, 615, 879
BseDI CCNNGG 4 cut(s) 232, 328, 613, 994
BseGI GGATG 7 cut(s) 228, 242, 424, 439, 562, 664, 706
BseJI GATNNNNATC 1 cut(s) 663
BseLI CCNNNNNNNGG 2 cut(s) 283, 485
BseMII CTCAG 2 cut(s) 852, 1121
BseNI ACTGG 2 cut(s) 296, 1099
BseXI GCAGC 3 cut(s) 236, 950, 1122
Bsh1285I CGRYCG 1 cut(s) 1061
BshFI GGCC 2 cut(s) 591, 797
BsiEI CGRYCG 1 cut(s) 1061
BsiSI CCGG 2 cut(s) 209, 266
BslFI GGGAC 1 cut(s) 588
BslI CCNNNNNNNGG 2 cut(s) 283, 485
BsmAI GTCTC 1 cut(s) 1009
BsmFI GGGAC 1 cut(s) 588
BsnI GGCC 2 cut(s) 591, 797
Bsp119I TTCGAA 3 cut(s) 503, 625, 732
Bsp1286I GDGCHC 1 cut(s) 328
Bsp1407I TGTACA 1 cut(s) 378
Bsp143I GATC 4 cut(s) 110, 311, 1054, 1172
Bsp19I CCATGG 1 cut(s) 994
BspACI CCGC 1 cut(s) 999
BspANI GGCC 2 cut(s) 591, 797
BspCNI CTCAG 2 cut(s) 851, 1122
BspHI TCATGA 1 cut(s) 739
BspLI GGNNCC 5 cut(s) 55, 176, 288, 325, 592
BspPI GGATC 1 cut(s) 1167
BspT104I TTCGAA 3 cut(s) 503, 625, 732
BsrGI TGTACA 1 cut(s) 378
BsrI ACTGG 2 cut(s) 296, 1099
BssECI CCNNGG 4 cut(s) 232, 328, 613, 994
BssMI GATC 4 cut(s) 110, 311, 1054, 1172
BssT1I CCWWGG 3 cut(s) 232, 328, 994
Bst2UI CCWGG 4 cut(s) 58, 159, 615, 879
Bst4CI ACNGT 5 cut(s) 106, 364, 568, 634, 1084
Bst6I CTCTTC 1 cut(s) 994
BstAUI TGTACA 1 cut(s) 378
BstBI TTCGAA 3 cut(s) 503, 625, 732
BstC8I GCNNGC 1 cut(s) 346
BstDEI CTNAG 5 cut(s) 197, 838, 870, 901, 1130
BstDSI CCRYGG 1 cut(s) 994
BstF5I GGATG 7 cut(s) 228, 242, 424, 439, 562, 664, 706
BstHHI GCGC 1 cut(s) 718
BstKTI GATC 4 cut(s) 113, 314, 1057, 1175
BstMAI GTCTC 1 cut(s) 1009
BstMBI GATC 4 cut(s) 110, 311, 1054, 1172
BstMCI CGRYCG 1 cut(s) 1061
BstMWI GCNNNNNNNGC 2 cut(s) 79, 1141
BstNI CCWGG 4 cut(s) 58, 159, 615, 879
BstNSI RCATGY 1 cut(s) 698
BstSCI CCNGG 5 cut(s) 56, 157, 207, 613, 877
BstV1I GCAGC 3 cut(s) 236, 950, 1122
BstV2I GAAGAC 2 cut(s) 432, 512
BstX2I RGATCY 1 cut(s) 1172
BstYI RGATCY 1 cut(s) 1172
BsuI GTATCC 2 cut(s) 344, 675
BsuRI GGCC 2 cut(s) 591, 797
BtgI CCRYGG 1 cut(s) 994
BtsCI GGATG 7 cut(s) 228, 242, 424, 439, 562, 664, 706
BtsI GCAGTG 1 cut(s) 958
BtsIMutI CAGTG 5 cut(s) 102, 369, 639, 693, 958
Cac8I GCNNGC 1 cut(s) 346
CciI TCATGA 1 cut(s) 739
CfoI GCGC 1 cut(s) 718
Cfr13I GGNCC 3 cut(s) 54, 590, 866
CseI GACGC 1 cut(s) 229
Csp6I GTAC 3 cut(s) 379, 692, 1012
CviAII CATG 8 cut(s) 83, 254, 430, 695, 740, 995, 1119, 1145
CviQI GTAC 3 cut(s) 379, 692, 1012
DdeI CTNAG 5 cut(s) 197, 838, 870, 901, 1130
DpnI GATC 4 cut(s) 112, 313, 1056, 1174
DpnII GATC 4 cut(s) 110, 311, 1054, 1172
DraI TTTAAA 1 cut(s) 474
EaeI YGGCCR 1 cut(s) 795
Eam1104I CTCTTC 1 cut(s) 994
EarI CTCTTC 1 cut(s) 994
EciI GGCGGA 1 cut(s) 1014
Eco130I CCWWGG 3 cut(s) 232, 328, 994
Eco24I GRGCYC 1 cut(s) 328
Eco47I GGWCC 2 cut(s) 54, 866
Eco57I CTGAAG 1 cut(s) 1124
EcoO109I RGGNCCY 2 cut(s) 54, 866
EcoRII CCWGG 4 cut(s) 56, 157, 613, 877
EcoT14I CCWWGG 3 cut(s) 232, 328, 994
EcoT38I GRGCYC 1 cut(s) 328
ErhI CCWWGG 3 cut(s) 232, 328, 994
FaeI CATG 8 cut(s) 86, 257, 433, 698, 743, 998, 1122, 1148
FalI AAGNNNNNCTT 2 cut(s) 884, 916
FaqI GGGAC 1 cut(s) 588
FatI CATG 8 cut(s) 82, 253, 429, 694, 739, 994, 1118, 1144
Fnu4HI GCNGC 3 cut(s) 225, 964, 1136
FokI GGATG 7 cut(s) 215, 249, 431, 446, 569, 671, 713
FriOI GRGCYC 1 cut(s) 328
Fsp4HI GCNGC 3 cut(s) 225, 964, 1136
FspBI CTAG 2 cut(s) 387, 1185
GlaI GCGC 1 cut(s) 717
GluI GCNGC 3 cut(s) 225, 964, 1136
HaeIII GGCC 2 cut(s) 591, 797
HapII CCGG 2 cut(s) 209, 266
HgaI GACGC 1 cut(s) 229
HhaI GCGC 1 cut(s) 718
Hin1II CATG 8 cut(s) 86, 257, 433, 698, 743, 998, 1122, 1148
Hin6I GCGC 1 cut(s) 716
HinP1I GCGC 1 cut(s) 716
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HindIII AAGCTT 2 cut(s) 139, 468
HinfI GANTC 6 cut(s) 845, 874, 977, 983, 1002, 1126
HpaII CCGG 2 cut(s) 209, 266
Hpy166II GTNNAC 3 cut(s) 271, 379, 381
Hpy188I TCNGA 7 cut(s) 115, 245, 541, 982, 1009, 1076, 1131
Hpy188III TCNNGA 3 cut(s) 77, 530, 740
Hpy8I GTNNAC 3 cut(s) 271, 379, 381
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 4 cut(s) 585, 604, 847, 967
HpyCH4III ACNGT 5 cut(s) 106, 364, 568, 634, 1084
HpyCH4V TGCA 4 cut(s) 224, 356, 746, 966
HpyF10VI GCNNNNNNNGC 2 cut(s) 79, 1141
HpyF3I CTNAG 5 cut(s) 197, 838, 870, 901, 1130
Hsp92II CATG 8 cut(s) 86, 257, 433, 698, 743, 998, 1122, 1148
HspAI GCGC 1 cut(s) 716
Kzo9I GATC 4 cut(s) 110, 311, 1054, 1172
LmnI GCTCC 3 cut(s) 161, 180, 323
Lsp1109I GCAGC 3 cut(s) 236, 950, 1122
LweI GCATC 2 cut(s) 227, 733
MaeI CTAG 2 cut(s) 387, 1185
MaeIII GTNAC 9 cut(s) 28, 86, 301, 449, 562, 650, 1037, 1090, 1148
MalI GATC 4 cut(s) 112, 313, 1056, 1174
MboI GATC 4 cut(s) 110, 311, 1054, 1172
MboII GAAGA 7 cut(s) 179, 437, 517, 639, 800, 981, 1079
MfeI CAATTG 2 cut(s) 760, 783
MflI RGATCY 1 cut(s) 1172
MhlI GDGCHC 1 cut(s) 328
MlsI TGGCCA 1 cut(s) 797
MluCI AATT 9 cut(s) 533, 570, 679, 760, 783, 815, 824, 917, 954
MluNI TGGCCA 1 cut(s) 797
MlyI GAGTC 3 cut(s) 854, 971, 1011
MmeI TCCRAC 1 cut(s) 223
Mox20I TGGCCA 1 cut(s) 797
MscI TGGCCA 1 cut(s) 797
MseI TTAA 6 cut(s) 137, 408, 465, 473, 861, 921
MslI CAYNNNNRTG 2 cut(s) 699, 741
Msp20I TGGCCA 1 cut(s) 797
MspI CCGG 2 cut(s) 209, 266
MspR9I CCNGG 5 cut(s) 58, 159, 209, 615, 879
MunI CAATTG 2 cut(s) 760, 783
MvaI CCWGG 4 cut(s) 58, 159, 615, 879
MwoI GCNNNNNNNGC 2 cut(s) 79, 1141
NciI CCSGG 1 cut(s) 209
NcoI CCATGG 1 cut(s) 994
NdeII GATC 4 cut(s) 110, 311, 1054, 1172
NlaIII CATG 8 cut(s) 86, 257, 433, 698, 743, 998, 1122, 1148
NlaIV GGNNCC 5 cut(s) 55, 176, 288, 325, 592
NmuCI GTSAC 4 cut(s) 301, 449, 562, 1037
NspI RCATGY 1 cut(s) 698
NspV TTCGAA 3 cut(s) 503, 625, 732
PagI TCATGA 1 cut(s) 739
PciI ACATGT 1 cut(s) 694
PfeI GAWTC 3 cut(s) 874, 983, 1126
PkrI GCNGC 3 cut(s) 226, 965, 1137
PleI GAGTC 3 cut(s) 853, 971, 1010
PpsI GAGTC 3 cut(s) 853, 971, 1010
PpuMI RGGWCCY 2 cut(s) 54, 866
PscI ACATGT 1 cut(s) 694
Psp5II RGGWCCY 2 cut(s) 54, 866
Psp6I CCWGG 4 cut(s) 56, 157, 613, 877
PspGI CCWGG 4 cut(s) 56, 157, 613, 877
PspN4I GGNNCC 5 cut(s) 55, 176, 288, 325, 592
PspPI GGNCC 3 cut(s) 54, 590, 866
PspPPI RGGWCCY 2 cut(s) 54, 866
PsuI RGATCY 1 cut(s) 1172
RsaI GTAC 3 cut(s) 380, 693, 1013
RsaNI GTAC 3 cut(s) 379, 692, 1012
RseI CAYNNNNRTG 2 cut(s) 699, 741
SaqAI TTAA 6 cut(s) 137, 408, 465, 473, 861, 921
SatI GCNGC 3 cut(s) 225, 964, 1136
Sau3AI GATC 4 cut(s) 110, 311, 1054, 1172
Sau96I GGNCC 3 cut(s) 54, 590, 866
SchI GAGTC 3 cut(s) 854, 971, 1011
ScrFI CCNGG 5 cut(s) 58, 159, 209, 615, 879
SduI GDGCHC 1 cut(s) 328
SfaNI GCATC 2 cut(s) 227, 733
SfuI TTCGAA 3 cut(s) 503, 625, 732
SinI GGWCC 2 cut(s) 54, 866
SmiMI CAYNNNNRTG 2 cut(s) 699, 741
SmlI CTYRAG 2 cut(s) 77, 338
SmoI CTYRAG 2 cut(s) 77, 338
Sse9I AATT 9 cut(s) 533, 570, 679, 760, 783, 815, 824, 917, 954
SsiI CCGC 1 cut(s) 999
SspI AATATT 1 cut(s) 183
SspMI CTAG 2 cut(s) 387, 1185
StyD4I CCNGG 5 cut(s) 56, 157, 207, 613, 877
StyI CCWWGG 3 cut(s) 232, 328, 994
TaaI ACNGT 5 cut(s) 106, 364, 568, 634, 1084
TaqI TCGA 8 cut(s) 318, 497, 503, 625, 732, 770, 1061, 1159
TaqII GACCGA 1 cut(s) 1047
TasI AATT 9 cut(s) 533, 570, 679, 760, 783, 815, 824, 917, 954
TatI WGTACW 2 cut(s) 378, 1011
TfiI GAWTC 3 cut(s) 874, 983, 1126
Tru1I TTAA 6 cut(s) 137, 408, 465, 473, 861, 921
Tru9I TTAA 6 cut(s) 137, 408, 465, 473, 861, 921
TscAI CASTG 5 cut(s) 109, 369, 639, 693, 965
TseFI GTSAC 4 cut(s) 301, 449, 562, 1037
TseI GCWGC 3 cut(s) 224, 963, 1135
Tsp45I GTSAC 4 cut(s) 301, 449, 562, 1037
TspDTI ATGAA 1 cut(s) 37
TspGWI ACGGA 1 cut(s) 1013
TspRI CASTG 5 cut(s) 109, 369, 639, 693, 965
VpaK11BI GGWCC 2 cut(s) 54, 866
XapI RAATTY 4 cut(s) 533, 815, 824, 917
XceI RCATGY 1 cut(s) 698
XspI CTAG 2 cut(s) 387, 1185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.