Rh1DG221900

3-beta hydroxysteroid dehydrogenase/isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000382
Physical Location & Seq
Forward (+)
43170691 .. 43172721
2031 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG221900.1

Sequence Viewer

Length: 1005 bp
ATGGAAGAGGAAAAGGGTCCAGTTTGTGTAACGGGTGGAACTGGATTCATCGGTTCATGGCTGGTTATGAAGCTTCTGCAAAATGGTTACACTGTTCGAACTACTGTTAGACCTGATCCAGAATGCAAGAGAGACATCAGCTACCTCACAAGCCTGCCTAGAGCATCGGAGAAGCTTCACATCTTCAATGGGGATCTGAACCAACCTGAAAGTTTTAATGTAGCAATTGAAGGATGCACCGGGGTCTTCCATGTTGCTCATCCTATGCCTAACAAAGAACTTGATGAAGCATCGGTAACCAAACAATCTGTAGAAGGTACCCTAGGCATATTGAAAGCTTGCCTAAATGCCAAGACTGTGAAAAAGGTTGTTTACACTTCTAGTGCAGCAACTGTTGCTTATAGTGGCAACAACAAAGACATGGCGGATGAGAGTTCATGGAGTGATATAGAGTACCATAGGTCTCTTGGATTATTTAGGAGTTCATCGTATGTGGCTGCTAAGACCAAAACAGAGCAAGCAGTACTGGAATTTGCAGAAAAAAATGGATTAGAACTTGTGACTTTGATCCCTCCATTCGTCCTTGGTGGTTTCATCTGCAAAGATCTCCCTGGTTCAGTGGAATTGATCTTATCCATGATATTAGGAAAAGCAGATAACTTTACACAACTTCACAAGCTAAGTTTGGTACATGTAGATGATTTGGTTAGTGCGCATATCTTTCTTTTTGAAAATTCTAATGCCAAAGGGAGGTACATTTGCTCTTCAACTCCAACATCAATAGACGAAATGTACCGATTTCTTTATGCAAAATACCCTGAATTTCGCATACCCACAACTCAATCACTGAAAGAAGCTTACAAAATTTGTGGTTTCTCATCGAAGAAGCTCCTGAATACTGGATTCACATTTAAGCATGGCCTCGATGACATATTGACTGGAGCATTTCAATCTTGCAGAGAAAAGGGTTTTCTAGAAAGGATCTTTGGCAAAATGGACACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

334

Amino Acids

36.96

Weight (kDa)

6.71

Isoelectric Point (pI)

39.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 135 1.4e-08 NmrA-like family
Epimerase PF01370 8 - 248 4.9e-24 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 8 - 132 7e-06 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 10 - 241 4e-18 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 10 - 207 9.8e-14 Male sterility protein
GDP_Man_Dehyd PF16363 10 - 191 1.3e-13 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 12 - 132 7.7e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45400 AT2G45400 AT2G45400 AT2G45400
fragaria_vesca FvH4_7g11750 FvH4_7g11760 FvH4_7g11780 FvH4_7g11781 FvH4_7g11781 FvH4_7g11790 FvH4_7g11790
malus_domestica MD02G1205000.v1.1 MD07G1121700.v1.1 MD08G1231300.v1.1 MD08G1231400.v1.1 MD08G1231500.v1.1
prunus_persica Prupe.2G144600_v2.0.a1 Prupe.2G144700_v2.0.a1 Prupe.2G144800_v2.0.a1
pyrus_communis pycom08g20120
rosa_chinensis RchiOBHm_Chr1g0350271 RchiOBHm_Chr1g0350281 RchiOBHm_Chr1g0350311 RchiOBHm_Chr1g0350321 RchiOBHm_Chr1g0350341 RchiOBHm_Chr1g0350361 RchiOBHm_Chr1g0350371 RchiOBHm_Chr1g0350401 RchiOBHm_Chr1g0350411 RchiOBHm_Chr1g0350431 RchiOBHm_Chr1g0350441 RchiOBHm_Chr1g0350471
rosa_laevigata RLG00000028483 RLG00000028485 RLG00000028486 RLG00000028487 RLG00000028488 RLG00000028489 RLG00000028493 RLG00000028494 RLG00000035411
rosa_multiflora Rmu_co8173820.1_g000001 Rmu_sc0001037.1_g000006 Rmu_sc0001864.1_g000002 Rmu_sc0001864.1_g000009 Rmu_ssc0000083.1_g000008 Rmu_ssc0000083.1_g000013 Rmu_ssc0000083.1_g000018
rosa_roxburghii Rroxscaffold_4G00304840 Rroxscaffold_4G00304860 Rroxscaffold_4G00304890 Rroxscaffold_4G00304900 Rroxscaffold_4G00304920 Rroxscaffold_4G00304930
rosa_rugosa Rorug01G0210800 Rorug01G0210900 Rorug01G0211000 Rorug01G0211100 Rorug01G0211200 Rorug01G0211300 Rorug01G0211300 Rorug01G0211400
rosa_samantha Rh1AG225500 Rh1AG225600 Rh1AG225700 Rh1AG225800 Rh1AG225900 Rh1AG226000 Rh1BG193400 Rh1BG193600 Rh1BG193700 Rh1BG193800 Rh1BG193900 Rh1BG194000 Rh1BG194200 Rh1CG210300 Rh1CG210500 Rh1CG210600 Rh1CG210700 Rh1CG210900 Rh1DG221700 Rh1DG221900 Rh1DG222100 Rh1DG222200 Rh1DG222300 Rh1DG222400 Rh1DG222500 Rh2BG259100 Rh3DG280100 Rh5AG528000
rosa_wichuraiana Rw0G018730 Rw0G018740 Rw0G018750 Rw0G018760 Rw0G018770 Rw0G018790 Rw1G019390 Rw1G019410 Rw1G019420 Rw1G019430 Rw1G019440 Rw1G019450 Rw1G019480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 714
Acc65I GGTACC 1 cut(s) 317
AccB1I GGYRCC 1 cut(s) 317
AciI CCGC 1 cut(s) 425
AclWI GGATC 4 cut(s) 110, 201, 562, 989
AcsI RAATTY 4 cut(s) 530, 733, 821, 864
AfaI GTAC 6 cut(s) 319, 455, 525, 690, 755, 794
AfiI CCNNNNNNNGG 1 cut(s) 750
AflIII ACRYGT 1 cut(s) 691
AgsI TTSAA 6 cut(s) 187, 230, 334, 731, 768, 950
AjnI CCWGG 1 cut(s) 610
AjuI GAANNNNNNNTTGG 2 cut(s) 969, 1001
AluBI AGCT 7 cut(s) 73, 141, 175, 338, 679, 857, 889
AluI AGCT 7 cut(s) 73, 141, 175, 338, 679, 857, 889
Alw26I GTCTC 2 cut(s) 126, 468
AlwI GGATC 4 cut(s) 110, 201, 562, 989
AlwNI CAGNNNCTG 1 cut(s) 392
AoxI GGCC 1 cut(s) 919
ApeKI GCWGC 2 cut(s) 386, 497
ApoI RAATTY 4 cut(s) 530, 733, 821, 864
Asp718I GGTACC 1 cut(s) 317
AspA2I CCTAGG 1 cut(s) 322
AspLEI GCGC 1 cut(s) 715
AspS9I GGNCC 1 cut(s) 17
AsuC2I CCSGG 1 cut(s) 241
AsuII TTCGAA 1 cut(s) 97
AvaII GGWCC 1 cut(s) 17
AvrII CCTAGG 1 cut(s) 322
BanI GGYRCC 1 cut(s) 317
BbsI GAAGAC 1 cut(s) 238
BbvI GCAGC 2 cut(s) 398, 484
BciT130I CCWGG 1 cut(s) 612
BcnI CCSGG 1 cut(s) 241
BcoDI GTCTC 2 cut(s) 126, 468
BfaI CTAG 4 cut(s) 159, 323, 381, 974
BfmI CTRYAG 1 cut(s) 309
BglII AGATCT 1 cut(s) 604
BisI GCNGC 2 cut(s) 387, 498
BlnI CCTAGG 1 cut(s) 322
BlsI GCNGC 2 cut(s) 388, 499
BmcAI AGTACT 1 cut(s) 525
Bme1390I CCNGG 2 cut(s) 241, 612
Bme18I GGWCC 1 cut(s) 17
BmgT120I GGNCC 1 cut(s) 17
BmiI GGNNCC 2 cut(s) 18, 319
BmrFI CCNGG 2 cut(s) 241, 612
BmsI GCATC 3 cut(s) 173, 224, 299
BpiI GAAGAC 1 cut(s) 238
BpmI CTGGAG 1 cut(s) 960
Bpu14I TTCGAA 1 cut(s) 97
BpuMI CCSGG 1 cut(s) 241
BsaI GGTCTC 1 cut(s) 468
BsaJI CCNNGG 4 cut(s) 240, 322, 583, 610
Bsc4I CCNNNNNNNGG 1 cut(s) 750
Bse1I ACTGG 5 cut(s) 20, 46, 531, 904, 943
BseBI CCWGG 1 cut(s) 612
BseDI CCNNGG 4 cut(s) 240, 322, 583, 610
BseGI GGATG 3 cut(s) 239, 259, 433
BseLI CCNNNNNNNGG 1 cut(s) 750
BseNI ACTGG 5 cut(s) 20, 46, 531, 904, 943
BseXI GCAGC 2 cut(s) 398, 484
BsgI GTGCAG 1 cut(s) 405
BshFI GGCC 1 cut(s) 921
BshNI GGYRCC 1 cut(s) 317
BsiSI CCGG 1 cut(s) 240
BslI CCNNNNNNNGG 1 cut(s) 750
BsmAI GTCTC 2 cut(s) 126, 468
BsmI GAATGC 1 cut(s) 128
BsnI GGCC 1 cut(s) 921
Bso31I GGTCTC 1 cut(s) 468
Bsp119I TTCGAA 1 cut(s) 97
Bsp143I GATC 6 cut(s) 115, 193, 567, 604, 627, 981
BspACI CCGC 1 cut(s) 425
BspANI GGCC 1 cut(s) 921
BspLI GGNNCC 2 cut(s) 18, 319
BspPI GGATC 4 cut(s) 110, 201, 562, 989
BspQI GCTCTTC 1 cut(s) 769
BspT104I TTCGAA 1 cut(s) 97
BspT107I GGYRCC 1 cut(s) 317
BspTNI GGTCTC 1 cut(s) 468
BsrI ACTGG 5 cut(s) 20, 46, 531, 904, 943
BssECI CCNNGG 4 cut(s) 240, 322, 583, 610
BssMI GATC 6 cut(s) 115, 193, 567, 604, 627, 981
BssT1I CCWWGG 2 cut(s) 322, 583
Bst2UI CCWGG 1 cut(s) 612
Bst4CI ACNGT 4 cut(s) 94, 106, 358, 394
Bst6I CTCTTC 1 cut(s) 769
BstAPI GCANNNNNTGC 1 cut(s) 395
BstBI TTCGAA 1 cut(s) 97
BstC8I GCNNGC 3 cut(s) 155, 340, 519
BstDEI CTNAG 2 cut(s) 501, 680
BstEII GGTNACC 1 cut(s) 295
BstF5I GGATG 3 cut(s) 239, 259, 433
BstHHI GCGC 1 cut(s) 715
BstKTI GATC 6 cut(s) 118, 196, 570, 607, 630, 984
BstMAI GTCTC 2 cut(s) 126, 468
BstMBI GATC 6 cut(s) 115, 193, 567, 604, 627, 981
BstMWI GCNNNNNNNGC 1 cut(s) 395
BstNI CCWGG 1 cut(s) 612
BstNSI RCATGY 1 cut(s) 695
BstPI GGTNACC 1 cut(s) 295
BstSCI CCNGG 2 cut(s) 239, 610
BstSFI CTRYAG 1 cut(s) 309
BstV1I GCAGC 2 cut(s) 398, 484
BstV2I GAAGAC 1 cut(s) 238
BstX2I RGATCY 3 cut(s) 193, 604, 981
BstYI RGATCY 3 cut(s) 193, 604, 981
BsuRI GGCC 1 cut(s) 921
BtsCI GGATG 3 cut(s) 239, 259, 433
BtsIMutI CAGTG 3 cut(s) 90, 624, 845
Cac8I GCNNGC 3 cut(s) 155, 340, 519
CaiI CAGNNNCTG 1 cut(s) 392
CfoI GCGC 1 cut(s) 715
Cfr13I GGNCC 1 cut(s) 17
Csp6I GTAC 6 cut(s) 318, 454, 524, 689, 754, 793
CspCI CAANNNNNGTGG 2 cut(s) 850, 885
CviAII CATG 8 cut(s) 57, 251, 421, 438, 637, 692, 917, 1002
CviQI GTAC 6 cut(s) 318, 454, 524, 689, 754, 793
DdeI CTNAG 2 cut(s) 501, 680
DpnI GATC 6 cut(s) 117, 195, 569, 606, 629, 983
DpnII GATC 6 cut(s) 115, 193, 567, 604, 627, 981
Eam1104I CTCTTC 1 cut(s) 769
EarI CTCTTC 1 cut(s) 769
EciI GGCGGA 1 cut(s) 440
Eco130I CCWWGG 2 cut(s) 322, 583
Eco31I GGTCTC 1 cut(s) 468
Eco47I GGWCC 1 cut(s) 17
Eco91I GGTNACC 1 cut(s) 295
EcoO65I GGTNACC 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 610
EcoT14I CCWWGG 2 cut(s) 322, 583
ErhI CCWWGG 2 cut(s) 322, 583
FaeI CATG 8 cut(s) 60, 254, 424, 441, 640, 695, 920, 1005
FatI CATG 8 cut(s) 56, 250, 420, 437, 636, 691, 916, 1001
Fnu4HI GCNGC 2 cut(s) 387, 498
FokI GGATG 3 cut(s) 246, 246, 440
Fsp4HI GCNGC 2 cut(s) 387, 498
FspAI RTGCGCAY 1 cut(s) 714
FspBI CTAG 4 cut(s) 159, 323, 381, 974
FspI TGCGCA 1 cut(s) 714
GlaI GCGC 1 cut(s) 714
GluI GCNGC 2 cut(s) 387, 498
GsuI CTGGAG 1 cut(s) 960
HaeIII GGCC 1 cut(s) 921
HapII CCGG 1 cut(s) 240
HhaI GCGC 1 cut(s) 715
Hin1II CATG 8 cut(s) 60, 254, 424, 441, 640, 695, 920, 1005
Hin6I GCGC 1 cut(s) 713
HinP1I GCGC 1 cut(s) 713
HindIII AAGCTT 4 cut(s) 71, 173, 336, 855
HinfI GANTC 2 cut(s) 45, 903
HpaII CCGG 1 cut(s) 240
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 2 cut(s) 169, 198
Hpy188III TCNNGA 3 cut(s) 119, 892, 974
Hpy8I GTNNAC 1 cut(s) 373
HpyAV CCTTC 2 cut(s) 224, 308
HpyCH4III ACNGT 4 cut(s) 94, 106, 358, 394
HpyCH4V TGCA 8 cut(s) 79, 126, 237, 386, 536, 600, 809, 957
HpyF10VI GCNNNNNNNGC 1 cut(s) 395
HpyF3I CTNAG 2 cut(s) 501, 680
Hsp92II CATG 8 cut(s) 60, 254, 424, 441, 640, 695, 920, 1005
HspAI GCGC 1 cut(s) 713
KpnI GGTACC 1 cut(s) 321
Kzo9I GATC 6 cut(s) 115, 193, 567, 604, 627, 981
LguI GCTCTTC 1 cut(s) 769
LmnI GCTCC 2 cut(s) 894, 941
Lsp1109I GCAGC 2 cut(s) 398, 484
LweI GCATC 3 cut(s) 173, 224, 299
MaeI CTAG 4 cut(s) 159, 323, 381, 974
MaeIII GTNAC 4 cut(s) 28, 86, 295, 559
MalI GATC 6 cut(s) 117, 195, 569, 606, 629, 983
MboI GATC 6 cut(s) 115, 193, 567, 604, 627, 981
MboII GAAGA 5 cut(s) 17, 175, 238, 756, 895
MfeI CAATTG 1 cut(s) 225
MflI RGATCY 3 cut(s) 193, 604, 981
MluCI AATT 6 cut(s) 225, 530, 623, 733, 821, 864
MmeI TCCRAC 1 cut(s) 797
MnlI CCTC 4 cut(s) 155, 582, 744, 932
MseI TTAA 2 cut(s) 216, 912
MslI CAYNNNNRTG 1 cut(s) 696
MspI CCGG 1 cut(s) 240
MspR9I CCNGG 2 cut(s) 241, 612
MunI CAATTG 1 cut(s) 225
Mva1269I GAATGC 1 cut(s) 128
MvaI CCWGG 1 cut(s) 612
MwoI GCNNNNNNNGC 1 cut(s) 395
NciI CCSGG 1 cut(s) 241
NdeII GATC 6 cut(s) 115, 193, 567, 604, 627, 981
NlaIII CATG 8 cut(s) 60, 254, 424, 441, 640, 695, 920, 1005
NlaIV GGNNCC 2 cut(s) 18, 319
NmuCI GTSAC 1 cut(s) 559
NsbI TGCGCA 1 cut(s) 714
NspI RCATGY 1 cut(s) 695
NspV TTCGAA 1 cut(s) 97
PciI ACATGT 1 cut(s) 691
PciSI GCTCTTC 1 cut(s) 769
PctI GAATGC 1 cut(s) 128
PfeI GAWTC 2 cut(s) 45, 903
PkrI GCNGC 2 cut(s) 388, 499
PscI ACATGT 1 cut(s) 691
Psp6I CCWGG 1 cut(s) 610
PspEI GGTNACC 1 cut(s) 295
PspGI CCWGG 1 cut(s) 610
PspN4I GGNNCC 2 cut(s) 18, 319
PspPI GGNCC 1 cut(s) 17
PstNI CAGNNNCTG 1 cut(s) 392
PsuI RGATCY 3 cut(s) 193, 604, 981
RsaI GTAC 6 cut(s) 319, 455, 525, 690, 755, 794
RsaNI GTAC 6 cut(s) 318, 454, 524, 689, 754, 793
RseI CAYNNNNRTG 1 cut(s) 696
SapI GCTCTTC 1 cut(s) 769
SaqAI TTAA 2 cut(s) 216, 912
SatI GCNGC 2 cut(s) 387, 498
Sau3AI GATC 6 cut(s) 115, 193, 567, 604, 627, 981
Sau96I GGNCC 1 cut(s) 17
ScaI AGTACT 1 cut(s) 525
ScrFI CCNGG 2 cut(s) 241, 612
SfaNI GCATC 3 cut(s) 173, 224, 299
SfcI CTRYAG 1 cut(s) 309
SfuI TTCGAA 1 cut(s) 97
SinI GGWCC 1 cut(s) 17
SmiMI CAYNNNNRTG 1 cut(s) 696
Sse9I AATT 6 cut(s) 225, 530, 623, 733, 821, 864
SsiI CCGC 1 cut(s) 425
SspMI CTAG 4 cut(s) 159, 323, 381, 974
StyD4I CCNGG 2 cut(s) 239, 610
StyI CCWWGG 2 cut(s) 322, 583
TaaI ACNGT 4 cut(s) 94, 106, 358, 394
TaqI TCGA 3 cut(s) 97, 881, 924
TasI AATT 6 cut(s) 225, 530, 623, 733, 821, 864
TatI WGTACW 1 cut(s) 523
TfiI GAWTC 2 cut(s) 45, 903
Tru1I TTAA 2 cut(s) 216, 912
Tru9I TTAA 2 cut(s) 216, 912
TscAI CASTG 3 cut(s) 97, 624, 852
TseFI GTSAC 1 cut(s) 559
TseI GCWGC 2 cut(s) 386, 497
Tsp45I GTSAC 1 cut(s) 559
TspDTI ATGAA 7 cut(s) 37, 45, 83, 300, 426, 474, 583
TspRI CASTG 3 cut(s) 97, 624, 852
VpaK11BI GGWCC 1 cut(s) 17
XapI RAATTY 4 cut(s) 530, 733, 821, 864
XbaI TCTAGA 1 cut(s) 973
XceI RCATGY 1 cut(s) 695
XcmI CCANNNNNNNNNTGG 1 cut(s) 464
XmaJI CCTAGG 1 cut(s) 322
XspI CTAG 4 cut(s) 159, 323, 381, 974
ZrmI AGTACT 1 cut(s) 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.