Encoded by
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 2 cut(s) | 152, 338 |
| AcuI | CTGAAG | 1 cut(s) | 402 |
| AfaI | GTAC | 1 cut(s) | 357 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 48, 393 |
| AflII | CTTAAG | 1 cut(s) | 283 |
| AflIII | ACRYGT | 1 cut(s) | 23 |
| AgsI | TTSAA | 4 cut(s) | 151, 170, 212, 296 |
| AluBI | AGCT | 3 cut(s) | 146, 166, 182 |
| AluI | AGCT | 3 cut(s) | 146, 166, 182 |
| Alw26I | GTCTC | 2 cut(s) | 32, 244 |
| AlwI | GGATC | 2 cut(s) | 152, 338 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 98 |
| AsuHPI | GGTGA | 1 cut(s) | 92 |
| BauI | CACGAG | 1 cut(s) | 391 |
| BbsI | GAAGAC | 1 cut(s) | 55 |
| BcoDI | GTCTC | 2 cut(s) | 32, 244 |
| BfaI | CTAG | 1 cut(s) | 272 |
| BfrI | CTTAAG | 1 cut(s) | 283 |
| BmsI | GCATC | 1 cut(s) | 312 |
| BpiI | GAAGAC | 1 cut(s) | 55 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 211, 243 |
| Bpu10I | CCTNAGC | 1 cut(s) | 162 |
| BsaBI | GATNNNNATC | 1 cut(s) | 342 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 48, 393 |
| Bse8I | GATNNNNATC | 1 cut(s) | 342 |
| BseGI | GGATG | 1 cut(s) | 160 |
| BseJI | GATNNNNATC | 1 cut(s) | 342 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 48, 393 |
| BseMII | CTCAG | 2 cut(s) | 59, 153 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 48, 393 |
| BsmAI | GTCTC | 2 cut(s) | 32, 244 |
| BsmI | GAATGC | 1 cut(s) | 302 |
| Bsp1407I | TGTACA | 1 cut(s) | 355 |
| Bsp143I | GATC | 2 cut(s) | 157, 343 |
| BspCNI | CTCAG | 2 cut(s) | 60, 154 |
| BspPI | GGATC | 2 cut(s) | 152, 338 |
| BspTI | CTTAAG | 1 cut(s) | 283 |
| BsrGI | TGTACA | 1 cut(s) | 355 |
| BssMI | GATC | 2 cut(s) | 157, 343 |
| BssSI | CACGAG | 1 cut(s) | 391 |
| Bst2BI | CACGAG | 1 cut(s) | 391 |
| Bst4CI | ACNGT | 1 cut(s) | 319 |
| Bst6I | CTCTTC | 1 cut(s) | 304 |
| BstAFI | CTTAAG | 1 cut(s) | 283 |
| BstAUI | TGTACA | 1 cut(s) | 355 |
| BstDEI | CTNAG | 2 cut(s) | 68, 162 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 391 |
| BstF5I | GGATG | 1 cut(s) | 160 |
| BstKTI | GATC | 2 cut(s) | 160, 346 |
| BstMAI | GTCTC | 2 cut(s) | 32, 244 |
| BstMBI | GATC | 2 cut(s) | 157, 343 |
| BstNSI | RCATGY | 1 cut(s) | 27 |
| BstV2I | GAAGAC | 1 cut(s) | 55 |
| BtsCI | GGATG | 1 cut(s) | 160 |
| BtsIMutI | CAGTG | 1 cut(s) | 324 |
| Csp6I | GTAC | 1 cut(s) | 356 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 180, 215 |
| CviAII | CATG | 4 cut(s) | 24, 264, 301, 398 |
| CviJI | RGCY | 5 cut(s) | 99, 146, 166, 182, 386 |
| CviKI_1 | RGCY | 5 cut(s) | 99, 146, 166, 182, 386 |
| CviQI | GTAC | 1 cut(s) | 356 |
| DdeI | CTNAG | 2 cut(s) | 68, 162 |
| DpnI | GATC | 2 cut(s) | 159, 345 |
| DpnII | GATC | 2 cut(s) | 157, 343 |
| Eam1104I | CTCTTC | 1 cut(s) | 304 |
| EarI | CTCTTC | 1 cut(s) | 304 |
| Eco57I | CTGAAG | 1 cut(s) | 402 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 391 |
| EcoT22I | ATGCAT | 1 cut(s) | 302 |
| FaeI | CATG | 4 cut(s) | 27, 267, 304, 401 |
| FatI | CATG | 4 cut(s) | 23, 263, 300, 397 |
| FokI | GGATG | 1 cut(s) | 167 |
| FspBI | CTAG | 1 cut(s) | 272 |
| Hin1II | CATG | 4 cut(s) | 27, 267, 304, 401 |
| HincII | GTYRAC | 1 cut(s) | 261 |
| HindII | GTYRAC | 1 cut(s) | 261 |
| HinfI | GANTC | 1 cut(s) | 289 |
| HphI | GGTGA | 1 cut(s) | 92 |
| Hpy166II | GTNNAC | 1 cut(s) | 261 |
| Hpy188III | TCNNGA | 6 cut(s) | 67, 161, 243, 272, 293, 391 |
| Hpy8I | GTNNAC | 1 cut(s) | 261 |
| HpyAV | CCTTC | 3 cut(s) | 88, 299, 397 |
| HpyCH4III | ACNGT | 1 cut(s) | 319 |
| HpyCH4V | TGCA | 4 cut(s) | 38, 122, 206, 300 |
| HpyF3I | CTNAG | 2 cut(s) | 68, 162 |
| Hsp92II | CATG | 4 cut(s) | 27, 267, 304, 401 |
| Kzo9I | GATC | 2 cut(s) | 157, 343 |
| LpnPI | CCDG | 5 cut(s) | 48, 80, 174, 354, 361 |
| LweI | GCATC | 1 cut(s) | 312 |
| MaeI | CTAG | 1 cut(s) | 272 |
| MaeIII | GTNAC | 1 cut(s) | 80 |
| MalI | GATC | 2 cut(s) | 159, 345 |
| MboI | GATC | 2 cut(s) | 157, 343 |
| MboII | GAAGA | 4 cut(s) | 55, 224, 321, 413 |
| MfeI | CAATTG | 1 cut(s) | 207 |
| MluCI | AATT | 4 cut(s) | 207, 215, 247, 327 |
| MnlI | CCTC | 5 cut(s) | 28, 42, 54, 71, 387 |
| Mph1103I | ATGCAT | 1 cut(s) | 302 |
| MroXI | GAANNNNTTC | 1 cut(s) | 98 |
| MseI | TTAA | 1 cut(s) | 284 |
| MspCI | CTTAAG | 1 cut(s) | 283 |
| MunI | CAATTG | 1 cut(s) | 207 |
| Mva1269I | GAATGC | 1 cut(s) | 302 |
| NdeII | GATC | 2 cut(s) | 157, 343 |
| NlaIII | CATG | 4 cut(s) | 27, 267, 304, 401 |
| NmuCI | GTSAC | 1 cut(s) | 80 |
| NsiI | ATGCAT | 1 cut(s) | 302 |
| NspI | RCATGY | 1 cut(s) | 27 |
| PciI | ACATGT | 1 cut(s) | 23 |
| PctI | GAATGC | 1 cut(s) | 302 |
| PdmI | GAANNNNTTC | 1 cut(s) | 98 |
| PfeI | GAWTC | 1 cut(s) | 289 |
| PscI | ACATGT | 1 cut(s) | 23 |
| RsaI | GTAC | 1 cut(s) | 357 |
| RsaNI | GTAC | 1 cut(s) | 356 |
| SaqAI | TTAA | 1 cut(s) | 284 |
| Sau3AI | GATC | 2 cut(s) | 157, 343 |
| SetI | ASST | 6 cut(s) | 20, 82, 148, 168, 184, 411 |
| SfaNI | GCATC | 1 cut(s) | 312 |
| SmlI | CTYRAG | 1 cut(s) | 283 |
| SmoI | CTYRAG | 1 cut(s) | 283 |
| Sse9I | AATT | 4 cut(s) | 207, 215, 247, 327 |
| SspMI | CTAG | 1 cut(s) | 272 |
| TaaI | ACNGT | 1 cut(s) | 319 |
| TaqI | TCGA | 1 cut(s) | 342 |
| TasI | AATT | 4 cut(s) | 207, 215, 247, 327 |
| TatI | WGTACW | 1 cut(s) | 355 |
| TfiI | GAWTC | 1 cut(s) | 289 |
| Tru1I | TTAA | 1 cut(s) | 284 |
| Tru9I | TTAA | 1 cut(s) | 284 |
| TscAI | CASTG | 1 cut(s) | 324 |
| TseFI | GTSAC | 1 cut(s) | 80 |
| Tsp45I | GTSAC | 1 cut(s) | 80 |
| TspDTI | ATGAA | 2 cut(s) | 291, 414 |
| TspRI | CASTG | 1 cut(s) | 324 |
| Vha464I | CTTAAG | 1 cut(s) | 283 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 391 |
| XbaI | TCTAGA | 1 cut(s) | 271 |
| XceI | RCATGY | 1 cut(s) | 27 |
| XmnI | GAANNNNTTC | 1 cut(s) | 98 |
| XspI | CTAG | 1 cut(s) | 272 |
| Zsp2I | ATGCAT | 1 cut(s) | 302 |