Male sterility protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 2 cut(s) | 105, 564 |
| AcoI | YGGCCR | 1 cut(s) | 393 |
| AcsI | RAATTY | 1 cut(s) | 533 |
| AcuI | CTGAAG | 2 cut(s) | 62, 144 |
| AcyI | GRCGYC | 1 cut(s) | 225 |
| AfaI | GTAC | 3 cut(s) | 407, 626, 758 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 125, 647 |
| AgeI | ACCGGT | 1 cut(s) | 242 |
| AgsI | TTSAA | 5 cut(s) | 220, 278, 340, 425, 734 |
| AjnI | CCWGG | 3 cut(s) | 160, 315, 528 |
| AluBI | AGCT | 3 cut(s) | 144, 478, 486 |
| AluI | AGCT | 3 cut(s) | 144, 478, 486 |
| Alw21I | GWGCWC | 3 cut(s) | 169, 488, 696 |
| Alw26I | GTCTC | 1 cut(s) | 361 |
| Alw44I | GTGCAC | 1 cut(s) | 692 |
| AlwI | GGATC | 2 cut(s) | 105, 564 |
| Ama87I | CYCGRG | 1 cut(s) | 290 |
| AoxI | GGCC | 1 cut(s) | 393 |
| ApaLI | GTGCAC | 1 cut(s) | 692 |
| ApeKI | GCWGC | 2 cut(s) | 73, 237 |
| ApoI | RAATTY | 1 cut(s) | 533 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 582, 614 |
| AsiGI | ACCGGT | 1 cut(s) | 242 |
| AspS9I | GGNCC | 1 cut(s) | 590 |
| AsuHPI | GGTGA | 2 cut(s) | 254, 662 |
| AvaI | CYCGRG | 1 cut(s) | 290 |
| AvaII | GGWCC | 1 cut(s) | 590 |
| BaeGI | GKGCMC | 2 cut(s) | 696, 718 |
| BalI | TGGCCA | 1 cut(s) | 395 |
| BanII | GRGCYC | 2 cut(s) | 316, 488 |
| BbsI | GAAGAC | 1 cut(s) | 241 |
| Bbv12I | GWGCWC | 3 cut(s) | 169, 488, 696 |
| BbvI | GCAGC | 2 cut(s) | 60, 224 |
| BccI | CCATC | 1 cut(s) | 236 |
| BciT130I | CCWGG | 3 cut(s) | 162, 317, 530 |
| BcoDI | GTCTC | 1 cut(s) | 361 |
| BfaI | CTAG | 3 cut(s) | 329, 765, 772 |
| BfmI | CTRYAG | 1 cut(s) | 579 |
| BglII | AGATCT | 1 cut(s) | 196 |
| BisI | GCNGC | 2 cut(s) | 74, 238 |
| BlsI | GCNGC | 2 cut(s) | 75, 239 |
| Bme1390I | CCNGG | 3 cut(s) | 162, 317, 530 |
| Bme18I | GGWCC | 1 cut(s) | 590 |
| BmeT110I | CYCGRG | 1 cut(s) | 290 |
| BmgT120I | GGNCC | 1 cut(s) | 590 |
| BmiI | GGNNCC | 2 cut(s) | 592, 639 |
| BmrFI | CCNGG | 3 cut(s) | 162, 317, 530 |
| BpiI | GAAGAC | 1 cut(s) | 241 |
| BsaHI | GRCGYC | 1 cut(s) | 225 |
| BsaJI | CCNNGG | 5 cut(s) | 232, 267, 291, 315, 528 |
| BsaWI | WCCGGW | 1 cut(s) | 242 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 125, 647 |
| Bse118I | RCCGGY | 1 cut(s) | 242 |
| Bse1I | ACTGG | 2 cut(s) | 37, 387 |
| Bse3DI | GCAATG | 1 cut(s) | 668 |
| BseBI | CCWGG | 3 cut(s) | 162, 317, 530 |
| BseDI | CCNNGG | 5 cut(s) | 232, 267, 291, 315, 528 |
| BseGI | GGATG | 2 cut(s) | 228, 383 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 125, 647 |
| BseMI | GCAATG | 1 cut(s) | 668 |
| BseMII | CTCAG | 1 cut(s) | 59 |
| BseNI | ACTGG | 2 cut(s) | 37, 387 |
| BseSI | GKGCMC | 2 cut(s) | 696, 718 |
| BseXI | GCAGC | 2 cut(s) | 60, 224 |
| BsgI | GTGCAG | 1 cut(s) | 223 |
| BshFI | GGCC | 1 cut(s) | 395 |
| BshTI | ACCGGT | 1 cut(s) | 242 |
| BsiHKAI | GWGCWC | 3 cut(s) | 169, 488, 696 |
| BsiHKCI | CYCGRG | 1 cut(s) | 290 |
| BsiSI | CCGG | 1 cut(s) | 243 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 125, 647 |
| BsmAI | GTCTC | 1 cut(s) | 361 |
| BsmBI | CGTCTC | 1 cut(s) | 361 |
| BsnI | GGCC | 1 cut(s) | 395 |
| BsoBI | CYCGRG | 1 cut(s) | 290 |
| Bsp1286I | GDGCHC | 5 cut(s) | 169, 316, 488, 696, 718 |
| Bsp1407I | TGTACA | 2 cut(s) | 405, 624 |
| Bsp143I | GATC | 3 cut(s) | 110, 196, 556 |
| Bsp19I | CCATGG | 1 cut(s) | 267 |
| BspANI | GGCC | 1 cut(s) | 395 |
| BspCNI | CTCAG | 1 cut(s) | 60 |
| BspLI | GGNNCC | 2 cut(s) | 592, 639 |
| BspPI | GGATC | 2 cut(s) | 105, 564 |
| BspQI | GCTCTTC | 1 cut(s) | 493 |
| BsrDI | GCAATG | 1 cut(s) | 668 |
| BsrFI | RCCGGY | 1 cut(s) | 242 |
| BsrGI | TGTACA | 2 cut(s) | 405, 624 |
| BsrI | ACTGG | 2 cut(s) | 37, 387 |
| BssAI | RCCGGY | 1 cut(s) | 242 |
| BssECI | CCNNGG | 5 cut(s) | 232, 267, 291, 315, 528 |
| BssMI | GATC | 3 cut(s) | 110, 196, 556 |
| BssNI | GRCGYC | 1 cut(s) | 225 |
| BssT1I | CCWWGG | 2 cut(s) | 232, 267 |
| Bst2UI | CCWGG | 3 cut(s) | 162, 317, 530 |
| Bst4CI | ACNGT | 4 cut(s) | 22, 301, 364, 568 |
| Bst6I | CTCTTC | 1 cut(s) | 493 |
| BstACI | GRCGYC | 1 cut(s) | 225 |
| BstAUI | TGTACA | 2 cut(s) | 405, 624 |
| BstC8I | GCNNGC | 2 cut(s) | 346, 416 |
| BstDEI | CTNAG | 1 cut(s) | 68 |
| BstDSI | CCRYGG | 1 cut(s) | 267 |
| BstEII | GGTNACC | 1 cut(s) | 650 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 123 |
| BstF5I | GGATG | 2 cut(s) | 228, 383 |
| BstKTI | GATC | 3 cut(s) | 113, 199, 559 |
| BstMAI | GTCTC | 1 cut(s) | 361 |
| BstMBI | GATC | 3 cut(s) | 110, 196, 556 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 79, 354 |
| BstNI | CCWGG | 3 cut(s) | 162, 317, 530 |
| BstNSI | RCATGY | 3 cut(s) | 348, 740, 763 |
| BstPI | GGTNACC | 1 cut(s) | 650 |
| BstSCI | CCNGG | 3 cut(s) | 160, 315, 528 |
| BstSFI | CTRYAG | 1 cut(s) | 579 |
| BstSLI | GKGCMC | 2 cut(s) | 696, 718 |
| BstV1I | GCAGC | 2 cut(s) | 60, 224 |
| BstV2I | GAAGAC | 1 cut(s) | 241 |
| BstX2I | RGATCY | 3 cut(s) | 110, 196, 556 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 511 |
| BstYI | RGATCY | 3 cut(s) | 110, 196, 556 |
| BsuRI | GGCC | 1 cut(s) | 395 |
| BtgI | CCRYGG | 1 cut(s) | 267 |
| BtsCI | GGATG | 2 cut(s) | 228, 383 |
| BtsI | GCAGTG | 1 cut(s) | 546 |
| BtsIMutI | CAGTG | 2 cut(s) | 546, 564 |
| Cac8I | GCNNGC | 2 cut(s) | 346, 416 |
| Cfr10I | RCCGGY | 1 cut(s) | 242 |
| Cfr13I | GGNCC | 1 cut(s) | 590 |
| CseI | GACGC | 2 cut(s) | 233, 407 |
| Csp6I | GTAC | 3 cut(s) | 406, 625, 757 |
| CspAI | ACCGGT | 1 cut(s) | 242 |
| CviQI | GTAC | 3 cut(s) | 406, 625, 757 |
| DdeI | CTNAG | 1 cut(s) | 68 |
| DpnI | GATC | 3 cut(s) | 112, 198, 558 |
| DpnII | GATC | 3 cut(s) | 110, 196, 556 |
| EaeI | YGGCCR | 1 cut(s) | 393 |
| Eam1104I | CTCTTC | 1 cut(s) | 493 |
| EarI | CTCTTC | 1 cut(s) | 493 |
| Ecl136II | GAGCTC | 1 cut(s) | 486 |
| Eco130I | CCWWGG | 2 cut(s) | 232, 267 |
| Eco24I | GRGCYC | 2 cut(s) | 316, 488 |
| Eco32I | GATATC | 1 cut(s) | 139 |
| Eco47I | GGWCC | 1 cut(s) | 590 |
| Eco53kI | GAGCTC | 1 cut(s) | 486 |
| Eco57I | CTGAAG | 2 cut(s) | 62, 144 |
| Eco88I | CYCGRG | 1 cut(s) | 290 |
| Eco91I | GGTNACC | 1 cut(s) | 650 |
| EcoICRI | GAGCTC | 1 cut(s) | 486 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 123 |
| EcoO65I | GGTNACC | 1 cut(s) | 650 |
| EcoRII | CCWGG | 3 cut(s) | 160, 315, 528 |
| EcoRV | GATATC | 1 cut(s) | 139 |
| EcoT14I | CCWWGG | 2 cut(s) | 232, 267 |
| EcoT38I | GRGCYC | 2 cut(s) | 316, 488 |
| ErhI | CCWWGG | 2 cut(s) | 232, 267 |
| Esp3I | CGTCTC | 1 cut(s) | 361 |
| Fnu4HI | GCNGC | 2 cut(s) | 74, 238 |
| FokI | GGATG | 2 cut(s) | 215, 370 |
| FriOI | GRGCYC | 2 cut(s) | 316, 488 |
| Fsp4HI | GCNGC | 2 cut(s) | 74, 238 |
| FspBI | CTAG | 3 cut(s) | 329, 765, 772 |
| GluI | GCNGC | 2 cut(s) | 74, 238 |
| HaeIII | GGCC | 1 cut(s) | 395 |
| HapII | CCGG | 1 cut(s) | 243 |
| HgaI | GACGC | 2 cut(s) | 233, 407 |
| Hin1I | GRCGYC | 1 cut(s) | 225 |
| HindIII | AAGCTT | 1 cut(s) | 476 |
| HinfI | GANTC | 2 cut(s) | 64, 508 |
| HpaII | CCGG | 1 cut(s) | 243 |
| HphI | GGTGA | 2 cut(s) | 254, 662 |
| Hpy166II | GTNNAC | 3 cut(s) | 379, 625, 694 |
| Hpy188I | TCNGA | 2 cut(s) | 14, 69 |
| Hpy188III | TCNNGA | 2 cut(s) | 422, 731 |
| Hpy8I | GTNNAC | 3 cut(s) | 379, 625, 694 |
| Hpy99I | CGWCG | 1 cut(s) | 227 |
| HpyAV | CCTTC | 8 cut(s) | 119, 167, 226, 272, 314, 334, 604, 737 |
| HpyCH4III | ACNGT | 4 cut(s) | 22, 301, 364, 568 |
| HpyCH4IV | ACGT | 1 cut(s) | 273 |
| HpyCH4V | TGCA | 7 cut(s) | 194, 240, 357, 465, 539, 694, 746 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 79, 354 |
| HpyF3I | CTNAG | 1 cut(s) | 68 |
| HpySE526I | ACGT | 1 cut(s) | 273 |
| Hsp92I | GRCGYC | 1 cut(s) | 225 |
| Kzo9I | GATC | 3 cut(s) | 110, 196, 556 |
| LguI | GCTCTTC | 1 cut(s) | 493 |
| LmnI | GCTCC | 2 cut(s) | 164, 643 |
| Lsp1109I | GCAGC | 2 cut(s) | 60, 224 |
| MaeI | CTAG | 3 cut(s) | 329, 765, 772 |
| MaeII | ACGT | 1 cut(s) | 273 |
| MaeIII | GTNAC | 6 cut(s) | 28, 86, 301, 470, 562, 650 |
| MalI | GATC | 3 cut(s) | 112, 198, 558 |
| MboI | GATC | 3 cut(s) | 110, 196, 556 |
| MboII | GAAGA | 6 cut(s) | 17, 178, 241, 437, 480, 715 |
| MflI | RGATCY | 3 cut(s) | 110, 196, 556 |
| MhlI | GDGCHC | 5 cut(s) | 169, 316, 488, 696, 718 |
| MlsI | TGGCCA | 1 cut(s) | 395 |
| MluCI | AATT | 1 cut(s) | 533 |
| MluNI | TGGCCA | 1 cut(s) | 395 |
| MlyI | GAGTC | 1 cut(s) | 502 |
| MmeI | TCCRAC | 2 cut(s) | 138, 568 |
| MnlI | CCTC | 8 cut(s) | 8, 63, 64, 286, 445, 513, 537, 747 |
| Mox20I | TGGCCA | 1 cut(s) | 395 |
| MscI | TGGCCA | 1 cut(s) | 395 |
| MslI | CAYNNNNRTG | 5 cut(s) | 349, 632, 686, 699, 741 |
| Msp20I | TGGCCA | 1 cut(s) | 395 |
| MspI | CCGG | 1 cut(s) | 243 |
| MspR9I | CCNGG | 3 cut(s) | 162, 317, 530 |
| MvaI | CCWGG | 3 cut(s) | 162, 317, 530 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 79, 354 |
| NcoI | CCATGG | 1 cut(s) | 267 |
| NdeII | GATC | 3 cut(s) | 110, 196, 556 |
| NlaIV | GGNNCC | 2 cut(s) | 592, 639 |
| NmuCI | GTSAC | 2 cut(s) | 562, 650 |
| NspI | RCATGY | 3 cut(s) | 348, 740, 763 |
| PaeI | GCATGC | 1 cut(s) | 348 |
| PciSI | GCTCTTC | 1 cut(s) | 493 |
| PfeI | GAWTC | 1 cut(s) | 64 |
| PfoI | TCCNGGA | 1 cut(s) | 160 |
| PinAI | ACCGGT | 1 cut(s) | 242 |
| PkrI | GCNGC | 2 cut(s) | 75, 239 |
| PleI | GAGTC | 1 cut(s) | 502 |
| PpsI | GAGTC | 1 cut(s) | 502 |
| Psp124BI | GAGCTC | 1 cut(s) | 488 |
| Psp6I | CCWGG | 3 cut(s) | 160, 315, 528 |
| PspEI | GGTNACC | 1 cut(s) | 650 |
| PspGI | CCWGG | 3 cut(s) | 160, 315, 528 |
| PspN4I | GGNNCC | 2 cut(s) | 592, 639 |
| PspPI | GGNCC | 1 cut(s) | 590 |
| PsuI | RGATCY | 3 cut(s) | 110, 196, 556 |
| RsaI | GTAC | 3 cut(s) | 407, 626, 758 |
| RsaNI | GTAC | 3 cut(s) | 406, 625, 757 |
| RseI | CAYNNNNRTG | 5 cut(s) | 349, 632, 686, 699, 741 |
| SacI | GAGCTC | 1 cut(s) | 488 |
| SapI | GCTCTTC | 1 cut(s) | 493 |
| SatI | GCNGC | 2 cut(s) | 74, 238 |
| Sau3AI | GATC | 3 cut(s) | 110, 196, 556 |
| Sau96I | GGNCC | 1 cut(s) | 590 |
| SchI | GAGTC | 1 cut(s) | 502 |
| ScrFI | CCNGG | 3 cut(s) | 162, 317, 530 |
| SduI | GDGCHC | 5 cut(s) | 169, 316, 488, 696, 718 |
| SfcI | CTRYAG | 1 cut(s) | 579 |
| SgrAI | CRCCGGYG | 1 cut(s) | 242 |
| SinI | GGWCC | 1 cut(s) | 590 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 349, 632, 686, 699, 741 |
| SphI | GCATGC | 1 cut(s) | 348 |
| Sse9I | AATT | 1 cut(s) | 533 |
| SspI | AATATT | 1 cut(s) | 402 |
| SspMI | CTAG | 3 cut(s) | 329, 765, 772 |
| SstI | GAGCTC | 1 cut(s) | 488 |
| StyD4I | CCNGG | 3 cut(s) | 160, 315, 528 |
| StyI | CCWWGG | 2 cut(s) | 232, 267 |
| TaaI | ACNGT | 4 cut(s) | 22, 301, 364, 568 |
| TaiI | ACGT | 1 cut(s) | 276 |
| TaqI | TCGA | 1 cut(s) | 97 |
| TasI | AATT | 1 cut(s) | 533 |
| TatI | WGTACW | 2 cut(s) | 405, 624 |
| TfiI | GAWTC | 1 cut(s) | 64 |
| TscAI | CASTG | 2 cut(s) | 546, 571 |
| TseFI | GTSAC | 2 cut(s) | 562, 650 |
| TseI | GCWGC | 2 cut(s) | 73, 237 |
| Tsp45I | GTSAC | 2 cut(s) | 562, 650 |
| TspDTI | ATGAA | 1 cut(s) | 450 |
| TspGWI | ACGGA | 1 cut(s) | 610 |
| TspRI | CASTG | 2 cut(s) | 546, 571 |
| VneI | GTGCAC | 1 cut(s) | 692 |
| VpaK11BI | GGWCC | 1 cut(s) | 590 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 123 |
| XapI | RAATTY | 1 cut(s) | 533 |
| XceI | RCATGY | 3 cut(s) | 348, 740, 763 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 313, 511 |
| XspI | CTAG | 3 cut(s) | 329, 765, 772 |